Lsi01G020410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G020410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	extradiol ring-cleavage dioxygenase-like
Location	chr01: 28178622 .. 28178870 (-)
RNA-Seq Expression	Lsi01G020410
Synteny	Lsi01G020410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTGAATGACACTTTCTACGTATCCCATGGATCTCCCACCCTAACCATCGACGACACCATCAAAGCAAGACATTTCTTCCAATCTTGGAAGGAGAAGGTTTTCCCCCAAAGACCTAAAGCCATCCTCTGCATTTCTGCCCATTACGACACCGCCTACCCTATGGTCAATGTCGTTTCAGGCCTCAACGACACCATCTACGACTTCTATGGCTTCCCTTCGGAAATGTACAAGGTAACCATGTAG

mRNA sequence

ATGGCTTTGAATGACACTTTCTACGTATCCCATGGATCTCCCACCCTAACCATCGACGACACCATCAAAGCAAGACATTTCTTCCAATCTTGGAAGGAGAAGGTTTTCCCCCAAAGACCTAAAGCCATCCTCTGCATTTCTGCCCATTACGACACCGCCTACCCTATGGTCAATGTCGTTTCAGGCCTCAACGACACCATCTACGACTTCTATGGCTTCCCTTCGGAAATGTACAAGGTAACCATGTAG

Coding sequence (CDS)

ATGGCTTTGAATGACACTTTCTACGTATCCCATGGATCTCCCACCCTAACCATCGACGACACCATCAAAGCAAGACATTTCTTCCAATCTTGGAAGGAGAAGGTTTTCCCCCAAAGACCTAAAGCCATCCTCTGCATTTCTGCCCATTACGACACCGCCTACCCTATGGTCAATGTCGTTTCAGGCCTCAACGACACCATCTACGACTTCTATGGCTTCCCTTCGGAAATGTACAAGGTAACCATGTAG

Protein sequence

MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSGLNDTIYDFYGFPSEMYKVTM

Homology

[Close]

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot
Match: Q949R4 (Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 4.4e-23
Identity = 48/78 (61.54%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          +N TF++SHGSPTL+IDD+++AR FF+SW +KV PQ+PK+IL ISAH+DT +P VN V  
Sbjct: 4  VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVL- 63

Query: 63 LNDTIYDFYGFPSEMYKV 81
           N+TI+DF GFP  MYK+
Sbjct: 64 RNNTIHDFSGFPDPMYKL 80

[Close]

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot
Match: Q70FG7 (4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.5e-20
Identity = 41/77 (53.25%), Postives = 61/77 (79.22%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          + +TF++SHG+P + IDD+  ++ F +SW+EK+F ++PKAIL ISAH++T  P VNVV  
Sbjct: 7  IKETFFISHGTPMMAIDDSKPSKKFLESWREKIFSKKPKAILVISAHWETDQPSVNVVD- 66

Query: 63 LNDTIYDFYGFPSEMYK 80
          +NDTIYDF GFP+ +Y+
Sbjct: 67 INDTIYDFRGFPARLYQ 82

[Close]

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot
Match: I3PFJ9 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.3e-18
Identity = 37/77 (48.05%), Postives = 59/77 (76.62%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          + ++F+++HG+P LT++DT   R FF++W+EK+F ++PKAIL IS H++T  P VN V  
Sbjct: 14 IKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILIISGHWETVKPTVNAVH- 73

Query: 63 LNDTIYDFYGFPSEMYK 80
          +NDTI+DF  +P+ MY+
Sbjct: 74 INDTIHDFDDYPAAMYQ 89

[Close]

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot
Match: I3PFJ3 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.2e-17
Identity = 37/76 (48.68%), Postives = 58/76 (76.32%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          + ++F+++HG+P LT++DT   R FF++W+EK+F ++PKAIL IS H++T  P VN V  
Sbjct: 14 IKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILIISGHWETVKPTVNAVH- 73

Query: 63 LNDTIYDFYGFPSEMY 79
          +NDTI+DF  +P+ MY
Sbjct: 74 INDTIHDFDDYPAAMY 88

[Close]

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot
Match: B6F0W8 (4,5-DOPA dioxygenase extradiol OS=Mirabilis jalapa OX=3538 GN=DOD PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.4e-13
Identity = 33/78 (42.31%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          +  T+Y++HG P + +   IK R F + W+E V  ++PK+IL ISAH+DT  P VN V  
Sbjct: 1  MKGTYYINHGDPLMYLKKHIKLRQFLEGWQENVVIEKPKSILIISAHWDTNVPTVNFVEH 60

Query: 63 LNDTIYDFYGFPSEMYKV 81
            DTI+DF  +P  +Y++
Sbjct: 61 C-DTIHDFDDYPDPLYQI 77

[Close]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL
Match: A0A6J1HS49 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467292 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.2e-33
Identity = 68/80 (85.00%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MALNDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 1  MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MY++
Sbjct: 61 SGPNDTIYDFYGFPSSMYEL 80

[Close]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL
Match: A0A6J1F3T4 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111439883 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.4e-33
Identity = 67/80 (83.75%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MA NDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 1  MAFNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MY++
Sbjct: 61 SGPNDTIYDFYGFPSSMYEL 80

[Close]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL
Match: A0A6J1D7R1 (extradiol ring-cleavage dioxygenase OS=Momordica charantia OX=3673 GN=LOC111017797 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-32
Identity = 64/80 (80.00%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MA++DTFY+SHGSPTL+IDDTI+AR FFQSWK+KVFPQ+PK+ILCISAHYDT++P +NVV
Sbjct: 1  MAVSDTFYISHGSPTLSIDDTIEARQFFQSWKQKVFPQKPKSILCISAHYDTSFPTLNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MYK+
Sbjct: 61 SGPNDTIYDFYGFPSSMYKL 80

[Close]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL
Match: A0A6J1HWD7 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467255 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-30
Identity = 63/80 (78.75%), Postives = 71/80 (88.75%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MALNDTFY+SHGSPTL+IDD+IKAR FF+SWK +V   +PKAILCISAHYDT +P VNVV
Sbjct: 1  MALNDTFYLSHGSPTLSIDDSIKARQFFKSWKNEVVAVKPKAILCISAHYDTTFPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MYK+
Sbjct: 61 SGPNDTIYDFYGFPSSMYKI 80

[Close]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL
Match: A0A6J1EYV3 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111439882 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-29
Identity = 62/80 (77.50%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MALNDTFY+SHGSP L+IDD+IKAR FF+SWK +V   +PKAILCISAHYDT +P VNVV
Sbjct: 1  MALNDTFYLSHGSPMLSIDDSIKARQFFKSWKNEVVAVKPKAILCISAHYDTTFPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MYK+
Sbjct: 61 SGPNDTIYDFYGFPSSMYKI 80

[Close]

BLAST of Lsi01G020410 vs. NCBI nr
Match: XP_038883407.1 (extradiol ring-cleavage dioxygenase-like [Benincasa hispida])

HSP 1 Score: 158.3 bits (399), Expect = 2.8e-35
Identity = 71/80 (88.75%), Postives = 76/80 (95.00%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MAL DTFYVSHGSPTLTIDDTIKAR FFQSWKEK+FPQRPKAILCISAHYDT+YP +NVV
Sbjct: 1  MALKDTFYVSHGSPTLTIDDTIKARQFFQSWKEKIFPQRPKAILCISAHYDTSYPTINVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS+MYK+
Sbjct: 61 SGPNDTIYDFYGFPSKMYKL 80

[Close]

BLAST of Lsi01G020410 vs. NCBI nr
Match: KAG6603159.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 151.0 bits (380), Expect = 4.5e-33
Identity = 68/80 (85.00%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MALNDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 1  MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MY++
Sbjct: 61 SGPNDTIYDFYGFPSSMYEL 80

[Close]

BLAST of Lsi01G020410 vs. NCBI nr
Match: KAG7033478.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.0 bits (380), Expect = 4.5e-33
Identity = 68/80 (85.00%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 1   MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
           MALNDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 142 MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 201

Query: 61  SGLNDTIYDFYGFPSEMYKV 81
           SG NDTIYDFYGFPS MY++
Sbjct: 202 SGPNDTIYDFYGFPSSMYEL 221

[Close]

BLAST of Lsi01G020410 vs. NCBI nr
Match: XP_022967922.1 (extradiol ring-cleavage dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 151.0 bits (380), Expect = 4.5e-33
Identity = 68/80 (85.00%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MALNDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 1  MALNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MY++
Sbjct: 61 SGPNDTIYDFYGFPSSMYEL 80

[Close]

BLAST of Lsi01G020410 vs. NCBI nr
Match: XP_022933118.1 (extradiol ring-cleavage dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 149.4 bits (376), Expect = 1.3e-32
Identity = 67/80 (83.75%), Postives = 73/80 (91.25%), Query Frame = 0


Query: 1  MALNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVV 60
          MA NDTFYVSHGSPTLTIDD IKARHFF+SWK+KVF Q PKAILC+SAH+DTAYP VNVV
Sbjct: 1  MAFNDTFYVSHGSPTLTIDDGIKARHFFKSWKDKVFSQTPKAILCVSAHFDTAYPTVNVV 60

Query: 61 SGLNDTIYDFYGFPSEMYKV 81
          SG NDTIYDFYGFPS MY++
Sbjct: 61 SGPNDTIYDFYGFPSSMYEL 80

[Close]

BLAST of Lsi01G020410 vs. TAIR 10
Match: AT4G15093.1 (catalytic LigB subunit of aromatic ring-opening dioxygenase family )

HSP 1 Score: 108.2 bits (269), Expect = 3.1e-24
Identity = 48/78 (61.54%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 3  LNDTFYVSHGSPTLTIDDTIKARHFFQSWKEKVFPQRPKAILCISAHYDTAYPMVNVVSG 62
          +N TF++SHGSPTL+IDD+++AR FF+SW +KV PQ+PK+IL ISAH+DT +P VN V  
Sbjct: 4  VNQTFFLSHGSPTLSIDDSLEARQFFKSWTQKVLPQKPKSILVISAHWDTKFPSVNTVL- 63

Query: 63 LNDTIYDFYGFPSEMYKV 81
           N+TI+DF GFP  MYK+
Sbjct: 64 RNNTIHDFSGFPDPMYKL 80

The following BLAST results are available for this feature:

BLAST of Lsi01G020410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949R4	4.4e-23	61.54	Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2...	[more]
Q70FG7	3.5e-20	53.25	4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1	[more]
I3PFJ9	7.3e-18	48.05	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
I3PFJ3	1.2e-17	48.68	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
B6F0W8	2.4e-13	42.31	4,5-DOPA dioxygenase extradiol OS=Mirabilis jalapa OX=3538 GN=DOD PE=1 SV=1	[more]

BLAST of Lsi01G020410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HS49	2.2e-33	85.00	extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1F3T4	6.4e-33	83.75	extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1D7R1	1.4e-32	80.00	extradiol ring-cleavage dioxygenase OS=Momordica charantia OX=3673 GN=LOC1110177...	[more]
A0A6J1HWD7	2.3e-30	78.75	extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1EYV3	1.1e-29	77.50	extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Lsi01G020410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883407.1	2.8e-35	88.75	extradiol ring-cleavage dioxygenase-like [Benincasa hispida]	[more]
KAG6603159.1	4.5e-33	85.00	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG7033478.1	4.5e-33	85.00	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_022967922.1	4.5e-33	85.00	extradiol ring-cleavage dioxygenase-like [Cucurbita maxima]	[more]
XP_022933118.1	1.3e-32	83.75	extradiol ring-cleavage dioxygenase-like [Cucurbita moschata]	[more]

BLAST of Lsi01G020410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15093.1	3.1e-24	61.54	catalytic LigB subunit of aromatic ring-opening dioxygenase family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.830.10		coord: 1..82 e-value: 1.6E-20 score: 75.1
None	No IPR available	PANTHER	PTHR30096:SF18	EXTRADIOL RING-CLEAVAGE DIOXYGENASE-LIKE	coord: 3..80
None	No IPR available	PANTHER	PTHR30096	UNCHARACTERIZED	coord: 3..80
None	No IPR available	SUPERFAMILY	53213	LigB-like	coord: 6..81
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	PFAM	PF02900	LigB	coord: 6..75 e-value: 7.2E-8 score: 31.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020410.1	Lsi01G020410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006725	cellular aromatic compound metabolic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0016701	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016491	oxidoreductase activity