Lsi01G020400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G020400
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	extradiol ring-cleavage dioxygenase-like
Location	chr01: 28177684 .. 28178580 (-)
RNA-Seq Expression	Lsi01G020400
Synteny	Lsi01G020400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTTAAATATATACAGTGAGCATGTATCAGTCAAAATATCCGGGCCCGAGAGATCCGACATTGGTAAAGAGGGTGAAGGAAGCTCTGGCGGGTGGAGGAGGAGAAGGGGCGGGGGTTGGATCATGGAGCATGGGTGCCTTTGATGTTCATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGTACCTCTAAAGGATGAAGGAGTTCTTATTTTTGGCTCAGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGGTAACTTCATTAGAATGTTTTTTTTTAATTTTATATTTTGTTTAATTTTTGTGCTATATATGGTAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAAATCACCATCTCCTATATACATCTTTCATTTCTTTTCAATTTTCTTCATCCAAAATGCTCCTCTTTATTTCTCTCACATACCATTGATTTTATTTGCTTTTTCTTTATTACAAACTCTCTTTATGAGTTTGATTTTGTTGTTAATAATAATGAAATCAAAACAATTATATTCTTCGTACCTACGTCTATTCTACAATGCTTTCAAATATACTACAATAGCTATATGTGTGTGTATATATATAGACTC

mRNA sequence

ATGAAATCAAAATATCCGGGCCCGAGAGATCCGACATTGGTAAAGAGGGTGAAGGAAGCTCTGGCGGGTGGAGGAGGAGAAGGGGCGGGGGTTGGATCATGGAGCATGGGTGCCTTTGATGTTCATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAAATCACCATCTCCTATATACATCTTTCATTTCTTTTCAATTTTCTTCATCCAAAATGCTCCTCTTTATTTCTCTCACATACCATTGATTTTATTTGCTTTTTCTTTATTACAAACTCTCTTTATGAGTTTGATTTTGTTGTTAATAATAATGAAATCAAAACAATTATATTCTTCGTACCTACGTCTATTCTACAATGCTTTCAAATATACTACAATAGCTATATGTGTGTGTATATATATAGACTC

Coding sequence (CDS)

ATGAAATCAAAATATCCGGGCCCGAGAGATCCGACATTGGTAAAGAGGGTGAAGGAAGCTCTGGCGGGTGGAGGAGGAGAAGGGGCGGGGGTTGGATCATGGAGCATGGGTGCCTTTGATGTTCATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAA

Protein sequence

MKSKYPGPRDPTLVKRVKEALAGGGGEGAGVGSWSMGAFDVHVSRGRHSCLPTLSTNTPKWDTPLQHGEGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYASYQFTTL

Homology

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BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot
Match: Q949R4 (Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 8.8e-34
Identity = 87/198 (43.94%), Postives = 106/198 (53.54%), Query Frame = 0


Query: 2   KSKYPGPRDPTLVKRVKEALAGGGGEGAGVGSWSMGAFDVHVSRG-RHSCLPTLSTNTPK 61
           K KY  P    L KRVKE L   GG         M   D    RG  H     L    P+
Sbjct: 79  KLKYEAPGAIELGKRVKELLMKEGG---------MKRVDEDTKRGLDHGAWVPLMLMYPE 138

Query: 62  WDTPL--------QHGE--------------------GFGSATHNLRTLDFDAKGRANAS 121
            D P+        Q+G                     G GSATHNLR LDF+    +   
Sbjct: 139 ADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDGSPVP 198

Query: 122 WAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLI 171
           WA++FD WL+++LLQGRY DVNE+E+KAPN KMAHP P+  +PLHV +GAAGG  KA+ I
Sbjct: 199 WALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGAAGGDAKAEQI 258

[Close]

BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot
Match: Q7XA48 (4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 8.0e-27
Identity = 80/189 (42.33%), Postives = 104/189 (55.03%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGGGEGAGV--------GSW---SMGAFDVHVSRGRHSCLP 63
           KYP P  P L KRV+E L  GG + A +         SW   SM   +  +   + S  P
Sbjct: 86  KYPAPGCPKLAKRVQELLIAGGFKSAKLDEERGFDHSSWVPLSMMCPEADIPVCQLSVQP 145

Query: 64  TLST----NTPKWDTPLQHGEGF-----GSATHNLRTLDFDAKGRANASWAIDFDKWLQE 123
            L      N  +   PL+ GEG      G A H          G   A WA +FD+WL++
Sbjct: 146 GLDATHHFNVGRALAPLK-GEGVLFIGSGGAVHPSDDTPHWFDG--VAPWAAEFDQWLED 205

Query: 124 ALLQGRYEDVNEYEKKAPNP-KMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMS 172
           ALL+GRYEDVN Y+ KAP   K+AHP P+ F PLHVA+GA G   KA+LI+ +WD GT+ 
Sbjct: 206 ALLEGRYEDVNNYQTKAPEGWKLAHPIPEHFLPLHVAMGAGGEKSKAELIYRTWDHGTLG 265

[Close]

BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot
Match: I3PFJ9 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.0e-26
Identity = 76/186 (40.86%), Postives = 102/186 (54.84%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGGGE--------GAGVGSW---SMGAFDVHVSRGRHSCLP 63
           KYP P +P L ++V+E L   G E        G   G+W    +   +  +   + S  P
Sbjct: 91  KYPAPGEPELARKVEEILKKSGFETAETDQKRGLDHGAWVPLMLMYPEADIPVCQLSVQP 150

Query: 64  ----TLSTNTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  +   PL++      G GSATH L        G   A WA  FD WL++A
Sbjct: 151 HLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPHYFDG--VAPWAAAFDSWLRKA 210

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 171
           L+ GR+E+VN YE KAPN K+AHP P+ F+PLHV +GAAG   KA+LIH SWD GT+ + 
Sbjct: 211 LINGRFEEVNIYESKAPNWKLAHPFPEHFYPLHVVLGAAGEKWKAELIHSSWDHGTLCHG 270

[Close]

BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot
Match: I3PFJ3 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 76/186 (40.86%), Postives = 101/186 (54.30%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGGGE--------GAGVGSW---SMGAFDVHVSRGRHSCLP 63
           KYP P  P L ++V+E L   G E        G   G+W    +   +  +   + S  P
Sbjct: 91  KYPAPGAPELARKVEEILKKSGFETAETDEKRGLDHGAWVPLMLMYPEADIPVCQLSVQP 150

Query: 64  ----TLSTNTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  +   PL++      G GSATH L        G   A WA  FD WL++A
Sbjct: 151 HLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPHYFDG--VAPWAAAFDSWLRKA 210

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 171
           L+ GR+E+VN YE KAPN K+AHP P+ F+PLHV +GAAG   KA+LIH SWD GT+ + 
Sbjct: 211 LINGRFEEVNIYETKAPNWKLAHPFPEHFYPLHVVLGAAGEKWKAELIHSSWDHGTLCHG 270

[Close]

BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot
Match: Q70FG7 (4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.0e-25
Identity = 72/186 (38.71%), Postives = 95/186 (51.08%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGGGEGAGVG---SWSMGAFDVHVSRGRHSCLPTLSTNTPK 63
           KY  P  P L  R+++ LAG G +            GA+   +     + +P    +   
Sbjct: 84  KYSAPGSPELANRIQDLLAGSGFKSVNTDKKRGLDHGAWVPLMLMYPEADIPVCQLSVQS 143

Query: 64  WDTPLQHGE----------------GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
                 H +                G GSATH        + G   A WA  FD WL+ A
Sbjct: 144 HLDGTHHYKLGQALAPLKDEGVLIIGSGSATHPSNGTPPCSDG--VAPWAAAFDSWLETA 203

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 171
           L  G YE+VN+YE KAPN K+AHP P+ F+PLHVA+GAAG + KA+LIH+SWD G MSY 
Sbjct: 204 LTNGSYEEVNKYETKAPNWKLAHPWPEHFYPLHVAMGAAGENSKAELIHNSWDGGIMSYG 263

[Close]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL
Match: A0A6J1F3T4 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111439883 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 6.1e-46
Identity = 108/185 (58.38%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  P L KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 81  KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 140

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 141 RLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 200

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPKAKL+HHSWDLGTMSYA
Sbjct: 201 LLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKAKLVHHSWDLGTMSYA 260

[Close]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL
Match: A0A6J1HS49 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467292 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.6e-44
Identity = 106/185 (57.30%), Postives = 118/185 (63.78%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  P L KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 81  KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 140

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 141 RLNGTHHYNMGKALAPLKDEGVLVVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 200

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQ RYEDVNEY+ KAPN + AHP+PD  FPLHVAIGAAGGHPKAKL+HHSWDLGTMSYA
Sbjct: 201 LLQRRYEDVNEYKNKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKAKLVHHSWDLGTMSYA 260

[Close]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL
Match: A0A5A7TUJ3 (Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold30G001920 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.3e-43
Identity = 104/187 (55.61%), Postives = 120/187 (64.17%), Query Frame = 0


Query: 2   KSKYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--G 61
           K KYP P  P L K VKEAL   G        G G   G+W    F     D+ V +   
Sbjct: 79  KLKYPAPGAPALAKSVKEALVRAGFERVEEERGRGLDHGAWVPLMFMYPEADIPVCQLSV 138

Query: 62  RHSCLPTLSTNTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQ 121
           +     T   N  K   PL+       G GSATHNLRTL+ +    A A WA++FD WL+
Sbjct: 139 QSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSSAIAPWALEFDNWLK 198

Query: 122 EALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMS 170
           +ALLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PKAKLIHHSWDLGTMS
Sbjct: 199 DALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPKAKLIHHSWDLGTMS 258

[Close]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL
Match: A0A1S3BM49 (extradiol ring-cleavage dioxygenase OS=Cucumis melo OX=3656 GN=LOC103491530 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.3e-43
Identity = 104/187 (55.61%), Postives = 120/187 (64.17%), Query Frame = 0


Query: 2   KSKYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--G 61
           K KYP P  P L K VKEAL   G        G G   G+W    F     D+ V +   
Sbjct: 79  KLKYPAPGAPALAKSVKEALVRAGFERVEEERGRGLDHGAWVPLMFMYPEADIPVCQLSV 138

Query: 62  RHSCLPTLSTNTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQ 121
           +     T   N  K   PL+       G GSATHNLRTL+ +    A A WA++FD WL+
Sbjct: 139 QSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSSAIAPWALEFDNWLK 198

Query: 122 EALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMS 170
           +ALLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PKAKLIHHSWDLGTMS
Sbjct: 199 DALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPKAKLIHHSWDLGTMS 258

[Close]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL
Match: A0A5D3DUJ2 (Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G00370 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.4e-42
Identity = 103/185 (55.68%), Postives = 119/185 (64.32%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  P L K VKEAL   G        G G   G+W    F     D+ V +   + 
Sbjct: 79  KYPAPGAPALAKSVKEALVRAGFERVEEERGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 138

Query: 64  SCLPTLSTNTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTL+ +    A A WA++FD WL++A
Sbjct: 139 HLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSSAIAPWALEFDNWLKDA 198

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PKAKLIHHSWDLGTMSYA
Sbjct: 199 LLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPKAKLIHHSWDLGTMSYA 258

[Close]

BLAST of Lsi01G020400 vs. NCBI nr
Match: XP_038883407.1 (extradiol ring-cleavage dioxygenase-like [Benincasa hispida])

HSP 1 Score: 213.0 bits (541), Expect = 2.0e-51
Identity = 118/188 (62.77%), Postives = 126/188 (67.02%), Query Frame = 0


Query: 2   KSKYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAFDVHVSRGRHSCLPT 61
           K KYP P   TL KRVKEAL G G        G G   G+W    F ++       C  +
Sbjct: 79  KLKYPAPGGATLAKRVKEALIGAGFERVDEEKGRGLDHGAWVPLMF-MYPEADILVCQLS 138

Query: 62  LST--------NTPKWDTPLQHGE----GFGSATHNLRTLDFDAKGRANASWAIDFDKWL 121
           + T        N  K   PL+       G GSATHNLRTLDFDA+G ANASWAIDFDKWL
Sbjct: 139 VQTRQNGIHHYNMGKALAPLKDEGVLIFGSGSATHNLRTLDFDARGSANASWAIDFDKWL 198

Query: 122 QEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTM 170
           Q+ALLQGRYEDVNEYEKKAP  KM HPNPD FFPLHVAIGAAGG PKAKLIHHSWDLGTM
Sbjct: 199 QQALLQGRYEDVNEYEKKAPQAKMTHPNPDHFFPLHVAIGAAGGQPKAKLIHHSWDLGTM 258

[Close]

BLAST of Lsi01G020400 vs. NCBI nr
Match: KAG6603159.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 194.1 bits (492), Expect = 9.7e-46
Identity = 108/185 (58.38%), Postives = 121/185 (65.41%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  PTL KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 81  KYPAPGAPTLAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 140

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 141 RLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 200

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRY+DVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPKAKL+HHSWDLGTMSYA
Sbjct: 201 LLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKAKLVHHSWDLGTMSYA 260

[Close]

BLAST of Lsi01G020400 vs. NCBI nr
Match: KAG7033478.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 194.1 bits (492), Expect = 9.7e-46
Identity = 108/185 (58.38%), Postives = 121/185 (65.41%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  PTL KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 222 KYPAPGAPTLAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 281

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 282 RLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 341

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRY+DVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPKAKL+HHSWDLGTMSYA
Sbjct: 342 LLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKAKLVHHSWDLGTMSYA 401

[Close]

BLAST of Lsi01G020400 vs. NCBI nr
Match: XP_022933118.1 (extradiol ring-cleavage dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-45
Identity = 108/185 (58.38%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  P L KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 81  KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 140

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 141 RLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 200

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPKAKL+HHSWDLGTMSYA
Sbjct: 201 LLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKAKLVHHSWDLGTMSYA 260

[Close]

BLAST of Lsi01G020400 vs. NCBI nr
Match: XP_023544929.1 (extradiol ring-cleavage dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 191.8 bits (486), Expect = 4.8e-45
Identity = 107/185 (57.84%), Postives = 119/185 (64.32%), Query Frame = 0


Query: 4   KYPGPRDPTLVKRVKEALAGGG--------GEGAGVGSWSMGAF-----DVHVSR--GRH 63
           KYP P  P L KRVKEAL G G        G G   G+W    F     D+ V +   + 
Sbjct: 81  KYPAPGAPALAKRVKEALMGAGFERVDEEKGRGLDHGAWVPLMFMYPEADIPVCQLSVQS 140

Query: 64  SCLPTLSTNTPKWDTPLQHG----EGFGSATHNLRTLDFDAKGRANASWAIDFDKWLQEA 123
               T   N  K   PL+       G GSATHNLRTLD  A+   NASWAI+FD WL+ A
Sbjct: 141 RLNGTHHYNMGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSSVNASWAIEFDDWLKHA 200

Query: 124 LLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYA 170
           LLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK KL+HHSWDLGTMSYA
Sbjct: 201 LLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPKTKLVHHSWDLGTMSYA 260

[Close]

BLAST of Lsi01G020400 vs. TAIR 10
Match: AT4G15093.1 (catalytic LigB subunit of aromatic ring-opening dioxygenase family )

HSP 1 Score: 144.8 bits (364), Expect = 6.3e-35
Identity = 87/198 (43.94%), Postives = 106/198 (53.54%), Query Frame = 0


Query: 2   KSKYPGPRDPTLVKRVKEALAGGGGEGAGVGSWSMGAFDVHVSRG-RHSCLPTLSTNTPK 61
           K KY  P    L KRVKE L   GG         M   D    RG  H     L    P+
Sbjct: 79  KLKYEAPGAIELGKRVKELLMKEGG---------MKRVDEDTKRGLDHGAWVPLMLMYPE 138

Query: 62  WDTPL--------QHGE--------------------GFGSATHNLRTLDFDAKGRANAS 121
            D P+        Q+G                     G GSATHNLR LDF+    +   
Sbjct: 139 ADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDGSPVP 198

Query: 122 WAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLI 171
           WA++FD WL+++LLQGRY DVNE+E+KAPN KMAHP P+  +PLHV +GAAGG  KA+ I
Sbjct: 199 WALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGAAGGDAKAEQI 258

The following BLAST results are available for this feature:

BLAST of Lsi01G020400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949R4	8.8e-34	43.94	Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2...	[more]
Q7XA48	8.0e-27	42.33	4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=...	[more]
I3PFJ9	4.0e-26	40.86	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
I3PFJ3	1.5e-25	40.86	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
Q70FG7	2.0e-25	38.71	4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1	[more]

BLAST of Lsi01G020400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3T4	6.1e-46	58.38	extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1HS49	2.6e-44	57.30	extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A5A7TUJ3	6.3e-43	55.61	Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A1S3BM49	6.3e-43	55.61	extradiol ring-cleavage dioxygenase OS=Cucumis melo OX=3656 GN=LOC103491530 PE=3...	[more]
A0A5D3DUJ2	1.4e-42	55.68	Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of Lsi01G020400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883407.1	2.0e-51	62.77	extradiol ring-cleavage dioxygenase-like [Benincasa hispida]	[more]
KAG6603159.1	9.7e-46	58.38	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG7033478.1	9.7e-46	58.38	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_022933118.1	1.3e-45	58.38	extradiol ring-cleavage dioxygenase-like [Cucurbita moschata]	[more]
XP_023544929.1	4.8e-45	57.84	extradiol ring-cleavage dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi01G020400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15093.1	6.3e-35	43.94	catalytic LigB subunit of aromatic ring-opening dioxygenase family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.830.10		coord: 70..169 e-value: 1.3E-19 score: 72.1
None	No IPR available	PANTHER	PTHR30096:SF14	AROMATIC RING-OPENING DIOXYGENASE, CATALYTIC PROTEIN	coord: 70..170
None	No IPR available	PANTHER	PTHR30096	UNCHARACTERIZED	coord: 70..170
None	No IPR available	SUPERFAMILY	53213	LigB-like	coord: 4..168
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	PFAM	PF02900	LigB	coord: 72..168 e-value: 7.6E-14 score: 51.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020400.1	Lsi01G020400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006725	cellular aromatic compound metabolic process
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0016491	oxidoreductase activity