Lsi01G016150 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G016150 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 2.4e-200
Identity = 340/582 (58.42%), Postives = 433/582 (74.40%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V+++   +RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAA 123
           V  DI   +   G +   +CSF EM + FN + G+    P G FN+ F +TG  Q+DAA+
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 132

Query: 124 TKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIG 183
           TKSL+  G++IPL   +  K  LVL   ++RAVP++WDP SLASFIEN+GTH++TS+TIG
Sbjct: 133 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 192

Query: 184 GKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQG 243
           G+DV+YI+QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K           
Sbjct: 193 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK----------- 252

Query: 244 VYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALLDG 303
                          D+ VIFRRRGGDDLEQ+H RWA TV ++PD+I+MTF PI +LL+G
Sbjct: 253 ---------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEG 312

Query: 304 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQFSI 363
           V G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQFS+
Sbjct: 313 VPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSL 372

Query: 364 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAIGA 423
           MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+H+ IGA
Sbjct: 373 MGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGA 432

Query: 424 PKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRN 483
           PKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+N
Sbjct: 433 PKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKN 492

Query: 484 VLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRNNADDSASGSKNIQGNKLAKL 543
           VL++KLL+S++P CTIRR++WDH P   S +L     + +++ +  SG    Q  KLAK+
Sbjct: 493 VLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLAKI 552

Query: 544 VDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           VD++EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 VDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Lsi01G016150 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 8.3e-116
Identity = 251/600 (41.83%), Postives = 357/600 (59.50%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIDMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQF 373
           GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C SLQF
Sbjct: 314 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 373

Query: 374 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 433
           S  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     D + +I
Sbjct: 374 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 433

Query: 434 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 493
                   E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G 
Sbjct: 434 ------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGF 493

Query: 494 RNVLYMKLLYSRLPECT-IRRTLWDHLPNDKSKK-----LVS-------------SSDNR 553
           +NVL+++L +SR+   T ++ + WD       K      L+S             +  N 
Sbjct: 494 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 553

Query: 554 NNADDSASGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 582
           N+A          Q  KL K VDT+EM++GPQ+ PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Lsi01G016150 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.9e-105
Identity = 234/599 (39.07%), Postives = 335/599 (55.93%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTIVQVDVEHSRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +V++D   +RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAATKSLSTD 130
            +       S I SF++M + FNQ   LS   PSG FN+ F F+     DA++ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYI 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI++
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDTHIAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + DT+  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPECTIRRTLWDHLPNDKSKKLV--------SSSDNRNNADDSASGSKNIQ 550
           L+++L +S       R++ WD+L  +  K  V        S+  + N A        +I 
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 554

Query: 551 G------------NKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLL 583
                         KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSLL
Sbjct: 555 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Lsi01G016150 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.6e-97
Identity = 226/622 (36.33%), Postives = 330/622 (53.05%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVQVDVEHSRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGF-TGFKQIDAAATKSLST 133
            +       S I  F++M +YFNQ+  ++   PSG+FN+ FGF +G    DAA  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQP 253
            ++Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++      P
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 TSAPYLTGKEDVA-------------VIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFY 313
            +      K+ VA             VI  +RGGD   ++H  W  TV   PD I+  F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDTHIAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D HI      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKK--------- 553
              +IVTGAQL V   GS++VL+++L Y+++ +  + +  W H P   S+K         
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSM 551

Query: 554 -LVSSSDNRNNAD--------DSA---SGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLV 584
            L S S + N           DS     G      NK+ K VD +++ +GPQ  PGHWLV
Sbjct: 552 PLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLV 611

BLAST of Lsi01G016150 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVRSSPDVI+MTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSKKLVSSSDNRNNADDSASGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KSKKLVSSSDN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lsi01G016150 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVRSSPDVI+MTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSKKLVSSSDNRNNADDSASGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KSKKLVSSSDN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lsi01G016150 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 546/584 (93.49%), Postives = 566/584 (96.92%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFNS FGF G KQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           A TK+LS DGFYIPLAKFQFTKTPLVLQENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVR SPDVI+MTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRNNADDSASGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTI+RTLWDHL NDKSKKLVSSSDN +NADDS+SGS+NIQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDN-SNADDSSSGSRNIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           KLVDT+EMSKGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Lsi01G016150 vs. ExPASy TrEMBL
Match: A0A0A0L2V7 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332620 PE=4 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 549/586 (93.69%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
            NV WDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG KQ DA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVRSSPDVI+MTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HIAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSKKLVSSSDNRNNADDSASGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KSKKLVSSSDN NNAD+S+S +KN QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lsi01G016150 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 542/585 (92.65%), Postives = 560/585 (95.73%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFN+ FGFTG KQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AA K+LSTDGFYIPLAKFQ T T L+L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPY +GKEDV VIFRRRGGDDLEQNHV+WA+TVRSSPDVI+MTFYPITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWDTHIAI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRN-NADDSASGSKNIQGNKL 540
           RNVLYMKLLYSRLP CTIRRTLWDHLPNDKSKKLVSSSDN N  ADDS+SGSKNIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           AKLVDTTEMSKGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lsi01G016150 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 552/584 (94.52%), Postives = 569/584 (97.43%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA+NAVQALGRGFDVNFD+RLLYCKGV+GSTIV++D EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNS FGFTGFKQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYIKQHQSSPLSTME+KRYVQDIANQRFSDTQGQ GSGSIK KEKGLDGGLFNN
Sbjct: 181 IGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGG+DLEQNHVRWAR VRSSPDVI+MTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYL+YKPPLEELRYFLEFQIPRVWAP  DTIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRNNADDSASGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTIRR LWDHLPNDKSKKLV+SSDN +  DDS+SG+K+IQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRMLWDHLPNDKSKKLVTSSDNSSKVDDSSSGTKSIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           K+VDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of Lsi01G016150 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVRSSPDVI+MTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSKKLVSSSDNRNNADDSASGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KSKKLVSSSDN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lsi01G016150 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 546/584 (93.49%), Postives = 566/584 (96.92%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFNS FGF G KQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           A TK+LS DGFYIPLAKFQFTKTPLVLQENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVR SPDVI+MTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRNNADDSASGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTI+RTLWDHL NDKSKKLVSSSDN +NADDS+SGS+NIQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDN-SNADDSSSGSRNIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           KLVDT+EMSKGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Lsi01G016150 vs. NCBI nr
Match: XP_011653720.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KGN54446.1 hypothetical protein Csa_011952 [Cucumis sativus])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 549/586 (93.69%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
            NV WDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG KQ DA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDV VIFRRRGGDDLEQNHVRWARTVRSSPDVI+MTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD HIAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSKKLVSSSDNRNNADDSASGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KSKKLVSSSDN NNAD+S+S +KN QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Lsi01G016150 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 542/585 (92.65%), Postives = 560/585 (95.73%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIV+VD EH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFN+ FGFTG KQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AA K+LSTDGFYIPLAKFQ T T L+L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALL 300
           QGVYPQPTSAPY +GKEDV VIFRRRGGDDLEQNHV+WA+TVRSSPDVI+MTFYPITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWDTHIAI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRN-NADDSASGSKNIQGNKL 540
           RNVLYMKLLYSRLP CTIRRTLWDHLPNDKSKKLVSSSDN N  ADDS+SGSKNIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           AKLVDTTEMSKGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Lsi01G016150 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 699.9 bits (1805), Expect = 1.7e-201
Identity = 340/582 (58.42%), Postives = 433/582 (74.40%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V+++   +RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAA 123
           V  DI   +   G +   +CSF EM + FN + G+    P G FN+ F +TG  Q+DAA+
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 132

Query: 124 TKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIG 183
           TKSL+  G++IPL   +  K  LVL   ++RAVP++WDP SLASFIEN+GTH++TS+TIG
Sbjct: 133 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 192

Query: 184 GKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQG 243
           G+DV+YI+QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K           
Sbjct: 193 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK----------- 252

Query: 244 VYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALLDG 303
                          D+ VIFRRRGGDDLEQ+H RWA TV ++PD+I+MTF PI +LL+G
Sbjct: 253 ---------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEG 312

Query: 304 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQFSI 363
           V G  HLTRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQFS+
Sbjct: 313 VPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSL 372

Query: 364 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAIGA 423
           MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+H+ IGA
Sbjct: 373 MGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGA 432

Query: 424 PKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRN 483
           PKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+N
Sbjct: 433 PKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKN 492

Query: 484 VLYMKLLYSRLPECTIRRTLWDHLPNDKSKKLVSSSDNRNNADDSASGSKNIQGNKLAKL 543
           VL++KLL+S++P CTIRR++WDH P   S +L     + +++ +  SG    Q  KLAK+
Sbjct: 493 VLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLAKI 552

Query: 544 VDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           VD++EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 VDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Lsi01G016150 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 419.1 bits (1076), Expect = 5.9e-117
Identity = 251/600 (41.83%), Postives = 357/600 (59.50%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIDMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQF 373
           GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C SLQF
Sbjct: 314 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 373

Query: 374 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHIAI 433
           S  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     D + +I
Sbjct: 374 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 433

Query: 434 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 493
                   E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G 
Sbjct: 434 ------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGF 493

Query: 494 RNVLYMKLLYSRLPECT-IRRTLWDHLPNDKSKK-----LVS-------------SSDNR 553
           +NVL+++L +SR+   T ++ + WD       K      L+S             +  N 
Sbjct: 494 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 553

Query: 554 NNADDSASGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 582
           N+A          Q  KL K VDT+EM++GPQ+ PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Lsi01G016150 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 383.6 bits (984), Expect = 2.7e-106
Identity = 234/599 (39.07%), Postives = 335/599 (55.93%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTIVQVDVEHSRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +V++D   +RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAATKSLSTD 130
            +       S I SF++M + FNQ   LS   PSG FN+ F F+     DA++ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYI 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI++
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVAVIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFYPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP-GHQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDTHIAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + DT+  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPECTIRRTLWDHLPNDKSKKLV--------SSSDNRNNADDSASGSKNIQ 550
           L+++L +S       R++ WD+L  +  K  V        S+  + N A        +I 
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKIDIN 554

Query: 551 G------------NKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLL 583
                         KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSLL
Sbjct: 555 SAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Lsi01G016150 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 356.3 bits (913), Expect = 4.7e-98
Identity = 226/622 (36.33%), Postives = 330/622 (53.05%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVQVDVEHSRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGF-TGFKQIDAAATKSLST 133
            +       S I  F++M +YFNQ+  ++   PSG+FN+ FGF +G    DAA  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQP 253
            ++Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++      P
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 TSAPYLTGKEDVA-------------VIFRRRGGDDLEQNHVRWARTVRSSPDVIDMTFY 313
            +      K+ VA             VI  +RGGD   ++H  W  TV   PD I+  F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIP--GHQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV + +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDTHIAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D HI      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPECTIRRTLWDHLPNDKSKK--------- 553
              +IVTGAQL V   GS++VL+++L Y+++ +  + +  W H P   S+K         
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSM 551

Query: 554 -LVSSSDNRNNAD--------DSA---SGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLV 584
            L S S + N           DS     G      NK+ K VD +++ +GPQ  PGHWLV
Sbjct: 552 PLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLV 611

BLAST of Lsi01G016150 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-52
Identity = 127/332 (38.25%), Postives = 191/332 (57.53%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTIVQVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSGFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVAVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIDMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKP--PLEELRYFLE 332
           GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 GVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335