Lsi02G003900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G003900
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Heme-binding protein 2-like
Location	chr02: 3326058 .. 3327245 (-)
RNA-Seq Expression	Lsi02G003900
Synteny	Lsi02G003900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GATAATTGAAAGTTAAAGAGAGTGGAAACAGATGAAGAGGTCAGAATCGACGAACTTACTTGAAAGCTGTAACGTGCGGACAGATTACAGTCTGAGTAGTAATAGGAATTGGTTTGAAGAGTTTTCACCATCTATCCCATCATCCTATGATTGATGATTGATAGAGGGAGAGTGAGAGAGAAAGAGAGATCTTGGGCTTATTTGAAATGGGCCTTGCCATAGCCTATACGGCCCAAATGATGCTCTCGACTGGATGCACTGCTCCGAATGAAATTCAACAACGAATTTGGTAACAAGAGCGGAGAGAGAAAGAGGTCCCAGAACAAGAATCAAGCAGGATGGAGAATCCGAAATTATCAATCTTTCTTGTCAACCTCTTGTGTTTTCTCTGTCTTTGCATCGCCATTGAATCACCCCAATACGCTGTTGTTCATTCAGAATCAGACTTCGAAATCAGATTTTACAGAAAGTCCACTTGGATGTCTGCTCCTGTCCAAGACCCATCCTTCGAGAAAGCTACGAAAAATGGTTTCCACAGGTAATTCCATTTCCAGTTTCCACTTCCACCGCATATTTCAAATCCCATCAACCCCAAATTTACTCTTCCTCATCGATTCTGAGTAAACTTGGGAAAGAAGAAAGCATATCTAAAAATTACCTAAAATTGACTCTGTTTGTGATCATCTTCAATCGTCCCTTTGGTTGATCAAACCTTTTATAATGATCATATTGCACAGACTGTTCCAATACATACAAGGTGCTAATCTGAATTGGTCAAGGATTGCCATGACTGCTCCTGTCTTGATAAGCATTATCCCTGGGGCTGGCCCTCTTCACTCCTCCTCTTATCTTGTTCAATTCTATCTGCCACTTAAGTTTCAAGCTTCACCCCCTCTTCCTCTTCCTGAACTGAAACTGAAGCCCTGTGGTTGGGAAGCTCATTGTGTTGCAGTTAGAAAGTTCTCTGGATTTTCAAGGGATGAGAAGGTCATAAAGGAAGCAGAGAAACTTGCCACCAGCTTGAGTAGGTTCCCTTTGGCGAATTCCACCTCTGAAAGGGGTTATGCATATGCCATTGCCCAATACGACTCTCCCTTCCGGTTGATCGGACGAGTTAATGAAGTCTGGGTCGATGTCGATGCAGCTGATTTGGATGGATGTAAATCCAGTGCCATTGCCGCATTCTAA

mRNA sequence

GATAATTGAAAGTTAAAGAGAGTGGAAACAGATGAAGAGGTCAGAATCGACGAACTTACTTGAAAGCTGTAACGTGCGGACAGATTACAGTCTGAGTAGTAATAGGAATTGGTTTGAAGAGTTTTCACCATCTATCCCATCATCCTATGATTGATGATTGATAGAGGGAGAGTGAGAGAGAAAGAGAGATCTTGGGCTTATTTGAAATGGGCCTTGCCATAGCCTATACGGCCCAAATGATGCTCTCGACTGGATGCACTGCTCCGAATGAAATTCAACAACGAATTTGGTAACAAGAGCGGAGAGAGAAAGAGGTCCCAGAACAAGAATCAAGCAGGATGGAGAATCCGAAATTATCAATCTTTCTTGTCAACCTCTTGTGTTTTCTCTGTCTTTGCATCGCCATTGAATCACCCCAATACGCTGTTGTTCATTCAGAATCAGACTTCGAAATCAGATTTTACAGAAAGTCCACTTGGATGTCTGCTCCTGTCCAAGACCCATCCTTCGAGAAAGCTACGAAAAATGGTTTCCACAGACTGTTCCAATACATACAAGGTGCTAATCTGAATTGGTCAAGGATTGCCATGACTGCTCCTGTCTTGATAAGCATTATCCCTGGGGCTGGCCCTCTTCACTCCTCCTCTTATCTTGTTCAATTCTATCTGCCACTTAAGTTTCAAGCTTCACCCCCTCTTCCTCTTCCTGAACTGAAACTGAAGCCCTGTGGTTGGGAAGCTCATTGTGTTGCAGTTAGAAAGTTCTCTGGATTTTCAAGGGATGAGAAGGTCATAAAGGAAGCAGAGAAACTTGCCACCAGCTTGAGTAGGTTCCCTTTGGCGAATTCCACCTCTGAAAGGGGTTATGCATATGCCATTGCCCAATACGACTCTCCCTTCCGGTTGATCGGACGAGTTAATGAAGTCTGGGTCGATGTCGATGCAGCTGATTTGGATGGATGTAAATCCAGTGCCATTGCCGCATTCTAA

Coding sequence (CDS)

ATGGAGAATCCGAAATTATCAATCTTTCTTGTCAACCTCTTGTGTTTTCTCTGTCTTTGCATCGCCATTGAATCACCCCAATACGCTGTTGTTCATTCAGAATCAGACTTCGAAATCAGATTTTACAGAAAGTCCACTTGGATGTCTGCTCCTGTCCAAGACCCATCCTTCGAGAAAGCTACGAAAAATGGTTTCCACAGACTGTTCCAATACATACAAGGTGCTAATCTGAATTGGTCAAGGATTGCCATGACTGCTCCTGTCTTGATAAGCATTATCCCTGGGGCTGGCCCTCTTCACTCCTCCTCTTATCTTGTTCAATTCTATCTGCCACTTAAGTTTCAAGCTTCACCCCCTCTTCCTCTTCCTGAACTGAAACTGAAGCCCTGTGGTTGGGAAGCTCATTGTGTTGCAGTTAGAAAGTTCTCTGGATTTTCAAGGGATGAGAAGGTCATAAAGGAAGCAGAGAAACTTGCCACCAGCTTGAGTAGGTTCCCTTTGGCGAATTCCACCTCTGAAAGGGGTTATGCATATGCCATTGCCCAATACGACTCTCCCTTCCGGTTGATCGGACGAGTTAATGAAGTCTGGGTCGATGTCGATGCAGCTGATTTGGATGGATGTAAATCCAGTGCCATTGCCGCATTCTAA

Protein sequence

MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKATKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAIAQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF

Homology

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BLAST of Lsi02G003900 vs. ExPASy Swiss-Prot
Match: Q6NU05 (Heme-binding protein 1 OS=Xenopus laevis OX=8355 GN=hebp1 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 47/161 (29.19%), Postives = 77/161 (47.83%), Query Frame = 0


Query: 37  FEIRFYRKSTWMSAPVQDPSFEKATKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGA 96
           FE R Y    ++S  V    F++A+K G  RL +Y+ G+N   + + MT+PV+I+  P  
Sbjct: 31  FEEREYEGGKFISTEVSGKPFDEASKEGVLRLLKYVGGSNNKSAGMGMTSPVIINSYPSE 90

Query: 97  GPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAE 156
                 +  V   +P ++QA PP+P  +  ++    E+  +   +F G++++   +  A 
Sbjct: 91  NDTLQPNVKVLLRIPSQYQADPPVP-TDNTIQIEDRESVTLYSTQFGGYAKEADYVSHAA 150

Query: 157 KLATSLSRFPLANSTSERGYAYAIAQYDSPFRLIGRVNEVW 198
           KL     R  L    S     Y    YD P +  GR NEVW
Sbjct: 151 KL-----RSCLGPDISYHSDYYMCCGYDPPMKPYGRRNEVW 185

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BLAST of Lsi02G003900 vs. ExPASy Swiss-Prot
Match: Q9R257 (Heme-binding protein 1 OS=Mus musculus OX=10090 GN=Hebp1 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.1e-11
Identity = 45/162 (27.78%), Postives = 73/162 (45.06%), Query Frame = 0


Query: 37  FEIRFYRKSTWMSAPVQDPSFEKATKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGA 96
           +E R      + +  V D   ++A +    ++ +Y+ G N     + MT PV  ++ P  
Sbjct: 31  YEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGTNDKGVGMGMTVPVSFALFPNE 90

Query: 97  GPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAE 156
                    V F +P +FQ SPP P  E  +K    E   V   +F G++++   +  A 
Sbjct: 91  DGSLQKKLKVWFRIPNQFQGSPPAPSDE-SVKIEEREGITVYSTQFGGYAKEADYVAHAT 150

Query: 157 KLATSLSRFPLANSTSERGYAYAIAQYDSPFRLIGRVNEVWV 199
           +L T+L   P     + +G  Y  A YD P +  GR NEVW+
Sbjct: 151 QLRTTLEGTP----ATYQGDVYYCAGYDPPMKPYGRRNEVWL 187

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BLAST of Lsi02G003900 vs. ExPASy Swiss-Prot
Match: Q9NRV9 (Heme-binding protein 1 OS=Homo sapiens OX=9606 GN=HEBP1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.9e-09
Identity = 41/165 (24.85%), Postives = 72/165 (43.64%), Query Frame = 0


Query: 34  ESDFEIRFYRKSTWMSAPVQDPSFEKATKNGFHRLFQYIQGANLNWSRIAMTAPVLISII 93
           E  +E R      + +  V D   ++A +    ++ +Y  G N     + MT P+  ++ 
Sbjct: 28  EVAYEERACEGGKFATVEVTDKPVDEALREAMPKVAKYAGGTNDKGIGMGMTVPISFAVF 87

Query: 94  PGAGPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIK 153
           P           V F +P +FQ+ PP P  +  +K    E   V   +F G++++   + 
Sbjct: 88  PNEDGSLQKKLKVWFRIPNQFQSDPPAP-SDKSVKIEEREGITVYSMQFGGYAKEADYVA 147

Query: 154 EAEKLATSLSRFPLANSTSERGYAYAIAQYDSPFRLIGRVNEVWV 199
           +A +L     R  L  + + RG  Y    YD P +  GR NE+W+
Sbjct: 148 QATRL-----RAALEGTATYRGDIYFCTGYDPPMKPYGRRNEIWL 186

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BLAST of Lsi02G003900 vs. ExPASy TrEMBL
Match: A0A0A0LU51 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046240 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 7.9e-115
Identity = 206/216 (95.37%), Postives = 211/216 (97.69%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL LCIAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQDPSFEKA
Sbjct: 1   MVNPKLSIFLLNLSCFLSLCIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQDPSFEKA 60

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 120

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 121 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 181 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 216

[Close]

BLAST of Lsi02G003900 vs. ExPASy TrEMBL
Match: A0A5A7UJA8 (Heme-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00860 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 6.6e-114
Identity = 204/216 (94.44%), Postives = 211/216 (97.69%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL LCIAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 79  MVNPKLSIFLLNLWCFLSLCIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 138

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQ+SPPL
Sbjct: 139 TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQSSPPL 198

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 199 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 258

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 259 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 294

[Close]

BLAST of Lsi02G003900 vs. ExPASy TrEMBL
Match: A0A5D3BHC0 (Heme-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003790 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.6e-113
Identity = 204/216 (94.44%), Postives = 210/216 (97.22%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL L IAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 26  MVNPKLSIFLLNLWCFLSLYIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 85

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 86  TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 145

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 146 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 205

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 206 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 241

[Close]

BLAST of Lsi02G003900 vs. ExPASy TrEMBL
Match: A0A1S3AXL1 (heme-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103483699 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.6e-113
Identity = 204/216 (94.44%), Postives = 210/216 (97.22%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL L IAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 66  MVNPKLSIFLLNLWCFLSLYIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 125

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 126 TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 185

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 186 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 245

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 246 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 281

[Close]

BLAST of Lsi02G003900 vs. ExPASy TrEMBL
Match: A0A6J1KGK1 (heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111495064 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.9e-110
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           MENPKLSIFLVNL C L LCIAIESPQY VVH+ESDFEIRFYRKSTWMSAPVQD SFEKA
Sbjct: 21  MENPKLSIFLVNLFCILSLCIAIESPQYDVVHTESDFEIRFYRKSTWMSAPVQDLSFEKA 80

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TK+GFHRLFQYIQGANLNWSRIAMTAP L SI+PG GPLHSSSYLVQ YLP+KFQASPPL
Sbjct: 81  TKHGFHRLFQYIQGANLNWSRIAMTAPFLTSIVPGTGPLHSSSYLVQLYLPVKFQASPPL 140

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPCGWEAHCVAVRKFSGF+RD KVIKEAE+LATSLSRFPL N+TSERGYAYAI
Sbjct: 141 PLPELKLKPCGWEAHCVAVRKFSGFARDGKVIKEAEQLATSLSRFPLVNATSERGYAYAI 200

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDV+AADLDGCKSSAIAAF
Sbjct: 201 AQYDSPFRLIGRVNEVWVDVNAADLDGCKSSAIAAF 236

[Close]

BLAST of Lsi02G003900 vs. NCBI nr
Match: XP_004152484.1 (heme-binding protein 2 [Cucumis sativus] >KGN64327.1 hypothetical protein Csa_013152 [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 1.6e-114
Identity = 206/216 (95.37%), Postives = 211/216 (97.69%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL LCIAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQDPSFEKA
Sbjct: 1   MVNPKLSIFLLNLSCFLSLCIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQDPSFEKA 60

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 120

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 121 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 181 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 216

[Close]

BLAST of Lsi02G003900 vs. NCBI nr
Match: KAA0055244.1 (heme-binding protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 419.9 bits (1078), Expect = 1.4e-113
Identity = 204/216 (94.44%), Postives = 211/216 (97.69%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL LCIAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 79  MVNPKLSIFLLNLWCFLSLCIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 138

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQ+SPPL
Sbjct: 139 TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQSSPPL 198

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 199 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 258

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 259 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 294

[Close]

BLAST of Lsi02G003900 vs. NCBI nr
Match: XP_038894715.1 (heme-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 418.3 bits (1074), Expect = 4.0e-113
Identity = 205/215 (95.35%), Postives = 210/215 (97.67%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           MENPKLSIFL+NLLCFL LCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA
Sbjct: 1   MENPKLSIFLINLLCFLGLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SIIPGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKP  WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFP+ANSTSERGYAYAI
Sbjct: 121 PLPELKLKPSRWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPMANSTSERGYAYAI 180

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAA 216
           AQYDSPFRLIGRVNEVWVDV A++LDGCKSSAIAA
Sbjct: 181 AQYDSPFRLIGRVNEVWVDVHASELDGCKSSAIAA 215

[Close]

BLAST of Lsi02G003900 vs. NCBI nr
Match: TYJ99170.1 (heme-binding protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 204/216 (94.44%), Postives = 210/216 (97.22%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL L IAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 26  MVNPKLSIFLLNLWCFLSLYIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 85

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 86  TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 145

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 146 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 205

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 206 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 241

[Close]

BLAST of Lsi02G003900 vs. NCBI nr
Match: XP_008438659.1 (PREDICTED: heme-binding protein 2-like [Cucumis melo])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 204/216 (94.44%), Postives = 210/216 (97.22%), Query Frame = 0


Query: 1   MENPKLSIFLVNLLCFLCLCIAIESPQYAVVHSESDFEIRFYRKSTWMSAPVQDPSFEKA 60
           M NPKLSIFL+NL CFL L IAIESPQYAVVHSESDFEIRFYRKSTW+SAPVQ+PSFEKA
Sbjct: 66  MVNPKLSIFLLNLWCFLSLYIAIESPQYAVVHSESDFEIRFYRKSTWISAPVQNPSFEKA 125

Query: 61  TKNGFHRLFQYIQGANLNWSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPL 120
           TKNGFHRLFQYIQGANLNWSRIAMTAPVL SI+PGAGPLHSSSYLVQFYLPLKFQASPPL
Sbjct: 126 TKNGFHRLFQYIQGANLNWSRIAMTAPVLTSIVPGAGPLHSSSYLVQFYLPLKFQASPPL 185

Query: 121 PLPELKLKPCGWEAHCVAVRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 180
           PLPELKLKPC WEAHCVAVRKFSGF+RDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI
Sbjct: 186 PLPELKLKPCDWEAHCVAVRKFSGFARDEKVIKEAEKLATSLSRFPLANSTSERGYAYAI 245

Query: 181 AQYDSPFRLIGRVNEVWVDVDAADLDGCKSSAIAAF 217
           AQYDSPFRLIGRVNEVWVDVDAADL+GCKSSAIAAF
Sbjct: 246 AQYDSPFRLIGRVNEVWVDVDAADLEGCKSSAIAAF 281

[Close]

BLAST of Lsi02G003900 vs. TAIR 10
Match: AT1G17100.1 (SOUL heme-binding family protein )

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-33
Identity = 78/188 (41.49%), Postives = 105/188 (55.85%), Query Frame = 0


Query: 20  CIAIESPQYAVVHSESDFEIRFYRKSTWMSA-PVQDPSFEKATKNGFHRLFQYIQGANLN 79
           C  IE P Y +VHS + +EIR Y  + W+S  P+ D S   AT+  F +LF YIQG N  
Sbjct: 42  CNRIECPSYELVHSGNGYEIRRYNNTVWVSTEPIPDISLVDATRTAFFQLFAYIQGKNEY 101

Query: 80  WSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVA 139
             +I MTAPV+  + P  GP   SS+ V FY+P K Q   P P P   L    W +  VA
Sbjct: 102 HQKIEMTAPVISQVSPSDGPFCESSFTVSFYVPKKNQ---PDPAPSENLHIQKWNSRYVA 161

Query: 140 VRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSE--------RGYAYAIAQYDSPFRLI 199
           VR+FSGF  D+ + ++A  L +SL     AN+ ++           AY +AQY+SPF   
Sbjct: 162 VRQFSGFVSDDSIGEQAAALDSSLKGTAWANAIAKSKEDGGVGSDSAYTVAQYNSPFEFS 221

[Close]

BLAST of Lsi02G003900 vs. TAIR 10
Match: AT1G78450.1 (SOUL heme-binding family protein )

HSP 1 Score: 105.5 bits (262), Expect = 5.3e-23
Identity = 61/194 (31.44%), Postives = 98/194 (50.52%), Query Frame = 0


Query: 20  CIAIESPQYAVVHSESDFEIRFYRKSTWMSA-PVQDPSFEKATKNGFHRLFQYIQGANLN 79
           C   E P Y VVH+ + +EI  Y  + W+S  P+QD S  +A+ NG+++L  Y+ G N  
Sbjct: 31  CNRAECPSYEVVHAGNGYEIHRYNTTVWISTEPIQDISLNEASGNGWNQLSDYMNGNNDY 90

Query: 80  WSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVA 139
             RI +  P +  +       + S+++V F++P  FQ  PP   P   L    W++  VA
Sbjct: 91  HQRIEIALPYITQV-----SQNLSTFIVSFFVPKAFQPDPP---PGNNLHVQRWDSRYVA 150

Query: 140 VRKFSGFSRDEKVIKEAEKLATSLSRFPLANSTSE--------RGYAYAIAQYDSPFRLI 199
           V++ SG+  D K+ K+  +L  SL     A +  +          +AY +AQ+  PF+  
Sbjct: 151 VKQISGYVADHKIGKQVAELKASLQGTVWAKAIEKSRETGGVGSAWAYTVAQFSWPFQWS 210

Query: 200 GRVNEVWVDVDAAD 205
            RVNE+W   +  D
Sbjct: 211 QRVNEIWFPFEMED 216

[Close]

BLAST of Lsi02G003900 vs. TAIR 10
Match: AT1G78460.1 (SOUL heme-binding family protein )

HSP 1 Score: 85.9 bits (211), Expect = 4.4e-17
Identity = 65/185 (35.14%), Postives = 91/185 (49.19%), Query Frame = 0


Query: 20  CIAIESPQYAVVHSESDFEIRFYRKSTWMS-APVQDPSFEKATKNGFHRLFQYIQGANLN 79
           C   E P Y +V +   FEIR Y  + W+S +P+   S  +ATK GF RL +YI+G N +
Sbjct: 40  CDHYECPTYKLVEAGYGFEIRMYDAALWISTSPIPSLSMTQATKTGFRRLNRYIEGDNKS 99

Query: 80  WSRIAMTAPVLISIIPGAGPLHSSSYLVQFYLPLKFQASPPLPLPELKLKPCGWEAHCVA 139
             ++ MTAPV+    PG      S Y V  YLP K Q +PP    +L ++    +   VA
Sbjct: 100 NVKMNMTAPVIAQATPG-----RSVYTVSLYLPKKNQQNPP-QADDLHVR--STKPTYVA 159

Query: 140 VRKFSGFSRDEKVIKEAEKLATSLSR----FPLANSTSERGYAYAIAQYDSPFRLIGRV- 199
           VR+  G+  +     EA  L  SL       P+  S  +   AY +A Y+ P     RV 
Sbjct: 160 VRQIGGYVSNNVAKDEAAALMESLRDSNWILPIEKSKGKLP-AYFLAVYNPPSHTTARVI 215

The following BLAST results are available for this feature:

BLAST of Lsi02G003900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NU05	1.1e-12	29.19	Heme-binding protein 1 OS=Xenopus laevis OX=8355 GN=hebp1 PE=2 SV=1	[more]
Q9R257	2.1e-11	27.78	Heme-binding protein 1 OS=Mus musculus OX=10090 GN=Hebp1 PE=1 SV=2	[more]
Q9NRV9	1.9e-09	24.85	Heme-binding protein 1 OS=Homo sapiens OX=9606 GN=HEBP1 PE=1 SV=1	[more]

BLAST of Lsi02G003900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LU51	7.9e-115	95.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046240 PE=3 SV=1	[more]
A0A5A7UJA8	6.6e-114	94.44	Heme-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A5D3BHC0	5.6e-113	94.44	Heme-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AXL1	5.6e-113	94.44	heme-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103483699 PE=3 SV=1	[more]
A0A6J1KGK1	9.9e-110	91.20	heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111495064 PE=3 SV=...	[more]

BLAST of Lsi02G003900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152484.1	1.6e-114	95.37	heme-binding protein 2 [Cucumis sativus] >KGN64327.1 hypothetical protein Csa_01...	[more]
KAA0055244.1	1.4e-113	94.44	heme-binding protein 2-like [Cucumis melo var. makuwa]	[more]
XP_038894715.1	4.0e-113	95.35	heme-binding protein 2-like [Benincasa hispida]	[more]
TYJ99170.1	1.2e-112	94.44	heme-binding protein 2-like [Cucumis melo var. makuwa]	[more]
XP_008438659.1	1.2e-112	94.44	PREDICTED: heme-binding protein 2-like [Cucumis melo]	[more]

BLAST of Lsi02G003900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17100.1	2.0e-33	41.49	SOUL heme-binding family protein	[more]
AT1G78450.1	5.3e-23	31.44	SOUL heme-binding family protein	[more]
AT1G78460.1	4.4e-17	35.14	SOUL heme-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006917	SOUL haem-binding protein	PFAM	PF04832	SOUL	coord: 23..198 e-value: 3.2E-46 score: 157.6
IPR006917	SOUL haem-binding protein	PANTHER	PTHR11220	HEME-BINDING PROTEIN-RELATED	coord: 11..209
IPR011256	Regulatory factor, effector binding domain superfamily	GENE3D	3.20.80.10	Regulatory factor, effector binding domain	coord: 22..207 e-value: 2.2E-51 score: 176.2
IPR011256	Regulatory factor, effector binding domain superfamily	SUPERFAMILY	55136	Probable bacterial effector-binding domain	coord: 13..200
None	No IPR available	PANTHER	PTHR11220:SF36	SOUL HEME-BINDING PROTEIN-RELATED	coord: 11..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G003900.1	Lsi02G003900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane