Lsi02G002790 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G002790
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	kunitz trypsin inhibitor 2
Location	chr02: 2360483 .. 2361100 (-)
RNA-Seq Expression	Lsi02G002790
Synteny	Lsi02G002790

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

mRNA sequence

ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Coding sequence (CDS)

ATGAAGAGCTTCGGAATATTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTACCGAGGTTCGCTTCTCCAGAGCCGAAGCTTCGCCGGAGGCCGTCCGCGATATCGACGGCAATAAGCTCCGAGCCGGTGTCAACTATTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGAAATGTCCACTGAACGTGGTTCAAGAACAATTCGAATTAATGAACGGCTTGCCTACAACATTTGCGCCTGTAAACCCTAAAAAAGGAGTGATTCGAGTTTCGACGGATTTGAATGTGCAATTCGAGGCGAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGGACGGTAAGGATTATAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

[Close]

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-58
Identity = 108/198 (54.55%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 8   LSFLFILLA-STEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           L ++F+LLA     R    EA+ E V+DI+G  L  GVNYYILPV RGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           E CP +V+Q+QFE+  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 209.1 bits (531), Expect = 4.6e-53
Identity = 113/216 (52.31%), Postives = 140/216 (64.81%), Query Frame = 0


Query: 1   MKSFGIL-LSFLFI---LLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRG 60
           MK   +L LSF F+   L A+     S A+++P  V DIDG KLR G NYYI+PV R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTLGNLQSE---KCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTI 120
           GGLT+          CP  VVQ + E+ +  P  F P NPK+ V+RVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK--DGKDYKLVFCPSV 180
           C   +STVW+LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDIGIFF-KNGKRALALSDTPFPVMFKK 205
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.6e-42
Identity = 94/204 (46.08%), Postives = 122/204 (59.80%), Query Frame = 0


Query: 6   ILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGR-GGGLTLGNL 65
           +LL F F    S    F  A A+   V D DG++L+ GV YY+L    G  GGGL LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 66  QSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFE--ASTICATSTVWKL 125
             + CP  VVQ + +L NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 126 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDG-KDYKLVFCPSVCDFCKVMCRDIG 185
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 186 IFF-KNGKRALALSDTPFPVMFKK 205
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.3e-36
Identity = 87/196 (44.39%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 12  FILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPL 71
           +++LA T V  S A     AV DIDGN +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  E+  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKDGKD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot
Match: P80691 (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.0e-25
Identity = 71/183 (38.80%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 28  SPEAVRDIDGNKLRAGVNYYILPVFRGR-GGGLTL-GNLQSEKCPLNVVQEQFELMNGLP 87
           +P+ + DIDG  +  GV+Y+++    G  GGGLT+ G    +KCPL+VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 88  TTFAPV-NPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPG 147
             F+ + N K  ++  S DLNV+F  +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 148 VETVDNWFKIEKDGK--DYKLVFCPSVCDFCKVMCRDIGIFF-KNGKRALALSDTPFPVM 205
            E+  + FKI+K G    YK  FCPS      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.2e-103
Identity = 185/206 (89.81%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTE-VRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGL 60
           MK+FGIL  FLFILLAST+ +RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSEKCPLNVVQEQ E+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 6.5e-103
Identity = 184/204 (90.20%), Postives = 193/204 (94.61%), Query Frame = 0


Query: 2   KSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTL 61
           K+FGI   F+FILLASTE+RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFELMNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 6.5e-103
Identity = 184/204 (90.20%), Postives = 193/204 (94.61%), Query Frame = 0


Query: 2   KSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTL 61
           K+FGI   F+FILLASTE+RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFELMNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.8e-100
Identity = 180/205 (87.80%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+F  LLSFLFILLAS EVR SRA+ASP+AVRDIDG KLRAGVNYYILPVFRGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+KCPLNVVQEQFELMNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEK G DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.8e-100
Identity = 180/205 (87.80%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+F  LLSFLFILLAS EVR SRA+ASP+AVRDIDG KLRAGVNYYILPVFRGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+KCPLNVVQEQFELMNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEK G DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Lsi02G002790 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 399.1 bits (1024), Expect = 2.4e-107
Identity = 192/205 (93.66%), Postives = 196/205 (95.61%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+FGILLSFLFILLASTEVRFSRA+ASPEAVRDIDG KLRAGVNYYILPV RGRGGGLT
Sbjct: 1   MKNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           L NLQSE CP+NVVQEQFELMNG PTTF PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LSNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KLDK DESTGQWFVTIGGSRGNPGVETVDNWFKI K GKDYKLVFCPSVCDFCKVMCRDI
Sbjct: 121 KLDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Lsi02G002790 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 384.8 bits (987), Expect = 4.6e-103
Identity = 185/206 (89.81%), Postives = 194/206 (94.17%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTE-VRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGL 60
           MK+FGIL  FLFILLAST+ +RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 120
           TLGNLQSEKCPLNVVQEQ E+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDTPFPVMFKKV 206
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of Lsi02G002790 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 184/204 (90.20%), Postives = 193/204 (94.61%), Query Frame = 0


Query: 2   KSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTL 61
           K+FGI   F+FILLASTE+RFS A+ASPEAV DIDG KLRAGVNYYILPVFRGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 62  GNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 121
           GNLQSE CP+NVVQEQFELMNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 122 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIG 181
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEK GKDYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 182 IFFKNGKRALALSDTPFPVMFKKV 206
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Lsi02G002790 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.9 bits (956), Expect = 1.8e-99
Identity = 180/205 (87.80%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+F  LLSFLFILLAS EVR SRA+ASP+AVRDIDG KLRAGVNYYILPVFRGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+KCPLNVVQEQFELMNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEK G DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Lsi02G002790 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.7 bits (953), Expect = 4.1e-99
Identity = 180/205 (87.80%), Postives = 190/205 (92.68%), Query Frame = 0


Query: 1   MKSFGILLSFLFILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLT 60
           MK+F  LLSFLFILLAS EVR SRA+ASP+AVRDIDG KLRAGVNYYILPVFRGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 61  LGNLQSEKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 120
           LGNLQS+KCPLNVVQEQFELMNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDI 180
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEK G DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKKV 206
           GIFFKNGKRALALSDTPFPVMFK V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKVV 203

[Close]

BLAST of Lsi02G002790 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 227.6 bits (579), Expect = 8.8e-60
Identity = 108/198 (54.55%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 8   LSFLFILLA-STEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQS 67
           L ++F+LLA     R    EA+ E V+DI+G  L  GVNYYILPV RGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 68  EKCPLNVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 127
           E CP +V+Q+QFE+  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 128 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCRDIGIFFKN 187
           E+T QWF++  G  GNPG +TVDNWFKI+K  KDYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 188 GKRALALSDTPFPVMFKK 205
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Lsi02G002790 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 154.5 bits (389), Expect = 9.5e-38
Identity = 87/196 (44.39%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 12  FILLASTEVRFSRAEASPEAVRDIDGNKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPL 71
           +++LA T V  S A     AV DIDGN +    +YY+LPV RGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 72  NVVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 131
           ++VQE  E+  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 132 QWFVTIGGSRGNPGVETVDNWFKIEKDGKD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 191
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 192 RALALSDTPFPVMFKK 205
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Lsi02G002790 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 73/208 (35.10%), Postives = 121/208 (58.17%), Query Frame = 0


Query: 6   ILLSFLFI-LLASTEVRFSRAEASP-EAVRDIDGNKLRAGVNYYILPVFRGRGGGL--TL 65
           + LSF+ + +L++     S A+A+P + V DI G+ +++ V YYI+P   G GGGL  + 
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 66  GNLQSEKCPLN--VVQEQFELMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 125
            NL ++   LN  +VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 126 VWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKDGKDYKLVFCPSVCDFCKVMCR 185
           VW++D       + FV+IGG +G       ++WF+I++DG  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 186 DIGI-FFKNGKRALALS-DTPFPVMFKK 205
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Lsi02G002790 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	1.2e-58	54.55	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	4.6e-53	52.31	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	3.6e-42	46.08	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.3e-36	44.39	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P80691	4.0e-25	38.80	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of Lsi02G002790 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTW2	2.2e-103	89.81	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]
A0A5D3BIG0	6.5e-103	90.20	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	6.5e-103	90.20	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A6J1KRM6	8.8e-100	87.80	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	8.8e-100	87.80	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]

BLAST of Lsi02G002790 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895203.1	2.4e-107	93.66	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
XP_004137534.1	4.6e-103	89.81	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]
XP_008437058.1	1.4e-102	90.20	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
XP_022923703.1	1.8e-99	87.80	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	4.1e-99	87.80	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G002790 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	8.8e-60	54.55	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	9.5e-38	44.39	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.3e-23	35.10	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 31..60 score: 43.53 coord: 69..89 score: 33.52 coord: 174..203 score: 25.03 coord: 151..170 score: 25.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..205 e-value: 7.7E-73 score: 258.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..204 e-value: 2.6E-60 score: 203.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 31..204 e-value: 1.29166E-58 score: 180.231
None	No IPR available	GENE3D	2.80.10.50		coord: 26..205 e-value: 7.0E-77 score: 259.1
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G002790.1	Lsi02G002790.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity