Lsi02G004970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G004970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cycloeucalenol cycloisomerase-like
Location	chr02: 4214106 .. 4217356 (+)
RNA-Seq Expression	Lsi02G004970
Synteny	Lsi02G004970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTGTCCGCTTTGGAAATTTCGGAACAGCATAGCGAAACGCTACACTCCTCGGTTTTCTCTTATTCAGTTGGAGAATTTCAAGCCGAGATCTATCCTATCATCTCTGTTTTACATCTTAATCACTGTTCTAATGGCCGGTAATTTTGCATTTCTCACTGTCTTCTTCTTCTTTCCTTTCGTTTTCTTCAGCTTCTTACCAATGTCTGTTCAGGTCGTGGCTCCATCTCTAATCATTCTTCAAGCTTATGGCTTTCTTCAAACCCTAGCAAGAGATGGGGTGAACTCTTCTTTCTTTCCTATACTCCATTTTGGCTCACTCTTTGCTTGGGAATTGTCGTTCCTTTCAAACTCTACGAGGTCTGCCCTCATCAACTCTTCTTCAATTTTACTTTCTTTTTAAATCCATATTGCTGCATTTCGATGGATTGTGAAAGACTTTGATGGGTTCACTTGTTTCTTTACATGGGATTTGATTGTTCGCTTCATTTCTGAACTAAGCTGAAGTTTCTTTATCCATTTCTAGATTTTATCTTCTTCTCTGTTCACATTTGGCTTCGTTTTTGAAGAAGTCAACGAACAAGAAAGAGGGCATATTGTTGATTTATACATCTGGGGATTTGACAACCTTCGAAATGGAAATTTGCATTTAATTTTATGAGTGTCATTCAAGAAAAGTGACGTTTAAACTGTCCTGTTTTTCGCAATGAAGACGATTTCTTCTTCTCTATTACGCCTTTACTTTCTATGAGTTGAACTTTTCAAAAGTCTAATCATCGGTGTTTCTTATTGACACAGAGGTTTACGGAATTGGAATATTTGCTTCTTGGATTGTTGGCAGCTCTTCCTTCCTTCTTGATACCGGTGGTGTGGCCTGGAGAGGTATCAATTTACTAGCGCTTCGTTTAAATCTATATGATCCATTACCAAAATTCATTCACATGTTTGTTTCTGGGGACTTATGGATCGCAAGTTAACTAATATGGATCCACATGATTTTTATCCAAGACTGATGGTTCTCTTAGACCGATGTGAAACTGAATATTAGTCACACATAAATAATTAAATCAATCAAGATCTAATCATTAGAAGACAAAGATGAAAGAAGACTTCTCAATTCATCACCTGTAACTTGTGGTGCACAAAGTGATGAAGATAAAGTGATCTTATTTACTTGGTATTTGTTGCAGGCTGACCGCAGTTTGCCTTGGAAAGATCGATATTGGGTAAAGGTTAGTTTTCTACCTTTATTATGTGGATTACTCTCTGTTTTTGCAAGTTAGCAATTCAGAAGAAGTAGCTATATCATCAAAGCTAATTAAGTGTGATAAATGAAAAGTGGTTTCTTTGGCTACAGCCTACAATATTTCTTCTTTTCCCTCCCTTCTTTTGTCCACTTAGATCCTGCTTTTTCATGGCTACTCTTTCCAATCAGCTATGATAACTGAGAATCTCTTTCAACCTCTTATCTAGTACTTTTTCTCTCCATAAGATTTTGGTATATATACTTTGATCGATGGCTTCAACATTTGTTGGCGCATCCCTTTGGGCTAAGAAAATTTCTTATCCATTCCAACCTCTACTCGTTTTTGCTCACTTTACAAAGCCAAGTTATCTATCATCACTCAATTGCATTTTTAACACTAGTTTAGCATTTGCTCAATAAATGTGTATCTGACTGAAAATACTTATCTAGGCATATTCTATCAGATTTCTAGAGAGGAAAATTTGTTGGGTGACTTTGAGCTGGTGAACATTTTATATTGCAGATGAAAACGTGATAAGAATGCTTGTTTCTTTTCTTCTCCTCTTTTTAAAATAATAACAATAACAATAATAATAATAACAACAATGCAATTTTCCGGAGAAATATTAGCCCTCCTGATTCTGAGTATGGAGAGACGGCAAGTAATAACATATTTTGACTGAGTTCCATATAATCCAATTCCTATAGTGTTGTATCACTTAACCGTCAAGTTAGACACTACCAATCAGTTTTTCAATAAAGTCAGTTTGTTACCTCTACATAATAATTTTGGTTCTTCTTTGTGGCAGGCTAGCCTTTGGATAGTAATCTTCAGCTATGTTGGAAATTACTTTTGGACCCACTACTTTTTCACTGTTTTGGGGGCATCTTATACCTTTCCATCATGGAGGATGAATAATGTAAGCATGGGCAATTGAAGGGCAAAGTATAAAAAAAAGCATTGATTGCCTGCTTCTATTTCTAAAAAATGATTTGTTAATCACAGGTACCACACACGACCTTTCTTCTCACGCATGTATGCTTTCTGTTCTATCACGTCGCATCTAACATGACTCTTCGGAGACTTCGCCATTCTGTTGCCGACTTGCCGGAGAATATTCAATGGTTTACACTGGTTGCATGGATTTTGGCACTCTCATACTTCATTGCATACTTGGAGACTCTGGCCATTTCTAATGTAATTTCTCATATTTCTCCTTTTGTGTTGTCGATAAGTATCTTTTCAATCTGTCTCATTGTGTGCTTGAGTTATTTGAACTGTAAGGATTGAGAACTTCAGTATATTTTTATAAAACCATTTCCACGAAACTCTTTCTGATATATGCTTCGAGATATCTTTTCACACTTTCTGTTGTAGTTTTTCTTCATATTCCTTCTCATTTTTAATAACTTGAAACATCTGAAGTTTTATTTATTAACATAGAAGAATCGTTCTTACCAATGATTTTTTAATACACATCTTACTTTGAGCCTGTGCAGTTCCCTTACTACCAATTTGTTGATCGAGCTTCAATGTACAAAGTTGGCTCTTTGTTTTATGCCATCTACTTCTTCGTCAGCTTCCCAATGTTCTTGAGGTAAGATTCTCTATCCTAGTGCCTTAATATGCATAAATCAGTTTGTTTATAATAATAGAAACCACATACAAGTCTACCATTCCCTCCATCAATCTGTCCTCAAAAGAAATTGATGACTTCAAGACTGCAGACATGAATGACACAAACTAGATAAGGGAAGAAAGACAACGAAAGAGTTGTAGAACTTGTGATTTTTTGGGACACTTCAATTTTCTATGACCGATCTGAATCATCTTCTGTAACTGTTATGCTGCCGCCGCCTCTTCTATTGCCCATAATATGCATGAACTTATGGAGTACATTGACTTTTTTTTTTAATCTTATCCCAGGCGTTTGTTCATGGCTATATTACCACCCTTTACATTAGCCGCAATTTCATTATGGCATCCAATACACATGAAGCCATAG

mRNA sequence

AGGTGTCCGCTTTGGAAATTTCGGAACAGCATAGCGAAACGCTACACTCCTCGGTTTTCTCTTATTCAGTTGGAGAATTTCAAGCCGAGATCTATCCTATCATCTCTGTTTTACATCTTAATCACTGTTCTAATGGCCGGTCGTGGCTCCATCTCTAATCATTCTTCAAGCTTATGGCTTTCTTCAAACCCTAGCAAGAGATGGGGTGAACTCTTCTTTCTTTCCTATACTCCATTTTGGCTCACTCTTTGCTTGGGAATTGTCGTTCCTTTCAAACTCTACGAGATTTTATCTTCTTCTCTGTTCACATTTGGCTTCGTTTTTGAAGAAAGGTTTACGGAATTGGAATATTTGCTTCTTGGATTGTTGGCAGCTCTTCCTTCCTTCTTGATACCGGTGGTGTGGCCTGGAGAGACCGATGCTGACCGCAGTTTGCCTTGGAAAGATCGATATTGGGTAAAGATGAAAACTGTTGTATCACTTAACCGTCAAGCTAGCCTTTGGATAGTAATCTTCAGCTATGTTGGAAATTACTTTTGGACCCACTACTTTTTCACTGTTTTGGGGGCATCTTATACCTTTCCATCATGGAGGATGAATAATGTACCACACACGACCTTTCTTCTCACGCATGTATGCTTTCTGTTCTATCACGTCGCATCTAACATGACTCTTCGGAGACTTCGCCATTCTGTTGCCGACTTGCCGGAGAATATTCAATGGTTTACACTGGTTGCATGGATTTTGGCACTCTCATACTTCATTGCATACTTGGAGACTCTGGCCATTTCTAATTTCCCTTACTACCAATTTGTTGATCGAGCTTCAATGTACAAAGTTGGCTCTTTGTTTTATGCCATCTACTTCTTCGTCAGCTTCCCAATGTTCTTGAGGCGTTTGTTCATGGCTATATTACCACCCTTTACATTAGCCGCAATTTCATTATGGCATCCAATACACATGAAGCCATAG

Coding sequence (CDS)

AGGTGTCCGCTTTGGAAATTTCGGAACAGCATAGCGAAACGCTACACTCCTCGGTTTTCTCTTATTCAGTTGGAGAATTTCAAGCCGAGATCTATCCTATCATCTCTGTTTTACATCTTAATCACTGTTCTAATGGCCGGTCGTGGCTCCATCTCTAATCATTCTTCAAGCTTATGGCTTTCTTCAAACCCTAGCAAGAGATGGGGTGAACTCTTCTTTCTTTCCTATACTCCATTTTGGCTCACTCTTTGCTTGGGAATTGTCGTTCCTTTCAAACTCTACGAGATTTTATCTTCTTCTCTGTTCACATTTGGCTTCGTTTTTGAAGAAAGGTTTACGGAATTGGAATATTTGCTTCTTGGATTGTTGGCAGCTCTTCCTTCCTTCTTGATACCGGTGGTGTGGCCTGGAGAGACCGATGCTGACCGCAGTTTGCCTTGGAAAGATCGATATTGGGTAAAGATGAAAACTGTTGTATCACTTAACCGTCAAGCTAGCCTTTGGATAGTAATCTTCAGCTATGTTGGAAATTACTTTTGGACCCACTACTTTTTCACTGTTTTGGGGGCATCTTATACCTTTCCATCATGGAGGATGAATAATGTACCACACACGACCTTTCTTCTCACGCATGTATGCTTTCTGTTCTATCACGTCGCATCTAACATGACTCTTCGGAGACTTCGCCATTCTGTTGCCGACTTGCCGGAGAATATTCAATGGTTTACACTGGTTGCATGGATTTTGGCACTCTCATACTTCATTGCATACTTGGAGACTCTGGCCATTTCTAATTTCCCTTACTACCAATTTGTTGATCGAGCTTCAATGTACAAAGTTGGCTCTTTGTTTTATGCCATCTACTTCTTCGTCAGCTTCCCAATGTTCTTGAGGCGTTTGTTCATGGCTATATTACCACCCTTTACATTAGCCGCAATTTCATTATGGCATCCAATACACATGAAGCCATAG

Protein sequence

RCPLWKFRNSIAKRYTPRFSLIQLENFKPRSILSSLFYILITVLMAGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFLRRLFMAILPPFTLAAISLWHPIHMKP

Homology

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BLAST of Lsi02G004970 vs. ExPASy Swiss-Prot
Match: Q9M643 (Cycloeucalenol cycloisomerase OS=Arabidopsis thaliana OX=3702 GN=CPI1 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.6e-95
Identity = 174/244 (71.31%), Postives = 199/244 (81.56%), Query Frame = 0


Query: 55  SSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTE 114
           S SLWL+ NPSKRWGELFFL YTPFWLTLCLGIVVP+KLY               E FTE
Sbjct: 6   SPSLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLY---------------ETFTE 65

Query: 115 LEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSY 174
           LEYLLL L++A+P+F+IP++  G+  ADRSL WKDRYWVK          A+LWI++FSY
Sbjct: 66  LEYLLLALVSAVPAFVIPMLLVGK--ADRSLCWKDRYWVK----------ANLWIIVFSY 125

Query: 175 VGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVAD 234
           VGNYFWTHYFF VLGASYTFPSW+MNNVPHTTF LTHVCFLFYHVASN+TLRRLRHS AD
Sbjct: 126 VGNYFWTHYFFKVLGASYTFPSWKMNNVPHTTFFLTHVCFLFYHVASNITLRRLRHSTAD 185

Query: 235 LPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFP 294
           LP++++W    AWILALSYFIAYLET+AI+NFPYY+FVDR++MY+VG LFYAIYF VSFP
Sbjct: 186 LPDSLKWCFEAAWILALSYFIAYLETIAIANFPYYEFVDRSAMYRVGCLFYAIYFIVSFP 222

Query: 295 MFLR 299
           MF R
Sbjct: 246 MFFR 222

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BLAST of Lsi02G004970 vs. ExPASy TrEMBL
Match: A0A6J1E6Z0 (cycloeucalenol cycloisomerase-like OS=Cucurbita moschata OX=3662 GN=LOC111431284 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.2e-122
Identity = 234/297 (78.79%), Postives = 246/297 (82.83%), Query Frame = 0


Query: 2   CPLWKFRNSIAKRYTPRFSLIQLENFKPRSILSSLFYILITVLMAGRGSISNHSSSLWLS 61
           C  W FR    KR++PRFSLIQLENFKPRSI   L +   +++ AGR S  N SSSLWLS
Sbjct: 25  CQSWNFR----KRFSPRFSLIQLENFKPRSISLDLNH---SLMAAGRRSARNQSSSLWLS 84

Query: 62  SNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTELEYLLLG 121
           SNPSKRWGE+FFLSYTPFWLTLCLGIVVPFKLY               E FTELEYLLLG
Sbjct: 85  SNPSKRWGEVFFLSYTPFWLTLCLGIVVPFKLY---------------ESFTELEYLLLG 144

Query: 122 LLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSYVGNYFWT 181
           L+AALPSFLIPVVWPGE  ADRSLPWK+RYWVK          ASLWIVIFSYVGNYFWT
Sbjct: 145 LVAALPSFLIPVVWPGE--ADRSLPWKERYWVK----------ASLWIVIFSYVGNYFWT 204

Query: 182 HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVADLPENIQW 241
           HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHV SNMTLRRLRHS+ADLPENIQW
Sbjct: 205 HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVTSNMTLRRLRHSIADLPENIQW 264

Query: 242 FTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFLR 299
             L AWILALSYFIAYLET+AISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMF+R
Sbjct: 265 VMLGAWILALSYFIAYLETVAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFMR 287

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BLAST of Lsi02G004970 vs. ExPASy TrEMBL
Match: A0A0A0LUD1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050410 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.9e-115
Identity = 215/253 (84.98%), Postives = 222/253 (87.75%), Query Frame = 0


Query: 46  AGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFG 105
           +G  + ++HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIV+PFKLY           
Sbjct: 5   SGSSTSNHHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLY----------- 64

Query: 106 FVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQA 165
               ERFTELEYLLLGLLAALP+FLIPVVWPGE  ADR LPWKDRYWVK          A
Sbjct: 65  ----ERFTELEYLLLGLLAALPAFLIPVVWPGE--ADRGLPWKDRYWVK----------A 124

Query: 166 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTL 225
           SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMN+VPHTTFLLTHVCFLFYHVASNMTL
Sbjct: 125 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNDVPHTTFLLTHVCFLFYHVASNMTL 184

Query: 226 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 285
           RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY
Sbjct: 185 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 230

Query: 286 AIYFFVSFPMFLR 299
           AIYFFVSFPMFLR
Sbjct: 245 AIYFFVSFPMFLR 230

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BLAST of Lsi02G004970 vs. ExPASy TrEMBL
Match: A0A5A7UM17 (Cycloeucalenol cycloisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001690 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.8e-114
Identity = 214/253 (84.58%), Postives = 221/253 (87.35%), Query Frame = 0


Query: 46  AGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFG 105
           +G  + ++HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIV+PFKLY           
Sbjct: 5   SGSSTSNHHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLY----------- 64

Query: 106 FVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQA 165
               ERFTELEYLLLGLLAALP+FLIPVVWPGE  ADR LPWKDRYWVK          A
Sbjct: 65  ----ERFTELEYLLLGLLAALPAFLIPVVWPGE--ADRCLPWKDRYWVK----------A 124

Query: 166 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTL 225
           SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMN+VPHTTFLLTHVCFLFYHVASNMTL
Sbjct: 125 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNDVPHTTFLLTHVCFLFYHVASNMTL 184

Query: 226 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 285
           RRLRHSVADLPENIQW TLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY
Sbjct: 185 RRLRHSVADLPENIQWVTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 230

Query: 286 AIYFFVSFPMFLR 299
           AIYFFVSFPMFLR
Sbjct: 245 AIYFFVSFPMFLR 230

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BLAST of Lsi02G004970 vs. ExPASy TrEMBL
Match: A0A1S3AZ59 (cycloeucalenol cycloisomerase OS=Cucumis melo OX=3656 GN=LOC103484362 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.8e-114
Identity = 214/253 (84.58%), Postives = 221/253 (87.35%), Query Frame = 0


Query: 46  AGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFG 105
           +G  + ++HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIV+PFKLY           
Sbjct: 5   SGSSTSNHHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLY----------- 64

Query: 106 FVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQA 165
               ERFTELEYLLLGLLAALP+FLIPVVWPGE  ADR LPWKDRYWVK          A
Sbjct: 65  ----ERFTELEYLLLGLLAALPAFLIPVVWPGE--ADRCLPWKDRYWVK----------A 124

Query: 166 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTL 225
           SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMN+VPHTTFLLTHVCFLFYHVASNMTL
Sbjct: 125 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNDVPHTTFLLTHVCFLFYHVASNMTL 184

Query: 226 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 285
           RRLRHSVADLPENIQW TLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY
Sbjct: 185 RRLRHSVADLPENIQWVTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 230

Query: 286 AIYFFVSFPMFLR 299
           AIYFFVSFPMFLR
Sbjct: 245 AIYFFVSFPMFLR 230

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BLAST of Lsi02G004970 vs. ExPASy TrEMBL
Match: A0A6J1KJU7 (cycloeucalenol cycloisomerase-like OS=Cucurbita maxima OX=3661 GN=LOC111495263 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 2.7e-111
Identity = 210/255 (82.35%), Postives = 218/255 (85.49%), Query Frame = 0


Query: 44  LMAGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFT 103
           + AGR S  + SSSLWLSSNPSKRWGE+FFLSYTPFWLTLCLGIVVPFKLY         
Sbjct: 1   MAAGRRSARDQSSSLWLSSNPSKRWGEVFFLSYTPFWLTLCLGIVVPFKLY--------- 60

Query: 104 FGFVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNR 163
                 E FTELEYLLLGL+AALPSFLIPVVWPGE  ADRSLPWK+RYWVK         
Sbjct: 61  ------ESFTELEYLLLGLVAALPSFLIPVVWPGE--ADRSLPWKERYWVK--------- 120

Query: 164 QASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNM 223
            ASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHV SNM
Sbjct: 121 -ASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVTSNM 180

Query: 224 TLRRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSL 283
           TLRRLRHS+ADLPENIQW  L AWILALSYFIAYLET+AISNFPYYQFVDRASMYKVGSL
Sbjct: 181 TLRRLRHSIADLPENIQWVMLGAWILALSYFIAYLETVAISNFPYYQFVDRASMYKVGSL 228

Query: 284 FYAIYFFVSFPMFLR 299
           FYAIYFFVSFPMF+R
Sbjct: 241 FYAIYFFVSFPMFMR 228

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BLAST of Lsi02G004970 vs. NCBI nr
Match: XP_022923634.1 (cycloeucalenol cycloisomerase-like [Cucurbita moschata])

HSP 1 Score: 449.5 bits (1155), Expect = 2.4e-122
Identity = 234/297 (78.79%), Postives = 246/297 (82.83%), Query Frame = 0


Query: 2   CPLWKFRNSIAKRYTPRFSLIQLENFKPRSILSSLFYILITVLMAGRGSISNHSSSLWLS 61
           C  W FR    KR++PRFSLIQLENFKPRSI   L +   +++ AGR S  N SSSLWLS
Sbjct: 25  CQSWNFR----KRFSPRFSLIQLENFKPRSISLDLNH---SLMAAGRRSARNQSSSLWLS 84

Query: 62  SNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTELEYLLLG 121
           SNPSKRWGE+FFLSYTPFWLTLCLGIVVPFKLY               E FTELEYLLLG
Sbjct: 85  SNPSKRWGEVFFLSYTPFWLTLCLGIVVPFKLY---------------ESFTELEYLLLG 144

Query: 122 LLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSYVGNYFWT 181
           L+AALPSFLIPVVWPGE  ADRSLPWK+RYWVK          ASLWIVIFSYVGNYFWT
Sbjct: 145 LVAALPSFLIPVVWPGE--ADRSLPWKERYWVK----------ASLWIVIFSYVGNYFWT 204

Query: 182 HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVADLPENIQW 241
           HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHV SNMTLRRLRHS+ADLPENIQW
Sbjct: 205 HYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVTSNMTLRRLRHSIADLPENIQW 264

Query: 242 FTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFLR 299
             L AWILALSYFIAYLET+AISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMF+R
Sbjct: 265 VMLGAWILALSYFIAYLETVAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFMR 287

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BLAST of Lsi02G004970 vs. NCBI nr
Match: KAG6584146.1 (Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.0 bits (1120), Expect = 2.8e-118
Identity = 231/292 (79.11%), Postives = 242/292 (82.88%), Query Frame = 0


Query: 9   NSIAKR--YTPRFSLIQLENFKPRSILSSLFYILITVLMAGRGSISNHSSSLWLSSNPSK 68
           N IA R  +TPRF LIQLENFKPRSI   L +   +++ AGR S  N SSSLWLSSNPSK
Sbjct: 26  NIIATRLFHTPRFPLIQLENFKPRSIGLDLNH---SLMAAGRRSARNQSSSLWLSSNPSK 85

Query: 69  RWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTELEYLLLGLLAAL 128
           R GE+FFLSYTPFWLTLCLGIVVPFKLY               E FTELEYLLLGL+AAL
Sbjct: 86  RCGEVFFLSYTPFWLTLCLGIVVPFKLY---------------ESFTELEYLLLGLVAAL 145

Query: 129 PSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSYVGNYFWTHYFFT 188
           PSFLIPVVWPGE  ADRSLPWK+RYWVK          ASLWIVIFSYVGNYFWTHYFFT
Sbjct: 146 PSFLIPVVWPGE--ADRSLPWKERYWVK----------ASLWIVIFSYVGNYFWTHYFFT 205

Query: 189 VLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVADLPENIQWFTLVA 248
           VLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHV SNMTLRRLRHS+ADLPENIQW  L A
Sbjct: 206 VLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVTSNMTLRRLRHSIADLPENIQWVMLGA 265

Query: 249 WILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFLR 299
           WILALSYFIAYLET+AISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMF+R
Sbjct: 266 WILALSYFIAYLETVAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFPMFMR 287

[Close]

BLAST of Lsi02G004970 vs. NCBI nr
Match: XP_004152524.1 (cycloeucalenol cycloisomerase [Cucumis sativus] >KGN64402.1 hypothetical protein Csa_013530 [Cucumis sativus])

HSP 1 Score: 423.7 bits (1088), Expect = 1.4e-114
Identity = 215/253 (84.98%), Postives = 222/253 (87.75%), Query Frame = 0


Query: 46  AGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFG 105
           +G  + ++HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIV+PFKLY           
Sbjct: 5   SGSSTSNHHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLY----------- 64

Query: 106 FVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQA 165
               ERFTELEYLLLGLLAALP+FLIPVVWPGE  ADR LPWKDRYWVK          A
Sbjct: 65  ----ERFTELEYLLLGLLAALPAFLIPVVWPGE--ADRGLPWKDRYWVK----------A 124

Query: 166 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTL 225
           SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMN+VPHTTFLLTHVCFLFYHVASNMTL
Sbjct: 125 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNDVPHTTFLLTHVCFLFYHVASNMTL 184

Query: 226 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 285
           RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY
Sbjct: 185 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 230

Query: 286 AIYFFVSFPMFLR 299
           AIYFFVSFPMFLR
Sbjct: 245 AIYFFVSFPMFLR 230

[Close]

BLAST of Lsi02G004970 vs. NCBI nr
Match: XP_038893628.1 (cycloeucalenol cycloisomerase [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 9.2e-114
Identity = 217/254 (85.43%), Postives = 220/254 (86.61%), Query Frame = 0


Query: 45  MAGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTF 104
           MAG  S+S HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLY          
Sbjct: 1   MAGPASVSKHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLY---------- 60

Query: 105 GFVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQ 164
                ERFTELEYLL GLLAALPSFLIPVVWPGE  ADRSLPWKDRYWVK          
Sbjct: 61  -----ERFTELEYLLSGLLAALPSFLIPVVWPGE--ADRSLPWKDRYWVK---------- 120

Query: 165 ASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMT 224
           ASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMT
Sbjct: 121 ASLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMT 180

Query: 225 LRRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLF 284
           LRRLRHS+ADLPENIQ   LVAWI+ALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLF
Sbjct: 181 LRRLRHSIADLPENIQRVMLVAWIVALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLF 227

Query: 285 YAIYFFVSFPMFLR 299
           YAIYFFVSFPMFLR
Sbjct: 241 YAIYFFVSFPMFLR 227

[Close]

BLAST of Lsi02G004970 vs. NCBI nr
Match: XP_008439629.1 (PREDICTED: cycloeucalenol cycloisomerase [Cucumis melo] >KAA0055326.1 cycloeucalenol cycloisomerase [Cucumis melo var. makuwa])

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-113
Identity = 214/253 (84.58%), Postives = 221/253 (87.35%), Query Frame = 0


Query: 46  AGRGSISNHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFG 105
           +G  + ++HSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIV+PFKLY           
Sbjct: 5   SGSSTSNHHSSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLY----------- 64

Query: 106 FVFEERFTELEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQA 165
               ERFTELEYLLLGLLAALP+FLIPVVWPGE  ADR LPWKDRYWVK          A
Sbjct: 65  ----ERFTELEYLLLGLLAALPAFLIPVVWPGE--ADRCLPWKDRYWVK----------A 124

Query: 166 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTL 225
           SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMN+VPHTTFLLTHVCFLFYHVASNMTL
Sbjct: 125 SLWIVIFSYVGNYFWTHYFFTVLGASYTFPSWRMNDVPHTTFLLTHVCFLFYHVASNMTL 184

Query: 226 RRLRHSVADLPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 285
           RRLRHSVADLPENIQW TLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY
Sbjct: 185 RRLRHSVADLPENIQWVTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFY 230

Query: 286 AIYFFVSFPMFLR 299
           AIYFFVSFPMFLR
Sbjct: 245 AIYFFVSFPMFLR 230

[Close]

BLAST of Lsi02G004970 vs. TAIR 10
Match: AT5G50375.1 (cyclopropyl isomerase )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 174/244 (71.31%), Postives = 199/244 (81.56%), Query Frame = 0


Query: 55  SSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTE 114
           S SLWL+ NPSKRWGELFFL YTPFWLTLCLGIVVP+KLY               E FTE
Sbjct: 6   SPSLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLY---------------ETFTE 65

Query: 115 LEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSY 174
           LEYLLL L++A+P+F+IP++  G+  ADRSL WKDRYWVK          A+LWI++FSY
Sbjct: 66  LEYLLLALVSAVPAFVIPMLLVGK--ADRSLCWKDRYWVK----------ANLWIIVFSY 125

Query: 175 VGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVAD 234
           VGNYFWTHYFF VLGASYTFPSW+MNNVPHTTF LTHVCFLFYHVASN+TLRRLRHS AD
Sbjct: 126 VGNYFWTHYFFKVLGASYTFPSWKMNNVPHTTFFLTHVCFLFYHVASNITLRRLRHSTAD 185

Query: 235 LPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFP 294
           LP++++W    AWILALSYFIAYLET+AI+NFPYY+FVDR++MY+VG LFYAIYF VSFP
Sbjct: 186 LPDSLKWCFEAAWILALSYFIAYLETIAIANFPYYEFVDRSAMYRVGCLFYAIYFIVSFP 222

Query: 295 MFLR 299
           MF R
Sbjct: 246 MFFR 222

[Close]

BLAST of Lsi02G004970 vs. TAIR 10
Match: AT5G50375.2 (cyclopropyl isomerase )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 174/244 (71.31%), Postives = 199/244 (81.56%), Query Frame = 0


Query: 55  SSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVVPFKLYEILSSSLFTFGFVFEERFTE 114
           S SLWL+ NPSKRWGELFFL YTPFWLTLCLGIVVP+KLY               E FTE
Sbjct: 6   SPSLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLY---------------ETFTE 65

Query: 115 LEYLLLGLLAALPSFLIPVVWPGETDADRSLPWKDRYWVKMKTVVSLNRQASLWIVIFSY 174
           LEYLLL L++A+P+F+IP++  G+  ADRSL WKDRYWVK          A+LWI++FSY
Sbjct: 66  LEYLLLALVSAVPAFVIPMLLVGK--ADRSLCWKDRYWVK----------ANLWIIVFSY 125

Query: 175 VGNYFWTHYFFTVLGASYTFPSWRMNNVPHTTFLLTHVCFLFYHVASNMTLRRLRHSVAD 234
           VGNYFWTHYFF VLGASYTFPSW+MNNVPHTTF LTHVCFLFYHVASN+TLRRLRHS AD
Sbjct: 126 VGNYFWTHYFFKVLGASYTFPSWKMNNVPHTTFFLTHVCFLFYHVASNITLRRLRHSTAD 185

Query: 235 LPENIQWFTLVAWILALSYFIAYLETLAISNFPYYQFVDRASMYKVGSLFYAIYFFVSFP 294
           LP++++W    AWILALSYFIAYLET+AI+NFPYY+FVDR++MY+VG LFYAIYF VSFP
Sbjct: 186 LPDSLKWCFEAAWILALSYFIAYLETIAIANFPYYEFVDRSAMYRVGCLFYAIYFIVSFP 222

Query: 295 MFLR 299
           MF R
Sbjct: 246 MFFR 222

The following BLAST results are available for this feature:

BLAST of Lsi02G004970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M643	2.6e-95	71.31	Cycloeucalenol cycloisomerase OS=Arabidopsis thaliana OX=3702 GN=CPI1 PE=2 SV=1	[more]

BLAST of Lsi02G004970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E6Z0	1.2e-122	78.79	cycloeucalenol cycloisomerase-like OS=Cucurbita moschata OX=3662 GN=LOC111431284...	[more]
A0A0A0LUD1	6.9e-115	84.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050410 PE=4 SV=1	[more]
A0A5A7UM17	5.8e-114	84.58	Cycloeucalenol cycloisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A1S3AZ59	5.8e-114	84.58	cycloeucalenol cycloisomerase OS=Cucumis melo OX=3656 GN=LOC103484362 PE=4 SV=1	[more]
A0A6J1KJU7	2.7e-111	82.35	cycloeucalenol cycloisomerase-like OS=Cucurbita maxima OX=3661 GN=LOC111495263 P...	[more]

BLAST of Lsi02G004970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923634.1	2.4e-122	78.79	cycloeucalenol cycloisomerase-like [Cucurbita moschata]	[more]
KAG6584146.1	2.8e-118	79.11	Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004152524.1	1.4e-114	84.98	cycloeucalenol cycloisomerase [Cucumis sativus] >KGN64402.1 hypothetical protein...	[more]
XP_038893628.1	9.2e-114	85.43	cycloeucalenol cycloisomerase [Benincasa hispida]	[more]
XP_008439629.1	1.2e-113	84.58	PREDICTED: cycloeucalenol cycloisomerase [Cucumis melo] >KAA0055326.1 cycloeucal...	[more]

BLAST of Lsi02G004970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50375.1	1.9e-96	71.31	cyclopropyl isomerase	[more]
AT5G50375.2	1.9e-96	71.31	cyclopropyl isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020532	Cycloeucalenol cycloisomerase	PANTHER	PTHR35136	CYCLOEUCALENOL CYCLOISOMERASE	coord: 50..298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G004970.1	Lsi02G004970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0047793	cycloeucalenol cycloisomerase activity