Lsi02G000210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G000210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDNA polymerase epsilon subunit
Locationchr02: 271476 .. 274059 (-)
RNA-Seq ExpressionLsi02G000210
SyntenyLsi02G000210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGTGTCCTGCCCTCATCCAAAGGATATTTACTTCTCAAAGTTGGATTTGCATGTGGGATTTCATTCTTGTGGTTTACTTTCTTATTTGCTACCAAGCACAAGGATACCTTCCTTCTCAAGCGTTTTGGAAAATGTCCCGAACTTGTTTCATAGAAATATTTCAAATACTTTTCTTCATAGTAATAACGTGCTTTTCCTGGGCCTCTATTTTGTTAAGGTTGTTACATGTGGATTTCCTCCACTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGCTCTACCTAAAGAGGAGAATGTATCCTCTTTCTTTTTGTCTATAAAAAGGATTTCTAAATAAGCATTGGAAAGGAGGGCTATAGCTGACATTTTATTATGCTGAATTTTGGCTATTAGAAGGGTGTATACAACAACTTGTTTCCCTTAATTATCAATAGCTCAGGCTTGCAGATCTGGAGAAGAAAGCTGTTAATGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGTGACACATAACCTCTTTATATTTCACGTTTATTGTGTGCCTGTGAAGCTTGGTGGCCTTATCAAAATTTGTTCATGTGATAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTTGAAATGGTTCCTTCCTTATTTGTTTTGATGGGAAATTTTTGTTCCCGTCCATGTAATCTTGCCTTTAATTCTTTCTCAAGTCTCAGGTAAGTATTATCACAATTGTTTTCTGCGTATTGACATTGCTGTTATTGACATGAGTTCAGAGCAATAATGAGTTAGAATGTGCAGACTACAGTTTGGTAAGTTGGGAAAAATGATAGCAGCTCATCCACGATTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCCGATGATGCAGGTTTGTGTCGTTATTTGTTAAGAAGCTCCTAGCCTTGAGTTTAACTGTGCATGAGATTTTCTAAATTTTATTTACATGGAGTTTATAGGACCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACTGAAGAGCTCCAGATGCACGTCCCAAATGCCATTTTCTCAAGTAACCCTTGCAGGTGGATACTTTGATTGTTGACTTATAGCATTAATGGTCTTTCTAGTCTTAACTGTAGTATTATCCTGCCTGAGGGGCTTCACTTCTACAGCTTACTCGAGAAACAAATCATATTTTTTCCATTTGGAATAAGCAAACAGAACTGTATTTAATAGATTTAAAGGCTAGTGTTAATGGTCTGGATTTAGTTATTCAGTTTTGTTAACTTTATTGAATTTAAGAAATAAAAAATAATAATAAAGTTCATTTATTAACAAGTGAGAACAAAGCCTATGATGGACAAAATAAATGGTTCTTCAAAAGAGCTTCCGATTTTTTACTTAATTAAAAGAAATAAACCGATTTTAAGTGGGAATTATGCTCTCTTTGGTGAATGGTATGTTACTCCATGGTGGTGGATAATTGTCATATTTGAATGCAGGGTCAGATTCTACACCCAGGAAATTGTATTTTTCCGACAAGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCGACAGAAGAAACGAGTGATCCTTTTGAGCATGTGATATATTTTTTTTTCCTTTTATATATCTGTTATTTTTTCTAGTTATCAATTTATTGATTCTAGCCCTTTTGGATATTTATATTTGGAGGCCCTAAGGTTTCATGATTCTCTTGCAGCTTGTTGCGACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTACGATCACTGTCTTCATCTATATCCTACTCCTCATGTGGTACTTACCCATATCTCAATCTAGTGTTTTAATCAATCTATTTATACATGCAAAGTTGGATATCTTCTCTTTCGGCACCTGAAGACAGATAATGAACGAGGCTTCTAATGGATGATAATGTTCTGCTGCGATTGAACCTCATGGCTACATGAACTTACAGATTATTATATCAATCTTTATTTGGTTTGTGCAGATAGTTTTGGGGGATAGAAGCAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAAATTATTACAAAGTTTAGGCCAGATTCAATGCGGGCACTCCTTATGCGGCATAACAATTCATGTCGAATTTTGAAAGAGAAATCCTATAGTATCATTCTTTATCTTGGGAGTTGCATTGTGCCCACCAAGAATTACCACTGGCTAGAATTTCATTCTATGCTGAAGAAGAGTATTCGAAACTTGGTGTAGAATCAGGAATCTGTTTAGATGTACAGTTGTAAATCTGAGTAAAATATTCTTTAGTTTGTATACTTTTAACGGTGGATGGTGTATTGATAAACAACTAACCTAAAAATTCTCCCACATGAGACTCGTTCAGGGTTGTATGTTATAATGTTACAAGAAGATCATGGATA

mRNA sequence

ATGCAAGTGTCCTGCCCTCATCCAAAGGATATTTACTTCTCAAAGTTGGATTTGCATGTGGGATTTCATTCTTGTGGTTTACTTTCTTATTTGCTACCAAGCACAAGGATACCTTCCTTCTCAAGCGTTTTGGAAAATGTCCCGAACTTGTTTCATAGAAATATTTCAAATACTTTTCTTCATAGTAATAACGTGCTTTTCCTGGGCCTCTATTTTGTTAAGGTTGTTACATGTGGATTTCCTCCACTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGCTCTACCTAAAGAGGAGAATCTCAGGCTTGCAGATCTGGAGAAGAAAGCTGTTAATGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTTGAAATGGTTCCTTCCTTATTTGTTTTGATGGGAAATTTTTGTTCCCGTCCATGTAATCTTGCCTTTAATTCTTTCTCAAGTCTCAGACTACAGTTTGGTAAGTTGGGAAAAATGATAGCAGCTCATCCACGATTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCCGATGATGCAGGACCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACTGAAGAGCTCCAGATGCACGTCCCAAATGCCATTTTCTCAAGTAACCCTTGCAGGGTCAGATTCTACACCCAGGAAATTGTATTTTTCCGACAAGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCGACAGAAGAAACGAGTGATCCTTTTGAGCATCTTGTTGCGACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTACGATCACTGTCTTCATCTATATCCTACTCCTCATGTGATAGTTTTGGGGGATAGAAGCAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAAATTATTACAAAGTTTAGGCCAGATTCAATGCGGGCACTCCTTATGCGGCATAACAATTCATGTCGAATTTTGAAAGAGAAATCCTATAGTATCATTCTTTATCTTGGGAGTTGCATTGTGCCCACCAAGAATTACCACTGGCTAGAATTTCATTCTATGCTGAAGAAGAGTATTCGAAACTTGGTGTAGAATCAGGAATCTGTTTAGATGTACAGTTGTAAATCTGAGTAAAATATTCTTTAGTTTGTATACTTTTAACGGTGGATGGTGTATTGATAAACAACTAACCTAAAAATTCTCCCACATGAGACTCGTTCAGGGTTGTATGTTATAATGTTACAAGAAGATCATGGATA

Coding sequence (CDS)

ATGCAAGTGTCCTGCCCTCATCCAAAGGATATTTACTTCTCAAAGTTGGATTTGCATGTGGGATTTCATTCTTGTGGTTTACTTTCTTATTTGCTACCAAGCACAAGGATACCTTCCTTCTCAAGCGTTTTGGAAAATGTCCCGAACTTGTTTCATAGAAATATTTCAAATACTTTTCTTCATAGTAATAACGTGCTTTTCCTGGGCCTCTATTTTGTTAAGGTTGTTACATGTGGATTTCCTCCACTAGAGGAAAGGGACAAGTCTCTTAAATTGCTAGCAGGCCAGGACTTCTTTGGAGGTGGTGCTCTACCTAAAGAGGAGAATCTCAGGCTTGCAGATCTGGAGAAGAAAGCTGTTAATGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTTGAAATGGTTCCTTCCTTATTTGTTTTGATGGGAAATTTTTGTTCCCGTCCATGTAATCTTGCCTTTAATTCTTTCTCAAGTCTCAGACTACAGTTTGGTAAGTTGGGAAAAATGATAGCAGCTCATCCACGATTAAAAGAGCATAGCAAGTTTCTTTTTATTCCTGGTCCCGATGATGCAGGACCATCAACAGTTCTACCTAGGTGTGCTTTGCCGAAGTATTTAACTGAAGAGCTCCAGATGCACGTCCCAAATGCCATTTTCTCAAGTAACCCTTGCAGGGTCAGATTCTACACCCAGGAAATTGTATTTTTCCGACAAGACCTGCTTTATAGAATGCGCCGTTCCTGTCTCATACCACCTTCGACAGAAGAAACGAGTGATCCTTTTGAGCATCTTGTTGCGACCATAACTCATCAAAGTCATCTCTGCCCTCTTCCTCTGGTTATTCAACCAATTATCTGGAATTACGATCACTGTCTTCATCTATATCCTACTCCTCATGTGATAGTTTTGGGGGATAGAAGCAAGCAACAAGCATTCAAATACACAGGAATCACTTGCTTTAATCCTGGTTCCTTCACAAATGATAGCACTTTTGTGGCATATCGTCCTTGCAATCAAGAAGTTGAACTGTCTGCCTTGTAA

Protein sequence

MQVSCPHPKDIYFSKLDLHVGFHSCGLLSYLLPSTRIPSFSSVLENVPNLFHRNISNTFLHSNNVLFLGLYFVKVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Homology
[Close]
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match: Q500V9 (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 8.8e-140
Identity = 230/293 (78.50%), Postives = 261/293 (89.08%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           +V+TCGFPPLE+RDK+LK  +  DFFGGG L KEE ++LADLE++AVND FVILSDIWLD
Sbjct: 234 QVITCGFPPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLD 293

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
            EE M KLET+LDGFE+VE VPSLFV MGNFCSRPCNL+F S+SSLR QFGKLG+MI  H
Sbjct: 294 DEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNH 353

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKE+S+FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNAIFSSNPCRV+FY QEI
Sbjct: 354 PRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEI 413

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L 
Sbjct: 414 VFFRQDLLYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALR 473

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPH IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 474 LYPTPHTIVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

[Close]
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match: Q5ZKQ6 (DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 5.1e-55
Identity = 112/290 (38.62%), Postives = 165/290 (56.90%), Query Frame = 0 

Query: 79  GFPPLEERDKSLKLLAGQDFFGG-GALPKEENLRLADLEKKAVNDMFVILSDIWLDSEEA 138
           GFPP E    +       +FFGG  +   + + +L  LE +  + MFV LSD+WLD  E 
Sbjct: 241 GFPPTEPSATTRAFYGNINFFGGPSSASVKASAKLKQLEDENEDAMFVFLSDVWLDQAEV 300

Query: 139 MGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLK 198
           + KL T+  G+ +    P+ F   GNF S P     N   SL+     L  +I  +P + 
Sbjct: 301 LEKLHTMFSGYSSAP--PTCFFFCGNFSSAP--YGKNQIQSLKGSLKALADIICEYPSIH 360

Query: 199 EHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 258
           + S+F+F+PGP+D GP +VLPR  L + +T+E +  VP ++F++NPCRV++ TQEI+ FR
Sbjct: 361 KSSRFVFVPGPEDPGPGSVLPRPPLAENITQEFRQLVPFSVFTTNPCRVQYCTQEIIIFR 420

Query: 259 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 318
           +DL+ +M R+C+  PS+  + D   H V TI  Q HL PLPL + P+ W YD+ L +YP 
Sbjct: 421 EDLVNKMCRNCVRFPSS--SMDIPNHFVKTILSQGHLTPLPLYVSPVYWAYDYALRVYPV 480

Query: 319 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 367
           P ++V+ D+        T   C NPGSF     +F  + P N+ VE S L
Sbjct: 481 PDMLVIADKYDPFTVTNTDCLCINPGSFPRSGFSFKVFYPSNKTVEDSKL 524

[Close]
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match: A7YWS7 (DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.0e-52
Identity = 105/290 (36.21%), Postives = 161/290 (55.52%), Query Frame = 0 

Query: 79  GFPPLEERDKSLKLLAGQDFFGGGA-LPKEENLRLADLEKKAVNDMFVILSDIWLDSEEA 138
           GFPP E    +       +FFGG +    + + +L  LE +  + MFV +SD+WLD  E 
Sbjct: 241 GFPPTEPSSTTRAYYGNTNFFGGPSNTSVKTSAKLKQLEDENKDAMFVFISDVWLDQVEV 300

Query: 139 MGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLK 198
           + KL  +  G+      P+ F+L GNF S P     N   +L+     L  +I  +P + 
Sbjct: 301 LEKLHIMFSGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTLADIICGYPNIH 360

Query: 199 EHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 258
           + S+F+F+PGP+D G  ++LPR    + +T E +  VP ++F++NPCR+++ TQEI+ FR
Sbjct: 361 QSSRFVFVPGPEDPGFGSILPRPPFAESITNEFRQRVPFSVFTTNPCRIQYCTQEIIVFR 420

Query: 259 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 318
           +DL+ +M R+C+  PS+    D   H V T+  Q HL PLPL + P+ W YD+ L +YP 
Sbjct: 421 EDLVNKMCRNCVRFPSS--NLDIPNHFVKTVLSQGHLTPLPLYVCPVYWAYDYALRVYPV 480

Query: 319 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 367
           P ++V+ D+        T   C NPGSF     +F  + P N+ VE S L
Sbjct: 481 PDLLVIADKYDPFTLTNTECLCINPGSFPRSGFSFKVFYPSNKVVEDSKL 524

[Close]
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match: P56282 (DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 3.4e-51
Identity = 107/290 (36.90%), Postives = 161/290 (55.52%), Query Frame = 0 

Query: 79  GFPPLEERDKSLKLLAGQDFFGGGA-LPKEENLRLADLEKKAVNDMFVILSDIWLDSEEA 138
           GFPP E    +       +FFGG +    + + +L  LE++  + MFV LSD+WLD  E 
Sbjct: 241 GFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEV 300

Query: 139 MGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLK 198
           + KL  +  G+      P+ F+L GNF S P     N   +L+     L  +I  +P + 
Sbjct: 301 LEKLRIMFAGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKTLADIICEYPDIH 360

Query: 199 EHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 258
           + S+F+F+PGP+D G  ++LPR  L + +T E +  VP ++F++NPCR+++ TQEI  FR
Sbjct: 361 QSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFR 420

Query: 259 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 318
           +DL+ +M R+C+  PS+        H V TI  Q HL PLPL + P+ W YD+ L +YP 
Sbjct: 421 EDLVNKMCRNCVRFPSSNLAIP--NHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPV 480

Query: 319 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 367
           P ++V+ D+        T   C NPGSF     +F  + P N+ VE S L
Sbjct: 481 PDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKL 524

[Close]
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match: O54956 (DNA polymerase epsilon subunit 2 OS=Mus musculus OX=10090 GN=Pole2 PE=2 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 5.9e-51
Identity = 105/290 (36.21%), Postives = 162/290 (55.86%), Query Frame = 0 

Query: 79  GFPPLEERDKSLKLLAGQDFFGGGALPKEEN-LRLADLEKKAVNDMFVILSDIWLDSEEA 138
           GFPP E    +       +FFGG +    +   +L  LE +  + MFVI+SD+WLD  + 
Sbjct: 241 GFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQV 300

Query: 139 MGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLK 198
           + K   +  G+      P+ F+L GNF S P     N   +L+     L  +I  +P + 
Sbjct: 301 LEKFHVMFSGYSPAP--PTCFILCGNFSSAP--YGKNQVQALKDSLKSLADIICEYPSIH 360

Query: 199 EHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFR 258
           + S+F+F+PGP+D G  ++LPR  L + +T+E +  VP ++F++NPCR+++ TQEI+ FR
Sbjct: 361 QSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFR 420

Query: 259 QDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPT 318
           +DL+ +M R+C+  PS+  + D   H V TI  Q HL PLPL + P+ W YD+ L +YP 
Sbjct: 421 EDLVNKMCRNCVRFPSS--SLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPV 480

Query: 319 PHVIVLGDRSKQQAFKYTGITCFNPGSFTNDS-TFVAYRPCNQEVELSAL 367
           P ++V+ D+        T   C NPGSF      F  + P ++ VE S L
Sbjct: 481 PDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKL 524

[Close]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match: A0A6J1GCV3 (DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.0e-167
Identity = 284/293 (96.93%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+AVNDMFVILSDIWLD
Sbjct: 236 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLD 295

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA 
Sbjct: 296 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAAR 355

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 356 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 415

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 416 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 475

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 476 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

[Close]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match: A0A6J1KGF0 (DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.0e-167
Identity = 282/293 (96.25%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+A+NDMFVILSDIWLD
Sbjct: 236 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAINDMFVILSDIWLD 295

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIA+ 
Sbjct: 296 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIASR 355

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 356 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 415

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 416 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 475

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 476 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

[Close]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match: A0A5A7T342 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00330 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 3.0e-167
Identity = 283/293 (96.59%), Postives = 288/293 (98.29%), Query Frame = 0 

Query: 74   KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
            +VVTCGFPPLEERDKSLKLLAGQDFFGGG LPKEE LRLADLEKKAVNDMFVILSDIWLD
Sbjct: 858  QVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLD 917

Query: 134  SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
            SEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNSFSSLRLQFGKLGKMIAAH
Sbjct: 918  SEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAH 977

Query: 194  PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
            PRL +HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFYTQEI
Sbjct: 978  PRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEI 1037

Query: 254  VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
            VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 1038 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 1097

Query: 314  LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
            LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1098 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1150

[Close]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match: A0A5D3BIF9 (Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00380 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.7e-167
Identity = 288/333 (86.49%), Postives = 300/333 (90.09%), Query Frame = 0 

Query: 39   SFSSVLENVPNLFHRNISNTFLHSNNVL-----FLGLYFVKVVTCGFPPLEERDKSLKLL 98
            + S+ +    N +   I+      N ++      L     +VVTCGFPPLEERDKSLKLL
Sbjct: 819  NLSNAISFTGNFYDHKITTGLFTENTIIVAEGEMLVEGIFQVVTCGFPPLEERDKSLKLL 878

Query: 99   AGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEM 158
            AGQDFFGGG LPKEE LRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVE+
Sbjct: 879  AGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEV 938

Query: 159  VPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAHPRLKEHSKFLFIPGPDDAGP 218
            VPSLFVLMGNFCS PCN+AFNSFSSLRLQFGKLGKMIAAHPRL +HSKFLFIPGPDDAGP
Sbjct: 939  VPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAHPRLNKHSKFLFIPGPDDAGP 998

Query: 219  STVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPS 278
            STVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPS
Sbjct: 999  STVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEIVFFRQDLLYRMRRSCLIPPS 1058

Query: 279  TEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFK 338
            TEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFK
Sbjct: 1059 TEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLHLYPTPHVIVLGDRSKQQAFK 1118

Query: 339  YTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
            YTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1119 YTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1151

[Close]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match: A0A1S4DTQ5 (DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 282/293 (96.25%), Postives = 287/293 (97.95%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           +VVTCGFPPLEERDKSLKLLAGQDFFGGG LPKEE LRLADLEKKAVNDMFVILSDIWLD
Sbjct: 237 QVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLD 296

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNSFSSLRLQFGKLGKMIAAH
Sbjct: 297 SEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAH 356

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRL +HSKF FIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFYTQEI
Sbjct: 357 PRLNKHSKFXFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEI 416

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 417 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 476

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 477 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 529

[Close]
BLAST of Lsi02G000210 vs. NCBI nr
Match: KAG6607399.1 (DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 284/293 (96.93%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+AVNDMFVILSDIWLD
Sbjct: 213 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLD 272

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA 
Sbjct: 273 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAAR 332

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 333 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 392

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 393 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 452

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 453 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 505

[Close]
BLAST of Lsi02G000210 vs. NCBI nr
Match: XP_023525784.1 (DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 284/293 (96.93%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+AVNDMFVILSDIWLD
Sbjct: 236 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLD 295

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA 
Sbjct: 296 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAAR 355

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 356 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 415

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 416 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 475

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 476 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

[Close]
BLAST of Lsi02G000210 vs. NCBI nr
Match: XP_022949454.1 (DNA polymerase epsilon subunit B [Cucurbita moschata])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 284/293 (96.93%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+AVNDMFVILSDIWLD
Sbjct: 236 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLD 295

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA 
Sbjct: 296 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAAR 355

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 356 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 415

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 416 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 475

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 476 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 528

[Close]
BLAST of Lsi02G000210 vs. NCBI nr
Match: KAG7037068.1 (DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 284/293 (96.93%), Postives = 290/293 (98.98%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           KV+TCGFPPLE+RDKSLKLLAGQDFFGGG+L KEE LRLADLEK+AVNDMFVILSDIWLD
Sbjct: 229 KVITCGFPPLEDRDKSLKLLAGQDFFGGGSLTKEETLRLADLEKRAVNDMFVILSDIWLD 288

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
           SEEAMGKLETILDGFENVE+VPSLFVLMGNFCSRPCNLAFNS+SSLRLQFGKLGKMIAA 
Sbjct: 289 SEEAMGKLETILDGFENVEIVPSLFVLMGNFCSRPCNLAFNSYSSLRLQFGKLGKMIAAR 348

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI
Sbjct: 349 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 408

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 409 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 468

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 469 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 521

[Close]
BLAST of Lsi02G000210 vs. NCBI nr
Match: KAA0036041.1 (DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 597.8 bits (1540), Expect = 6.2e-167
Identity = 283/293 (96.59%), Postives = 288/293 (98.29%), Query Frame = 0 

Query: 74   KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
            +VVTCGFPPLEERDKSLKLLAGQDFFGGG LPKEE LRLADLEKKAVNDMFVILSDIWLD
Sbjct: 858  QVVTCGFPPLEERDKSLKLLAGQDFFGGGVLPKEETLRLADLEKKAVNDMFVILSDIWLD 917

Query: 134  SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
            SEEAMGKLETILDGFENVE+VPSLFVLMGNFCS PCN+AFNSFSSLRLQFGKLGKMIAAH
Sbjct: 918  SEEAMGKLETILDGFENVEVVPSLFVLMGNFCSHPCNIAFNSFSSLRLQFGKLGKMIAAH 977

Query: 194  PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
            PRL +HSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQ+HVPNA FSSNPCRVRFYTQEI
Sbjct: 978  PRLNKHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQVHVPNATFSSNPCRVRFYTQEI 1037

Query: 254  VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
            VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH
Sbjct: 1038 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 1097

Query: 314  LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
            LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL
Sbjct: 1098 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 1150

[Close]
BLAST of Lsi02G000210 vs. TAIR 10
Match: AT5G22110.1 (DNA polymerase epsilon subunit B2 )

HSP 1 Score: 498.0 bits (1281), Expect = 6.3e-141
Identity = 230/293 (78.50%), Postives = 261/293 (89.08%), Query Frame = 0 

Query: 74  KVVTCGFPPLEERDKSLKLLAGQDFFGGGALPKEENLRLADLEKKAVNDMFVILSDIWLD 133
           +V+TCGFPPLE+RDK+LK  +  DFFGGG L KEE ++LADLE++AVND FVILSDIWLD
Sbjct: 234 QVITCGFPPLEDRDKTLKAHSEYDFFGGGTLTKEEMIKLADLERQAVNDTFVILSDIWLD 293

Query: 134 SEEAMGKLETILDGFENVEMVPSLFVLMGNFCSRPCNLAFNSFSSLRLQFGKLGKMIAAH 193
            EE M KLET+LDGFE+VE VPSLFV MGNFCSRPCNL+F S+SSLR QFGKLG+MI  H
Sbjct: 294 DEEVMRKLETVLDGFESVETVPSLFVFMGNFCSRPCNLSFGSYSSLREQFGKLGRMIGNH 353

Query: 194 PRLKEHSKFLFIPGPDDAGPSTVLPRCALPKYLTEELQMHVPNAIFSSNPCRVRFYTQEI 253
           PRLKE+S+FLFIPGP+DAGPSTVLPRCALPKYLTEEL+  +PNAIFSSNPCRV+FY QEI
Sbjct: 354 PRLKENSRFLFIPGPEDAGPSTVLPRCALPKYLTEELRNIIPNAIFSSNPCRVKFYNQEI 413

Query: 254 VFFRQDLLYRMRRSCLIPPSTEETSDPFEHLVATITHQSHLCPLPLVIQPIIWNYDHCLH 313
           VFFRQDLLYRMRRSCL+ PS+EET+DPF+HLV TITHQSHLCPLPL++QPIIWNYDH L 
Sbjct: 414 VFFRQDLLYRMRRSCLVTPSSEETNDPFKHLVYTITHQSHLCPLPLMVQPIIWNYDHALR 473

Query: 314 LYPTPHVIVLGDRSKQQAFKYTGITCFNPGSFTNDSTFVAYRPCNQEVELSAL 367
           LYPTPH IVLGD+S+Q+  K+ G TCFNPGSF+ DSTFVAYRP  QEVELSAL
Sbjct: 474 LYPTPHTIVLGDKSEQEVCKFGGTTCFNPGSFSTDSTFVAYRPSTQEVELSAL 526

The following BLAST results are available for this feature:
BLAST of Lsi02G000210 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q500V98.8e-14078.50DNA polymerase epsilon subunit B OS=Arabidopsis thaliana OX=3702 GN=DPB2 PE=1 SV... [more]
Q5ZKQ65.1e-5538.62DNA polymerase epsilon subunit 2 OS=Gallus gallus OX=9031 GN=POLE2 PE=2 SV=1[more]
A7YWS79.0e-5236.21DNA polymerase epsilon subunit 2 OS=Bos taurus OX=9913 GN=POLE2 PE=2 SV=1[more]
P562823.4e-5136.90DNA polymerase epsilon subunit 2 OS=Homo sapiens OX=9606 GN=POLE2 PE=1 SV=2[more]
O549565.9e-5136.21DNA polymerase epsilon subunit 2 OS=Mus musculus OX=10090 GN=Pole2 PE=2 SV=2[more]
BLAST of Lsi02G000210 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GCV31.0e-16796.93DNA polymerase epsilon subunit OS=Cucurbita moschata OX=3662 GN=LOC111452793 PE=... [more]
A0A6J1KGF03.0e-16796.25DNA polymerase epsilon subunit OS=Cucurbita maxima OX=3661 GN=LOC111492993 PE=3 ... [more]
A0A5A7T3423.0e-16796.59Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3BIF96.7e-16786.49Glutamate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S4DTQ51.1e-16696.25DNA polymerase epsilon subunit OS=Cucumis melo OX=3656 GN=LOC103501415 PE=3 SV=1[more]
BLAST of Lsi02G000210 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6607399.12.1e-16796.93DNA polymerase epsilon subunit B, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023525784.12.1e-16796.93DNA polymerase epsilon subunit B [Cucurbita pepo subsp. pepo][more]
XP_022949454.12.1e-16796.93DNA polymerase epsilon subunit B [Cucurbita moschata][more]
KAG7037068.12.1e-16796.93DNA polymerase epsilon subunit B [Cucurbita argyrosperma subsp. argyrosperma][more]
KAA0036041.16.2e-16796.59DNA polymerase epsilon subunit 2 [Cucumis melo var. makuwa][more]
BLAST of Lsi02G000210 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G22110.16.3e-14178.50DNA polymerase epsilon subunit B2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007185DNA polymerase alpha/delta/epsilon, subunit BPFAMPF04042DNA_pol_E_Bcoord: 124..331
e-value: 8.5E-48
score: 162.5
NoneNo IPR availableGENE3D3.60.21.60coord: 136..362
e-value: 2.5E-6
score: 28.5
IPR016266DNA polymerase epsilon, subunit BPANTHERPTHR12708DNA POLYMERASE EPSILON SUBUNIT Bcoord: 65..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G000210.1Lsi02G000210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006261 DNA-dependent DNA replication
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0006536 glutamate metabolic process
biological_process GO:0051781 positive regulation of cell division
biological_process GO:0006260 DNA replication
cellular_component GO:0008622 epsilon DNA polymerase complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0070182 DNA polymerase binding
molecular_function GO:0004351 glutamate decarboxylase activity
molecular_function GO:0030170 pyridoxal phosphate binding