Lsi01G020180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G020180
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionalcohol dehydrogenase
Locationchr01: 27578420 .. 27581157 (+)
RNA-Seq ExpressionLsi01G020180
SyntenyLsi01G020180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATGTTGGTGAACATCAGAGCATTCATCTCTAAATTTTTAAGATTTGACATTTAAACAACATTCTAGGAGGACATTTAGCCCGGAAGTCTTCACAATTTCCAATCTGGTGTTTTTAACATTATATTTCTCCAGTTGCTTGCAGATAGCAAGGGCCTTTGGTGCTTCTCATATTATTGCCGTCGATGTTCAGGATGATAAACTGCTAAAAGCGAAGACAAGTGGTGCTACTCATACTATTAATTCTCGTAAAGAAGATGCTATTGAAAAGATAAGAGTATGTACTGTATGCCTTAATAAAAGTTTCCAAGATTCTGCTTAAGAACAAGTTATCGTACGTATCATGGATCATGGGAAACCAGTAATATGCACGGTAGATGGAATCATTATTTTGTTGTGTTTGATTATTATAATCTTTTCTTGGGTTCAATTTGGTATTTTTTTTTTTTTTTTTAACTAAGAGAATACCTTAGTCTCATGCTTTAAATTATTTACGTGTCCTGGTTTTCCTGCATCTCAATACTTCATCTAATTCAAAATTTTTGTATTTTTATAAAAGCTGTGTTTATATTTCAAAGTTTGGGAGAGAGAATTATTGATTCAAATGTATAACATGAGGTGTATTACTAAGTTGACTTGAATATAAGGTGTGGCGTTGGGTCTTCGAAATCCAAGCCTAGACAGAGACATGAAATATAACTAAGGACCAAACATCACTGGATCCCTTTCCAACTCTCTAAACACTCTATTATTCCGCTCATCCCAAAGATCCCACATAATTGCACACACCCCAATGAGCCAAAGTAACGACCTTCTCACGTAAGGGTAGATGGAACCAAATGTTTGAAAGAAATCACTCCAAATAGCTCTCACGAACTCGCAACTCCAAAGAATATTATCCAAGTCTTCTCCGTCCTTCGGTCAAAGAATACAACAAAAGGGATCAACAAAAGGTAAAGAACTTGTCTTTTCTTGGGATCTTTATCCTCCAAAGAGCAGAAAAATAGACTCACTTAAGGGGAAAGGATCTACAAGACTACGCTAGAAAGGATTACACTAGAATCCCTCCGAAGGGTTGGGACTCCATACCCTAAGATCATTTCTCCCTGGTCTAAAGACAATCGCCTCGAGCAAGGCCAAAAGAGATTGGACAAAGAGGGACAAAAACCAAACAAAAAAGACAAGGTGCTCCTTGACCCAACTAAAAAATCGAACACCACACAATTGTTGAAAGAAGACGAATGATAGACTTGGGAATGCCGGGCCACTGGTCTAAAATATCCTTCCTTCCGCCACCACTCAATGAACCAAAAGGGAGAAAGAAGGGAGCTCCTTAGAAATATCCTTCTATAAGTTTGACCTCACAAAGTTTAAATTTAGATCATTCCGGGCTTTTAGGGTATGAAAAGTGAAAGATTCTTTACATTTTTGTTTATGCAACTGGAGGGGTATTTTTTCTGTAATTCCATATTGCTGCAATGTAAGTGACTGAGTTGGTGACAGGAAATAACAGGAGGAATGGGAGTGGACATTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAGGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCGGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGGTCAGCCTTTTCTTCTTTTGTTTATGCTCTCTCCCTGAGGTAGCATTGATGCACGTTTTCAGCCTTTTCTTCTTTTGTTAATATGTATGCTGGGAGGGGAAAAGTGGGGAATTGTGTGAAGTATTTGGTACATATGTATGCTAGAGGGGCAAAATGGGGAATTGAGTGTAGTTAGTTAGAATGGTGCCTCATATAGGTAACAGAAGAGGGGTACGGAAAGGGTATCGGTTATTTTGGGAAGAGTAGCAATTTTGTTGGAGAGCAGCCCCCCCACGTGAGTTGGCTGGTATCGGTTATAAAGTCGTGTCTCGGAATTGATAATTAATGGAAGGCTAATTATATATTTTAGTTCTTAATGTTTATTCTCAATTTAGTCTTTATTTATTGTTTGAAGAGTACCAATTTCATCCCTTAAGGTTTGAGCATATTTCAATTTAGTCATTCATTGTTGGGGAAGGTTAAAAGTAGCATGATGAATAGAACTGCAGATAAAAAGGTAATCCAAGGTAAGAAAGTTCCTAAATCCAAGGTTAAAAAGATTATTATGTTTAACAAACAAGGCTCCATGAGCTGGAAGTTTTTGCCTCTATTTCTTTATGCACAATATGAACTGAATTTGTTAACAATGCGAGTTGGTGCAGATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTTCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGCGATGCGGTTTCTCGAAAGTACAAGTTCGAGGATTCAAACAAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGGGCTGTTATAGAGATTGCCTAATTGCTTCGCTGCCCCCAAATCACCACCTTTTAATCTTATGTAACTCTTCACATAAATATTATGTTACTTTCAAATAAGCCCTTATTTTTCTTGTGCCTCAAATGTCGATCTTGTCTTAGGCTTCCAAACACTATTTTCGCCCTTGTGATGTGTACTGTACATATTTAAGAAAAATTTATGAATCAAAGCCTGTTCAACTTTAGTTTGATTTGGAAAATTCTCTCTACC

mRNA sequence

ATGGGCATTTGCTTGCAGATAGCAAGGGCCTTTGGTGCTTCTCATATTATTGCCGTCGATGTTCAGGATGATAAACTGCTAAAAGCGAAGACAAGTGGTGCTACTCATACTATTAATTCTCGTAAAGAAGATGCTATTGAAAAGATAAGAGAAATAACAGGAGGAATGGGAGTGGACATTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAGGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCGGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGGTAACAGAAGAGGGGTACGGAAAGGGTATCGGTTATTTTGGGAAGAGTAGCAATTTTGTTGGAGAGCAGCCCCCCCACATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTTCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGCGATGCGGTTTCTCGAAAGTACAAGTTCGAGGATTCAAACAAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGGGCTGTTATAGAGATTGCCTAATTGCTTCGCTGCCCCCAAATCACCACCTTTTAATCTTATGTAACTCTTCACATAAATATTATGTTACTTTCAAATAAGCCCTTATTTTTCTTGTGCCTCAAATGTCGATCTTGTCTTAGGCTTCCAAACACTATTTTCGCCCTTGTGATGTGTACTGTACATATTTAAGAAAAATTTATGAATCAAAGCCTGTTCAACTTTAGTTTGATTTGGAAAATTCTCTCTACC

Coding sequence (CDS)

ATGGGCATTTGCTTGCAGATAGCAAGGGCCTTTGGTGCTTCTCATATTATTGCCGTCGATGTTCAGGATGATAAACTGCTAAAAGCGAAGACAAGTGGTGCTACTCATACTATTAATTCTCGTAAAGAAGATGCTATTGAAAAGATAAGAGAAATAACAGGAGGAATGGGAGTGGACATTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAGGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCGGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGGTAACAGAAGAGGGGTACGGAAAGGGTATCGGTTATTTTGGGAAGAGTAGCAATTTTGTTGGAGAGCAGCCCCCCCACATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTTCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGCGATGCGGTTTCTCGAAAGTACAAGTTCGAGGATTCAAACAAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGGGCTGTTATAGAGATTGCCTAA

Protein sequence

MGICLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKSSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLNEGKIIGRAVIEIA
Homology
BLAST of Lsi01G020180 vs. ExPASy Swiss-Prot
Match: A4YGN0 (Succinate-semialdehyde dehydrogenase (acetylating) OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=Msed_1424 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.9e-22
Identity = 65/189 (34.39%), Postives = 101/189 (53.44%), Query Frame = 0

Query: 5   LQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVEA 64
           +Q+ RA GA  IIAV  +  KL +A   GAT  +NS++ D ++ I+EITGG G  + +EA
Sbjct: 198 IQLLRASGAGRIIAVGTKKWKLDRAMELGATDVVNSKEIDPVKAIKEITGG-GPQVVIEA 257

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKSS 124
            G   T      SV+ GGK V++GL  A ++  I +  +VR                   
Sbjct: 258 GGNEDTIHMALDSVRIGGKVVLVGLPPATAMIPIRVASIVR------------------- 317

Query: 125 NFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLNE 184
                    I+V+G+YGGR R D+PKL++L   G ++ S  V+ +++ E+ N+A + L E
Sbjct: 318 -------GGIEVVGNYGGRPRVDMPKLLELVRQGRYDPSRLVTGRFRLEEINEAVKMLEE 359

Query: 185 GKIIGRAVI 194
           G+ I   +I
Sbjct: 378 GEAIRSLII 359

BLAST of Lsi01G020180 vs. ExPASy Swiss-Prot
Match: Q06004 (Sorbitol dehydrogenase OS=Bacillus subtilis (strain 168) OX=224308 GN=gutB PE=1 SV=3)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 48/121 (39.67%), Postives = 64/121 (52.89%), Query Frame = 0

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVEALGR 67
           A+AFGA  II  D++  +L  AK  GATH IN R++DA+E+I+ IT   GVD+A E  G 
Sbjct: 192 AKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQDALEEIKTITNDRGVDVAWETAGN 251

Query: 68  PQTFMQCTQSVKDGGKAVMIGLAQAGSV---------GEIDINRLVRRKVTEEGYGKGIG 120
           P        SV+ GGK  ++GL     +          EIDI  + R   T   Y KGI 
Sbjct: 252 PAALQSALASVRRGGKLAIVGLPSQNEIPLNVPFIADNEIDIYGIFRYANT---YPKGIE 309

BLAST of Lsi01G020180 vs. ExPASy Swiss-Prot
Match: P80094 (S-(hydroxymethyl)mycothiol dehydrogenase OS=Amycolatopsis methanolica OX=1814 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 52/190 (27.37%), Postives = 89/190 (46.84%), Query Frame = 0

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVEALGR 67
           AR  GA+ IIAVD    KL  A   GATHT+N+ + D +E ++ +TGG G D+ ++A+GR
Sbjct: 198 ARLAGANKIIAVDRDAKKLEWATELGATHTVNATETDVVEAVQALTGGFGADVVIDAVGR 257

Query: 68  PQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGK----S 127
           P+T+ Q   +    G  V++G+       E+ +                + +F +     
Sbjct: 258 PETWKQAFYARDLAGTVVLVGVPTPDMRLEMPL----------------LDFFSRGGALK 317

Query: 128 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 187
           S++ G+  P             +D P L+ L   G   L   V+ +   +D  KAF  ++
Sbjct: 318 SSWYGDCLP------------ERDFPVLIDLHLQGRLPLDKFVTERISLDDVEKAFHTMH 359

Query: 188 EGKIIGRAVI 194
            G+++   V+
Sbjct: 378 AGEVLRSVVV 359

BLAST of Lsi01G020180 vs. ExPASy Swiss-Prot
Match: A7Z4X0 (L-threonine 3-dehydrogenase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) OX=326423 GN=tdh PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 53/184 (28.80%), Postives = 86/184 (46.74%), Query Frame = 0

Query: 3   ICLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAV 62
           + + +A+A GAS I A+D  D +L  AK  GAT TI++ KED +E I  +TGG G+D+  
Sbjct: 183 MAVAVAKAAGASRIAAIDKNDYRLALAKKLGATITISADKEDPLEVIDTLTGGEGIDLVC 242

Query: 63  EALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGK 122
           E  G P    Q  +   +GG+  M+ L +          R V   +T +   KG      
Sbjct: 243 EMSGHPAAIAQGLKMAANGGRFHMLSLPE----------RPVTVDLTNDVVFKG------ 302

Query: 123 SSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDL 182
                      + V G  G +  +   ++  L ESG+ +L+  ++ +   ED  K F  +
Sbjct: 303 -----------LTVQGITGRKMFETWRQVSHLLESGLVDLTPVITHQLPLEDFEKGFDLM 339

Query: 183 NEGK 187
            +G+
Sbjct: 363 RKGQ 339

BLAST of Lsi01G020180 vs. ExPASy Swiss-Prot
Match: Q7NXH5 (L-threonine 3-dehydrogenase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=tdh PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 48/192 (25.00%), Postives = 93/192 (48.44%), Query Frame = 0

Query: 3   ICLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAV 62
           + + IA+  GA H++  DV D +L  AK  GAT  +N  +ED    ++E+    G D+ +
Sbjct: 178 MAVAIAKHVGARHVVITDVNDYRLELAKKMGATRAVNVAREDLKAVMQELHMSEGFDVGL 237

Query: 63  EALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGK 122
           E  G PQ F Q  +++  GGK  ++G+  + +   ID N+++                  
Sbjct: 238 EMSGNPQAFRQMLETMNHGGKVALLGIPPSNTA--IDWNQVI------------------ 297

Query: 123 SSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDL 182
              F G     +++ G YG    +   K+V L +SG+ ++S  ++  +K ++  + F  +
Sbjct: 298 ---FKG-----LEIKGIYGREMFETWYKMVALIQSGL-DISPIITHHFKVDEFEQGFAAM 339

Query: 183 NEGKIIGRAVIE 195
             G+  G+ +++
Sbjct: 358 LSGQ-SGKVILD 339

BLAST of Lsi01G020180 vs. ExPASy TrEMBL
Match: A0A0A0KXY5 (ADH_zinc_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337910 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 8.2e-79
Identity = 163/195 (83.59%), Postives = 167/195 (85.64%), Query Frame = 0

Query: 1   MGICLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDI 60
           MGI ++IARAFGASHIIAVDVQDDKLLKAKT GATHTINSRKEDAIEKIREITGGMGVD+
Sbjct: 1   MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV 60

Query: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYF 120
           AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK             
Sbjct: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK------------- 120

Query: 121 GKSSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQ 180
                        IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSN+AFQ
Sbjct: 121 -------------IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQ 169

Query: 181 DLNEGKIIGRAVIEI 196
           DLNEGKIIGRAVIEI
Sbjct: 181 DLNEGKIIGRAVIEI 169

BLAST of Lsi01G020180 vs. ExPASy TrEMBL
Match: A0A1S3BLX2 (alcohol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103491279 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.8e-78
Identity = 163/193 (84.46%), Postives = 165/193 (85.49%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGAS+IIAVDVQDDKLLKAKT GATHTINS KEDAIEKIREITGGMGVDIAVE
Sbjct: 257 CLQIARAFGASYIIAVDVQDDKLLKAKTIGATHTINSHKEDAIEKIREITGGMGVDIAVE 316

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK                
Sbjct: 317 ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK---------------- 376

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSN+AFQDLN
Sbjct: 377 ----------IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLN 423

Query: 184 EGKIIGRAVIEIA 197
           EGKIIGRAVIEIA
Sbjct: 437 EGKIIGRAVIEIA 423

BLAST of Lsi01G020180 vs. ExPASy TrEMBL
Match: A0A6J1E0R2 (alcohol dehydrogenase 1B OS=Momordica charantia OX=3673 GN=LOC111024875 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.2e-75
Identity = 155/192 (80.73%), Postives = 161/192 (83.85%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKT GATHTIN+RKEDAIEKIREIT GMGVDIAVE
Sbjct: 277 CLQIARAFGASHIIAVDVQDDKLLKAKTFGATHTINARKEDAIEKIREITEGMGVDIAVE 336

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLA++GSVGEIDINRLVRRK                
Sbjct: 337 ALGRPQTFMQCTQSVKDGGKAVMIGLAKSGSVGEIDINRLVRRK---------------- 396

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     +KVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSR Y FED+NKAFQDLN
Sbjct: 397 ----------LKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRTYNFEDANKAFQDLN 442

Query: 184 EGKIIGRAVIEI 196
           EG IIGRAVIE+
Sbjct: 457 EGNIIGRAVIEV 442

BLAST of Lsi01G020180 vs. ExPASy TrEMBL
Match: A0A6J1HTG6 (alcohol dehydrogenase 1 OS=Cucurbita maxima OX=3661 GN=LOC111466018 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.6e-74
Identity = 149/193 (77.20%), Postives = 163/193 (84.46%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHT+NS KEDAIEKI+EITGG GVDIAVE
Sbjct: 255 CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTVNSHKEDAIEKIKEITGGKGVDIAVE 314

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSV+DGGKAVMIGLA+ GSVGE+DINRLVRRK                
Sbjct: 315 ALGRPQTFMQCTQSVRDGGKAVMIGLAKHGSVGEVDINRLVRRK---------------- 374

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     I+V+GSYGGRARQDLPK++KLAESGIFNL+D VSRKYKFE++N+AFQDLN
Sbjct: 375 ----------IQVVGSYGGRARQDLPKVIKLAESGIFNLNDMVSRKYKFEEANEAFQDLN 421

Query: 184 EGKIIGRAVIEIA 197
           EGKIIGRAVIE+A
Sbjct: 435 EGKIIGRAVIEVA 421

BLAST of Lsi01G020180 vs. ExPASy TrEMBL
Match: A0A6J1EEI1 (uncharacterized protein LOC111432503 OS=Cucurbita moschata OX=3662 GN=LOC111432503 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.3e-73
Identity = 148/193 (76.68%), Postives = 162/193 (83.94%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKTSGATH +NS KEDAIEKI+EITGG GVDIAVE
Sbjct: 255 CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHAVNSLKEDAIEKIKEITGGKGVDIAVE 314

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSV+DGGKAVMIGLA+ GSVGE+DINRLVRRK                
Sbjct: 315 ALGRPQTFMQCTQSVRDGGKAVMIGLAKHGSVGEVDINRLVRRK---------------- 374

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     I+V+GSYGGRARQDLPK++KLAESGIFNL+D VSRKYKFE++N+AFQDLN
Sbjct: 375 ----------IQVVGSYGGRARQDLPKVIKLAESGIFNLNDMVSRKYKFEEANEAFQDLN 421

Query: 184 EGKIIGRAVIEIA 197
           EGKIIGRAVIE+A
Sbjct: 435 EGKIIGRAVIEVA 421

BLAST of Lsi01G020180 vs. NCBI nr
Match: XP_038903768.1 (alcohol dehydrogenase [Benincasa hispida])

HSP 1 Score: 307.8 bits (787), Expect = 6.9e-80
Identity = 165/193 (85.49%), Postives = 167/193 (86.53%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE
Sbjct: 277 CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 336

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK                
Sbjct: 337 ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK---------------- 396

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSN+AFQDLN
Sbjct: 397 ----------IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLN 443

Query: 184 EGKIIGRAVIEIA 197
           EGKI+GRAVIEIA
Sbjct: 457 EGKILGRAVIEIA 443

BLAST of Lsi01G020180 vs. NCBI nr
Match: XP_004142661.1 (uncharacterized protein LOC101207246 [Cucumis sativus] >KAE8649602.1 hypothetical protein Csa_012208 [Cucumis sativus])

HSP 1 Score: 304.3 bits (778), Expect = 7.6e-79
Identity = 163/192 (84.90%), Postives = 165/192 (85.94%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKT GATHTINSRKEDAIEKIREITGGMGVD+AVE
Sbjct: 257 CLQIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVE 316

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK                
Sbjct: 317 ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK---------------- 376

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSN+AFQDLN
Sbjct: 377 ----------IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLN 422

Query: 184 EGKIIGRAVIEI 196
           EGKIIGRAVIEI
Sbjct: 437 EGKIIGRAVIEI 422

BLAST of Lsi01G020180 vs. NCBI nr
Match: XP_008449387.1 (PREDICTED: alcohol dehydrogenase [Cucumis melo])

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-78
Identity = 163/193 (84.46%), Postives = 165/193 (85.49%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGAS+IIAVDVQDDKLLKAKT GATHTINS KEDAIEKIREITGGMGVDIAVE
Sbjct: 257 CLQIARAFGASYIIAVDVQDDKLLKAKTIGATHTINSHKEDAIEKIREITGGMGVDIAVE 316

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK                
Sbjct: 317 ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRK---------------- 376

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSN+AFQDLN
Sbjct: 377 ----------IKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLN 423

Query: 184 EGKIIGRAVIEIA 197
           EGKIIGRAVIEIA
Sbjct: 437 EGKIIGRAVIEIA 423

BLAST of Lsi01G020180 vs. NCBI nr
Match: XP_022158376.1 (alcohol dehydrogenase 1B [Momordica charantia])

HSP 1 Score: 290.8 bits (743), Expect = 8.7e-75
Identity = 155/192 (80.73%), Postives = 161/192 (83.85%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKT GATHTIN+RKEDAIEKIREIT GMGVDIAVE
Sbjct: 277 CLQIARAFGASHIIAVDVQDDKLLKAKTFGATHTINARKEDAIEKIREITEGMGVDIAVE 336

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSVKDGGKAVMIGLA++GSVGEIDINRLVRRK                
Sbjct: 337 ALGRPQTFMQCTQSVKDGGKAVMIGLAKSGSVGEIDINRLVRRK---------------- 396

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     +KVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSR Y FED+NKAFQDLN
Sbjct: 397 ----------LKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRTYNFEDANKAFQDLN 442

Query: 184 EGKIIGRAVIEI 196
           EG IIGRAVIE+
Sbjct: 457 EGNIIGRAVIEV 442

BLAST of Lsi01G020180 vs. NCBI nr
Match: XP_022966329.1 (alcohol dehydrogenase 1 [Cucurbita maxima])

HSP 1 Score: 287.7 bits (735), Expect = 7.4e-74
Identity = 149/193 (77.20%), Postives = 163/193 (84.46%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHT+NS KEDAIEKI+EITGG GVDIAVE
Sbjct: 255 CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTVNSHKEDAIEKIKEITGGKGVDIAVE 314

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALGRPQTFMQCTQSV+DGGKAVMIGLA+ GSVGE+DINRLVRRK                
Sbjct: 315 ALGRPQTFMQCTQSVRDGGKAVMIGLAKHGSVGEVDINRLVRRK---------------- 374

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     I+V+GSYGGRARQDLPK++KLAESGIFNL+D VSRKYKFE++N+AFQDLN
Sbjct: 375 ----------IQVVGSYGGRARQDLPKVIKLAESGIFNLNDMVSRKYKFEEANEAFQDLN 421

Query: 184 EGKIIGRAVIEIA 197
           EGKIIGRAVIE+A
Sbjct: 435 EGKIIGRAVIEVA 421

BLAST of Lsi01G020180 vs. TAIR 10
Match: AT5G63620.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 272.7 bits (696), Expect = 2.3e-73
Identity = 143/192 (74.48%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGAS IIAVDVQDDKL KAKT GATH +N+ KEDA+E+IREITGGMGVD+AVE
Sbjct: 261 CLQIARAFGASDIIAVDVQDDKLQKAKTLGATHIVNAAKEDAVERIREITGGMGVDVAVE 320

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALG+PQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRK                
Sbjct: 321 ALGKPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRK---------------- 380

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                     IKVIGSYGGRARQDLPK+VKLAESGIFNL++AVS KYKFED+ KAFQDLN
Sbjct: 381 ----------IKVIGSYGGRARQDLPKVVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLN 426

Query: 184 EGKIIGRAVIEI 196
           EGKI+ R V+EI
Sbjct: 441 EGKIVSRGVVEI 426

BLAST of Lsi01G020180 vs. TAIR 10
Match: AT5G63620.2 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 270.0 bits (689), Expect = 1.5e-72
Identity = 142/192 (73.96%), Postives = 155/192 (80.73%), Query Frame = 0

Query: 4   CLQIARAFGASHIIAVDVQDDKLLKAKTSGATHTINSRKEDAIEKIREITGGMGVDIAVE 63
           CLQIARAFGAS IIAVDVQDDKL KAKT GATH +N+ KEDA+E+IREITGGMGVD+AVE
Sbjct: 261 CLQIARAFGASDIIAVDVQDDKLQKAKTLGATHIVNAAKEDAVERIREITGGMGVDVAVE 320

Query: 64  ALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKVTEEGYGKGIGYFGKS 123
           ALG+PQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKV               
Sbjct: 321 ALGKPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKVW-------------- 380

Query: 124 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 183
                       VIGSYGGRARQDLPK+VKLAESGIFNL++AVS KYKFED+ KAFQDLN
Sbjct: 381 ------------VIGSYGGRARQDLPKVVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLN 426

Query: 184 EGKIIGRAVIEI 196
           EGKI+ R V+EI
Sbjct: 441 EGKIVSRGVVEI 426

BLAST of Lsi01G020180 vs. TAIR 10
Match: AT1G32780.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.0e-09
Identity = 54/193 (27.98%), Postives = 79/193 (40.93%), Query Frame = 0

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTSGATHTINSR--KEDAIEKIREITGGMGVDIAVEAL 67
           ARA GAS II VD    K  K K  G T  IN +   +   + IREITGG GVD + E  
Sbjct: 225 ARARGASRIIGVDANASKFEKGKLMGVTDFINPKDLTKPVHQMIREITGG-GVDYSFECT 284

Query: 68  GRPQTFMQCTQSVKDG-GKAVMIGLAQAGSVGEIDINRLV-RRKVTEEGYGKGIGYFGKS 127
           G      +   S   G G  V++G+        +    L   R++T   +G         
Sbjct: 285 GNVDVLREAFLSTHVGWGSTVLVGIYPTPRTLPLHPMELFDGRRITGSVFG--------- 344

Query: 128 SNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQDLN 187
                            G + +  LP   +    G+  L   ++ +  FE  N AFQ L 
Sbjct: 345 -----------------GFKPKSQLPNFAQQCMKGVVKLEPFITNELPFEKINDAFQLLR 389

Query: 188 EGKIIGRAVIEIA 197
           +GK + R +++I+
Sbjct: 405 DGKSL-RCILQIS 389

BLAST of Lsi01G020180 vs. TAIR 10
Match: AT4G22110.1 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.0e-09
Identity = 58/186 (31.18%), Postives = 81/186 (43.55%), Query Frame = 0

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTSGATHTINSR---KEDAIEKIREITGGMGVDIAVEA 67
           AR  GA  II VD+  +K    K  G T  INS    +    E I+E+TGG GVD + E 
Sbjct: 223 ARLRGAGKIIGVDLNPEKFELGKKFGFTDFINSTLCGENKISEVIKEMTGG-GVDYSFEC 282

Query: 68  LGRPQTFMQCTQSVKDG-GKAVMIGLAQ---AGSVGEIDINRLVRRKVTEEGYGKGIGYF 127
           +G P    +   S + G GK V++G+ +     S+G  D+ R               G  
Sbjct: 283 VGLPSLLTEAFSSTRTGSGKTVVLGIDKHLTPVSLGSFDLLR---------------GRH 342

Query: 128 GKSSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQ 187
              S F G +P             + D+P LV        NL   ++ + KFE+ NKAF 
Sbjct: 343 VCGSLFGGLKP-------------KLDIPILVDHYLKKELNLDSFITHELKFEEINKAFD 379

BLAST of Lsi01G020180 vs. TAIR 10
Match: AT4G22110.2 (GroES-like zinc-binding dehydrogenase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.0e-09
Identity = 58/186 (31.18%), Postives = 81/186 (43.55%), Query Frame = 0

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTSGATHTINSR---KEDAIEKIREITGGMGVDIAVEA 67
           AR  GA  II VD+  +K    K  G T  INS    +    E I+E+TGG GVD + E 
Sbjct: 223 ARLRGAGKIIGVDLNPEKFELGKKFGFTDFINSTLCGENKISEVIKEMTGG-GVDYSFEC 282

Query: 68  LGRPQTFMQCTQSVKDG-GKAVMIGLAQ---AGSVGEIDINRLVRRKVTEEGYGKGIGYF 127
           +G P    +   S + G GK V++G+ +     S+G  D+ R               G  
Sbjct: 283 VGLPSLLTEAFSSTRTGSGKTVVLGIDKHLTPVSLGSFDLLR---------------GRH 342

Query: 128 GKSSNFVGEQPPHIKVIGSYGGRARQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNKAFQ 187
              S F G +P             + D+P LV        NL   ++ + KFE+ NKAF 
Sbjct: 343 VCGSLFGGLKP-------------KLDIPILVDHYLKKELNLDSFITHELKFEEINKAFD 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A4YGN08.9e-2234.39Succinate-semialdehyde dehydrogenase (acetylating) OS=Metallosphaera sedula (str... [more]
Q060045.2e-1439.67Sorbitol dehydrogenase OS=Bacillus subtilis (strain 168) OX=224308 GN=gutB PE=1 ... [more]
P800945.2e-1427.37S-(hydroxymethyl)mycothiol dehydrogenase OS=Amycolatopsis methanolica OX=1814 PE... [more]
A7Z4X01.3e-1228.80L-threonine 3-dehydrogenase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6... [more]
Q7NXH53.8e-1225.00L-threonine 3-dehydrogenase OS=Chromobacterium violaceum (strain ATCC 12472 / DS... [more]
Match NameE-valueIdentityDescription
A0A0A0KXY58.2e-7983.59ADH_zinc_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337910 ... [more]
A0A1S3BLX21.8e-7884.46alcohol dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103491279 PE=4 SV=1[more]
A0A6J1E0R24.2e-7580.73alcohol dehydrogenase 1B OS=Momordica charantia OX=3673 GN=LOC111024875 PE=4 SV=... [more]
A0A6J1HTG63.6e-7477.20alcohol dehydrogenase 1 OS=Cucurbita maxima OX=3661 GN=LOC111466018 PE=4 SV=1[more]
A0A6J1EEI12.3e-7376.68uncharacterized protein LOC111432503 OS=Cucurbita moschata OX=3662 GN=LOC1114325... [more]
Match NameE-valueIdentityDescription
XP_038903768.16.9e-8085.49alcohol dehydrogenase [Benincasa hispida][more]
XP_004142661.17.6e-7984.90uncharacterized protein LOC101207246 [Cucumis sativus] >KAE8649602.1 hypothetica... [more]
XP_008449387.13.8e-7884.46PREDICTED: alcohol dehydrogenase [Cucumis melo][more]
XP_022158376.18.7e-7580.73alcohol dehydrogenase 1B [Momordica charantia][more]
XP_022966329.17.4e-7477.20alcohol dehydrogenase 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G63620.12.3e-7374.48GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT5G63620.21.5e-7273.96GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT1G32780.13.0e-0927.98GroES-like zinc-binding dehydrogenase family protein [more]
AT4G22110.13.0e-0931.18GroES-like zinc-binding dehydrogenase family protein [more]
AT4G22110.23.0e-0931.18GroES-like zinc-binding dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013149Alcohol dehydrogenase, C-terminalPFAMPF00107ADH_zinc_Ncoord: 4..109
e-value: 1.7E-24
score: 86.3
NoneNo IPR availableGENE3D3.90.180.10coord: 131..196
e-value: 8.4E-8
score: 34.2
NoneNo IPR availableGENE3D3.40.50.720coord: 4..112
e-value: 1.8E-30
score: 108.2
NoneNo IPR availablePANTHERPTHR43350:SF2NAD-DEPENDENT ALCOHOL DEHYDROGENASEcoord: 134..195
NoneNo IPR availablePANTHERPTHR43350:SF2NAD-DEPENDENT ALCOHOL DEHYDROGENASEcoord: 3..109
NoneNo IPR availablePANTHERPTHR43350NAD-DEPENDENT ALCOHOL DEHYDROGENASEcoord: 134..195
coord: 3..109
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 144..190
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G020180.1Lsi01G020180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010033 response to organic substance
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016491 oxidoreductase activity