Lsi01G016540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G016540
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAmino acid transporter, putative
Locationchr01: 15083702 .. 15087039 (-)
RNA-Seq ExpressionLsi01G016540
SyntenyLsi01G016540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCTTCTTCATTCATCTTGAACCATAGCCAACAATCTCCAACAATGGAGCTTCCCACCGATACTGTAGAGATTCCTCTGCTCAATTCTTCTTCTTCTTCTCCACGAACCACTTCCACTCTTCAAGCTCTTGGAAACATCATCGTCTCCGTCGTCGGCACCGGCGTCTTAGGCTTGCCTTTTGCTTTCAGAATCGCCGGTTATGCTGCCGGATCCTTTGGCGTCCTACTCGCCGCCGTCGCCACCTACTATTGTATGCTCCTTCTGGTTCGTTTCGTTTTCTTCTTGTTTCTTTGTTTTATATTTAGATTCATAGAGGCAACTGGATTTTGGTTAATGATCAACCTGTTCGGTTGATGAAATTGGATTCATAACCTTTCGTGATTGCGTTAGGTGTAGTGTTAGCCTATTTGGCGAATTTGATTTTTCTGAATTCGAGAGAATGTTTTCGGTGCTTCTACTTCGCTTTCTTTCTACATTGTTAACTAATGGACAGTTTGAATGTTGTTGTTTGCCTAAATGTTTGAATTATTATTGTGTTTGATTTCCTCGGAATGTTGTATTTTTCTTCTTGTTAACTATTGAAATGTTTTAATACCGTTTTATTTGTAAATGTTTGAATCATTGTTGTTGATTCCTTGGAATGCTATTTTGAAGTTGTATGTGTAAATGTTTGAATGTTGTTGTTTGCCGAAATGTTTGAATTATTATTGTGTTTGATTCCTTGAAATGCTGTATTTTTCTTTTTGTTAACTATTGACATGTTCGAATGCTGTTGTAGGCCTAAATGTTTGAATTACTGTTGTTGATTCCTTGAAATGCTATTTTGAAGTTGTATGTGTAGATGTTTGAATGTTGTTGTATGCTTAATGTTTGAATTATTGTGTTCGATTCCTCGGAAAGCTATTACGGTTAGGTATAGTGTTAATTTGATATATATTTTTCTTTATTTTCTTTGTTCAACCTTGTTAACTATTGAAACGTTTGAGAATGCTGTGGTATGCGTAAACGAATTATTGTTGTTGATTCCTTGGAATGCTATTTTGAAGTTGTATGTGTAAATGTTTGAATTATTGTGTTTGATTCCTTGGAGAGCTATTACGGTTAAGTATAGTGTTAATTTGATATATATTTTTCTTAATTTTCATTTTTCAACCTTGCTAATTATTGAAATGTTTGGATGTTTTTGTTTGTGCAAATTTTCGAATTATTGCTCTTGATTCCTTGGAATGCTATTTTGAAGTTGGAGTTCAATTCAGTTTTATCGTTTATGTAAGGTGATCCCATGTTCGGGTGATGAAATTGATTAACATTCATAAGCTTTCATGGTTAAGTAACGTTAAGGTATACTGTTGACCTGTTTGGCGAATTGATTTTCTGAATTGGAGAGAATCTGTTCGGTGTTTCTACTGCGCTTTTCTTCTACAGTGTTAACTAATGATATGTTTGAATGTTTGAATTATTGTGTTCGATGCCTTTGTAAAGCTTTTCTGAAGCTGTAGGTCTGTTCAATTTTCTTGTCATGTAAGGTGAAGTGTAGAGAGAAATCGACGTTGCAAGGAAGATCCAACGAATCAAGAACATATGGGGACTTGGGATACATATGCATGGGAAACAAAGGTCGATACCTTACGGAGTTTCTCATTTTCTTTGCCCAATGTGGAGGCTCGGTGGCATACCTGGTATTTATTGGACAAAACCTTTCCTCTGTATTTCAAGGTCATGGCATCGCATTATCCTCCTATATATTTTTGATAGCAGCAGTTGAAGTTGTGTTGTCATGGATTGGAAGTCTGGCTGCTTTAGCACCCTTCAGTATCTTTGCTGATATTTGTAATGCAATAGCAATGGGAATAGTGGTGAAGGAAGATATACAAAAAGCAATAGCCGGTGGAATTTCGTTTAAGGAAAGAACAGCAATTACATCAAATTTGCGAGGTTTGCCATTTGCAGGAGGGATGGCAGTGTTCTGTTTTGAAGGATTTGGCATGACATTGGCTTTGGAGTCCTCTATGAAAGACAAGTCTGCATTCCCAAAAGTTCTTGGTCAGGCTCTAGTAGGAATAACCATTGTGTACGTTTTGTTTGGATTCTCTGGATACATGGCTTATGGTGATGATACCAGAGATATTATCACTCTGAATCTTCCCGATACTTGGTCTACAAAAGCAGTTCAGGTAAGCATCGGTAGGGATGCGCTACTAATTTCTCATATTTTATTATTCTTCCATGGTAATGTTTAGATAACTTCTCATTCACTTTCCACCATATCATCCTCTAGATGAGGTTTCAGAATTAGACAAGATCAAGCCCATTTGTGTTCTTGTTCTCATTCACGTCTTTGAGTAACCGTTTAAACTTATGACAAGATTTCCTCTGGGGAATAAATTATAAATCATTAGCTAGCTGTATGCTTTGTCATTATGTCACATCACATGCTACTCGTTGCAAGTGAGCAGAATTTGTCATTTTCATGTAGTTGTCCTTGTCCTCTCTTATGCATGTGCTGCTGATTGAATTTATCTCTTTTATTTTCTTTTTTTCATTGCTTGATTAGTTTCCTTGATTTGAGGTATTGTCAATGATCGATGGGAATATTGCATTTGGGCCGTCCTGCTCAACAGTTGAATCACCTATTGGACTTTAACACTACCATATTTTCACAACAAATTAATGAACATTCAAAATTGTTCATAAGCAATATTGTCAAAAGACTCTTTCTGATGCTATCAAATCTTGAGTTCTTTCTCCCCCTCTACTCTAAAAAAGCATGGAATTTAAAATTTTGATCTGTATTTCTCTGTATTTGCATTGCATCAGTATGACGTTCGAGAAAATAAAGCAAACTTTCTGACATGAATCTTAAACGTGCTTCAGGTCGGATTGTGTGTGGGTCTAGTTTTCACATTCCCTATGATGTTGCATCCAATTAACGAGATTGTGGAGGGTAAGTTGAAACAAAGCAACTGGTATGAGAAGATTGAAGACAATGATAATATATTTTCAGGAAAACGAGTGAAGGTAGCTACGTACATCAGTCGGGCGATCATCGTGCTTGGATTGGCGATCTTGGCTTCATTTGTTCCAGGTTTTGGTGTTTTTGCCTCACTTGTTGGCAGTACTATATGTGCTTTAATCTCATTTGTTTTGCCTGCCATTTTTCACCTAATGTTGATGGGTTCTTCACTAAGCTTGGGGCAAAAAGTTTTGGATTCTTTCATTTTGATATGTGGATTGCTTTTTGCAGCTTATGGTACTTACAATTCTCTCGTTGGTATTTGAGCTGCTGTTTACTTGTAACTTAAAATTACATTTTTATAGTAAAATGGCTGTTTGTAC

mRNA sequence

ATCCTTCTTCATTCATCTTGAACCATAGCCAACAATCTCCAACAATGGAGCTTCCCACCGATACTGTAGAGATTCCTCTGCTCAATTCTTCTTCTTCTTCTCCACGAACCACTTCCACTCTTCAAGCTCTTGGAAACATCATCGTCTCCGTCGTCGGCACCGGCGTCTTAGGCTTGCCTTTTGCTTTCAGAATCGCCGGTTATGCTGCCGGATCCTTTGGCGTCCTACTCGCCGCCGTCGCCACCTACTATTGTATGCTCCTTCTGGTGAAGTGTAGAGAGAAATCGACGTTGCAAGGAAGATCCAACGAATCAAGAACATATGGGGACTTGGGATACATATGCATGGGAAACAAAGGTCGATACCTTACGGAGTTTCTCATTTTCTTTGCCCAATGTGGAGGCTCGGTGGCATACCTGGTATTTATTGGACAAAACCTTTCCTCTGTATTTCAAGGTCATGGCATCGCATTATCCTCCTATATATTTTTGATAGCAGCAGTTGAAGTTGTGTTGTCATGGATTGGAAGTCTGGCTGCTTTAGCACCCTTCAGTATCTTTGCTGATATTTGTAATGCAATAGCAATGGGAATAGTGGTGAAGGAAGATATACAAAAAGCAATAGCCGGTGGAATTTCGTTTAAGGAAAGAACAGCAATTACATCAAATTTGCGAGGTTTGCCATTTGCAGGAGGGATGGCAGTGTTCTGTTTTGAAGGATTTGGCATGACATTGGCTTTGGAGTCCTCTATGAAAGACAAGTCTGCATTCCCAAAAGTTCTTGGTCAGGCTCTAGTAGGAATAACCATTGTGTACGTTTTGTTTGGATTCTCTGGATACATGGCTTATGGTGATGATACCAGAGATATTATCACTCTGAATCTTCCCGATACTTGGTCTACAAAAGCAGTTCAGGTCGGATTGTGTGTGGGTCTAGTTTTCACATTCCCTATGATGTTGCATCCAATTAACGAGATTGTGGAGGGTAAGTTGAAACAAAGCAACTGGTATGAGAAGATTGAAGACAATGATAATATATTTTCAGGAAAACGAGTGAAGGTAGCTACGTACATCAGTCGGGCGATCATCGTGCTTGGATTGGCGATCTTGGCTTCATTTGTTCCAGGTTTTGGTGTTTTTGCCTCACTTGTTGGCAGTACTATATGTGCTTTAATCTCATTTGTTTTGCCTGCCATTTTTCACCTAATGTTGATGGGTTCTTCACTAAGCTTGGGGCAAAAAGTTTTGGATTCTTTCATTTTGATATGTGGATTGCTTTTTGCAGCTTATGGTACTTACAATTCTCTCGTTGGTATTTGAGCTGCTGTTTACTTGTAACTTAAAATTACATTTTTATAGTAAAATGGCTGTTTGTAC

Coding sequence (CDS)

ATGGAGCTTCCCACCGATACTGTAGAGATTCCTCTGCTCAATTCTTCTTCTTCTTCTCCACGAACCACTTCCACTCTTCAAGCTCTTGGAAACATCATCGTCTCCGTCGTCGGCACCGGCGTCTTAGGCTTGCCTTTTGCTTTCAGAATCGCCGGTTATGCTGCCGGATCCTTTGGCGTCCTACTCGCCGCCGTCGCCACCTACTATTGTATGCTCCTTCTGGTGAAGTGTAGAGAGAAATCGACGTTGCAAGGAAGATCCAACGAATCAAGAACATATGGGGACTTGGGATACATATGCATGGGAAACAAAGGTCGATACCTTACGGAGTTTCTCATTTTCTTTGCCCAATGTGGAGGCTCGGTGGCATACCTGGTATTTATTGGACAAAACCTTTCCTCTGTATTTCAAGGTCATGGCATCGCATTATCCTCCTATATATTTTTGATAGCAGCAGTTGAAGTTGTGTTGTCATGGATTGGAAGTCTGGCTGCTTTAGCACCCTTCAGTATCTTTGCTGATATTTGTAATGCAATAGCAATGGGAATAGTGGTGAAGGAAGATATACAAAAAGCAATAGCCGGTGGAATTTCGTTTAAGGAAAGAACAGCAATTACATCAAATTTGCGAGGTTTGCCATTTGCAGGAGGGATGGCAGTGTTCTGTTTTGAAGGATTTGGCATGACATTGGCTTTGGAGTCCTCTATGAAAGACAAGTCTGCATTCCCAAAAGTTCTTGGTCAGGCTCTAGTAGGAATAACCATTGTGTACGTTTTGTTTGGATTCTCTGGATACATGGCTTATGGTGATGATACCAGAGATATTATCACTCTGAATCTTCCCGATACTTGGTCTACAAAAGCAGTTCAGGTCGGATTGTGTGTGGGTCTAGTTTTCACATTCCCTATGATGTTGCATCCAATTAACGAGATTGTGGAGGGTAAGTTGAAACAAAGCAACTGGTATGAGAAGATTGAAGACAATGATAATATATTTTCAGGAAAACGAGTGAAGGTAGCTACGTACATCAGTCGGGCGATCATCGTGCTTGGATTGGCGATCTTGGCTTCATTTGTTCCAGGTTTTGGTGTTTTTGCCTCACTTGTTGGCAGTACTATATGTGCTTTAATCTCATTTGTTTTGCCTGCCATTTTTCACCTAATGTTGATGGGTTCTTCACTAAGCTTGGGGCAAAAAGTTTTGGATTCTTTCATTTTGATATGTGGATTGCTTTTTGCAGCTTATGGTACTTACAATTCTCTCGTTGGTATTTGA

Protein sequence

MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI
Homology
BLAST of Lsi01G016540 vs. ExPASy Swiss-Prot
Match: Q9SF09 (Amino acid transporter ANT1 OS=Arabidopsis thaliana OX=3702 GN=ANT1 PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.9e-149
Identity = 267/423 (63.12%), Postives = 334/423 (78.96%), Query Frame = 0

Query: 5   TDTVEIPLLNSSSSSPR---TTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVL 64
           T    +PL+ S  S       TS LQ LGNIIVS+VGTGVLGLP+AFRIAG+ AGS GV+
Sbjct: 10  TGDSSLPLIKSPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGWLAGSLGVI 69

Query: 65  LAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGS 124
           +   ATYYCMLLL++CR+K   +    ES+TYGDLG+ CMG KGRYLTEFLIF AQCGGS
Sbjct: 70  IVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLIFTAQCGGS 129

Query: 125 VAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAM 184
           VAYLVFIG+NLSS+F  +G+++ S+I ++  +EV LSWI SL+AL+PFSIFADICN IAM
Sbjct: 130 VAYLVFIGRNLSSIFSSYGLSMVSFILILVPIEVGLSWITSLSALSPFSIFADICNIIAM 189

Query: 185 GIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSA 244
             VVKE+++  I G  SF +RTAI+S + GLPFAGG+AVFCFEGF MTLALESSM+++ A
Sbjct: 190 CFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFCFEGFAMTLALESSMREREA 249

Query: 245 FPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTF 304
           FPK+L + L GIT VYVLFGF GYMAYGD T+DIITLNLP+ WS  AVQ+GLCVGL FTF
Sbjct: 250 FPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPNNWSAIAVQIGLCVGLTFTF 309

Query: 305 PMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPG 364
           P+M+HP+NEI+E KLK+ +W +K  +  +  +G   K A + +R ++V+GLA +AS VPG
Sbjct: 310 PIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIFTTRTLLVVGLAAIASLVPG 369

Query: 365 FGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSL 424
           FG FASLVGST+CALISFVLPA +HL L+G SL++  K +D FI+ICGL+FA YGTYN++
Sbjct: 370 FGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSIDVFIVICGLIFAVYGTYNTI 429

BLAST of Lsi01G016540 vs. ExPASy Swiss-Prot
Match: F4ILY9 (Amino acid transporter AVT3B OS=Arabidopsis thaliana OX=3702 GN=AVT3B PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-60
Identity = 153/415 (36.87%), Postives = 230/415 (55.42%), Query Frame = 0

Query: 23  TSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVATYYCMLLLVKCREKST 82
           +S  +   N+ +++VG GVLGLP+AF+  G+  G   +   A    +CM+LLV  R K  
Sbjct: 23  SSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLFSVAALINHCMMLLVHIRRK-- 82

Query: 83  LQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVFIGQNLSSVFQG---- 142
             G SN   ++GDLG+   GN GR++ + LI  +Q G  V YL+FIG  L+++ +     
Sbjct: 83  -LGVSNIG-SFGDLGFAACGNLGRFVVDILIILSQAGFCVGYLIFIGNTLANLSKPTKST 142

Query: 143 --------HGIALSS-YIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKEDI 202
                    G++  S YI+     ++ L+ I +L  LAP SIFAD+ +  AM +V+ EDI
Sbjct: 143 TLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLGAMAVVIVEDI 202

Query: 203 QKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLGQA 262
           +  +      + +      +    +  G+AV+ FEG GM L LES  KDK  F KVL  +
Sbjct: 203 KITVVQ----RPQVVAFGGMSVFFYGMGVAVYAFEGVGMVLPLESETKDKDKFGKVLALS 262

Query: 263 LVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMMLHPIN 322
           ++ I ++Y  FG  GYMA+GDDT DIIT NL     +  VQ+GLC+ L FTFP+M++P+ 
Sbjct: 263 MLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVSSLVQLGLCINLFFTFPLMMNPVF 322

Query: 323 EIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFASLV 382
           EIVE +                +SG    +     R ++VL + ++A  VP F  F SLV
Sbjct: 323 EIVERR---------------FWSG----MYCVWLRWLLVLAVTLVALLVPNFADFLSLV 382

Query: 383 GSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI 425
           GS++C  + FVLP++FHLM+    +   Q+ LD  IL+ G++    GT++SL  I
Sbjct: 383 GSSVCCALGFVLPSLFHLMVFKDEMEWKQRALDVGILLLGVILGVSGTWSSLTEI 410

BLAST of Lsi01G016540 vs. ExPASy Swiss-Prot
Match: Q9SVG0 (Amino acid transporter AVT3C OS=Arabidopsis thaliana OX=3702 GN=AVT3C PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.6e-60
Identity = 154/439 (35.08%), Postives = 238/439 (54.21%), Query Frame = 0

Query: 4   PTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLA 63
           P    + PLL      P  +S  +   N+ ++VVG GVLGLP+AF+  G+  G   ++  
Sbjct: 18  PPAREDSPLL---GKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFKRTGWLMGVLLLVSV 77

Query: 64  AVATYYCMLLLVKCREK-STLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSV 123
           +V T++CM+LLV  R K  +     ++  ++GDLG+   G+ GR + +  I  +Q G  V
Sbjct: 78  SVLTHHCMMLLVYTRRKLDSFNAGISKIGSFGDLGFAVCGSLGRIVVDLFIILSQAGFCV 137

Query: 124 AYLVFIGQNLSSVFQGH-----------------GIALSS-YIFLIAAVEVVLSWIGSLA 183
            YL+FIG  L+++                     G++  S YI+     ++ L+ I +L 
Sbjct: 138 GYLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGCFPFQLGLNSIKTLT 197

Query: 184 ALAPFSIFADICNAIAMGIVVKEDIQKAIAGGISFKERTAITS--NLRGLPFAGGMAVFC 243
            LAP SIFADI +  AM +V+ ED        I  K+R  + +   +    +  G+AV+ 
Sbjct: 198 HLAPLSIFADIVDLGAMAVVIVEDSM------IILKQRPDVVAFGGMSLFLYGMGVAVYS 257

Query: 244 FEGFGMTLALESSMKDKSAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPD 303
           FEG GM L LES MKDK  F KVL   +  I+++Y+ FG  GY+A+G+DT DIIT NL  
Sbjct: 258 FEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMDIITANLGA 317

Query: 304 TWSTKAVQVGLCVGLVFTFPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATY 363
              +  VQ+GLC+ L FTFP+M++P+ EIVE +  +  +   +                 
Sbjct: 318 GLVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWL----------------- 377

Query: 364 ISRAIIVLGLAILASFVPGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLD 422
             R ++VL + ++A FVP F  F SLVGS+ C ++ FVLPA+FHL++    +   Q   D
Sbjct: 378 --RWVLVLAVTLVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSD 428

BLAST of Lsi01G016540 vs. ExPASy Swiss-Prot
Match: Q9FKY3 (Amino acid transporter AVT3A OS=Arabidopsis thaliana OX=3702 GN=AVT3A PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.0e-55
Identity = 148/422 (35.07%), Postives = 227/422 (53.79%), Query Frame = 0

Query: 7   TVEIPLLNSSSSSPRTTSTL-QALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAV 66
           T + PLL      PRT S+  +   N+ +++VG GVLGLP+ F+  G+  G   +L  + 
Sbjct: 26  TEDTPLL-----GPRTLSSQPKTFANVFIAIVGAGVLGLPYTFKKTGWLLGLLTLLFVSS 85

Query: 67  ATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYL 126
            T++CM+LLV  R K       N   ++GDLG    G  GR + + ++  +Q G  V+YL
Sbjct: 86  LTFFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPAGRLVVDVMLVLSQSGFCVSYL 145

Query: 127 VFIG---QNLSSVFQGHGIAL---SSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAI 186
           +F+     NL S    H + L   S Y++     ++ L+ I SL  LAP SIFADI +  
Sbjct: 146 IFVATTMANLLSRGTEHILGLDAASIYLWGCFPFQLGLNSIPSLTHLAPLSIFADIVDVA 205

Query: 187 AMGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDK 246
           A  +V+ +D+   +      +    ++    GL    G+AV+ FEG GM L LE   K K
Sbjct: 206 ATLVVMVQDVFIFLKRRPPLRVFGGVSVFFYGL----GVAVYAFEGIGMVLPLELEAKYK 265

Query: 247 SAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVF 306
             F + LG A+  I+I+Y  FG  GYMAYG++T+DIIT NL     +  VQ+GL + L F
Sbjct: 266 DKFGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIITTNLGTGVVSTLVQLGLAINLFF 325

Query: 307 TFPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFV 366
           TFP+M+ P+ E+VE +L  S +   +                   R   VL + ++A  V
Sbjct: 326 TFPLMMQPVYEVVERRLCSSRYSVWV-------------------RWATVLVVTLVALLV 385

Query: 367 PGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYN 422
           P F  F SLVGS++C ++ FVLP++FHL    + LS+ + V+D  + + G++ A  GT+ 
Sbjct: 386 PNFADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITGTWT 419

BLAST of Lsi01G016540 vs. ExPASy Swiss-Prot
Match: Q495N2 (Proton-coupled amino acid transporter 3 OS=Homo sapiens OX=9606 GN=SLC36A3 PE=2 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 9.2e-32
Identity = 118/427 (27.63%), Postives = 199/427 (46.60%), Query Frame = 0

Query: 24  STLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVATYYCMLLLVKCREKSTL 83
           S +Q L +++   +GTG+LGLP A + AG   G   +L   V T +CM++L+ C +  + 
Sbjct: 43  SMMQTLIHLLKCNIGTGLLGLPLAIKNAGLLVGPVSLLAIGVLTVHCMVILLNCAQHLSQ 102

Query: 84  QGRS-----NESRTYG-----DLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVFIGQNL- 143
           + +       E+  YG     +         GRY   FL+   Q G    Y +F+  NL 
Sbjct: 103 RLQKTFVNYGEATMYGLETCPNTWLRAHAVWGRYTVSFLLVITQLGFCSVYFMFMADNLQ 162

Query: 144 ---------SSVFQGHGIA-------LSSYIFLIAAVEVVLSWIGSLAALAPFSIFADIC 203
                    S++ Q   I        +  Y+ +I    ++L +I +L  L+ FS  A+I 
Sbjct: 163 QMVEKAHVTSNICQPREILTLTPILDIRFYMLIILPFLILLVFIQNLKVLSVFSTLANIT 222

Query: 204 NAIAMGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSM 263
              +M ++      + I  GI +     + +N +      G A+F FEG GM L L++ M
Sbjct: 223 TLGSMALIF-----EYIMEGIPYPSNLPLMANWKTFLLFFGTAIFTFEGVGMVLPLKNQM 282

Query: 264 KDKSAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVG 323
           K    F  VL   +  + I+Y+L G  GYM +G DT+  ITLNLP+ W  ++V++   +G
Sbjct: 283 KHPQQFSFVLYLGMSIVIILYILLGTLGYMKFGSDTQASITLNLPNCWLYQSVKLMYSIG 342

Query: 324 LVFTFPMMLH-PINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAIL 383
           + FT+ +  H P   I+   + Q      + ++  +F    V       R+ +V    + 
Sbjct: 343 IFFTYALQFHVPAEIIIPFAISQ------VSESWALFVDLSV-------RSALVCLTCVS 402

Query: 384 ASFVPGFGVFASLVGSTICALISFVLPAIFHLMLMGS-SLSLGQKVLDSFILICGLLFAA 422
           A  +P   +  SLVGS   + ++ ++PA+  +++  S  +S      D  I I GLL   
Sbjct: 403 AILIPRLDLVISLVGSVSSSALALIIPALLEIVIFYSEDMSCVTIAKDIMISIVGLLGCI 451

BLAST of Lsi01G016540 vs. ExPASy TrEMBL
Match: A0A1S3B5C4 (amino acid transporter ANT1 OS=Cucumis melo OX=3656 GN=LOC103485964 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 6.1e-212
Identity = 390/424 (91.98%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTDT+EIPLLNSSS   RTTST QAL NIIVSVVGTGVLGLPFAFRIAGYAAGSFGV
Sbjct: 1   MELPTDTMEIPLLNSSS---RTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
           LL A+ATYYCMLLLVKCREK TLQGRSNES+TYGDLGYICMGNKGRYLTEFLIFFAQCGG
Sbjct: 61  LLVALATYYCMLLLVKCREKLTLQGRSNESKTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHGI LSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA
Sbjct: 121 SVAYLVFIGQNLSSVFQGHGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDK+
Sbjct: 181 MGIVVKEDIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKA 240

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVLGQALVGITIVY+LFGFSGYMAYGDDTRDIITLNLP+TWSTKAVQVGLCVGL+FT
Sbjct: 241 AFPKVLGQALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLIFT 300

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FP+MLHPINEIVEGKL QSNW+EKIEDND IF GKR + ATY+SRA+IVL LAILASFVP
Sbjct: 301 FPIMLHPINEIVEGKLAQSNWFEKIEDNDVIFLGKRAQAATYMSRAVIVLVLAILASFVP 360

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFGVFASLVGSTICALISFVLPAIFHLMLMGSSL L QKVLDS ILICGL+FA YGTYNS
Sbjct: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAVYGTYNS 420

Query: 421 LVGI 425
           L GI
Sbjct: 421 LFGI 421

BLAST of Lsi01G016540 vs. ExPASy TrEMBL
Match: A0A0A0L026 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G193760 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.8e-209
Identity = 385/417 (92.33%), Postives = 399/417 (95.68%), Query Frame = 0

Query: 8   VEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVAT 67
           ++IPLLNSSSSS  TTST QAL NIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLL A+AT
Sbjct: 1   MDIPLLNSSSSSSPTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLVALAT 60

Query: 68  YYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF 127
           YYCMLLLVKCREK  LQGRS ES+TYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF
Sbjct: 61  YYCMLLLVKCREKLALQGRSKESQTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF 120

Query: 128 IGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE 187
           IGQNLSSVFQ +GI LSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE
Sbjct: 121 IGQNLSSVFQSYGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE 180

Query: 188 DIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLG 247
           DIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDK+AFPKVLG
Sbjct: 181 DIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKAAFPKVLG 240

Query: 248 QALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMMLHP 307
           QALVGITIVY+LFGFSGYMAYGDDTRDIITLNLP+TWSTKAVQVGLCVGLVFTFP+MLHP
Sbjct: 241 QALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLVFTFPIMLHP 300

Query: 308 INEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFAS 367
           INEIVEGKL QSNW+EKIEDND+IFSGKR KVATYISR +IVL LAILASFVPGFGVFAS
Sbjct: 301 INEIVEGKLAQSNWFEKIEDNDDIFSGKRAKVATYISRTLIVLVLAILASFVPGFGVFAS 360

Query: 368 LVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI 425
           LVGSTICALISFVLPAIFHLMLMGSSL L QKVLDS ILICGL+FAAYGTYNSLVGI
Sbjct: 361 LVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAAYGTYNSLVGI 417

BLAST of Lsi01G016540 vs. ExPASy TrEMBL
Match: A0A6J1ISA2 (amino acid transporter ANT1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478243 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.0e-206
Identity = 378/424 (89.15%), Postives = 403/424 (95.05%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTD VEIPLL+  SSSPRTTSTLQALGNIIVSVVGTG+LGLP+AFRIAG+ AGSFGV
Sbjct: 12  MELPTDAVEIPLLH--SSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV 71

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
            LA VATYYCMLLLVKCREK T QGRS ESRT+GDLGYICMG+KGRYLTEFLIFFAQCGG
Sbjct: 72  ALAGVATYYCMLLLVKCREKLTSQGRSKESRTFGDLGYICMGSKGRYLTEFLIFFAQCGG 131

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHG+A+S YIFLIA VEVVLSWI SLAALAPFSIFADICNAIA
Sbjct: 132 SVAYLVFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIA 191

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF +RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDK+
Sbjct: 192 MGIVVKEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKA 251

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVL QA VGIT VYVLFGFSGYMAYGD+TRDIITLNLP+TWSTKA+QVGLCVGLVFT
Sbjct: 252 AFPKVLAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFT 311

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FPMMLHPINEIVE KLKQS+W+EKI+D++N+FSGK++KVATYISRAIIVLGLA+LASFVP
Sbjct: 312 FPMMLHPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVP 371

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFG+FASLVGST+CALISFVLPAIFHL LMGSSLS GQKVLDSFILI GLLFA YGTYNS
Sbjct: 372 GFGLFASLVGSTVCALISFVLPAIFHLRLMGSSLSFGQKVLDSFILISGLLFAVYGTYNS 431

Query: 421 LVGI 425
           LVGI
Sbjct: 432 LVGI 433

BLAST of Lsi01G016540 vs. ExPASy TrEMBL
Match: A0A6J1GH88 (amino acid transporter ANT1 OS=Cucurbita moschata OX=3662 GN=LOC111453876 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.8e-206
Identity = 377/424 (88.92%), Postives = 402/424 (94.81%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTD VEIPLL+  SS PRTTSTLQALGNIIVSVVGTG+LGLP+AFRIAG+ AGSFGV
Sbjct: 12  MELPTDAVEIPLLH--SSPPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV 71

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
            LA VATYYCMLLLVKCREK T QGRSNESRT+GDLGYICMG+KGRYLTEFLIFFAQCGG
Sbjct: 72  ALAGVATYYCMLLLVKCREKLTSQGRSNESRTFGDLGYICMGSKGRYLTEFLIFFAQCGG 131

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHG+A+S YIFLIA VEVVLSWI SLAALAPFSIFADICNAIA
Sbjct: 132 SVAYLVFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIA 191

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF +RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDK+
Sbjct: 192 MGIVVKEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKA 251

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVL QA VGIT VYVLFGFSGYMAYGD+TRDIITLNLP+TWSTKA+QVGLCVGLVFT
Sbjct: 252 AFPKVLAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFT 311

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FPMMLHPINEIVE KLKQS+W+EKI+D++N+FSGK++KVATYISRAIIVLGLA+LASFVP
Sbjct: 312 FPMMLHPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVP 371

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFG+FASLVGST+CALISFVLPAIFHL LMGSSLS  QKVLDSFILI GLLFA YGTYNS
Sbjct: 372 GFGLFASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAVYGTYNS 431

Query: 421 LVGI 425
           LVGI
Sbjct: 432 LVGI 433

BLAST of Lsi01G016540 vs. ExPASy TrEMBL
Match: A0A6J1DPZ9 (amino acid transporter ANT1 OS=Momordica charantia OX=3673 GN=LOC111023151 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.1e-196
Identity = 357/419 (85.20%), Postives = 385/419 (91.89%), Query Frame = 0

Query: 8   VEIPLLNSSSSSPR---TTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAA 67
           V IPLL+SSSSS     TTSTLQ LGNIIVSV+GTG+LGLP+AFRIAG+AAGS GV+L  
Sbjct: 1   VGIPLLDSSSSSSSSQPTTSTLQTLGNIIVSVIGTGILGLPYAFRIAGWAAGSLGVILTG 60

Query: 68  VATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAY 127
           VATYYCMLLLV CREK   QGRS ESRTYGDLGY+CMGNKGRYLTEFLIFFAQCGGSVAY
Sbjct: 61  VATYYCMLLLVNCREKLASQGRSKESRTYGDLGYMCMGNKGRYLTEFLIFFAQCGGSVAY 120

Query: 128 LVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIV 187
           LVFIGQNLSSVFQGHG+A SSYIFLIAAVE+V+SWIGSLAALAPFSIFADICNAIAMGIV
Sbjct: 121 LVFIGQNLSSVFQGHGLAFSSYIFLIAAVEIVMSWIGSLAALAPFSIFADICNAIAMGIV 180

Query: 188 VKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPK 247
           VKEDIQKAIAGGISF ER  ITSNLRGLPFAGGMAVFCFEGFGMTLALESSMK K+ FP+
Sbjct: 181 VKEDIQKAIAGGISFGERMVITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKYKATFPR 240

Query: 248 VLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMM 307
           VL QA VGITI+YVLFGFSGYMAYGD+TRDIITLNLP+TWST+AVQVGLCVGLVFTFP+M
Sbjct: 241 VLAQAFVGITIMYVLFGFSGYMAYGDETRDIITLNLPNTWSTRAVQVGLCVGLVFTFPIM 300

Query: 308 LHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGV 367
           LHPINEIVEGKLKQSNW +K++D+DN+FS K V +  YISRAIIVLGLA LASF+PGFGV
Sbjct: 301 LHPINEIVEGKLKQSNWLQKVQDSDNVFSRKGVTLVAYISRAIIVLGLATLASFIPGFGV 360

Query: 368 FASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVG 424
           FAS VGST+CALISFVLPAIFHL+LMGSSLS GQKVLDSFILICGL FA YGTYN++VG
Sbjct: 361 FASFVGSTVCALISFVLPAIFHLLLMGSSLSFGQKVLDSFILICGLFFAVYGTYNTIVG 419

BLAST of Lsi01G016540 vs. NCBI nr
Match: XP_038883890.1 (amino acid transporter ANT1 isoform X1 [Benincasa hispida])

HSP 1 Score: 750.4 bits (1936), Expect = 8.7e-213
Identity = 396/425 (93.18%), Postives = 406/425 (95.53%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTDT++IPLLN   SSP TTSTLQAL NIIVSVVGTGVLGLPFAFRIAGYAAGSFGV
Sbjct: 1   MELPTDTLQIPLLN---SSPPTTSTLQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
           LLAAVATYYCMLLLVKCREK T QG+SNES+TYGDLGY+CMGNKGRYLTEFLIFFAQCGG
Sbjct: 61  LLAAVATYYCMLLLVKCREKLTSQGQSNESKTYGDLGYMCMGNKGRYLTEFLIFFAQCGG 120

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHGI LSSYIFLIAAVEVVLSWIGSLAAL PFSIFADICNAIA
Sbjct: 121 SVAYLVFIGQNLSSVFQGHGITLSSYIFLIAAVEVVLSWIGSLAALVPFSIFADICNAIA 180

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMK K+
Sbjct: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKHKA 240

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTK VQVGLCVGLVFT
Sbjct: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKVVQVGLCVGLVFT 300

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDN-DNIFSGKRVKVATYISRAIIVLGLAILASFV 360
           FP+MLHPINEIVEGKLKQ +W+EKIEDN +NIFSGKR   A YISR IIVLGLAILASFV
Sbjct: 301 FPIMLHPINEIVEGKLKQISWFEKIEDNVNNIFSGKRANAAKYISRMIIVLGLAILASFV 360

Query: 361 PGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYN 420
           PGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLD FILICGLLFAAYGTYN
Sbjct: 361 PGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDLFILICGLLFAAYGTYN 420

Query: 421 SLVGI 425
           SLVGI
Sbjct: 421 SLVGI 422

BLAST of Lsi01G016540 vs. NCBI nr
Match: XP_008441964.1 (PREDICTED: amino acid transporter ANT1 [Cucumis melo])

HSP 1 Score: 746.5 bits (1926), Expect = 1.3e-211
Identity = 390/424 (91.98%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTDT+EIPLLNSSS   RTTST QAL NIIVSVVGTGVLGLPFAFRIAGYAAGSFGV
Sbjct: 1   MELPTDTMEIPLLNSSS---RTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
           LL A+ATYYCMLLLVKCREK TLQGRSNES+TYGDLGYICMGNKGRYLTEFLIFFAQCGG
Sbjct: 61  LLVALATYYCMLLLVKCREKLTLQGRSNESKTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHGI LSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA
Sbjct: 121 SVAYLVFIGQNLSSVFQGHGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDK+
Sbjct: 181 MGIVVKEDIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKA 240

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVLGQALVGITIVY+LFGFSGYMAYGDDTRDIITLNLP+TWSTKAVQVGLCVGL+FT
Sbjct: 241 AFPKVLGQALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLIFT 300

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FP+MLHPINEIVEGKL QSNW+EKIEDND IF GKR + ATY+SRA+IVL LAILASFVP
Sbjct: 301 FPIMLHPINEIVEGKLAQSNWFEKIEDNDVIFLGKRAQAATYMSRAVIVLVLAILASFVP 360

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFGVFASLVGSTICALISFVLPAIFHLMLMGSSL L QKVLDS ILICGL+FA YGTYNS
Sbjct: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAVYGTYNS 420

Query: 421 LVGI 425
           L GI
Sbjct: 421 LFGI 421

BLAST of Lsi01G016540 vs. NCBI nr
Match: XP_004147525.1 (amino acid transporter ANT1 [Cucumis sativus] >KGN53957.1 hypothetical protein Csa_021586 [Cucumis sativus])

HSP 1 Score: 737.6 bits (1903), Expect = 5.8e-209
Identity = 385/417 (92.33%), Postives = 399/417 (95.68%), Query Frame = 0

Query: 8   VEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVAT 67
           ++IPLLNSSSSS  TTST QAL NIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLL A+AT
Sbjct: 1   MDIPLLNSSSSSSPTTSTFQALANIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLVALAT 60

Query: 68  YYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF 127
           YYCMLLLVKCREK  LQGRS ES+TYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF
Sbjct: 61  YYCMLLLVKCREKLALQGRSKESQTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVF 120

Query: 128 IGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE 187
           IGQNLSSVFQ +GI LSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE
Sbjct: 121 IGQNLSSVFQSYGIPLSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKE 180

Query: 188 DIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLG 247
           DIQKAIAGGISF ERTAITSNLRGLPFAGGMAVFCFEGFGMTLAL+SSMKDK+AFPKVLG
Sbjct: 181 DIQKAIAGGISFNERTAITSNLRGLPFAGGMAVFCFEGFGMTLALQSSMKDKAAFPKVLG 240

Query: 248 QALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMMLHP 307
           QALVGITIVY+LFGFSGYMAYGDDTRDIITLNLP+TWSTKAVQVGLCVGLVFTFP+MLHP
Sbjct: 241 QALVGITIVYILFGFSGYMAYGDDTRDIITLNLPNTWSTKAVQVGLCVGLVFTFPIMLHP 300

Query: 308 INEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFAS 367
           INEIVEGKL QSNW+EKIEDND+IFSGKR KVATYISR +IVL LAILASFVPGFGVFAS
Sbjct: 301 INEIVEGKLAQSNWFEKIEDNDDIFSGKRAKVATYISRTLIVLVLAILASFVPGFGVFAS 360

Query: 368 LVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI 425
           LVGSTICALISFVLPAIFHLMLMGSSL L QKVLDS ILICGL+FAAYGTYNSLVGI
Sbjct: 361 LVGSTICALISFVLPAIFHLMLMGSSLCLSQKVLDSSILICGLIFAAYGTYNSLVGI 417

BLAST of Lsi01G016540 vs. NCBI nr
Match: XP_022978183.1 (amino acid transporter ANT1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 729.2 bits (1881), Expect = 2.1e-206
Identity = 378/424 (89.15%), Postives = 403/424 (95.05%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTD VEIPLL+  SSSPRTTSTLQALGNIIVSVVGTG+LGLP+AFRIAG+ AGSFGV
Sbjct: 12  MELPTDAVEIPLLH--SSSPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV 71

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
            LA VATYYCMLLLVKCREK T QGRS ESRT+GDLGYICMG+KGRYLTEFLIFFAQCGG
Sbjct: 72  ALAGVATYYCMLLLVKCREKLTSQGRSKESRTFGDLGYICMGSKGRYLTEFLIFFAQCGG 131

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHG+A+S YIFLIA VEVVLSWI SLAALAPFSIFADICNAIA
Sbjct: 132 SVAYLVFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIA 191

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF +RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDK+
Sbjct: 192 MGIVVKEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKA 251

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVL QA VGIT VYVLFGFSGYMAYGD+TRDIITLNLP+TWSTKA+QVGLCVGLVFT
Sbjct: 252 AFPKVLAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFT 311

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FPMMLHPINEIVE KLKQS+W+EKI+D++N+FSGK++KVATYISRAIIVLGLA+LASFVP
Sbjct: 312 FPMMLHPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVP 371

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFG+FASLVGST+CALISFVLPAIFHL LMGSSLS GQKVLDSFILI GLLFA YGTYNS
Sbjct: 372 GFGLFASLVGSTVCALISFVLPAIFHLRLMGSSLSFGQKVLDSFILISGLLFAVYGTYNS 431

Query: 421 LVGI 425
           LVGI
Sbjct: 432 LVGI 433

BLAST of Lsi01G016540 vs. NCBI nr
Match: XP_022950929.1 (amino acid transporter ANT1 [Cucurbita moschata])

HSP 1 Score: 727.2 bits (1876), Expect = 7.9e-206
Identity = 377/424 (88.92%), Postives = 402/424 (94.81%), Query Frame = 0

Query: 1   MELPTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGV 60
           MELPTD VEIPLL+  SS PRTTSTLQALGNIIVSVVGTG+LGLP+AFRIAG+ AGSFGV
Sbjct: 12  MELPTDAVEIPLLH--SSPPRTTSTLQALGNIIVSVVGTGILGLPYAFRIAGWGAGSFGV 71

Query: 61  LLAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGG 120
            LA VATYYCMLLLVKCREK T QGRSNESRT+GDLGYICMG+KGRYLTEFLIFFAQCGG
Sbjct: 72  ALAGVATYYCMLLLVKCREKLTSQGRSNESRTFGDLGYICMGSKGRYLTEFLIFFAQCGG 131

Query: 121 SVAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIA 180
           SVAYLVFIGQNLSSVFQGHG+A+S YIFLIA VEVVLSWI SLAALAPFSIFADICNAIA
Sbjct: 132 SVAYLVFIGQNLSSVFQGHGLAMSYYIFLIAVVEVVLSWIPSLAALAPFSIFADICNAIA 191

Query: 181 MGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKS 240
           MGIVVKEDIQKAIAGGISF +RTAITSN+RGLPFAGGMAVFCFEGFGMTLALESSMKDK+
Sbjct: 192 MGIVVKEDIQKAIAGGISFDKRTAITSNMRGLPFAGGMAVFCFEGFGMTLALESSMKDKA 251

Query: 241 AFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFT 300
           AFPKVL QA VGIT VYVLFGFSGYMAYGD+TRDIITLNLP+TWSTKA+QVGLCVGLVFT
Sbjct: 252 AFPKVLAQAFVGITTVYVLFGFSGYMAYGDETRDIITLNLPNTWSTKAIQVGLCVGLVFT 311

Query: 301 FPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVP 360
           FPMMLHPINEIVE KLKQS+W+EKI+D++N+FSGK++KVATYISRAIIVLGLA+LASFVP
Sbjct: 312 FPMMLHPINEIVEDKLKQSSWFEKIQDSNNVFSGKQLKVATYISRAIIVLGLAMLASFVP 371

Query: 361 GFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNS 420
           GFG+FASLVGST+CALISFVLPAIFHL LMGSSLS  QKVLDSFILI GLLFA YGTYNS
Sbjct: 372 GFGLFASLVGSTVCALISFVLPAIFHLRLMGSSLSFSQKVLDSFILISGLLFAVYGTYNS 431

Query: 421 LVGI 425
           LVGI
Sbjct: 432 LVGI 433

BLAST of Lsi01G016540 vs. TAIR 10
Match: AT3G11900.1 (aromatic and neutral transporter 1 )

HSP 1 Score: 530.4 bits (1365), Expect = 1.3e-150
Identity = 267/423 (63.12%), Postives = 334/423 (78.96%), Query Frame = 0

Query: 5   TDTVEIPLLNSSSSSPR---TTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVL 64
           T    +PL+ S  S       TS LQ LGNIIVS+VGTGVLGLP+AFRIAG+ AGS GV+
Sbjct: 10  TGDSSLPLIKSPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGWLAGSLGVI 69

Query: 65  LAAVATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGS 124
           +   ATYYCMLLL++CR+K   +    ES+TYGDLG+ CMG KGRYLTEFLIF AQCGGS
Sbjct: 70  IVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLIFTAQCGGS 129

Query: 125 VAYLVFIGQNLSSVFQGHGIALSSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAM 184
           VAYLVFIG+NLSS+F  +G+++ S+I ++  +EV LSWI SL+AL+PFSIFADICN IAM
Sbjct: 130 VAYLVFIGRNLSSIFSSYGLSMVSFILILVPIEVGLSWITSLSALSPFSIFADICNIIAM 189

Query: 185 GIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSA 244
             VVKE+++  I G  SF +RTAI+S + GLPFAGG+AVFCFEGF MTLALESSM+++ A
Sbjct: 190 CFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFCFEGFAMTLALESSMREREA 249

Query: 245 FPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTF 304
           FPK+L + L GIT VYVLFGF GYMAYGD T+DIITLNLP+ WS  AVQ+GLCVGL FTF
Sbjct: 250 FPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPNNWSAIAVQIGLCVGLTFTF 309

Query: 305 PMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPG 364
           P+M+HP+NEI+E KLK+ +W +K  +  +  +G   K A + +R ++V+GLA +AS VPG
Sbjct: 310 PIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIFTTRTLLVVGLAAIASLVPG 369

Query: 365 FGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSL 424
           FG FASLVGST+CALISFVLPA +HL L+G SL++  K +D FI+ICGL+FA YGTYN++
Sbjct: 370 FGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSIDVFIVICGLIFAVYGTYNTI 429

BLAST of Lsi01G016540 vs. TAIR 10
Match: AT2G42005.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 235.3 bits (599), Expect = 8.8e-62
Identity = 153/415 (36.87%), Postives = 230/415 (55.42%), Query Frame = 0

Query: 23  TSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVATYYCMLLLVKCREKST 82
           +S  +   N+ +++VG GVLGLP+AF+  G+  G   +   A    +CM+LLV  R K  
Sbjct: 23  SSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLFSVAALINHCMMLLVHIRRK-- 82

Query: 83  LQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVFIGQNLSSVFQG---- 142
             G SN   ++GDLG+   GN GR++ + LI  +Q G  V YL+FIG  L+++ +     
Sbjct: 83  -LGVSNIG-SFGDLGFAACGNLGRFVVDILIILSQAGFCVGYLIFIGNTLANLSKPTKST 142

Query: 143 --------HGIALSS-YIFLIAAVEVVLSWIGSLAALAPFSIFADICNAIAMGIVVKEDI 202
                    G++  S YI+     ++ L+ I +L  LAP SIFAD+ +  AM +V+ EDI
Sbjct: 143 TLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVVDLGAMAVVIVEDI 202

Query: 203 QKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLGQA 262
           +  +      + +      +    +  G+AV+ FEG GM L LES  KDK  F KVL  +
Sbjct: 203 KITVVQ----RPQVVAFGGMSVFFYGMGVAVYAFEGVGMVLPLESETKDKDKFGKVLALS 262

Query: 263 LVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVFTFPMMLHPIN 322
           ++ I ++Y  FG  GYMA+GDDT DIIT NL     +  VQ+GLC+ L FTFP+M++P+ 
Sbjct: 263 MLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVSSLVQLGLCINLFFTFPLMMNPVF 322

Query: 323 EIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFASLV 382
           EIVE +                +SG    +     R ++VL + ++A  VP F  F SLV
Sbjct: 323 EIVERR---------------FWSG----MYCVWLRWLLVLAVTLVALLVPNFADFLSLV 382

Query: 383 GSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI 425
           GS++C  + FVLP++FHLM+    +   Q+ LD  IL+ G++    GT++SL  I
Sbjct: 383 GSSVCCALGFVLPSLFHLMVFKDEMEWKQRALDVGILLLGVILGVSGTWSSLTEI 410

BLAST of Lsi01G016540 vs. TAIR 10
Match: AT4G38250.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-61
Identity = 154/439 (35.08%), Postives = 238/439 (54.21%), Query Frame = 0

Query: 4   PTDTVEIPLLNSSSSSPRTTSTLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLA 63
           P    + PLL      P  +S  +   N+ ++VVG GVLGLP+AF+  G+  G   ++  
Sbjct: 18  PPAREDSPLL---GKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFKRTGWLMGVLLLVSV 77

Query: 64  AVATYYCMLLLVKCREK-STLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSV 123
           +V T++CM+LLV  R K  +     ++  ++GDLG+   G+ GR + +  I  +Q G  V
Sbjct: 78  SVLTHHCMMLLVYTRRKLDSFNAGISKIGSFGDLGFAVCGSLGRIVVDLFIILSQAGFCV 137

Query: 124 AYLVFIGQNLSSVFQGH-----------------GIALSS-YIFLIAAVEVVLSWIGSLA 183
            YL+FIG  L+++                     G++  S YI+     ++ L+ I +L 
Sbjct: 138 GYLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGCFPFQLGLNSIKTLT 197

Query: 184 ALAPFSIFADICNAIAMGIVVKEDIQKAIAGGISFKERTAITS--NLRGLPFAGGMAVFC 243
            LAP SIFADI +  AM +V+ ED        I  K+R  + +   +    +  G+AV+ 
Sbjct: 198 HLAPLSIFADIVDLGAMAVVIVEDSM------IILKQRPDVVAFGGMSLFLYGMGVAVYS 257

Query: 244 FEGFGMTLALESSMKDKSAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPD 303
           FEG GM L LES MKDK  F KVL   +  I+++Y+ FG  GY+A+G+DT DIIT NL  
Sbjct: 258 FEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMDIITANLGA 317

Query: 304 TWSTKAVQVGLCVGLVFTFPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATY 363
              +  VQ+GLC+ L FTFP+M++P+ EIVE +  +  +   +                 
Sbjct: 318 GLVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWL----------------- 377

Query: 364 ISRAIIVLGLAILASFVPGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLD 422
             R ++VL + ++A FVP F  F SLVGS+ C ++ FVLPA+FHL++    +   Q   D
Sbjct: 378 --RWVLVLAVTLVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSD 428

BLAST of Lsi01G016540 vs. TAIR 10
Match: AT5G65990.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.4e-56
Identity = 148/422 (35.07%), Postives = 227/422 (53.79%), Query Frame = 0

Query: 7   TVEIPLLNSSSSSPRTTSTL-QALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAV 66
           T + PLL      PRT S+  +   N+ +++VG GVLGLP+ F+  G+  G   +L  + 
Sbjct: 26  TEDTPLL-----GPRTLSSQPKTFANVFIAIVGAGVLGLPYTFKKTGWLLGLLTLLFVSS 85

Query: 67  ATYYCMLLLVKCREKSTLQGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYL 126
            T++CM+LLV  R K       N   ++GDLG    G  GR + + ++  +Q G  V+YL
Sbjct: 86  LTFFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPAGRLVVDVMLVLSQSGFCVSYL 145

Query: 127 VFIG---QNLSSVFQGHGIAL---SSYIFLIAAVEVVLSWIGSLAALAPFSIFADICNAI 186
           +F+     NL S    H + L   S Y++     ++ L+ I SL  LAP SIFADI +  
Sbjct: 146 IFVATTMANLLSRGTEHILGLDAASIYLWGCFPFQLGLNSIPSLTHLAPLSIFADIVDVA 205

Query: 187 AMGIVVKEDIQKAIAGGISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDK 246
           A  +V+ +D+   +      +    ++    GL    G+AV+ FEG GM L LE   K K
Sbjct: 206 ATLVVMVQDVFIFLKRRPPLRVFGGVSVFFYGL----GVAVYAFEGIGMVLPLELEAKYK 265

Query: 247 SAFPKVLGQALVGITIVYVLFGFSGYMAYGDDTRDIITLNLPDTWSTKAVQVGLCVGLVF 306
             F + LG A+  I+I+Y  FG  GYMAYG++T+DIIT NL     +  VQ+GL + L F
Sbjct: 266 DKFGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIITTNLGTGVVSTLVQLGLAINLFF 325

Query: 307 TFPMMLHPINEIVEGKLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFV 366
           TFP+M+ P+ E+VE +L  S +   +                   R   VL + ++A  V
Sbjct: 326 TFPLMMQPVYEVVERRLCSSRYSVWV-------------------RWATVLVVTLVALLV 385

Query: 367 PGFGVFASLVGSTICALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYN 422
           P F  F SLVGS++C ++ FVLP++FHL    + LS+ + V+D  + + G++ A  GT+ 
Sbjct: 386 PNFADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITGTWT 419

BLAST of Lsi01G016540 vs. TAIR 10
Match: AT2G41190.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 122.5 bits (306), Expect = 8.3e-28
Identity = 108/410 (26.34%), Postives = 184/410 (44.88%), Query Frame = 0

Query: 24  STLQALGNIIVSVVGTGVLGLPFAFRIAGYAAGSFGVLLAAVATYYCMLLLVKCREKSTL 83
           S +Q + N I  + G G+L  P+  + AG+A+    +LL AV   Y   L+  C E  T 
Sbjct: 148 SVIQTIFNAINVMAGVGLLSTPYTVKEAGWASMVI-LLLFAVICCYTATLMKDCFENKT- 207

Query: 84  QGRSNESRTYGDLGYICMGNKGRYLTEFLIFFAQCGGSVAYLVFIGQNLSSVFQGHGIAL 143
                   TY D+G    G  GR L   L++       V +++  G NL+ +F G  + L
Sbjct: 208 -----GIITYPDIGEAAFGKYGRILICMLLYTELYSYCVEFIILEGDNLTGLFPGTSLDL 267

Query: 144 ------SSYIFLIAAVEVVLS--WIGSLAALAPFSIFADICNAIAMGIVVKEDIQKAIAG 203
                 S ++F I    +VL   W+  L  ++    +      IA  ++          G
Sbjct: 268 LGFRLDSKHLFGILTALIVLPTVWLKDLRIIS----YLSAGGVIATALIAVSVFFLGTTG 327

Query: 204 GISFKERTAITSNLRGLPFAGGMAVFCFEGFGMTLALESSMKDKSAFPKVLGQALVGITI 263
           GI F   T       G+PFA G+  FC+ G  +   +  SM DK+ F K +    +   +
Sbjct: 328 GIGF-HHTGQAVKWNGIPFAIGIYGFCYSGHSVFPNIYQSMADKTKFNKAVITCFIICVL 387

Query: 264 VYVLFGFSGYMAYGDDTRDIITLNLP-DTWSTKAVQVGLCVGLVFTFPMMLHPINEIVEG 323
           +Y      GY+ +G+ T   ITLN+P D + +K  Q    V     + ++++P+   +E 
Sbjct: 388 LYGGVAIMGYLMFGEATLSQITLNMPQDQFFSKVAQWTTVVSPFTKYALLMNPLARSIEE 447

Query: 324 KLKQSNWYEKIEDNDNIFSGKRVKVATYISRAIIVLGLAILASFVPGFGVFASLVGSTIC 383
            L      E++ +N   F          + R  +V      A  +P FG+  +L+GS + 
Sbjct: 448 LLP-----ERMSENIWCF---------LLLRTALVASSVCSAFLIPFFGLMMALIGSLLS 507

Query: 384 ALISFVLPAIFHLMLMGSSLSLGQKVLDSFILICGLLFAAYGTYNSLVGI 425
            L++ ++PA+  + +MG+  +  Q +L S I+  G++    GTY+S+  I
Sbjct: 508 ILVAIIMPALCFIKIMGNKATRTQMILSSIIVAIGVVSGTLGTYSSVAKI 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SF091.9e-14963.12Amino acid transporter ANT1 OS=Arabidopsis thaliana OX=3702 GN=ANT1 PE=1 SV=1[more]
F4ILY91.2e-6036.87Amino acid transporter AVT3B OS=Arabidopsis thaliana OX=3702 GN=AVT3B PE=2 SV=1[more]
Q9SVG03.6e-6035.08Amino acid transporter AVT3C OS=Arabidopsis thaliana OX=3702 GN=AVT3C PE=1 SV=1[more]
Q9FKY32.0e-5535.07Amino acid transporter AVT3A OS=Arabidopsis thaliana OX=3702 GN=AVT3A PE=1 SV=1[more]
Q495N29.2e-3227.63Proton-coupled amino acid transporter 3 OS=Homo sapiens OX=9606 GN=SLC36A3 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S3B5C46.1e-21291.98amino acid transporter ANT1 OS=Cucumis melo OX=3656 GN=LOC103485964 PE=3 SV=1[more]
A0A0A0L0262.8e-20992.33Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G193760 PE... [more]
A0A6J1ISA21.0e-20689.15amino acid transporter ANT1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114782... [more]
A0A6J1GH883.8e-20688.92amino acid transporter ANT1 OS=Cucurbita moschata OX=3662 GN=LOC111453876 PE=3 S... [more]
A0A6J1DPZ92.1e-19685.20amino acid transporter ANT1 OS=Momordica charantia OX=3673 GN=LOC111023151 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_038883890.18.7e-21393.18amino acid transporter ANT1 isoform X1 [Benincasa hispida][more]
XP_008441964.11.3e-21191.98PREDICTED: amino acid transporter ANT1 [Cucumis melo][more]
XP_004147525.15.8e-20992.33amino acid transporter ANT1 [Cucumis sativus] >KGN53957.1 hypothetical protein C... [more]
XP_022978183.12.1e-20689.15amino acid transporter ANT1 isoform X1 [Cucurbita maxima][more]
XP_022950929.17.9e-20688.92amino acid transporter ANT1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G11900.11.3e-15063.12aromatic and neutral transporter 1 [more]
AT2G42005.18.8e-6236.87Transmembrane amino acid transporter family protein [more]
AT4G38250.12.6e-6135.08Transmembrane amino acid transporter family protein [more]
AT5G65990.11.4e-5635.07Transmembrane amino acid transporter family protein [more]
AT2G41190.18.3e-2826.34Transmembrane amino acid transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 423..424
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR22950:SF657BNAA05G27230D PROTEINcoord: 8..423
NoneNo IPR availablePANTHERPTHR22950AMINO ACID TRANSPORTERcoord: 8..423
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 21..420
e-value: 2.0E-92
score: 310.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G016540.1Lsi01G016540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:0015801 aromatic amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0015804 neutral amino acid transport
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015173 aromatic amino acid transmembrane transporter activity
molecular_function GO:0015175 neutral amino acid transmembrane transporter activity
molecular_function GO:0015293 symporter activity