Lsi01G019560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G019560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase RNF126-like
Location	chr01: 25407114 .. 25407788 (-)
RNA-Seq Expression	Lsi01G019560
Synteny	Lsi01G019560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCCCGTACAACTATGTCCCTCCGATATTGTTTTCGCAGCAACTCCAACGGCAGCGTGGCCACCGACGATCAACCATTGCAATTCGTCGAACTCCGATTGAGGAGTTTCAGCGAATTAATGTGTACAACTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCGTTCCCTCTGCGAGAGCTTGAAGACCCACCCTTCCTCTCCCACTTCTTCCTCCTTCGCTTTCTCTCTTCCTTCCATCTCCCCGCCGCCGTTTGTGACGTCATCTTCCAGAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTCAATCTCAACTTCCATATCATCGCCGCTGTCGATTTTGTGCATACGCTGTGGGTGGACCTGGACCCAGCAGTGCTTGTTCCACAAGATTGGTCGCCGGTGAGGAAAGGGGCGCCGGCGTGGGTGATGGAGCGGGTAATGAAGGAGAAATATGATCGGAATGAGGGAGAAGAGGAAGGAGATCATTGCAGTGTTTGTTACGAGGATCTCCATGGGGATGGTGAAAAGGAGGAGGTGACAAGAATAGCGTGTGGGCATGTGTATCACAAGTCGTGCATCCTGAAATGGCTAAACATAAATAATTCATGTCCTTTGTGCAGAAGGGAATTAGAATGA

mRNA sequence

ATGTCTTCCCGTACAACTATGTCCCTCCGATATTGTTTTCGCAGCAACTCCAACGGCAGCGTGGCCACCGACGATCAACCATTGCAATTCGTCGAACTCCGATTGAGGAGTTTCAGCGAATTAATGTGTACAACTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCGTTCCCTCTGCGAGAGCTTGAAGACCCACCCTTCCTCTCCCACTTCTTCCTCCTTCGCTTTCTCTCTTCCTTCCATCTCCCCGCCGCCGTTTGTGACGTCATCTTCCAGAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTCAATCTCAACTTCCATATCATCGCCGCTGTCGATTTTGTGCATACGCTGTGGGTGGACCTGGACCCAGCAGTGCTTGTTCCACAAGATTGGTCGCCGGTGAGGAAAGGGGCGCCGGCGTGGGTGATGGAGCGGGTAATGAAGGAGAAATATGATCGGAATGAGGGAGAAGAGGAAGGAGATCATTGCAGTGTTTGTTACGAGGATCTCCATGGGGATGGTGAAAAGGAGGAGGTGACAAGAATAGCGTGTGGGCATGTGTATCACAAGTCGTGCATCCTGAAATGGCTAAACATAAATAATTCATGTCCTTTGTGCAGAAGGGAATTAGAATGA

Coding sequence (CDS)

ATGTCTTCCCGTACAACTATGTCCCTCCGATATTGTTTTCGCAGCAACTCCAACGGCAGCGTGGCCACCGACGATCAACCATTGCAATTCGTCGAACTCCGATTGAGGAGTTTCAGCGAATTAATGTGTACAACTTCTCACCAAATCCTCCAACAAACTTCCCCAACTCTAATTTCCGATGCTCTGTTTCCGTTCCCTCTGCGAGAGCTTGAAGACCCACCCTTCCTCTCCCACTTCTTCCTCCTTCGCTTTCTCTCTTCCTTCCATCTCCCCGCCGCCGTTTGTGACGTCATCTTCCAGAAGATCGCCTCCTTCGCCCTCCGAATGGCCGCCGGAAACCTCAATCTCAACTTCCATATCATCGCCGCTGTCGATTTTGTGCATACGCTGTGGGTGGACCTGGACCCAGCAGTGCTTGTTCCACAAGATTGGTCGCCGGTGAGGAAAGGGGCGCCGGCGTGGGTGATGGAGCGGGTAATGAAGGAGAAATATGATCGGAATGAGGGAGAAGAGGAAGGAGATCATTGCAGTGTTTGTTACGAGGATCTCCATGGGGATGGTGAAAAGGAGGAGGTGACAAGAATAGCGTGTGGGCATGTGTATCACAAGTCGTGCATCCTGAAATGGCTAAACATAAATAATTCATGTCCTTTGTGCAGAAGGGAATTAGAATGA

Protein sequence

MSSRTTMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE

Homology

[Close]

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 21/48 (43.75%), Postives = 36/48 (75.00%), Query Frame = 0


Query: 176 CSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           CS+C++D   D   E V ++ C H+YH++CI+ WLN++++CP+CR+ L
Sbjct: 253 CSICWDDFKID---ETVRKLPCSHLYHENCIVPWLNLHSTCPICRKSL 297

[Close]

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.9e-08
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 172 EGDHCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           E + C+VC +D     E  E  ++ C H+YHK C+L WL ++NSCP+CR EL
Sbjct: 220 EFNQCAVCMDDFE---EGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

[Close]

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.8e-08
Identity = 26/72 (36.11%), Postives = 46/72 (63.89%), Query Frame = 0


Query: 156 MERVMKEKYDRNEGEEEGD----HCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLN 215
           ++R +KE +D  E           C +CY+D++ + EK + T++ CGH++ K+C+ KWL 
Sbjct: 83  VKRAVKEAWDSFEPLSNDQLMDLTCPICYDDMNENDEK-QATKMPCGHIFGKNCLQKWLE 142

Query: 216 INNSCPLCRREL 224
            + +CPLCR+E+
Sbjct: 143 NHCTCPLCRKEV 153

[Close]

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-07
Identity = 24/49 (48.98%), Postives = 29/49 (59.18%), Query Frame = 0


Query: 175 HCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           HC+VC E+      K     + C H+YH  CIL WL I NSCP+CR EL
Sbjct: 200 HCAVCKENF---VLKSSAREMPCNHIYHPDCILPWLAIRNSCPVCRHEL 245

[Close]

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot
Match: Q7ZW78 (E3 ubiquitin-protein ligase RNF181 OS=Danio rerio OX=7955 GN=rnf181 PE=2 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 1.9e-07
Identity = 31/92 (33.70%), Postives = 43/92 (46.74%), Query Frame = 0


Query: 132 VDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHGDGEKEE 191
           +D+D       DW        A  + + +       E  ++G  C VC  +     E+E 
Sbjct: 35  LDIDSGSFDLSDWDQRLPPPAAKAVVQSLPVVIISPEQADKGVKCPVCLLEFE---EQES 94

Query: 192 VTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           V  + C H++H  CIL WLN  NSCPLCR EL
Sbjct: 95  VREMPCKHLFHTGCILPWLNKTNSCPLCRLEL 123

[Close]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL
Match: A0A0A0LRB0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.4e-77
Identity = 154/225 (68.44%), Postives = 177/225 (78.67%), Query Frame = 0


Query: 1   MSSRTTMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISD 60
           MSS TT S R   R N+NGS AT+ QPLQFVELRL  FS+L+ + SHQILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  ALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHI 120
            L  FPLRELEDPPF+SH  L RFLSS+++  AVCD IF KI+SFA ++ AGNLN NFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCY 180
           IAA+DFV+T WVDLDPA    +D + VR+GAP   +ER+MKEKYD N GEE  D CSVCY
Sbjct: 121 IAALDFVYTHWVDLDPA----EDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180

Query: 181 EDLHGDGEKE-EVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           EDLHG  EKE EV+RI CGH++HKSCILKWL I+NSCPLCR ELE
Sbjct: 181 EDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL
Match: A0A0A0L3Z2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.0e-69
Identity = 149/234 (63.68%), Postives = 169/234 (72.22%), Query Frame = 0


Query: 1   MSSRTTMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISD 60
           MS   T S +   R N   S+ATD QPLQFV+LRLR F +L+ T S QILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  ALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFA---LRMAAGNLNLN 120
            L PF L ELEDPPF+S+FFL RFLSS+ + AAVCD IF  I+SFA   L  A  N+N+N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEE--GDH 180
           FHIIA VD V+T WVDLDPA    +D   VRKGA   V+ERVMKEKYD N GEE+   D 
Sbjct: 121 FHIIADVDIVYTHWVDLDPA----EDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQ 180

Query: 181 CSVCYEDLHGDGEKE-----EVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           CSVCYE L+G  E E     EV+RI CGH+YHKSCILKWL I+NSCPLCRRELE
Sbjct: 181 CSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL
Match: A0A6J1G809 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111451600 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.8e-58
Identity = 121/219 (55.25%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 6   TMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPF 65
           ++S +  FR+NS G++A+ +QP+QFVELRL   S L+  +S QILQQT  T + DALFPF
Sbjct: 8   SLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPMGDALFPF 67

Query: 66  PLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVD 125
            LR+L  PPFLS   +LRFLSSF +P AVC++IF +I+SFAL +AAGN++LNFHI+  VD
Sbjct: 68  ILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNFHIVVDVD 127

Query: 126 FVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHG 185
            +  +WVDLDP  L+ +   P   GAPA  +ER+ ++++D    EEEG+ CSVC E L G
Sbjct: 128 AIEMIWVDLDP-FLIEEYSPPAMNGAPASAIERLQRQEFDGLREEEEGE-CSVCCEALKG 187

Query: 186 DGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           +   EEV+RI CGHVYHK CIL+WL ++NSCPLCR +LE
Sbjct: 188 E---EEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLE 221

[Close]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.8e-58
Identity = 116/219 (52.97%), Postives = 163/219 (74.43%), Query Frame = 0


Query: 7   MSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPFP 66
           +S +   +S  NG+VA+D QP+Q +ELRL + + L+  +S QILQQT  T + DALFPF 
Sbjct: 9   LSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGDALFPFV 68

Query: 67  LRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVDF 126
           LR+L+ PPFLS+ ++L+FLSSF +P A CD+I+++I+SFAL++AAGN++LNFHI+  VD 
Sbjct: 69  LRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHIVVEVDV 128

Query: 127 VHTLWVDLDPAVLVPQDWSPVR-KGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHG 186
           +  +WVDLDP ++  +++SP    GAPA  +ER+ ++++D    EEE   CSVC E L G
Sbjct: 129 IEMIWVDLDPFLV--EEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKG 188

Query: 187 DGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           +   EEV+RI CGHVYHK CILKWL ++NSCPLCR +LE
Sbjct: 189 E---EEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLE 222

[Close]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL
Match: A0A6J1L387 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 3.1e-58
Identity = 116/219 (52.97%), Postives = 158/219 (72.15%), Query Frame = 0


Query: 6   TMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPF 65
           ++S ++  R+N+ G++A+ + P+QFVELRL   S L+  +S QILQQ+  T + ++LFPF
Sbjct: 8   SLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPLGESLFPF 67

Query: 66  PLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVD 125
            LR+LE PPFL ++ +L FLSSF +P A CD+I+Q+I+SFALR+ A N++LNFHI+  VD
Sbjct: 68  ILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNFHIVVDVD 127

Query: 126 FVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHG 185
            +  +WVDLDP  LV +   P   GAPA  +ER+ ++K+D    EEE   CSVC E L G
Sbjct: 128 AIEMIWVDLDP-FLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVCCEALRG 187

Query: 186 DGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           +   EEV+RI CGHVYHK CILKWL I+NSCPLCR +LE
Sbjct: 188 E---EEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLE 222

[Close]

BLAST of Lsi01G019560 vs. NCBI nr
Match: XP_011650219.1 (RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein Csa_022479 [Cucumis sativus])

HSP 1 Score: 299.3 bits (765), Expect = 2.8e-77
Identity = 154/225 (68.44%), Postives = 177/225 (78.67%), Query Frame = 0


Query: 1   MSSRTTMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISD 60
           MSS TT S R   R N+NGS AT+ QPLQFVELRL  FS+L+ + SHQILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  ALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHI 120
            L  FPLRELEDPPF+SH  L RFLSS+++  AVCD IF KI+SFA ++ AGNLN NFHI
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNLNSNFHI 120

Query: 121 IAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCY 180
           IAA+DFV+T WVDLDPA    +D + VR+GAP   +ER+MKEKYD N GEE  D CSVCY
Sbjct: 121 IAALDFVYTHWVDLDPA----EDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCY 180

Query: 181 EDLHGDGEKE-EVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           EDLHG  EKE EV+RI CGH++HKSCILKWL I+NSCPLCR ELE
Sbjct: 181 EDLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of Lsi01G019560 vs. NCBI nr
Match: XP_031740500.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 2.2e-69
Identity = 149/234 (63.68%), Postives = 169/234 (72.22%), Query Frame = 0


Query: 1   MSSRTTMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISD 60
           MS   T S +   R N   S+ATD QPLQFV+LRLR F +L+ T S QILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  ALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFA---LRMAAGNLNLN 120
            L PF L ELEDPPF+S+FFL RFLSS+ + AAVCD IF  I+SFA   L  A  N+N+N
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEE--GDH 180
           FHIIA VD V+T WVDLDPA    +D   VRKGA   V+ERVMKEKYD N GEE+   D 
Sbjct: 121 FHIIADVDIVYTHWVDLDPA----EDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQ 180

Query: 181 CSVCYEDLHGDGEKE-----EVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           CSVCYE L+G  E E     EV+RI CGH+YHKSCILKWL I+NSCPLCRRELE
Sbjct: 181 CSVCYEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 230

[Close]

BLAST of Lsi01G019560 vs. NCBI nr
Match: XP_023533113.1 (RING-H2 finger protein ATL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.4 bits (607), Expect = 5.9e-59
Identity = 121/220 (55.00%), Postives = 163/220 (74.09%), Query Frame = 0


Query: 6   TMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPF 65
           ++S +  FR+N+ G++A+ +QP+QFVELRL   S L+  +S QILQQT  T + DALFPF
Sbjct: 8   SLSFQCSFRTNTTGNLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPMGDALFPF 67

Query: 66  PLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVD 125
            LR+L DPPFLS   +LRFLSSF +P AVC++IF +I+SFAL +AAGN++LNFHI+  VD
Sbjct: 68  ILRDLLDPPFLSKSHVLRFLSSFTIPIAVCNIIFAEISSFALHLAAGNVDLNFHIVVDVD 127

Query: 126 FVHTLWVDLDPAVLVPQDWSP-VRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLH 185
            +  +WVDLDP ++  +++SP    GAPA  +ER+ ++K+D    EE+   CSVC E L 
Sbjct: 128 AIEMIWVDLDPFLI--EEYSPAAMNGAPASAIERLQRQKFDGLREEEKEGDCSVCCEALK 187

Query: 186 GDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           G+   EEV+RI CGHVYHK CILKWL ++NSCPLCR +LE
Sbjct: 188 GE---EEVSRIPCGHVYHKCCILKWLEMSNSCPLCRTQLE 222

[Close]

BLAST of Lsi01G019560 vs. NCBI nr
Match: KAG6605221.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.1 bits (601), Expect = 2.9e-58
Identity = 116/219 (52.97%), Postives = 163/219 (74.43%), Query Frame = 0


Query: 7   MSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPFP 66
           +S +   +S  NG+VA+D QP+Q +ELRL + + L+  +S QILQQT  T + DALFPF 
Sbjct: 9   LSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGDALFPFV 68

Query: 67  LRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVDF 126
           LR+L+ PPFLS+ ++L+FLSSF +P A CD+I+++I+SFAL++AAGN++LNFHI+  VD 
Sbjct: 69  LRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHIVVEVDV 128

Query: 127 VHTLWVDLDPAVLVPQDWSPVR-KGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHG 186
           +  +WVDLDP ++  +++SP    GAPA  +ER+ ++++D    EEE   CSVC E L G
Sbjct: 129 IEMIWVDLDPFLV--EEYSPAEMNGAPASAIERLQRQEFDGLRDEEEEGDCSVCCEALKG 188

Query: 187 DGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           +   EEV+RI CGHVYHK CILKWL ++NSCPLCR +LE
Sbjct: 189 E---EEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLE 222

[Close]

BLAST of Lsi01G019560 vs. NCBI nr
Match: XP_022947840.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 235.7 bits (600), Expect = 3.8e-58
Identity = 121/219 (55.25%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 6   TMSLRYCFRSNSNGSVATDDQPLQFVELRLRSFSELMCTTSHQILQQTSPTLISDALFPF 65
           ++S +  FR+NS G++A+ +QP+QFVELRL   S L+  +S QILQQT  T + DALFPF
Sbjct: 8   SLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPMGDALFPF 67

Query: 66  PLRELEDPPFLSHFFLLRFLSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVD 125
            LR+L  PPFLS   +LRFLSSF +P AVC++IF +I+SFAL +AAGN++LNFHI+  VD
Sbjct: 68  ILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNFHIVVDVD 127

Query: 126 FVHTLWVDLDPAVLVPQDWSPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHG 185
            +  +WVDLDP  L+ +   P   GAPA  +ER+ ++++D    EEEG+ CSVC E L G
Sbjct: 128 AIEMIWVDLDP-FLIEEYSPPAMNGAPASAIERLQRQEFDGLREEEEGE-CSVCCEALKG 187

Query: 186 DGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRRELE 225
           +   EEV+RI CGHVYHK CIL+WL ++NSCPLCR +LE
Sbjct: 188 E---EEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLE 221

[Close]

BLAST of Lsi01G019560 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-10
Identity = 40/139 (28.78%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 85  LSSFHLPAAVCDVIFQKIASFALRMAAGNLNLNFHIIAAVDFVHTLWVDLDPAVLVPQDW 144
           L  +   + +CD +  KI++ A+ +  G+      + A V +  T+ V            
Sbjct: 80  LVGYPFESYLCDDLAPKISTAAIDLGFGHNGFTLTMAATVTY-RTVSV-----------M 139

Query: 145 SPVRKGAPAWVMERVMKEKYDRNEGEEEGDHCSVCYEDLHGDGEKEEVTRIACGHVYHKS 204
           S   K     ++ R+  E++   + E E   CS+C E L    +  +VTR+ C HV+H  
Sbjct: 140 SNDEKSLRMVLLGRMKAEEFKSLKMETE--PCSICLESLVSGPKPRDVTRMTCSHVFHNG 199

Query: 205 CILKWLNINNSCPLCRREL 224
           C+L+WL   N+CPLCR E+
Sbjct: 200 CLLEWLKRKNTCPLCRTEI 204

[Close]

BLAST of Lsi01G019560 vs. TAIR 10
Match: AT4G12190.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-10
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 172 EGDHCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           E + CS+C E L    +  ++TR+ C HV+H  C+L+WL   N+CPLCR EL
Sbjct: 18  ETEPCSICLESLVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

[Close]

BLAST of Lsi01G019560 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-10
Identity = 24/48 (50.00%), Postives = 33/48 (68.75%), Query Frame = 0


Query: 176 CSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSCPLCRREL 224
           C+VC E+    G   + T + C H+YHK CI+ WL +NNSCP+CRR+L
Sbjct: 224 CTVCMEEFIVGG---DATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

[Close]

BLAST of Lsi01G019560 vs. TAIR 10
Match: AT4G12210.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 7.0e-10
Identity = 50/187 (26.74%), Postives = 81/187 (43.32%), Query Frame = 0


Query: 44  TTSHQILQQTSPTL-------ISDALFPFPLRELEDPPFLSHFFLLRFLSSFHLPAAVCD 103
           TT HQ  + T  TL       +S +     +R L    F SH +L             CD
Sbjct: 42  TTDHQTRRSTPATLSCLDTINLSSSSHDH-IRSLLRTRFASHHWL-------------CD 101

Query: 104 VIFQKIASFALRMAAGNLNLNFHIIAAVDFVHTLWVDLDPAVLVPQDWSPVRKGAPAWVM 163
            +  +I++ A+ +  G       ++  V +  T+ V            S  +K     ++
Sbjct: 102 DLASEISTAAIDLGFGRNGFTITVVVTVTY-RTVSVT-----------SNNKKSLRIVLL 161

Query: 164 ERVMKEKYDRNEGEEEGDHCSVCYEDLHGDGEKEEVTRIACGHVYHKSCILKWLNINNSC 223
            R+  E+    + E E   CS+C E+L    +  ++TR+ C HV+H  C+L+W    N+C
Sbjct: 162 GRIKAEELKSLKMETES--CSICLENLVSGPKPSDLTRMTCSHVFHNPCLLEWFMRKNTC 200

[Close]

BLAST of Lsi01G019560 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 7.0e-10
Identity = 30/86 (34.88%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 138 VLVPQDWSPVRKGAPAWVMERVMKE-KYDRNEGEEEGDHCSVCYEDLHGDGEKEEVTRIA 197
           V+ P   S + +GA   V  R+++E + +  + EEE + CS+C E        E++ R+ 
Sbjct: 120 VIFPHGPSLLSRGASGEVFHRLVEEQRVESADLEEEDETCSICIEKF--SESHEDIIRVP 179

Query: 198 -CGHVYHKSCILKWLNINNSCPLCRR 222
            C H++H+ C+ +WL + NSCPLCR+
Sbjct: 180 DCLHLFHQGCLFEWLGLQNSCPLCRK 203

The following BLAST results are available for this feature:

BLAST of Lsi01G019560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9VHI7	1.7e-08	43.75	E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1...	[more]
P0CH30	2.9e-08	46.15	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9Y7K6	3.8e-08	36.11	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
O22283	1.1e-07	48.98	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q7ZW78	1.9e-07	33.70	E3 ubiquitin-protein ligase RNF181 OS=Danio rerio OX=7955 GN=rnf181 PE=2 SV=2	[more]

BLAST of Lsi01G019560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRB0	1.4e-77	68.44	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432790 P...	[more]
A0A0A0L3Z2	1.0e-69	63.68	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G551140 P...	[more]
A0A6J1G809	1.8e-58	55.25	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1G7Z5	1.8e-58	52.97	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1L387	3.1e-58	52.97	RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE...	[more]

BLAST of Lsi01G019560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650219.1	2.8e-77	68.44	RING-H2 finger protein ATL16 [Cucumis sativus] >KGN63362.1 hypothetical protein ...	[more]
XP_031740500.1	2.2e-69	63.68	E3 ubiquitin-protein ligase At1g63170-like [Cucumis sativus]	[more]
XP_023533113.1	5.9e-59	55.00	RING-H2 finger protein ATL1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605221.1	2.9e-58	52.97	E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022947840.1	3.8e-58	55.25	probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata]	[more]

BLAST of Lsi01G019560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05350.1	1.1e-10	28.78	RING/U-box superfamily protein	[more]
AT4G12190.1	1.4e-10	46.15	RING/U-box superfamily protein	[more]
AT1G60360.1	3.2e-10	50.00	RING/U-box superfamily protein	[more]
AT4G12210.1	7.0e-10	26.74	RING/U-box superfamily protein	[more]
AT5G37200.1	7.0e-10	34.88	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 176..219 e-value: 1.9E-7 score: 40.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 175..220 e-value: 2.1E-12 score: 47.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 176..220 score: 12.931868
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..224 e-value: 1.8E-19 score: 70.7
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 79..224
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 79..224
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 168..223

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G019560.1	Lsi01G019560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity