Lsi01G020320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G020320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDNA-directed DNA polymerase
Locationchr01: 28019889 .. 28023980 (+)
RNA-Seq ExpressionLsi01G020320
SyntenyLsi01G020320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTATCGACTTGATGGTGCTTGATGTTCTTATATCGCTAAATGCAGGAGTCGATGGAATTGGAAATGTCAATGCTGTGCAACTTATCACTAGATTCGGTGAGTAGGTTTAGGAGACCAATGCCTTCCCCAGAGAATTGTAGACTACAATATATTCAATCTCATGTTTGCTTACCTTCTGTATTTTATTATTATCAGGAATAATGGTTAAGGTGGATGCCTGTCTTCTATAGCTGCCTATTCAATTCAAAGAAATAATAAATCCTATAAATCAGGAGATATTTAAAAAAAAGAAAAAAAAACAATTGTAAGTCGGGAGATAAATTTACCATCCAGATACAAGTCCTAAACTATTGAGGTTATTAATGAGTATGAATCTTCATCTCTTGCATATTTCAAATAAGCGAGAACCAGTAAGATGAATAAGAAAATATTTTGGCAATTTGATTTAAATTTCTCCTGGCATCTTTTGCTGTTTCAACTTGTAATCTTATGTTCAAGATATCCTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTTTTTTTTTTTTTTTTTTGTGTCTCTTTTTGATTTGGTGGAATAAAATATATTCATGAGAAAAATATTTAGAAGAATGGAAATGGAGGAGAAATCCTCTAACTAAAAAATATATACATATATATTTAAAACACTTCCTGTAATGGCTTTGAATAGATTTTGTATTGTGTTTGGAGCTGTGCATGTGTGTGTTTTCTATTTTAAAATTTTTGGAAGTAAAACAAAGTAACAATAAACACTTTCTGCAAAAGTAATCCAAATCTTTTTTTTTTTTTTTTTACCGTTGAAGGTAAAGTTAGAACTGTATTATTTCGAAATAAAATGAGAATGCCATGGTACTCTAGTTTTGTGTTCCTTCATTATATTTCTAATCTTTATTCTTTATTCTGTTGTCTGTGGTGCCTGAAATGTGATGGTTTGTGTGTACTTATTTACAAATTCTCACTGGTCAGGCACGTTAGAAAATTTGTTGCAACATGTTGATCAAGTGGAAGATGAACGTATTAAGAAGGTATGTCTATCAGAGTACAATAACTGGCTTCTGAACCAAAATATTTATAAGAACGCTTGAATTATTTTCAGTGTATTAGAAGGGGTAAGCTTATACAGGTTAGGAATTTTATTAATTTAGTCATGTTGTTCGGTGCTAGACTTGATTGCATTCAGCCTTTTAGATGTCTGGATTGAAGTCAGACGTATAAAACAAATTGTGATAACCAATTGTGTATATAAATTCGACTAAGATTGAGAAGCAATTTTAGAAGTAAACAAGACAAAGAAGTTCTAGTAAACAAACTTATTTTTTCAAAACAAAAACAAAACTATAATCAAACGGGCTTCAGTAAGCATGTTTTGGGTAGAGAGAGAGTTTTTTCTTCTTCTTTCCAATGTAACATGTTAGATGATATAAGATTAAATTTGCCTTCACCCATTAGCTTAAGTTTTTTGGTCAATTGGCAATTTAACATGGTATCAGAGCAGGTTGGTTTAGGAGGTCCTGTTCAAACCCCTGCATTGTTGTTTCCTCCCCAATCAATATTCATTCCACTTGTTGGATCTTCTTCATATTTCAAGCCCACAAGTGAGGAGGAGTGTTATAGATGATATAAGATTAAATTTGCCTTCACCTATTAGCTTAAACTTTTGGGTCAATAGACGATTTAACATAACAAAATGAAAAGGGAATTATAAAGATGGACTTGTTTTACATTGATTTTAAAGGTTTGATTGTTCTTGGGAAAAAAATATGCATAGTTGGAAGAATATGTGAAACTAAGAAGCATGAGCGCTCGGGAATAGGGCAAAAGAATGACATGGATGTGGGCACGAGATACAAATAACTCTTTTTTATAGATATGTCATACTTCGTTATAGTTGCATTTACAAGATAATGCGAAAAGTGTGACATGTACAATACTCTAAGAACTGATCCATCAGTTCGTGCAAATACAACAAATACTTAATGTCGTTATTTTCGTTCCTTTTTCTTGTAGATGTTGGTAACAAATGCCGAACAAGCTATCTTGAGCAAGGACCTGGTAATTTCCCTTCAGTTTTATTGGGGCACTCATGTAGAAAATTATTTATTTATTTATTGCTTATACTATATTTGGTTGATATTTTGGGATTATTCTCTTTTCTTTTGAACCATCAGGACTAGTTAACCCTTCTCCTTGGTACTATTTATGGTCTTAATCAATAATCAGTATTTTGGAGACGTGTCTCGCAGGAGGGCCTTTTTGAGATGTTGGTCATGGCCCTCAACTTCTTTAATTATTTTTTTATGACTTTTTGTAAATATATTAAGAAATATGTTCCGAAAATTGTAATTTTATTTAATCCCTTGGAATGGTCGACGGGTGAGGTTAGGCAGGTGAGTGGAATTCGAAGTGAGTATGTATCCATCGTTCCTTCTTATCACATAAATGCAAAAACCTATACTTGACTTGATGTAACAATTGGCATCCACCATGTTATTTTAAATTGTTGCTTCATCCATGTTATTTATGCTGCAGGCAATCTTGCGATCTGACCTTCCAGTCTACATGGTACCATTTACCACCAGAGATCTTTTATTCAAGAAACCGGAGGTTTGTGCTATTATTAATAAGACATGGCACTCTTGATTCTATTTTCTATCCTTTATTCAAGAAACCGGAGTATTTCAAATGCTGGATGGTAATTATTCTTCTTGAGTTCCAATTTTTCTACCATTTTAAAATATAAATATCAACAATGGAAGAGTGTTTTGAACTTTTTTTTAAAGGAAATTAAAAAAAAACATCGAAGTTCAAATGTGTACGTTTACCCACCCTTTTGTCTACCTTTGTTCTATTCTCTTTTTGTGGTCATAAAAAAATGGTTATATGTTCTCTTCATAAAAGAATTTGAATATTCCCACTGAAATTCTTAGTTTTCGCGTTATTTGTACATTTTTCTTTTTGGCCTCAAATGTTAGAGGTTTGATAGAATATTGGATTATATAGAATACTTTAGAAGCAATACAACTCTAAATTGTGACTAAAATTTTTGGTTGTATAGGATAATGGGGAGAAATTCACAAGCCTCTTAACTGCGATTGGTGCATATGCAGAAGGGTTCTCAGCTGATCCAATAATCAGGAGAGTACTCTACTTATGGAAGAAGCTAGAAAAAAATTAGTTATTGGTGTCTATATGTTCAATCTGTGTGCACACTTTGTTTCATGTAAATTTGGTTTTGTTGTATAGTTGGAGATATTAGAGAGCTTAGAAATCACATTATTCTTCTTTATGAATACCCCTTTCTCCTTGTTTTTCATAGCCTTTTAGCAAAGAAAATAGGAAACGCAAGAAGTTTCAGCTTAACATGTTATGGAGTATCCCTTGCTATGAAGGACTGAACTGGCTTCTCCCGTTGATTGATTATTTGAACATTCCAACCATATGATTGGTTCTTTAGAAAATGGAGGTATTTTGTTATTAGGAGTATTTTCATATAATTAAATTTACTGTGATTCATTTGCTTTAAAGATTGATGATTTGACATCTACACTATTGAAAGATGGTTCTAACGTGGATGAGGCACATTTCATAGTTGTGCAAAAGCTTATACTGTTGA

mRNA sequence

ATGTCTTCTATCGACTTGATGGTGCTTGATGTTCTTATATCGCTAAATGCAGGAGTCGATGGAATTGGAAATGTCAATGCTGTGCAACTTATCACTAGATTCGGCACGTTAGAAAATTTGTTGCAACATGTTGATCAAGTGGAAGATGAACGTATTAAGAAGATGTTGGTAACAAATGCCGAACAAGCTATCTTGAGCAAGGACCTGGTAATTTCCCTTCAGTTTTATTGGGGCACTCATGCAATCTTGCGATCTGACCTTCCAGTCTACATGGTACCATTTACCACCAGAGATCTTTTATTCAAGAAACCGGAGGATAATGGGGAGAAATTCACAAGCCTCTTAACTGCGATTGGTGCATATGCAGAAGGGTTCTCAGCTGATCCAATAATCAGGAGAGTACTCTACTTATGGAAGAAGCTAGAAAAAAATTAGTTATTGGTGTCTATATGTTCAATCTGTGTGCACACTTTGTTTCATGTAAATTTGGTTTTGTTGTATAGTTGGAGATATTAGAGAGCTTAGAAATCACATTATTCTTCTTTATGAATACCCCTTTCTCCTTGTTTTTCATAGCCTTTTAGCAAAGAAAATAGGAAACGCAAGAAGTTTCAGCTTAACATGTTATGGAGTATCCCTTGCTATGAAGGACTGAACTGGCTTCTCCCGTTGATTGATTATTTGAACATTCCAACCATATGATTGGTTCTTTAGAAAATGGAGGTATTTTGTTATTAGGAGTATTTTCATATAATTAAATTTACTGTGATTCATTTGCTTTAAAGATTGATGATTTGACATCTACACTATTGAAAGATGGTTCTAACGTGGATGAGGCACATTTCATAGTTGTGCAAAAGCTTATACTGTTGA

Coding sequence (CDS)

ATGTCTTCTATCGACTTGATGGTGCTTGATGTTCTTATATCGCTAAATGCAGGAGTCGATGGAATTGGAAATGTCAATGCTGTGCAACTTATCACTAGATTCGGCACGTTAGAAAATTTGTTGCAACATGTTGATCAAGTGGAAGATGAACGTATTAAGAAGATGTTGGTAACAAATGCCGAACAAGCTATCTTGAGCAAGGACCTGGTAATTTCCCTTCAGTTTTATTGGGGCACTCATGCAATCTTGCGATCTGACCTTCCAGTCTACATGGTACCATTTACCACCAGAGATCTTTTATTCAAGAAACCGGAGGATAATGGGGAGAAATTCACAAGCCTCTTAACTGCGATTGGTGCATATGCAGAAGGGTTCTCAGCTGATCCAATAATCAGGAGAGTACTCTACTTATGGAAGAAGCTAGAAAAAAATTAG

Protein sequence

MSSIDLMVLDVLISLNAGVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYWGTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYLWKKLEKN
Homology
[Close]
BLAST of Lsi01G020320 vs. ExPASy Swiss-Prot
Match: Q04957 (DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.0e-06
Identity = 36/102 (35.29%), Postives = 57/102 (55.88%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GV GIG   AV+L+ +FGT+EN+L  +D+++ E++K+ L  + E A+LSK L        
Sbjct: 192 GVPGIGEKTAVKLLRQFGTVENVLASIDEIKGEKLKETLRQHREMALLSKKL-------- 251

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIG 120
              A +R D PV +    + D +  + ED  EK  +L   +G
Sbjct: 252 ---AAIRRDAPVEL----SLDDIAYQGEDR-EKVVALFKELG 277

[Close]
BLAST of Lsi01G020320 vs. ExPASy Swiss-Prot
Match: P52026 (DNA polymerase I OS=Geobacillus stearothermophilus OX=1422 GN=polA PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 6.6e-06
Identity = 34/102 (33.33%), Postives = 57/102 (55.88%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GV GIG   AV+L+ +FGT+EN+L  +D+++ E++K+ L    + A+LSK L        
Sbjct: 192 GVPGIGEKTAVKLLKQFGTVENVLASIDEIKGEKLKENLRQYRDLALLSKQL-------- 251

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIG 120
              A +  D PV +   T  D+++K   ++ EK  +L   +G
Sbjct: 252 ---AAICRDAPVEL---TLDDIVYK--GEDREKVVALFQELG 277

[Close]
BLAST of Lsi01G020320 vs. ExPASy Swiss-Prot
Match: O34996 (DNA polymerase I OS=Bacillus subtilis (strain 168) OX=224308 GN=polA PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.0e-04
Identity = 19/52 (36.54%), Postives = 37/52 (71.15%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDL 70
           GV G+G   A++L+ +F ++E LL+ +D+V  +++K+ L    +QA++SK+L
Sbjct: 194 GVPGVGEKTAIKLLKQFDSVEKLLESIDEVSGKKLKEKLEEFKDQALMSKEL 245

[Close]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match: A0A6J1D847 (uncharacterized protein LOC111017796 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.7e-53
Identity = 112/132 (84.85%), Postives = 118/132 (89.39%), Query Frame = 0 

Query: 13  ISLNAGVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVIS 72
           + L+ GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L   
Sbjct: 319 LKLSRGVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL--- 378

Query: 73  LQFYWGTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIR 132
                   AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIR
Sbjct: 379 --------AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIR 438

Query: 133 RVLYLWKKLEKN 145
           RVLYLWKKLEKN
Sbjct: 439 RVLYLWKKLEKN 439

[Close]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match: A0A6J1D5H7 (DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 287 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 346

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 347 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 402

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 407 WKKLEKN 402

[Close]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match: A0A6J1D7Q6 (DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 318 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 377

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 378 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 433

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 438 WKKLEKN 433

[Close]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match: A0A6J1D5H5 (DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 336 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 395

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 396 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 451

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 456 WKKLEKN 451

[Close]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match: A0A1S3BMJ7 (DNA-directed DNA polymerase OS=Cucumis melo OX=3656 GN=LOC103491482 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.8e-52
Identity = 112/127 (88.19%), Postives = 113/127 (88.98%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDL        
Sbjct: 345 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDL-------- 404

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A LRSDLP YMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAE FSADPIIRRVLYL
Sbjct: 405 ---ATLRSDLPFYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAERFSADPIIRRVLYL 460

Query: 138 WKKLEKN 145
           WKKL+KN
Sbjct: 465 WKKLDKN 460

[Close]
BLAST of Lsi01G020320 vs. NCBI nr
Match: XP_022149356.1 (uncharacterized protein LOC111017796 isoform X2 [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 2.0e-52
Identity = 112/132 (84.85%), Postives = 118/132 (89.39%), Query Frame = 0 

Query: 13  ISLNAGVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVIS 72
           + L+ GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L   
Sbjct: 319 LKLSRGVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL--- 378

Query: 73  LQFYWGTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIR 132
                   AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIR
Sbjct: 379 --------AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIR 438

Query: 133 RVLYLWKKLEKN 145
           RVLYLWKKLEKN
Sbjct: 439 RVLYLWKKLEKN 439

[Close]
BLAST of Lsi01G020320 vs. NCBI nr
Match: XP_022149360.1 (uncharacterized protein LOC111017796 isoform X5 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 7.6e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 287 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 346

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 347 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 402

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 407 WKKLEKN 402

[Close]
BLAST of Lsi01G020320 vs. NCBI nr
Match: XP_022149355.1 (uncharacterized protein LOC111017796 isoform X1 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 7.6e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 336 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 395

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 396 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 451

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 456 WKKLEKN 451

[Close]
BLAST of Lsi01G020320 vs. NCBI nr
Match: XP_022149357.1 (uncharacterized protein LOC111017796 isoform X3 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 7.6e-52
Identity = 111/127 (87.40%), Postives = 115/127 (90.55%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERI+KMLVTNA QAILSK+L        
Sbjct: 318 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIRKMLVTNAGQAILSKNL-------- 377

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              AILRSDLP+YMVPFTTRDLLFKKPEDNGEKFTSL+TAIGAYAEGFSADPIIRRVLYL
Sbjct: 378 ---AILRSDLPLYMVPFTTRDLLFKKPEDNGEKFTSLMTAIGAYAEGFSADPIIRRVLYL 433

Query: 138 WKKLEKN 145
           WKKLEKN
Sbjct: 438 WKKLEKN 433

[Close]
BLAST of Lsi01G020320 vs. NCBI nr
Match: XP_008449676.1 (PREDICTED: DNA polymerase I [Cucumis melo])

HSP 1 Score: 213.8 bits (543), Expect = 1.0e-51
Identity = 112/127 (88.19%), Postives = 113/127 (88.98%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDL        
Sbjct: 345 GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDL-------- 404

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A LRSDLP YMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAE FSADPIIRRVLYL
Sbjct: 405 ---ATLRSDLPFYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAERFSADPIIRRVLYL 460

Query: 138 WKKLEKN 145
           WKKL+KN
Sbjct: 465 WKKLDKN 460

[Close]
BLAST of Lsi01G020320 vs. TAIR 10
Match: AT3G52050.2 (5'-3' exonuclease family protein )

HSP 1 Score: 159.1 bits (401), Expect = 2.7e-39
Identity = 81/125 (64.80%), Postives = 99/125 (79.20%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNV+AV+LI+RFGTLENLLQ VD++++ +IK+ L+ +A+QAILSK L        
Sbjct: 309 GVDGIGNVHAVELISRFGTLENLLQSVDEIKEGKIKESLIASADQAILSKKL-------- 368

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A+LRSDLP Y+VPF T+DL FKKPEDNGEK +SLL AI  YAEGFSADP+IRR   L
Sbjct: 369 ---ALLRSDLPDYIVPFDTKDLTFKKPEDNGEKLSSLLIAIADYAEGFSADPVIRRAFRL 422

Query: 138 WKKLE 143
           W+KLE
Sbjct: 429 WEKLE 422

[Close]
BLAST of Lsi01G020320 vs. TAIR 10
Match: AT3G52050.1 (5'-3' exonuclease family protein )

HSP 1 Score: 159.1 bits (401), Expect = 2.7e-39
Identity = 81/125 (64.80%), Postives = 99/125 (79.20%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNV+AV+LI+RFGTLENLLQ VD++++ +IK+ L+ +A+QAILSK L        
Sbjct: 309 GVDGIGNVHAVELISRFGTLENLLQSVDEIKEGKIKESLIASADQAILSKKL-------- 368

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A+LRSDLP Y+VPF T+DL FKKPEDNGEK +SLL AI  YAEGFSADP+IRR   L
Sbjct: 369 ---ALLRSDLPDYIVPFDTKDLTFKKPEDNGEKLSSLLIAIADYAEGFSADPVIRRAFRL 422

Query: 138 WKKLE 143
           W+KLE
Sbjct: 429 WEKLE 422

[Close]
BLAST of Lsi01G020320 vs. TAIR 10
Match: AT3G52050.3 (5'-3' exonuclease family protein )

HSP 1 Score: 159.1 bits (401), Expect = 2.7e-39
Identity = 81/125 (64.80%), Postives = 99/125 (79.20%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNV+AV+LI+RFGTLENLLQ VD++++ +IK+ L+ +A+QAILSK L        
Sbjct: 332 GVDGIGNVHAVELISRFGTLENLLQSVDEIKEGKIKESLIASADQAILSKKL-------- 391

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A+LRSDLP Y+VPF T+DL FKKPEDNGEK +SLL AI  YAEGFSADP+IRR   L
Sbjct: 392 ---ALLRSDLPDYIVPFDTKDLTFKKPEDNGEKLSSLLIAIADYAEGFSADPVIRRAFRL 445

Query: 138 WKKLE 143
           W+KLE
Sbjct: 452 WEKLE 445

[Close]
BLAST of Lsi01G020320 vs. TAIR 10
Match: AT3G52050.4 (5'-3' exonuclease family protein )

HSP 1 Score: 159.1 bits (401), Expect = 2.7e-39
Identity = 81/125 (64.80%), Postives = 99/125 (79.20%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKMLVTNAEQAILSKDLVISLQFYW 77
           GVDGIGNV+AV+LI+RFGTLENLLQ VD++++ +IK+ L+ +A+QAILSK L        
Sbjct: 297 GVDGIGNVHAVELISRFGTLENLLQSVDEIKEGKIKESLIASADQAILSKKL-------- 356

Query: 78  GTHAILRSDLPVYMVPFTTRDLLFKKPEDNGEKFTSLLTAIGAYAEGFSADPIIRRVLYL 137
              A+LRSDLP Y+VPF T+DL FKKPEDNGEK +SLL AI  YAEGFSADP+IRR   L
Sbjct: 357 ---ALLRSDLPDYIVPFDTKDLTFKKPEDNGEKLSSLLIAIADYAEGFSADPVIRRAFRL 410

Query: 138 WKKLE 143
           W+KLE
Sbjct: 417 WEKLE 410

[Close]
BLAST of Lsi01G020320 vs. TAIR 10
Match: AT3G52050.5 (5'-3' exonuclease family protein )

HSP 1 Score: 57.8 bits (138), Expect = 8.5e-09
Identity = 26/38 (68.42%), Postives = 36/38 (94.74%), Query Frame = 0 

Query: 18  GVDGIGNVNAVQLITRFGTLENLLQHVDQVEDERIKKM 56
           GVDGIGNV+AV+LI+RFGTLENLLQ VD++++ +IK++
Sbjct: 314 GVDGIGNVHAVELISRFGTLENLLQSVDEIKEGKIKEV 351

The following BLAST results are available for this feature:
BLAST of Lsi01G020320 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q049571.0e-0635.29DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1[more]
P520266.6e-0633.33DNA polymerase I OS=Geobacillus stearothermophilus OX=1422 GN=polA PE=1 SV=2[more]
O349968.0e-0436.54DNA polymerase I OS=Bacillus subtilis (strain 168) OX=224308 GN=polA PE=3 SV=1[more]
BLAST of Lsi01G020320 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D8479.7e-5384.85uncharacterized protein LOC111017796 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D5H73.7e-5287.40DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 ... [more]
A0A6J1D7Q63.7e-5287.40DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 ... [more]
A0A6J1D5H53.7e-5287.40DNA-directed DNA polymerase OS=Momordica charantia OX=3673 GN=LOC111017796 PE=4 ... [more]
A0A1S3BMJ74.8e-5288.19DNA-directed DNA polymerase OS=Cucumis melo OX=3656 GN=LOC103491482 PE=4 SV=1[more]
BLAST of Lsi01G020320 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022149356.12.0e-5284.85uncharacterized protein LOC111017796 isoform X2 [Momordica charantia][more]
XP_022149360.17.6e-5287.40uncharacterized protein LOC111017796 isoform X5 [Momordica charantia][more]
XP_022149355.17.6e-5287.40uncharacterized protein LOC111017796 isoform X1 [Momordica charantia][more]
XP_022149357.17.6e-5287.40uncharacterized protein LOC111017796 isoform X3 [Momordica charantia][more]
XP_008449676.11.0e-5188.19PREDICTED: DNA polymerase I [Cucumis melo][more]
BLAST of Lsi01G020320 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G52050.22.7e-3964.805'-3' exonuclease family protein [more]
AT3G52050.12.7e-3964.805'-3' exonuclease family protein [more]
AT3G52050.32.7e-3964.805'-3' exonuclease family protein [more]
AT3G52050.42.7e-3964.805'-3' exonuclease family protein [more]
AT3G52050.58.5e-0968.425'-3' exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020045DNA polymerase I-like, H3TH domainPFAMPF013675_3_exonuccoord: 18..70
e-value: 1.2E-11
score: 45.1
NoneNo IPR availableGENE3D1.10.150.20coord: 16..71
e-value: 7.8E-12
score: 47.4
NoneNo IPR availablePANTHERPTHR10133:SF545'-3' EXONUCLEASE FAMILY PROTEINcoord: 17..143
IPR002298DNA polymerase APANTHERPTHR10133DNA POLYMERASE Icoord: 17..143
IPR0362795'-3' exonuclease, C-terminal domain superfamilySUPERFAMILY478075' to 3' exonuclease, C-terminal subdomaincoord: 17..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G020320.1Lsi01G020320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006261 DNA-dependent DNA replication
molecular_function GO:0003677 DNA binding
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity