Lsi01G016400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G016400
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase Praja-2
Location	chr01: 14824665 .. 14828557 (+)
RNA-Seq Expression	Lsi01G016400
Synteny	Lsi01G016400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCAGCCTGAGTGCAGTGGTTGAAGCCTGCTGAGGCTGAGTGTGGCCCATAACACAAGAGCCAACAGTCAAACGGACCCATTTCAAACACTTTATTTTGTGTAATCATCAGACCAACAAATCCAACTTTTTCTCCACAAAAACAAACCAATTAGATCAAACCATCAACACCCAGTTACAGAATTGAATCATTCAACCCCACTGTTCAATTTTTTTGGTTGAATCTTCTTTCTTTTCAGAAGAATGTTGGTGGGTCATTGTCAATCACACCGAAGCGCGCTTTGATTAGGGAAGTTCTCGTCTTCCATCCTCCAATGGCGGCTGATCCTCCTTCAAACCGCTTTTCACGAAGAACCCCATTGACCTCTCCGCTTGAAAATCCCACTGGATTTCGTTTCCCCTTCTGATTTCTGTTCATCTTCGTACCCATTTTCACCCACCCACTACAGATGTTTCTGAACAGCGTTCTTCAATTATTTGTCTCTCTTCATTTTCTTTCCTCAACCGCTTTGCCCTTTTCGTTGATTGTCTTATTTTCTTCGTTTCACTCAAGTTTTCGGTTCGTTTTGTTCGATTGGGTGAGCTCGAGCCGAATGTTGGAGTTTTGAAGGACTGGAAGCAAATTGACTGGTAATAATCGCAACCCATCTTCGTCTTCTCTGATCTCTTTGATGCATTTTTTGGTTTTCCCTAATTGTATGTAGTACCGTTTTTGTGTTTCTTTGCGCCAGTCATTTGATTTCTGATTTGGGATTTTTGGTCTCTGGCTTGCTAATTTGATACTTTAGGAAGTACGACTGTAGTGAGAATCATCGCATTAAGTGGTTGCATCGTTTCTCCTTGAAGAAACCATTAGTTCCCTTTTGCCTTGTTTACTGATTGAACTTCTGTAACTTCTCTTGAACCCTTGAAATCCATGTTAGCAACTGTTGAACACTGGTTATTGAGATTGTTGTTGGAGGGTTTTAGTATCAAAATTGGGGATGGAACTTGTGGCTGACATTGGTTCTTTATTAACTCTCATCATGGTGTGAGAAGATTTCCCTCAATTTACGGTGCCTCCTACTTTAGGGAGTGTTTGTAGCACTGAAGCACTGAGTTGAGTTATGAAGTCTGAAGTTAAAAAGATTGTGTTTGGGGTGTAGAGTTATGAAGATAAAGTCCTCGATAAATGTGCAAAAATGAGTAATGATCAAATTTTTCATCACTGGGGAGACAACGGAGGGAGACTTATACCATGCTGTTGATGAAAGGACAGTTTAGTTTAGATTGAGATAAGAATATGGTTTAGTTTGAAACAAAGAGAGTGAAGTGAGGTTACGAATGAAGGGGTAAAGGTGTTATCTAATGCTGCTTATCCGGGTTCTTTTCTCTTTTAAATATTTTCACATTTAGGGTTGTTGAGGGTAGGGAGTAGTTGTCAGTCTACTCGAGATAACACTGATAAGGAGCAAAAATCGTGACAGTTTTCTTACTGAAGTAATAGATTAATGAAACTAATCAAATGAGAATGAAAATATAAGACAACATCGTCAGGAACTGAGTACATGGCCACAAGTGCAGAAAACTATGACAAGTCAGCGTCTAATTTCTTAGTAACAAATTCTTATGGCATATTGTTGAACCAGTGTGGTCAGTCCCTCAAGGCGTTTGATTGTTGTCTCTTATTTGAAAGGGTTGTTTGTCTACATAATTTGCATTATGTGTTCCATCTTTGACTGTTGACCTTACTTGAACAAGATCTGATATATATGTTGTACAACTGTGTCATTGAGTTCTAAGGAGGCAAGTACTCCATCTTTGACTGGACTTCAACTTAGTTAAATCTGATTTGGTCTCTCCTTTAGGTTATTATTTCACCCTTTTTTGAACTTCATGTTGGTTTTCCACTGTTCATTATTTGTGCTTTCTGGTCTTATTTCAGATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCACTGCTCATGTCATCATGAACAGCAACCAACAAATCGACGAACATAGTCCAATTCAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTAGTACCTGACAATGATGCCACTGGTGAACCTGAAGCCATTGATATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATTGAAACCCCTGTACGGGATTCTTATCACAGGACAACGCCCAGAGGAAGAAGAACTAGGCATGGTAGTTCTCAGTCTTCTGAAAATCTTTTTTCACAGGAATTCACCCATATGATTAACATGGTGAGGCAAAGGCAATCCAGCATCTCCAGCCACGGAGATCGATATGCAGATGGTTCGAATGCTGCTGATTTCACGCAGCACTCGAGTTCCCATACAACCCCTAATCAATCTAGACGATGGCGGCAAGCATATTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGGAGAGAGTGAATCGAATTTCAGTTTTGGGCCTTATAGACATTCGTATGGTGATAGTGATGCTGTTTCCTATAGAACTTACGGGGGTGATTCTGATGCTTCCTTTGATGGACATAGTTTCTTGGATACAGACATTTTTGTTCTGCCAGATGAGGGATCTGATGTCGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCAAGTGATGAGGAGGAGGATCGTGGTGAATTTACGGAAGCTGACACAGAGCAAGAAGTAGAATCTTTTGAAGCTAGACCTCAACTTCAAAATTTTCATGCTTCGAGTGCTCCTGGGAGAGGTAACTCGAGCAATTGGGACGAACGGCTTTCTTCACCGGAATATGAGGGTTCAGCTCTCGGGCAAATCAGGAGGAACAGGCGATTTTATTCAATCACCAACTATGCACAATCAGAGTTACTATCTTATATTGGGGATTCTGGTGACTATCTTGACAGACAAGGTTTCGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCACCTCCTGCAGCTGTTTCTTTTGTGAAAAATCTCCCACGTTTGGTGATTTCTAAGGAACATTTGAAGCATGACAGTATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGTGTTGAAGTGAATCAACTTCCTTGTCTTCATCTCTACCACCCTTCCTGCATCTCGCCGTGGTTGAGTGCCCGAAATTCATGCCCCCTTTGTCGTTGTGAACTTCCAACTGATGATCGAGATTATGAAGAGGTAAAGCGAAGTAGTACCAATACTACAGAGATCCATGGACTTCAGCCTCGAGTAGTTGGACTTGAAAATCCCAGTGACTTGAATGGAGTTGGAGCGAATCCAGAGTTTGAAACAAGCCAAGGAGAGGATCAGAGGAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGAGGAGGTAGACGACGTTGGCTGTTCGTCGCTGCTCCAATAGTTGGTCTCGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGCTCCGGGAGGCCGTTTTACAGACAGAAGGCAGCTCGATGCTCACATTGCAGATTCGTCAAACCAAAGAGAGAACAGAACCAGAAGATGGTGGTAAATCGTCTAGCGATTGCCAGGTTTAGTATGAAAAATCGATCTAACACGAGCGTTCTTGTTTTGTTGATTTTCCAGGATTGAAATCTAAGGTTTTCAGCATGTAATTCAGTAAAATTCCAGTTTTCCTAAGTGTTGAAGTAGAAGAGTTGATATAAACCCACAAAGCAATTTGTGCCTTGCTTGTGGACACAATGGGGCAGTAATAAACGGGCCAATTGGTTCGATTGTGAATATATATATACATACATCTTATAATTTATGTAAATAAAACTAAATATTTGAATCCAA

mRNA sequence

AGCAGCCTGAGTGCAGTGGTTGAAGCCTGCTGAGGCTGAGTGTGGCCCATAACACAAGAGCCAACAGTCAAACGGACCCATTTCAAACACTTTATTTTGTGTAATCATCAGACCAACAAATCCAACTTTTTCTCCACAAAAACAAACCAATTAGATCAAACCATCAACACCCAGTTACAGAATTGAATCATTCAACCCCACTGTTCAATTTTTTTGGTTGAATCTTCTTTCTTTTCAGAAGAATGTTGGTGGGTCATTGTCAATCACACCGAAGCGCGCTTTGATTAGGGAAGTTCTCGTCTTCCATCCTCCAATGGCGGCTGATCCTCCTTCAAACCGCTTTTCACGAAGAACCCCATTGACCTCTCCGCTTGAAAATCCCACTGGATTTCGTTTCCCCTTCTGATTTCTGTTCATCTTCGTACCCATTTTCACCCACCCACTACAGATGTTTCTGAACAGCGTTCTTCAATTATTTGTCTCTCTTCATTTTCTTTCCTCAACCGCTTTGCCCTTTTCGTTGATTGTCTTATTTTCTTCGTTTCACTCAAGTTTTCGGTTCGTTTTGTTCGATTGGGTGAGCTCGAGCCGAATGTTGGAGTTTTGAAGGACTGGAAGCAAATTGACTGATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCACTGCTCATGTCATCATGAACAGCAACCAACAAATCGACGAACATAGTCCAATTCAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTAGTACCTGACAATGATGCCACTGGTGAACCTGAAGCCATTGATATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATTGAAACCCCTGTACGGGATTCTTATCACAGGACAACGCCCAGAGGAAGAAGAACTAGGCATGGTAGTTCTCAGTCTTCTGAAAATCTTTTTTCACAGGAATTCACCCATATGATTAACATGGTGAGGCAAAGGCAATCCAGCATCTCCAGCCACGGAGATCGATATGCAGATGGTTCGAATGCTGCTGATTTCACGCAGCACTCGAGTTCCCATACAACCCCTAATCAATCTAGACGATGGCGGCAAGCATATTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGGAGAGAGTGAATCGAATTTCAGTTTTGGGCCTTATAGACATTCGTATGGTGATAGTGATGCTGTTTCCTATAGAACTTACGGGGGTGATTCTGATGCTTCCTTTGATGGACATAGTTTCTTGGATACAGACATTTTTGTTCTGCCAGATGAGGGATCTGATGTCGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCAAGTGATGAGGAGGAGGATCGTGGTGAATTTACGGAAGCTGACACAGAGCAAGAAGTAGAATCTTTTGAAGCTAGACCTCAACTTCAAAATTTTCATGCTTCGAGTGCTCCTGGGAGAGGTAACTCGAGCAATTGGGACGAACGGCTTTCTTCACCGGAATATGAGGGTTCAGCTCTCGGGCAAATCAGGAGGAACAGGCGATTTTATTCAATCACCAACTATGCACAATCAGAGTTACTATCTTATATTGGGGATTCTGGTGACTATCTTGACAGACAAGGTTTCGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCACCTCCTGCAGCTGTTTCTTTTGTGAAAAATCTCCCACGTTTGGTGATTTCTAAGGAACATTTGAAGCATGACAGTATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGTGTTGAAGTGAATCAACTTCCTTGTCTTCATCTCTACCACCCTTCCTGCATCTCGCCGTGGTTGAGTGCCCGAAATTCATGCCCCCTTTGTCGTTGTGAACTTCCAACTGATGATCGAGATTATGAAGAGGTAAAGCGAAGTAGTACCAATACTACAGAGATCCATGGACTTCAGCCTCGAGTAGTTGGACTTGAAAATCCCAGTGACTTGAATGGAGTTGGAGCGAATCCAGAGTTTGAAACAAGCCAAGGAGAGGATCAGAGGAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGAGGAGGTAGACGACGTTGGCTGTTCGTCGCTGCTCCAATAGTTGGTCTCGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGCTCCGGGAGGCCGTTTTACAGACAGAAGGCAGCTCGATGCTCACATTGCAGATTCGTCAAACCAAAGAGAGAACAGAACCAGAAGATGGTGGTAAATCGTCTAGCGATTGCCAGGTTTAGTATGAAAAATCGATCTAACACGAGCGTTCTTGTTTTGTTGATTTTCCAGGATTGAAATCTAAGGTTTTCAGCATGTAATTCAGTAAAATTCCAGTTTTCCTAAGTGTTGAAGTAGAAGAGTTGATATAAACCCACAAAGCAATTTGTGCCTTGCTTGTGGACACAATGGGGCAGTAATAAACGGGCCAATTGGTTCGATTGTGAATATATATATACATACATCTTATAATTTATGTAAATAAAACTAAATATTTGAATCCAA

Coding sequence (CDS)

ATGGACATTGACTTGCAAAGAATCTTGGAAGAGTTTCCTCAATCCACTGCTCATGTCATCATGAACAGCAACCAACAAATCGACGAACATAGTCCAATTCAGCTGCCAACTTGTGCATTTTGCCAACGAGTCCTAGTACCTGACAATGATGCCACTGGTGAACCTGAAGCCATTGATATATGTGGCGATTGTAAATTTTTGTTACTCGAAGATATTGAAACCCCTGTACGGGATTCTTATCACAGGACAACGCCCAGAGGAAGAAGAACTAGGCATGGTAGTTCTCAGTCTTCTGAAAATCTTTTTTCACAGGAATTCACCCATATGATTAACATGGTGAGGCAAAGGCAATCCAGCATCTCCAGCCACGGAGATCGATATGCAGATGGTTCGAATGCTGCTGATTTCACGCAGCACTCGAGTTCCCATACAACCCCTAATCAATCTAGACGATGGCGGCAAGCATATTCTGATTCTGAAAATGATGGTTTAGATAGTTTTGACTCTATGTTTGGAGAGAGTGAATCGAATTTCAGTTTTGGGCCTTATAGACATTCGTATGGTGATAGTGATGCTGTTTCCTATAGAACTTACGGGGGTGATTCTGATGCTTCCTTTGATGGACATAGTTTCTTGGATACAGACATTTTTGTTCTGCCAGATGAGGGATCTGATGTCGATACTGATACAGACATTGATCCCATGCATGCTGGTGTCGGCCATTGGGACTCAAGTGATGAGGAGGAGGATCGTGGTGAATTTACGGAAGCTGACACAGAGCAAGAAGTAGAATCTTTTGAAGCTAGACCTCAACTTCAAAATTTTCATGCTTCGAGTGCTCCTGGGAGAGGTAACTCGAGCAATTGGGACGAACGGCTTTCTTCACCGGAATATGAGGGTTCAGCTCTCGGGCAAATCAGGAGGAACAGGCGATTTTATTCAATCACCAACTATGCACAATCAGAGTTACTATCTTATATTGGGGATTCTGGTGACTATCTTGACAGACAAGGTTTCGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCACCTCCTGCAGCTGTTTCTTTTGTGAAAAATCTCCCACGTTTGGTGATTTCTAAGGAACATTTGAAGCATGACAGTATATCCTGTGCAATTTGCAAGGATTTCTTACTTGTAGGTGTTGAAGTGAATCAACTTCCTTGTCTTCATCTCTACCACCCTTCCTGCATCTCGCCGTGGTTGAGTGCCCGAAATTCATGCCCCCTTTGTCGTTGTGAACTTCCAACTGATGATCGAGATTATGAAGAGGTAAAGCGAAGTAGTACCAATACTACAGAGATCCATGGACTTCAGCCTCGAGTAGTTGGACTTGAAAATCCCAGTGACTTGAATGGAGTTGGAGCGAATCCAGAGTTTGAAACAAGCCAAGGAGAGGATCAGAGGAACATAGACTCTGCAGTTAATAATAGTAACAATTCAGGCAGAGGAGGTAGACGACGTTGGCTGTTCGTCGCTGCTCCAATAGTTGGTCTCGTAGGTATAGCCCTGATGTTATGGTTTGGCAATCCTTTATGTGATCAAAGAGCTCCGGGAGGCCGTTTTACAGACAGAAGGCAGCTCGATGCTCACATTGCAGATTCGTCAAACCAAAGAGAGAACAGAACCAGAAGATGGTGGTAA

Protein sequence

MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDICGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSISSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSFGPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVGHWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEGSALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGEDQRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQLDAHIADSSNQRENRTRRWW

Homology

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BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.6e-26
Identity = 54/116 (46.55%), Postives = 72/116 (62.07%), Query Frame = 0


Query: 331 GDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDF 390
           GDY    G E+L++Q+AE   +R G PPA+ S ++ LP + I+K +L  +   CA+C D 
Sbjct: 171 GDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDD 230

Query: 391 LLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTT 447
              G E  Q+PC HLYH  C+ PWL   NSCP+CR ELPTDD DYE   R +  T+
Sbjct: 231 FEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 286

[Close]

BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 3.4e-26
Identity = 53/123 (43.09%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           G+ GDY    G E+L++Q+AE   +R G PPA+ S +  LP + ++K+ LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTTE 447
            D    G +V Q+PC H++H  C+ PWL   NSCP+CR ELPTDD DYE   + S  + +
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGD 279

Query: 448 IHG 451
             G
Sbjct: 280 GQG 282

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BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.1e-23
Identity = 56/139 (40.29%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           G++GDY    G EEL EQ++  T +RRG PPA  S +  LP + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELP------------TDDRDY 447
           KD   +G E  Q+PC H+YH  CI PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 448 EEVKRSSTNTTEIHGLQPR 455
                SS++ +  +G + R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

[Close]

BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.6e-23
Identity = 79/228 (34.65%), Postives = 111/228 (48.68%), Query Frame = 0


Query: 224 SDVDTDTDIDPMHAGVGHWDSSDEEEDRGE-FTEADTEQEVESFEARPQLQNFHASSAPG 283
           +++D D D D         D  +EEED  E  T  D E E +    R +       S  G
Sbjct: 115 NEIDDDEDED-------EDDGDEEEEDEEENLTVNDEEDEEDDLRRRNRFPLTTTQSRTG 174

Query: 284 RGNSSNWDERLSSPEYEGSALGQIRRNRRFYSITNYAQSELLSYIGDSGDYL-DRQGFEE 343
           R    +W E L   E + S   ++  +R               Y G+  DY+ D  G+E 
Sbjct: 175 RNRILDWAEILMGIE-DNSIEFRMESDR---------------YAGNPADYIDDAAGYEA 234

Query: 344 LLEQIAETT----SSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEV 403
           LL+ +AE        RRGAPPAA S ++ L    +S    +   + CA+CKD +++G   
Sbjct: 235 LLQNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMV-CAVCKDGMVMGETG 294

Query: 404 NQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNT 446
            +LPC H YH  CI PWL  RNSCP+CR +L TDD +YEE ++  T+T
Sbjct: 295 KKLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTST 318

[Close]

BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 7.4e-21
Identity = 51/121 (42.15%), Postives = 69/121 (57.02%), Query Frame = 0


Query: 329 DSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICK 388
           ++GDY    G EEL+EQ++  T   RG PPA  S +  LP + I+++HLK     C +CK
Sbjct: 132 NAGDYFFGPGLEELIEQLSSGT-HHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCK 191

Query: 389 DFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPT--DDRDYEEVKRSSTNTT 448
           D   +  E  Q+PC H+YH  CI PWL   NSCP+CR ELP+       +  +  STN  
Sbjct: 192 DEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGR 251

[Close]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL
Match: A0A0A0L2Z4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 PE=4 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 7.4e-282
Identity = 492/562 (87.54%), Postives = 512/562 (91.10%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQS  HVIMN+NQ++DEHSPIQLPTCAFCQRVLVPD+DA  EPE IDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHR TPRGRR    SS+S ENLFSQEFTHMINMVRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA DF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASF-DGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGV 240
           GPYRHSYGDSD VS RTYGG+SD S  DGHSFLDTD+F+LP EGS  DTDTDIDPM AGV
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYE 300
           GHWDSSDEE+D GEFTEA+TEQEV S EARPQLQNFH SSA  RGNSSNW+E+LSSPEYE
Sbjct: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300

Query: 301 GSALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAE-TTSSRRGAPP 360
           GS  G IRRNRRFYSITNYAQSELLSY+GDSGDYLDRQGFEELLEQIAE TTSSRRGAPP
Sbjct: 301 GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPP 360

Query: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSAR 420
           AAVSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHPSCI PWLSAR
Sbjct: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSAR 420

Query: 421 NSCPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQG 480
           NSCPLCR ELPTDDRDYEEVK+SS N   +HGLQPRVVGLENPS LNGVGANPEFETSQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQE 480

Query: 481 EDQRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDR 540
           EDQRNIDSAV +SNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQR  GG+ TD 
Sbjct: 481 EDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDG 540

Query: 541 RQLDAHIADSSNQRENRTRRWW 561
           RQLDAH A+SSNQRENR+RRWW
Sbjct: 541 RQLDAHSAESSNQRENRSRRWW 560

[Close]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL
Match: A0A5D3C795 (E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold287G00690 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.1e-280
Identity = 490/560 (87.50%), Postives = 510/560 (91.07%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQS  HVIMNSNQ++DEHSPIQLPTCAFCQRVL  D+DAT EPE IDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHR TPRGRRT   SS+S ENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA DF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VS RTYGGDSDAS DGHSFLDTDIF+LP EGS  DTDTDIDPM AG+G
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIG 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           +WDSSDEE+D GEFTEA+TEQE+ S EARPQLQN + SSA  RGNSSNW E+LSSPEYEG
Sbjct: 241 NWDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           S  G IRRNRRFYSITNYAQSELLSY+GDSGDYLDRQGFE+LLEQIAETTSSRRGAPPAA
Sbjct: 301 STPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHP+CI PWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEEVKRSS NT   H LQP VVGLENPS LNGVGAN EFE+SQ ED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
            RNIDSAV +SNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQR PGG+ TD RQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           LDA  A+SSNQRENRTRRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL
Match: A0A1S3CIY7 (uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.1e-280
Identity = 490/560 (87.50%), Postives = 510/560 (91.07%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQS  HVIMNSNQ++DEHSPIQLPTCAFCQRVL  D+DAT EPE IDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHR TPRGRRT   SS+S ENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA DF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VS RTYGGDSDAS DGHSFLDTDIF+LP EGS  DTDTDIDPM AG+G
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIG 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           +WDSSDEE+D GEFTEA+TEQE+ S EARPQLQN + SSA  RGNSSNW E+LSSPEYEG
Sbjct: 241 NWDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           S  G IRRNRRFYSITNYAQSELLSY+GDSGDYLDRQGFE+LLEQIAETTSSRRGAPPAA
Sbjct: 301 STPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHP+CI PWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEEVKRSS NT   H LQP VVGLENPS LNGVGAN EFE+SQ ED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
            RNIDSAV +SNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQR PGG+ TD RQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           LDA  A+SSNQRENRTRRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL
Match: A0A6J1GDT0 (uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.7e-252
Identity = 450/560 (80.36%), Postives = 476/560 (85.00%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDID QRILEEFP STA +IM++NQQ++E  P+Q  TCAFC+ VL PDNDAT E EAI I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HRTTPRGRRTRHGSS+S+ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           S H DRY DGSNAADF QHSSSHTTPNQSRRWRQ YSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS DGHSFLDTD FVLPDEGS VDTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           HWDSSDEE+DRGE TEADTE +         LQ+FH SSA GRGN  NW+E LSS EYEG
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           SA  QIRRN RFYSITNYA SE +SY+GDSGDYLDRQ FEELLEQIAETTSSRRGAPPAA
Sbjct: 301 SAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           +SFVKNL RLVISKEHLKHDSISCAICKDF LVGVEVNQLPCLHLYHP CI PWL+ARNS
Sbjct: 361 ISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEE K+SSTN  EI  LQ +V  LE+ SDLN VGANPEF+TSQGE+
Sbjct: 421 CPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQGEE 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
             NID AV+NS NSGR GRR WLFVAAP+VGLVGIALM W GNP       GGR TDRRQ
Sbjct: 481 LSNIDPAVSNSYNSGR-GRRSWLFVAAPVVGLVGIALMFWVGNP------SGGRITDRRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           LD   ADSSNQRENRTRRWW
Sbjct: 541 LDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL
Match: A0A6J1GEL6 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 8.2e-249
Identity = 451/585 (77.09%), Postives = 477/585 (81.54%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDID QRILEEFP STA +IM++NQQ++E  P+Q  TCAFC+ VL PDNDAT E EAI I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HRTTPRGRRTRHGSS+S+ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           S H DRY DGSNAADF QHSSSHTTPNQSRRWRQ YSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS DGHSFLDTD FVLPDEGS VDTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           HWDSSDEE+DRGE TEADTE +         LQ+FH SSA GRGN  NW+E LSS EYEG
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYEG 300

Query: 301 SAL-------------------------GQIRRNRRFYSITNYAQSELLSYIGDSGDYLD 360
           SA                          GQIRRN RFYSITNYA SE +SY+GDSGDYLD
Sbjct: 301 SARDSITNNAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAHSESVSYVGDSGDYLD 360

Query: 361 RQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGV 420
           RQ FEELLEQIAETTSSRRGAPPAA+SFVKNL RLVISKEHLKHDSISCAICKDF LVGV
Sbjct: 361 RQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGV 420

Query: 421 EVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRV 480
           EVNQLPCLHLYHP CI PWL+ARNSCPLCR ELPTDDRDYEE K+SSTN  EI  LQ +V
Sbjct: 421 EVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQV 480

Query: 481 VGLENPSDLNGVGANPEFETSQGEDQRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGI 540
             LE+ SDLN VGANPEF+TSQGE+  NID AV+NS NSGR GRR WLFVAAP+VGLVGI
Sbjct: 481 TRLESRSDLNEVGANPEFDTSQGEELSNIDPAVSNSYNSGR-GRRSWLFVAAPVVGLVGI 540

Query: 541 ALMLWFGNPLCDQRAPGGRFTDRRQLDAHIADSSNQRENRTRRWW 561
           ALM W GNP       GGR TDRRQLD   ADSSNQRENRTRRWW
Sbjct: 541 ALMFWVGNP------SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

[Close]

BLAST of Lsi01G016400 vs. NCBI nr
Match: XP_038882118.1 (E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.7e-299
Identity = 515/560 (91.96%), Postives = 533/560 (95.18%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQSTAHVIMNSNQ++DEHSPIQLP+CAFCQRVLVPDNDATGEPEAID+
Sbjct: 1   MDIDLQRILEEFPQSTAHVIMNSNQRVDEHSPIQLPSCAFCQRVLVPDNDATGEPEAIDV 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLED+ETPVRDSYHRTT RGRRTR+GS +S+ENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDVETPVRDSYHRTTSRGRRTRYGSVESAENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNAADF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSDAVSYRTYGGDSDAS DGHSFLDTDIFVLP EGSDVDTDTDIDPM AGVG
Sbjct: 181 GPYRHSYGDSDAVSYRTYGGDSDASLDGHSFLDTDIFVLPGEGSDVDTDTDIDPMRAGVG 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           HWDSSDEE+D GEFTEADTEQEVES EARPQLQNFH SSAPGRG SSNW+E+ SSPEYEG
Sbjct: 241 HWDSSDEEDDHGEFTEADTEQEVESSEARPQLQNFHVSSAPGRGISSNWNEQFSSPEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           SA GQIRRNRRFYSITNYAQSELLSYIGDSG+YLDRQ FEELLEQIAETTSSRRGAPPAA
Sbjct: 301 SAPGQIRRNRRFYSITNYAQSELLSYIGDSGEYLDRQVFEELLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           +S VKNLPRLVISKEHLKHD+ISCAICKDFLLVGVEVNQLPCLHLYHPSCI PWLSARNS
Sbjct: 361 ISIVKNLPRLVISKEHLKHDTISCAICKDFLLVGVEVNQLPCLHLYHPSCILPWLSARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEEVKRSS NT EIHGLQPRVV LENP DLN VGA+P FETSQGED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAEIHGLQPRVVSLENPRDLN-VGASPAFETSQGED 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
           Q NIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQR  GG+FTDRRQ
Sbjct: 481 QTNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQFTDRRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           L+A IADSSNQRENRTRRWW
Sbjct: 541 LNARIADSSNQRENRTRRWW 559

[Close]

BLAST of Lsi01G016400 vs. NCBI nr
Match: XP_004142724.1 (E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical protein Csa_012302 [Cucumis sativus])

HSP 1 Score: 979.2 bits (2530), Expect = 1.5e-281
Identity = 492/562 (87.54%), Postives = 512/562 (91.10%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQS  HVIMN+NQ++DEHSPIQLPTCAFCQRVLVPD+DA  EPE IDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHR TPRGRR    SS+S ENLFSQEFTHMINMVRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA DF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASF-DGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGV 240
           GPYRHSYGDSD VS RTYGG+SD S  DGHSFLDTD+F+LP EGS  DTDTDIDPM AGV
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGS--DTDTDIDPMRAGV 240

Query: 241 GHWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYE 300
           GHWDSSDEE+D GEFTEA+TEQEV S EARPQLQNFH SSA  RGNSSNW+E+LSSPEYE
Sbjct: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300

Query: 301 GSALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAE-TTSSRRGAPP 360
           GS  G IRRNRRFYSITNYAQSELLSY+GDSGDYLDRQGFEELLEQIAE TTSSRRGAPP
Sbjct: 301 GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPP 360

Query: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSAR 420
           AAVSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHPSCI PWLSAR
Sbjct: 361 AAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSAR 420

Query: 421 NSCPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQG 480
           NSCPLCR ELPTDDRDYEEVK+SS N   +HGLQPRVVGLENPS LNGVGANPEFETSQ 
Sbjct: 421 NSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQE 480

Query: 481 EDQRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDR 540
           EDQRNIDSAV +SNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQR  GG+ TD 
Sbjct: 481 EDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDG 540

Query: 541 RQLDAHIADSSNQRENRTRRWW 561
           RQLDAH A+SSNQRENR+RRWW
Sbjct: 541 RQLDAHSAESSNQRENRSRRWW 560

[Close]

BLAST of Lsi01G016400 vs. NCBI nr
Match: XP_008463301.1 (PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ubiquitin-protein ligase Praja-2 [Cucumis melo var. makuwa])

HSP 1 Score: 975.3 bits (2520), Expect = 2.2e-280
Identity = 490/560 (87.50%), Postives = 510/560 (91.07%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDIDLQRILEEFPQS  HVIMNSNQ++DEHSPIQLPTCAFCQRVL  D+DAT EPE IDI
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHR TPRGRRT   SS+S ENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA DF QHSSSHTTPNQSRRWRQ YSDSENDGLDSFDSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VS RTYGGDSDAS DGHSFLDTDIF+LP EGS  DTDTDIDPM AG+G
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIG 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           +WDSSDEE+D GEFTEA+TEQE+ S EARPQLQN + SSA  RGNSSNW E+LSSPEYEG
Sbjct: 241 NWDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           S  G IRRNRRFYSITNYAQSELLSY+GDSGDYLDRQGFE+LLEQIAETTSSRRGAPPAA
Sbjct: 301 STPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHP+CI PWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEEVKRSS NT   H LQP VVGLENPS LNGVGAN EFE+SQ ED
Sbjct: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
            RNIDSAV +SNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQR PGG+ TD RQ
Sbjct: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           LDA  A+SSNQRENRTRRWW
Sbjct: 541 LDARSAESSNQRENRTRRWW 556

[Close]

BLAST of Lsi01G016400 vs. NCBI nr
Match: XP_022950071.1 (uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata])

HSP 1 Score: 880.2 bits (2273), Expect = 9.6e-252
Identity = 450/560 (80.36%), Postives = 476/560 (85.00%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDID QRILEEFP STA +IM++NQQ++E  P+Q  TCAFC+ VL PDNDAT E EAI I
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HRTTPRGRRTRHGSS+S+ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           S H DRY DGSNAADF QHSSSHTTPNQSRRWRQ YSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS DGHSFLDTD FVLPDEGS VDTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           HWDSSDEE+DRGE TEADTE +         LQ+FH SSA GRGN  NW+E LSS EYEG
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYEG 300

Query: 301 SALGQIRRNRRFYSITNYAQSELLSYIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAA 360
           SA  QIRRN RFYSITNYA SE +SY+GDSGDYLDRQ FEELLEQIAETTSSRRGAPPAA
Sbjct: 301 SAREQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNS 420
           +SFVKNL RLVISKEHLKHDSISCAICKDF LVGVEVNQLPCLHLYHP CI PWL+ARNS
Sbjct: 361 ISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNS 420

Query: 421 CPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRVVGLENPSDLNGVGANPEFETSQGED 480
           CPLCR ELPTDDRDYEE K+SSTN  EI  LQ +V  LE+ SDLN VGANPEF+TSQGE+
Sbjct: 421 CPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNEVGANPEFDTSQGEE 480

Query: 481 QRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQ 540
             NID AV+NS NSGR GRR WLFVAAP+VGLVGIALM W GNP       GGR TDRRQ
Sbjct: 481 LSNIDPAVSNSYNSGR-GRRSWLFVAAPVVGLVGIALMFWVGNP------SGGRITDRRQ 540

Query: 541 LDAHIADSSNQRENRTRRWW 561
           LD   ADSSNQRENRTRRWW
Sbjct: 541 LDTRGADSSNQRENRTRRWW 545

[Close]

BLAST of Lsi01G016400 vs. NCBI nr
Match: XP_023543725.1 (E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 878.6 bits (2269), Expect = 2.8e-251
Identity = 454/585 (77.61%), Postives = 481/585 (82.22%), Query Frame = 0


Query: 1   MDIDLQRILEEFPQSTAHVIMNSNQQIDEHSPIQLPTCAFCQRVLVPDNDATGEPEAIDI 60
           MDID QRILEEFPQSTAH+IM++NQQ++E  P+Q  TCAFC+ VL P+NDAT E EAI I
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRTTPRGRRTRHGSSQSSENLFSQEFTHMINMVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HRTTPRGRRTRHGSS+S+ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SSHGDRYADGSNAADFTQHSSSHTTPNQSRRWRQAYSDSENDGLDSFDSMFGESESNFSF 180
           S HGDRY DGSNAADF QHSSSHTTPNQSRRWRQ YSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDAVSYRTYGGDSDASFDGHSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVG 240
           GPYRHSYGDSD VSYRTYGGDSDAS DGHSFLDTD FVLPDEG DVDTDTDIDPM AGV 
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEEDRGEFTEADTEQEVESFEARPQLQNFHASSAPGRGNSSNWDERLSSPEYEG 300
           HWDSSDEE+DRGE TEADTE +         LQ+FH SSA GRGN  NW+E LSS EYEG
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYEG 300

Query: 301 SAL-------------------------GQIRRNRRFYSITNYAQSELLSYIGDSGDYLD 360
           SA                          GQIRRN RFYSITNYAQSE +SY+GDSGDYLD
Sbjct: 301 SARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLD 360

Query: 361 RQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGV 420
           RQ FEELLEQIAETTSSRRGAPPAA+SFVKNL RLVISKEHLKHDSISCAICKDF LVGV
Sbjct: 361 RQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGV 420

Query: 421 EVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTTEIHGLQPRV 480
           EVNQLPCLHLYHP CI PWL+ARNSCPLCR ELPTDDRDYEE K+SSTN  EI  LQ +V
Sbjct: 421 EVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQV 480

Query: 481 VGLENPSDLNGVGANPEFETSQGEDQRNIDSAVNNSNNSGRGGRRRWLFVAAPIVGLVGI 540
             LE+ SDLNGVGANPEF+TSQGE+  NID AV+NS NSGR GRR WLFVAAP+VGLVGI
Sbjct: 481 TRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGR-GRRSWLFVAAPVVGLVGI 540

Query: 541 ALMLWFGNPLCDQRAPGGRFTDRRQLDAHIADSSNQRENRTRRWW 561
           ALM W GNP       GGR TDRRQLD   ADSSNQRE RTRRWW
Sbjct: 541 ALMFWVGNP------SGGRITDRRQLDTRGADSSNQREKRTRRWW 570

[Close]

BLAST of Lsi01G016400 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 9.7e-93
Identity = 230/535 (42.99%), Postives = 304/535 (56.82%), Query Frame = 0


Query: 38  CAFCQRVLVPDNDATGEPEAIDICGDCKFLLLEDIETPVRDSYHRTTPRGRR-------T 97
           C  C RV+     A+ + E   +C DCKFLLLED  TP      R T R RR       T
Sbjct: 24  CNLCGRVV---QSASDDLEIFSLCVDCKFLLLEDFGTPAPPLARRQTIRRRRMRRRRRTT 83

Query: 98  RHGSSQS--SENLFSQEFTHMINMVRQRQSSISSHGDRYADGSNAADFTQHSSSHTTPNQ 157
           RH SS+S    +L SQ+FTH+I++ RQ  S++ +  D   D          +SSHTTP+ 
Sbjct: 84  RHDSSESVGVGDLSSQQFTHLISIARQSLSTVQASEDLRDD--------LRTSSHTTPSG 143

Query: 158 SRRWRQAYSDSENDGLDSFDSMFGESESNFSFGPYRHSYGDSDAVSYRTYGGDSDASFDG 217
           S RWR  +S+SE+D  D+    FGE+ESN SF  YR  + D+D +S+  YGG+SDAS D 
Sbjct: 144 STRWR-LFSESESDDFDN----FGETESNASFSLYRFPHADNDTISFSAYGGESDASTDR 203

Query: 218 HSFLDTDIFVLPDEGSDVDTDTDIDPMHAGVGHWDSSDEEEDRGEFTEADTEQEVESFEA 277
           H+    DIFV PD+ SD+D DTDIDPM AG+  W+S  +EEDR E+ E      V    A
Sbjct: 204 HA----DIFVQPDDRSDIDFDTDIDPMRAGLNQWNS--DEEDR-EWEEGAGPSGV----A 263

Query: 278 RPQLQNFHASSAPGRGNSSNWDERLSSPEYEGSALGQIRRNRRFYS---ITNYAQSELLS 337
             + +N+ AS +    + + +D    SPE E S   +I   R   S    T     +   
Sbjct: 264 GTRYRNYLASPSESYSSMTRFD----SPELERSFRQRIIERRHSLSRNIFTGLEDLDFSP 323

Query: 338 YIGDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCA 397
           Y  + GDYLD +GF+ELLEQ+AE+ +SRRGAPPA+VS V+NLPR++I++EH+    + CA
Sbjct: 324 YAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHVM-KGLVCA 383

Query: 398 ICKDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNT 457
           ICK+   +  E  QLPCLHLYH  CI PWLSARNSCPLCR ELPTDD+DYEE KR+  + 
Sbjct: 384 ICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEEGKRNVLDV 443

Query: 458 TEIHGLQPRVVGLENPSDLNGVGANPEFETSQGEDQRNIDSAVNNSNNSGRGGRRRWLFV 517
           +E           ++ S  +G  +  E    +GE +  +           R  R RWLF+
Sbjct: 444 SE-----------DSSSSDDGTESGEEEYVERGESESGV----------SRVSRGRWLFL 489

Query: 518 -AAPIVGLVGIALMLWFGNPLCDQRAPGGRFTDRRQLDAHIADSSNQRENRTRRW 560
            AAP+V LVG+ L +W  +P             RR     IA S +QRENRTRRW
Sbjct: 504 AAAPVVSLVGVVLAMWLSSP------------HRR----GIAISHSQRENRTRRW 489

[Close]

BLAST of Lsi01G016400 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-27
Identity = 53/123 (43.09%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           G+ GDY    G E+L++Q+AE   +R G PPA+ S +  LP + ++K+ LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTTE 447
            D    G +V Q+PC H++H  C+ PWL   NSCP+CR ELPTDD DYE   + S  + +
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGD 279

Query: 448 IHG 451
             G
Sbjct: 280 GQG 282

[Close]

BLAST of Lsi01G016400 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 4.6e-26
Identity = 54/119 (45.38%), Postives = 73/119 (61.34%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           GD  DY+  Q ++ L EQ A+   S  G PP + SF+ NLP +++  E+     + CA+C
Sbjct: 255 GDHDDYV--QDYDMLFEQFADAEVSVIGLPPTSKSFLNNLPVVLLEGENDDDGGLVCAVC 314

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELPTDDRDYEEVKRSSTNTT 447
           KD + +G +  QLPC H YH  CI PWL  RN+CP+CR ELPTDD +YE+ K   T  T
Sbjct: 315 KDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDAEYEQRKTQRTTNT 371

[Close]

BLAST of Lsi01G016400 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 112.1 bits (279), Expect = 1.5e-24
Identity = 56/139 (40.29%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           G++GDY    G EEL EQ++  T +RRG PPA  S +  LP + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELP------------TDDRDY 447
           KD   +G E  Q+PC H+YH  CI PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 448 EEVKRSSTNTTEIHGLQPR 455
                SS++ +  +G + R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

[Close]

BLAST of Lsi01G016400 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 112.1 bits (279), Expect = 1.5e-24
Identity = 56/139 (40.29%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 328 GDSGDYLDRQGFEELLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 387
           G++GDY    G EEL EQ++  T +RRG PPA  S +  LP + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 388 KDFLLVGVEVNQLPCLHLYHPSCISPWLSARNSCPLCRCELP------------TDDRDY 447
           KD   +G E  Q+PC H+YH  CI PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

Query: 448 EEVKRSSTNTTEIHGLQPR 455
                SS++ +  +G + R
Sbjct: 255 RSSSSSSSSNSRENGNERR 272

The following BLAST results are available for this feature:

BLAST of Lsi01G016400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CH30	2.6e-26	46.55	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	3.4e-26	43.09	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
O22197	2.1e-23	40.29	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SPL2	4.6e-23	34.65	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]
Q94AK4	7.4e-21	42.15	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]

BLAST of Lsi01G016400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2Z4	7.4e-282	87.54	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 P...	[more]
A0A5D3C795	1.1e-280	87.50	E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3CIY7	1.1e-280	87.50	uncharacterized protein LOC103501491 OS=Cucumis melo OX=3656 GN=LOC103501491 PE=...	[more]
A0A6J1GDT0	4.7e-252	80.36	uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GEL6	8.2e-249	77.09	E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=366...	[more]

BLAST of Lsi01G016400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882118.1	4.7e-299	91.96	E3 ubiquitin-protein ligase Praja-2 [Benincasa hispida] >XP_038882119.1 E3 ubiqu...	[more]
XP_004142724.1	1.5e-281	87.54	E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus] >KGN54476.1 hypothetical p...	[more]
XP_008463301.1	2.2e-280	87.50	PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo] >TYK06209.1 E3 ub...	[more]
XP_022950071.1	9.6e-252	80.36	uncharacterized protein LOC111453262 isoform X2 [Cucurbita moschata]	[more]
XP_023543725.1	2.8e-251	77.61	E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi01G016400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01980.1	9.7e-93	42.99	RING/U-box superfamily protein	[more]
AT3G19950.1	2.4e-27	43.09	RING/U-box superfamily protein	[more]
AT5G08139.1	4.6e-26	45.38	RING/U-box superfamily protein	[more]
AT2G40830.2	1.5e-24	40.29	RING-H2 finger C1A	[more]
AT2G40830.1	1.5e-24	40.29	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 384..424 e-value: 6.7E-6 score: 35.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 383..425 e-value: 3.0E-9 score: 37.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 384..425 score: 11.854899
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 345..431 e-value: 4.8E-17 score: 63.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 266..294
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 469..495
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 115..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 133..164
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 460..495
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 235..300
None	No IPR available	PANTHER	PTHR15710:SF41	OS06G0101300 PROTEIN	coord: 318..479
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 318..479
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 365..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G016400.1	Lsi01G016400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity