Lsi01G018210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G018210
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease H
Location	chr01: 18486995 .. 18488904 (-)
RNA-Seq Expression	Lsi01G018210
Synteny	Lsi01G018210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAAGCAGAGTTAACCATGGTAACACAAGTCAGTTGCCGCATCCAAACTCTGGAATTCCTCATTATCCACATAATTATTATGCACCACCTCATCCATATTATCAACCTTACGTAAATCATGCAGCAGTCCAATATTATCCCCCTCCTCCTCAAAATCAACGCTCTTATGTGAATCAGGGGTACCAGCCTCAAGGTCAACAAAATTCTTATGCTCGAGGACAAGATAATAATAAAGGGGGTCGTAAACAAGTGCATTTTGATCCGATTCCAATGACTTACACAGAGCTTCTGCCACGACTATTTCAGAATAATCAACTAGCACCTGTACCAATAGAACCACTGCAACCTCCTTATCCAAAGTGGTATGACCCAAATGCCCGTTGTGATTATCATGCAGGAGTCATTGGGCATTCAACTGAGAATTGTACTGCACTTAAGTACAGGGTACAGGCATTGATCAAGGCGGGATGGCTGAATTTTAAGAAAGACAATGGACCTGACGTAAATAATAACCCTCTTCCAAACCACCAAAATCCACAAGTAAATGTTGTAGATGTGGCTGAGGTTGGTTTAAGAGATGTCGTGAGAATGATAACATCCAAAGAAGAGCTTTTTCGAATTTTGTTTAACAACAGATTGATCGAACAAGGGAATTTGCAAGATGATGTCCTCGTTAGCCAATACGATGACAGTTTATTATGTTCATATCATGCTGGGGCAAAAGGTCACTCTATTGACCAGTACCCTTATTTCTCCCAGAAGGTACAAGAATTATTAAATTCTCATTTCCTAACAGTCTCACAGAAGGTAGTTGGAGAATCAGAGCGTGAGCATGAAATAGATGCTGCAGAAGAGTTCTCAACAGGTGAATGCTCGAAGGTTTCTCTGGAACGAAAGCCATAACAATCTTTTATAAGGAAAAACCCAGTACTACCACTTCCAAACCAAAACCAATTATCATACAGGTTCCATCTCCATTTGAGTATAAAAGTTCAAAAGCAGTACCATGGAATTATGAATATAAGGTACTTGTTGAGTCTGTACCAGTTCCTATAGACAATATTAATGAAATTGGAGGTACAACACGAAGTGGGAAGTGTTATACGCCAGAAGCTTTATTGAAGTACAATAGCAAGGAAAAAGGGAAAGCCAAGCTTAGTGATGTCATTGATTGTAGGATAGAAGACCCACTGTTGGTGAGAAATTAAGAAGTAAAGGAGCCAGCATCTGAAGATGACATCCAAGAGTTTTTAAAACTTATAAAACAAAGTGATTATAAAGTAATTGAACAGTTGGGCAAAACGCCTGCTAGAATCTCTATTTTGGCCTTATTGTTATCTTCAGACCTGCATCGCAAAACTCTAATGAATATCCTGAACTAGACTTATGTTCCATCAGACAATTTAGACAACATCGTTGGCAGTATAATAGCATCAAGTTCCATAACTTTCACTGATGATGAGATACCGCCGGAAGGCATGGGTCACACAAAGGCCTTGCATATTACAGTTAAGTGCAAGAAATTTGCTGTGGCCAAAGTTTTAGTTGACAATGGCTCTTCTTTGAACATACTGCCTATGTCTACATTGGAAAAATTGTCTGTTGATATGTCATACCTCCAATCAAGCACTATGATAGTGAGGGCCTTTGATGGATCGCGCAGTGAAGTGGTTGGGGACATAGAAATCCCTATTCAAATAGGCCCTTGCATCTTTGACATAACTTTCCAAGTCATGAACATCAACTTAGCCTATAGTTTCCTGTTAGGTCGTCCATGGATTCATTCAGCAGGAGTAGTCCCTTCGTCATTGCACCAATGCCTTAAATTTGTAGTCAATCGCAAGCTAGTGATCGTATCAGGACAAGAGGATATTTTTGTGTCAAGCCATCCTCAATGCCATATATAG

mRNA sequence

ATGATAAGCAGAGTTAACCATGGTAACACAAGTCAGTTGCCGCATCCAAACTCTGGAATTCCTCATTATCCACATAATTATTATGCACCACCTCATCCATATTATCAACCTTACGTAAATCATGCAGCAGTCCAATATTATCCCCCTCCTCCTCAAAATCAACGCTCTTATGTGAATCAGGGGTACCAGCCTCAAGATGTGGCTGAGGTTGGTTTAAGAGATGTCGTGAGAATGATAACATCCAAAGAAGAGCTTTTTCGAATTTTGTTTAACAACAGATTGATCGAACAAGGGAATTTGCAAGATGATGTCCTCGTTAGCCAATACGATGACAGTTTATTATGTTCATATCATGCTGGGGCAAAAGGTCACTCTATTGACCAGTACCCTTATTTCTCCCAGAAGGTACAAGAATTATTAAATTCTCATTTCCTAACAGTCTCACAGAAGGTAGTTGGAGAATCAGAGCGTGAGCATGAAATAGATGCTGCAGAAGAGTTCTCAACAGGTGAATGCTCGAAGGTTCCATCTCCATTTGAGTATAAAAGTTCAAAAGCAGTACCATGGAATTATGAATATAAGGTACTTGTTGAGTCTGTACCAGTTCCTATAGACAATATTAATGAAATTGGAGGTACAACACGAAGTGGGAAGTGTTATACGCCAGAAGCTTTATTGAAGTACAATAGCAAGGAAAAAGGGAAAGCCAAGCTTAGTGATGTCATTGATTGTAGGATAGAAGACCCACTGTTGACTTATGTTCCATCAGACAATTTAGACAACATCGTTGGCAGTATAATAGCATCAAGTTCCATAACTTTCACTGATGATGAGATACCGCCGGAAGGCATGGGTCACACAAAGGCCTTGCATATTACAGTTAAGTGCAAGAAATTTGCTGTGGCCAAAGTTTTAGTTGACAATGGCTCTTCTTTGAACATACTGCCTATGTCTACATTGGAAAAATTGTCTGTTGATATGTCATACCTCCAATCAAGCACTATGATAGTGAGGGCCTTTGATGGATCGCGCAGTGAAGTGGTTGGGGACATAGAAATCCCTATTCAAATAGGCCCTTGCATCTTTGACATAACTTTCCAAGTCATGAACATCAACTTAGCCTATAGTTTCCTGTTAGGTCGTCCATGGATTCATTCAGCAGGAGTAGTCCCTTCGTCATTGCACCAATGCCTTAAATTTGTAGTCAATCGCAAGCTAGTGATCGTATCAGGACAAGAGGATATTTTTGTGTCAAGCCATCCTCAATGCCATATATAG

Coding sequence (CDS)

ATGATAAGCAGAGTTAACCATGGTAACACAAGTCAGTTGCCGCATCCAAACTCTGGAATTCCTCATTATCCACATAATTATTATGCACCACCTCATCCATATTATCAACCTTACGTAAATCATGCAGCAGTCCAATATTATCCCCCTCCTCCTCAAAATCAACGCTCTTATGTGAATCAGGGGTACCAGCCTCAAGATGTGGCTGAGGTTGGTTTAAGAGATGTCGTGAGAATGATAACATCCAAAGAAGAGCTTTTTCGAATTTTGTTTAACAACAGATTGATCGAACAAGGGAATTTGCAAGATGATGTCCTCGTTAGCCAATACGATGACAGTTTATTATGTTCATATCATGCTGGGGCAAAAGGTCACTCTATTGACCAGTACCCTTATTTCTCCCAGAAGGTACAAGAATTATTAAATTCTCATTTCCTAACAGTCTCACAGAAGGTAGTTGGAGAATCAGAGCGTGAGCATGAAATAGATGCTGCAGAAGAGTTCTCAACAGGTGAATGCTCGAAGGTTCCATCTCCATTTGAGTATAAAAGTTCAAAAGCAGTACCATGGAATTATGAATATAAGGTACTTGTTGAGTCTGTACCAGTTCCTATAGACAATATTAATGAAATTGGAGGTACAACACGAAGTGGGAAGTGTTATACGCCAGAAGCTTTATTGAAGTACAATAGCAAGGAAAAAGGGAAAGCCAAGCTTAGTGATGTCATTGATTGTAGGATAGAAGACCCACTGTTGACTTATGTTCCATCAGACAATTTAGACAACATCGTTGGCAGTATAATAGCATCAAGTTCCATAACTTTCACTGATGATGAGATACCGCCGGAAGGCATGGGTCACACAAAGGCCTTGCATATTACAGTTAAGTGCAAGAAATTTGCTGTGGCCAAAGTTTTAGTTGACAATGGCTCTTCTTTGAACATACTGCCTATGTCTACATTGGAAAAATTGTCTGTTGATATGTCATACCTCCAATCAAGCACTATGATAGTGAGGGCCTTTGATGGATCGCGCAGTGAAGTGGTTGGGGACATAGAAATCCCTATTCAAATAGGCCCTTGCATCTTTGACATAACTTTCCAAGTCATGAACATCAACTTAGCCTATAGTTTCCTGTTAGGTCGTCCATGGATTCATTCAGCAGGAGTAGTCCCTTCGTCATTGCACCAATGCCTTAAATTTGTAGTCAATCGCAAGCTAGTGATCGTATCAGGACAAGAGGATATTTTTGTGTCAAGCCATCCTCAATGCCATATATAG

Protein sequence

MISRVNHGNTSQLPHPNSGIPHYPHNYYAPPHPYYQPYVNHAAVQYYPPPPQNQRSYVNQGYQPQDVAEVGLRDVVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQYDDSLLCSYHAGAKGHSIDQYPYFSQKVQELLNSHFLTVSQKVVGESEREHEIDAAEEFSTGECSKVPSPFEYKSSKAVPWNYEYKVLVESVPVPIDNINEIGGTTRSGKCYTPEALLKYNSKEKGKAKLSDVIDCRIEDPLLTYVPSDNLDNIVGSIIASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMSTLEKLSVDMSYLQSSTMIVRAFDGSRSEVVGDIEIPIQIGPCIFDITFQVMNINLAYSFLLGRPWIHSAGVVPSSLHQCLKFVVNRKLVIVSGQEDIFVSSHPQCHI

Homology

[Close]

BLAST of Lsi01G018210 vs. ExPASy TrEMBL
Match: A0A6J1CNY7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 4.1e-91
Identity = 215/469 (45.84%), Postives = 262/469 (55.86%), Query Frame = 0


Query: 75  VVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQYDDSLLCSYHAGAKGHSIDQYPYFSQ 134
           V  + T  EELF IL  +  +    L  ++    YD+SL C +HAGAKGH+++Q   F  
Sbjct: 392 VADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHALEQCNSFRM 451

Query: 135 KVQELLNSHFLTVSQKVVGESEREHEIDAAEEFSTGE----------------------- 194
            VQELL+S  LTV+      S ++  I+  E+ S  E                       
Sbjct: 452 IVQELLDSKILTVA-----NSHQKKGINVVEDVSVAEGSIAEGSSDALKPKRLTIFYSEK 511

Query: 195 -----CSK------VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRS 254
                CS+      VP+PFEYKSSKAVPW YE KV     V S P+P+DNI  +GG T +
Sbjct: 512 PDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPLPVDNITGVGGLTXT 571

Query: 255 GKCYTPEALLKYNSK---------------------EKGKAKL-SDVIDCRIE------- 314
           G+CYTP++LLK  S+                     +KGKAKL  DV D  +E       
Sbjct: 572 GRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDELVEAIVVKDV 631

Query: 315 -------------------------------------------------DPLLTY----- 374
                                                            + LL       
Sbjct: 632 SPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSEAHRNTLLEXLKQAF 691

Query: 375 ----VPSDNLDNIVGSIIASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNG 419
               +  DNL N+VG+I ASSSITFTD+EIPPEG GHTKALHI+VKCK F +AKVLVDNG
Sbjct: 692 VSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNG 751

[Close]

BLAST of Lsi01G018210 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 214/459 (46.62%), Postives = 255/459 (55.56%), Query Frame = 0


Query: 75  VVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQYDDSLLCSYHAGAKGHSIDQYPYFSQ 134
           V  + T   ELF IL  +  I    L        YD+SL C +H GAKGHS++Q   F  
Sbjct: 497 VADITTPMXELFEILLGSGYISVEYLCPK--YKGYDESLTCXFHXGAKGHSLEQCNXFRM 556

Query: 135 KVQELLNSHFLT------------VSQKVVGESEREH------EIDAAEEFSTGECSK-- 194
           KVQELL+S  LT            V   +V E   +        I   E+     CS+  
Sbjct: 557 KVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLTIFYREKPDAPSCSRKP 616

Query: 195 ----VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRSGKCYTPEALL 254
               VP PFEYKSSKAVPW YE KV     V S  +P+DNI  +GG TR+G+CYTP++LL
Sbjct: 617 XXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 676

Query: 255 ---------------------KYNSKEKGKAKLSD-----------VIDCRIEDPL---- 314
                                K    +KGKAKL +           V D   + P+    
Sbjct: 677 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEAIVVKDVSPKQPMSEEE 736

Query: 315 ------------------LTYVPS---------------------------------DNL 374
                             L   P+                                 DNL
Sbjct: 737 TQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLEALKQAFVSQDITVDNL 796

Query: 375 DNIVGSIIASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMST 419
            N+VG+I ASSSI+FTD+EIPPEG GHTKALHI+VKCK F +AKVLVDNGSSLNI+P ST
Sbjct: 797 SNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNGSSLNIMPRST 856

[Close]

BLAST of Lsi01G018210 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 1.2e-85
Identity = 203/473 (42.92%), Postives = 258/473 (54.55%), Query Frame = 0


Query: 49  PPPQNQRSYVNQGYQPQDVAEVGLRDVVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQ 108
           P P +Q   +N   + Q++       V  + T   ELF IL  +  +    L  ++    
Sbjct: 434 PLPNHQNVQIN-AIECQEIESKS--KVADIRTPMVELFEILLGSGYVSVEYLCPNLKYKG 493

Query: 109 YDDSLLCSYHAGAKGHSIDQYPYFSQKVQELLNSHFLTVSQK--------VVGESEREHE 168
           YD+SL C +HAGAKGHS++Q   F  KVQELL+S  LTV+          V   S  E  
Sbjct: 494 YDESLTCPFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINIVEDVSVAEGS 553

Query: 169 IDA----------AEEFSTGECSK------VPSPFEYKSSKAVPWNYEYKVL----VESV 228
            DA          +E+ +   CS+      VP+PFEYKSSKAVPW Y+ KV     V S 
Sbjct: 554 SDALKPKCLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSP 613

Query: 229 PVPIDNINEIGGTTRSGKCYTPEALL---------------------KYNSKEKGKAKLS 288
           P+PIDNI  +GG TR+G+CYTP++LL                     K    +KGKAKL 
Sbjct: 614 PLPIDNITGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVEEDKKGKAKLH 673

Query: 289 DVIDCRIEDPLLTY---------------------------------------------- 348
           + +   + + ++                                                
Sbjct: 674 EDVHDELVEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPAKISILSLLLS 733

Query: 349 --------------------VPSDNLDNIVGSIIASSSITFTDDEIPPEGMGHTKALHIT 407
                               +  DNL N+VG+I  +SSITFTD+EIPPEG GHTKALHI+
Sbjct: 734 SEAHRNALLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEGTGHTKALHIS 793

[Close]

BLAST of Lsi01G018210 vs. ExPASy TrEMBL
Match: A0A6J1D7C7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 2.5e-80
Identity = 187/384 (48.70%), Postives = 225/384 (58.59%), Query Frame = 0


Query: 135 KVQELLNSHFLTVSQK------------VVGESEREH------EIDAAEEFSTGECSK-- 194
           KVQELL+S  LTV+              +V E   +        I   E+     CS+  
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKP 61

Query: 195 ----VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRSGKCYTPEALL 254
               VP+PFEYKSSKAVPW YE KV     V S  +P+DNI  +GG TR+G+CYTP++LL
Sbjct: 62  ITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 121

Query: 255 ---------------------KYNSKEKGKAKL-SDVIDCRIEDPLLTY----------- 314
                                K    +KGKAKL  DV D  +E+    +           
Sbjct: 122 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDELVEEETQEFLKLVKQNEYKV 181

Query: 315 ---------------------------------------VPSDNLDNIVGSIIASSSITF 374
                                                  +  DNL N+VG+I+ASS ITF
Sbjct: 182 IEQLGRTPAKISILSLLLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMASSCITF 241

Query: 375 TDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMSTLEKLSVDMSYLQSST 419
           TD+EIPPEG GHTKALHI+VKCK F +AKVLV NGSSLNI+P STLEKL VDMS+++ ST
Sbjct: 242 TDEEIPPEGTGHTKALHISVKCKNFLIAKVLVGNGSSLNIMPRSTLEKLPVDMSHMRPST 301

[Close]

BLAST of Lsi01G018210 vs. ExPASy TrEMBL
Match: A0A5A7T0H8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00180 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 4.9e-76
Identity = 180/452 (39.82%), Postives = 246/452 (54.42%), Query Frame = 0


Query: 46  YYPPPPQNQRSYVNQGYQPQDVAEVGLRDVVRMITSKEELFRILFNNRLIEQGNLQDDVL 105
           Y  P P ++   VN       + E    +V  ++   E LF  LF    +    L  ++ 
Sbjct: 496 YENPLPDHENPKVN---VVDSLVEKCENEVHEIVMPMEALFEGLFEAGYVSHEYLDPNIR 555

Query: 106 VSQYDDSLLCSYHAGAKGHSIDQYPYFSQKVQELLNSHFLTVSQKVVGESEREHE----- 165
              YD+S  C +H G  GH + Q   F  KVQ+L++S  LTV  +  G+ E ++      
Sbjct: 556 YEGYDESRHCIFHRGVAGHVVQQCQKFRSKVQQLMDSKILTV-YRGQGKDEMKYSKICVL 615

Query: 166 IDAAEE-------------------FSTGECS------KVPSPFEYKSSKAVPWNYEYKV 225
           +D   E                    ST  C+      +VPSPF++K  KAVPW Y+ +V
Sbjct: 616 MDEVSEKKDSFLPRPLTVFYQESRNESTSFCNPKKLTIQVPSPFKFKDLKAVPWRYDCQV 675

Query: 226 LVESVPVPIDNINEIGGTTRSGKCYTPEALL---------------KYNSKE-------- 285
           +   +   +DNI EI G TRSG+CY P+ L                K N+KE        
Sbjct: 676 ITGPL---VDNITEISGITRSGRCYKPDNLTVPSNGLILEQGRKNEKRNAKEHCKDQDVE 735

Query: 286 --------------------------KGKAKLSDVIDCRIEDPLLTYVPSDNLDNIVGSI 345
                                     K K+    ++D   +  +   +  +    I+G+I
Sbjct: 736 MPIVAKDIEYKKLVTDEEANEFLKIVKQKSHRKVLLDILNKAHVGHDISVEKFSGIIGNI 795

Query: 346 IASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMSTLEKLSVD 405
            +S+SI FTDDEIPPEG+GHTKALHI +KCK + +A+VLVDNGS+LNI+P STL  L VD
Sbjct: 796 TSSNSIVFTDDEIPPEGLGHTKALHIQLKCKDYVIARVLVDNGSALNIMPKSTLLMLPVD 855

Query: 406 MSYLQSSTMIVRAFDGSRSEVVGDIEIPIQIGPCIFDITFQVMNINLAYSFLLGRPWIHS 419
           MS+++SSTM+V+AFDGSR EV+GDIE+P++IGPCIF+I FQVM I   YSFLLGRPWIHS
Sbjct: 856 MSHIKSSTMVVKAFDGSRREVMGDIELPVKIGPCIFNIVFQVMEITPTYSFLLGRPWIHS 915

[Close]

BLAST of Lsi01G018210 vs. NCBI nr
Match: XP_022143495.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia])

HSP 1 Score: 345.1 bits (884), Expect = 8.5e-91
Identity = 215/469 (45.84%), Postives = 262/469 (55.86%), Query Frame = 0


Query: 75  VVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQYDDSLLCSYHAGAKGHSIDQYPYFSQ 134
           V  + T  EELF IL  +  +    L  ++    YD+SL C +HAGAKGH+++Q   F  
Sbjct: 392 VADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHALEQCNSFRM 451

Query: 135 KVQELLNSHFLTVSQKVVGESEREHEIDAAEEFSTGE----------------------- 194
            VQELL+S  LTV+      S ++  I+  E+ S  E                       
Sbjct: 452 IVQELLDSKILTVA-----NSHQKKGINVVEDVSVAEGSIAEGSSDALKPKRLTIFYSEK 511

Query: 195 -----CSK------VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRS 254
                CS+      VP+PFEYKSSKAVPW YE KV     V S P+P+DNI  +GG T +
Sbjct: 512 PDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPLPVDNITGVGGLTXT 571

Query: 255 GKCYTPEALLKYNSK---------------------EKGKAKL-SDVIDCRIE------- 314
           G+CYTP++LLK  S+                     +KGKAKL  DV D  +E       
Sbjct: 572 GRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDELVEAIVVKDV 631

Query: 315 -------------------------------------------------DPLLTY----- 374
                                                            + LL       
Sbjct: 632 SPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSEAHRNTLLEXLKQAF 691

Query: 375 ----VPSDNLDNIVGSIIASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNG 419
               +  DNL N+VG+I ASSSITFTD+EIPPEG GHTKALHI+VKCK F +AKVLVDNG
Sbjct: 692 VSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNG 751

[Close]

BLAST of Lsi01G018210 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 340.5 bits (872), Expect = 2.1e-89
Identity = 214/459 (46.62%), Postives = 255/459 (55.56%), Query Frame = 0


Query: 75  VVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQYDDSLLCSYHAGAKGHSIDQYPYFSQ 134
           V  + T   ELF IL  +  I    L        YD+SL C +H GAKGHS++Q   F  
Sbjct: 497 VABITTPMXELFEILLGSGYISVEYLCPK--YKGYDESLTCXFHXGAKGHSLEQCNXFRM 556

Query: 135 KVQELLNSHFLT------------VSQKVVGESEREH------EIDAAEEFSTGECSK-- 194
           KVQELL+S  LT            V   +V E   +        I   E+     CS+  
Sbjct: 557 KVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLTIFYREKPDAPSCSRKP 616

Query: 195 ----VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRSGKCYTPEALL 254
               VP PFEYKSSKAVPW YE KV     V S  +P+DNI  +GG TR+G+CYTP++LL
Sbjct: 617 XXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 676

Query: 255 ---------------------KYNSKEKGKAKLSD-----------VIDCRIEDPL---- 314
                                K    +KGKAKL +           V D   + P+    
Sbjct: 677 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEAIVVKDVSPKQPMSEEE 736

Query: 315 ------------------LTYVPS---------------------------------DNL 374
                             L   P+                                 DNL
Sbjct: 737 TQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLEALKQAFVSQDITVDNL 796

Query: 375 DNIVGSIIASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMST 419
            N+VG+I ASSSI+FTD+EIPPEG GHTKALHI+VKCK F +AKVLVDNGSSLNI+P ST
Sbjct: 797 SNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNGSSLNIMPRST 856

[Close]

BLAST of Lsi01G018210 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 327.0 bits (837), Expect = 2.4e-85
Identity = 203/473 (42.92%), Postives = 258/473 (54.55%), Query Frame = 0


Query: 49  PPPQNQRSYVNQGYQPQDVAEVGLRDVVRMITSKEELFRILFNNRLIEQGNLQDDVLVSQ 108
           P P +Q   +N   + Q++       V  + T   ELF IL  +  +    L  ++    
Sbjct: 434 PLPNHQNVQIN-AIECQEIESKS--KVADIRTPMVELFEILLGSGYVSVEYLCPNLKYKG 493

Query: 109 YDDSLLCSYHAGAKGHSIDQYPYFSQKVQELLNSHFLTVSQK--------VVGESEREHE 168
           YD+SL C +HAGAKGHS++Q   F  KVQELL+S  LTV+          V   S  E  
Sbjct: 494 YDESLTCPFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINIVEDVSVAEGS 553

Query: 169 IDA----------AEEFSTGECSK------VPSPFEYKSSKAVPWNYEYKVL----VESV 228
            DA          +E+ +   CS+      VP+PFEYKSSKAVPW Y+ KV     V S 
Sbjct: 554 SDALKPKCLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSP 613

Query: 229 PVPIDNINEIGGTTRSGKCYTPEALL---------------------KYNSKEKGKAKLS 288
           P+PIDNI  +GG TR+G+CYTP++LL                     K    +KGKAKL 
Sbjct: 614 PLPIDNITGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVEEDKKGKAKLH 673

Query: 289 DVIDCRIEDPLLTY---------------------------------------------- 348
           + +   + + ++                                                
Sbjct: 674 EDVHDELVEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPAKISILSLLLS 733

Query: 349 --------------------VPSDNLDNIVGSIIASSSITFTDDEIPPEGMGHTKALHIT 407
                               +  DNL N+VG+I  +SSITFTD+EIPPEG GHTKALHI+
Sbjct: 734 SEAHRNALLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEGTGHTKALHIS 793

[Close]

BLAST of Lsi01G018210 vs. NCBI nr
Match: XP_022150030.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia])

HSP 1 Score: 309.3 bits (791), Expect = 5.1e-80
Identity = 187/384 (48.70%), Postives = 225/384 (58.59%), Query Frame = 0


Query: 135 KVQELLNSHFLTVSQK------------VVGESEREH------EIDAAEEFSTGECSK-- 194
           KVQELL+S  LTV+              +V E   +        I   E+     CS+  
Sbjct: 2   KVQELLDSKILTVANSHQKKRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKP 61

Query: 195 ----VPSPFEYKSSKAVPWNYEYKVL----VESVPVPIDNINEIGGTTRSGKCYTPEALL 254
               VP+PFEYKSSKAVPW YE KV     V S  +P+DNI  +GG TR+G+CYTP++LL
Sbjct: 62  ITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLL 121

Query: 255 ---------------------KYNSKEKGKAKL-SDVIDCRIEDPLLTY----------- 314
                                K    +KGKAKL  DV D  +E+    +           
Sbjct: 122 KRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVHDELVEEETQEFLKLVKQNEYKV 181

Query: 315 ---------------------------------------VPSDNLDNIVGSIIASSSITF 374
                                                  +  DNL N+VG+I+ASS ITF
Sbjct: 182 IEQLGRTPAKISILSLLLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMASSCITF 241

Query: 375 TDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMSTLEKLSVDMSYLQSST 419
           TD+EIPPEG GHTKALHI+VKCK F +AKVLV NGSSLNI+P STLEKL VDMS+++ ST
Sbjct: 242 TDEEIPPEGTGHTKALHISVKCKNFLIAKVLVGNGSSLNIMPRSTLEKLPVDMSHMRPST 301

[Close]

BLAST of Lsi01G018210 vs. NCBI nr
Match: KAA0036942.1 (uncharacterized protein E6C27_scaffold86G00180 [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-75
Identity = 180/452 (39.82%), Postives = 246/452 (54.42%), Query Frame = 0


Query: 46  YYPPPPQNQRSYVNQGYQPQDVAEVGLRDVVRMITSKEELFRILFNNRLIEQGNLQDDVL 105
           Y  P P ++   VN       + E    +V  ++   E LF  LF    +    L  ++ 
Sbjct: 496 YENPLPDHENPKVN---VVDSLVEKCENEVHEIVMPMEALFEGLFEAGYVSHEYLDPNIR 555

Query: 106 VSQYDDSLLCSYHAGAKGHSIDQYPYFSQKVQELLNSHFLTVSQKVVGESEREHE----- 165
              YD+S  C +H G  GH + Q   F  KVQ+L++S  LTV  +  G+ E ++      
Sbjct: 556 YEGYDESRHCIFHRGVAGHVVQQCQKFRSKVQQLMDSKILTV-YRGQGKDEMKYSKICVL 615

Query: 166 IDAAEE-------------------FSTGECS------KVPSPFEYKSSKAVPWNYEYKV 225
           +D   E                    ST  C+      +VPSPF++K  KAVPW Y+ +V
Sbjct: 616 MDEVSEKKDSFLPRPLTVFYQESRNESTSFCNPKKLTIQVPSPFKFKDLKAVPWRYDCQV 675

Query: 226 LVESVPVPIDNINEIGGTTRSGKCYTPEALL---------------KYNSKE-------- 285
           +   +   +DNI EI G TRSG+CY P+ L                K N+KE        
Sbjct: 676 ITGPL---VDNITEISGITRSGRCYKPDNLTVPSNGLILEQGRKNEKRNAKEHCKDQDVE 735

Query: 286 --------------------------KGKAKLSDVIDCRIEDPLLTYVPSDNLDNIVGSI 345
                                     K K+    ++D   +  +   +  +    I+G+I
Sbjct: 736 MPIVAKDIEYKKLVTDEEANEFLKIVKQKSHRKVLLDILNKAHVGHDISVEKFSGIIGNI 795

Query: 346 IASSSITFTDDEIPPEGMGHTKALHITVKCKKFAVAKVLVDNGSSLNILPMSTLEKLSVD 405
            +S+SI FTDDEIPPEG+GHTKALHI +KCK + +A+VLVDNGS+LNI+P STL  L VD
Sbjct: 796 TSSNSIVFTDDEIPPEGLGHTKALHIQLKCKDYVIARVLVDNGSALNIMPKSTLLMLPVD 855

Query: 406 MSYLQSSTMIVRAFDGSRSEVVGDIEIPIQIGPCIFDITFQVMNINLAYSFLLGRPWIHS 419
           MS+++SSTM+V+AFDGSR EV+GDIE+P++IGPCIF+I FQVM I   YSFLLGRPWIHS
Sbjct: 856 MSHIKSSTMVVKAFDGSRREVMGDIELPVKIGPCIFNIVFQVMEITPTYSFLLGRPWIHS 915

The following BLAST results are available for this feature:

BLAST of Lsi01G018210 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi01G018210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNY7	4.1e-91	45.84	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1	[more]
A0A6J1D099	1.3e-89	46.62	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1	[more]
A0A6J1E2J7	1.2e-85	42.92	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]
A0A6J1D7C7	2.5e-80	48.70	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1	[more]
A0A5A7T0H8	4.9e-76	39.82	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of Lsi01G018210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143495.1	8.5e-91	45.84	LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia]	[more]
XP_022147189.1	2.1e-89	46.62	LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia]	[more]
XP_022158986.1	2.4e-85	42.92	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]
XP_022150030.1	5.1e-80	48.70	LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia]	[more]
KAA0036942.1	1.0e-75	39.82	uncharacterized protein E6C27_scaffold86G00180 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi01G018210 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 275..408 e-value: 1.5E-13 score: 52.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 301..389
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	PANTHER	PTHR33240:SF7	GAG-PRO-LIKE PROTEIN	coord: 92..418
None	No IPR available	PANTHER	PTHR33240	OS08G0508500 PROTEIN	coord: 92..418
None	No IPR available	CDD	cd00303	retropepsin_like	coord: 292..385 e-value: 8.27908E-16 score: 70.4432

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G018210.1	Lsi01G018210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process