Lsi02G001700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G001700
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	peptidyl-tRNA hydrolase, chloroplastic
Location	chr02: 1435782 .. 1436528 (-)
RNA-Seq Expression	Lsi02G001700
Synteny	Lsi02G001700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATTGGTGTTTCTATATCTTCAATCGCCGCCCTGCGCCTTCCTATTTGCCTCCATTGCTTTCGAACCTCCAAAACCCTCATTTCTGTCCCTTCTCTAACCTTCCTTAATTCTGGAACTTCTCATATCTTTTCGTCAATGACTTCTTCCATTACTGCGTCGATGTCTACTTCCTCGTCTCCGACCACCGATCAGCTGCCTGTAGATGACAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTCATCGTGGGCCTCGGGAATCCTGGCAAGAAGTACGATCGTACGCGCCATAATGTCCGTATGGTCCTATCTCTGCTATATGAATTCGAAGTTTCTCTTATCGATCATTTATGTTCTGTTCATTTTTTGCTTTGACCGCAATTGCTTTTCCAGAACTTAGCCTCTGTTTCGTTGATAACTATGTCGAGCGATAGTAGATTATGACTTATTTTCTGTTTGATTAATTAAGTGTGTGTGGGAAACCCCTTGGGGAAGTTTGCCGAGCATGAACTTACGATTATCTCGGGGAAATTAGGGTTTAGTGTTGATTATGAATGTTACCTGGATGTCCTTTATGAAGACGAATTGAAACACTAGCAGTAGCTAATGCTATGACTAATTTTCCAGGTAGGTTTTGAGATGGTGGATACTGTAGCTGAAGCTGAAGGGATATCCATGAGCAGTGTTTCCTTCAAAGCTTTGATCGGGAAAGGTATGCAGTTTTTAACAATTCTTGACAGTTGA

mRNA sequence

ATGAATATTGGTGTTTCTATATCTTCAATCGCCGCCCTGCGCCTTCCTATTTGCCTCCATTGCTTTCGAACCTCCAAAACCCTCATTTCTGTCCCTTCTCTAACCTTCCTTAATTCTGGAACTTCTCATATCTTTTCGTCAATGACTTCTTCCATTACTGCGTCGATGTCTACTTCCTCGTCTCCGACCACCGATCAGCTGCCTGTAGATGACAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTCATCGTGGGCCTCGGGAATCCTGGCAAGAAGTACGATCGTACGCGCCATAATGTCCGTATGGTAGGTTTTGAGATGGTGGATACTGTAGCTGAAGCTGAAGGGATATCCATGAGCAGTGTTTCCTTCAAAGCTTTGATCGGGAAAGGTATGCAGTTTTTAACAATTCTTGACAGTTGA

Coding sequence (CDS)

ATGAATATTGGTGTTTCTATATCTTCAATCGCCGCCCTGCGCCTTCCTATTTGCCTCCATTGCTTTCGAACCTCCAAAACCCTCATTTCTGTCCCTTCTCTAACCTTCCTTAATTCTGGAACTTCTCATATCTTTTCGTCAATGACTTCTTCCATTACTGCGTCGATGTCTACTTCCTCGTCTCCGACCACCGATCAGCTGCCTGTAGATGACAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTCATCGTGGGCCTCGGGAATCCTGGCAAGAAGTACGATCGTACGCGCCATAATGTCCGTATGGTAGGTTTTGAGATGGTGGATACTGTAGCTGAAGCTGAAGGGATATCCATGAGCAGTGTTTCCTTCAAAGCTTTGATCGGGAAAGGTATGCAGTTTTTAACAATTCTTGACAGTTGA

Protein sequence

MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSSSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKGMQFLTILDS

Homology

[Close]

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot
Match: Q8GW64 (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 6.8e-16
Identity = 64/139 (46.04%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 8   SSIAALRLP--ICLHCFRTSKTLIS----VPSLTFLNSGTSHIFSSMTSSITASMSTSSS 67
           + IA L  P   C   FR+  T +S       LT    G   + S   SS + S+S SS+
Sbjct: 8   AKIANLSFPSNCCSLFFRSPATFLSPALPCRKLTKGIRGLEGLMSQCLSSSSQSLSFSSN 67

Query: 68  PTTDQ--------LPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAE 127
             + Q        LP    K  P   PWLIVGLGNPGKKY  TRHN   VGFEMVD +A+
Sbjct: 68  SFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHN---VGFEMVDALAD 127

Query: 128 AEGISMSSVSFKALIGKGM 133
           AEGISM++V+FKAL GKG+
Sbjct: 128 AEGISMNTVNFKALFGKGV 143

[Close]

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot
Match: Q5N9Q7 (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.4e-15
Identity = 38/53 (71.70%), Postives = 44/53 (83.02%), Query Frame = 0


Query: 79  KPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           KPWL VGLGNPGK Y  TRHN   VGFEM+D +AEAEGIS+SS+ FKA++GKG
Sbjct: 54  KPWLFVGLGNPGKVYQGTRHN---VGFEMIDVIAEAEGISLSSMQFKAMVGKG 103

[Close]

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-11
Identity = 33/52 (63.46%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 80  PWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           PWLIVGLGNPG KY  TRHN   VGFEM+D +A  EG+ M+++  KALIG G
Sbjct: 45  PWLIVGLGNPGNKYHGTRHN---VGFEMIDVLARKEGVLMNTIQSKALIGIG 93

[Close]

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot
Match: Q10LI6 (CRS2-like protein, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0347800 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-11
Identity = 32/53 (60.38%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 80  PWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKGM 133
           PWL VGLGNPG+KY  TRHN   VGF+M+D  A+++GIS++   FKAL G+GM
Sbjct: 60  PWLFVGLGNPGEKYQCTRHN---VGFDMIDMFAQSQGISLTRHPFKALFGEGM 109

[Close]

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-11
Identity = 31/53 (58.49%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 79  KPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           +PWL +GLGNPG KY  TRHN   +GFEM+D  AE+ GI M+ V+FKA++G+G
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHN---IGFEMIDVFAESVGIQMNLVNFKAIMGQG 66

[Close]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL
Match: A0A1S3C5R3 (peptidyl-tRNA hydrolase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496915 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.5e-47
Identity = 107/131 (81.68%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNIG SISS+AALRLP  +H FRTS+TLIS PSL+ L+S T H FSS+TSSITASMSTSS
Sbjct: 1   MNIGASISSVAALRLPFSIHSFRTSRTLISFPSLSLLHSPTFHNFSSITSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
           SPTTDQ  +DDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVD VAEAEGIS+S
Sbjct: 61  SPTTDQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDAVAEAEGISIS 120

Query: 121 SVSFKALIGKG 132
           +VSFKALIGKG
Sbjct: 121 NVSFKALIGKG 128

[Close]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL
Match: A0A0A0LVQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042230 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.9e-47
Identity = 107/131 (81.68%), Postives = 115/131 (87.79%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           M IG SISS+AALRLP   HCF TSKTLIS PSL+ L+S T HIFSS+TSSITASMSTSS
Sbjct: 1   MIIGASISSVAALRLPFSFHCFPTSKTLISFPSLSLLHSPTFHIFSSITSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
           SPTT+Q  +DDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVD VAEAEGIS+S
Sbjct: 61  SPTTEQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDAVAEAEGISIS 120

Query: 121 SVSFKALIGKG 132
           +VSFKALIGKG
Sbjct: 121 NVSFKALIGKG 128

[Close]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL
Match: A0A6J1JZW9 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490352 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 6.6e-46
Identity = 107/139 (76.98%), Postives = 117/139 (84.17%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNI  S+SS+AALRLPICLHCF T +T IS PSL+ LN  + HIFSSMTSSITASMSTSS
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLS-LNPPSLHIFSSMTSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
               DQL V++KKPAPPPKPWLIVGLGNPGKKY+RTRHN   VGFEM+D VAE EGISMS
Sbjct: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHN---VGFEMLDAVAEVEGISMS 120

Query: 121 SVSFKALIGKGMQFLTILD 140
           S SFKALIGKG+Q LTILD
Sbjct: 121 SASFKALIGKGVQILTILD 135

[Close]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL
Match: A0A6J1K317 (peptidyl-tRNA hydrolase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.2e-42
Identity = 101/131 (77.10%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNI  S+SS+AALRLPICLHCF T +T IS PSL+ LN  + HIFSSMTSSITASMSTSS
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLS-LNPPSLHIFSSMTSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
               DQL V++KKPAPPPKPWLIVGLGNPGKKY+RTRHN   VGFEM+D VAE EGISMS
Sbjct: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHN---VGFEMLDAVAEVEGISMS 120

Query: 121 SVSFKALIGKG 132
           S SFKALIGKG
Sbjct: 121 SASFKALIGKG 127

[Close]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL
Match: A0A6J1JW15 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.2e-42
Identity = 101/131 (77.10%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNI  S+SS+AALRLPICLHCF T +T IS PSL+ LN  + HIFSSMTSSITASMSTSS
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLS-LNPPSLHIFSSMTSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
               DQL V++KKPAPPPKPWLIVGLGNPGKKY+RTRHN   VGFEM+D VAE EGISMS
Sbjct: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHN---VGFEMLDAVAEVEGISMS 120

Query: 121 SVSFKALIGKG 132
           S SFKALIGKG
Sbjct: 121 SASFKALIGKG 127

[Close]

BLAST of Lsi02G001700 vs. NCBI nr
Match: XP_038893864.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893865.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893866.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 122/132 (92.42%), Postives = 125/132 (94.70%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMT-SSITASMSTS 60
           MN+G SISS+ ALRLPICLHCFRTSKTLIS PSL+FLNSGTSHIFSSMT SSITASMSTS
Sbjct: 1   MNVGSSISSVTALRLPICLHCFRTSKTLISFPSLSFLNSGTSHIFSSMTSSSITASMSTS 60

Query: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISM 120
           SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVDTVAEAEGISM
Sbjct: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDTVAEAEGISM 120

Query: 121 SSVSFKALIGKG 132
           SSVSFKALIGKG
Sbjct: 121 SSVSFKALIGKG 129

[Close]

BLAST of Lsi02G001700 vs. NCBI nr
Match: XP_038893867.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X2 [Benincasa hispida] >XP_038893868.1 peptidyl-tRNA hydrolase, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 122/132 (92.42%), Postives = 125/132 (94.70%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMT-SSITASMSTS 60
           MN+G SISS+ ALRLPICLHCFRTSKTLIS PSL+FLNSGTSHIFSSMT SSITASMSTS
Sbjct: 1   MNVGSSISSVTALRLPICLHCFRTSKTLISFPSLSFLNSGTSHIFSSMTSSSITASMSTS 60

Query: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISM 120
           SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVDTVAEAEGISM
Sbjct: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDTVAEAEGISM 120

Query: 121 SSVSFKALIGKG 132
           SSVSFKALIGKG
Sbjct: 121 SSVSFKALIGKG 129

[Close]

BLAST of Lsi02G001700 vs. NCBI nr
Match: XP_008457243.1 (PREDICTED: peptidyl-tRNA hydrolase, chloroplastic [Cucumis melo])

HSP 1 Score: 197.6 bits (501), Expect = 7.2e-47
Identity = 107/131 (81.68%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNIG SISS+AALRLP  +H FRTS+TLIS PSL+ L+S T H FSS+TSSITASMSTSS
Sbjct: 1   MNIGASISSVAALRLPFSIHSFRTSRTLISFPSLSLLHSPTFHNFSSITSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
           SPTTDQ  +DDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVD VAEAEGIS+S
Sbjct: 61  SPTTDQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDAVAEAEGISIS 120

Query: 121 SVSFKALIGKG 132
           +VSFKALIGKG
Sbjct: 121 NVSFKALIGKG 128

[Close]

BLAST of Lsi02G001700 vs. NCBI nr
Match: XP_004137468.1 (peptidyl-tRNA hydrolase, chloroplastic [Cucumis sativus] >KGN64111.1 hypothetical protein Csa_014269 [Cucumis sativus])

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-46
Identity = 107/131 (81.68%), Postives = 115/131 (87.79%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           M IG SISS+AALRLP   HCF TSKTLIS PSL+ L+S T HIFSS+TSSITASMSTSS
Sbjct: 1   MIIGASISSVAALRLPFSFHCFPTSKTLISFPSLSLLHSPTFHIFSSITSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
           SPTT+Q  +DDKKPAPPPKPWLIVGLGNPGKKYDRTRHN   VGFEMVD VAEAEGIS+S
Sbjct: 61  SPTTEQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHN---VGFEMVDAVAEAEGISIS 120

Query: 121 SVSFKALIGKG 132
           +VSFKALIGKG
Sbjct: 121 NVSFKALIGKG 128

[Close]

BLAST of Lsi02G001700 vs. NCBI nr
Match: XP_022994701.1 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 193.4 bits (490), Expect = 1.4e-45
Identity = 107/139 (76.98%), Postives = 117/139 (84.17%), Query Frame = 0


Query: 1   MNIGVSISSIAALRLPICLHCFRTSKTLISVPSLTFLNSGTSHIFSSMTSSITASMSTSS 60
           MNI  S+SS+AALRLPICLHCF T +T IS PSL+ LN  + HIFSSMTSSITASMSTSS
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLS-LNPPSLHIFSSMTSSITASMSTSS 60

Query: 61  SPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMS 120
               DQL V++KKPAPPPKPWLIVGLGNPGKKY+RTRHN   VGFEM+D VAE EGISMS
Sbjct: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHN---VGFEMLDAVAEVEGISMS 120

Query: 121 SVSFKALIGKGMQFLTILD 140
           S SFKALIGKG+Q LTILD
Sbjct: 121 SASFKALIGKGVQILTILD 135

[Close]

BLAST of Lsi02G001700 vs. TAIR 10
Match: AT1G18440.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 85.1 bits (209), Expect = 4.8e-17
Identity = 64/139 (46.04%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 8   SSIAALRLP--ICLHCFRTSKTLIS----VPSLTFLNSGTSHIFSSMTSSITASMSTSSS 67
           + IA L  P   C   FR+  T +S       LT    G   + S   SS + S+S SS+
Sbjct: 8   AKIANLSFPSNCCSLFFRSPATFLSPALPCRKLTKGIRGLEGLMSQCLSSSSQSLSFSSN 67

Query: 68  PTTDQ--------LPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAE 127
             + Q        LP    K  P   PWLIVGLGNPGKKY  TRHN   VGFEMVD +A+
Sbjct: 68  SFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHN---VGFEMVDALAD 127

Query: 128 AEGISMSSVSFKALIGKGM 133
           AEGISM++V+FKAL GKG+
Sbjct: 128 AEGISMNTVNFKALFGKGV 143

[Close]

BLAST of Lsi02G001700 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-12
Identity = 33/52 (63.46%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 80  PWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           PWLIVGLGNPG KY  TRHN   VGFEM+D +A  EG+ M+++  KALIG G
Sbjct: 45  PWLIVGLGNPGNKYHGTRHN---VGFEMIDVLARKEGVLMNTIQSKALIGIG 93

[Close]

BLAST of Lsi02G001700 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-12
Identity = 31/53 (58.49%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 79  KPWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           +PWL +GLGNPG KY  TRHN   +GFEM+D  AE+ GI M+ V+FKA++G+G
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHN---IGFEMIDVFAESVGIQMNLVNFKAIMGQG 66

[Close]

BLAST of Lsi02G001700 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-12
Identity = 33/52 (63.46%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 80  PWLIVGLGNPGKKYDRTRHNVRMVGFEMVDTVAEAEGISMSSVSFKALIGKG 132
           PWLIVGLGNPG KY  TRHN   VGFEM+D +A  EG+ M+++  KALIG G
Sbjct: 45  PWLIVGLGNPGNKYHGTRHN---VGFEMIDVLARKEGVLMNTIQSKALIGIG 93

[Close]

BLAST of Lsi02G001700 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-11
Identity = 42/82 (51.22%), Postives = 49/82 (59.76%), Query Frame = 0


Query: 51  SITASMSTSSSPTTDQLPVDDKKPAP-PPKPWLIVGLGNPGKKYDRTRHNVRMVGFEMVD 110
           S T S S   S     LPV D K       PWLIVGLGNPG KY  TRHN   +GFEM+D
Sbjct: 25  SQTKSNSKRFSSLRASLPVSDNKLLKFEYTPWLIVGLGNPGLKYYGTRHN---IGFEMID 84

Query: 111 TVAEAEGISMSSVSFKALIGKG 132
            +A A  ISM+++  KAL+G G
Sbjct: 85  HIARATDISMNTIQSKALVGIG 103

The following BLAST results are available for this feature:

BLAST of Lsi02G001700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GW64	6.8e-16	46.04	Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18...	[more]
Q5N9Q7	3.4e-15	71.70	Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LF14	2.3e-11	63.46	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]
Q10LI6	2.3e-11	60.38	CRS2-like protein, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q6NLS8	2.3e-11	58.49	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19...	[more]

BLAST of Lsi02G001700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C5R3	3.5e-47	81.68	peptidyl-tRNA hydrolase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496915 P...	[more]
A0A0A0LVQ7	5.9e-47	81.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042230 PE=3 SV=1	[more]
A0A6J1JZW9	6.6e-46	76.98	peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1K317	1.2e-42	77.10	peptidyl-tRNA hydrolase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1JW15	1.2e-42	77.10	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of Lsi02G001700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893864.1	1.1e-55	92.42	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893...	[more]
XP_038893867.1	1.1e-55	92.42	peptidyl-tRNA hydrolase, mitochondrial isoform X2 [Benincasa hispida] >XP_038893...	[more]
XP_008457243.1	7.2e-47	81.68	PREDICTED: peptidyl-tRNA hydrolase, chloroplastic [Cucumis melo]	[more]
XP_004137468.1	1.2e-46	81.68	peptidyl-tRNA hydrolase, chloroplastic [Cucumis sativus] >KGN64111.1 hypothetica...	[more]
XP_022994701.1	1.4e-45	76.98	peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lsi02G001700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18440.1	4.8e-17	46.04	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.1	1.6e-12	63.46	Peptidyl-tRNA hydrolase family protein	[more]
AT5G19830.1	1.6e-12	58.49	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.2	1.6e-12	63.46	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	4.0e-11	51.22	Peptidyl-tRNA hydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	3.40.50.1470		coord: 69..137 e-value: 1.8E-14 score: 55.5
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 80..131
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 82..131 e-value: 5.3E-11 score: 42.7
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 57..132
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 54..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G001700.1	Lsi02G001700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity