Homology
BLAST of Lsi01G019440 vs. ExPASy Swiss-Prot
Match:
Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)
HSP 1 Score: 422.9 bits (1086), Expect = 4.3e-117
Identity = 230/426 (53.99%), Postives = 292/426 (68.54%), Query Frame = 0
Query: 14 ALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGKNRLQRLNAMVL 73
A + + SR AL ++ GF++ L HVD KNLT+F+ L R + RG RLQRL AM+
Sbjct: 20 APTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLERAIERGSRRLQRLEAML- 79
Query: 74 AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQAT 133
N G V+ V AG+GE+LM L+IGTP + FSAIMDTGSDLIWTQC+PC QCF+Q+T
Sbjct: 80 --NGPSG--VETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQST 139
Query: 134 PIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDS 193
PIF+P+ SSSFS + CSS+LC AL + TCSNN C+Y Y YGD S T+G + ET TFG S
Sbjct: 140 PIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-S 199
Query: 194 SEDQNINF---ENL--FNKIQGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLL 253
NI F EN F + G GLVG+GRGPLSL SQL KF+YC+T I S PS+LL
Sbjct: 200 VSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLL 259
Query: 254 LGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELH-NDGS 313
LGSLAN T TT LI++ P+FYY++L G+SVG T+LPI S F L+ N+G+
Sbjct: 260 LGSLAN---SVTAGSPNTT-LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGT 319
Query: 314 GGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKL 373
GG+IIDSGTT+TY N A+ ++ EFI+Q+NLPV + + G DLCF P++ + +++P
Sbjct: 320 GGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTF 379
Query: 374 TFHFKGADLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLS 433
HF G DLELP ENY I S GLICLA+G SS+GMSIFGN+QQQN +VV+D +S
Sbjct: 380 VMHFDGGDLELPSENYFISPSN-GLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVS 434
BLAST of Lsi01G019440 vs. ExPASy Swiss-Prot
Match:
Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)
HSP 1 Score: 392.1 bits (1006), Expect = 8.1e-108
Identity = 205/424 (48.35%), Postives = 282/424 (66.51%), Query Frame = 0
Query: 16 SSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGKNRLQRLNAMVLAA 75
SST L K P G RV L VD KNLT++E ++R + RG+ R++ +NAM+ ++
Sbjct: 23 SSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSS 82
Query: 76 NAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPI 135
+ ++ PV AG+GE+LM +AIGTP SFSAIMDTGSDLIWTQC+PC QCF Q TPI
Sbjct: 83 SG-----IETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPI 142
Query: 136 FDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDSSE 195
F+P+ SSSFS + C S+ C LP+ TC+NN C+Y Y YGD S+T+G +A ETFTF ++S
Sbjct: 143 FNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTF-ETSS 202
Query: 196 DQNINF-----ENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLG 255
NI F F + G GL+G+G GPLSL SQL +F+YC+T+ S PS+L LG
Sbjct: 203 VPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALG 262
Query: 256 SLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELHNDGSGGV 315
S A+ P+ + +T LI + P++YY++LQGI+VG L IP STF+L +DG+GG+
Sbjct: 263 SAASGVPEGS----PSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGM 322
Query: 316 IIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFH 375
IIDSGTT+TY+ A+ + F Q+NLP D + GL CF P++ + V+VP+++
Sbjct: 323 IIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQ 382
Query: 376 FKGADLELPGENYMIGDSRAGLICLAIGSSR--GMSIFGNLQQQNFMVVHDLQEETLSFL 433
F G L L +N +I + G+ICLA+GSS G+SIFGN+QQQ V++DLQ +SF+
Sbjct: 383 FDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFV 435
BLAST of Lsi01G019440 vs. ExPASy Swiss-Prot
Match:
Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)
HSP 1 Score: 255.4 bits (651), Expect = 1.2e-66
Identity = 155/446 (34.75%), Postives = 235/446 (52.69%), Query Frame = 0
Query: 31 PSNGFRVKLNHVD----HVKNLTRFERLRRGVARGKNRLQRLN-AMVLAANAAVGDQVKA 90
P FR++L VD NLT E LRR + R + RL + A AA+A +
Sbjct: 21 PPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGIGMARGEAASARKAVVAET 80
Query: 91 PVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKKSSSFS 150
P++ GE+L+KL IGTPP F+A +DT SDLIWTQC+PC C+ Q P+F+P+ SS+++
Sbjct: 81 PIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTGCYHQVDPMFNPRVSSTYA 140
Query: 151 KISCSSELCGALPTSTCSNN---GCEYLYTYGDSSSTEGVLAFETFTFGDSSEDQNINFE 210
+ CSS+ C L C ++ C+Y YTY +++TEG LA + G+ + + + F
Sbjct: 141 ALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLAVDKLVIGEDA-FRGVAFG 200
Query: 211 NLFNKI------QGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIK 270
+ Q G+VGLGRGPLSLVSQL ++FAYCL P L+LG+ A+
Sbjct: 201 CSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAYCLPPPASRIPGKLVLGADADAA 260
Query: 271 PKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKST----------------- 330
T P+ R+P PS+YYL+L G+ +GD + +P +T
Sbjct: 261 RNAT--NRIAVPMRRDPRYPSYYYLNLDGLLIGDRAMSLPPTTTTTATATATAPAPAPTP 320
Query: 331 ------FELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCF 390
+ + G+IID +TIT++E + + L N+ ++ LP + GLDLCF
Sbjct: 321 SPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVNDLEVEIRLPRGTGSSLGLDLCF 380
Query: 391 NLP--AEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSR--GMSIFGNL 436
LP +V VP + F G L L D +G++CL +G + +SI GN
Sbjct: 381 ILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRESGMMCLMVGRAEAGSVSILGNF 440
BLAST of Lsi01G019440 vs. ExPASy Swiss-Prot
Match:
Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)
HSP 1 Score: 254.2 bits (648), Expect = 2.6e-66
Identity = 162/439 (36.90%), Postives = 235/439 (53.53%), Query Frame = 0
Query: 19 LSRRALQKPNKLPSNGFRVKLNHVDHVKN------LTRFERLRRGVARGKNRLQRLNAMV 78
LS L N P GF L H D K+ T +RLR + R NR+
Sbjct: 15 LSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHF---- 74
Query: 79 LAANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQA 138
Q + + + +GE+LM ++IGTPP AI DTGSDL+WTQC PC C+ Q
Sbjct: 75 --TEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQV 134
Query: 139 TPIFDPKKSSSFSKISCSSELCGALPT-STCS--NNGCEYLYTYGDSSSTEGVLAFETFT 198
P+FDPK SS++ +SCSS C AL ++CS +N C Y +YGD+S T+G +A +T T
Sbjct: 135 DPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLT 194
Query: 199 FGDSSEDQNINFENL-----------FNKIQGEGLVGLGRGPLSLVSQLKEQ---KFAYC 258
G SS+ + + +N+ FNK +G G+VGLG GP+SL+ QL + KF+YC
Sbjct: 195 LG-SSDTRPMQLKNIIIGCGHNNAGTFNK-KGSGIVGLGGGPVSLIKQLGDSIDGKFSYC 254
Query: 259 LTAIDDSK--PSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQL 318
L + K S + G+ A + + + +TPLI SQ +FYYL+L+ ISVG Q+
Sbjct: 255 LVPLTSKKDQTSKINFGTNAIV----SGSGVVSTPLIAKASQETFYYLTLKSISVGSKQI 314
Query: 319 PIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCF 378
S E G +IIDSGTT+T + ++ L++ + ++ GL LC+
Sbjct: 315 QYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCY 374
Query: 379 NLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQN 433
+ + ++VP +T HF GAD++L N + S L+C A S SI+GN+ Q N
Sbjct: 375 SATGD---LKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVAQMN 434
BLAST of Lsi01G019440 vs. ExPASy Swiss-Prot
Match:
Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)
HSP 1 Score: 249.6 bits (636), Expect = 6.5e-65
Identity = 135/365 (36.99%), Postives = 205/365 (56.16%), Query Frame = 0
Query: 81 DQVKAPVVA----GNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 140
+ + PVV+ G+GE+ ++ +GTP K ++DTGSD+ W QC+PC C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204
Query: 141 DPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDSSED 200
+P SS++ ++CS+ C L TS C +N C Y +YGD S T G LA +T TFG+S +
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKI 264
Query: 201 QNI------NFENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLG 260
N+ + E LF GL+GLG G LS+ +Q+K F+YCL D K SSL
Sbjct: 265 NNVALGCGHDNEGLFT--GAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFN 324
Query: 261 SLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELHNDGSGGV 320
S+ T PL+RN +FYY+ L G SVG ++ +P + F++ GSGGV
Sbjct: 325 SV------QLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGV 384
Query: 321 IIDSGTTITYIENTAFTLLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTF 380
I+D GT +T ++ A+ L++ F+ +NL S D C++ + +T V+VP + F
Sbjct: 385 ILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPTVAF 444
Query: 381 HFKGA-DLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSF 433
HF G L+LP +NY+I +G C A +S +SI GN+QQQ + +DL + +
Sbjct: 445 HFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGL 500
BLAST of Lsi01G019440 vs. ExPASy TrEMBL
Match:
A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)
HSP 1 Score: 763.5 bits (1970), Expect = 4.9e-217
Identity = 390/445 (87.64%), Postives = 412/445 (92.58%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
+L TTLFI+T A SS+LSRRALQKPNKLPS+GFRV+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 ILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQ+TPIFDPK+SSSF KISCSSELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMKTTPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITY+EN+AFT LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA QVEVPKLTFHFKGADLELPGENYMIGDS+AGL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. ExPASy TrEMBL
Match:
A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)
HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 389/445 (87.42%), Postives = 410/445 (92.13%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
V TTLFI+T A SS+LS RALQKPNKLPS+GFRV+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 VFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA+VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQATPIFDPK+SSSFSKISC SELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. ExPASy TrEMBL
Match:
A0A5D3BTY9 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00580 PE=3 SV=1)
HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 389/445 (87.42%), Postives = 410/445 (92.13%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
V TTLFI+T A SS+LSRRALQKPNKLPS+GF V+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 VFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA+VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQATPIFDPK+SSSFSKISC SELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. ExPASy TrEMBL
Match:
A0A1S3B573 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 SV=1)
HSP 1 Score: 758.8 bits (1958), Expect = 1.2e-215
Identity = 389/445 (87.42%), Postives = 410/445 (92.13%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
V TTLFI+T A SS+LS RALQKPNKLPS+GFRV+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 VFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA+VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQATPIFDPK+SSSFSKISC SELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. ExPASy TrEMBL
Match:
A0A6J1EPU6 (aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 PE=3 SV=1)
HSP 1 Score: 711.4 bits (1835), Expect = 2.2e-201
Identity = 364/441 (82.54%), Postives = 392/441 (88.89%), Query Frame = 0
Query: 6 TTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGKNRL 65
T LFI TSA SS+LSRRAL +P KLPS+GFRV LNHVDHVKNLTRFERL+RGVARGK RL
Sbjct: 17 TMLFIHTSASSSSLSRRALWQP-KLPSDGFRVSLNHVDHVKNLTRFERLQRGVARGKTRL 76
Query: 66 QRLNAMVLAANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPC 125
RLNAMVLAAN VG +V+APVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQCKPC
Sbjct: 77 HRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPC 136
Query: 126 QQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAF 185
QQCFDQATPIFDPK+SSSFSKISCSSELC ALPTSTCS++ CEY YTYGD SST GVLA
Sbjct: 137 QQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTYGDYSSTHGVLAA 196
Query: 186 ETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAY 245
ETFTFGDSS+DQ + N + F+ QGEGLVGLGRGPLSLVSQLKEQKF+Y
Sbjct: 197 ETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFS--QGEGLVGLGRGPLSLVSQLKEQKFSY 256
Query: 246 CLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLP 305
CLTAIDD+KPSSLL+GSLAN+KPK ++ E+KTTPLIRNPSQPSFYYLSLQGISVG TQLP
Sbjct: 257 CLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQGISVGGTQLP 316
Query: 306 IPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCFN 365
IPK+TFELH+DGSGGVIIDSGTTITYIE AFTLLK EF++QM LPVDDSGT GLDLCFN
Sbjct: 317 IPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDSGTSGLDLCFN 376
Query: 366 LPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQNF 425
LP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSS GMSIFGNLQQQN
Sbjct: 377 LPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMSIFGNLQQQNI 436
Query: 426 MVVHDLQEETLSFLPTQCDSI 436
MVVHDLQEET+SFLPTQC I
Sbjct: 437 MVVHDLQEETVSFLPTQCSEI 454
BLAST of Lsi01G019440 vs. NCBI nr
Match:
XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])
HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 398/444 (89.64%), Postives = 412/444 (92.79%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
VL TTLFIDT A LSRRALQK N+LPSNGF+VKLNHVDHVKNLTRFERLRRGVARGK
Sbjct: 17 VLITTLFIDTLA----LSRRALQKSNELPSNGFKVKLNHVDHVKNLTRFERLRRGVARGK 76
Query: 63 NRLQRLNAMVLAANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQC 122
NRL RLNAMVLAANAAVG+QV+APVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQC
Sbjct: 77 NRLHRLNAMVLAANAAVGEQVQAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQC 136
Query: 123 KPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGV 182
KPCQQCFDQATPIFDPK SSSFSKISCSSELCG LPTSTCS++GCEYLYTYGDSSST+GV
Sbjct: 137 KPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPTSTCSSDGCEYLYTYGDSSSTQGV 196
Query: 183 LAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQK 242
LA ETFTFGDSS+DQ + N + F+ QG GLVGLGRGPLSLVSQLKEQK
Sbjct: 197 LALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFS--QGAGLVGLGRGPLSLVSQLKEQK 256
Query: 243 FAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDT 302
FAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVG T
Sbjct: 257 FAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGST 316
Query: 303 QLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDL 362
QL IPK+TFELH+DGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDL
Sbjct: 317 QLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDL 376
Query: 363 CFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQ 422
CFNLPAEATQVEVPKLTFHF+GADLELPGENYMIGDSR GLICLAIGSSRGMSIFGNLQQ
Sbjct: 377 CFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRTGLICLAIGSSRGMSIFGNLQQ 436
Query: 423 QNFMVVHDLQEETLSFLPTQCDSI 436
QNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 437 QNFMVVHDLQEETLSFLPTQCDSI 454
BLAST of Lsi01G019440 vs. NCBI nr
Match:
XP_011653928.1 (aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical protein Csa_012038 [Cucumis sativus])
HSP 1 Score: 763.5 bits (1970), Expect = 1.0e-216
Identity = 390/445 (87.64%), Postives = 412/445 (92.58%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
+L TTLFI+T A SS+LSRRALQKPNKLPS+GFRV+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 ILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQ+TPIFDPK+SSSF KISCSSELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMKTTPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITY+EN+AFT LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA QVEVPKLTFHFKGADLELPGENYMIGDS+AGL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. NCBI nr
Match:
TYK02448.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])
HSP 1 Score: 758.8 bits (1958), Expect = 2.5e-215
Identity = 389/445 (87.42%), Postives = 410/445 (92.13%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
V TTLFI+T A SS+LSRRALQKPNKLPS+GF V+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 VFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA+VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQATPIFDPK+SSSFSKISC SELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. NCBI nr
Match:
XP_008442220.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])
HSP 1 Score: 758.8 bits (1958), Expect = 2.5e-215
Identity = 389/445 (87.42%), Postives = 410/445 (92.13%), Query Frame = 0
Query: 3 VLSTTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGK 62
V TTLFI+T A SS+LS RALQKPNKLPS+GFRV+L HVDHVKNLTRFERLRRGVARGK
Sbjct: 19 VFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGK 78
Query: 63 NRLQRLNAMVL-AANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 122
NRL RLNAMVL AANA+VGDQVKAPVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQ
Sbjct: 79 NRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQ 138
Query: 123 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEG 182
CKPCQQCFDQATPIFDPK+SSSFSKISC SELCGALPTSTCS++GCEYLYTYGDSSST+G
Sbjct: 139 CKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQG 198
Query: 183 VLAFETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ 242
VLAFETFTFGDS+EDQ N N + F+ QG GLVGLGRGPLSLVSQLKEQ
Sbjct: 199 VLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFS--QGAGLVGLGRGPLSLVSQLKEQ 258
Query: 243 KFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGD 302
KFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPSFYYLSLQGISVG
Sbjct: 259 KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGG 318
Query: 303 TQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLD 362
TQL IPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQMNLPVDDSGTGGLD
Sbjct: 319 TQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLD 378
Query: 363 LCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQ 422
LCFNLPA TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAIGSSRGMSIFGNLQ
Sbjct: 379 LCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQ 438
Query: 423 QQNFMVVHDLQEETLSFLPTQCDSI 436
QQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 439 QQNFMVVHDLQEETLSFLPTQCDSI 461
BLAST of Lsi01G019440 vs. NCBI nr
Match:
XP_022928703.1 (aspartic proteinase nepenthesin-1 [Cucurbita moschata])
HSP 1 Score: 711.4 bits (1835), Expect = 4.6e-201
Identity = 364/441 (82.54%), Postives = 392/441 (88.89%), Query Frame = 0
Query: 6 TTLFIDTSALSSTLSRRALQKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGKNRL 65
T LFI TSA SS+LSRRAL +P KLPS+GFRV LNHVDHVKNLTRFERL+RGVARGK RL
Sbjct: 17 TMLFIHTSASSSSLSRRALWQP-KLPSDGFRVSLNHVDHVKNLTRFERLQRGVARGKTRL 76
Query: 66 QRLNAMVLAANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPC 125
RLNAMVLAAN VG +V+APVVAGNGEFLMKLAIG+PP+SFSAIMDTGSDLIWTQCKPC
Sbjct: 77 HRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPC 136
Query: 126 QQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAF 185
QQCFDQATPIFDPK+SSSFSKISCSSELC ALPTSTCS++ CEY YTYGD SST GVLA
Sbjct: 137 QQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTYGDYSSTHGVLAA 196
Query: 186 ETFTFGDSSEDQ-----------NINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAY 245
ETFTFGDSS+DQ + N + F+ QGEGLVGLGRGPLSLVSQLKEQKF+Y
Sbjct: 197 ETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFS--QGEGLVGLGRGPLSLVSQLKEQKFSY 256
Query: 246 CLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLP 305
CLTAIDD+KPSSLL+GSLAN+KPK ++ E+KTTPLIRNPSQPSFYYLSLQGISVG TQLP
Sbjct: 257 CLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQGISVGGTQLP 316
Query: 306 IPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCFN 365
IPK+TFELH+DGSGGVIIDSGTTITYIE AFTLLK EF++QM LPVDDSGT GLDLCFN
Sbjct: 317 IPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDSGTSGLDLCFN 376
Query: 366 LPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQNF 425
LP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSS GMSIFGNLQQQN
Sbjct: 377 LPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMSIFGNLQQQNI 436
Query: 426 MVVHDLQEETLSFLPTQCDSI 436
MVVHDLQEET+SFLPTQC I
Sbjct: 437 MVVHDLQEETVSFLPTQCSEI 454
BLAST of Lsi01G019440 vs. TAIR 10
Match:
AT2G03200.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 509.6 bits (1311), Expect = 2.5e-144
Identity = 267/447 (59.73%), Postives = 333/447 (74.50%), Query Frame = 0
Query: 8 LFIDTSALSSTLSRRAL---QKPNKLPSNGFRVKLNHVDHVKNLTRFERLRRGVARGKNR 67
L + + +S + SRR+L P LP +GFR+ L HVD KNLT+ ++++RG+ RG +R
Sbjct: 15 LILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFHR 74
Query: 68 LQRLNAMVLAANAAVGD---QVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQ 127
L RL A+ + A A+ D +KAP G+GEFLM+L+IG P +SAI+DTGSDLIWTQ
Sbjct: 75 LNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQ 134
Query: 128 CKPCQQCFDQATPIFDPKKSSSFSKISCSSELCGALPTSTCS--NNGCEYLYTYGDSSST 187
CKPC +CFDQ TPIFDP+KSSS+SK+ CSS LC ALP S C+ + CEYLYTYGD SST
Sbjct: 135 CKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSST 194
Query: 188 EGVLAFETFTFGDSSEDQNINF----ENLFNKI-QGEGLVGLGRGPLSLVSQLKEQKFAY 247
G+LA ETFTF D + I F EN + QG GLVGLGRGPLSL+SQLKE KF+Y
Sbjct: 195 RGLLATETFTFEDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSY 254
Query: 248 CLTAIDDSK-PSSLLLGSLAN-IKPKT----TKDEMKTTPLIRNPSQPSFYYLSLQGISV 307
CLT+I+DS+ SSL +GSLA+ I KT + KT L+RNP QPSFYYL LQGI+V
Sbjct: 255 CLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITV 314
Query: 308 GDTQLPIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGG 367
G +L + KSTFEL DG+GG+IIDSGTTITY+E TAF +LK EF ++M+LPVDDSG+ G
Sbjct: 315 GAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTG 374
Query: 368 LDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGN 427
LDLCF LP A + VPK+ FHFKGADLELPGENYM+ DS G++CLA+GSS GMSIFGN
Sbjct: 375 LDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGMSIFGN 434
Query: 428 LQQQNFMVVHDLQEETLSFLPTQCDSI 436
+QQQNF V+HDL++ET+SF+PT+C +
Sbjct: 435 VQQQNFNVLHDLEKETVSFVPTECGKL 461
BLAST of Lsi01G019440 vs. TAIR 10
Match:
AT5G33340.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 254.2 bits (648), Expect = 1.9e-67
Identity = 162/439 (36.90%), Postives = 235/439 (53.53%), Query Frame = 0
Query: 19 LSRRALQKPNKLPSNGFRVKLNHVDHVKN------LTRFERLRRGVARGKNRLQRLNAMV 78
LS L N P GF L H D K+ T +RLR + R NR+
Sbjct: 15 LSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHF---- 74
Query: 79 LAANAAVGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQA 138
Q + + + +GE+LM ++IGTPP AI DTGSDL+WTQC PC C+ Q
Sbjct: 75 --TEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQV 134
Query: 139 TPIFDPKKSSSFSKISCSSELCGALPT-STCS--NNGCEYLYTYGDSSSTEGVLAFETFT 198
P+FDPK SS++ +SCSS C AL ++CS +N C Y +YGD+S T+G +A +T T
Sbjct: 135 DPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLT 194
Query: 199 FGDSSEDQNINFENL-----------FNKIQGEGLVGLGRGPLSLVSQLKEQ---KFAYC 258
G SS+ + + +N+ FNK +G G+VGLG GP+SL+ QL + KF+YC
Sbjct: 195 LG-SSDTRPMQLKNIIIGCGHNNAGTFNK-KGSGIVGLGGGPVSLIKQLGDSIDGKFSYC 254
Query: 259 LTAIDDSK--PSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQL 318
L + K S + G+ A + + + +TPLI SQ +FYYL+L+ ISVG Q+
Sbjct: 255 LVPLTSKKDQTSKINFGTNAIV----SGSGVVSTPLIAKASQETFYYLTLKSISVGSKQI 314
Query: 319 PIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCF 378
S E G +IIDSGTT+T + ++ L++ + ++ GL LC+
Sbjct: 315 QYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCY 374
Query: 379 NLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQN 433
+ + ++VP +T HF GAD++L N + S L+C A S SI+GN+ Q N
Sbjct: 375 SATGD---LKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVAQMN 434
BLAST of Lsi01G019440 vs. TAIR 10
Match:
AT1G25510.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 157/422 (37.20%), Postives = 221/422 (52.37%), Query Frame = 0
Query: 36 RVKLNHVDH--VKNLTRFERLRRGVARGKNRLQRLNAMVLAANAA-----------VGDQ 95
RV + +H K+LT RL R AR K+ + RL+ + + A
Sbjct: 74 RVSVRGTEHSDYKSLT-LARLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQD 133
Query: 96 VKAPVVA----GNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDP 155
++AP+++ G+GE+ ++ IG P + ++DTGSD+ W QC PC C+ Q PIF+P
Sbjct: 134 IEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEP 193
Query: 156 KKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDSSEDQN 215
SSS+ +SC + C AL S C N C Y +YGD S T G A ET T G S+ QN
Sbjct: 194 SSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIG-STLVQN 253
Query: 216 I------NFENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSL 275
+ + E LF + GL+GLG G L+L SQL F+YCL D S++ G+
Sbjct: 254 VAVGCGHSNEGLF--VGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSASTVDFGT- 313
Query: 276 ANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELHNDGSGGVII 335
+ + PL+RN +FYYL L GISVG L IP+S+FE+ GSGG+II
Sbjct: 314 ------SLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIII 373
Query: 336 DSGTTITYIENTAFTLLKNEFIAQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFK 395
DSGT +T ++ + L++ F+ +G D C+NL A+ T VEVP + FHF
Sbjct: 374 DSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTT-VEVPTVAFHFP 433
Query: 396 GAD-LELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFLPT 433
G L LP +NYMI G CLA ++ ++I GN+QQQ V DL + F
Sbjct: 434 GGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSN 483
BLAST of Lsi01G019440 vs. TAIR 10
Match:
AT3G18490.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 249.6 bits (636), Expect = 4.6e-66
Identity = 135/365 (36.99%), Postives = 205/365 (56.16%), Query Frame = 0
Query: 81 DQVKAPVVA----GNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 140
+ + PVV+ G+GE+ ++ +GTP K ++DTGSD+ W QC+PC C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204
Query: 141 DPKKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDSSED 200
+P SS++ ++CS+ C L TS C +N C Y +YGD S T G LA +T TFG+S +
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKI 264
Query: 201 QNI------NFENLFNKIQGEGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLG 260
N+ + E LF GL+GLG G LS+ +Q+K F+YCL D K SSL
Sbjct: 265 NNVALGCGHDNEGLFT--GAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFN 324
Query: 261 SLANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELHNDGSGGV 320
S+ T PL+RN +FYY+ L G SVG ++ +P + F++ GSGGV
Sbjct: 325 SV------QLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGV 384
Query: 321 IIDSGTTITYIENTAFTLLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTF 380
I+D GT +T ++ A+ L++ F+ +NL S D C++ + +T V+VP + F
Sbjct: 385 ILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPTVAF 444
Query: 381 HFKGA-DLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSF 433
HF G L+LP +NY+I +G C A +S +SI GN+QQQ + +DL + +
Sbjct: 445 HFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGL 500
BLAST of Lsi01G019440 vs. TAIR 10
Match:
AT3G20015.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 242.7 bits (618), Expect = 5.6e-64
Identity = 140/423 (33.10%), Postives = 223/423 (52.72%), Query Frame = 0
Query: 32 SNGFRVKLNHVDHVKNLT-------RFERLRRGVARGKNRLQRLNAMVLAANAA------ 91
S+ + ++L H D ++T R+RR R L+R++ V+ ++ +
Sbjct: 56 SSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVND 115
Query: 92 VGDQVKAPVVAGNGEFLMKLAIGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDP 151
G + + + G+GE+ +++ +G+PP+ ++D+GSD++W QC+PC+ C+ Q+ P+FDP
Sbjct: 116 FGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDP 175
Query: 152 KKSSSFSKISCSSELCGALPTSTCSNNGCEYLYTYGDSSSTEGVLAFETFTFGDS---SE 211
KS S++ +SC S +C + S C + GC Y YGD S T+G LA ET TF + +
Sbjct: 176 AKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNV 235
Query: 212 DQNINFENLFNKIQGEGLVGLGRGPLSLVSQLKEQ---KFAYCLTAIDDSKPSSLLLGSL 271
N I GL+G+G G +S V QL Q F YCL + SL+ G
Sbjct: 236 AMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGR- 295
Query: 272 ANIKPKTTKDEMKTTPLIRNPSQPSFYYLSLQGISVGDTQLPIPKSTFELHNDGSGGVII 331
+ PL+RNP PSFYY+ L+G+ VG ++P+P F+L G GGV++
Sbjct: 296 -----EALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVM 355
Query: 332 DSGTTITYIENTAFTLLKNEFIAQ-MNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHF 391
D+GT +T + A+ ++ F +Q NLP SG D C++L + V VP ++F+F
Sbjct: 356 DTGTAVTRLPTAAYVAFRDGFKSQTANLP-RASGVSIFDTCYDL-SGFVSVRVPTVSFYF 415
Query: 392 -KGADLELPGENYMIGDSRAGLICLAIGSS-RGMSIFGNLQQQNFMVVHDLQEETLSFLP 433
+G L LP N+++ +G C A +S G+SI GN+QQ+ V D + F P
Sbjct: 416 TEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGP 470
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q766C3 | 4.3e-117 | 53.99 | Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... | [more] |
Q766C2 | 8.1e-108 | 48.35 | Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... | [more] |
Q7XV21 | 1.2e-66 | 34.75 | Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2 | [more] |
Q6XBF8 | 2.6e-66 | 36.90 | Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1 | [more] |
Q9LS40 | 6.5e-65 | 36.99 | Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KYT9 | 4.9e-217 | 87.64 | Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G55468... | [more] |
A0A5A7TD10 | 1.2e-215 | 87.42 | Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... | [more] |
A0A5D3BTY9 | 1.2e-215 | 87.42 | Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... | [more] |
A0A1S3B573 | 1.2e-215 | 87.42 | aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 S... | [more] |
A0A6J1EPU6 | 2.2e-201 | 82.54 | aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_038883313.1 | 1.7e-219 | 89.64 | aspartic proteinase nepenthesin-1 [Benincasa hispida] | [more] |
XP_011653928.1 | 1.0e-216 | 87.64 | aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical pro... | [more] |
TYK02448.1 | 2.5e-215 | 87.42 | aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa] | [more] |
XP_008442220.1 | 2.5e-215 | 87.42 | PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspart... | [more] |
XP_022928703.1 | 4.6e-201 | 82.54 | aspartic proteinase nepenthesin-1 [Cucurbita moschata] | [more] |