Lsi02G001070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G001070
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDiacylglycerol kinase
Locationchr02: 870509 .. 877383 (-)
RNA-Seq ExpressionLsi02G001070
SyntenyLsi02G001070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAATTAAAGAAAAAAGGAGGAAAAAAAAGAGGGAACGTGACTTCCATCTGAAAATTCTCAAATCCGTACCTTTCTCCCGACATCGAAAGCTTCGGATTTGGGTCTTGCCACGATTCCTTCGATTCAAGGAATACCGTGATTATACTATTTGAAGATCGAGTTAGGGTTTTCTGTGATCGGAGCATTTGATCCTGTTATTCACTCATTGTTTTTTTCAGTAAGTTTAATTCATCCATCAGGTTCTCGTGTGCCCCATTTTCACCGTTCTTCGTGGTTATGCTTGTAGTTTCTAAAATGAATTTGCTCCGTACATTATTTGGGTATTGAGAGGAAGATTAGAACGTGTTTAGTGTGGTATGAACTTGAATATTTTGTGCTGCTGGTTTTTTTTTTGCTATGATTCTTAGTTGCTAAGCGCCTTATTGGGATTGTTACAATCTCTATTGAGGTGTTCTTTTGCTTATTATTTATATATTCTTTTATTGAATTAATCGTCGTAAATCTTGTTAAATGTATCCTTTAGAATTTAATATGACCCCAGCATTCATGATATTCTTAGGAATACATTGGAATTTCAACGTTAGAGGATAGCTTTGAAGATTTTTGTTGTTCATTGTCTTTTTTAATGGTGGCGTTCAAACTTTGAAGTTTCATTTTTAATGTTTACTATTTCACATTTTCAGGAGTTATGTGGATTGTCTGTCAATATTTTATTTCACCCTTTGGAAGTGTGGTGGGGGTTTACATCCGTGATATTGAGATGTGAAATAGTGAGTTACCACCTATAAATTGGTAATGTCATCATTAAGTTGGACTCAATTATTAAAAATTGGTTTTGCTTTGGATTACGTTGGAGTTTGGGCGGTAATTGGCTGTCAACTGTCTTGCGCCAAATTTCTTGTTTGAGCATTTGATGTTTCTATTTTCATATCCGTGATTAAAAAGATGGATGAAGACTGGGAAATTTTGTCCCCAAGTTGGAACAGCAAGAATCCAACTGATCGCCTTTTTGTGATTTCTTGCTTTTGTGCTGCCATCATTGGAATTTTGACCATAGCCTTTACGGCCTTCCAATGGCGGAGAAATATTAATTTGAGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAAACTCATAGAGTTCCTGTAGCTGCACACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAGTTGTTGTGTGTGCTTAAAGTTTGTGTCCCCTTCACAGACTCTTGGTCCTATGGTCGCGTCAGATAGTTTTATTCACCGTTGCAACATCTGTGGTGTGGCAGCTCACCTGAGCTGTTCATCTAATGCTCAAAAGGATTGCAAGTGTGTATCTATGATCGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGATCAACCTGATGAAACCTCTTTCTGCAGTTACTGTGAAGAACCATGTAGTGGATCTTTTCTTGGTGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTTTAGTGCATGTTGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGACTTAGGTTCATTTAGAAGGTTGATTCTGTCACCACTTTATGTTAAGGAGTCAAATAGGACGTCCTCTGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCCTCAGTGCGTGCAAGTATAAGGAATCAGAGTAAGAAGAACAAACATAGTCGTAAACCGTCTATCCATTCAGGTCCAAGTGGTAGTATTCGTGATATGTCTACAGAAAGCACAGCTGATTCTCATCACGCATCTAACGGTTATCATGGAACTGAAAGAAACTGTAATGGTAGCAGAACTTCAGAGGGGCCGCATCAAAATGGTGACATAGATGATAAAAGTATTTCAAATCCAAGCTTAAAAAAAAATTCCACACTCAATCAAAAGGATGAAACTCATATATTAGGAATGAACCTGAGATATGAGGTGATTGAAATGCCGTCAGATTCACGTCCCCTGTTAGTATTTATCAACAAGAAAAGTGGGGCCCGCCGTGGAGATTCTTTAAAACAACGGTTAAACATGCTTTTGAATCCTGTGCAGGTATGTGTTTTTCTCCATTCTATTCAAAACAGGCTTTAACTTTAGAAATAGACTGTTGTATTCTACAGCCTAGAATAATTTGTTCAACCTGCTCTGTACTCTTTGTTTGATACCTGGTGATCAATGAGGCAGACAGCAATTTTTTTTGTATAGCTTTTGCACAACTTCCCTTTCTTTATGGAATTCGGTGGGAACTTGGAGCATATAAGTCTGGGTCTATCGAAAATACAATATTGAGGTCAATGAAACCCTCTAAAAGTAACTGATTTATTTATTTTTGAACTTGGCGTTGTAAATGTTTGTTATGAAAAAAGAAAAAAAATGCTTTATATGACTAGTAGGGTTAAACCTCATTTTCTTTGGAATCTATTCACTGCAAGTGTTAATGATGTTTATGAGGTAATCATCGTCAACTCTCTGGTGCTAATATTTCAGGTTTTTGAGTTGAGTTCTACACAAGGACCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCGCACTTCAAGGTTCTTGTATGTGGGGGCGACGGAACTGTTGGTTGGGTCTTAAACTGTATAGACAAGCAAAATTTTGTTTCACCTCCCCCAGTTGCTATTCTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGTACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCAATAGTGGATCAACATGGAAAGCAACTCAAATCTCCACAATTTATGAATAACTATCTAGGTAAGTTTTATTGATATACGTGTGTGTGTGTGTGTATTTTGTGTATTTAAGGTATTGGATAATAAATGCAGCCAAACTTCAGGGCACATCTGAAACATATATCTCATCTTCTGGGTTCTTCTATTGAACCCTTGTTGCATCTGTCTTTTTTTTCTACTTTCTTTTGTTTTGTTTATATTTTACTGCATCATTTCCAATTTGTCGCTCAAAATGCAGGAATTGGATGTGATGCTAAGGTTGCCCTTGACATACATAATTTACGGGAGGAAAATCCAGAAAAATTTTATAACCAGGTGATAAAAGTTGTAGACTTAAAAGATAACTTTAACTTCCTCGAAAATAATCCATCACATAAAGTCATGCTGACTGTCATTGAAACCATGCATGACTGCTATTACAACTTTTCAAACTGTATGTTTATTATTCGAGAACAGTGTAGTTATGTTATATCCTTGTTTCTTATGTCATTAGACATTAAATTTTTTCTCAGGGTTGTGCCTTACTTCTTTTATTCCCAAAATGTGGATAATTTTATGAGCATTTATTTTTATGTTGAATGGCTGAACTCTTTGTTGCCAAAAAAAACAACGTTGTAATGAAGGGTGGTCATATTATGGAACCTGAGAGGATTTAATACTGCATGGTAAAGATCTTAATACAATACTTGCCTCTAGTACTAAGTTGAATGCATATTGATATAGTCCAATAGAAGTGTTCATTTTATTCAATGCATAAGTTTTTTTCATTTCTTAACTTGATTTCTCCTTTCTTAAAAGAAATCAAAACGTATTAGTTACATATACGTTATAAAAGTATATGTCTATATTTGCAGAAGTTCTTGCTAGTTGTTGCTTATTGGTTGTCTCTGATCTTAGGCTATTTGAGTAGTTCATATCACTTAGCTGAAACTATTAACATAACTGCAGTTTATGAACAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGATAGGACATTCGCAGACATTCCATGGCAAGTTCGTGTGGAAGTGGATGGTGCTGAGGTAGAGGTTCCTGAGGTAAGAACTTAACTTTGAAGCCTTTGGTTCTGAATTATTACTAAATTGTCACTCTATTTTCTATTTTATGATTTAGAAGTTTTAGGCTAATATTCAATTACTCAATAGCATAATGATACATTTTAATTTTTGGGTGTGAATTCTTGGAGATTTTTCGTTTATCAAGCATTGATGCATACTATTTGTGTTGTTTATTTGTGACGACTTTGTCCTGAATTATCATTTACTTGAGGTCGATCATTTGAAACTTTAATTTGAATATTTACAGGATGCAGAAGGTGTGCTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTTTGGCATAACGAGGACGAGACTTTTGATAATTTTGATTCACAGTCCATGCATGATAAACTACTCGAGGTTGTCAGCATATCTGGGACTTGGCACCTCGGAAAGTTACAGGTATACAATAATTCAAATAAACGATTCTGTCTCCACATGCTACCAAATCAAATGTTATGGATCCATGTTAGCCTTCATGTGCAATGATGCCTTCTCTAATTTTTTGTTGCTAGGCACTCATAAAAATGGGTTAGCTTTACCACTTTAGTGGGTCTTGATTAAATGTGCGTGTGTTGGAGAAAGAGACTCCCAACAATATTTAAGAGATTCTCTAAAAGAAATATTAGAGGGGTTCAAGCGCAATAAGGCATGCCACTTGCACTAGGGCACGCGGTAGTGCTCTTTAGTTAGAGCCTCCTTGTGGAGCACGTACTCATAACTAAACTGTGGCCTATGTGTGCTTTTCAAAACTGTCTATAAGGTTTATTAAAACTAAAGATATTATTACCCTGGAAAATGGACGGTGAAATCATAAAAGCCTTCTTAGTGTAAGGTCGTGTGTCGATGCTAAATCAAATGTTAGGGACCTCAAACTTAGAGTGACGATGCTGAGTCCTAGTGTATACTAGGAGTCCTAAAACATGAAGTGGTTGGGATCTTGGGTAAAGCGCAAGGTTCATACTTAGAGTTGTGTTGTAACTTCCTTGCTTAGATGTTTGATGGCTGCTGCTTTCTGCTTGAAACAGGTGGGGCTTTCTCGAGCTAGACGACTTGCACAGGGGAAGTCGATTAGGATACAATTATGTGCTGCATTGCCTGTTCAAATTGATGGAGAGCCTTGGTTTCAGGAATTCCCGTGTACACTAGTCATATCCCACCATGGACAGGTAATTGCTCTTGTTCTCTCCTTTCGTTTCTTTTCATTGAAGAAGATCGTTACGCAGCCCATGACCTTGTGGAGTTAGTTTCTTGTGCAGCATCATGCTTAATATCTCTCTCTCTGGGATCACAAAAGAAAAGAAATAAATAATAATAATAATAATAATAATAAAAAATCATGCATGGTATTTCTCTGTTGTTAAGTAACCCCTGTCGTGCTGTGACCTTTGATGCGAAAGCCATAATATAAAATTATAAACAGACAAATAAATAAAGGCATTTAGGCTCAATATATGTTTTAGTCCCTAATGTTTGGGGTGCCTTTTCAATATTGGTTAATAAGTTTGAATTTGGTATTTTACACCCCAAACAATATTGGTTAATAAGTTTGAATTCGGTAGTTTATGTTCTAATAATTTTTAATTGTTACCTTTTCATCGACGAAGGTTAAAACGGCCTAGTAATAAGCCATGATATGATACTTTGTGGTCTCAGGTCTGACAGAAATTTTAAGGAGAAATTAGGTTATTAAGAAAAATTGAATATGCCAACTTAGTGAAGGAATTAGAGAAGTTTTTCACCTGGTAGCCTAGTTTATTCTCTAAATTTATGCCAAGTCACTGAGGACAATGCAACTTAAGCCCTATCATAAGTCAATTTTAGCATTCACCAATTTCATTGGTTATAATTGAAAAATCTTAAAACATAAGATACTATATTGAATTGAATGGGACTAAAATAAAAAAAAAAAAAAAAAAGAAAAAGAAAAAGTCCGGCATTGGGACTAAAACACATATTTAACTTATATATTTATTTCCTTGAAATTGAAAAAGAAGGGAATAGGTTAGAAGACGAGGGAGAATTCATCTATGACAATGCTTCTCCCGAGGATCTGAAATGTGTTCAACTCCGTCACTCTAATCTTTTGGTTTCTAAGGATTGGCTTTGGTGAATATACAAGTTTCCTAGATCTACTTAGCGTTTCTGCTATGAGCAGAAGCTTGTTCTCAAACATCTACTTTTCCATCTAGATTTAGCACCATGTTTCTACTTTCATTTGTTCGGATCTAATACTTGGACGTTGCTTTGCTTTGAAATAGCGTTTTCAAGCTTCATAGTTACTTTCGCTAATCCGTAAAAATACATCCTTAACTAGTTTTCAAAGAAATGATTAGGGGTACTATCTATTTGGCTTCGAGAAAGTCTCACTTCTTGTAGTGTAGGAAACGGGAAATTTCTCTGGCATGATTAGATTTGTTTTATGGGCGTAAGATGTGTTTGATCTGTCTCCCGATAGAAATGTGTGAATTCATGCGGATTTAAATTTTGCTTTATTATAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTTGGTCACGCAGCTGCAATTATCACTGATGTTTTGGAGAGTGCTGAATCTAATAATGTAATTAATGCTTCACAAAAGCGAGTACTTCTCCAAGAAATGGCAAAGAGGTTAACTTGAAAGTTGCTATTAGTCCTTCCTGTAATGGTCAGGGTTTTGAGTACGAATCATTTTCAGCTTTTTATGCCACCCTTCACATCTCTCCATAGAGGTAAGATCTAGTGGTTATTTTTTAAATTTTTTGTTTTGTCTTCCCTTGATGGGTTATAGCCGAGGCATGATTGTATATCAACGATATCTGTTATATAAATATATACATATACGTAGAGTTCCTCAACACGGATCATTATCAA

mRNA sequence

GGAAAATTAAAGAAAAAAGGAGGAAAAAAAAGAGGGAACGTGACTTCCATCTGAAAATTCTCAAATCCGTACCTTTCTCCCGACATCGAAAGCTTCGGATTTGGGTCTTGCCACGATTCCTTCGATTCAAGGAATACCGTGATTATACTATTTGAAGATCGAGTTAGGGTTTTCTGTGATCGGAGCATTTGATCCTGTTATTCACTCATTGTTTTTTTCAGAGTTATGTGGATTGTCTGTCAATATTTTATTTCACCCTTTGGAAGTGTGGTGGGGGTTTACATCCGTGATATTGAGATGTGAAATAGTGAGTTACCACCTATAAATTGGTAATGTCATCATTAAGTTGGACTCAATTATTAAAAATTGGTTTTGCTTTGGATTACGTTGGAGTTTGGGCGGTAATTGGCTGTCAACTGTCTTGCGCCAAATTTCTTGTTTGAGCATTTGATGTTTCTATTTTCATATCCGTGATTAAAAAGATGGATGAAGACTGGGAAATTTTGTCCCCAAGTTGGAACAGCAAGAATCCAACTGATCGCCTTTTTGTGATTTCTTGCTTTTGTGCTGCCATCATTGGAATTTTGACCATAGCCTTTACGGCCTTCCAATGGCGGAGAAATATTAATTTGAGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAAACTCATAGAGTTCCTGTAGCTGCACACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAGTTGTTGTGTGTGCTTAAAGTTTGTGTCCCCTTCACAGACTCTTGGTCCTATGGTCGCGTCAGATAGTTTTATTCACCGTTGCAACATCTGTGGTGTGGCAGCTCACCTGAGCTGTTCATCTAATGCTCAAAAGGATTGCAAGTGTGTATCTATGATCGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGATCAACCTGATGAAACCTCTTTCTGCAGTTACTGTGAAGAACCATGTAGTGGATCTTTTCTTGGTGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTTTAGTGCATGTTGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGACTTAGGTTCATTTAGAAGGTTGATTCTGTCACCACTTTATGTTAAGGAGTCAAATAGGACGTCCTCTGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCCTCAGTGCGTGCAAGTATAAGGAATCAGAGTAAGAAGAACAAACATAGTCGTAAACCGTCTATCCATTCAGGTCCAAGTGGTAGTATTCGTGATATGTCTACAGAAAGCACAGCTGATTCTCATCACGCATCTAACGGTTATCATGGAACTGAAAGAAACTGTAATGGTAGCAGAACTTCAGAGGGGCCGCATCAAAATGGTGACATAGATGATAAAAGTATTTCAAATCCAAGCTTAAAAAAAAATTCCACACTCAATCAAAAGGATGAAACTCATATATTAGGAATGAACCTGAGATATGAGGTGATTGAAATGCCGTCAGATTCACGTCCCCTGTTAGTATTTATCAACAAGAAAAGTGGGGCCCGCCGTGGAGATTCTTTAAAACAACGGTTAAACATGCTTTTGAATCCTGTGCAGGTTTTTGAGTTGAGTTCTACACAAGGACCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCGCACTTCAAGGTTCTTGTATGTGGGGGCGACGGAACTGTTGGTTGGGTCTTAAACTGTATAGACAAGCAAAATTTTGTTTCACCTCCCCCAGTTGCTATTCTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGTACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCAATAGTGGATCAACATGGAAAGCAACTCAAATCTCCACAATTTATGAATAACTATCTAGGAATTGGATGTGATGCTAAGGTTGCCCTTGACATACATAATTTACGGGAGGAAAATCCAGAAAAATTTTATAACCAGTTTATGAACAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGATAGGACATTCGCAGACATTCCATGGCAAGTTCGTGTGGAAGTGGATGGTGCTGAGGTAGAGGTTCCTGAGGATGCAGAAGGTGTGCTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTTTGGCATAACGAGGACGAGACTTTTGATAATTTTGATTCACAGTCCATGCATGATAAACTACTCGAGGTTGTCAGCATATCTGGGACTTGGCACCTCGGAAAGTTACAGGTGGGGCTTTCTCGAGCTAGACGACTTGCACAGGGGAAGTCGATTAGGATACAATTATGTGCTGCATTGCCTGTTCAAATTGATGGAGAGCCTTGGTTTCAGGAATTCCCGTGTACACTAGTCATATCCCACCATGGACAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTTGGTCACGCAGCTGCAATTATCACTGATGTTTTGGAGAGTGCTGAATCTAATAATGTAATTAATGCTTCACAAAAGCGAGTACTTCTCCAAGAAATGGCAAAGAGGTTAACTTGAAAGTTGCTATTAGTCCTTCCTGTAATGGTCAGGGTTTTGAGTACGAATCATTTTCAGCTTTTTATGCCACCCTTCACATCTCTCCATAGAGGTAAGATCTAGTGGTTATTTTTTAAATTTTTTGTTTTGTCTTCCCTTGATGGGTTATAGCCGAGGCATGATTGTATATCAACGATATCTGTTATATAAATATATACATATACGTAGAGTTCCTCAACACGGATCATTATCAA

Coding sequence (CDS)

ATGGATGAAGACTGGGAAATTTTGTCCCCAAGTTGGAACAGCAAGAATCCAACTGATCGCCTTTTTGTGATTTCTTGCTTTTGTGCTGCCATCATTGGAATTTTGACCATAGCCTTTACGGCCTTCCAATGGCGGAGAAATATTAATTTGAGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAAACTCATAGAGTTCCTGTAGCTGCACACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAGTTGTTGTGTGTGCTTAAAGTTTGTGTCCCCTTCACAGACTCTTGGTCCTATGGTCGCGTCAGATAGTTTTATTCACCGTTGCAACATCTGTGGTGTGGCAGCTCACCTGAGCTGTTCATCTAATGCTCAAAAGGATTGCAAGTGTGTATCTATGATCGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGATCAACCTGATGAAACCTCTTTCTGCAGTTACTGTGAAGAACCATGTAGTGGATCTTTTCTTGGTGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTTTAGTGCATGTTGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGACTTAGGTTCATTTAGAAGGTTGATTCTGTCACCACTTTATGTTAAGGAGTCAAATAGGACGTCCTCTGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCCTCAGTGCGTGCAAGTATAAGGAATCAGAGTAAGAAGAACAAACATAGTCGTAAACCGTCTATCCATTCAGGTCCAAGTGGTAGTATTCGTGATATGTCTACAGAAAGCACAGCTGATTCTCATCACGCATCTAACGGTTATCATGGAACTGAAAGAAACTGTAATGGTAGCAGAACTTCAGAGGGGCCGCATCAAAATGGTGACATAGATGATAAAAGTATTTCAAATCCAAGCTTAAAAAAAAATTCCACACTCAATCAAAAGGATGAAACTCATATATTAGGAATGAACCTGAGATATGAGGTGATTGAAATGCCGTCAGATTCACGTCCCCTGTTAGTATTTATCAACAAGAAAAGTGGGGCCCGCCGTGGAGATTCTTTAAAACAACGGTTAAACATGCTTTTGAATCCTGTGCAGGTTTTTGAGTTGAGTTCTACACAAGGACCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCGCACTTCAAGGTTCTTGTATGTGGGGGCGACGGAACTGTTGGTTGGGTCTTAAACTGTATAGACAAGCAAAATTTTGTTTCACCTCCCCCAGTTGCTATTCTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGTACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCAATAGTGGATCAACATGGAAAGCAACTCAAATCTCCACAATTTATGAATAACTATCTAGGAATTGGATGTGATGCTAAGGTTGCCCTTGACATACATAATTTACGGGAGGAAAATCCAGAAAAATTTTATAACCAGTTTATGAACAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGATAGGACATTCGCAGACATTCCATGGCAAGTTCGTGTGGAAGTGGATGGTGCTGAGGTAGAGGTTCCTGAGGATGCAGAAGGTGTGCTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTTTGGCATAACGAGGACGAGACTTTTGATAATTTTGATTCACAGTCCATGCATGATAAACTACTCGAGGTTGTCAGCATATCTGGGACTTGGCACCTCGGAAAGTTACAGGTGGGGCTTTCTCGAGCTAGACGACTTGCACAGGGGAAGTCGATTAGGATACAATTATGTGCTGCATTGCCTGTTCAAATTGATGGAGAGCCTTGGTTTCAGGAATTCCCGTGTACACTAGTCATATCCCACCATGGACAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTTGGTCACGCAGCTGCAATTATCACTGATGTTTTGGAGAGTGCTGAATCTAATAATGTAATTAATGCTTCACAAAAGCGAGTACTTCTCCAAGAAATGGCAAAGAGGTTAACTTGA

Protein sequence

MDEDWEILSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIARSKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNICGVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSSITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGTERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLLQEMAKRLT
Homology
BLAST of Lsi02G001070 vs. ExPASy Swiss-Prot
Match: Q39017 (Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 534/733 (72.85%), Postives = 613/733 (83.63%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTD----RLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMK 60
           MD+D E+    PSW SKNP D    R  + SCF AA++GILTIA+TAFQWRRNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHR 120
           AIARSK+NPK  H+VPVA H W L+ ++R KNL+CCVCLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C ICG AAH +CSS+A KDCKCVSM+GFEHV+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNG 300
           FLSSITHGANE+AS+  ASIR QSKK K + + S  +G SGS  D STESTAD+    NG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMP 360
            H    N       +  + + D + K    PS+K+  +  QK E H L   L+YE+ ++P
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQK-EYHALRSKLKYELADLP 360

Query: 361 SDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVL 420
           SD+RPLLVFINKKSGA+RGDSL+QRL++ LNPVQVFELSS QGPE GL+LFRKVPHF+VL
Sbjct: 361 SDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVL 420

Query: 421 VCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL 480
           VCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL
Sbjct: 421 VCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVL 480

Query: 481 HHVENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFY 540
            ++E+AAVTVLDRWKV+I++Q GKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY
Sbjct: 481 QNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFY 540

Query: 541 NQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVD 600
           +QFMNKVLYAREGA+SIMDRTF D PWQVRVEVDG ++EVPEDAEG+LVANIGSYMGGVD
Sbjct: 541 SQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVD 600

Query: 601 LWHNEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAAL 660
           LW NEDET++NFD QSMHDK++EVVSISGTWHLGKLQVGLSRARRLAQG +++IQLCA L
Sbjct: 601 LWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPL 660

Query: 661 PVQIDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQ 720
           PVQIDGEPW Q+ PCTL ISHHGQAFMLKRA EEPLGHAAAIITDVLE+AE+N VINASQ
Sbjct: 661 PVQIDGEPWNQQ-PCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQ 720

Query: 721 KRVLLQEMAKRLT 728
           KR LLQEMA RLT
Sbjct: 721 KRTLLQEMALRLT 728

BLAST of Lsi02G001070 vs. ExPASy Swiss-Prot
Match: Q9FFN7 (Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.8e-200
Identity = 355/719 (49.37%), Postives = 479/719 (66.62%), Query Frame = 0

Query: 12  WNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIARSKRNPKKTHRVPV 71
           W   +  D  F+        +G+L + +T  +W++  +L+W+KA AR K+   K  RVP+
Sbjct: 11  WLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKVWKRLRVPL 70

Query: 72  AAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNICGVAAHLSCSSNAQ 131
           + H W  +     +  +CCVCL  + P Q +    +    +HRC +CGVAAH  CSS+A 
Sbjct: 71  SHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAHFYCSSSAA 130

Query: 132 KDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQR 191
           KDCKCV+  G +HV H W+ RW  + D  D T+FC YC+EPC   F+  SP+W CLWCQR
Sbjct: 131 KDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQR 190

Query: 192 LVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSSITHGANEIASSVR 251
           L+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    NE+A S+R
Sbjct: 191 LIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---KNELA-SIR 250

Query: 252 ASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGTERNCNGSRTSEGP 311
             +R +  + K+    S+    +G + + S      +    NG     +     R+ +  
Sbjct: 251 GHVRRKRHRGKNGNGQSL----NGKLLEDSVSDPVKT--VVNGL--VVKKLRRDRSIDCL 310

Query: 312 HQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSRPLLVFINKKSGAR 371
            Q  D+ +       L+     ++++++  L    ++ ++++P D+RPLLVFIN KSG +
Sbjct: 311 KQVSDMPNAK----GLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARPLLVFINAKSGGQ 370

Query: 372 RGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDK 431
            G  L +RLNMLLNPVQVFEL S QGP++GL L  KV +F+VLVCGGDGTV WVL+ I+K
Sbjct: 371 LGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEK 430

Query: 432 QNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVA 491
           +NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L  +++AAVT+LDRW V 
Sbjct: 431 RNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVK 490

Query: 492 IVDQHGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA 551
           IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA
Sbjct: 491 IVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGA 550

Query: 552 KSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDS 611
           + IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ E  DNF  
Sbjct: 551 RDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSI 610

Query: 612 QSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFP 671
           Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQGK IRI + +  PVQIDGEP+ Q+ P
Sbjct: 611 QCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQ-P 670

Query: 672 CTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLLQEMAKRLT 728
             L I+HHGQ FML+RA +EP GHAAAI+ +VL  AE   VINASQK+VLLQ+MA  L+
Sbjct: 671 GCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 712

BLAST of Lsi02G001070 vs. ExPASy Swiss-Prot
Match: Q6NS52 (Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 5.9e-71
Identity = 147/352 (41.76%), Postives = 196/352 (55.68%), Query Frame = 0

Query: 357 SRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVC 416
           + PLLVF+N KSG ++G+ + ++   LLNP QV+ LS   GP  GL+ FR VP F+VL C
Sbjct: 434 THPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSG-NGPMPGLHFFRDVPDFRVLAC 493

Query: 417 GGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHH 476
           GGDGTVGW+L+CI+K N V  PPVAILP GTGNDLAR L WGGG         L  +L  
Sbjct: 494 GGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGY----EGENLMKILKD 553

Query: 477 VENAAVTVLDRWKVAIVDQHGKQLKSP---QFMNNYLGIGCDAKVALDIHNLREENPEKF 536
           +E++   +LDRWK  +      +   P     +NNY  IG DA +A   H +RE++PEKF
Sbjct: 554 IESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKF 613

Query: 537 YNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVE-VPEDAEGVLVANIGSYMGG 596
            ++  NK  Y   G       T   +   V +E DG +++ +    EG+ + NI S  GG
Sbjct: 614 NSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGG 673

Query: 597 VDLWHNE---------------------DETFDNFDSQSMHDKLLEVVSISGTWHLGKLQ 656
            +LW                        D     F SQ + D+LLEVV + G   +G++ 
Sbjct: 674 SNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIY 733

Query: 657 VGL-SRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFPCTLVISHHGQAFML 683
            GL S  RRLAQ  S+ I+   +LP+QIDGEPW Q  PCT+ I+H  QA ML
Sbjct: 734 TGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQT-PCTIKITHKNQAPML 779

BLAST of Lsi02G001070 vs. ExPASy Swiss-Prot
Match: P49621 (Diacylglycerol kinase beta OS=Rattus norvegicus OX=10116 GN=Dgkb PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.3e-70
Identity = 146/352 (41.48%), Postives = 196/352 (55.68%), Query Frame = 0

Query: 357 SRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVC 416
           + PLLVF+N KSG ++G+ + ++   LLNP QV+ LS   GP  GL+ FR VP F+VL C
Sbjct: 433 THPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSG-NGPMPGLHFFRDVPDFRVLAC 492

Query: 417 GGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHH 476
           GGDGTVGW+L+CI+K N V  PPVAILP GTGNDLAR L WGGG         L  +L  
Sbjct: 493 GGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGY----EGENLMKILKD 552

Query: 477 VENAAVTVLDRWKVAIVDQHGKQLKSP---QFMNNYLGIGCDAKVALDIHNLREENPEKF 536
           +E++   +LDRWK  +      +   P     +NNY  IG DA +A   H +RE++PEKF
Sbjct: 553 IESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKF 612

Query: 537 YNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVE-VPEDAEGVLVANIGSYMGG 596
            ++  NK  Y   G       T   +   V +E DG +++ +    +G+ + NI S  GG
Sbjct: 613 NSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLQGIAILNIPSMHGG 672

Query: 597 VDLWHNE---------------------DETFDNFDSQSMHDKLLEVVSISGTWHLGKLQ 656
            +LW                        D     F SQ + D+LLEVV + G   +G++ 
Sbjct: 673 SNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIY 732

Query: 657 VGL-SRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFPCTLVISHHGQAFML 683
            GL S  RRLAQ  S+ I+   +LP+QIDGEPW Q  PCT+ I+H  QA ML
Sbjct: 733 TGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQT-PCTIKITHKNQAPML 778

BLAST of Lsi02G001070 vs. ExPASy Swiss-Prot
Match: P49620 (Diacylglycerol kinase gamma OS=Rattus norvegicus OX=10116 GN=Dgkg PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.7e-70
Identity = 148/358 (41.34%), Postives = 205/358 (57.26%), Query Frame = 0

Query: 346 LRYEVIEMPSDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLF 405
           ++Y++I  P  + PLLV +N KSG R+G+ + Q+ + LLNP QVF L    GP  GL  F
Sbjct: 419 MQYKIIPSPG-THPLLVLVNPKSGGRQGERILQKFHYLLNPKQVFNLDK-GGPTPGLNFF 478

Query: 406 RKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVE 465
           +  P F+VL CGGDGTVGW+L+CIDK NF   PPVA+LP GTGNDLAR L WGGG     
Sbjct: 479 QDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPVAVLPLGTGNDLARCLRWGGGY---- 538

Query: 466 RQGGLCTVLHHVENAAVTVLDRWKVAIVD----QHGKQLKSPQFMNNYLGIGCDAKVALD 525
             G L  +L  +E + + +LDRW + ++     ++G Q+     MNNY  IG DA +A  
Sbjct: 539 EGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVENGDQVPY-NIMNNYFSIGVDASIAHR 598

Query: 526 IHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPE-DAEG 585
            H +RE++PEKF ++  NK+ Y   G       T   +   + +E DG EV++     EG
Sbjct: 599 FHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVEVDLSNIFLEG 658

Query: 586 VLVANIGSYMGGVDLW----HNEDETFDNFDS-----------QSMHDKLLEVVSISGTW 645
           + + NI S  GG +LW     N     ++  S           Q + D+LLEVV + G  
Sbjct: 659 IAILNIPSMYGGTNLWGETKKNRAVIRESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAM 718

Query: 646 HLGKLQVGL-SRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFPCTLVISHHGQAFML 683
            +G++  GL S  RRLAQ  S+ I+    LP+Q+DGEPW Q  PC + I+H  QA M+
Sbjct: 719 EMGQIYTGLKSAGRRLAQCSSVTIRTKKLLPMQVDGEPWMQP-PCMIKITHKNQAPMM 768

BLAST of Lsi02G001070 vs. ExPASy TrEMBL
Match: A0A5D3BKN2 (Diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001240 PE=3 SV=1)

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 696/730 (95.34%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEDWEI  L PSWNSKNPTDRLFVISCF AA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 300
           ITHGANEIASSVRASIR+QSKK+KHSRKPS+ H+G SG++RDMSTESTADSH   NGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 360
           TERNCNGSRTSEG HQNGDI DKSISN SLKK+S+LN KDETHILGMNLRYEVIEMPSDS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660
           NEDETFDNFDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAKRLT 728
           LLQEMAKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Lsi02G001070 vs. ExPASy TrEMBL
Match: A0A1S3BKC1 (Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1)

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 696/730 (95.34%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEDWEI  L PSWNSKNPTDRLFVISCF AA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 300
           ITHGANEIASSVRASIR+QSKK+KHSRKPS+ H+G SG++RDMSTESTADSH   NGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 360
           TERNCNGSRTSEG HQNGDI DKSISN SLKK+S+LN KDETHILGMNLRYEVIEMPSDS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660
           NEDETFDNFDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAKRLT 728
           LLQEMAKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Lsi02G001070 vs. ExPASy TrEMBL
Match: A0A0A0LSU0 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G039060 PE=3 SV=1)

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 697/730 (95.48%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 2   DEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIARS 61
           DE+WEI  L PSWNSKNPTDRLFVISCF AAIIGILTIAFTAFQWRRNINLSWM+AIARS
Sbjct: 3   DEEWEIGLLFPSWNSKNPTDRLFVISCFSAAIIGILTIAFTAFQWRRNINLSWMRAIARS 62

Query: 62  K-RNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 121
           K RNPKKT RVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 63  KRRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 122

Query: 122 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 181
           GVAAHLSCSSNAQKDCKCVSMIGF+HVMHQWAVRWTEITDQ DETSFCSYCEEPCSGSFL
Sbjct: 123 GVAAHLSCSSNAQKDCKCVSMIGFDHVMHQWAVRWTEITDQSDETSFCSYCEEPCSGSFL 182

Query: 182 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 241
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 183 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRI-SGGFLSS 242

Query: 242 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 301
           ITHGANEIASSVRASIR+QSKK+KHSRKPSI H+G SG++RDMSTESTADSHH  NGYHG
Sbjct: 243 ITHGANEIASSVRASIRSQSKKSKHSRKPSIHHTGSSGNLRDMSTESTADSHHRVNGYHG 302

Query: 302 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 361
           TERNCNGSRTSEG HQNGDI+DKSISN SLKKNS+LN KDETHILGMNLRYEVIEMPSD+
Sbjct: 303 TERNCNGSRTSEGRHQNGDINDKSISNTSLKKNSSLNHKDETHILGMNLRYEVIEMPSDA 362

Query: 362 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 421
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 363 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 422

Query: 422 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 481
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 423 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 482

Query: 482 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 541
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 483 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 542

Query: 542 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 601
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 543 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 602

Query: 602 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 661
           NEDETFDNFD+QSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 603 NEDETFDNFDAQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 662

Query: 662 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 721
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 663 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 722

Query: 722 LLQEMAKRLT 728
           LLQEMAKRLT
Sbjct: 723 LLQEMAKRLT 731

BLAST of Lsi02G001070 vs. ExPASy TrEMBL
Match: A0A1S4DTP2 (Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1)

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 693/727 (95.32%), Postives = 708/727 (97.39%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEDWEI  L PSWNSKNPTDRLFVISCF AA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 300
           ITHGANEIASSVRASIR+QSKK+KHSRKPS+ H+G SG++RDMSTESTADSH   NGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 360
           TERNCNGSRTSEG HQNGDI DKSISN SLKK+S+LN KDETHILGMNLRYEVIEMPSDS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660
           NEDETFDNFDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAK 725
           LLQEMAK
Sbjct: 721 LLQEMAK 726

BLAST of Lsi02G001070 vs. ExPASy TrEMBL
Match: A0A6J1E7Q5 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1)

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 686/729 (94.10%), Postives = 706/729 (96.84%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEI  L PSWNSKNPTDRLFVISC  AAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT++VPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGT 300
           ITHGANEIASSVRASIR+QSKKNKHSRKPSIH+  SGSIRDMSTESTADSHH  NGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSR 360
           ERN NGSRTSE  HQNGDIDDK+ISN S KKNS+ NQKD TH+ GMNLRYEVIE+P DSR
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQI 660
           EDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720
           DGEPWFQ+ PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAKRLT 728
           LQE+AKRLT
Sbjct: 721 LQEIAKRLT 728

BLAST of Lsi02G001070 vs. NCBI nr
Match: XP_038893796.1 (diacylglycerol kinase 1 [Benincasa hispida])

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 695/729 (95.34%), Postives = 709/729 (97.26%), Query Frame = 0

Query: 1   MDEDWE--ILSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDED E  +L PSWNSKNPTDRLFVISCF AAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDSEMGLLFPSWNSKNPTDRLFVISCFSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK VSPSQTLGPMVAS+SFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKSVSPSQTLGPMVASNSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFEHV+HQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVIHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSS GFLS+
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSSGFLST 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGT 300
           ITHGANEIASSVRASIRNQSKK+KHSRKPS+ +G SGS+RDMSTESTADSH   NGYHGT
Sbjct: 241 ITHGANEIASSVRASIRNQSKKSKHSRKPSVPTGSSGSLRDMSTESTADSHRTVNGYHGT 300

Query: 301 ERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSR 360
           ERNCNG+RTS+G HQN DIDDKSISNPS KKNS+LN KDETHILGMNLRYE+IEMPSDSR
Sbjct: 301 ERNCNGNRTSDGRHQNVDIDDKSISNPSFKKNSSLNHKDETHILGMNLRYELIEMPSDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFK+LVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKILVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480

Query: 481 NAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQHGKQLKS QFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQHGKQLKSSQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQI 660
           EDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQI
Sbjct: 601 EDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQI 660

Query: 661 DGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720
           DGEPWFQ+ PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL
Sbjct: 661 DGEPWFQDLPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAKRLT 728
           LQEMAKRLT
Sbjct: 721 LQEMAKRLT 729

BLAST of Lsi02G001070 vs. NCBI nr
Match: XP_008448453.1 (PREDICTED: diacylglycerol kinase 1 isoform X2 [Cucumis melo] >TYJ98925.1 diacylglycerol kinase 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 696/730 (95.34%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEDWEI  L PSWNSKNPTDRLFVISCF AA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 300
           ITHGANEIASSVRASIR+QSKK+KHSRKPS+ H+G SG++RDMSTESTADSH   NGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 360
           TERNCNGSRTSEG HQNGDI DKSISN SLKK+S+LN KDETHILGMNLRYEVIEMPSDS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660
           NEDETFDNFDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAKRLT 728
           LLQEMAKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Lsi02G001070 vs. NCBI nr
Match: XP_011650112.1 (diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744670.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744672.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744674.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 697/730 (95.48%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 2   DEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIARS 61
           DE+WEI  L PSWNSKNPTDRLFVISCF AAIIGILTIAFTAFQWRRNINLSWM+AIARS
Sbjct: 3   DEEWEIGLLFPSWNSKNPTDRLFVISCFSAAIIGILTIAFTAFQWRRNINLSWMRAIARS 62

Query: 62  K-RNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 121
           K RNPKKT RVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 63  KRRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 122

Query: 122 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 181
           GVAAHLSCSSNAQKDCKCVSMIGF+HVMHQWAVRWTEITDQ DETSFCSYCEEPCSGSFL
Sbjct: 123 GVAAHLSCSSNAQKDCKCVSMIGFDHVMHQWAVRWTEITDQSDETSFCSYCEEPCSGSFL 182

Query: 182 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 241
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 183 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRI-SGGFLSS 242

Query: 242 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 301
           ITHGANEIASSVRASIR+QSKK+KHSRKPSI H+G SG++RDMSTESTADSHH  NGYHG
Sbjct: 243 ITHGANEIASSVRASIRSQSKKSKHSRKPSIHHTGSSGNLRDMSTESTADSHHRVNGYHG 302

Query: 302 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 361
           TERNCNGSRTSEG HQNGDI+DKSISN SLKKNS+LN KDETHILGMNLRYEVIEMPSD+
Sbjct: 303 TERNCNGSRTSEGRHQNGDINDKSISNTSLKKNSSLNHKDETHILGMNLRYEVIEMPSDA 362

Query: 362 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 421
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 363 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 422

Query: 422 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 481
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 423 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 482

Query: 482 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 541
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 483 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 542

Query: 542 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 601
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 543 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 602

Query: 602 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 661
           NEDETFDNFD+QSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 603 NEDETFDNFDAQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 662

Query: 662 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 721
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 663 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 722

Query: 722 LLQEMAKRLT 728
           LLQEMAKRLT
Sbjct: 723 LLQEMAKRLT 731

BLAST of Lsi02G001070 vs. NCBI nr
Match: XP_016899064.1 (PREDICTED: diacylglycerol kinase 1 isoform X1 [Cucumis melo] >XP_016899065.1 PREDICTED: diacylglycerol kinase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 693/727 (95.32%), Postives = 708/727 (97.39%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEDWEI  L PSWNSKNPTDRLFVISCF AA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT RVPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSI-HSGPSGSIRDMSTESTADSHHASNGYHG 300
           ITHGANEIASSVRASIR+QSKK+KHSRKPS+ H+G SG++RDMSTESTADSH   NGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDS 360
           TERNCNGSRTSEG HQNGDI DKSISN SLKK+S+LN KDETHILGMNLRYEVIEMPSDS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660
           NEDETFDNFDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQE PCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAK 725
           LLQEMAK
Sbjct: 721 LLQEMAK 726

BLAST of Lsi02G001070 vs. NCBI nr
Match: XP_023519698.1 (diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 685/729 (93.96%), Postives = 707/729 (96.98%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEI  L PSWNSKNPTDRLFVISC  AAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT++VPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGT 300
           ITHGANEIASSVRASIR+QSKKNKHSR+PSIH+  SGSIRDMSTESTADSHH  NGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRRPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSR 360
           ERN NGSRTSE  HQNGDI+DK+ISNPS KKNS+ NQKDETH+ GMNLRYEVIE+P DSR
Sbjct: 301 ERNINGSRTSELQHQNGDINDKNISNPSFKKNSSFNQKDETHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAV VLDRWKVAIVDQ GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVAVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDG EVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQI 660
           EDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720
           DGEPWFQ+ PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAKRLT 728
           LQE+AKRLT
Sbjct: 721 LQEIAKRLT 728

BLAST of Lsi02G001070 vs. TAIR 10
Match: AT5G07920.1 (diacylglycerol kinase1 )

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 534/733 (72.85%), Postives = 613/733 (83.63%), Query Frame = 0

Query: 1   MDEDWEI--LSPSWNSKNPTD----RLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMK 60
           MD+D E+    PSW SKNP D    R  + SCF AA++GILTIA+TAFQWRRNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTHRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHR 120
           AIARSK+NPK  H+VPVA H W L+ ++R KNL+CCVCLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGVAAHLSCSSNAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C ICG AAH +CSS+A KDCKCVSM+GFEHV+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNG 300
           FLSSITHGANE+AS+  ASIR QSKK K + + S  +G SGS  D STESTAD+    NG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMP 360
            H    N       +  + + D + K    PS+K+  +  QK E H L   L+YE+ ++P
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQK-EYHALRSKLKYELADLP 360

Query: 361 SDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVL 420
           SD+RPLLVFINKKSGA+RGDSL+QRL++ LNPVQVFELSS QGPE GL+LFRKVPHF+VL
Sbjct: 361 SDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVL 420

Query: 421 VCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL 480
           VCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL
Sbjct: 421 VCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVL 480

Query: 481 HHVENAAVTVLDRWKVAIVDQHGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFY 540
            ++E+AAVTVLDRWKV+I++Q GKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY
Sbjct: 481 QNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFY 540

Query: 541 NQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVD 600
           +QFMNKVLYAREGA+SIMDRTF D PWQVRVEVDG ++EVPEDAEG+LVANIGSYMGGVD
Sbjct: 541 SQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVD 600

Query: 601 LWHNEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAAL 660
           LW NEDET++NFD QSMHDK++EVVSISGTWHLGKLQVGLSRARRLAQG +++IQLCA L
Sbjct: 601 LWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPL 660

Query: 661 PVQIDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQ 720
           PVQIDGEPW Q+ PCTL ISHHGQAFMLKRA EEPLGHAAAIITDVLE+AE+N VINASQ
Sbjct: 661 PVQIDGEPWNQQ-PCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQ 720

Query: 721 KRVLLQEMAKRLT 728
           KR LLQEMA RLT
Sbjct: 721 KRTLLQEMALRLT 728

BLAST of Lsi02G001070 vs. TAIR 10
Match: AT5G63770.1 (diacylglycerol kinase 2 )

HSP 1 Score: 700.7 bits (1807), Expect = 1.3e-201
Identity = 355/719 (49.37%), Postives = 479/719 (66.62%), Query Frame = 0

Query: 12  WNSKNPTDRLFVISCFCAAIIGILTIAFTAFQWRRNINLSWMKAIARSKRNPKKTHRVPV 71
           W   +  D  F+        +G+L + +T  +W++  +L+W+KA AR K+   K  RVP+
Sbjct: 11  WLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKVWKRLRVPL 70

Query: 72  AAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNICGVAAHLSCSSNAQ 131
           + H W  +     +  +CCVCL  + P Q +    +    +HRC +CGVAAH  CSS+A 
Sbjct: 71  SHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAHFYCSSSAA 130

Query: 132 KDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQR 191
           KDCKCV+  G +HV H W+ RW  + D  D T+FC YC+EPC   F+  SP+W CLWCQR
Sbjct: 131 KDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQR 190

Query: 192 LVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRTSSGGFLSSITHGANEIASSVR 251
           L+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    NE+A S+R
Sbjct: 191 LIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---KNELA-SIR 250

Query: 252 ASIRNQSKKNKHSRKPSIHSGPSGSIRDMSTESTADSHHASNGYHGTERNCNGSRTSEGP 311
             +R +  + K+    S+    +G + + S      +    NG     +     R+ +  
Sbjct: 251 GHVRRKRHRGKNGNGQSL----NGKLLEDSVSDPVKT--VVNGL--VVKKLRRDRSIDCL 310

Query: 312 HQNGDIDDKSISNPSLKKNSTLNQKDETHILGMNLRYEVIEMPSDSRPLLVFINKKSGAR 371
            Q  D+ +       L+     ++++++  L    ++ ++++P D+RPLLVFIN KSG +
Sbjct: 311 KQVSDMPNAK----GLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARPLLVFINAKSGGQ 370

Query: 372 RGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDK 431
            G  L +RLNMLLNPVQVFEL S QGP++GL L  KV +F+VLVCGGDGTV WVL+ I+K
Sbjct: 371 LGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEK 430

Query: 432 QNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVA 491
           +NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L  +++AAVT+LDRW V 
Sbjct: 431 RNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVK 490

Query: 492 IVDQHGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA 551
           IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA
Sbjct: 491 IVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGA 550

Query: 552 KSIMDRTFADIPWQVRVEVDGAEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDS 611
           + IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ E  DNF  
Sbjct: 551 RDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSI 610

Query: 612 QSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFP 671
           Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQGK IRI + +  PVQIDGEP+ Q+ P
Sbjct: 611 QCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQ-P 670

Query: 672 CTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLLQEMAKRLT 728
             L I+HHGQ FML+RA +EP GHAAAI+ +VL  AE   VINASQK+VLLQ+MA  L+
Sbjct: 671 GCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 712

BLAST of Lsi02G001070 vs. TAIR 10
Match: AT5G63770.2 (diacylglycerol kinase 2 )

HSP 1 Score: 581.6 bits (1498), Expect = 8.6e-166
Identity = 302/573 (52.71%), Postives = 398/573 (69.46%), Query Frame = 0

Query: 158 DQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRL 217
           D  D T+FC YC+EPC   F+  SP+W CLWCQRL+HV CH  M  E+GD CDLGS RR+
Sbjct: 3   DNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRV 62

Query: 218 ILSPLYVKESNRTSSGGFLSSITHGANEIASSVRASIRNQSKKNKHSRKPSIHSGPSGSI 277
           ILSP++VK +      G L++I    NE+A S+R  +R +  + K+    S+    +G +
Sbjct: 63  ILSPVHVKLNEANGVDGVLTTI---KNELA-SIRGHVRRKRHRGKNGNGQSL----NGKL 122

Query: 278 RDMSTESTADSHHASNGYHGTERNCNGSRTSEGPHQNGDIDDKSISNPSLKKNSTLNQKD 337
            + S      +    NG     +     R+ +   Q  D+ +       L+     ++++
Sbjct: 123 LEDSVSDPVKT--VVNGL--VVKKLRRDRSIDCLKQVSDMPNAK----GLQNGIGGHKRN 182

Query: 338 ETHILGMNLRYEVIEMPSDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQG 397
           ++  L    ++ ++++P D+RPLLVFIN KSG + G  L +RLNMLLNPVQVFEL S QG
Sbjct: 183 KSAALNFMKKFSLVDLPPDARPLLVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQG 242

Query: 398 PESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNW 457
           P++GL L  KV +F+VLVCGGDGTV WVL+ I+K+NF SPPPVAILP GTGNDL+RVL W
Sbjct: 243 PDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQW 302

Query: 458 GGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQL---KSPQFMNNYLGIGC 517
           G G+  V+ QG L T L  +++AAVT+LDRW V IV++  ++    +  +FM NYLGIGC
Sbjct: 303 GRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGC 362

Query: 518 DAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGAEVEV 577
           DAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR  AD+PWQV +EVDG ++E+
Sbjct: 363 DAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEI 422

Query: 578 PEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDSQSMHDKLLEVVSISGTWHLGKLQVGL 637
           P+D+EG++V NIGSYMGGVDLW N+ E  DNF  Q MHDK LEVV + G WHLGKLQVGL
Sbjct: 423 PKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGL 482

Query: 638 SRARRLAQGKSIRIQLCAALPVQIDGEPWFQEFPCTLVISHHGQAFMLKRAVEEPLGHAA 697
           S+ARRLAQGK IRI + +  PVQIDGEP+ Q+ P  L I+HHGQ FML+RA +EP GHAA
Sbjct: 483 SQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQ-PGCLEITHHGQVFMLRRASDEPRGHAA 542

Query: 698 AIITDVLESAESNNVINASQKRVLLQEMAKRLT 728
           AI+ +VL  AE   VINASQK+VLLQ+MA  L+
Sbjct: 543 AIMNEVLLDAECKGVINASQKKVLLQQMALHLS 558

BLAST of Lsi02G001070 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 161.8 bits (408), Expect = 2.1e-39
Identity = 112/377 (29.71%), Postives = 170/377 (45.09%), Query Frame = 0

Query: 359 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELS------------------STQGPES 418
           PL+VF+N KSG R+G  +K+RL  L++  QV++L+                  +++G E 
Sbjct: 90  PLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDEC 149

Query: 419 GLYLFRKVPHFKVLVCGGDGTVGWVLNCIDK---QNFVSPPPVAILPAGTGNDLARVLNW 478
              +  K+   +++V GGDGTVGWVL C+ +   QN +  PPV+I+P GTGNDL+R   W
Sbjct: 150 AKEIREKM---RIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGW 209

Query: 479 GGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKSPQFMN---------- 538
           GG       +  +   LH    A ++ LD W + I    G+ +  P  +           
Sbjct: 210 GGSF-PFAWKSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQN 269

Query: 539 -------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA 598
                              NY  IG DA+VA   H+LR E P        NK++Y+  G 
Sbjct: 270 LEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGC 329

Query: 599 KS-------IMD---RTFADIPWQVRVEVDGAE---VEVPEDAEGVLVANIGSYMGGVDL 658
                    I D   R   +I      ++D +E   V VP+    V+  N+ SY  G + 
Sbjct: 330 SQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNP 389

Query: 659 WHNEDETF---DNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCA 666
           W N  + +     F      D LLE+  +   WH   + V L  A+ +AQ  +IR+++  
Sbjct: 390 WGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRG 449

BLAST of Lsi02G001070 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 155.6 bits (392), Expect = 1.5e-37
Identity = 106/374 (28.34%), Postives = 156/374 (41.71%), Query Frame = 0

Query: 359 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPE---SGLYLFRKV------- 418
           P++VFIN  SG R G  LK+RL  L++  QVF+L+  +  E    GL    KV       
Sbjct: 91  PMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDEC 150

Query: 419 -----PHFKVLVCGGDGTVGWVLNC---IDKQNFVSPPPVAILPAGTGNDLARVLNWGGG 478
                   +++V GGDGTVGWVL C   ++K      PPV ++P GTGNDL+R   WGG 
Sbjct: 151 AKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGS 210

Query: 479 LGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKSPQFMN------------- 538
                R   +   LH      V  LD WK+ +    G+ +  P  +              
Sbjct: 211 FPFAWR-SAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSLKPAEENELDQGLDA 270

Query: 539 ----------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKS- 598
                           NYL IG DA+VA   H+LR   P        NK++Y+  G    
Sbjct: 271 GIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQG 330

Query: 599 ------IMDRTFADIPWQVRVEV------DGAEVEVPEDAEGVLVANIGSYMGGVDLWHN 658
                 + D     +   +++ +         E+ VP++   ++  N+ SY  G   W N
Sbjct: 331 WFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGN 390

Query: 659 EDETF---DNFDSQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAA-- 666
               +     F      D L+E+      WH   +   L  A+ +AQ  ++R +L     
Sbjct: 391 LKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDW 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q390170.0e+0072.85Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2[more]
Q9FFN71.8e-20049.37Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1[more]
Q6NS525.9e-7141.76Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2[more]
P496211.3e-7041.48Diacylglycerol kinase beta OS=Rattus norvegicus OX=10116 GN=Dgkb PE=1 SV=1[more]
P496201.7e-7041.34Diacylglycerol kinase gamma OS=Rattus norvegicus OX=10116 GN=Dgkg PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BKN20.0e+0095.34Diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold24... [more]
A0A1S3BKC10.0e+0095.34Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1[more]
A0A0A0LSU00.0e+0095.48Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G039060 PE=3 SV=1[more]
A0A1S4DTP20.0e+0095.32Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1[more]
A0A6J1E7Q50.0e+0094.10Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038893796.10.0e+0095.34diacylglycerol kinase 1 [Benincasa hispida][more]
XP_008448453.10.0e+0095.34PREDICTED: diacylglycerol kinase 1 isoform X2 [Cucumis melo] >TYJ98925.1 diacylg... [more]
XP_011650112.10.0e+0095.48diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744670.1 diacylglyce... [more]
XP_016899064.10.0e+0095.32PREDICTED: diacylglycerol kinase 1 isoform X1 [Cucumis melo] >XP_016899065.1 PRE... [more]
XP_023519698.10.0e+0093.96diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G07920.10.0e+0072.85diacylglycerol kinase1 [more]
AT5G63770.11.3e-20149.37diacylglycerol kinase 2 [more]
AT5G63770.28.6e-16652.71diacylglycerol kinase 2 [more]
AT5G57690.12.1e-3929.71diacylglycerol kinase 4 [more]
AT2G18730.11.5e-3728.34diacylglycerol kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 505..662
e-value: 5.7E-79
score: 278.3
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 505..662
e-value: 6.0E-55
score: 185.9
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainSMARTSM00109c1_12coord: 74..134
e-value: 2.7E-4
score: 30.3
coord: 147..209
e-value: 1.7E-6
score: 37.6
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPFAMPF00130C1_1coord: 147..207
e-value: 5.9E-11
score: 42.1
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 73..134
score: 8.698601
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 146..209
score: 7.9129
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 359..488
e-value: 4.5E-54
score: 195.6
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 359..459
e-value: 4.4E-28
score: 97.4
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 355..495
score: 40.943886
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 355..475
e-value: 2.5E-22
score: 80.8
NoneNo IPR availableGENE3D3.30.60.20coord: 145..204
e-value: 1.7E-9
score: 39.4
NoneNo IPR availableGENE3D2.60.200.40coord: 503..673
e-value: 2.4E-20
score: 75.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..335
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..335
NoneNo IPR availablePANTHERPTHR11255:SF91DIACYLGLYCEROL KINASEcoord: 15..726
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 15..726
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 358..679

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G001070.1Lsi02G001070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0016310 phosphorylation
biological_process GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0016301 kinase activity