Lsi01G020390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G020390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	extradiol ring-cleavage dioxygenase-like
Location	chr01: 28175679 .. 28176176 (+)
RNA-Seq Expression	Lsi01G020390
Synteny	Lsi01G020390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGTACCTCTAAAGGATGAAGGAGTTCTTATTTTTGGCTCAGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGGTAACTTCATTAGAATGTTTTTTTTTAATTTTATATTTTGTTTAATTTTTGTGCTATATATGGTAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAA

mRNA sequence

ATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGTACCTCTAAAGGATGAAGGAGTTCTTATTTTTGGCTCAGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAA

Coding sequence (CDS)

ATGTATCCAGAGGCAGACATTCCTGTTTGCCAACTCTCAGTACAAACACACCCAAATGGGACACACCATTACAACATGGGGAAGGCTTTGGTACCTCTAAAGGATGAAGGAGTTCTTATTTTTGGCTCAGGAAGTGCAACACACAACCTCAGAACACTCGACTTTGATGCCAAAGGACGTGCCAACGCCTCGTGGGCTATTGATTTCGATAAATGGCTTCAAGAAGCTCTCCTTCAAGGAAGATATGAAGATGTGAATGAATATGAGAAGAAGGCTCCAAATCCAAAAATGGCACATCCCAATCCAGATCAGTTTTTTCCTCTCCATGTTGCAATTGGGGCTGCCGGAGGACACCCAAAAGCAAAGCTAATCCACCATAGTTGGGACCTTGGCACCATGTCCTATGCCTCCTACCAGTTCACTACCCTTTAA

Protein sequence

MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGRANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPKAKLIHHSWDLGTMSYASYQFTTL

Homology

[Close]

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot
Match: Q949R4 (Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.7e-54
Identity = 96/142 (67.61%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIP+CQLSVQ++ NG++HYNMGKAL  LKDEGVLI GSGSATHNLR LDF+    
Sbjct: 126 MYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDG 185

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           +   WA++FD WL+++LLQGRY DVNE+E+KAPN KMAHP P+  +PLHV +GAAGG  K
Sbjct: 186 SPVPWALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGAAGGDAK 245

Query: 121 AKLIHHSWDLGTMSYASYQFTT 143
           A+ IH SW LGT+SY+SY FT+
Sbjct: 246 AEQIHTSWQLGTLSYSSYSFTS 267

[Close]

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot
Match: Q70FG7 (4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 6.9e-48
Identity = 92/142 (64.79%), Postives = 109/142 (76.76%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+H +GTHHY +G+AL PLKDEGVLI GSGSATH        + G 
Sbjct: 128 MYPEADIPVCQLSVQSHLDGTHHYKLGQALAPLKDEGVLIIGSGSATHPSNGTPPCSDG- 187

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
             A WA  FD WL+ AL  G YE+VN+YE KAPN K+AHP P+ F+PLHVA+GAAG + K
Sbjct: 188 -VAPWAAAFDSWLETALTNGSYEEVNKYETKAPNWKLAHPWPEHFYPLHVAMGAAGENSK 247

Query: 121 AKLIHHSWDLGTMSYASYQFTT 143
           A+LIH+SWD G MSY SY+FT+
Sbjct: 248 AELIHNSWDGGIMSYGSYKFTS 267

[Close]

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot
Match: I3PFJ9 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 4.9e-46
Identity = 88/142 (61.97%), Postives = 108/142 (76.06%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ H +GT+HYN+G+AL PLK++GVLI GSGSATH L        G 
Sbjct: 135 MYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPHYFDG- 194

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
             A WA  FD WL++AL+ GR+E+VN YE KAPN K+AHP P+ F+PLHV +GAAG   K
Sbjct: 195 -VAPWAAAFDSWLRKALINGRFEEVNIYESKAPNWKLAHPFPEHFYPLHVVLGAAGEKWK 254

Query: 121 AKLIHHSWDLGTMSYASYQFTT 143
           A+LIH SWD GT+ + SY+FT+
Sbjct: 255 AELIHSSWDHGTLCHGSYKFTS 274

[Close]

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot
Match: I3PFJ3 (4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 6.5e-46
Identity = 88/142 (61.97%), Postives = 108/142 (76.06%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ H +GT+HYN+G+AL PLK++GVLI GSGSATH L        G 
Sbjct: 135 MYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLIIGSGSATHPLDETPHYFDG- 194

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
             A WA  FD WL++AL+ GR+E+VN YE KAPN K+AHP P+ F+PLHV +GAAG   K
Sbjct: 195 -VAPWAAAFDSWLRKALINGRFEEVNIYETKAPNWKLAHPFPEHFYPLHVVLGAAGEKWK 254

Query: 121 AKLIHHSWDLGTMSYASYQFTT 143
           A+LIH SWD GT+ + SY+FT+
Sbjct: 255 AELIHSSWDHGTLCHGSYKFTS 274

[Close]

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot
Match: Q7XA48 (4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-41
Identity = 83/144 (57.64%), Postives = 103/144 (71.53%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           M PEADIPVCQLSVQ   + THH+N+G+AL PLK EGVL  GSG A H          G 
Sbjct: 130 MCPEADIPVCQLSVQPGLDATHHFNVGRALAPLKGEGVLFIGSGGAVHPSDDTPHWFDG- 189

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNP-KMAHPNPDQFFPLHVAIGAAGGHP 120
             A WA +FD+WL++ALL+GRYEDVN Y+ KAP   K+AHP P+ F PLHVA+GA G   
Sbjct: 190 -VAPWAAEFDQWLEDALLEGRYEDVNNYQTKAPEGWKLAHPIPEHFLPLHVAMGAGGEKS 249

Query: 121 KAKLIHHSWDLGTMSYASYQFTTL 144
           KA+LI+ +WD GT+ YASY+FT++
Sbjct: 250 KAELIYRTWDHGTLGYASYKFTSI 271

[Close]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL
Match: A0A6J1F3T4 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111439883 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.9e-64
Identity = 117/141 (82.98%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTLD  A+  
Sbjct: 125 MYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 185 VNASWAIEFDDWLKHALLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKL+HHSWDLGTMSYASYQFT
Sbjct: 245 AKLVHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL
Match: A0A5A7TUJ3 (Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold30G001920 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.1e-64
Identity = 115/141 (81.56%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+H NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTL+ +    
Sbjct: 125 MYPEADIPVCQLSVQSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           A A WA++FD WL++ALLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PK
Sbjct: 185 AIAPWALEFDNWLKDALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKLIHHSWDLGTMSYASYQFT
Sbjct: 245 AKLIHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL
Match: A0A5D3DUJ2 (Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G00370 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.1e-64
Identity = 115/141 (81.56%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+H NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTL+ +    
Sbjct: 123 MYPEADIPVCQLSVQSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSS 182

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           A A WA++FD WL++ALLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PK
Sbjct: 183 AIAPWALEFDNWLKDALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPK 242

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKLIHHSWDLGTMSYASYQFT
Sbjct: 243 AKLIHHSWDLGTMSYASYQFT 263

[Close]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL
Match: A0A1S3BM49 (extradiol ring-cleavage dioxygenase OS=Cucumis melo OX=3656 GN=LOC103491530 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.1e-64
Identity = 115/141 (81.56%), Postives = 128/141 (90.78%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+H NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTL+ +    
Sbjct: 125 MYPEADIPVCQLSVQSHLNGTHHYNLGKALAPLKDEGVLIIGSGSATHNLRTLNRNGNSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           A A WA++FD WL++ALLQGRY+DVNEYEKKAP+ +MAHP+PD FFPLHVAIGAAGG+PK
Sbjct: 185 AIAPWALEFDNWLKDALLQGRYDDVNEYEKKAPHARMAHPSPDHFFPLHVAIGAAGGNPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKLIHHSWDLGTMSYASYQFT
Sbjct: 245 AKLIHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL
Match: A0A6J1HS49 (extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111467292 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-63
Identity = 115/141 (81.56%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYNMGKAL PLKDEGVL+ GSGSATHNLRTLD  A+  
Sbjct: 125 MYPEADIPVCQLSVQSRLNGTHHYNMGKALAPLKDEGVLVVGSGSATHNLRTLDRHARSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQ RYEDVNEY+ KAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 185 VNASWAIEFDDWLKHALLQRRYEDVNEYKNKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKL+HHSWDLGTMSYASYQFT
Sbjct: 245 AKLVHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. NCBI nr
Match: XP_038883407.1 (extradiol ring-cleavage dioxygenase-like [Benincasa hispida])

HSP 1 Score: 274.6 bits (701), Expect = 4.7e-70
Identity = 128/141 (90.78%), Postives = 130/141 (92.20%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADI VCQLSVQT  NG HHYNMGKAL PLKDEGVLIFGSGSATHNLRTLDFDA+G 
Sbjct: 125 MYPEADILVCQLSVQTRQNGIHHYNMGKALAPLKDEGVLIFGSGSATHNLRTLDFDARGS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           ANASWAIDFDKWLQ+ALLQGRYEDVNEYEKKAP  KM HPNPD FFPLHVAIGAAGG PK
Sbjct: 185 ANASWAIDFDKWLQQALLQGRYEDVNEYEKKAPQAKMTHPNPDHFFPLHVAIGAAGGQPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKLIHHSWDLGTMSYASYQFT
Sbjct: 245 AKLIHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. NCBI nr
Match: XP_023544929.1 (extradiol ring-cleavage dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.0 bits (650), Expect = 3.9e-64
Identity = 117/141 (82.98%), Postives = 125/141 (88.65%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYNMGKAL PLKDEGVLI GSGSATHNLRTLD  A+  
Sbjct: 125 MYPEADIPVCQLSVQSRLNGTHHYNMGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 185 VNASWAIEFDDWLKHALLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
            KL+HHSWDLGTMSYASYQFT
Sbjct: 245 TKLVHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. NCBI nr
Match: XP_022933118.1 (extradiol ring-cleavage dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 255.0 bits (650), Expect = 3.9e-64
Identity = 117/141 (82.98%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTLD  A+  
Sbjct: 125 MYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQGRYEDVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 185 VNASWAIEFDDWLKHALLQGRYEDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKL+HHSWDLGTMSYASYQFT
Sbjct: 245 AKLVHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. NCBI nr
Match: KAG6603159.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 116/141 (82.27%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTLD  A+  
Sbjct: 125 MYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSS 184

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQGRY+DVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 185 VNASWAIEFDDWLKHALLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 244

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKL+HHSWDLGTMSYASYQFT
Sbjct: 245 AKLVHHSWDLGTMSYASYQFT 265

[Close]

BLAST of Lsi01G020390 vs. NCBI nr
Match: KAG7033478.1 (Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 253.4 bits (646), Expect = 1.1e-63
Identity = 116/141 (82.27%), Postives = 126/141 (89.36%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIPVCQLSVQ+  NGTHHYN+GKAL PLKDEGVLI GSGSATHNLRTLD  A+  
Sbjct: 266 MYPEADIPVCQLSVQSRLNGTHHYNVGKALAPLKDEGVLIVGSGSATHNLRTLDRHARSS 325

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
            NASWAI+FD WL+ ALLQGRY+DVNEY+KKAPN + AHP+PD  FPLHVAIGAAGGHPK
Sbjct: 326 VNASWAIEFDDWLKHALLQGRYKDVNEYKKKAPNAEKAHPSPDHLFPLHVAIGAAGGHPK 385

Query: 121 AKLIHHSWDLGTMSYASYQFT 142
           AKL+HHSWDLGTMSYASYQFT
Sbjct: 386 AKLVHHSWDLGTMSYASYQFT 406

[Close]

BLAST of Lsi01G020390 vs. TAIR 10
Match: AT4G15093.1 (catalytic LigB subunit of aromatic ring-opening dioxygenase family )

HSP 1 Score: 213.4 bits (542), Expect = 1.2e-55
Identity = 96/142 (67.61%), Postives = 117/142 (82.39%), Query Frame = 0


Query: 1   MYPEADIPVCQLSVQTHPNGTHHYNMGKALVPLKDEGVLIFGSGSATHNLRTLDFDAKGR 60
           MYPEADIP+CQLSVQ++ NG++HYNMGKAL  LKDEGVLI GSGSATHNLR LDF+    
Sbjct: 126 MYPEADIPICQLSVQSNQNGSYHYNMGKALASLKDEGVLIIGSGSATHNLRKLDFNITDG 185

Query: 61  ANASWAIDFDKWLQEALLQGRYEDVNEYEKKAPNPKMAHPNPDQFFPLHVAIGAAGGHPK 120
           +   WA++FD WL+++LLQGRY DVNE+E+KAPN KMAHP P+  +PLHV +GAAGG  K
Sbjct: 186 SPVPWALEFDHWLRDSLLQGRYGDVNEWEEKAPNAKMAHPWPEHLYPLHVVMGAAGGDAK 245

Query: 121 AKLIHHSWDLGTMSYASYQFTT 143
           A+ IH SW LGT+SY+SY FT+
Sbjct: 246 AEQIHTSWQLGTLSYSSYSFTS 267

The following BLAST results are available for this feature:

BLAST of Lsi01G020390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949R4	1.7e-54	67.61	Extradiol ring-cleavage dioxygenase OS=Arabidopsis thaliana OX=3702 GN=LIGB PE=2...	[more]
Q70FG7	6.9e-48	64.79	4,5-DOPA dioxygenase extradiol OS=Beta vulgaris OX=161934 GN=DODA PE=1 SV=1	[more]
I3PFJ9	4.9e-46	61.97	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
I3PFJ3	6.5e-46	61.97	4,5-DOPA dioxygenase extradiol 1 OS=Beta vulgaris OX=161934 GN=DODA1 PE=2 SV=1	[more]
Q7XA48	1.3e-41	57.64	4,5-DOPA dioxygenase extradiol OS=Portulaca grandiflora OX=3583 GN=DODA PE=1 SV=...	[more]

BLAST of Lsi01G020390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3T4	1.9e-64	82.98	extradiol ring-cleavage dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A5A7TUJ3	7.1e-64	81.56	Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A5D3DUJ2	7.1e-64	81.56	Extradiol ring-cleavage dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3BM49	7.1e-64	81.56	extradiol ring-cleavage dioxygenase OS=Cucumis melo OX=3656 GN=LOC103491530 PE=3...	[more]
A0A6J1HS49	2.7e-63	81.56	extradiol ring-cleavage dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]

BLAST of Lsi01G020390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883407.1	4.7e-70	90.78	extradiol ring-cleavage dioxygenase-like [Benincasa hispida]	[more]
XP_023544929.1	3.9e-64	82.98	extradiol ring-cleavage dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022933118.1	3.9e-64	82.98	extradiol ring-cleavage dioxygenase-like [Cucurbita moschata]	[more]
KAG6603159.1	1.1e-63	82.27	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. soro...	[more]
KAG7033478.1	1.1e-63	82.27	Extradiol ring-cleavage dioxygenase, partial [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of Lsi01G020390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15093.1	1.2e-55	67.61	catalytic LigB subunit of aromatic ring-opening dioxygenase family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004183	Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B	PFAM	PF02900	LigB	coord: 1..140 e-value: 5.5E-28 score: 97.8
None	No IPR available	GENE3D	3.40.830.10		coord: 1..141 e-value: 3.9E-39 score: 136.2
None	No IPR available	PANTHER	PTHR30096:SF18	EXTRADIOL RING-CLEAVAGE DIOXYGENASE-LIKE	coord: 1..143
None	No IPR available	PANTHER	PTHR30096	UNCHARACTERIZED	coord: 1..143
None	No IPR available	SUPERFAMILY	53213	LigB-like	coord: 1..140
IPR014436	Extradiol aromatic ring-opening dioxygenase, DODA-type	CDD	cd07363	45_DOPA_Dioxygenase	coord: 1..140 e-value: 5.01332E-56 score: 173.867

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020390.1	Lsi01G020390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006725	cellular aromatic compound metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0050297	stizolobate synthase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016701	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen