Lsi01G015810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G015810
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	chr01: 14005403 .. 14006032 (+)
RNA-Seq Expression	Lsi01G015810
Synteny	Lsi01G015810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTAATCATGTCTTTAGCGAGTTCAAATCCTATTCAGTATCGTCTGTACGGCACCGACATCAGCAATAGAGTATTGGAAGTTAATTTACGTTACCTCGAACGTACAATCTCTATTTCTCCGGTCGCCGGACATCCACCGCGAATCGTCCATGAAAGTCCACCGACTCCATTCCACAATGCACTCTTCTATCTCTCTTTACGCCAACTTCAAGATCCGTTGTTCTACATTCGGCAATTCCTCTCCTCACTCAGCATTCTCCCCACCGCTTGCGAAGTTATTTCTCGGCAAATCGCTTCCTACTTCGTCAACAGAGAGAGTAATTTCCATATTATCGCTGAAATCGATTTCGTCCTAGTGATCTGGATGGAAGCTGAGCAATCAATCGCCGATGAAGCTCCGCCGCCTAGGAGAGGAGTGGCGGTGGAGAGGGTGAAATCGGAAGAGAAAGGAGAGGAATTGGGAGATTGCAGCGTTTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATAAGAATCCCGTGCGGGCATGTGTATCATAAATCATGCATCTTCAACCGGCTTAGGAACTATAATTCTTGCCCTTTGTGTAGAAAACCATTGCCACTAGAGGACCCAAAGTGA

mRNA sequence

ATGGAATTAATCATGTCTTTAGCGAGTTCAAATCCTATTCAGTATCGTCTGTACGGCACCGACATCAGCAATAGAGTATTGGAAGTTAATTTACGTTACCTCGAACGTACAATCTCTATTTCTCCGGTCGCCGGACATCCACCGCGAATCGTCCATGAAAGTCCACCGACTCCATTCCACAATGCACTCTTCTATCTCTCTTTACGCCAACTTCAAGATCCGTTGTTCTACATTCGGCAATTCCTCTCCTCACTCAGCATTCTCCCCACCGCTTGCGAAGTTATTTCTCGGCAAATCGCTTCCTACTTCGTCAACAGAGAGAGTAATTTCCATATTATCGCTGAAATCGATTTCGTCCTAGTGATCTGGATGGAAGCTGAGCAATCAATCGCCGATGAAGCTCCGCCGCCTAGGAGAGGAGTGGCGGTGGAGAGGGTGAAATCGGAAGAGAAAGGAGAGGAATTGGGAGATTGCAGCGTTTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATAAGAATCCCGTGCGGGCATGTGTATCATAAATCATGCATCTTCAACCGGCTTAGGAACTATAATTCTTGCCCTTTGTGTAGAAAACCATTGCCACTAGAGGACCCAAAGTGA

Coding sequence (CDS)

ATGGAATTAATCATGTCTTTAGCGAGTTCAAATCCTATTCAGTATCGTCTGTACGGCACCGACATCAGCAATAGAGTATTGGAAGTTAATTTACGTTACCTCGAACGTACAATCTCTATTTCTCCGGTCGCCGGACATCCACCGCGAATCGTCCATGAAAGTCCACCGACTCCATTCCACAATGCACTCTTCTATCTCTCTTTACGCCAACTTCAAGATCCGTTGTTCTACATTCGGCAATTCCTCTCCTCACTCAGCATTCTCCCCACCGCTTGCGAAGTTATTTCTCGGCAAATCGCTTCCTACTTCGTCAACAGAGAGAGTAATTTCCATATTATCGCTGAAATCGATTTCGTCCTAGTGATCTGGATGGAAGCTGAGCAATCAATCGCCGATGAAGCTCCGCCGCCTAGGAGAGGAGTGGCGGTGGAGAGGGTGAAATCGGAAGAGAAAGGAGAGGAATTGGGAGATTGCAGCGTTTGTTTGGATGAATTGAGTTGTGAAAAGAGGGAGGTGATAAGAATCCCGTGCGGGCATGTGTATCATAAATCATGCATCTTCAACCGGCTTAGGAACTATAATTCTTGCCCTTTGTGTAGAAAACCATTGCCACTAGAGGACCCAAAGTGA

Protein sequence

MELIMSLASSNPIQYRLYGTDISNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNALFYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFVNRESNFHIIAEIDFVLVIWMEAEQSIADEAPPPRRGVAVERVKSEEKGEELGDCSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLEDPK

Homology

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BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.4e-09
Identity = 44/148 (29.73%), Postives = 68/148 (45.95%), Query Frame = 0


Query: 73  DPLFYIRQFLSSLSILPTACEVISRQIASYFVNRESNFHIIAEI-DFVLVIWME-AEQSI 132
           DP  +I+  L+ L       E + +   S     +  F + A I D+ +   +E   Q +
Sbjct: 132 DPFTFIQNHLNDLRSSGAQIEFVIQNNPS-----DQGFRLPANIGDYFIGPGLEQLIQQL 191

Query: 133 ADEAP-----PPRRGVAVE-----RVKSEEKGEELGDCSVCLDELSCEKREVIRIPCGHV 192
           A+  P     PP    A+E      +       E   C+VC+D+   E  E  ++PC H+
Sbjct: 192 AENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFE-EGTEAKQMPCKHL 251

Query: 193 YHKSCIFNRLRNYNSCPLCRKPLPLEDP 209
           YHK C+   L  +NSCP+CR  LP +DP
Sbjct: 252 YHKDCLLPWLELHNSCPVCRHELPTDDP 273

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BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 31/91 (34.07%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 128 QSIADEAP-----PPRRGVAVE-----RVKSEEKGEELGDCSVCLDELSCEKREVIRIPC 187
           Q +A+  P     PP    A++     +V  +    E+  C+VC+DE   +  +V ++PC
Sbjct: 176 QQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPC 235

Query: 188 GHVYHKSCIFNRLRNYNSCPLCRKPLPLEDP 209
            HV+H+ C+   L  +NSCP+CR  LP +DP
Sbjct: 236 KHVFHQDCLLPWLELHNSCPVCRFELPTDDP 265

[Close]

BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot
Match: Q9CY62 (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-07
Identity = 31/75 (41.33%), Postives = 38/75 (50.67%), Query Frame = 0


Query: 135 PPPRRGVAVERVKSEEKGEELGD--CSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLRN 194
           PPP     VE +          D  C VCL E   E+  VI +PC H++H +CI   L  
Sbjct: 63  PPPAAKAVVESLPRTVISSAKADLKCPVCLLEFEAEE-TVIEMPCHHLFHSNCILPWLSK 122

Query: 195 YNSCPLCRKPLPLED 208
            NSCPLCR  LP +D
Sbjct: 123 TNSCPLCRHELPTDD 136

[Close]

BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-07
Identity = 33/76 (43.42%), Postives = 39/76 (51.32%), Query Frame = 0


Query: 135 PPPRRGVAVE---RVKSEEKGEELGDCSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLR 194
           PPP     VE   R        EL  C VCL E   E+  VI +PC H++H +CI   L 
Sbjct: 63  PPPAAKAVVESLPRTVIRSSKAEL-KCPVCLLEFE-EEETVIEMPCHHLFHSNCILPWLS 122

Query: 195 NYNSCPLCRKPLPLED 208
             NSCPLCR  LP +D
Sbjct: 123 KTNSCPLCRHELPTDD 136

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BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-07
Identity = 36/100 (36.00%), Postives = 52/100 (52.00%), Query Frame = 0


Query: 115 EIDFVLVIWMEAEQSIADEAPPPRRGVAVERVK----SEEKGEELGDCSVCLDELSCEKR 174
           +ID   + + E +Q +    PPP     VE +     + E+ +    C VCL  L  E+ 
Sbjct: 36  DIDLGALDFTEWDQRL----PPPAAKKVVESLPKVTVTPEQADAALKCPVCL--LEFEEG 95

Query: 175 EVIR-IPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLEDPK 210
           E +R +PC H++H SCI   L   NSCPLCR  LP + P+
Sbjct: 96  ETVRQLPCEHLFHSSCILPWLGKTNSCPLCRHELPTDSPE 129

[Close]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 135/223 (60.54%), Postives = 168/223 (75.34%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLYG+    N +LEV + + E+ IS+SPV G P +I+H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
            YL+LRQLQ PLFYI QFLSSL+I P A + ++RQIAS+ +     N + NFHIIAE+DF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 125 VLVIWMEAEQS----------IADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDE 184
           + VIW+E E+           + DEAPP  +RGV     ER+KSEEK E+LGDCS+CLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 185 LSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           LSCEKREVIRIPCGHVYH+SCIF  L+N+NSCPLCRKPLPL++
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKE 223

[Close]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 135/223 (60.54%), Postives = 168/223 (75.34%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLYG+    N +LEV + + E+ IS+SPV G P +I+H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
            YL+LRQLQ PLFYI QFLSSL+I P A + ++RQIAS+ +     N + NFHIIAE+DF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 125 VLVIWMEAEQS----------IADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDE 184
           + VIW+E E+           + DEAPP  +RGV     ER+KSEEK E+LGDCS+CLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 185 LSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           LSCEKREVIRIPCGHVYH+SCIF  L+N+NSCPLCRKPLPL++
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKE 223

[Close]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.7e-64
Identity = 129/222 (58.11%), Postives = 166/222 (74.77%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLY +    N VL+VN+ ++++ IS+SP+ G P  ++H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
           FYLSLRQLQ PLFYI Q LSSL+I P A + ++R+IAS+ +     N E+NFHI+AE+DF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 125 VLVIWMEA---------EQSIADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDEL 184
           + +IW+ +         E  + DEAPP  +RGV V   ER++SEEK EELGDCS+CLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 185 SCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           SCEKREV+RIPCGHVYH+SCIF  L N+NSCPLCRKPL  +D
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD 222

[Close]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL
Match: A0A0A0K2W2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.7e-64
Identity = 129/222 (58.11%), Postives = 166/222 (74.77%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLY +    N VL+VN+ ++++ IS+SP+ G P  ++H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
           FYLSLRQLQ PLFYI Q LSSL+I P A + ++R+IAS+ +     N E+NFHI+AE+DF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 125 VLVIWMEA---------EQSIADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDEL 184
           + +IW+ +         E  + DEAPP  +RGV V   ER++SEEK EELGDCS+CLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 185 SCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           SCEKREV+RIPCGHVYH+SCIF  L N+NSCPLCRKPL  +D
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD 222

[Close]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL
Match: A0A5A7TLM1 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001430 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 3.1e-52
Identity = 125/226 (55.31%), Postives = 148/226 (65.49%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDISNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNALF 64
           MS A S+PIQY L GT   N VLE+ L Y+ER ISIS V+    RI++E+ P PF+NA F
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVS---QRILYETQPIPFNNAHF 60

Query: 65  YLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFVN-------RESNFHIIAEID 124
           YLS+  LQDPLFYIRQFL SL+I     E I+RQIASY  +       R SNF++IA +D
Sbjct: 61  YLSVHHLQDPLFYIRQFLFSLNI---RRENIARQIASYVAHMCNGNTGRNSNFYVIARVD 120

Query: 125 FVLVI-------W-------MEAEQSIADEAPPPRRGVAV-ERVKSEEKGEELGDCSVCL 184
            V  I       W       +EAE  + DE P  RRGV V ER++  +  EE GDCSVCL
Sbjct: 121 LVRAIRIEEESMWRDGGGVVVEAETVVFDEVPRARRGVVVGERLRKLKSEEEEGDCSVCL 180

Query: 185 DELSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLEDP 209
           DEL C KREVIRIPCGHVYH+SCIF  L N NSCPLCR   PL++P
Sbjct: 181 DELDCGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCRTSFPLDNP 220

[Close]

BLAST of Lsi01G015810 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 284.3 bits (726), Expect = 8.7e-73
Identity = 149/224 (66.52%), Postives = 168/224 (75.00%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGT--DISNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNA 64
           MS A+S PIQYRLYGT     N VLEV   Y ER IS+SPV G PPRI+HE+P  PFHNA
Sbjct: 1   MSSAASTPIQYRLYGTGSGTGNGVLEVQTSYFERMISVSPVTGRPPRILHETPRIPFHNA 60

Query: 65  LFYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYF-----VNRESNFHIIAEID 124
           LFYLSL QLQ+PLFYI QFLSSL+I P ACE+++RQIASY       NRESNF IIAE+D
Sbjct: 61  LFYLSLHQLQNPLFYISQFLSSLNICPAACEIMARQIASYIDRMINENRESNFRIIAEVD 120

Query: 125 FVLVIWME----------AEQSIA-DEAPPPRRG---VAVERVKSEEKGEELGDCSVCLD 184
           F  VIWME          AE+++  DEAPPPRRG   VAVERV+  +  EELGDC +CL+
Sbjct: 121 FSRVIWMEEHSIYGGDAMAEETVVFDEAPPPRRGVEKVAVERVRELKSQEELGDCCICLE 180

Query: 185 ELSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           ELSCEKREVIRIPCGHVYH+SC+F  LR  NSCPLCRKPLPL D
Sbjct: 181 ELSCEKREVIRIPCGHVYHESCVFKWLRKNNSCPLCRKPLPLND 224

[Close]

BLAST of Lsi01G015810 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 263.5 bits (672), Expect = 1.6e-66
Identity = 135/223 (60.54%), Postives = 168/223 (75.34%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLYG+    N +LEV + + E+ IS+SPV G P +I+H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
            YL+LRQLQ PLFYI QFLSSL+I P A + ++RQIAS+ +     N + NFHIIAE+DF
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHIIAEVDF 120

Query: 125 VLVIWMEAEQS----------IADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDE 184
           + VIW+E E+           + DEAPP  +RGV     ER+KSEEK E+LGDCS+CLDE
Sbjct: 121 IRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICLDE 180

Query: 185 LSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           LSCEKREVIRIPCGHVYH+SCIF  L+N+NSCPLCRKPLPL++
Sbjct: 181 LSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLKE 223

[Close]

BLAST of Lsi01G015810 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 254.2 bits (648), Expect = 9.7e-64
Identity = 129/222 (58.11%), Postives = 166/222 (74.77%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLY +    N VL+VN+ ++++ IS+SP+ G P  ++H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
           FYLSLRQLQ PLFYI Q LSSL+I P A + ++R+IAS+ +     N E+NFHI+AE+DF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 125 VLVIWMEA---------EQSIADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDEL 184
           + +IW+ +         E  + DEAPP  +RGV V   ER++SEEK EELGDCS+CLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 185 SCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           SCEKREV+RIPCGHVYH+SCIF  L N+NSCPLCRKPL  +D
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD 222

[Close]

BLAST of Lsi01G015810 vs. NCBI nr
Match: XP_011659694.1 (E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical protein Csa_020163 [Cucumis sativus])

HSP 1 Score: 254.2 bits (648), Expect = 9.7e-64
Identity = 129/222 (58.11%), Postives = 166/222 (74.77%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDI-SNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNAL 64
           MS A SNPIQYRLY +    N VL+VN+ ++++ IS+SP+ G P  ++H++P  P+HNAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 65  FYLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFV-----NRESNFHIIAEIDF 124
           FYLSLRQLQ PLFYI Q LSSL+I P A + ++R+IAS+ +     N E+NFHI+AE+DF
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHILAEVDF 120

Query: 125 VLVIWMEA---------EQSIADEAPPP-RRGVAV---ERVKSEEKGEELGDCSVCLDEL 184
           + +IW+ +         E  + DEAPP  +RGV V   ER++SEEK EELGDCS+CLDEL
Sbjct: 121 IRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELGDCSICLDEL 180

Query: 185 SCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLED 208
           SCEKREV+RIPCGHVYH+SCIF  L N+NSCPLCRKPL  +D
Sbjct: 181 SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLHHDD 222

[Close]

BLAST of Lsi01G015810 vs. NCBI nr
Match: XP_016901482.1 (PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29540.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 214.9 bits (546), Expect = 6.5e-52
Identity = 125/226 (55.31%), Postives = 148/226 (65.49%), Query Frame = 0


Query: 5   MSLASSNPIQYRLYGTDISNRVLEVNLRYLERTISISPVAGHPPRIVHESPPTPFHNALF 64
           MS A S+PIQY L GT   N VLE+ L Y+ER ISIS V+    RI++E+ P PF+NA F
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVS---QRILYETQPIPFNNAHF 60

Query: 65  YLSLRQLQDPLFYIRQFLSSLSILPTACEVISRQIASYFVN-------RESNFHIIAEID 124
           YLS+  LQDPLFYIRQFL SL+I     E I+RQIASY  +       R SNF++IA +D
Sbjct: 61  YLSVHHLQDPLFYIRQFLFSLNI---RRENIARQIASYVAHMCNGNTGRNSNFYVIARVD 120

Query: 125 FVLVI-------W-------MEAEQSIADEAPPPRRGVAV-ERVKSEEKGEELGDCSVCL 184
            V  I       W       +EAE  + DE P  RRGV V ER++  +  EE GDCSVCL
Sbjct: 121 LVRAIRIEEESMWRDGGGVVVEAETVVFDEVPRARRGVVVGERLRKLKSEEEEGDCSVCL 180

Query: 185 DELSCEKREVIRIPCGHVYHKSCIFNRLRNYNSCPLCRKPLPLEDP 209
           DEL C KREVIRIPCGHVYH+SCIF  L N NSCPLCR   PL++P
Sbjct: 181 DELDCGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCRTSFPLDNP 220

[Close]

BLAST of Lsi01G015810 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.6e-10
Identity = 31/91 (34.07%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 128 QSIADEAP-----PPRRGVAVE-----RVKSEEKGEELGDCSVCLDELSCEKREVIRIPC 187
           Q +A+  P     PP    A++     +V  +    E+  C+VC+DE   +  +V ++PC
Sbjct: 176 QQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPC 235

Query: 188 GHVYHKSCIFNRLRNYNSCPLCRKPLPLEDP 209
            HV+H+ C+   L  +NSCP+CR  LP +DP
Sbjct: 236 KHVFHQDCLLPWLELHNSCPVCRFELPTDDP 265

[Close]

BLAST of Lsi01G015810 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.6e-10
Identity = 30/70 (42.86%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 139 RGVAVERVKSEEKGEELGDCSVCLDELSCEKREVIRIP-CGHVYHKSCIFNRLRNYNSCP 198
           R V  +RV+S +  EE   CS+C+++ S    ++IR+P C H++H+ C+F  L   NSCP
Sbjct: 140 RLVEEQRVESADLEEEDETCSICIEKFSESHEDIIRVPDCLHLFHQGCLFEWLGLQNSCP 199

Query: 199 LCRKPLPLED 208
           LCRK +P E+
Sbjct: 200 LCRK-VPYEE 208

[Close]

BLAST of Lsi01G015810 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 135 PPPRRGV-AVERVKSEEKGEELGD--CSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLR 194
           P P+  + A+  +K  +K  +  D  C VC DE    K E  ++PC H+YH  CI   L 
Sbjct: 160 PAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWLV 219

Query: 195 NYNSCPLCRKPLP 205
            +NSCP+CRK LP
Sbjct: 220 QHNSCPVCRKELP 231

[Close]

BLAST of Lsi01G015810 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 135 PPPRRGV-AVERVKSEEKGEELGD--CSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLR 194
           P P+  + A+  +K  +K  +  D  C VC DE    K E  ++PC H+YH  CI   L 
Sbjct: 160 PAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWLV 219

Query: 195 NYNSCPLCRKPLP 205
            +NSCP+CRK LP
Sbjct: 220 QHNSCPVCRKELP 231

[Close]

BLAST of Lsi01G015810 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.6e-08
Identity = 30/73 (41.10%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 135 PPPRRGV-AVERVKSEEKGEELGD--CSVCLDELSCEKREVIRIPCGHVYHKSCIFNRLR 194
           P P+  + A+  +K  +K  +  D  C VC DE    K E  ++PC H+YH  CI   L 
Sbjct: 160 PAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFEL-KSEAKQMPCHHIYHSDCIVPWLV 219

Query: 195 NYNSCPLCRKPLP 205
            +NSCP+CRK LP
Sbjct: 220 QHNSCPVCRKELP 231

The following BLAST results are available for this feature:

BLAST of Lsi01G015810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CH30	2.4e-09	29.73	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	1.2e-08	34.07	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9CY62	1.0e-07	41.33	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1	[more]
Q6AXU4	1.7e-07	43.42	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
Q5M974	1.7e-07	36.00	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ...	[more]

BLAST of Lsi01G015810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E1S5	7.7e-67	60.54	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	7.7e-67	60.54	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]
A0A0A0K696	4.7e-64	58.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P...	[more]
A0A0A0K2W2	4.7e-64	58.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 P...	[more]
A0A5A7TLM1	3.1e-52	55.31	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Lsi01G015810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883574.1	8.7e-73	66.52	E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida]	[more]
XP_016899530.1	1.6e-66	60.54	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ...	[more]
XP_004144954.1	9.7e-64	58.11	uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ...	[more]
XP_011659694.1	9.7e-64	58.11	E3 ubiquitin-protein ligase RDUF1 [Cucumis sativus] >KGN43356.1 hypothetical pro...	[more]
XP_016901482.1	6.5e-52	55.31	PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ...	[more]

BLAST of Lsi01G015810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	8.6e-10	34.07	RING/U-box superfamily protein	[more]
AT5G37200.1	8.6e-10	42.86	RING/U-box superfamily protein	[more]
AT3G56580.2	1.6e-08	41.10	RING/U-box superfamily protein	[more]
AT3G56580.1	1.6e-08	41.10	RING/U-box superfamily protein	[more]
AT3G56580.3	1.6e-08	41.10	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 158..199 e-value: 8.1E-8 score: 42.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 157..200 e-value: 3.2E-10 score: 40.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 158..200 score: 12.563043
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 125..205 e-value: 1.0E-15 score: 59.2
None	No IPR available	PANTHER	PTHR22765:SF329	SUBFAMILY NOT NAMED	coord: 11..201
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 11..201
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 157..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G015810.1	Lsi01G015810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0061630	ubiquitin protein ligase activity