Lsi01G017970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G017970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF-hand family protein
Location	chr01: 17561186 .. 17561779 (+)
RNA-Seq Expression	Lsi01G017970
Synteny	Lsi01G017970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATCCCCAAAAGATTTAGCCCAAAACGCCTCTTCCGATCCAAGAAGAATCACTCCGCCGACGTCTCGCGCTCCGATCCGACTTCTTTCGGCTCCGGTACGTCTTCTTCCTCTTCTTCTTCCGAGAATTTCTTCAAGCCTAACTCCGCCGCTACCGGTTACGGAACTCCGACAAGTGTCCTCCCCCCGGACTGGTCCGACTCAGATCAGAGAGAAAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTTAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCAGCGATGGATTTATTCGGCTGGAGGAATTGATGGCTCGAATTGGGACGGAGTTCGAAGCGGCGGGAGAGTCGGAGCTGCAGGAGACGTTTGAGTTTTTCGACGCCGATCAGGACGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTCAGATCAATAGGGGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGGTTCGTGTGTTTCGCGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

mRNA sequence

ATGAAAATCCCCAAAAGATTTAGCCCAAAACGCCTCTTCCGATCCAAGAAGAATCACTCCGCCGACGTCTCGCGCTCCGATCCGACTTCTTTCGGCTCCGGTACGTCTTCTTCCTCTTCTTCTTCCGAGAATTTCTTCAAGCCTAACTCCGCCGCTACCGGTTACGGAACTCCGACAAGTGTCCTCCCCCCGGACTGGTCCGACTCAGATCAGAGAGAAAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTTAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCAGCGATGGATTTATTCGGCTGGAGGAATTGATGGCTCGAATTGGGACGGAGTTCGAAGCGGCGGGAGAGTCGGAGCTGCAGGAGACGTTTGAGTTTTTCGACGCCGATCAGGACGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTCAGATCAATAGGGGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGGTTCGTGTGTTTCGCGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

Coding sequence (CDS)

ATGAAAATCCCCAAAAGATTTAGCCCAAAACGCCTCTTCCGATCCAAGAAGAATCACTCCGCCGACGTCTCGCGCTCCGATCCGACTTCTTTCGGCTCCGGTACGTCTTCTTCCTCTTCTTCTTCCGAGAATTTCTTCAAGCCTAACTCCGCCGCTACCGGTTACGGAACTCCGACAAGTGTCCTCCCCCCGGACTGGTCCGACTCAGATCAGAGAGAAAACGACGGCGTTGTGTCGAGGAAGGAGTTGGAGGCGATTCTTAGCCGGATCGCGTCGGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGACGGCGGCAGCGATGGATTTATTCGGCTGGAGGAATTGATGGCTCGAATTGGGACGGAGTTCGAAGCGGCGGGAGAGTCGGAGCTGCAGGAGACGTTTGAGTTTTTCGACGCCGATCAGGACGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTCAGATCAATAGGGGACGAGCGGTGCACGTTAGAGGACTGCCGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGGTTCGTGTGTTTCGCGGAGTTCGTGCGTATGATGGAGCTGCAGAGATGA

Protein sequence

MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTSVLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGFVCFAEFVRMMELQR

Homology

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BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 109/212 (51.42%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MK+ K   PKR F   K+ S  VS+S PT+F  G++SSSS  +          G  TPTS
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTS 60

Query: 61  VLPPDWSDSD-----------QRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDG 120
           +LP   S               R+NDG VSR +LE++LSR+     +EEE+ +ML EVD 
Sbjct: 61  ILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDC 120

Query: 121 GSDGFIRLEELMARIGTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCT 180
             DG IRLEEL +R+ +   A   +EL+ETFEFFDAD+DG I+A+EL  VF +IGDERCT
Sbjct: 121 DGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 180

Query: 181 LEDCRRMIRDVDENGDGFVCFAEFVRMMELQR 198
           L+DC+RMI DVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 9.5e-40
Identity = 109/205 (53.17%), Postives = 135/205 (65.85%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSS-SSENFFKPNSAATGYGTPTSVLPP 65
           R SPKRLFR+K    A VSRS+P+SF S  SSSSS  S    K    A    TP SVLP 
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA----TPISVLPQ 68

Query: 66  DWSD----------SDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDGGSDGF 125
           +  D             R++DGVVSR +L A++SR++    S+EEV++ML EVDGG  G 
Sbjct: 69  NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGC 128

Query: 126 IRLEELMARI-GTEFEAAGES-ELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLED 185
           I LE+L +R+ GT  E + E+ EL+E FE FD D++G+I+AEELH VF  IGDERCTLE+
Sbjct: 129 ISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEE 188

Query: 186 CRRMIRDVDENGDGFVCFAEFVRMM 194
           C RMI  VD NGDGFVCF +F RMM
Sbjct: 189 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 54/128 (42.19%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 72  RENDGVVSRKELEAI---LSRIASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEFEAAG 131
           + NDG +S  EL+ +   LS  AS+EE   M+ E D   +GFI L+E +A      +++ 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 132 ES---ELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGFVC 191
            S   +L+E F+ +D D++GRI+A ELH V +++G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 192 FAEFVRMM 194
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.1e-19
Identity = 56/135 (41.48%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 75  DGVVSRKELEAILSRIAS---EEEVAMMLSEVDGGSDGFIRLEELMA------------- 134
           DG +S +EL ++L  + +     EV  M+ E+D   DGF+ L E  A             
Sbjct: 18  DGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAAFHCGPTPAHGGKG 77

Query: 135 RIGTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDE 194
               + EAA E+EL+E F  +DAD +G+I+A ELH V R +GD +C++ DC RMIR VD 
Sbjct: 78  GDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGD-KCSVADCSRMIRSVDA 137

[Close]

BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 9.3e-19
Identity = 56/124 (45.16%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 75  DGVVSRKELEAI---LSRIASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEF--EAAGE 134
           DG +SR EL A+   L   A+++E+A M++E D   DGFI L+E  A   T     AA E
Sbjct: 53  DGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVE 112

Query: 135 SELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGFVCFAEF 194
            +L+  F  FDAD +G I+A EL  V   +G E+ T++ CRRMI  VD+NGDG + F EF
Sbjct: 113 EDLRHAFRVFDADGNGTISAAELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEF 172

[Close]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.1e-86
Identity = 176/197 (89.34%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDP SFGSGTSSSSSSSENFFKPNS  TGYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G DGFIRLEELMAR+
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+  EAAGESEL+ETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDCRRMIRDVDENG
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENG 181

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCFAEFVRMMELQR
Sbjct: 182 DGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.1e-86
Identity = 176/197 (89.34%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDP SFGSGTSSSSSSSENFFKPNS  TGYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G DGFIRLEELMAR+
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+  EAAGESEL+ETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDCRRMIRDVDENG
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENG 181

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCFAEFVRMMELQR
Sbjct: 182 DGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.5e-84
Identity = 173/197 (87.82%), Postives = 180/197 (91.37%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK+HSADVSRSDP SFGSGTSSSSSSSENFFK N+  TGYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL     DSD R N+GVVSRKELEAILSRIASEEEVAMML EVD G DGFIRLEELMAR+
Sbjct: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARV 121

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+  EAAGESE++ETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDC RMIRDVDENG
Sbjct: 122 GSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENG 181

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCFAEFVRMMELQR
Sbjct: 182 DGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.5e-84
Identity = 172/197 (87.31%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKKN SA+VSRSDP SFGSGTSSSSSSSENFFKP++A  GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL    SDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG  GFIRL+ELMAR+
Sbjct: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+ F+A GESEL+ETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDCRRMIRDVDE+G
Sbjct: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.8e-81
Identity = 171/197 (86.80%), Postives = 181/197 (91.88%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKKN SADVSRSDP SFGSGTSSSSSSSENFFKP++A  GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL    SDSDQREND VVSRKELEAILSRIASEEEVAMMLSEVD G  GFIRL+ELMAR+
Sbjct: 61  VL-AGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG--GFIRLDELMARV 120

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+ F+A GESEL+ETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDCRRMIRDVDE+G
Sbjct: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. NCBI nr
Match: XP_038883626.1 (probable calcium-binding protein CML35 [Benincasa hispida])

HSP 1 Score: 334.7 bits (857), Expect = 5.3e-88
Identity = 178/199 (89.45%), Postives = 183/199 (91.96%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK HSADVSRSDP SFGSGTSSSSSSSE FFKPN+ ATGYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKYHSADVSRSDPPSFGSGTSSSSSSSETFFKPNATATGYGTPTS 60

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVD---GGSDGFIRLEELM 120
           VLPPDW DS QRENDGVVSRKELEAILSRIASEEEVAMMLSEV    GG DGFI LEELM
Sbjct: 61  VLPPDWPDSGQRENDGVVSRKELEAILSRIASEEEVAMMLSEVSSGGGGGDGFIWLEELM 120

Query: 121 ARIGTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVD 180
           A++GT  EAAGESEL+ETFEFFDADQDGRITAEELH VFRSIGDE+CTLEDCRRMIRDVD
Sbjct: 121 AQVGTGVEAAGESELRETFEFFDADQDGRITAEELHEVFRSIGDEQCTLEDCRRMIRDVD 180

Query: 181 ENGDGFVCFAEFVRMMELQ 197
           ENGDG VCFAEFVRMMELQ
Sbjct: 181 ENGDGVVCFAEFVRMMELQ 199

[Close]

BLAST of Lsi01G017970 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 326.2 bits (835), Expect = 1.9e-85
Identity = 176/197 (89.34%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK+ SADVSRSDP SFGSGTSSSSSSSENFFKPNS  TGYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPTS 61

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL     DSD REN+GVV+RKELEAILSRIASEEEVAMMLSEVD G DGFIRLEELMAR+
Sbjct: 62  VL-----DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARV 121

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+  EAAGESEL+ETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDCRRMIRDVDENG
Sbjct: 122 GSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENG 181

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCFAEFVRMMELQR
Sbjct: 182 DGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 319.7 bits (818), Expect = 1.8e-83
Identity = 172/197 (87.31%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKKN SA+VSRSDP SFGSGTSSSSSSSENFFKP++A  GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL    SDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG  GFIRL+ELMAR+
Sbjct: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+ F+A GESEL+ETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDCRRMIRDVDE+G
Sbjct: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lsi01G017970 vs. NCBI nr
Match: XP_004151276.1 (probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetical protein Csa_012346 [Cucumis sativus])

HSP 1 Score: 319.7 bits (818), Expect = 1.8e-83
Identity = 173/197 (87.82%), Postives = 180/197 (91.37%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKK+HSADVSRSDP SFGSGTSSSSSSSENFFK N+  TGYGTPTS
Sbjct: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL     DSD R N+GVVSRKELEAILSRIASEEEVAMML EVD G DGFIRLEELMAR+
Sbjct: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARV 121

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+  EAAGESE++ETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDC RMIRDVDENG
Sbjct: 122 GSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENG 181

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCFAEFVRMMELQR
Sbjct: 182 DGFVCFAEFVRMMELQR 192

[Close]

BLAST of Lsi01G017970 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.8 bits (813), Expect = 6.7e-83
Identity = 171/197 (86.80%), Postives = 182/197 (92.39%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MKIPKRFSPKRLFRSKKN SADVSRSDP SFGSGTSSSSSSSENFFKP++A  GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 61  VLPPDWSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGSDGFIRLEELMARI 120
           VL    SDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG  GFIRL+ELMAR+
Sbjct: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGG--GFIRLDELMARV 120

Query: 121 GTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENG 180
           G+ F++  ESEL+ETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDCRRMIRDVDE+G
Sbjct: 121 GSGFDSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 181 DGFVCFAEFVRMMELQR 198
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 193

[Close]

BLAST of Lsi01G017970 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-42
Identity = 109/212 (51.42%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSSSSENFFKPNSAATGYGTPTS 60
           MK+ K   PKR F   K+ S  VS+S PT+F  G++SSSS  +          G  TPTS
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTS 60

Query: 61  VLPPDWSDSD-----------QRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDG 120
           +LP   S               R+NDG VSR +LE++LSR+     +EEE+ +ML EVD 
Sbjct: 61  ILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDC 120

Query: 121 GSDGFIRLEELMARIGTEFEAAGESELQETFEFFDADQDGRITAEELHGVFRSIGDERCT 180
             DG IRLEEL +R+ +   A   +EL+ETFEFFDAD+DG I+A+EL  VF +IGDERCT
Sbjct: 121 DGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 180

Query: 181 LEDCRRMIRDVDENGDGFVCFAEFVRMMELQR 198
           L+DC+RMI DVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Lsi01G017970 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 164.9 bits (416), Expect = 6.8e-41
Identity = 109/205 (53.17%), Postives = 135/205 (65.85%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNHSADVSRSDPTSFGSGTSSSSS-SSENFFKPNSAATGYGTPTSVLPP 65
           R SPKRLFR+K    A VSRS+P+SF S  SSSSS  S    K    A    TP SVLP 
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA----TPISVLPQ 68

Query: 66  DWSD----------SDQRENDGVVSRKELEAILSRIA----SEEEVAMMLSEVDGGSDGF 125
           +  D             R++DGVVSR +L A++SR++    S+EEV++ML EVDGG  G 
Sbjct: 69  NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGC 128

Query: 126 IRLEELMARI-GTEFEAAGES-ELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLED 185
           I LE+L +R+ GT  E + E+ EL+E FE FD D++G+I+AEELH VF  IGDERCTLE+
Sbjct: 129 ISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEE 188

Query: 186 CRRMIRDVDENGDGFVCFAEFVRMM 194
           C RMI  VD NGDGFVCF +F RMM
Sbjct: 189 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Lsi01G017970 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 98.2 bits (243), Expect = 7.8e-21
Identity = 54/128 (42.19%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 72  RENDGVVSRKELEAI---LSRIASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEFEAAG 131
           + NDG +S  EL+ +   LS  AS+EE   M+ E D   +GFI L+E +A      +++ 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 132 ES---ELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGFVC 191
            S   +L+E F+ +D D++GRI+A ELH V +++G E+C+++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 192 FAEFVRMM 194
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of Lsi01G017970 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 92.0 bits (227), Expect = 5.6e-19
Identity = 53/130 (40.77%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 72  RENDGVVSRKELEAI---LSRIASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEFEAAG 131
           +  DG +S  EL+ +   LS  AS EE   M+ + D   +GFI L+E +A         G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 132 E-----SELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGF 191
                 S+L+E FE +D D +GRI+A+ELH V +++G E+C+++DC++MI  VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

Query: 192 VCFAEFVRMM 194
           V F EF +MM
Sbjct: 147 VNFDEFKKMM 155

[Close]

BLAST of Lsi01G017970 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 88.2 bits (217), Expect = 8.0e-18
Identity = 51/126 (40.48%), Postives = 78/126 (61.90%), Query Frame = 0


Query: 75  DGVVSRKELEAILSRI---ASEEEVAMMLSEVDGGSDGFIRLEELMARIGTEFEAAGE-- 134
           DG +S KEL AI++ +     EEE+   ++E+D   DG+I  EE +  + T+     +  
Sbjct: 50  DGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFV-ELNTKGMDQNDVL 109

Query: 135 SELQETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCRRMIRDVDENGDGFVCFAEF 194
             L++ F  +D D +G I+AEELH V RS+GDE C++ +CR+MI  VD++GDG + F EF
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

Query: 195 VRMMEL 196
             MM +
Sbjct: 170 KIMMTM 173

The following BLAST results are available for this feature:

BLAST of Lsi01G017970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	2.3e-41	51.42	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	9.5e-40	53.17	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9C8Y1	1.1e-19	42.19	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q0DJV6	7.1e-19	41.48	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RZB5	9.3e-19	45.16	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lsi01G017970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VA05	9.1e-86	89.34	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	9.1e-86	89.34	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]
A0A0A0L0H1	8.5e-84	87.82	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]
A0A6J1F310	8.5e-84	87.31	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	1.8e-81	86.80	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]

BLAST of Lsi01G017970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883626.1	5.3e-88	89.45	probable calcium-binding protein CML35 [Benincasa hispida]	[more]
XP_008457589.1	1.9e-85	89.34	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]
XP_022932888.1	1.8e-83	87.31	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_004151276.1	1.8e-83	87.82	probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetica...	[more]
XP_023543601.1	6.7e-83	86.80	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi01G017970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	1.6e-42	51.42	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	6.8e-41	53.17	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	7.8e-21	42.19	calmodulin like 23	[more]
AT5G37770.1	5.6e-19	40.77	EF hand calcium-binding protein family	[more]
AT1G24620.1	8.0e-18	40.48	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 131..159 e-value: 3.6E-5 score: 33.2 coord: 168..196 e-value: 1.6E-5 score: 34.3 coord: 95..123 e-value: 6.5 score: 11.2
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 75..118 e-value: 0.009 score: 15.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 130..193 e-value: 3.7E-10 score: 40.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 164..197 score: 13.14653
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 14.597092
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..193 e-value: 1.36294E-17 score: 71.4249
None	No IPR available	GENE3D	1.10.238.10		coord: 122..197 e-value: 4.7E-20 score: 74.0
None	No IPR available	GENE3D	1.10.238.10		coord: 68..118 e-value: 9.4E-7 score: 30.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..67
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..195
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..195
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 177..189
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 73..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G017970.1	Lsi01G017970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding