Lsi01G020260 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G020260 vs. ExPASy Swiss-Prot
Match: P0DH90 (Protein FRIGIDA OS=Arabidopsis thaliana OX=3702 GN=FRI PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 4.8e-51
Identity = 127/300 (42.33%), Postives = 180/300 (60.00%), Query Frame = 0

Query: 52  LGSESEPQEASFQFLKISYVDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHIS 111
           +G   +P     QFLK   +DEL + S A+  F+ + ++LQ H+  I NAID++      
Sbjct: 47  IGQSKQP-----QFLK--SIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 112 LSSSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSEL 171
           L S+  ++A     RN       LS  PR+N+   ++       +E V   PE+S+  E 
Sbjct: 107 LESNGVVLA----ARNNNFHQPMLS-PPRNNVSVETTVTVSQPSQEIV---PETSNKPEG 166

Query: 172 EHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYS 231
             +CE MCS+GLRKYI +++SD A L  EIP ALK A  PAK V DCIG+FYLQG +A++
Sbjct: 167 GRMCELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFT 226

Query: 232 KDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGS 291
           K+SPM  ARQ S+LILE FL+      +   + +IE  +K EA+ AA+AWRKRL+ E G 
Sbjct: 227 KESPMSSARQVSLLILESFLL---MPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGL 286

Query: 292 CQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIVD 351
             A  +DARGLLL +A FG+P+ F + DL DL+R S S  I+  L+ S +L+  +  IV+
Sbjct: 287 AAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQFLVPMVSGIVE 322

BLAST of Lsi01G020260 vs. ExPASy Swiss-Prot
Match: Q67Z93 (Inactive protein FRIGIDA OS=Arabidopsis thaliana OX=3702 GN=FRI PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.3e-50
Identity = 125/290 (43.10%), Postives = 175/290 (60.34%), Query Frame = 0

Query: 52  LGSESEPQEASFQFLKISYVDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHIS 111
           +G   +P     QFLK   +DEL + S A+  F+ + ++LQ H+  I NAID++      
Sbjct: 47  IGQSKQP-----QFLK--SIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 112 LSSSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSEL 171
           L S+  ++A     RN       LS  PR+N+   ++       +E V   PE+S+  E 
Sbjct: 107 LESNGVVLA----ARNNNFHQPMLS-PPRNNVSVETTVTVSQPSQEIV---PETSNKPEG 166

Query: 172 EHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYS 231
           E +CE MCS+GLRKYI +++SD A L  EIP ALK A  PAK V DCIG+FYLQG +A++
Sbjct: 167 ERICELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFT 226

Query: 232 KDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGS 291
           K+SPM  ARQ S+LILE FL+      +   + +IE  +K EA+ AA+AWRKRL+ E G 
Sbjct: 227 KESPMSSARQVSLLILESFLL---MPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGL 286

Query: 292 CQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHY 342
             A  +DARGLLL +A FG+P+ F + DL DL+R S S  I+  L+ S +
Sbjct: 287 AAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQF 312

BLAST of Lsi01G020260 vs. ExPASy Swiss-Prot
Match: Q67ZB3 (FRIGIDA-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=FRL3 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.5e-12
Identity = 88/358 (24.58%), Postives = 158/358 (44.13%), Query Frame = 0

Query: 29  EEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISYVDE-----LGSLSSAIH- 88
           E+ RS  S  D    K   QL++  +E E Q A    LK   ++E       SL    H 
Sbjct: 2   EDTRSVASLMDSTSSKIQ-QLQKAFAELESQRAVTLNLKWKELEEHFHGLERSLKRRFHE 61

Query: 89  ------AFQCRLNELQDHLGFIHNAIDARSKQHISL------SSSSSIVATAESGRN-PV 148
                  ++ +  + Q+ L     A++A+ K  +        ++  +I +  +   N PV
Sbjct: 62  LEDQEKEYETKTRKAQELLEKKKAAVEAKEKAALERLQKKRDAAMFTINSALDKYNNAPV 121

Query: 149 SSSTHLSRSPRSNLPESSSDFG----KIDGEEGVNKQPESS---------SLSELEHLCE 208
           S  +   R P++ + +SS+ F       D  +G+ +  + S         +  +L  LC 
Sbjct: 122 SKPSVGERWPQNAVEDSSNVFAADSITDDNPDGIVQDVQISPVMGNYEVKAYPQLLKLCG 181

Query: 209 TMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAY--SKDS 268
            M S GL K++  +  +LASL+ EIP+A + A NPA LV D +  FY   +      KD+
Sbjct: 182 DMDSTGLHKFVSDNRKNLASLKEEIPMAFRAAANPASLVLDSLEGFYPMEAPTADGKKDA 241

Query: 269 PMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVN-ESGSCQ 328
            ++  R+  I+++E   I  +   +N     +  ++K  A   A  W   L + +  +C 
Sbjct: 242 NLLGMRRTCIMLMECLSILLSGLDRNCLAVVLSQNVKHRAKTIAEGWNPLLESLDMDACN 301

Query: 329 ASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIVD 352
            + ++A   L  LA+F I   F  D+L  L+   + +  +  L  S  L  ++P +++
Sbjct: 302 GNSLEAHAFLQLLATFAIVADFKEDELLKLIPMVSRRRQAAELCRSLGLAEKMPGVIE 358

BLAST of Lsi01G020260 vs. ExPASy Swiss-Prot
Match: A0SWL0 (FRIGIDA-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=FRL2 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.9e-07
Identity = 62/246 (25.20%), Postives = 108/246 (43.90%), Query Frame = 0

Query: 79  SAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSSSSSIVATAESGRNPVSSSTHLSRS 138
           +A  +    +N++ +    +  A D   + H SL S S  ++ +E   +   SS   S  
Sbjct: 2   TAAESIAASINQIDEKKQKLKKAFD-DLQAHRSLLSPSFNLSWSEIDSH--FSSLQSSLF 61

Query: 139 PRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLASLR 198
            R     +SS+ G I+    V  +       EL   CE    +GL  Y++ +     S+ 
Sbjct: 62  NRLQSAVTSSNSGNIETPTAVTTE-TPVLWPELRKFCEKNDGKGLGNYMIENSRKRLSIN 121

Query: 199 HEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPARQASILILELFLISGAAET 258
            E+P A++C+ NPA LV D I   Y   S + S  +  I  ++  +L+LE  LI   A  
Sbjct: 122 EELPNAIRCSENPAALVLDAIEGSYHCSSPSSSSSARAIDVKRIFVLLLEA-LIEINANL 181

Query: 259 KNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLASFGIPTVFTND 318
            ND        L+  A   A  W+  + N+         +A G L  +A+F + ++F+ +
Sbjct: 182 TND--------LRERARTIAYDWKPNIGNKPS-------EALGFLHLVAAFELGSLFSTE 227

Query: 319 DLRDLL 325
           ++ D +
Sbjct: 242 EICDYI 227

BLAST of Lsi01G020260 vs. ExPASy Swiss-Prot
Match: Q9C6S2 (Inactive FRIGIDA-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=FRL2 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.9e-07
Identity = 62/246 (25.20%), Postives = 108/246 (43.90%), Query Frame = 0

Query: 79  SAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSSSSSIVATAESGRNPVSSSTHLSRS 138
           +A  +    +N++ +    +  A D   + H SL S S  ++ +E   +   SS   S  
Sbjct: 2   TAAESIAASINQIDEKKQKLKKAFD-DLQAHRSLLSPSFNLSWSEIDSH--FSSLQSSLF 61

Query: 139 PRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLASLR 198
            R     +SS+ G I+    V  +       EL   CE    +GL  Y++ +     S+ 
Sbjct: 62  NRLQSAVTSSNSGNIETPTAVTTE-TPVLWPELRKFCEKNDGKGLGNYMIENSRKRLSIN 121

Query: 199 HEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPARQASILILELFLISGAAET 258
            E+P A++C+ NPA LV D I   Y   S + S  +  I  ++  +L+LE  LI   A  
Sbjct: 122 EELPNAIRCSENPAPLVLDAIEGSYHCSSPSSSSSARAIDVKRIFVLLLEA-LIEINANL 181

Query: 259 KNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLASFGIPTVFTND 318
            ND        L+  A   A  W+  + N+         +A G L  +A+F + ++F+ +
Sbjct: 182 TND--------LRERARTIAYDWKPNIGNKPS-------EALGFLHLVAAFELGSLFSTE 227

Query: 319 DLRDLL 325
           ++ D +
Sbjct: 242 EICDYI 227

BLAST of Lsi01G020260 vs. ExPASy TrEMBL
Match: A0A6J1EYU0 (FRIGIDA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439873 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 1.0e-141
Identity = 276/362 (76.24%), Postives = 303/362 (83.70%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESEPQE  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRPLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSSIVAT + GRNPVSSS+HL RSPR+NLPE+SSDFGK DG+E ++KQPESS L
Sbjct: 121 IALPYNSSSIVATLKRGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSPL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSDL SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+VNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. ExPASy TrEMBL
Match: A0A6J1HVU4 (FRIGIDA-like protein OS=Cucurbita maxima OX=3661 GN=LOC111467142 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 4.0e-141
Identity = 274/362 (75.69%), Postives = 303/362 (83.70%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESEPQ+  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQKTQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSS+VAT +SGRNPVSSS+HL RSPR+NLPE+S DFGK DG+E ++KQPESSSL
Sbjct: 121 IALPYNSSSVVATLKSGRNPVSSSSHLLRSPRNNLPETSRDFGKNDGKEAMDKQPESSSL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSDL SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+ NE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIFNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. ExPASy TrEMBL
Match: A0A5D3DV89 (FRIGIDA-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G001270 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.4e-140
Identity = 275/351 (78.35%), Postives = 296/351 (84.33%), Query Frame = 0

Query: 16  MAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISYVDELG 75
           MAA VDSF    K+EE S+      RL + PCQLRELGSES PQ+  F+ LK SY DELG
Sbjct: 1   MAATVDSF----KDEEHSEAV---SRLVEGPCQLRELGSESNPQQPPFKLLKNSYFDELG 60

Query: 76  SLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSS----------------SSSIV 135
           SLS+AIHAFQCR NELQDHL FIHNAIDARSKQH+S S+                SS++V
Sbjct: 61  SLSTAIHAFQCRFNELQDHLCFIHNAIDARSKQHMSSSNSLLDNHSQLLSSLSFKSSTLV 120

Query: 136 ATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMC 195
            TAESGRNPV SSTH SRSPRSNLPE+ +  GK+DG+EGV+KQ ESSSLSELEHLC+TMC
Sbjct: 121 PTAESGRNPVYSSTHPSRSPRSNLPETRTYSGKVDGKEGVDKQTESSSLSELEHLCQTMC 180

Query: 196 SRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPA 255
           SRGLRKYIVSHLSDLA+LRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAY+KDSPMIPA
Sbjct: 181 SRGLRKYIVSHLSDLANLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYTKDSPMIPA 240

Query: 256 RQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDA 315
           RQASILILELFLISGAAETKNDKRTEIE SLKVEADLAAIAWRKRL+ ESGSCQASDIDA
Sbjct: 241 RQASILILELFLISGAAETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQASDIDA 300

Query: 316 RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIV 351
           RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKG+S  L  SH L TRIP I+
Sbjct: 301 RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDII 344

BLAST of Lsi01G020260 vs. ExPASy TrEMBL
Match: A0A1S3BM50 (FRIGIDA-like protein OS=Cucumis melo OX=3656 GN=LOC103491359 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.4e-140
Identity = 275/351 (78.35%), Postives = 296/351 (84.33%), Query Frame = 0

Query: 16  MAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISYVDELG 75
           MAA VDSF    K+EE S+      RL + PCQLRELGSES PQ+  F+ LK SY DELG
Sbjct: 1   MAATVDSF----KDEEHSEAV---SRLVEGPCQLRELGSESNPQQPPFKLLKNSYFDELG 60

Query: 76  SLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSS----------------SSSIV 135
           SLS+AIHAFQCR NELQDHL FIHNAIDARSKQH+S S+                SS++V
Sbjct: 61  SLSTAIHAFQCRFNELQDHLCFIHNAIDARSKQHMSSSNSLLDNHSQLLSSLSFKSSTLV 120

Query: 136 ATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMC 195
            TAESGRNPV SSTH SRSPRSNLPE+ +  GK+DG+EGV+KQ ESSSLSELEHLC+TMC
Sbjct: 121 PTAESGRNPVYSSTHPSRSPRSNLPETRTYSGKVDGKEGVDKQTESSSLSELEHLCQTMC 180

Query: 196 SRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPA 255
           SRGLRKYIVSHLSDLA+LRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAY+KDSPMIPA
Sbjct: 181 SRGLRKYIVSHLSDLANLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYTKDSPMIPA 240

Query: 256 RQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDA 315
           RQASILILELFLISGAAETKNDKRTEIE SLKVEADLAAIAWRKRL+ ESGSCQASDIDA
Sbjct: 241 RQASILILELFLISGAAETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQASDIDA 300

Query: 316 RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIV 351
           RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKG+S  L  SH L TRIP I+
Sbjct: 301 RGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDII 344

BLAST of Lsi01G020260 vs. ExPASy TrEMBL
Match: A0A0A0KYX9 (FRIGIDA-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G437530 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.7e-107
Identity = 228/335 (68.06%), Postives = 242/335 (72.24%), Query Frame = 0

Query: 16  MAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISYVDELG 75
           MAA VDS     KEEE S+   TD RLG+ PCQL  L                       
Sbjct: 4   MAATVDS----SKEEEHSE---TDSRLGEGPCQLSSL----------------------- 63

Query: 76  SLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSSSSSIVATAESGRNPVSSSTHL 135
                                                S+SS +V TAESGRNPV SSTHL
Sbjct: 64  ------------------------------------FSNSSPLVPTAESGRNPVYSSTHL 123

Query: 136 SRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLA 195
           SRS RSNLPE+ +DFGK+DG+EGV+KQPESSSLS+LEHLC TMCSRGLRKYIVSHLSDLA
Sbjct: 124 SRSSRSNLPETRTDFGKVDGKEGVDKQPESSSLSQLEHLCRTMCSRGLRKYIVSHLSDLA 183

Query: 196 SLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPARQASILILELFLISGA 255
            L HEIPLALK APNPAKLVFDCIGRFYLQGSKAY+K SPMIPARQASILILELFLISGA
Sbjct: 184 RLHHEIPLALKWAPNPAKLVFDCIGRFYLQGSKAYTKVSPMIPARQASILILELFLISGA 243

Query: 256 AETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLASFGIPTVF 315
           AETKNDKRTEIE SLKVEADLAAIAWRKRL+ ESGSCQASDIDARGLLLFLASFGIPTVF
Sbjct: 244 AETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQASDIDARGLLLFLASFGIPTVF 272

Query: 316 TNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIV 351
           TNDDLRDLLRSSNSKG+S  L  SH L TRIP I+
Sbjct: 304 TNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDII 272

BLAST of Lsi01G020260 vs. NCBI nr
Match: XP_038890349.1 (protein FRIGIDA [Benincasa hispida])

HSP 1 Score: 530.0 bits (1364), Expect = 1.7e-146
Identity = 285/343 (83.09%), Postives = 303/343 (88.34%), Query Frame = 0

Query: 8   MSLMANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLK 67
           MSLMAN+ +A+AVDSFVEA+KEEE    SKTD RLG+ PCQ     S+ +PQE  FQFLK
Sbjct: 1   MSLMANLPLASAVDSFVEAVKEEE----SKTDARLGEGPCQ-----SKPDPQEPPFQFLK 60

Query: 68  ISYVDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSSSSSIVATAESGRN 127
            SYVDELGSLS+AI AF CR NELQDHLGFIHNAIDAR KQH  +  SS IVATAESGRN
Sbjct: 61  YSYVDELGSLSTAIRAFHCRFNELQDHLGFIHNAIDARFKQH--MFDSSIIVATAESGRN 120

Query: 128 PVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYI 187
           PVSSS     SPRSNLPE+ SDFGKIDG EGV+KQPESSSLSEL+HLCETMCSRGLRKYI
Sbjct: 121 PVSSS-----SPRSNLPETRSDFGKIDGREGVDKQPESSSLSELQHLCETMCSRGLRKYI 180

Query: 188 VSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPARQASILIL 247
           VSHLSDLA LRHEIPLAL+CAPNPAKLVFDCIGRFYLQGSKAY+KDSPMIPARQASILIL
Sbjct: 181 VSHLSDLARLRHEIPLALQCAPNPAKLVFDCIGRFYLQGSKAYTKDSPMIPARQASILIL 240

Query: 248 ELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLA 307
           ELFLIS AAETK DKRTEIEPSLKVEADLAAIAWRKRLV ESGSCQASDIDARGLLLFLA
Sbjct: 241 ELFLISSAAETKTDKRTEIEPSLKVEADLAAIAWRKRLVTESGSCQASDIDARGLLLFLA 300

Query: 308 SFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIV 351
           SFGIPTVFTNDDLRDLLRSSNSKGIS  LR SH+L++RIP I+
Sbjct: 301 SFGIPTVFTNDDLRDLLRSSNSKGISNALRRSHFLISRIPDII 327

BLAST of Lsi01G020260 vs. NCBI nr
Match: KAG6603163.1 (Protein FRIGIDA, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.2 bits (1323), Expect = 9.7e-142
Identity = 277/362 (76.52%), Postives = 304/362 (83.98%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESEPQE  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSSIVAT +SGRNPVSSS+HL RSPR+NLPE+SSDFGK DG+E ++KQPESSSL
Sbjct: 121 IALPYNSSSIVATLKSGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSD  SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDPDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+VNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. NCBI nr
Match: XP_022933109.1 (protein FRIGIDA [Cucurbita moschata])

HSP 1 Score: 513.1 bits (1320), Expect = 2.2e-141
Identity = 276/362 (76.24%), Postives = 303/362 (83.70%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESEPQE  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRPLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSSIVAT + GRNPVSSS+HL RSPR+NLPE+SSDFGK DG+E ++KQPESS L
Sbjct: 121 IALPYNSSSIVATLKRGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSPL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSDL SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+VNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. NCBI nr
Match: XP_022967688.1 (protein FRIGIDA [Cucurbita maxima])

HSP 1 Score: 511.1 bits (1315), Expect = 8.2e-141
Identity = 274/362 (75.69%), Postives = 303/362 (83.70%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESEPQ+  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQKTQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSS+VAT +SGRNPVSSS+HL RSPR+NLPE+S DFGK DG+E ++KQPESSSL
Sbjct: 121 IALPYNSSSVVATLKSGRNPVSSSSHLLRSPRNNLPETSRDFGKNDGKEAMDKQPESSSL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSDL SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+ NE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIFNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. NCBI nr
Match: KAG7033482.1 (Protein FRIGIDA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 511.1 bits (1315), Expect = 8.2e-141
Identity = 276/362 (76.24%), Postives = 303/362 (83.70%), Query Frame = 0

Query: 11  MANISMAAAVDSFVEAMKEEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISY 70
           M N +MAAAVDS +EA+K EE S+ SKTD +L K  CQL ELGSESE QE  F FLK SY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESESQETQFPFLKFSY 60

Query: 71  VDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLS----------------- 130
           +DELGSLSSAIHAFQCRL+ELQDHLG IHNAIDARSK+ IS S                 
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 131 -----SSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSL 190
                +SSSIVAT +SGRNPVSSS+HL RSPR+NLPE+SSDFGK DG+E ++KQPESSSL
Sbjct: 121 IALPYNSSSIVATLKSGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180

Query: 191 SELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSK 250
           SELEHLCETMCSRGLRKYIVSHLSD  SLRHEIPLALK APNPA+LVFDCIGRFYLQG K
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDPDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 251 AYSKDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNE 310
           AYSKDSPM+ ARQAS+LILELFLISG+AET+ND+R +IEPSLKVEA  AAIAWRKR+VNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 311 SGSCQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPA 351
           SGSC+ASDIDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKGIS  LR SH L TRIP 
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

BLAST of Lsi01G020260 vs. TAIR 10
Match: AT4G00650.1 (FRIGIDA-like protein )

HSP 1 Score: 199.9 bits (507), Expect = 3.8e-51
Identity = 125/290 (43.10%), Postives = 175/290 (60.34%), Query Frame = 0

Query: 52  LGSESEPQEASFQFLKISYVDELGSLSSAIHAFQCRLNELQDHLGFIHNAIDARSKQHIS 111
           +G   +P     QFLK   +DEL + S A+  F+ + ++LQ H+  I NAID++      
Sbjct: 47  IGQSKQP-----QFLK--SIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 112 LSSSSSIVATAESGRNPVSSSTHLSRSPRSNLPESSSDFGKIDGEEGVNKQPESSSLSEL 171
           L S+  ++A     RN       LS  PR+N+   ++       +E V   PE+S+  E 
Sbjct: 107 LESNGVVLA----ARNNNFHQPMLS-PPRNNVSVETTVTVSQPSQEIV---PETSNKPEG 166

Query: 172 EHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYS 231
           E +CE MCS+GLRKYI +++SD A L  EIP ALK A  PAK V DCIG+FYLQG +A++
Sbjct: 167 ERICELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFT 226

Query: 232 KDSPMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVNESGS 291
           K+SPM  ARQ S+LILE FL+      +   + +IE  +K EA+ AA+AWRKRL+ E G 
Sbjct: 227 KESPMSSARQVSLLILESFLL---MPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGL 286

Query: 292 CQASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHY 342
             A  +DARGLLL +A FG+P+ F + DL DL+R S S  I+  L+ S +
Sbjct: 287 AAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQF 312

BLAST of Lsi01G020260 vs. TAIR 10
Match: AT5G48385.1 (FRIGIDA-like protein )

HSP 1 Score: 75.5 bits (184), Expect = 1.1e-13
Identity = 88/358 (24.58%), Postives = 158/358 (44.13%), Query Frame = 0

Query: 29  EEERSQVSKTDERLGKEPCQLRELGSESEPQEASFQFLKISYVDE-----LGSLSSAIH- 88
           E+ RS  S  D    K   QL++  +E E Q A    LK   ++E       SL    H 
Sbjct: 2   EDTRSVASLMDSTSSKIQ-QLQKAFAELESQRAVTLNLKWKELEEHFHGLERSLKRRFHE 61

Query: 89  ------AFQCRLNELQDHLGFIHNAIDARSKQHISL------SSSSSIVATAESGRN-PV 148
                  ++ +  + Q+ L     A++A+ K  +        ++  +I +  +   N PV
Sbjct: 62  LEDQEKEYETKTRKAQELLEKKKAAVEAKEKAALERLQKKRDAAMFTINSALDKYNNAPV 121

Query: 149 SSSTHLSRSPRSNLPESSSDFG----KIDGEEGVNKQPESS---------SLSELEHLCE 208
           S  +   R P++ + +SS+ F       D  +G+ +  + S         +  +L  LC 
Sbjct: 122 SKPSVGERWPQNAVEDSSNVFAADSITDDNPDGIVQDVQISPVMGNYEVKAYPQLLKLCG 181

Query: 209 TMCSRGLRKYIVSHLSDLASLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAY--SKDS 268
            M S GL K++  +  +LASL+ EIP+A + A NPA LV D +  FY   +      KD+
Sbjct: 182 DMDSTGLHKFVSDNRKNLASLKEEIPMAFRAAANPASLVLDSLEGFYPMEAPTADGKKDA 241

Query: 269 PMIPARQASILILELFLISGAAETKNDKRTEIEPSLKVEADLAAIAWRKRLVN-ESGSCQ 328
            ++  R+  I+++E   I  +   +N     +  ++K  A   A  W   L + +  +C 
Sbjct: 242 NLLGMRRTCIMLMECLSILLSGLDRNCLAVVLSQNVKHRAKTIAEGWNPLLESLDMDACN 301

Query: 329 ASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGISKGLRCSHYLLTRIPAIVD 352
            + ++A   L  LA+F I   F  D+L  L+   + +  +  L  S  L  ++P +++
Sbjct: 302 GNSLEAHAFLQLLATFAIVADFKEDELLKLIPMVSRRRQAAELCRSLGLAEKMPGVIE 358

BLAST of Lsi01G020260 vs. TAIR 10
Match: AT4G14900.1 (FRIGIDA-like protein )

HSP 1 Score: 59.3 bits (142), Expect = 7.9e-09
Identity = 45/167 (26.95%), Postives = 72/167 (43.11%), Query Frame = 0

Query: 149 DFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLASLRHEIPLALKCA 208
           D G++D  +G+        LS L+ LC  M +RG   ++++   +L +LR +IP+AL   
Sbjct: 113 DTGEVDDGDGL--------LSALKSLCLKMDARGFWGFVIARKKELENLRSQIPVALVDC 172

Query: 209 PNPAKLVFDCIGRFY----LQGSKAYSKDSPMIPARQASILILELFLISGAAETKNDKRT 268
            +P KLV + +   +      G +  S D        A ++ILE  +           R 
Sbjct: 173 VDPPKLVLEAVSEVFPVDKRGGGEKVSNDFGW-----ACVVILESLIPVMVDPVMGKSRL 232

Query: 269 EIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLASFGI 312
            + PS+K +A   A  W+  L    G       D    L  L +FGI
Sbjct: 233 LVTPSVKEKAKEIAETWKASLEERGGIENVKTPDVHTFLQHLVTFGI 266

BLAST of Lsi01G020260 vs. TAIR 10
Match: AT1G31814.1 (FRIGIDA like 2 )

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-08
Identity = 62/246 (25.20%), Postives = 108/246 (43.90%), Query Frame = 0

Query: 79  SAIHAFQCRLNELQDHLGFIHNAIDARSKQHISLSSSSSIVATAESGRNPVSSSTHLSRS 138
           +A  +    +N++ +    +  A D   + H SL S S  ++ +E   +   SS   S  
Sbjct: 2   TAAESIAASINQIDEKKQKLKKAFD-DLQAHRSLLSPSFNLSWSEIDSH--FSSLQSSLF 61

Query: 139 PRSNLPESSSDFGKIDGEEGVNKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLASLR 198
            R     +SS+ G I+    V  +       EL   CE    +GL  Y++ +     S+ 
Sbjct: 62  NRLQSAVTSSNSGNIETPTAVTTE-TPVLWPELRKFCEKNDGKGLGNYMIENSRKRLSIN 121

Query: 199 HEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYSKDSPMIPARQASILILELFLISGAAET 258
            E+P A++C+ NPA LV D I   Y   S + S  +  I  ++  +L+LE  LI   A  
Sbjct: 122 EELPNAIRCSENPAPLVLDAIEGSYHCSSPSSSSSARAIDVKRIFVLLLEA-LIEINANL 181

Query: 259 KNDKRTEIEPSLKVEADLAAIAWRKRLVNESGSCQASDIDARGLLLFLASFGIPTVFTND 318
            ND        L+  A   A  W+  + N+         +A G L  +A+F + ++F+ +
Sbjct: 182 TND--------LRERARTIAYDWKPNIGNKPS-------EALGFLHLVAAFELGSLFSTE 227

Query: 319 DLRDLL 325
           ++ D +
Sbjct: 242 EICDYI 227