Lsi01G020290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G020290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRNA exonuclease 4
Locationchr01: 27781797 .. 27789597 (-)
RNA-Seq ExpressionLsi01G020290
SyntenyLsi01G020290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATCCTTATGAATTGATCCCTCAAAAGTTTGGAACTAATTTGTTCAATTTGGACATAAATTCCTAGGAAGAATAAAAAGAAGTATATTTATGATGAGGTCAAATAAAAGAAGAAAAAAAAACGAAGAAGGAAACTGGAAAAGAGAAGAACCTCAGAGTTCATCTTCCTCCCTTTCTCACTCACAGCCGCTGTCACACTCTTCTGTCGGAGTTTCATCTCACTGGCGAGCCCCCGACGACGACGACGGCAAGTGTTCCTGTTCGCCAGCAACCTCAGGCCACGCGACTCACGCGTTGACGGCGTTACCCACACTGCAATCCCGTGCGTTTGAGTTGCGTTTCCTCCTCTGGCGAGACCCACGAGTGGTGGTGAATCCGTCTTTGTAGCTGCACGACAGCAGGCTCTAGTGAAAGTTACGTGCGACGTTGGTTCAGCGACAGCTGCACTACGGGCTTCCCGGCGGCCGCATAAGCAAACAGATCCGACACCTATGATTGTCGGCGTTTCGATAGTGACCCACCAGAGCCTTCGCAGCAAAGTTTCTCTCCTTTTCGACGAGTTTTGGCTTTGACCCACACTCCAATTATAGTTTTGGACTGGTTCGAAGAGATCGATTAAGGTTTATGGTTAATTTTGGGTAAAAATATCCCTCCTCAACATTCTCTCATGAACTGAGTTTTAGCCTCTAAGCATTGTCTTAATTCTTTAGACTTCTAGGAAGTTGATATACTGCAAGTTTAGTAGTTTCCACATCCTTTCAGAACTATAAATTTTGGCCTAATTATGTTTTGATTATTTTAATTTGACAAACGTTTGAAAGAATGTTTTGAATGTTTGAAAATATGTTTTGTATAAGTTGAAGTTGATCGAAATAGTTTTGTACGCTTTATTTGAAAGTATGTTTGCAGTTTGATAATTTGAAATAAAATGTTTTGATTGATTTTGAATTGGAAGTAGGTTTGTTTTAGTTTTGTCACGCATTAAATTATTGAAAATATAGAATGGTTTGTTTTGATGAATTCTTTTATTGAATTTGCTAATTTGAAACTGTTTCAAATGGTTATGTAAGATTCTTCAAAAAAAAAAAAAAGAAAAAAATCCTTATATAAGAATTTTGATCAATTTGAAAGGGTTATCTGGATATGCCAATTTTGTTTTGACATTGTATGTTTATTTTGTAGTAGCCAATTTTGGAATGGACAGTTTATGATATGGATGAAACTTATTTGTGAGAAGCCACTTTATAGTGGTTTTGAAAGCTCATTTGAATGAAAGATTTTTGTTTTGAAAGCATTGTTTGTAACAAAGTTCAATGAATCATATTTTTTGTTGATGAGTGTTTTGAAAGACAATGAATCTAAAAGCCTGATATGAGCATTGCTGACAGAGTACTTTTGAACTCATTTTTGCTTTTCCCAATATGTTTTCAGATGAAAAATTAAGTGATGGTTGATGGTTCTAAAAGAAGTGAAGTTTGACATTCCGAACAATGTTTTTTTTAATATCAATTTTTTTTTTTTTTTTAAAGAATTGATAGTTTATCTTAGAAATCATTTGTAATACATCGCTTCTGCTTGATAAAGTTTCTAATGGTTATGTTTTCTACACTATTGTTATGCTAGCTTTATTGAAAAATACAAGCTGTTGCACTAGATTCCAGAGGAGAGGAACATACACTAACAGTTTTGGCTCCGCATGGACTCCGTCTCCGATCATGACCCTCCCAAGAAACTAAGCCTAAGGTTGCATTCTTGCTCTTACACAGTTACTTTTAGTTTTAGGTTTCTTATTGTCAACATCCTTTCCTTGTTTTTGTTTATTGGGGTTTTATAGAGCGACAAGAAGTAAAAACAACATGTTTTTACCTGTTGTCCTTCCCTGTTTGTTGGTCTTTATTGTCCATTAATTTCTTTAGGAGTGTCATTCCTAGTGGAACTCAGGTGAATCACTGTTATTCATTTCGTGTTTTCTCCCACCCATGCTTGACACCATCCTCTTTGTATTTGATGAAGACGATCATTTTGTCAATGTTAGGTTCTTATATCCTGCACTCAGTAAAACCCAACAAGGAAAAGACAAACAGAGCAAGAACCTCTGTGATATGCACTATTCAAGCGAAAAGATTACCAAGTCTACAACAAGGTCTCCAAGTACAGCACAATGTCAAATAACAGTGACAATAGTAACCTAAATGGAAATGACAAGAAATGAAAATGGTAATAGCAAAACTAGCACATTCGAGAGAACTAGCTGTCGTCTCCTCCCTAAAAGCCACACTGAGCTTTTACCAAAAATGAATCTGCCTTCTCCAAATCCCTGCTTCCCTATTTGAGGTAATATTCTTGGTCCCCACTTCCCAACTCATTCTTCCTCCCTTCCTCCGTAACTACTTTGTCAGCTACTGTCTTCTGTGAAATTCCTCTTCTACCCCTCCGTTTGTAAGTATATATTATTGGGGGTTTAACAGTCAACCTGAGCAATAACTTTTGGCTTTCAAAGAGGTTCTCGATCTTGTGATTCATACCAATGCAATGATCATCGTGACTGTTGATTCTTCAATCGTAACATATTGAGGTGTGAATGGTTCTCTCTCCTCTCTCCTACCTCTTCCTATCCCCCACTCTCTTACCCTCGTCCTTCACCCATCACGCCGGAGCCACCAACCATGACCTTCACCTCTGGACACTCCAACTCCTCCAACCATGATCAAAACCCAGCCGCCCATCAAGATTACATAGAAAGGAAGGTCTTCTCAATCGACATTGACCCAAACTCTCGAAGCAAAAGAGTCAAAATAACATAGTAGAATGGTGCCACTCACTAAATTTGAAGAACAAATTTTTTGCTAACTCCCTTCATGCAGCCTAAAGGGAATGCCTTGGTATAACCTCTTTTCAATAACTTCTCAAAGACTAAGTGTAGCAATAGGCATAAAGAAAATTAAAGAAAGTGTATGGGAACTGAAAGAATAACACAATTGTTGGTGTAAATCTTCTCGCCACCTTTCCAGCGAAATGTTTTTGACAATTTCTATCATCTCTTGCTTGTCTCCTGCAAAGTGATAGGCGACTAGACATTTTCCTCCCGTGTACTGCCTGAGAACCTCATGATGATAAAAGGGGGAGATGATATATCTCTAGAAGGCTGAGTTATAAAATAGATTCTTGATTGACTATTATGTATAGAATCATAATGAGGTTGTACTGTTCTGTTATTTTAGCTGAATTGAATCTGAATTGCCTTCTTATTAAAGAAGAAGAAAGCTATCATGGAAACTCCCATCATGACCATGGAATGTGTAGGCTTGTCATCTTTTCATTTAACCCTCCGCTATGGTTTCTACTTCATATTGAGTACTTGATGGTGAACATGAGAATTATTGGGTACATTTGTTTACAGACATAAATGCTCTGCATGCTACAAGCAATATAAGAAGAAGGAGCACCTTATTGAGCATATGAGAGTTTCATATCACTCTGTTCATCAGCCTAGATGTGGAGTCTGCCTAAAGCACTGCAAATCATTTGAATCATTGAGGGAACATCTAAAGGGTGAGATAGTTTTGTGTATTTTTTATGGTAATATACCATAACACCATCTTATTGTATCCAGTCTTTTAGGTCCACTTTCAAAATCAAATTGCTCAAAAATATTCTCGGAGCAAGGTTGTGGGCTTTGTTTGAGAGTACTTGATGGTCCCGGATCTCTCAGTGAGCACCAAGACATTTGCTGCCTAACAGCACCTGTTCACCAAGTAAGTCGCTCTCTATGCATCATATATCATATATGATATTAATTAATAAGGTTACTGGTAGTTCTCATTAGAAAAAATAAGGTTACTGGTAGTTTATATTTTTGGATATATCAAGTAATTGAGCCATTTATTATGATGTCATACATGCATTTCAAAATTTTATTGGGGCTCTGATGTAGGGAACAAGTCTATTGCCAACTGATTTATCAGATTGTTACGATGAAGACCGTTCTGATCGAGGCCTTGGAGCAATAGCTATAGATTGTGTAATGGCTGGCGGTGGAAGTGATGGTGCACTGGACATTTGTGTTTGGATCTGCCTTGTTGATGAAGATGAGAAATTGATTTTCAGTACTTTTGTACAACCACAAATACCAATCACGAATTACAGGTACTTGTCTGTGGCATTATCTTGCTCTTTAGAGAAGGTTTAAGCTTTTAGAAACCAGCCTGTAGATGCTCATTTCCCAGTCATGTTGTAGGCGTGAAGTAACCGGGCTAAAGGAAGAACATATGAGGTATGCCATGCCACTGAAAAAAGTCCAAGAAAAGGTATTAAGAATCTTGTTAAATGGAGAATCCATTGGGAGATTGAGATTGAATGGTGGTAAAGCTAGGCTTCTTGTCGGCCATGACTTGGAGCATGATTTAGATTGCTTGAGAATCAATTATCCCGATCATATGTTAAGGTGATTTTCACTAACTTGGGTTCTAAGTATGTGTGGTCCTGTGGCTTAGTTCACCTTTATTGAAAACGAAATGCATTTGAACTCTGAAGTGATCTGGTGAAATGCAATGCACAAAAAACGATCAAATACTACTTTTCAACCCTGCACGTTATTTTCTCTGGAAATAATTCCTACCGCTGAGGAAAACAATGTATTTTTTTCCTCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

GAAATCCTTATGAATTGATCCCTCAAAAGTTTGGAACTAATTTGTTCAATTTGGACATAAATTCCTAGGAAGAATAAAAAGAAGTATATTTATGATGAGGTCAAATAAAAGAAGAAAAAAAAACGAAGAAGGAAACTGGAAAAGAGAAGAACCTCAGAGTTCATCTTCCTCCCTTTCTCACTCACAGCCGCTGTCACACTCTTCTGTCGGAGTTTCATCTCACTGGCGAGCCCCCGACGACGACGACGGCAAGTGTTCCTGTTCGCCAGCAACCTCAGGCCACGCGACTCACGCGTTGACGGCGTTACCCACACTGCAATCCCGTGCGTTTGAGTTGCGTTTCCTCCTCTGGCGAGACCCACGAGTGGTGGTGAATCCGTCTTTGTAGCTGCACGACAGCAGGCTCTAGTGAAAGTTACGTGCGACGTTGGTTCAGCGACAGCTGCACTACGGGCTTCCCGGCGGCCGCATAAGCAAACAGATCCGACACCTATGATTGTCGGCGTTTCGATAGTGACCCACCAGAGCCTTCGCAGCAAAGTTTCTCTCCTTTTCGACGAGTTTTGGCTTTGACCCACACTCCAATTATAGTTTTGGACTGGTTCGAAGAGATCGATTAAGGTTTATGGTTAATTTTGGCTTTATTGAAAAATACAAGCTGTTGCACTAGATTCCAGAGGAGAGGAACATACACTAACAGTTTTGGCTCCGCATGGACTCCGTCTCCGATCATGACCCTCCCAAGAAACTAAGCCTAAGACATAAATGCTCTGCATGCTACAAGCAATATAAGAAGAAGGAGCACCTTATTGAGCATATGAGAGTTTCATATCACTCTGTTCATCAGCCTAGATGTGGAGTCTGCCTAAAGCACTGCAAATCATTTGAATCATTGAGGGAACATCTAAAGGGTCCACTTTCAAAATCAAATTGCTCAAAAATATTCTCGGAGCAAGGTTGTGGGCTTTGTTTGAGAGTACTTGATGGTCCCGGATCTCTCAGTGAGCACCAAGACATTTGCTGCCTAACAGCACCTGTTCACCAAGGAACAAGTCTATTGCCAACTGATTTATCAGATTGTTACGATGAAGACCGTTCTGATCGAGGCCTTGGAGCAATAGCTATAGATTGTGTAATGGCTGGCGGTGGAAGTGATGGTGCACTGGACATTTGTGTTTGGATCTGCCTTGTTGATGAAGATGAGAAATTGATTTTCAGTACTTTTGTACAACCACAAATACCAATCACGAATTACAGGTACTTGCGTGAAGTAACCGGGCTAAAGGAAGAACATATGAGGTATGCCATGCCACTGAAAAAAGTCCAAGAAAAGGTATTAAGAATCTTGTTAAATGGAGAATCCATTGGGAGATTGAGATTGAATGGTGGTAAAGCTAGGCTTCTTGTCGGCCATGACTTGGAGCATGATTTAGATTGCTTGAGAATCAATTATCCCGATCATATGTTAAGGTGATTTTCACTAACTTGGGTTCTAAGTATGTGTGGTCCTGTGGCTTAGTTCACCTTTATTGAAAACGAAATGCATTTGAACTCTGAAGTGATCTGGTGAAATGCAATGCACAAAAAACGATCAAATACTACTTTTCAACCCTGCACGTTATTTTCTCTGGAAATAATTCCTACCGCTGAGGAAAACAATGGACACCGCTAGATATCATCCATTGATGAAAACAAATTTGGTTAGCCATTCTCTCAAGTACCTTACTCGAGCATATTTGGGGTATGATATTCGTCAGGGTGGGCACAACCCCTACGAGAATTGTGTATCAGTTATGCGATTATACAAGAGAATGCGTAGTCTTGATCACCGTGCACAAGTTTTGACACCATTGATTGCTCCTCCCGCTATCCAGTATGATGCTCCCAATCTTGATTCCCATAGTGTAAAAGATCTTGAGAAGATGACTCCAGATGAACTTTATGAGATGTCAAGATCAAACTTTAAATGTTGGTGTCTTGACTCAAGGCGAGTAATGCAGACTTAGGTGGTGTCGGCAATTTGTATAGACAGTACTCATTTTGCTGAGGAAGAGGTGAGGTTGTTGGAAGACACGCTTCAGTGGGCCTTATTTGTTGAGAAAGGGGGGAGTGAGAGGGGAAAGGTTGACCTGTTCTATTGTATCTATCTATCCTTTCAAGACTTAAAACGACTAAAGTCGTATTGGATGTTTTTCTTGCCATATGAATATTCATATGATGGTGTTTATTTCCTCGACTACCTTTTGTTTATTTACCTGGTCCCTTTTTTTTTTCCAAGCCATCATTAATACCCTTGAATCTTTCAAAACTCTTCTGTCTTTGTCTTTTGCTTCTTCTTCGATTACGGTTTGGGAGATTTGGGTTAGTTGAGAATAATTTTTTAAAACAGAATCCTCCAATACTTTATTTCTTTTGATTTTCGGGGATTTGAGTTGGTTTAGAAGGTTGGGC

Coding sequence (CDS)

ATGGACTCCGTCTCCGATCATGACCCTCCCAAGAAACTAAGCCTAAGACATAAATGCTCTGCATGCTACAAGCAATATAAGAAGAAGGAGCACCTTATTGAGCATATGAGAGTTTCATATCACTCTGTTCATCAGCCTAGATGTGGAGTCTGCCTAAAGCACTGCAAATCATTTGAATCATTGAGGGAACATCTAAAGGGTCCACTTTCAAAATCAAATTGCTCAAAAATATTCTCGGAGCAAGGTTGTGGGCTTTGTTTGAGAGTACTTGATGGTCCCGGATCTCTCAGTGAGCACCAAGACATTTGCTGCCTAACAGCACCTGTTCACCAAGGAACAAGTCTATTGCCAACTGATTTATCAGATTGTTACGATGAAGACCGTTCTGATCGAGGCCTTGGAGCAATAGCTATAGATTGTGTAATGGCTGGCGGTGGAAGTGATGGTGCACTGGACATTTGTGTTTGGATCTGCCTTGTTGATGAAGATGAGAAATTGATTTTCAGTACTTTTGTACAACCACAAATACCAATCACGAATTACAGGTACTTGCGTGAAGTAACCGGGCTAAAGGAAGAACATATGAGGTATGCCATGCCACTGAAAAAAGTCCAAGAAAAGGTATTAAGAATCTTGTTAAATGGAGAATCCATTGGGAGATTGAGATTGAATGGTGGTAAAGCTAGGCTTCTTGTCGGCCATGACTTGGAGCATGATTTAGATTGCTTGAGAATCAATTATCCCGATCATATGTTAAGGTGA

Protein sequence

MDSVSDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREHLKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHMLR
Homology
BLAST of Lsi01G020290 vs. ExPASy Swiss-Prot
Match: Q4IEV5 (RNA exonuclease 4 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=REX4 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.7e-10
Identity = 38/118 (32.20%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 136 IAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLKEEHM 195
           IAIDC M G G  G       + +VD     I+ ++V+P+  +TN+R    V+G+ ++ M
Sbjct: 134 IAIDCEMVGVGPGGHESALARVSIVDFHGVQIYDSYVKPKEKVTNWR--TAVSGISQKSM 193

Query: 196 RYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHMLR 254
           R+A   ++VQ ++ ++L              + R+LVGHDL+HDL+ L +++P   +R
Sbjct: 194 RFARDFEEVQAEIDKLL--------------RGRILVGHDLKHDLEALILSHPGKDIR 235

BLAST of Lsi01G020290 vs. ExPASy Swiss-Prot
Match: Q7S9B7 (RNA exonuclease 4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=rex-4 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 41/136 (30.15%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 118 TDLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIP 177
           TDL+      +S+     ++IDC M G G  GA  +     +VD     I+ ++V+P   
Sbjct: 199 TDLALILQATKSNTLGKYLSIDCEMVGTGPSGATSVLARCSIVDFHGHQIYDSYVRPTAF 258

Query: 178 ITNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLE 237
           +T++R    V+G+ + HM  A   + VQ  V  +L              K R+LVGHD++
Sbjct: 259 VTDWR--THVSGISKRHMASARSFESVQATVAALL--------------KGRILVGHDVK 318

Query: 238 HDLDCLRINYPDHMLR 254
           HDL+ L   +P   +R
Sbjct: 319 HDLEVLGFEHPHRDIR 318

BLAST of Lsi01G020290 vs. ExPASy Swiss-Prot
Match: Q08237 (RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=REX4 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 38/118 (32.20%), Postives = 62/118 (52.54%), Query Frame = 0

Query: 136 IAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLKEEHM 195
           IA+DC   G G +G       I +V+    ++   FV+P+  +  +R    V+G+K EHM
Sbjct: 122 IAMDCEFVGVGPEGKESALARISIVNYFGHVVLDEFVKPREKVVEWR--TWVSGIKPEHM 181

Query: 196 RYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHMLR 254
           + A+  K+ Q+K   IL              + R+LVGH L+HDL+ L +++P  +LR
Sbjct: 182 KNAITFKEAQKKTADIL--------------EGRILVGHALKHDLEALMLSHPKSLLR 223

BLAST of Lsi01G020290 vs. ExPASy Swiss-Prot
Match: Q6CMT3 (RNA exonuclease 4 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=REX4 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.5e-09
Identity = 35/118 (29.66%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 136 IAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLKEEHM 195
           +++DC   G G DG       + +V+    ++   FV+P+ P+T++R    V+G+K  HM
Sbjct: 120 VSMDCEFVGVGPDGKDSALARVSIVNYYGNVVLDLFVRPKEPVTDWR--TWVSGIKPHHM 179

Query: 196 RYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHMLR 254
             A+  +  Q++V  +L              K R+LVGH + HDL  L +++P  M+R
Sbjct: 180 ANAVTQEDCQKQVSNVL--------------KGRILVGHSVHHDLTALMLSHPRRMIR 221

BLAST of Lsi01G020290 vs. ExPASy Swiss-Prot
Match: B2GUW6 (Apoptosis-enhancing nuclease OS=Rattus norvegicus OX=10116 GN=Aen PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.2e-08
Identity = 31/118 (26.27%), Postives = 60/118 (50.85%), Query Frame = 0

Query: 136 IAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLKEEHM 195
           +AIDC M G G  G +       +V     +++  +++P++PI +YR     +G+  +HM
Sbjct: 106 VAIDCEMVGTGPQGRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYR--TRWSGITRQHM 165

Query: 196 RYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHMLR 254
             A+P +  Q+++L++L              K +++VGH L +D   L+  +P   +R
Sbjct: 166 HKAIPFQVAQKEILKLL--------------KGKVVVGHALHNDFQALKYVHPGSQIR 207

BLAST of Lsi01G020290 vs. ExPASy TrEMBL
Match: A0A1S4E1J6 (uncharacterized protein LOC103495972 OS=Cucumis melo OX=3656 GN=LOC103495972 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.2e-126
Identity = 220/249 (88.35%), Postives = 235/249 (94.38%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           S HDP K  SLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLRE+
Sbjct: 3   SHHDPLKTPSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREY 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L GPLSKS+CSKIFSEQGCGLCLRVLD P +LSEH +ICC+TAPVHQGTSLLPTDLSDCY
Sbjct: 63  LMGPLSKSSCSKIFSEQGCGLCLRVLDSPKTLSEHLNICCITAPVHQGTSLLPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           +EDRSDRGLGAIA+DCVMAGGGSDGALDICVWICLVDE EKLIF+TFVQPQIPITNYR+ 
Sbjct: 123 EEDRSDRGLGAIAMDCVMAGGGSDGALDICVWICLVDEHEKLIFNTFVQPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGLKEEH+RYAMPL+ VQEKVL++LLNGESIGRLR NGGKA+LLVGHDLEHDLD LR
Sbjct: 183 -EVTGLKEEHLRYAMPLRNVQEKVLKLLLNGESIGRLRSNGGKAKLLVGHDLEHDLDYLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 MNYPDHMLR 249

BLAST of Lsi01G020290 vs. ExPASy TrEMBL
Match: A0A6J1DNJ6 (RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111022742 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 4.9e-124
Identity = 216/249 (86.75%), Postives = 233/249 (93.57%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           SD DP    + RHKCSACYKQYKKKEHLIEHM+VSYHS+HQPRCGVC KHCKSFESLREH
Sbjct: 3   SDRDPLNPPTTRHKCSACYKQYKKKEHLIEHMKVSYHSIHQPRCGVCGKHCKSFESLREH 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L+GPLSKSNCSKIF EQGCGLCLRVLDG  SL+EH+DICCLTAPVHQGTSLLPTDLSDCY
Sbjct: 63  LQGPLSKSNCSKIFIEQGCGLCLRVLDGRRSLNEHRDICCLTAPVHQGTSLLPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           DEDRSDR LGAIA+DCVMAGGGSDG LD+CV +CLVDE+EKLIF+TFV+PQIPITNYR+ 
Sbjct: 123 DEDRSDRVLGAIAMDCVMAGGGSDGTLDLCVGVCLVDEEEKLIFNTFVRPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGL EEHMRYAMPLK+VQEKVLRIL+NGESIGRLRLNGG+ARLLVGHDLEHDLDCLR
Sbjct: 183 -EVTGLTEEHMRYAMPLKEVQEKVLRILINGESIGRLRLNGGRARLLVGHDLEHDLDCLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 MNYPDHMLR 249

BLAST of Lsi01G020290 vs. ExPASy TrEMBL
Match: A0A6J1K8M7 (RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111491129 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.8e-119
Identity = 213/242 (88.02%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 12  KLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREHLKGPLSK 71
           K  LRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVC KHCKSFESLREHL GPLSK
Sbjct: 8   KECLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCQKHCKSFESLREHLNGPLSK 67

Query: 72  SNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCYDEDRSDR 131
           SNCSKIF EQGCGLCLRVLD   SLS HQDIC LTAPV QGTSLLPTDLSDCYDEDRSDR
Sbjct: 68  SNCSKIFLEQGCGLCLRVLDDRTSLSGHQDICRLTAPVRQGTSLLPTDLSDCYDEDRSDR 127

Query: 132 GLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLK 191
           GLGAIA+DCVMAGGGSDG LDIC+ ICLVDEDEKLIF+TFVQPQIPI NYR+  EVTGL 
Sbjct: 128 GLGAIAMDCVMAGGGSDGTLDICIGICLVDEDEKLIFNTFVQPQIPIANYRH--EVTGLT 187

Query: 192 EEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHM 251
           EE +RYAMPLK+VQEKVL+ILLNGE+I RLR+NGGKARLLVGHDLEHDLDCLR+NYPDHM
Sbjct: 188 EERVRYAMPLKEVQEKVLKILLNGETIERLRVNGGKARLLVGHDLEHDLDCLRMNYPDHM 247

Query: 252 LR 254
           LR
Sbjct: 248 LR 247

BLAST of Lsi01G020290 vs. ExPASy TrEMBL
Match: A0A6J1EYI8 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111437384 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.7e-119
Identity = 213/242 (88.02%), Postives = 222/242 (91.74%), Query Frame = 0

Query: 12  KLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREHLKGPLSK 71
           K  LRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVC KHCKSFESLREHL GPLSK
Sbjct: 8   KECLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCQKHCKSFESLREHLNGPLSK 67

Query: 72  SNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCYDEDRSDR 131
           SNCSKIF EQGCGLCLRVLD   SL  HQDIC LTAPV QGTSLLPTDLSDCYDEDRSDR
Sbjct: 68  SNCSKIFLEQGCGLCLRVLDDRTSLRGHQDICRLTAPVRQGTSLLPTDLSDCYDEDRSDR 127

Query: 132 GLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLK 191
           GLGAIA+DCVMAGGGSDG LDIC+ ICLVDEDEKLIF+TFVQP IPITNYR+  EVTGL 
Sbjct: 128 GLGAIAMDCVMAGGGSDGTLDICIGICLVDEDEKLIFNTFVQPHIPITNYRH--EVTGLT 187

Query: 192 EEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHM 251
           EE +RYAMPLK+VQEKVL+ILLNGE+I RLRLNGGKARLLVGHDLEHDLDCLR+NYPDHM
Sbjct: 188 EERVRYAMPLKEVQEKVLKILLNGETIERLRLNGGKARLLVGHDLEHDLDCLRMNYPDHM 247

Query: 252 LR 254
           LR
Sbjct: 248 LR 247

BLAST of Lsi01G020290 vs. ExPASy TrEMBL
Match: A0A6J1ETD0 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111437384 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.7e-119
Identity = 213/242 (88.02%), Postives = 222/242 (91.74%), Query Frame = 0

Query: 12  KLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREHLKGPLSK 71
           K  LRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVC KHCKSFESLREHL GPLSK
Sbjct: 16  KECLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCQKHCKSFESLREHLNGPLSK 75

Query: 72  SNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCYDEDRSDR 131
           SNCSKIF EQGCGLCLRVLD   SL  HQDIC LTAPV QGTSLLPTDLSDCYDEDRSDR
Sbjct: 76  SNCSKIFLEQGCGLCLRVLDDRTSLRGHQDICRLTAPVRQGTSLLPTDLSDCYDEDRSDR 135

Query: 132 GLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREVTGLK 191
           GLGAIA+DCVMAGGGSDG LDIC+ ICLVDEDEKLIF+TFVQP IPITNYR+  EVTGL 
Sbjct: 136 GLGAIAMDCVMAGGGSDGTLDICIGICLVDEDEKLIFNTFVQPHIPITNYRH--EVTGLT 195

Query: 192 EEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINYPDHM 251
           EE +RYAMPLK+VQEKVL+ILLNGE+I RLRLNGGKARLLVGHDLEHDLDCLR+NYPDHM
Sbjct: 196 EERVRYAMPLKEVQEKVLKILLNGETIERLRLNGGKARLLVGHDLEHDLDCLRMNYPDHM 255

Query: 252 LR 254
           LR
Sbjct: 256 LR 255

BLAST of Lsi01G020290 vs. NCBI nr
Match: XP_011653750.1 (apoptosis-enhancing nuclease isoform X1 [Cucumis sativus] >XP_011653752.1 apoptosis-enhancing nuclease isoform X1 [Cucumis sativus] >XP_031739807.1 apoptosis-enhancing nuclease isoform X1 [Cucumis sativus] >XP_031739808.1 apoptosis-enhancing nuclease isoform X1 [Cucumis sativus])

HSP 1 Score: 484.6 bits (1246), Expect = 5.3e-133
Identity = 231/249 (92.77%), Postives = 240/249 (96.39%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           SDHDP K  SLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH
Sbjct: 3   SDHDPLKTPSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L GPLSKSNCSKIFSEQGCGLCLRVLDGP SLS+HQDICC+TAPVHQGTSL PTDLSDCY
Sbjct: 63  LMGPLSKSNCSKIFSEQGCGLCLRVLDGPESLSDHQDICCITAPVHQGTSLPPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           +EDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIF+TFVQPQIPITNYR+ 
Sbjct: 123 EEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFNTFVQPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGLKEEHMRYAMPLK VQEKVL++LLNGESIGRLRLNGGKA+LLVGHDLEHDLDCLR
Sbjct: 183 -EVTGLKEEHMRYAMPLKNVQEKVLKLLLNGESIGRLRLNGGKAKLLVGHDLEHDLDCLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 LNYPDHMLR 249

BLAST of Lsi01G020290 vs. NCBI nr
Match: XP_038877619.1 (uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_038877620.1 uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_038877621.1 uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_038877622.1 uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_038877623.1 uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_038877625.1 uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida])

HSP 1 Score: 471.5 bits (1212), Expect = 4.7e-129
Identity = 227/249 (91.16%), Postives = 236/249 (94.78%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           SDHDP K  SLRHKCSACYKQYKKK+HLIEHMRVSYHSVHQPRCGVCLKH KSFESLREH
Sbjct: 3   SDHDPLKTPSLRHKCSACYKQYKKKDHLIEHMRVSYHSVHQPRCGVCLKHFKSFESLREH 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           LKGPL KSNCSKIFSE+GCGLCLRVLDGPGSLSEHQ+ICCLTAPVHQG SLLPTDLSDCY
Sbjct: 63  LKGPLPKSNCSKIFSERGCGLCLRVLDGPGSLSEHQNICCLTAPVHQGNSLLPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
            EDRSDRG GAIA+DCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYR+ 
Sbjct: 123 GEDRSDRGHGAIAMDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGLKEEHMR+A+PLK VQEKVL+ILLNGESI RLR NGGKARLLVGHDLEHDLDCLR
Sbjct: 183 -EVTGLKEEHMRHAIPLKNVQEKVLKILLNGESIERLRFNGGKARLLVGHDLEHDLDCLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 MNYPDHMLR 249

BLAST of Lsi01G020290 vs. NCBI nr
Match: XP_016901860.1 (PREDICTED: uncharacterized protein LOC103495972 [Cucumis melo])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 220/249 (88.35%), Postives = 235/249 (94.38%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           S HDP K  SLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLRE+
Sbjct: 3   SHHDPLKTPSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREY 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L GPLSKS+CSKIFSEQGCGLCLRVLD P +LSEH +ICC+TAPVHQGTSLLPTDLSDCY
Sbjct: 63  LMGPLSKSSCSKIFSEQGCGLCLRVLDSPKTLSEHLNICCITAPVHQGTSLLPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           +EDRSDRGLGAIA+DCVMAGGGSDGALDICVWICLVDE EKLIF+TFVQPQIPITNYR+ 
Sbjct: 123 EEDRSDRGLGAIAMDCVMAGGGSDGALDICVWICLVDEHEKLIFNTFVQPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGLKEEH+RYAMPL+ VQEKVL++LLNGESIGRLR NGGKA+LLVGHDLEHDLD LR
Sbjct: 183 -EVTGLKEEHLRYAMPLRNVQEKVLKLLLNGESIGRLRSNGGKAKLLVGHDLEHDLDYLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 MNYPDHMLR 249

BLAST of Lsi01G020290 vs. NCBI nr
Match: XP_022155663.1 (apoptosis-enhancing nuclease isoform X1 [Momordica charantia])

HSP 1 Score: 453.8 bits (1166), Expect = 1.0e-123
Identity = 216/249 (86.75%), Postives = 233/249 (93.57%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           SD DP    + RHKCSACYKQYKKKEHLIEHM+VSYHS+HQPRCGVC KHCKSFESLREH
Sbjct: 3   SDRDPLNPPTTRHKCSACYKQYKKKEHLIEHMKVSYHSIHQPRCGVCGKHCKSFESLREH 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L+GPLSKSNCSKIF EQGCGLCLRVLDG  SL+EH+DICCLTAPVHQGTSLLPTDLSDCY
Sbjct: 63  LQGPLSKSNCSKIFIEQGCGLCLRVLDGRRSLNEHRDICCLTAPVHQGTSLLPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           DEDRSDR LGAIA+DCVMAGGGSDG LD+CV +CLVDE+EKLIF+TFV+PQIPITNYR+ 
Sbjct: 123 DEDRSDRVLGAIAMDCVMAGGGSDGTLDLCVGVCLVDEEEKLIFNTFVRPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGL EEHMRYAMPLK+VQEKVLRIL+NGESIGRLRLNGG+ARLLVGHDLEHDLDCLR
Sbjct: 183 -EVTGLTEEHMRYAMPLKEVQEKVLRILINGESIGRLRLNGGRARLLVGHDLEHDLDCLR 242

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 MNYPDHMLR 249

BLAST of Lsi01G020290 vs. NCBI nr
Match: XP_004142658.1 (apoptosis-enhancing nuclease isoform X2 [Cucumis sativus] >KGN54753.2 hypothetical protein Csa_012744 [Cucumis sativus])

HSP 1 Score: 448.7 bits (1153), Expect = 3.2e-122
Identity = 218/249 (87.55%), Postives = 227/249 (91.16%), Query Frame = 0

Query: 5   SDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 64
           SDHDP K  SLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH
Sbjct: 3   SDHDPLKTPSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREH 62

Query: 65  LKGPLSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPTDLSDCY 124
           L              EQGCGLCLRVLDGP SLS+HQDICC+TAPVHQGTSL PTDLSDCY
Sbjct: 63  L-------------MEQGCGLCLRVLDGPESLSDHQDICCITAPVHQGTSLPPTDLSDCY 122

Query: 125 DEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYL 184
           +EDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIF+TFVQPQIPITNYR+ 
Sbjct: 123 EEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFNTFVQPQIPITNYRH- 182

Query: 185 REVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLR 244
            EVTGLKEEHMRYAMPLK VQEKVL++LLNGESIGRLRLNGGKA+LLVGHDLEHDLDCLR
Sbjct: 183 -EVTGLKEEHMRYAMPLKNVQEKVLKLLLNGESIGRLRLNGGKAKLLVGHDLEHDLDCLR 236

Query: 245 INYPDHMLR 254
           +NYPDHMLR
Sbjct: 243 LNYPDHMLR 236

BLAST of Lsi01G020290 vs. TAIR 10
Match: AT2G48100.1 (Exonuclease family protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.9e-70
Identity = 128/255 (50.20%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 1   MDSVSDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFES 60
           MDS  +    +K+S+RH+C ACYK + ++EHL+EHM++SYHS+HQPRCGVCLKHCKSFES
Sbjct: 1   MDSQLNPSKRRKISVRHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFES 60

Query: 61  LREHLKGP--LSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPT 120
           +REHL  P  LSK NC  IF+++GC LCL++ +   +L+EH++ C L+ P   GTS    
Sbjct: 61  VREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRN 120

Query: 121 DLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPI 180
             S       +   L A+A+DC M GGG+DG +D C  +CLVD+DE +IFST VQP +P+
Sbjct: 121 PSSSL-----AGSRLKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPV 180

Query: 181 TNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEH 240
           T+YR+  E+TGL +E ++  MPL+ V+E+V   L  G++ G  RL      LLVGHDL H
Sbjct: 181 TDYRH--EITGLTKEDLKDGMPLEHVRERVFSFLCGGQNDGAGRL------LLVGHDLRH 240

Query: 241 DLDCLRINYPDHMLR 254
           D+ CL++ YP H+LR
Sbjct: 241 DMSCLKLEYPSHLLR 242

BLAST of Lsi01G020290 vs. TAIR 10
Match: AT2G48100.2 (Exonuclease family protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.9e-70
Identity = 128/255 (50.20%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 1   MDSVSDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFES 60
           MDS  +    +K+S+RH+C ACYK + ++EHL+EHM++SYHS+HQPRCGVCLKHCKSFES
Sbjct: 1   MDSQLNPSKRRKISVRHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFES 60

Query: 61  LREHLKGP--LSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPT 120
           +REHL  P  LSK NC  IF+++GC LCL++ +   +L+EH++ C L+ P   GTS    
Sbjct: 61  VREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRN 120

Query: 121 DLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPI 180
             S       +   L A+A+DC M GGG+DG +D C  +CLVD+DE +IFST VQP +P+
Sbjct: 121 PSSSL-----AGSRLKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPV 180

Query: 181 TNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEH 240
           T+YR+  E+TGL +E ++  MPL+ V+E+V   L  G++ G  RL      LLVGHDL H
Sbjct: 181 TDYRH--EITGLTKEDLKDGMPLEHVRERVFSFLCGGQNDGAGRL------LLVGHDLRH 240

Query: 241 DLDCLRINYPDHMLR 254
           D+ CL++ YP H+LR
Sbjct: 241 DMSCLKLEYPSHLLR 242

BLAST of Lsi01G020290 vs. TAIR 10
Match: AT2G48100.3 (Exonuclease family protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.9e-70
Identity = 128/255 (50.20%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 1   MDSVSDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFES 60
           MDS  +    +K+S+RH+C ACYK + ++EHL+EHM++SYHS+HQPRCGVCLKHCKSFES
Sbjct: 1   MDSQLNPSKRRKISVRHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFES 60

Query: 61  LREHLKGP--LSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPT 120
           +REHL  P  LSK NC  IF+++GC LCL++ +   +L+EH++ C L+ P   GTS    
Sbjct: 61  VREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRN 120

Query: 121 DLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPI 180
             S       +   L A+A+DC M GGG+DG +D C  +CLVD+DE +IFST VQP +P+
Sbjct: 121 PSSSL-----AGSRLKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPV 180

Query: 181 TNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEH 240
           T+YR+  E+TGL +E ++  MPL+ V+E+V   L  G++ G  RL      LLVGHDL H
Sbjct: 181 TDYRH--EITGLTKEDLKDGMPLEHVRERVFSFLCGGQNDGAGRL------LLVGHDLRH 240

Query: 241 DLDCLRINYPDHMLR 254
           D+ CL++ YP H+LR
Sbjct: 241 DMSCLKLEYPSHLLR 242

BLAST of Lsi01G020290 vs. TAIR 10
Match: AT2G48100.4 (Exonuclease family protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.9e-70
Identity = 128/255 (50.20%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 1   MDSVSDHDPPKKLSLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFES 60
           MDS  +    +K+S+RH+C ACYK + ++EHL+EHM++SYHS+HQPRCGVCLKHCKSFES
Sbjct: 1   MDSQLNPSKRRKISVRHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFES 60

Query: 61  LREHLKGP--LSKSNCSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQGTSLLPT 120
           +REHL  P  LSK NC  IF+++GC LCL++ +   +L+EH++ C L+ P   GTS    
Sbjct: 61  VREHLNVPDHLSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRN 120

Query: 121 DLSDCYDEDRSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPI 180
             S       +   L A+A+DC M GGG+DG +D C  +CLVD+DE +IFST VQP +P+
Sbjct: 121 PSSSL-----AGSRLKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPV 180

Query: 181 TNYRYLREVTGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEH 240
           T+YR+  E+TGL +E ++  MPL+ V+E+V   L  G++ G  RL      LLVGHDL H
Sbjct: 181 TDYRH--EITGLTKEDLKDGMPLEHVRERVFSFLCGGQNDGAGRL------LLVGHDLRH 240

Query: 241 DLDCLRINYPDHMLR 254
           D+ CL++ YP H+LR
Sbjct: 241 DMSCLKLEYPSHLLR 242

BLAST of Lsi01G020290 vs. TAIR 10
Match: AT3G27970.1 (Exonuclease family protein )

HSP 1 Score: 257.7 bits (657), Expect = 9.8e-69
Identity = 124/246 (50.41%), Postives = 170/246 (69.11%), Query Frame = 0

Query: 14  SLRHKCSACYKQYKKKEHLIEHMRVSYHSVHQPRCGVCLKHCKSFESLREHLKGPLSKSN 73
           +LR+KC+ACY+Q+ K EHL+EHM++SYHS H+P CGVC KHC+SFESLREHL GPL K  
Sbjct: 12  TLRNKCAACYRQFNKLEHLVEHMKISYHSGHEPTCGVCKKHCRSFESLREHLIGPLPKQE 71

Query: 74  CSKIFSEQGCGLCLRVLDGPGSLSEHQDICCLTAPVHQG--TSLLPTDLSD----CYDED 133
           C  IFS +GC  C+ +L+ P S   HQ+ C  ++ V+ G  T +    L D     Y   
Sbjct: 72  CKNIFSLRGCRFCMTILESPNSRRIHQERCQFSS-VNSGLTTRMAALGLRDKAMIDYTSS 131

Query: 134 RSDRGLGAIAIDCVMAGGGSDGALDICVWICLVDEDEKLIFSTFVQPQIPITNYRYLREV 193
           RS R    +A+ C M GGGSDG+LD+C  +C+ DE + +IF T+V+P + +T+YRY  E 
Sbjct: 132 RSPR---VVALSCKMVGGGSDGSLDLCARVCITDESDNVIFHTYVKPSMAVTSYRY--ET 191

Query: 194 TGLKEEHMRYAMPLKKVQEKVLRILLNGESIGRLRLNGGKARLLVGHDLEHDLDCLRINY 253
           TG++ E++R AMPLK+VQ K+   L NGE + ++R  GGKAR+LVGH L+HDLD L++ Y
Sbjct: 192 TGIRPENLRDAMPLKQVQRKIQEFLCNGEPMWKIRPRGGKARILVGHGLDHDLDRLQLEY 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4IEV52.7e-1032.20RNA exonuclease 4 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9... [more]
Q7S9B72.2e-0930.15RNA exonuclease 4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708... [more]
Q082372.2e-0932.20RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55... [more]
Q6CMT36.5e-0929.66RNA exonuclease 4 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 707... [more]
B2GUW63.2e-0826.27Apoptosis-enhancing nuclease OS=Rattus norvegicus OX=10116 GN=Aen PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4E1J65.2e-12688.35uncharacterized protein LOC103495972 OS=Cucumis melo OX=3656 GN=LOC103495972 PE=... [more]
A0A6J1DNJ64.9e-12486.75RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111022742 PE=3 SV=1[more]
A0A6J1K8M72.8e-11988.02RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111491129 PE=3 SV=1[more]
A0A6J1EYI84.7e-11988.02RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111437384 PE=3 SV=1[more]
A0A6J1ETD04.7e-11988.02RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111437384 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011653750.15.3e-13392.77apoptosis-enhancing nuclease isoform X1 [Cucumis sativus] >XP_011653752.1 apopto... [more]
XP_038877619.14.7e-12991.16uncharacterized protein LOC120069877 isoform X1 [Benincasa hispida] >XP_03887762... [more]
XP_016901860.11.1e-12588.35PREDICTED: uncharacterized protein LOC103495972 [Cucumis melo][more]
XP_022155663.11.0e-12386.75apoptosis-enhancing nuclease isoform X1 [Momordica charantia][more]
XP_004142658.13.2e-12287.55apoptosis-enhancing nuclease isoform X2 [Cucumis sativus] >KGN54753.2 hypothetic... [more]
Match NameE-valueIdentityDescription
AT2G48100.18.9e-7050.20Exonuclease family protein [more]
AT2G48100.28.9e-7050.20Exonuclease family protein [more]
AT2G48100.38.9e-7050.20Exonuclease family protein [more]
AT2G48100.48.9e-7050.20Exonuclease family protein [more]
AT3G27970.19.8e-6950.41Exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 134..253
e-value: 6.4E-21
score: 76.6
NoneNo IPR availablePANTHERPTHR12801:SF123EXONUCLEASE FAMILY PROTEINcoord: 14..253
NoneNo IPR availablePANTHERPTHR12801RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATEDcoord: 14..253
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 19..41
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 17..46
score: 11.593128
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 136..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G020290.1Lsi01G020290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding