Lsi01G019290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G019290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAdenylosuccinate synthetase, chloroplastic
Locationchr01: 24269917 .. 24274400 (+)
RNA-Seq ExpressionLsi01G019290
SyntenyLsi01G019290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATCTCTTCTCTTGCTTTTGAGCCCACGCTTTTCTCCGCCGCCAAATCCCCGTCGCCGCGTTGCCGGAGACCCTTTTCTGTTCGTCATCCTGCACTCGTCGTTTGCTCATCTGCTAAGCCCGCCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCAAGGAAGGACGGACTGAAACGAATTGGGGAACTGAGTCAAGTATCGGGAGTCTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTCGAAATCGTCGCTCGCTGTCAGGTAGTCTTCAACATTTGATTCCTTTTCGTTTGCTTTGTTTTCTTTTTGTTTCTTTAGATGATTTTGATGTTTAGGTTTTTGTTGTGTTTCTTGTTTCTTTGTTATTGAATTTCGATTTGTTGTTTGGCTTGCTTACTTGCCTCCCTGTGATTCTTTTTTCATCTCTGATTCTGTACTTTCTCTTTGTTCTTCCTTCGCTTTCCGGTATTTTGACATTTTTGTCTACATTTGTTGAACTTTCGTTTTGTTTATGAAGCATCTGCTTGTAATCTTGCGTTGTTATATATATCGTTTTTGTTTATCGGTGCTGTTTGACGCTACAATATTTGGGTGTCAAGAAACCCGAAGGATATTATATCCTAGATAGGTGGCTACTATGGATTGAACCTATGACCTCTTAGCCCTTTATCAAGGTCATGCCCCCTATTTAGCCAATCCAGGTGGTTATATTTGTAATGGTGAAAGCCAAAGGGCAGCTATGATTTATATCTGTATTTTCACTTCAACTTCAGAAAAGCCTATGAACCATTTTGATTCCATATTGCCTGTTTATATGATATCCTGATGTGAATGCCACTTTAGGTTTCTGGAACTATCTGAAAGTTCAAACTGATTTGCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTCGCTCTTCATCTCATTCCCTCAGGGATCCTAAATGAGGATACTTTATGTGTTATTGGAAACGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAAGAAATTGACCGTCTAGAAGCAAGTGGTGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCCAGTAAAGTGATTCGAAATGGTATCAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTCGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTCGAAGGTGGTCAAGCAACTATGCTTGATATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAGTTTCCTTTAATTCATTTTTTTATTACTTGGTTTAACTGATGAAAGAATTCCAAGTTGTATTCACCTCTTCTCTATTTGAGAATTTGTTTCTGCCATTGTCTCTTTCCTTTATTGTAAATCATGAAAAACTAAGACAGACTTAAAAAGTTTACTCCCTATTATCAGGCACTAATCCTTTCCTGGTTCTCTTTTGGCAATATGTTTGCAAACATACTAATTCATGTGCTCTATGTACTTATTGTCGCTTTAATGACATTATAAAATGATTTTTATGGAAGAAGCCATGGTATCTTAAATTATTATTCAGCCTTGAGAGATTACAATATCATTGGTTTAGGATATTGTAATCTCTATAAAGCCATGAGTGACTGTTTCTTTATTTATATATCGAATATGCATACCCATAATGTTCTCTGTTGATCTTTGCAGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCTACCGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGCCGTCCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTAAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACAAAACTTGACGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTTTCTTATTGGACACCTGATGGAACACCAGTCAAATCGTTTCCTGCAGATCTTAGTCTTCTTGAACAAATAAAGGCAAGCATTATGCATTTTTCCCCCTTTTACCTTGCATTTTGTTAACTTTATACGAAGTTATTGGGATTTGCTTGAATATGGTTGAGTTTTTTATGATTTACGAATTTTTTGAGAAATCAGTTTATAATTCATGCTTAAAGTAGAACTTTATTCTTTTTTTGAAAGGGAGGAATAACTTCTGATTGGGAGTTTTTATTTGAGATAGGAAAGAAAGCTTTTCACCAAAGTTGATCCAATTGAATCATAGGTTACATATATGGTCTGGAATTTTGGTAAGGGTCAGAAACTATGTATATTAGATCATAGGTGGATTTGGGGAAAAAATCTAGTACTACCGTAGTACATACTACCTCTATTCATTATTTAACACGTCATTAAATGATTAATTAATTAATTATTCATTTAATTCGTGGACCCCACTTAATTCTTAAATGCTACTTTTGAAGCAAGCAATGAATGGATGTAATATAGAGAATTTTCCATACTAGCGTAGTACTAGAAATTTTCCCGTGGATTTGTGGTTTTCTCAATGGAAAATCTAGATGGTTGATTTGTGCTTTAGACAATTTGATCATATGTCTACATACGTAGTTTGTTAGATGGGGGTGTATTGAGTAGCCTTGATGATAAAGGTGTTTCAAATGGTGACTTTCATGAATTCTTCAGAAAAAGAAATAAACAAAAATATTAGAATAGAAGATAAATAAAAGACTGTACTCTGGAGAAGGCCTTTCCCATGTGCACTCTTCAGTTAAAAATTTGGGGAGCATTGTGCTGCAAGATTTCGAACTTGGGTAGTTGATGTCTCCATAAAGAACAATATTACTCAATTATTTATGCTTGACACGTGGCTTTAGAGGACGAGCCAAAAACCTTATAGGTTGATTTCATTCATTCTTGTGGTAGACTTTTCAAAAATCAGCAAAAAGTTTGTCTAGTGTTTCAAAAGAGAAAAAGTCTAAAATGCATTTAAGTTGTGTTTGAATGTGTTCTCACTTGTTCTTTCATTGTTGCTTCATACAGTGTTGGGATTGGTTGGGATTATGTACTACTAAGATTTTTGACGTTTTGGGTATTTGATTGTCTTGATAAAAAAAAACTCATGGTTGCTCTCTCTCTTCTCCTCTATTTTTTTTTCCGTTCCTCCTTTCTGCATATCTATTGAACTCATGGTTGTTTTCGAATGTACTTTAGAAAACAATATTATAAAAGGAAAAGGAAATGTTTTAAGTTTCGAGTAAAAAATTTGTCTACAGTCACTGTAGGTTATAGTTAAGACATTATGACATTAACCAAGGTTAGAAGCTTGAATCTCCACTCCCACGTATTGTTGAACTAGACGAAAGTTCTAATTAAAAAATTGAATTTTTTAATATTTTCTAAATTTTTTTTGATGTTGTCCATGCTTCTTAGCATTTTTTGCAACGGTATCCTATATTACTTGCCAATCATTTTCATGCCTTTTCTGTTTCCAAGTAATCAACTAAATTAGTAGTTGAAATGTTCTTAAATTTCAAAGCTCTTCTACTCCTAAATCCAGAATCTTAACAACACTTTTTTTTTTTTTTTTTTTTTTTTCNGAAACTAAGATTCTGGAAAACCTAGAAAAAGAAGAAGAGAAAGTTAAGATTCTGAAAACCTAGAAAAAGAAGAAGAAGAAGAGAAAAAGATGAAGAAGAAAATTAAGAAGAAGATGAAGATGATGACGACGATAGGAGATTTAGAAGAAAAGGCCAGAAGACGATGAAAAAGAAAATGTACCTAGATGAAATCGACCGATGAATATGCACACGATGAAGATGGATGAAAGATCACTGAATGATCAAAATAGAAATCGGCGCGCGAATTTCAAATGGAAAAGGGTAGGTTGAGAATTTTAGAAAATTGAATCCACTACCGTATTTTTTTTTTTTTTTTTTTTTTTTTTTCCATTTGTTTTTCCTGTTTGCAGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAACTTGTGGGCATACCCGTCCATTACATTGGTGTCGGTCCGGGCCGTGATGCACTATTATACAAATGA

mRNA sequence

ATGAGCATCTCTTCTCTTGCTTTTGAGCCCACGCTTTTCTCCGCCGCCAAATCCCCGTCGCCGCGTTGCCGGAGACCCTTTTCTGTTCGTCATCCTGCACTCGTCGTTTGCTCATCTGCTAAGCCCGCCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCAAGGAAGGACGGACTGAAACGAATTGGGGAACTGAGTCAAGTATCGGGAGTCTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTCGAAATCGTCGCTCGCTGTCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTCGCTCTTCATCTCATTCCCTCAGGGATCCTAAATGAGGATACTTTATGTGTTATTGGAAACGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAAGAAATTGACCGTCTAGAAGCAAGTGGTGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCCAGTAAAGTGATTCGAAATGGTATCAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTCGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTCGAAGGTGGTCAAGCAACTATGCTTGATATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCTACCGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGCCGTCCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTAAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACAAAACTTGACGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAACTTGTGGGCATACCCGTCCATTACATTGGTGTCGGTCCGGGCCGTGATGCACTATTATACAAATGA

Coding sequence (CDS)

ATGAGCATCTCTTCTCTTGCTTTTGAGCCCACGCTTTTCTCCGCCGCCAAATCCCCGTCGCCGCGTTGCCGGAGACCCTTTTCTGTTCGTCATCCTGCACTCGTCGTTTGCTCATCTGCTAAGCCCGCCGCGGCTGTTAGTTCTTCTGTCAGTGTGCCGGAGTCGGCAAGGAAGGACGGACTGAAACGAATTGGGGAACTGAGTCAAGTATCGGGAGTCTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGACATCTTGGCCCAGCACTTCGAAATCGTCGCTCGCTGTCAGGGTGGGGCTAATGCTGGGCACACAATATATAATGCAGAAGGGAAGAAGTTCGCTCTTCATCTCATTCCCTCAGGGATCCTAAATGAGGATACTTTATGTGTTATTGGAAACGGAGTTGTGGTGCACGTGCCTGGTTTCTTTGAAGAAATTGACCGTCTAGAAGCAAGTGGTGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTCCATCAGGAGGTGGATGGTCTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCCAGTAAAGTGATTCGAAATGGTATCAGAGTGGGTGATTTGAGGCACTTGGACACTCTTCCTCAGAAGCTGGACCTTCTACTCTCGGATGCTGCTTCGAGATTTAAGGATTTTAAATATACCCCAGAAATTCTGAAGGAAGAAGTCGAAAAGTACAAAAGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGTATTTCATTAATGAAGCCATCTCTGCAAATAAGAAGATTTTGGTCGAAGGTGGTCAAGCAACTATGCTTGATATTGACTTTGGAACTTATCCTTTTGTAACTTCCTCCAGCCCTTCGGCAGGTGGAATTTGCACTGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAAGGCATACACAACTAGAGTTGGTTCTGGCCCTTTTCCTACCGAGATCTTGGGAAAGGGAGGTGACCTACTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGCCGTCCTCGTCGTTGTGGTTGGCTTGATATAGTTGCACTAAAATATTGCTGTCAAATTAATGGCTTCTCTTCTCTGAATCTTACAAAACTTGACGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCGAAAGAATATGTATCAAGGATTGAAGAACTTGTGGGCATACCCGTCCATTACATTGGTGTCGGTCCGGGCCGTGATGCACTATTATACAAATGA

Protein sequence

MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK
Homology
BLAST of Lsi01G019290 vs. ExPASy Swiss-Prot
Match: Q96529 (Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PURA PE=1 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.1e-216
Identity = 376/493 (76.27%), Postives = 419/493 (84.99%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHP-ALVVCSSAKPAAAVSSSVSVPESARKD 60
           MS+SSL  +     A   P  R   P  + HP + V CS+ +PA + S SV+  +SA  +
Sbjct: 1   MSLSSLTLDSNPRFAVGGPYHRRYPP--LHHPRSFVSCSAKRPAVSASLSVAA-DSAATE 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKFA
Sbjct: 61  SLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHL+PSGILNEDT CVIGNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           F+ FKYTPE+L+EEVE YKRYA+RLEP+ITDTV+FIN++IS  KK+LVEGGQATMLDIDF
Sbjct: 241 FQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR 
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLRL 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALK+ CQINGF+SLNLTKLDVLS+L+EIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYE +PGW  DISS+R+YSDLPKAA++YV RIEELVG+P+HY
Sbjct: 421 TPVKSFPGDLRLLEELHVEYEVLPGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHY 480

BLAST of Lsi01G019290 vs. ExPASy Swiss-Prot
Match: A9XLE1 (Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=PURA1 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 3.3e-216
Identity = 364/463 (78.62%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 30  RHPALVVCSSAKPAAAVSSSVSVPESARKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDI 89
           ++P+++VCS+ KP A+V     V ES    GL RI  LSQVSGVLGCQWGDEGKGKLVDI
Sbjct: 50  KNPSIIVCSTTKPLASVVDRHGVSES----GLSRIESLSQVSGVLGCQWGDEGKGKLVDI 109

Query: 90  LAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFE 149
           LA+HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE+T+CVIGNGVVVH+PG F+
Sbjct: 110 LAKHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETVCVIGNGVVVHLPGLFK 169

Query: 150 EIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKV 209
           EID LE++GVSC+GRILVSDRAHLLFDFHQE+DGLREAELAKSFIGTT+RGIGPCYSSKV
Sbjct: 170 EIDGLESNGVSCQGRILVSDRAHLLFDFHQEIDGLREAELAKSFIGTTKRGIGPCYSSKV 229

Query: 210 IRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFIT 269
           IRNGIRV DLRH+DT PQKLD LLSDAA+RF  FKY PE+L+EEVE+YK++AERLEPFIT
Sbjct: 230 IRNGIRVSDLRHMDTFPQKLDRLLSDAAARFPGFKYGPEMLREEVERYKKFAERLEPFIT 289

Query: 270 DTVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDL 329
           DTV+F+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+VVGDL
Sbjct: 290 DTVHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL 349

Query: 330 IGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQIN 389
           +GVVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL+YCCQIN
Sbjct: 350 VGVVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRYCCQIN 409

Query: 390 GFSSLNLTKLDVLSELSEIQLG-------------------------VEYETMPGWMCDI 449
           GF+SLNLTKLDVLS+LSEIQLG                         VEYE + GW  DI
Sbjct: 410 GFASLNLTKLDVLSDLSEIQLGVTYRHPDGSILNSFPSDLRLLEQLKVEYEVLRGWQSDI 469

Query: 450 SSIRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 468
           SSIR YSDLPK+A+EYV RIEELVG+P+HYIG+GPGRDAL+YK
Sbjct: 470 SSIRKYSDLPKSAREYVERIEELVGVPIHYIGIGPGRDALIYK 508

BLAST of Lsi01G019290 vs. ExPASy Swiss-Prot
Match: B9IJ21 (Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PURA PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 3.3e-216
Identity = 379/495 (76.57%), Postives = 417/495 (84.24%), Query Frame = 0

Query: 1   MSISSLAFEPT---LFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESAR 60
           M++SSL  E      + AA  P+     P   R+   V CSS KP+A  SSS+SV ES  
Sbjct: 1   MNLSSLRLESNPRWSYHAAHFPAHHGLNPSFRRN--FVSCSSIKPSA--SSSLSVAESFT 60

Query: 61  KDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKK 120
           +D   RI  LSQVSGVLG QWGDEGKGKLVDILA+HF+IVARCQGGANAGHTIYN+EGKK
Sbjct: 61  RDSASRIESLSQVSGVLGSQWGDEGKGKLVDILAEHFDIVARCQGGANAGHTIYNSEGKK 120

Query: 121 FALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDF 180
           FALHL+PSGILNEDTLCVIGNGVVVH+PG F+EID LEA+GVSC GRILVSDRAHLLFDF
Sbjct: 121 FALHLVPSGILNEDTLCVIGNGVVVHLPGLFKEIDGLEANGVSCTGRILVSDRAHLLFDF 180

Query: 181 HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA 240
           HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRV DLRH+DT PQKLD LLSD A
Sbjct: 181 HQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVCDLRHMDTFPQKLDALLSDVA 240

Query: 241 SRFKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDI 300
           SRF+ FKY PE+LKEEVE+YKR+AERLEPFITDTV+F+NE+I+  KKILVEGGQATMLDI
Sbjct: 241 SRFESFKYGPEMLKEEVERYKRFAERLEPFITDTVHFMNESIAKKKKILVEGGQATMLDI 300

Query: 301 DFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLL 360
           DFGTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYT+RVGSGPFPTEILG+GGDLL
Sbjct: 301 DFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTSRVGSGPFPTEILGQGGDLL 360

Query: 361 RFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG------ 420
           RFAGQEFGTTTGRPRRCGWLD+VALKY CQINGFSSLNLTKLDVLSE SEIQ+G      
Sbjct: 361 RFAGQEFGTTTGRPRRCGWLDVVALKYVCQINGFSSLNLTKLDVLSEFSEIQIGVSYKQI 420

Query: 421 -------------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPV 468
                              V+YE +PGW  DISSIR Y+DLPKAA++YV RIEELVG+P+
Sbjct: 421 DGTPVESFPGDLCLLEQLKVDYEVLPGWKSDISSIRKYADLPKAAQQYVERIEELVGVPI 480

BLAST of Lsi01G019290 vs. ExPASy Swiss-Prot
Match: Q6T7E8 (Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA PE=2 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 3.7e-215
Identity = 366/461 (79.39%), Postives = 406/461 (88.07%), Query Frame = 0

Query: 32  PALVVCSSAKPAAAVSSSVSVPESARKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDILA 91
           P+++VCS+ KP A+V     V +S    GL RI  LSQVSGVLGCQWGDEGKGKLVDILA
Sbjct: 50  PSIIVCST-KPLASVVEHQGVNDS----GLTRIESLSQVSGVLGCQWGDEGKGKLVDILA 109

Query: 92  QHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEEI 151
           +HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE+T+CVIGNGVVVH+PG F+EI
Sbjct: 110 KHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETICVIGNGVVVHLPGLFKEI 169

Query: 152 DRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIR 211
           D LE++GVSC+GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTT+RGIGPCYSSKVIR
Sbjct: 170 DNLESNGVSCQGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTKRGIGPCYSSKVIR 229

Query: 212 NGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITDT 271
           NGIRV DLRH+DT PQ+LDLLLSDAASRF+ F Y  ++LKEEVE+YK++AERLEPFITDT
Sbjct: 230 NGIRVSDLRHMDTFPQQLDLLLSDAASRFQGFNYGRDMLKEEVERYKKFAERLEPFITDT 289

Query: 272 VYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIG 331
           V+F+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+VVGDL+G
Sbjct: 290 VHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLVG 349

Query: 332 VVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQINGF 391
           VVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL++CCQINGF
Sbjct: 350 VVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRFCCQINGF 409

Query: 392 SSLNLTKLDVLSELSEIQLG-------------------------VEYETMPGWMCDISS 451
           +SLNLTKLDVLS+LSEIQLG                         VEYE MPGW  DISS
Sbjct: 410 ASLNLTKLDVLSDLSEIQLGVTYRHPDGSTLNSFPSDIRLLEQIKVEYEVMPGWQSDISS 469

Query: 452 IRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 468
           +R YSDLPKAA+EYV RIEELVG+PVHYIGVGPGRDAL+YK
Sbjct: 470 VRKYSDLPKAAREYVERIEELVGVPVHYIGVGPGRDALIYK 505

BLAST of Lsi01G019290 vs. ExPASy Swiss-Prot
Match: B9SL58 (Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA2 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 4.8e-215
Identity = 369/462 (79.87%), Postives = 396/462 (85.71%), Query Frame = 0

Query: 31  HPALVVCSSAKPAAAVSSSVSVPESARKDGLKRIGELSQVSGVLGCQWGDEGKGKLVDIL 90
           H   VVCS   P  +  S     +SA  D L RI  LSQVSGVLGCQWGDEGKGKLVDIL
Sbjct: 29  HRNFVVCSIKSP--STPSLNLATDSASTDSLSRINSLSQVSGVLGCQWGDEGKGKLVDIL 88

Query: 91  AQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEDTLCVIGNGVVVHVPGFFEE 150
           AQHF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNEDTLCVIGNGVVVH+PG F+E
Sbjct: 89  AQHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEDTLCVIGNGVVVHLPGLFKE 148

Query: 151 IDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVI 210
           ID LE+SGVSC GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCY+SK I
Sbjct: 149 IDGLESSGVSCTGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYASKAI 208

Query: 211 RNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFKYTPEILKEEVEKYKRYAERLEPFITD 270
           RNGIRV DLRH+DT PQKLD+LLSDAASRF+ FKY PE+L+EEVEKYKRYAERLEPFI D
Sbjct: 209 RNGIRVSDLRHMDTFPQKLDILLSDAASRFQGFKYGPEMLREEVEKYKRYAERLEPFIAD 268

Query: 271 TVYFINEAISANKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLI 330
           TV+ +NE+I   KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+ VGDLI
Sbjct: 269 TVHVMNESIEQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRAVGDLI 328

Query: 331 GVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING 390
           GVVKAYTTRVGSGPFPTEILG+GGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING
Sbjct: 329 GVVKAYTTRVGSGPFPTEILGQGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING 388

Query: 391 FSSLNLTKLDVLSELSEIQLG-------------------------VEYETMPGWMCDIS 450
           FSSLNLTKLDVLS+L EIQLG                         VEYE +PGW  DIS
Sbjct: 389 FSSLNLTKLDVLSDLPEIQLGVAYKHADGTPFKSFPADLRLLEQLKVEYEVLPGWKSDIS 448

Query: 451 SIRDYSDLPKAAKEYVSRIEELVGIPVHYIGVGPGRDALLYK 468
           SIR+Y+D PKAA++YV RIEELVG+PVHYIGVGPGRDAL+YK
Sbjct: 449 SIRNYADFPKAAQQYVERIEELVGVPVHYIGVGPGRDALIYK 488

BLAST of Lsi01G019290 vs. ExPASy TrEMBL
Match: A0A6J1ELC2 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435468 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.8e-257
Identity = 452/492 (91.87%), Postives = 457/492 (92.89%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           MSISSLAF+PTLFSAAK  SP CRRPFS RHPALVVCSSAKPAAAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. ExPASy TrEMBL
Match: A0A0A0L1E1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.4e-257
Identity = 450/493 (91.28%), Postives = 457/493 (92.70%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAK-SPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKD 60
           M+ISSLAFEPTLFSAA  SPS  CRRPFS RHP L+VCSSAK  AAVSSSVSVPES RKD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFSGRHPPLIVCSSAKTVAAVSSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+LKEEVE YKRYAERLEPFITDTVYFINEAISANK+ILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLKEEVENYKRYAERLEPFITDTVYFINEAISANKRILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYETMPGWMCDISSIRDYSDLPKAAKEY+SRIEELVG+PVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGLPVHY 480

BLAST of Lsi01G019290 vs. ExPASy TrEMBL
Match: A0A6J1HXQ5 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467197 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.0e-257
Identity = 450/492 (91.46%), Postives = 456/492 (92.68%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           MSISSLAF+PTLFSAAK PSP CRRPFS RHPAL+VCSSAKPAAAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF Y+PEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. ExPASy TrEMBL
Match: A0A5A7UY81 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=PURA PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.6e-256
Identity = 449/493 (91.08%), Postives = 455/493 (92.29%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAK-SPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKD 60
           M+ISSLAFEPTLFSAA  SPS  CRRPF  R+P L VCSSAK  AAV SSVSVPES RKD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYETMPGWMCDISSIRDYSDLPKAAKEY+SRIEELVGIPVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHY 480

BLAST of Lsi01G019290 vs. ExPASy TrEMBL
Match: A0A1S3B4T1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486156 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.6e-256
Identity = 449/493 (91.08%), Postives = 455/493 (92.29%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAK-SPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKD 60
           M+ISSLAFEPTLFSAA  SPS  CRRPF  R+P L VCSSAK  AAV SSVSVPES RKD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYETMPGWMCDISSIRDYSDLPKAAKEY+SRIEELVGIPVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHY 480

BLAST of Lsi01G019290 vs. NCBI nr
Match: XP_038882112.1 (LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispida])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 454/492 (92.28%), Postives = 459/492 (93.29%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           M+ISSLAFEPTLFSAA  PSP CRRP S RHPALVVCSS KPAAAVSSSVSVP+SARKDG
Sbjct: 1   MNISSLAFEPTLFSAANXPSPACRRPXSARHPALVVCSSTKPAAAVSSSVSVPDSARKDG 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQV GVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVXGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG
Sbjct: 241 KDFNYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQ+G         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQVGVSYWAPDGT 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAK+Y+SRIEELVGIPVHYI
Sbjct: 421 PVKSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKDYISRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. NCBI nr
Match: XP_022928609.1 (adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 897.9 bits (2319), Expect = 3.7e-257
Identity = 452/492 (91.87%), Postives = 457/492 (92.89%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           MSISSLAF+PTLFSAAK  SP CRRPFS RHPALVVCSSAKPAAAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. NCBI nr
Match: KAG6603253.1 (Adenylosuccinate synthetase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037386.1 Adenylosuccinate synthetase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.9 bits (2319), Expect = 3.7e-257
Identity = 452/492 (91.87%), Postives = 457/492 (92.89%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           MSISSLAF+PTLFSAAK  SP CRRPFS RHPALVVCSSAKPAAAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGM 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. NCBI nr
Match: XP_004144664.1 (adenylosuccinate synthetase, chloroplastic [Cucumis sativus] >KGN54874.1 hypothetical protein Csa_012933 [Cucumis sativus])

HSP 1 Score: 897.5 bits (2318), Expect = 4.9e-257
Identity = 450/493 (91.28%), Postives = 457/493 (92.70%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAK-SPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKD 60
           M+ISSLAFEPTLFSAA  SPS  CRRPFS RHP L+VCSSAK  AAVSSSVSVPES RKD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFSGRHPPLIVCSSAKTVAAVSSSVSVPESGRKD 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
           GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           FKDF YTPE+LKEEVE YKRYAERLEPFITDTVYFINEAISANK+ILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLKEEVENYKRYAERLEPFITDTVYFINEAISANKRILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYETMPGWMCDISSIRDYSDLPKAAKEY+SRIEELVG+PVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGLPVHY 480

BLAST of Lsi01G019290 vs. NCBI nr
Match: XP_022967784.1 (adenylosuccinate synthetase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 8.3e-257
Identity = 450/492 (91.46%), Postives = 456/492 (92.68%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHPALVVCSSAKPAAAVSSSVSVPESARKDG 60
           MSISSLAF+PTLFSAAK PSP CRRPFS RHPAL+VCSSAKPAAAVSSSVS PESA KD 
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300
           KDF Y+PEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG--------- 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLG         
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 ----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 468
                           VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 480

BLAST of Lsi01G019290 vs. TAIR 10
Match: AT3G57610.1 (adenylosuccinate synthase )

HSP 1 Score: 753.8 bits (1945), Expect = 8.1e-218
Identity = 376/493 (76.27%), Postives = 419/493 (84.99%), Query Frame = 0

Query: 1   MSISSLAFEPTLFSAAKSPSPRCRRPFSVRHP-ALVVCSSAKPAAAVSSSVSVPESARKD 60
           MS+SSL  +     A   P  R   P  + HP + V CS+ +PA + S SV+  +SA  +
Sbjct: 1   MSLSSLTLDSNPRFAVGGPYHRRYPP--LHHPRSFVSCSAKRPAVSASLSVAA-DSAATE 60

Query: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKFA
Sbjct: 61  SLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKFA 120

Query: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHL+PSGILNEDT CVIGNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFKYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300
           F+ FKYTPE+L+EEVE YKRYA+RLEP+ITDTV+FIN++IS  KK+LVEGGQATMLDIDF
Sbjct: 241 FQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR 
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLRL 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLG-------- 420
           AGQEFGTTTGRPRRCGWLDIVALK+ CQINGF+SLNLTKLDVLS+L+EIQLG        
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSDG 420

Query: 421 -----------------VEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHY 468
                            VEYE +PGW  DISS+R+YSDLPKAA++YV RIEELVG+P+HY
Sbjct: 421 TPVKSFPGDLRLLEELHVEYEVLPGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHY 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q965291.1e-21676.27Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU... [more]
A9XLE13.3e-21678.62Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=P... [more]
B9IJ213.3e-21676.57Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PUR... [more]
Q6T7E83.7e-21579.39Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA ... [more]
B9SL584.8e-21579.87Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA... [more]
Match NameE-valueIdentityDescription
A0A6J1ELC21.8e-25791.87Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0L1E12.4e-25791.28Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE... [more]
A0A6J1HXQ54.0e-25791.46Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7UY817.6e-25691.08Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B4T17.6e-25691.08Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034861... [more]
Match NameE-valueIdentityDescription
XP_038882112.12.1e-26092.28LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispi... [more]
XP_022928609.13.7e-25791.87adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata][more]
KAG6603253.13.7e-25791.87Adenylosuccinate synthetase, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_004144664.14.9e-25791.28adenylosuccinate synthetase, chloroplastic [Cucumis sativus] >KGN54874.1 hypothe... [more]
XP_022967784.18.3e-25791.46adenylosuccinate synthetase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G57610.18.1e-21876.27adenylosuccinate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001114Adenylosuccinate synthetaseSMARTSM00788adenylsucc_syntcoord: 70..466
e-value: 2.6E-237
score: 804.3
IPR001114Adenylosuccinate synthetaseTIGRFAMTIGR00184TIGR00184coord: 73..463
e-value: 2.1E-142
score: 472.8
IPR001114Adenylosuccinate synthetasePFAMPF00709Adenylsucc_syntcoord: 73..415
e-value: 3.8E-145
score: 484.0
IPR001114Adenylosuccinate synthetasePANTHERPTHR11846ADENYLOSUCCINATE SYNTHETASEcoord: 411..467
IPR001114Adenylosuccinate synthetasePANTHERPTHR11846ADENYLOSUCCINATE SYNTHETASEcoord: 30..414
IPR001114Adenylosuccinate synthetaseHAMAPMF_00011Adenylosucc_synthcoord: 1..467
score: 163.451385
IPR001114Adenylosuccinate synthetaseCDDcd03108AdSScoord: 69..466
e-value: 0.0
score: 552.105
IPR042111Adenylosuccinate synthetase, domain 3GENE3D3.90.170.10Adenylosuccinate Synthetase, subunit A, domain 3coord: 333..412
e-value: 5.5E-38
score: 132.0
coord: 413..467
e-value: 4.0E-18
score: 67.4
IPR042109Adenylosuccinate synthetase, domain 1GENE3D3.40.440.10Adenylosuccinate Synthetase, subunit A, domain 1coord: 73..332
e-value: 8.2E-112
score: 375.1
IPR042110Adenylosuccinate synthetase, domain 2GENE3D1.10.300.10Adenylosuccinate Synthetase, subunit A, domain 2coord: 169..267
e-value: 8.2E-112
score: 375.1
NoneNo IPR availablePANTHERPTHR11846:SF14ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTICcoord: 411..467
NoneNo IPR availablePANTHERPTHR11846:SF14ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTICcoord: 30..414
IPR033128Adenylosuccinate synthase, active sitePROSITEPS00513ADENYLOSUCCIN_SYN_2coord: 200..211
IPR018220Adenylosuccinate synthase, GTP-binding sitePROSITEPS01266ADENYLOSUCCIN_SYN_1coord: 77..84
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 69..465

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G019290.1Lsi01G019290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044208 'de novo' AMP biosynthetic process
biological_process GO:0046040 IMP metabolic process
biological_process GO:0006164 purine nucleotide biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004019 adenylosuccinate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0000287 magnesium ion binding