Lsi01G020040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G020040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRanBP2-type domain-containing protein
Locationchr01: 26962587 .. 26966278 (-)
RNA-Seq ExpressionLsi01G020040
SyntenyLsi01G020040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTAGGTGGCAATCCAAGGCGGAGTTATTTTGAACTTCGAAACCCTTTCCCTCAAATACTCAACTTTGGTGCTTCATTCATTCTTTTTTGCTCTTTCAATTTCAACTGGTAAGATTCACAGTTTATTTCTCAATTTCTAGCTTTCAAACCCCAATTCTCTGATTTCTTCACTCGTCGATTTATTCATTTTTTGCAAAATATGGACTATCTTTTGCTCGAATCACGCTTGTTTAATCAACGTGGTGAATTTCTTTCTTCCTTTGTCGCATGTTCTTGGTTTCGTAATTTTACAATAGGAACAGTTGATGCTCTCTCGTTGTCCAAAATTATTGTTTGTTTGTAGTGTAGAGTTAGAATACGAGAGTATCTTTTCTCGTAGGTGTTGTTTTGAACTTTTGACATTTAATGATTGTGAGCTATGTGAATTTCCATACATGAGTTATTTTGTTTAAGTTATATGGACTCCTTTAAAATTAATTTTCATTTGGATTTGGAACGGTCTTGGAATTTGAGGATGTAATACTGCTAATTGCATTTCCAGTTATCCTGGAAATGTATAAATGTGAATAAAACGAGTTGTGGAAAAAGAAAGCATCAAAACTATCGATCAAAACAATCAAGAGTGGTTTAAAAGGTTAAATATCAATTAAGGATTTAGAATGTCAGGTTTCAAACCAAATATTTTGTGTATGCATAACCTTCTTGGCCTTTCCCTGTACATAATATAAAATCACAACCAATAAAAGGGCACATGCCAAAATCGACCTAATGACAAATCAGAGGTTGGAGAAACATATTTTTAACAACCCAGTGGGTGCAAAGCTAACAGTCATCTTCAGCTATGCGTAACTTGAGGAGTTGCTATGGCAACTCAGAAATGCATTGCTAAAAGCTTCTCTAGTGATGTTGGACTTTTTGTGACGACTCTTTGGTGTCACGGAATATATAACATTTTGTAATGTACTTTAGATGGATATCTTATGGTTGCAGACTTCTCTATGGCCATGAATAGAGGCTGTCAAATTATATATCCACCACTGAACAGCCTAATTTGCATCAGTTCACCAGGCCTTCTGTTGTGAAATTCTTTTATCATTTTATCTATAATTGTGGTAGCCATTCACTCGAAACTGTGAGACTTGGAGGGCCTCTTTACTCCACCCATTTCCCCCCACTTGAACCTTGATTATAATTTGGTACTGATTGCTTCCTGAAAGAGGTACTATGCTAATTTAAGAACAAGGGCTTGCTCCAGGCAGTTTGTACCCTAGTTATATATGTATTTCTTTCTGAAACAATATTTGCTTGAGTTGCAATGCATTAATTGCTTGTGACTTGTGCTGTTTTATCTAACTAGCTTTAGAGAATGCTGCGATTTCCTTTTTGTATAATTGTCCCTACTTTAACCATCTTGTTATTGGATACTACAGGAAATAAGCTCACGACAGAATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGGTGACTATTCCAGCTAATCATCTAATTCTTTGGTCCTGCTCCCTGTTTAAGACTAGGTAGCATTGTTTGTTTTATTTTTTAGTGCACTCTCTTTCTTAGGGTTAATTGGTGATAATTATAAGAATTGAATGATAGATCTTTCTGTTTAGGATCTTCCTATTTATTAACACATTATTAGGAAGCTCCTGAACAGAATGGCATGTTGTTTCCATGGGATTTAATGCGTTACTATCTCATTCAGAAGATTTCATATTCCTTTTTCGATCATCTATATAATAAATTTTGTAGTTTCCTTTGATACTGACTACCTGTTTCCTAGCATCATTATAACAAATAGGGTTTGTTTATATATATATATATATAGTTGGAAATTTTTCTTTCATTGAGAACAATGGAAGAAATCAGTGGATTCTATTTGAATTTGTATGCTTTCACTATAAGAAACGGTTCATGGTATGATGTTGAACATTACAAGATGCATCTAGATTGGTTTGCCCCCACATGACTGTGTAATTTGTGTCCTTACGAGTTGTCCTCAGAATGAGGTTATTGTATGATAGGCCTCGAATTATAGCTAACTCTGATTTTGAGAAAAATGAGAAGCTTTTAAAAGTTTTGATGCTTTGATTGTTTTACAATATCGTCAGGTTCTTGCTTTTTTAGTAGTGTATTCCTTTAATCTCCCTTGATAAGGATATACCTAGGAATTCAGAGCTCTTGTAGGATTATTTTCTTTTTGGTTATTTATCAATTTGTTTGTATGTCTTTTTTGAATGAGATAATGTGATATTGGCATTACAACACTGTCATAATATTGGTATCATTTGCATTTGCCAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGATGATAGAATAGATTGTTCTTTTTTCTCAGGCTACCAGGTATTTGCTTTTAGATGAGTAAATTGAAAGGCTAGCACTAGATATCTTGTTAATGAAGTTGTTTGCTTGATGTTAAAAAATAAAGTGATATGGAGCATGGAGCATGTGTTTAGGAACTAAGCAAATTTGATTGAATACAGATTGGAGAATCTTGTTCATAGGGGAGGAATTAATATAGTTTCTTTTCATCATTGTTTTTCTATATTACATTTGTATGCTTCTTTGTAATGGTTGCAATGACCCAACTTTTCTAAGATATCTCATTGGAGAAATTATTACATATATGACATTCATGATTGAAAACTTGGGTTTTTTTAATTAAAAAAGAAAAAAGAAAAAAATTGAACTGAACTGAACTTTATTTAAAAGGCGAGTAAAGGAAAATGTGCATGAACCTCATTAACAC

mRNA sequence

CGTAGGTGGCAATCCAAGGCGGAGTTATTTTGAACTTCGAAACCCTTTCCCTCAAATACTCAACTTTGGTGCTTCATTCATTCTTTTTTGCTCTTTCAATTTCAACTGGAAATAAGCTCACGACAGAATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGATGATAGAATAGATTGTTCTTTTTTCTCAGGCTACCAGGTATTTGCTTTTAGATGAGTAAATTGAAAGGCTAGCACTAGATATCTTGTTAATGAAGTTGTTTGCTTGATGTTAAAAAATAAAGTGATATGGAGCATGGAGCATGTGTTTAGGAACTAAGCAAATTTGATTGAATACAGATTGGAGAATCTTGTTCATAGGGGAGGAATTAATATAGTTTCTTTTCATCATTGTTTTTCTATATTACATTTGTATGCTTCTTTGTAATGGTTGCAATGACCCAACTTTTCTAAGATATCTCATTGGAGAAATTATTACATATATGACATTCATGATTGAAAACTTGGGTTTTTTTAATTAAAAAAGAAAAAAGAAAAAAATTGAACTGAACTGAACTTTATTTAAAAGGCGAGTAAAGGAAAATGTGCATGAACCTCATTAACAC

Coding sequence (CDS)

ATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGA

Protein sequence

MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHKELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQLSHHFGLTREEVRRRRLERFG
Homology
BLAST of Lsi01G020040 vs. ExPASy Swiss-Prot
Match: Q8RXW0 (Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-94
Identity = 186/324 (57.41%), Postives = 233/324 (71.91%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +++YYRL  + P+T  LLA NTL++LRP F+ P+IP I +VWFN HLI KH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           K+LKR  LS FYHV + HLVYNM+SLLWKGI+LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+ F+Y+R+Y+ EY+VGFSGVLFAMKVVL SQAE Y+ ++G+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L LKG+  G DP+T+ +R V R+++ PL F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQD----RLL 300
           R+R I G G V R QT           WRCQ+CT+DNS  LS CEMCG+ R       L 
Sbjct: 254 RRRRITGRGRVGRGQTG----IAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLN 313

Query: 301 SPHQLSHHFGLTREEVRRRRLERF 320
               LS    L  +E+RRRR+ERF
Sbjct: 314 QGPALSSSNDLPLDELRRRRVERF 333

BLAST of Lsi01G020040 vs. ExPASy Swiss-Prot
Match: Q8TEB9 (Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 101/322 (31.37%), Postives = 152/322 (47.20%), Query Frame = 0

Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHKELKRF 68
           ++SQ + +  N   P+T+  LA N    L P    P+  S      +     + K+ +R 
Sbjct: 15  LLSQIFHVGINNIPPVTLATLALNIWFFLNPQ--KPLYSSC----LSVEKCYQQKDWQRL 74

Query: 69  LLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLLV 128
           LLSP +H  D HL +NM S+LWKGI LE  +GS  FA ++ A  +++ G+  LL++  + 
Sbjct: 75  LLSPLHHADDWHLYFNMASMLWKGINLERRLGSRWFAYVITAFSVLT-GVVYLLLQFAVA 134

Query: 129 FFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMIS 188
            F  E  +    +VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + S
Sbjct: 135 EFMDEPDFKRSCAVGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFS 194

Query: 189 PGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRTI 248
           PG SF GHL GIL GL++           PL  ++ +     S  +G+     P RQ   
Sbjct: 195 PGTSFAGHLAGILVGLMYTQ--------GPLKKIMEACAGGFSSSVGY-----PGRQYYF 254

Query: 249 FGGGT-------VCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSP 308
              G+          R     E   + DT+   A   +   +    +    DR +   SP
Sbjct: 255 NSSGSSGYQDYYPHGRPDHYEEAPRNYDTY--TAGLSEEEQLERALQASLWDRGNTRNSP 312

Query: 309 HQLSHHFGLTREEVRRRRLERF 320
                H  L+ EE+RR+RL RF
Sbjct: 315 PPYGFH--LSPEEMRRQRLHRF 312

BLAST of Lsi01G020040 vs. ExPASy Swiss-Prot
Match: Q8BHC7 (Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-23
Identity = 71/199 (35.68%), Postives = 111/199 (55.78%), Query Frame = 0

Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRP-NFLHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ +++  N   P+T+  LA N    L P   L+    S+E  +       + K+ +R
Sbjct: 15  LLSQVFQIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKCY-------QQKDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG++LE  +GS  FA ++    L++ G+  LL++  +
Sbjct: 75  LLLSPLHHGDDWHLYFNMVSMLWKGVKLERRLGSRWFAYVIATFSLLT-GVVYLLLQFTV 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
                +  +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL+ I   
Sbjct: 135 AELLNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELVAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

BLAST of Lsi01G020040 vs. ExPASy Swiss-Prot
Match: Q5RBS4 (Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.7e-22
Identity = 70/198 (35.35%), Postives = 107/198 (54.04%), Query Frame = 0

Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHKELKRF 68
           ++SQ + +  N   P+T+  LA N    L P    P+  S      +     + ++ +R 
Sbjct: 15  LLSQIFHVGINNIPPVTLATLALNIWFFLNPQ--KPLYSSC----LSVEKCYQQRDWQRL 74

Query: 69  LLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLLV 128
           LLSP +H  D HL +N  S+LWKGI LE  +GS  FA ++    +++ G+  LL++  + 
Sbjct: 75  LLSPLHHADDWHLYFNTASVLWKGINLERRLGSRWFAYVITTFSVLT-GVVYLLLQFAVA 134

Query: 129 FFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMIS 188
            F  E  +    +VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + S
Sbjct: 135 EFMDEPDFKRSCAVGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFS 194

Query: 189 PGVSFLGHLGGILAGLLF 203
           PG SF GH  GIL GL++
Sbjct: 195 PGTSFAGHQAGILVGLMY 205

BLAST of Lsi01G020040 vs. ExPASy Swiss-Prot
Match: Q4V8F3 (Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.0e-22
Identity = 70/199 (35.18%), Postives = 107/199 (53.77%), Query Frame = 0

Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRP-NFLHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ + +  N   P+T+  LA N    L P   L+    S+E  +       +  + +R
Sbjct: 15  LLSQVFHIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKCY-------QQNDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG++LE  +GS  FA ++    L++ G+  LL++   
Sbjct: 75  LLLSPVHHGDDWHLYFNMVSMLWKGVKLEKRLGSRWFAYIIATFSLLT-GVVYLLLQFAS 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
                +  +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL  I   
Sbjct: 135 AELMNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELAAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

BLAST of Lsi01G020040 vs. ExPASy TrEMBL
Match: A0A5A7SVC3 (Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001640 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.7e-145
Identity = 268/320 (83.75%), Postives = 287/320 (89.69%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLILKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFA+KVVLGS+ ESYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSRPLGFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRC+ACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S     TREE+RRRRLERFG
Sbjct: 301 SSQLDFTREEMRRRRLERFG 314

BLAST of Lsi01G020040 vs. ExPASy TrEMBL
Match: A0A1S3B4I1 (rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.7e-145
Identity = 268/320 (83.75%), Postives = 287/320 (89.69%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLILKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFA+KVVLGS+ ESYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSRPLGFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRC+ACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S     TREE+RRRRLERFG
Sbjct: 301 SSQLDFTREEMRRRRLERFG 314

BLAST of Lsi01G020040 vs. ExPASy TrEMBL
Match: A0A0A0L173 (RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.1e-144
Identity = 267/320 (83.44%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFAMKVVLGS+ +SYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRCQACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S    LT EE+RRRRLERFG
Sbjct: 301 SSQLDLTWEEMRRRRLERFG 314

BLAST of Lsi01G020040 vs. ExPASy TrEMBL
Match: A0A6J1EL30 (rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111435540 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 8.5e-142
Identity = 259/320 (80.94%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV++QY RLQ  PITVGLLATNTLIHLRP FL  +IPSI+DVWFNA+LILK+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRF LSPFYHVGD HL YNMISLLWKGIQLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL FFNYE+ Y++EYSVGFSGVLFAMKVVLGSQ+ESYTY+HG+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           Q++SPGVSFLGHLGGILAG+LFL LKG NLG DPL VLIRSVGRILSRPLGFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNLGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
            R IFGGGTV RRQ       TS+DTWRC+ACTFDNSC+L  CEMCGTDRQDR +   +L
Sbjct: 241 HRRIFGGGTVGRRQ-------TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           SH F LT EE+RRRRLERFG
Sbjct: 301 SHRFDLTPEELRRRRLERFG 312

BLAST of Lsi01G020040 vs. ExPASy TrEMBL
Match: A0A6J1HXX5 (rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467237 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.5e-141
Identity = 259/320 (80.94%), Postives = 283/320 (88.44%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV++QY RLQ  PITVGLLATNTLIHLRP FL  +IPSI+DVWFNA+LILK+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRF LSPFYHVGD HL YNMISLLWKGIQLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL FFNYER Y++EYSVGFSGVLFAMKVVLGSQ+ESYTY+HG+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           Q++SPGVSFLGHLGGILAG+LFL LKG N G DPL VLIRSVGRILSRPLGFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
              IFGGGTV RRQ       TS+DTWRCQACTFDNSC+L  CEMCGTDRQDR +   +L
Sbjct: 241 HHRIFGGGTVGRRQ-------TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           SH F LT EE+RRRRLERFG
Sbjct: 301 SHRFDLTPEELRRRRLERFG 312

BLAST of Lsi01G020040 vs. NCBI nr
Match: XP_038881196.1 (rhomboid-like protein 14, mitochondrial [Benincasa hispida])

HSP 1 Score: 537.3 bits (1383), Expect = 8.7e-149
Identity = 278/320 (86.88%), Postives = 290/320 (90.62%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQRNPITVGLLATNTLIHLRPNFL  IIPSI+DVWFNAHLILKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQRNPITVGLLATNTLIHLRPNFLRHIIPSIDDVWFNAHLILKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKGIQLETSMGSIEFASMV ALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGIQLETSMGSIEFASMVSALLVMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLLV FNY+R Y+YEYSVGFSGVLFAMKVVLGSQAESYTYLHG+RVPSSHAAWLELILI
Sbjct: 121 KSLLV-FNYDRPYYYEYSVGFSGVLFAMKVVLGSQAESYTYLHGIRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G D LT+LIR  G+ILSRPLGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDLLTLLIRRAGQILSRPLGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSIDTWRCQACTFDNS +LSICEMCGTDRQDR+L  H L
Sbjct: 241 QRRIFGGGTVGSRP------ATSIDTWRCQACTFDNSGLLSICEMCGTDRQDRVLPLHHL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S  F LTREEVRRRRLERFG
Sbjct: 301 SRQFDLTREEVRRRRLERFG 313

BLAST of Lsi01G020040 vs. NCBI nr
Match: XP_008442110.1 (PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 rhomboid-like protein 14 [Cucumis melo var. makuwa] >TYK05460.1 rhomboid-like protein 14 [Cucumis melo var. makuwa])

HSP 1 Score: 525.4 bits (1352), Expect = 3.4e-145
Identity = 268/320 (83.75%), Postives = 287/320 (89.69%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLILKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFA+KVVLGS+ ESYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSRPLGFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRC+ACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S     TREE+RRRRLERFG
Sbjct: 301 SSQLDFTREEMRRRRLERFG 314

BLAST of Lsi01G020040 vs. NCBI nr
Match: XP_004146389.1 (rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhomboid-like protein 14, mitochondrial [Cucumis sativus] >KGN54804.1 hypothetical protein Csa_012505 [Cucumis sativus])

HSP 1 Score: 522.7 bits (1345), Expect = 2.2e-144
Identity = 267/320 (83.44%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFAMKVVLGS+ +SYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRCQACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S    LT EE+RRRRLERFG
Sbjct: 301 SSQLDLTWEEMRRRRLERFG 314

BLAST of Lsi01G020040 vs. NCBI nr
Match: XP_022928711.1 (rhomboid-like protein 14, mitochondrial [Cucurbita moschata] >XP_022928712.1 rhomboid-like protein 14, mitochondrial [Cucurbita moschata] >XP_022928713.1 rhomboid-like protein 14, mitochondrial [Cucurbita moschata] >XP_022928714.1 rhomboid-like protein 14, mitochondrial [Cucurbita moschata])

HSP 1 Score: 513.1 bits (1320), Expect = 1.8e-141
Identity = 259/320 (80.94%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV++QY RLQ  PITVGLLATNTLIHLRP FL  +IPSI+DVWFNA+LILK+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRF LSPFYHVGD HL YNMISLLWKGIQLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL FFNYE+ Y++EYSVGFSGVLFAMKVVLGSQ+ESYTY+HG+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           Q++SPGVSFLGHLGGILAG+LFL LKG NLG DPL VLIRSVGRILSRPLGFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNLGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
            R IFGGGTV RRQ       TS+DTWRC+ACTFDNSC+L  CEMCGTDRQDR +   +L
Sbjct: 241 HRRIFGGGTVGRRQ-------TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           SH F LT EE+RRRRLERFG
Sbjct: 301 SHRFDLTPEELRRRRLERFG 312

BLAST of Lsi01G020040 vs. NCBI nr
Match: XP_022967849.1 (rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967852.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967853.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967854.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima])

HSP 1 Score: 511.5 bits (1316), Expect = 5.1e-141
Identity = 259/320 (80.94%), Postives = 283/320 (88.44%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV++QY RLQ  PITVGLLATNTLIHLRP FL  +IPSI+DVWFNA+LILK+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRF LSPFYHVGD HL YNMISLLWKGIQLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL FFNYER Y++EYSVGFSGVLFAMKVVLGSQ+ESYTY+HG+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           Q++SPGVSFLGHLGGILAG+LFL LKG N G DPL VLIRSVGRILSRPLGFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
              IFGGGTV RRQ       TS+DTWRCQACTFDNSC+L  CEMCGTDRQDR +   +L
Sbjct: 241 HHRIFGGGTVGRRQ-------TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           SH F LT EE+RRRRLERFG
Sbjct: 301 SHRFDLTPEELRRRRLERFG 312

BLAST of Lsi01G020040 vs. TAIR 10
Match: AT3G17611.1 (RHOMBOID-like protein 14 )

HSP 1 Score: 347.1 bits (889), Expect = 1.6e-95
Identity = 186/324 (57.41%), Postives = 233/324 (71.91%), Query Frame = 0

Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +++YYRL  + P+T  LLA NTL++LRP F+ P+IP I +VWFN HLI KH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           K+LKR  LS FYHV + HLVYNM+SLLWKGI+LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+ F+Y+R+Y+ EY+VGFSGVLFAMKVVL SQAE Y+ ++G+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L LKG+  G DP+T+ +R V R+++ PL F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQD----RLL 300
           R+R I G G V R QT           WRCQ+CT+DNS  LS CEMCG+ R       L 
Sbjct: 254 RRRRITGRGRVGRGQTG----IAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLN 313

Query: 301 SPHQLSHHFGLTREEVRRRRLERF 320
               LS    L  +E+RRRR+ERF
Sbjct: 314 QGPALSSSNDLPLDELRRRRVERF 333

BLAST of Lsi01G020040 vs. TAIR 10
Match: AT3G17611.2 (RHOMBOID-like protein 14 )

HSP 1 Score: 242.7 bits (618), Expect = 4.1e-64
Identity = 136/242 (56.20%), Postives = 171/242 (70.66%), Query Frame = 0

Query: 82  MISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLLVFFNYERSYFYEYSVGF 141
           M+SLLWKGI+LETSMGS EFASMV  L+ MSQG+TLLL KSLL+ F+Y+R+Y+ EY+VGF
Sbjct: 1   MMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLLAKSLLLLFDYDRAYYNEYAVGF 60

Query: 142 SGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLL 201
           SGVLFAMKVVL SQAE Y+ ++G+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG++
Sbjct: 61  SGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGII 120

Query: 202 FLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRTIFGGGTVCRRQTAALERT 261
           +L LKG+  G DP+T+ +R V R+++ PL F   +   R+R I G G V R QT      
Sbjct: 121 YLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTG----I 180

Query: 262 TSIDTWRCQACTFDNSCVLSICEMCGTDRQD----RLLSPHQLSHHFGLTREEVRRRRLE 320
                WRCQ+CT+DNS  LS CEMCG+ R       L     LS    L  +E+RRRR+E
Sbjct: 181 AGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVE 238

BLAST of Lsi01G020040 vs. TAIR 10
Match: AT3G17611.3 (RHOMBOID-like protein 14 )

HSP 1 Score: 153.3 bits (386), Expect = 3.3e-37
Identity = 87/176 (49.43%), Postives = 112/176 (63.64%), Query Frame = 0

Query: 148 MKVVLGSQAESYTYLHGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLFLHLKG 207
           MKVVL SQAE Y+ ++G+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG+++L LKG
Sbjct: 1   MKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGIIYLKLKG 60

Query: 208 TNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRTIFGGGTVCRRQTAALERTTSIDTW 267
           +  G DP+T+ +R V R+++ PL F   +   R+R I G G V R QT           W
Sbjct: 61  SYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTG----IAGPGIW 120

Query: 268 RCQACTFDNSCVLSICEMCGTDRQD----RLLSPHQLSHHFGLTREEVRRRRLERF 320
           RCQ+CT+DNS  LS CEMCG+ R       L     LS    L  +E+RRRR+ERF
Sbjct: 121 RCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVERF 172

BLAST of Lsi01G020040 vs. TAIR 10
Match: AT3G58460.1 (RHOMBOID-like protein 15 )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 52/160 (32.50%), Postives = 85/160 (53.12%), Query Frame = 0

Query: 48  DVWFNAHLILKHKELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGA 107
           +V F    I+   ++ RF  +  +H    H+++NM++L+  G +LE  MGS+    +   
Sbjct: 50  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 109

Query: 108 LLLMSQGITLLLIKSLL---VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQA--ESYTYL 167
           LL  +  +  LLI SL     F+ Y+     E ++GFSG+LF+M V+  S +   S +  
Sbjct: 110 LLATTNAVLHLLIASLAGYNPFYQYDH-LMNECAIGFSGILFSMIVIETSLSGVTSRSVF 169

Query: 168 HGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLF 203
               VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 170 GLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 207

BLAST of Lsi01G020040 vs. TAIR 10
Match: AT3G58460.2 (RHOMBOID-like protein 15 )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 52/160 (32.50%), Postives = 85/160 (53.12%), Query Frame = 0

Query: 48  DVWFNAHLILKHKELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGA 107
           +V F    I+   ++ RF  +  +H    H+++NM++L+  G +LE  MGS+    +   
Sbjct: 73  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 132

Query: 108 LLLMSQGITLLLIKSLL---VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQA--ESYTYL 167
           LL  +  +  LLI SL     F+ Y+     E ++GFSG+LF+M V+  S +   S +  
Sbjct: 133 LLATTNAVLHLLIASLAGYNPFYQYDH-LMNECAIGFSGILFSMIVIETSLSGVTSRSVF 192

Query: 168 HGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLF 203
               VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 193 GLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXW02.2e-9457.41Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14... [more]
Q8TEB91.4e-2431.37Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1[more]
Q8BHC71.6e-2335.68Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1[more]
Q5RBS41.7e-2235.35Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1[more]
Q4V8F35.0e-2235.18Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SVC31.7e-14583.75Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3B4I11.7e-14583.75rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 ... [more]
A0A0A0L1731.1e-14483.44RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850... [more]
A0A6J1EL308.5e-14280.94rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HXX52.5e-14180.94rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467... [more]
Match NameE-valueIdentityDescription
XP_038881196.18.7e-14986.88rhomboid-like protein 14, mitochondrial [Benincasa hispida][more]
XP_008442110.13.4e-14583.75PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 ... [more]
XP_004146389.12.2e-14483.44rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhombo... [more]
XP_022928711.11.8e-14180.94rhomboid-like protein 14, mitochondrial [Cucurbita moschata] >XP_022928712.1 rho... [more]
XP_022967849.15.1e-14180.94rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomb... [more]
Match NameE-valueIdentityDescription
AT3G17611.11.6e-9557.41RHOMBOID-like protein 14 [more]
AT3G17611.24.1e-6456.20RHOMBOID-like protein 14 [more]
AT3G17611.33.3e-3749.43RHOMBOID-like protein 14 [more]
AT3G58460.11.8e-1132.50RHOMBOID-like protein 15 [more]
AT3G58460.21.8e-1132.50RHOMBOID-like protein 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 58..203
e-value: 1.1E-19
score: 70.9
NoneNo IPR availableGENE3D2.30.30.380coord: 261..318
e-value: 3.9E-8
score: 35.0
NoneNo IPR availablePANTHERPTHR43066RHOMBOID-RELATED PROTEINcoord: 1..318
NoneNo IPR availablePANTHERPTHR43066:SF1RHOMBOID PROTEIN 2coord: 1..318
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 12..209
e-value: 5.7E-22
score: 80.3
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 18..203
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 267..286
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 263..292
score: 8.072433
IPR036443Zinc finger, RanBP2-type superfamilySUPERFAMILY90209Ran binding protein zinc finger-likecoord: 265..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G020040.1Lsi01G020040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004252 serine-type endopeptidase activity