Lsi01G013460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G013460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionplasma membrane intrinsic protein 2
Locationchr01: 11656610 .. 11658914 (-)
RNA-Seq ExpressionLsi01G013460
SyntenyLsi01G013460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGATAAAGCATTTAATATGACTCTTAAATTCATATTAATGACATTAGCTTAAAACCTTAATTTAAATTATGAGAATTAAATCAACAATGTTTATGCTTCTCAAGGCATTCTCTTCATATAACAAGAGTACAAAAATCAACTAATATATCCTCAATACAATTAGATATGAAGAATTGAACTGAATAAAAACCAGAGGGAAAAGACAGATTGAAGAAGATGAGTTTTGAGCACTGAGCTGGTCATACAATTCTCATTAAATAACCAAAATGGAATACAATGAATGGAGCAACTTGACAAAGTGAGTACAGAGGAAATAAATAACAAACAATGACAGTTTTCTACAACTTGAAGCTTACGGTTTATTTGAAATGACCGTGTTTAGTTTTATGTGGAAATGTTATTAGATTTTTGTATGAACAAATTGTAATACTGAGATGCACTTATCTTTGTACATCTCACTCTCATCACACACATGAAAAATTATGATTGGAGAATTTGGTATTACTTGTAATGCACTCGAATATTTTTTTTCTAAAATTAATATATAAATTTTAGGTAATTATGATACATTATTGGAAAATTTTCACATATAAAAAGAATGTCAAACTATTTATATAACATAGAAAAAAAAAAAAAAAAAAAAAACACTTATAGATATTGATAAACTTTTTTCAGCTATCAGTGATAAGACTTTTATCATTTCTATCACTGATAGATCACAACTATCTAAAATTTTTACTACTGTGTGTAAATATTTTCTTTTATTTTACTATTTTTAAAAATGTCCTTACATTATTTGGCTAAAAAGAATAATGGATTAATATTATTATCTAACATTTTCAACCAATTACTATCAGTGAAATTTTCTATAATTTGCTTATATAGGAAAAGAAACTTCAATAAATGAAGTTAACTCGAAGATAGTTTAATTGACCTACAACATACATCTTCGACTAAGAGATCAGACTTTATTTGAATCTTCCGATATAAATTATTGTTAAAAAAAAATTCATAAAGGAAGAAAACCCTACATTTGGGAGAGTATAGATACCCTCTCCAAAGGTATATGAAACGCTAAGGAATAGATTATTCCTATTTTCCAAACTTACATAACATAAACCAAATAACAAAATATATTTAATTTACCAAATTCAACTTCATAAATTCATAACTCTCCTCTGGTTTTCAACACAGCAGGAGCAATGTCTAACAATGTTGATGGAAGAAGAAAGGTCAAGGACTACGAAGATCCACCTCCCGCCCTGTTCATCGACGCCGGCGAGCTCATTCAGTGGTCCTTTTACAGAGCCATTATCGCCGAGTTCGTCGCCACCCTTCTCTTCTTGTACATTCTTGTTCTCACTGTCATTGGCAATTCCAGCCTCTCCGCCGCCAATACCTGCGGTGGTATCGGCGCTTTGGGCATTTCCTGGGCTGTCGGCGGCATGATCTTCGTTCTCGTTTACTGCACCGCTGGAATTTCTGGTGAGTTTAGTTTTCTTCTATTGCTCTGTTTCTCACTCTGTTAGATCTGTCAAATTACGCTGTGTTGTTCATCTCTGTAGGTGGGCATATTAATCCAGCGGTGACGTTTGGGATGTTCTTAGCTCGAAAAATCTCTCTAATCAGAGCTTTTTTTTACATTGTGGCTCAATGTTTGGGCGCCGTTTGTGGGTGTGCCTTGGCTAAATCATTACAGAAGACTTATTACGTTCAGTACCAAGGTGCAGCTAATATGGTCGCCGATGGGTACAGCATCGGCACCGGCTTAGCCGCAGAGATCATTGGAACTTTTGTTCTTGTTTACACAGTCTTCTCCGCCACTGATCCCAAGAGAAACGCTAGAGATTCTCACATCCCTGTAAGTTAGTTTAATCTACACAAATTGGGTTTATGCTCTGTTTTTCACCATTCAATTAACTAATCAAGTTTGAACTTAAAAACCAAAGGTTTTGGCGCCACTCCCAATTGGATTCGCAGTGATTATAGTTCATTTAGCCACCATTCCAATCACCGGCACCGGCATCAACCCGGCTCGAAGCTTCGGAGCTGCAATGATCTATAACAAAGCCAAGGCCTGGGATCATCATGTAAATTTTCTCCCCAAATTCTTCTCTTTAGTCTTTTGTATCTTTGTTTAAGAAAATTTGAAGCTTATTTCATTTTCTGTTTTGAACAGTGGATCTTTTGGATTGGGCCATTCATTGGAGCTGCCATTGCTGCAGTTTATCATGTAGTTATAATAAGGGCAGGGACAATTAAAGCTCTGGTTTCCTTCAGAAGTTCCTCTGCTCTATAA

mRNA sequence

ATGGGAGCAATGTCTAACAATGTTGATGGAAGAAGAAAGGTCAAGGACTACGAAGATCCACCTCCCGCCCTGTTCATCGACGCCGGCGAGCTCATTCAGTGGTCCTTTTACAGAGCCATTATCGCCGAGTTCGTCGCCACCCTTCTCTTCTTGTACATTCTTGTTCTCACTGTCATTGGCAATTCCAGCCTCTCCGCCGCCAATACCTGCGGTGGTATCGGCGCTTTGGGCATTTCCTGGGCTGTCGGCGGCATGATCTTCGTTCTCGTTTACTGCACCGCTGGAATTTCTGATCTGTCAAATTACGCTGTGTTGTTCATCTCTGTAGGTGGGCATATTAATCCAGCGGTGACGTTTGGGATGTTCTTAGCTCGAAAAATCTCTCTAATCAGAGCTTTTTTTTACATTGTGGCTCAATGTTTGGGCGCCGTTTGTGGGTGTGCCTTGGCTAAATCATTACAGAAGACTTATTACGTTCAGTACCAAGGTGCAGCTAATATGGTCGCCGATGGGTACAGCATCGGCACCGGCTTAGCCGCAGAGATCATTGGAACTTTTGTTCTTGTTTACACAGTCTTCTCCGCCACTGATCCCAAGAGAAACGCTAGAGATTCTCACATCCCTGTTTTGGCGCCACTCCCAATTGGATTCGCAGTGATTATAGTTCATTTAGCCACCATTCCAATCACCGGCACCGGCATCAACCCGGCTCGAAGCTTCGGAGCTGCAATGATCTATAACAAAGCCAAGGCCTGGGATCATCATTGGATCTTTTGGATTGGGCCATTCATTGGAGCTGCCATTGCTGCAGTTTATCATGTAGTTATAATAAGGGCAGGGACAATTAAAGCTCTGGTTTCCTTCAGAAGTTCCTCTGCTCTATAA

Coding sequence (CDS)

ATGGGAGCAATGTCTAACAATGTTGATGGAAGAAGAAAGGTCAAGGACTACGAAGATCCACCTCCCGCCCTGTTCATCGACGCCGGCGAGCTCATTCAGTGGTCCTTTTACAGAGCCATTATCGCCGAGTTCGTCGCCACCCTTCTCTTCTTGTACATTCTTGTTCTCACTGTCATTGGCAATTCCAGCCTCTCCGCCGCCAATACCTGCGGTGGTATCGGCGCTTTGGGCATTTCCTGGGCTGTCGGCGGCATGATCTTCGTTCTCGTTTACTGCACCGCTGGAATTTCTGATCTGTCAAATTACGCTGTGTTGTTCATCTCTGTAGGTGGGCATATTAATCCAGCGGTGACGTTTGGGATGTTCTTAGCTCGAAAAATCTCTCTAATCAGAGCTTTTTTTTACATTGTGGCTCAATGTTTGGGCGCCGTTTGTGGGTGTGCCTTGGCTAAATCATTACAGAAGACTTATTACGTTCAGTACCAAGGTGCAGCTAATATGGTCGCCGATGGGTACAGCATCGGCACCGGCTTAGCCGCAGAGATCATTGGAACTTTTGTTCTTGTTTACACAGTCTTCTCCGCCACTGATCCCAAGAGAAACGCTAGAGATTCTCACATCCCTGTTTTGGCGCCACTCCCAATTGGATTCGCAGTGATTATAGTTCATTTAGCCACCATTCCAATCACCGGCACCGGCATCAACCCGGCTCGAAGCTTCGGAGCTGCAATGATCTATAACAAAGCCAAGGCCTGGGATCATCATTGGATCTTTTGGATTGGGCCATTCATTGGAGCTGCCATTGCTGCAGTTTATCATGTAGTTATAATAAGGGCAGGGACAATTAAAGCTCTGGTTTCCTTCAGAAGTTCCTCTGCTCTATAA

Protein sequence

MGAMSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL
Homology
BLAST of Lsi01G013460 vs. ExPASy Swiss-Prot
Match: P43287 (Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 397.1 bits (1019), Expect = 1.7e-109
Identity = 203/295 (68.81%), Postives = 235/295 (79.66%), Query Frame = 0

Query: 4   MSNNVDGRR--KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG- 63
           M+ +V+G    + +DYEDPPP  F DA EL +WS YRA+IAEFVATLLFLYI VLTVIG 
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 64  ---NSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 123
              + + +    CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAV
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAV 120

Query: 124 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 183
           TFG+FLARK+SLIRA  Y+VAQCLGA+CG    K+ Q +YY +Y G AN +ADGY+ GTG
Sbjct: 121 TFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTG 180

Query: 184 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 243
           LAAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPA
Sbjct: 181 LAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPA 240

Query: 244 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           RSFGAA+IYNK+K WD HWIFW+GPFIGAAIAA YH  ++RA   K+L SFRS++
Sbjct: 241 RSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 283

BLAST of Lsi01G013460 vs. ExPASy Swiss-Prot
Match: P43286 (Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 2.9e-109
Identity = 201/284 (70.77%), Postives = 229/284 (80.63%), Query Frame = 0

Query: 13  KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSSLSAANT--- 72
           + +DY+DPPPA FID  EL +WSFYRA+IAEFVATLLFLYI VLTVIG    S  +    
Sbjct: 14  QTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGV 73

Query: 73  -CGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFLARKIS 132
            CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAVTFG+FLARK+S
Sbjct: 74  DCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAVTFGLFLARKVS 133

Query: 133 LIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEIIGTFVL 192
           L RA  YI+AQCLGA+CG    K+ Q +YY +Y G AN +ADGYS GTGLAAEIIGTFVL
Sbjct: 134 LPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVL 193

Query: 193 VYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAAMIYNK 252
           VYTVFSATDPKR+ARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPARSFGAA+IYNK
Sbjct: 194 VYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNK 253

Query: 253 AKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           +K WD HWIFW+GPFIGAAIAA YH  ++RA   K+L SFRS++
Sbjct: 254 SKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 285

BLAST of Lsi01G013460 vs. ExPASy Swiss-Prot
Match: P30302 (Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 2.5e-108
Identity = 200/295 (67.80%), Postives = 234/295 (79.32%), Query Frame = 0

Query: 4   MSNNVDGRR--KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG- 63
           M+ +V+G    + +DYEDPPP  F DA EL +WS YRA+IAEFVATLLFLY+ VLTVIG 
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 64  ---NSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 123
              + + +    CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAV
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAV 120

Query: 124 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 183
           TFG+FLARK+SLIRA  Y+VAQCLGA+CG    K+ Q ++YV Y G AN +ADGY+ GTG
Sbjct: 121 TFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTG 180

Query: 184 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 243
           LAAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPA
Sbjct: 181 LAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPA 240

Query: 244 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           RSFGAA+I+NK+K WD HWIFW+GPFIGA IAA YH  ++RA   K+L SFRS++
Sbjct: 241 RSFGAAVIFNKSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAA 283

BLAST of Lsi01G013460 vs. ExPASy Swiss-Prot
Match: Q8H5N9 (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.2e-108
Identity = 204/294 (69.39%), Postives = 230/294 (78.23%), Query Frame = 0

Query: 5   SNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG---- 64
           S    G    KDY DPPPA  IDA EL  WS YRA+IAEF+ATLLFLYI V TVIG    
Sbjct: 9   SGGAAGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQ 68

Query: 65  -NSSLSAAN-TCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVT 124
            ++S S A+  CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAVT
Sbjct: 69  TDASASGADAACGGVGVLGIAWAFGGMIFILVYCTAGIS------------GGHINPAVT 128

Query: 125 FGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGL 184
           FG+FLARK+SL+RA  YIVAQCLGA+CG  L K+ Q  Y+ +Y G AN +A GYS GTGL
Sbjct: 129 FGLFLARKVSLVRAILYIVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSKGTGL 188

Query: 185 AAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPAR 244
           AAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPAR
Sbjct: 189 AAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPAR 248

Query: 245 SFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           S GAA+I+N  KAW +HWIFW+GPF+GAAIAA YH  I+RAG IKAL SFRS++
Sbjct: 249 SIGAAVIFNNEKAWHNHWIFWVGPFVGAAIAAFYHQYILRAGAIKALGSFRSNA 290

BLAST of Lsi01G013460 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 392.1 bits (1006), Expect = 5.5e-108
Identity = 203/289 (70.24%), Postives = 227/289 (78.55%), Query Frame = 0

Query: 10  GRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG-----NSSL 69
           G    KDY DPPPA  IDA EL  WS YRA+IAEF+ATLLFLYI V TVIG     ++S 
Sbjct: 14  GEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASA 73

Query: 70  SAAN-TCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 129
           S A+  CGG+G LGI+WA GGMIFVLVYCTAGIS            GGHINPAVTFG+FL
Sbjct: 74  SGADAACGGVGVLGIAWAFGGMIFVLVYCTAGIS------------GGHINPAVTFGLFL 133

Query: 130 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 189
           ARK+SL+RA  YIVAQCLGA+CG  L K+ Q  Y+ +Y G AN +A GYS GTGL AEII
Sbjct: 134 ARKVSLVRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEII 193

Query: 190 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 249
           GTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIP+TGTGINPARS GAA
Sbjct: 194 GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAA 253

Query: 250 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           +IYNK K WD HWIFW+GP +GAAIAA YH  I+RAG IKAL SFRS++
Sbjct: 254 VIYNKDKPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRSNA 290

BLAST of Lsi01G013460 vs. ExPASy TrEMBL
Match: A0A0A0KEN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445150 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.9e-132
Identity = 246/291 (84.54%), Postives = 261/291 (89.69%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA FID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPFIDSDEFTQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFG+ L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGLLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RAF YI+AQCLGA+CGC LAKSLQKTYYVQY GAANMVA+ YSIGTGLAAEII
Sbjct: 121 ARKISLVRAFSYILAQCLGAICGCGLAKSLQKTYYVQYNGAANMVANEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAV +VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVTMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAALYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. ExPASy TrEMBL
Match: A0A5A7U0Z3 (Aquaporin PIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00540 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.6e-132
Identity = 244/291 (83.85%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA  ID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPLIDSDEFSQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFGM L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGMLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RA  YI+AQCLGA+CGC LAKSLQ+TYYVQY GAANMV+D YSIGTGLAAEII
Sbjct: 121 ARKISLVRALSYILAQCLGAICGCGLAKSLQQTYYVQYNGAANMVSDEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAVI+VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVIMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAAIYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. ExPASy TrEMBL
Match: A0A1S3BM85 (aquaporin PIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103491187 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.6e-132
Identity = 244/291 (83.85%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA  ID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPLIDSDEFSQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFGM L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGMLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RA  YI+AQCLGA+CGC LAKSLQ+TYYVQY GAANMV+D YSIGTGLAAEII
Sbjct: 121 ARKISLVRALSYILAQCLGAICGCGLAKSLQQTYYVQYNGAANMVSDEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAVI+VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVIMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAAIYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. ExPASy TrEMBL
Match: V5RDM0 (Plasma intrinsic protein 2-6 OS=Cucumis sativus OX=3659 GN=PIP2-6 PE=2 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.1e-131
Identity = 245/291 (84.19%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA FID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPFIDSDEFTQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLV CTAGIS            GGHINPAVTFG+ L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVCCTAGIS------------GGHINPAVTFGLLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RAF YI+AQCLGA+CGC LAKSLQKTYYVQY GAANMVA+ YSIGTGLAAEII
Sbjct: 121 ARKISLVRAFSYILAQCLGAICGCGLAKSLQKTYYVQYNGAANMVANEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAV +VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVTMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAALYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. ExPASy TrEMBL
Match: A0A6J1EDX4 (probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111433284 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.6e-121
Identity = 226/297 (76.09%), Postives = 249/297 (83.84%), Query Frame = 0

Query: 5   SNNVDGRRK-------VKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLT 64
           SN V  RR         KDY+DPPPA  IDAGE  QWSFYRAI  EFVATLLFLY+LVLT
Sbjct: 3   SNTVFERRSNGGLAATAKDYQDPPPAPLIDAGEFTQWSFYRAITVEFVATLLFLYVLVLT 62

Query: 65  VIGNSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 124
           VIG  + SA N CGG+G LGI+WAVGGMIFVLVYCTAGIS            GGHINPAV
Sbjct: 63  VIGYDTQSATNVCGGVGVLGIAWAVGGMIFVLVYCTAGIS------------GGHINPAV 122

Query: 125 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 184
           TFG+FLARKISLIRA FYI+AQCLGA+CGCALAKS QK YYV+Y+G  N++ADGYSI TG
Sbjct: 123 TFGLFLARKISLIRAVFYIMAQCLGAICGCALAKSFQKAYYVRYRGGTNILADGYSITTG 182

Query: 185 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 244
           LAAEI GTF+LVYTVFSATDPKRNARDSH+PVLAPLPIGFAV IVHLATIPITGTGINPA
Sbjct: 183 LAAEIAGTFILVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPA 242

Query: 245 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           RSFGAA+IYN+ +AWDHHWIFW+GPF+GAAIAA+YH VIIRAG +KAL SF++SSAL
Sbjct: 243 RSFGAAVIYNRTEAWDHHWIFWVGPFVGAAIAAIYHQVIIRAGAVKALGSFKTSSAL 287

BLAST of Lsi01G013460 vs. NCBI nr
Match: XP_038881301.1 (aquaporin PIP2-1-like [Benincasa hispida])

HSP 1 Score: 485.7 bits (1249), Expect = 2.8e-133
Identity = 252/291 (86.60%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MS NVDGR   KDY+DPPPA FI AGE  QWSFYRAIIAEFVATLLFLYILVLTVIGNS 
Sbjct: 1   MSKNVDGRSNGKDYQDPPPAPFIGAGEFNQWSFYRAIIAEFVATLLFLYILVLTVIGNSH 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LSA   CGG+G LGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFGM L
Sbjct: 61  LSALKICGGVGDLGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGMLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISLIRA FYI AQCLGA+CGCALAKSLQKTYYVQY GAAN+VADGYSIGTGLAAEII
Sbjct: 121 ARKISLIRALFYIFAQCLGAICGCALAKSLQKTYYVQYNGAANVVADGYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVI+VHLATIPITGTGINPARSFGAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIMVHLATIPITGTGINPARSFGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +IYNKAKAWDH WIFW+GPFIGAAIAA+YHVVIIRAG IKALVSFRSSSAL
Sbjct: 241 VIYNKAKAWDHQWIFWVGPFIGAAIAAIYHVVIIRAGAIKALVSFRSSSAL 279

BLAST of Lsi01G013460 vs. NCBI nr
Match: XP_004143246.1 (aquaporin PIP2-1 [Cucumis sativus] >KGN48150.1 hypothetical protein Csa_004273 [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 4.0e-132
Identity = 246/291 (84.54%), Postives = 261/291 (89.69%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA FID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPFIDSDEFTQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFG+ L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGLLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RAF YI+AQCLGA+CGC LAKSLQKTYYVQY GAANMVA+ YSIGTGLAAEII
Sbjct: 121 ARKISLVRAFSYILAQCLGAICGCGLAKSLQKTYYVQYNGAANMVANEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAV +VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVTMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAALYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. NCBI nr
Match: NP_001380712.1 (aquaporin PIP2-6 [Cucumis melo] >KAA0047261.1 aquaporin PIP2-1-like [Cucumis melo var. makuwa])

HSP 1 Score: 479.6 bits (1233), Expect = 2.0e-131
Identity = 244/291 (83.85%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA  ID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPLIDSDEFSQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLVYCTAGIS            GGHINPAVTFGM L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVYCTAGIS------------GGHINPAVTFGMLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RA  YI+AQCLGA+CGC LAKSLQ+TYYVQY GAANMV+D YSIGTGLAAEII
Sbjct: 121 ARKISLVRALSYILAQCLGAICGCGLAKSLQQTYYVQYNGAANMVSDEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAVI+VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVIMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAAIYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. NCBI nr
Match: AHB33482.1 (plasma intrinsic protein 2-6 [Cucumis sativus])

HSP 1 Score: 478.4 bits (1230), Expect = 4.4e-131
Identity = 245/291 (84.19%), Postives = 260/291 (89.35%), Query Frame = 0

Query: 4   MSNNVDGRRKVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSS 63
           MSNN+DGR  VKDY+DPPPA FID+ E  QWSFYRAIIAEFVATLLFLYILVLTVIGN+ 
Sbjct: 1   MSNNIDGRSNVKDYQDPPPAPFIDSDEFTQWSFYRAIIAEFVATLLFLYILVLTVIGNAR 60

Query: 64  LSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFL 123
           LS  N CGG+GALGISWAVGGMIFVLV CTAGIS            GGHINPAVTFG+ L
Sbjct: 61  LSDTNICGGVGALGISWAVGGMIFVLVCCTAGIS------------GGHINPAVTFGLLL 120

Query: 124 ARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEII 183
           ARKISL+RAF YI+AQCLGA+CGC LAKSLQKTYYVQY GAANMVA+ YSIGTGLAAEII
Sbjct: 121 ARKISLVRAFSYILAQCLGAICGCGLAKSLQKTYYVQYNGAANMVANEYSIGTGLAAEII 180

Query: 184 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAA 243
           GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAV +VHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVTMVHLATIPITGTGINPARSLGAA 240

Query: 244 MIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           +I+NKAKAWDHHWIFW+GPFIGAAIAA+YHVVIIRAGTIKAL SFRSSSAL
Sbjct: 241 VIFNKAKAWDHHWIFWVGPFIGAAIAALYHVVIIRAGTIKALASFRSSSAL 279

BLAST of Lsi01G013460 vs. NCBI nr
Match: XP_023543954.1 (aquaporin PIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 447.2 bits (1149), Expect = 1.1e-121
Identity = 228/297 (76.77%), Postives = 251/297 (84.51%), Query Frame = 0

Query: 5   SNNVDGRRK-------VKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLT 64
           SN+V  RR         KDY+DPPPA FIDAGE  QWSFYRAI  EFVATLLFLYILVLT
Sbjct: 3   SNSVFERRSNGGLAATAKDYQDPPPAPFIDAGEFTQWSFYRAITVEFVATLLFLYILVLT 62

Query: 65  VIGNSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 124
           VIG  + SA N CGG+G LGI+WAVGGMIFVLVYCTAGIS            GGHINPAV
Sbjct: 63  VIGYDTQSATNVCGGVGVLGIAWAVGGMIFVLVYCTAGIS------------GGHINPAV 122

Query: 125 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 184
           TFG+FLARKISLIRA FYI+AQCLGA+CGCALAKS QK YYV+Y+G  N++ADGYSI TG
Sbjct: 123 TFGLFLARKISLIRAVFYIMAQCLGAICGCALAKSFQKGYYVRYRGGTNILADGYSITTG 182

Query: 185 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 244
           LAAEI GTF+LVYTVFSATDPKRNARDSH+PVLAPLPIGFAV IVHLATIPITGTGINPA
Sbjct: 183 LAAEIAGTFILVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPA 242

Query: 245 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSSAL 295
           RSFGAA+IYN+ +AWDHHWIFW+GPF+GAAIAA+YH VIIRAG +KAL SF++SSAL
Sbjct: 243 RSFGAAVIYNRTEAWDHHWIFWVGPFVGAAIAAIYHQVIIRAGAVKALGSFKTSSAL 287

BLAST of Lsi01G013460 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 397.1 bits (1019), Expect = 1.2e-110
Identity = 203/295 (68.81%), Postives = 235/295 (79.66%), Query Frame = 0

Query: 4   MSNNVDGRR--KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG- 63
           M+ +V+G    + +DYEDPPP  F DA EL +WS YRA+IAEFVATLLFLYI VLTVIG 
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 64  ---NSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 123
              + + +    CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAV
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAV 120

Query: 124 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 183
           TFG+FLARK+SLIRA  Y+VAQCLGA+CG    K+ Q +YY +Y G AN +ADGY+ GTG
Sbjct: 121 TFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTG 180

Query: 184 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 243
           LAAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPA
Sbjct: 181 LAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPA 240

Query: 244 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           RSFGAA+IYNK+K WD HWIFW+GPFIGAAIAA YH  ++RA   K+L SFRS++
Sbjct: 241 RSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 283

BLAST of Lsi01G013460 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 396.4 bits (1017), Expect = 2.1e-110
Identity = 201/284 (70.77%), Postives = 229/284 (80.63%), Query Frame = 0

Query: 13  KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSSLSAANT--- 72
           + +DY+DPPPA FID  EL +WSFYRA+IAEFVATLLFLYI VLTVIG    S  +    
Sbjct: 14  QTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGV 73

Query: 73  -CGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFLARKIS 132
            CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAVTFG+FLARK+S
Sbjct: 74  DCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAVTFGLFLARKVS 133

Query: 133 LIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEIIGTFVL 192
           L RA  YI+AQCLGA+CG    K+ Q +YY +Y G AN +ADGYS GTGLAAEIIGTFVL
Sbjct: 134 LPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVL 193

Query: 193 VYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAAMIYNK 252
           VYTVFSATDPKR+ARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPARSFGAA+IYNK
Sbjct: 194 VYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNK 253

Query: 253 AKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           +K WD HWIFW+GPFIGAAIAA YH  ++RA   K+L SFRS++
Sbjct: 254 SKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 285

BLAST of Lsi01G013460 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 396.4 bits (1017), Expect = 2.1e-110
Identity = 201/284 (70.77%), Postives = 229/284 (80.63%), Query Frame = 0

Query: 13  KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSSLSAANT--- 72
           + +DY+DPPPA FID  EL +WSFYRA+IAEFVATLLFLYI VLTVIG    S  +    
Sbjct: 14  QTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGV 73

Query: 73  -CGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFLARKIS 132
            CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAVTFG+FLARK+S
Sbjct: 74  DCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAVTFGLFLARKVS 133

Query: 133 LIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEIIGTFVL 192
           L RA  YI+AQCLGA+CG    K+ Q +YY +Y G AN +ADGYS GTGLAAEIIGTFVL
Sbjct: 134 LPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVL 193

Query: 193 VYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAAMIYNK 252
           VYTVFSATDPKR+ARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPARSFGAA+IYNK
Sbjct: 194 VYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNK 253

Query: 253 AKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           +K WD HWIFW+GPFIGAAIAA YH  ++RA   K+L SFRS++
Sbjct: 254 SKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 285

BLAST of Lsi01G013460 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 1.7e-109
Identity = 200/295 (67.80%), Postives = 234/295 (79.32%), Query Frame = 0

Query: 4   MSNNVDGRR--KVKDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIG- 63
           M+ +V+G    + +DYEDPPP  F DA EL +WS YRA+IAEFVATLLFLY+ VLTVIG 
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 64  ---NSSLSAANTCGGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAV 123
              + + +    CGG+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAV
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGIS------------GGHINPAV 120

Query: 124 TFGMFLARKISLIRAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTG 183
           TFG+FLARK+SLIRA  Y+VAQCLGA+CG    K+ Q ++YV Y G AN +ADGY+ GTG
Sbjct: 121 TFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTG 180

Query: 184 LAAEIIGTFVLVYTVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPA 243
           LAAEIIGTFVLVYTVFSATDPKRNARDSH+PVLAPLPIGFAV +VHLATIPITGTGINPA
Sbjct: 181 LAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPA 240

Query: 244 RSFGAAMIYNKAKAWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRSSS 293
           RSFGAA+I+NK+K WD HWIFW+GPFIGA IAA YH  ++RA   K+L SFRS++
Sbjct: 241 RSFGAAVIFNKSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAA 283

BLAST of Lsi01G013460 vs. TAIR 10
Match: AT3G54820.1 (plasma membrane intrinsic protein 2;5 )

HSP 1 Score: 391.7 bits (1005), Expect = 5.1e-109
Identity = 197/280 (70.36%), Postives = 227/280 (81.07%), Query Frame = 0

Query: 15  KDYEDPPPALFIDAGELIQWSFYRAIIAEFVATLLFLYILVLTVIGNSSLS----AANTC 74
           KDY+DPPP    DA EL +WSFYRA+IAEF+ATLLFLY+ ++TVIG  S +      + C
Sbjct: 15  KDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDPALNPDQC 74

Query: 75  GGIGALGISWAVGGMIFVLVYCTAGISDLSNYAVLFISVGGHINPAVTFGMFLARKISLI 134
            G+G LGI+WA GGMIF+LVYCTAGIS            GGHINPAVTFG+ LARK++L+
Sbjct: 75  TGVGVLGIAWAFGGMIFILVYCTAGIS------------GGHINPAVTFGLLLARKVTLV 134

Query: 135 RAFFYIVAQCLGAVCGCALAKSLQKTYYVQYQGAANMVADGYSIGTGLAAEIIGTFVLVY 194
           RA  Y+VAQCLGA+CG AL K+ Q  Y+ +Y G AN ++DGYSIGTG+AAEIIGTFVLVY
Sbjct: 135 RAVMYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVY 194

Query: 195 TVFSATDPKRNARDSHIPVLAPLPIGFAVIIVHLATIPITGTGINPARSFGAAMIYNKAK 254
           TVFSATDPKR+ARDSH+PVLAPLPIGFAV IVHLATIPITGTGINPARS GAA+IYNK K
Sbjct: 195 TVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDK 254

Query: 255 AWDHHWIFWIGPFIGAAIAAVYHVVIIRAGTIKALVSFRS 291
           AWDHHWIFW+GPF GAAIAA YH  ++RAG IKAL SFRS
Sbjct: 255 AWDHHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRS 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P432871.7e-10968.81Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2[more]
P432862.9e-10970.77Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1[more]
P303022.5e-10867.80Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1[more]
Q8H5N93.2e-10869.39Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=... [more]
Q84RL75.5e-10870.24Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KEN61.9e-13284.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445150 PE=3 SV=1[more]
A0A5A7U0Z39.6e-13283.85Aquaporin PIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold90... [more]
A0A1S3BM859.6e-13283.85aquaporin PIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103491187 PE=3 SV=1[more]
V5RDM02.1e-13184.19Plasma intrinsic protein 2-6 OS=Cucumis sativus OX=3659 GN=PIP2-6 PE=2 SV=1[more]
A0A6J1EDX42.6e-12176.09probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111433284 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_038881301.12.8e-13386.60aquaporin PIP2-1-like [Benincasa hispida][more]
XP_004143246.14.0e-13284.54aquaporin PIP2-1 [Cucumis sativus] >KGN48150.1 hypothetical protein Csa_004273 [... [more]
NP_001380712.12.0e-13183.85aquaporin PIP2-6 [Cucumis melo] >KAA0047261.1 aquaporin PIP2-1-like [Cucumis mel... [more]
AHB33482.14.4e-13184.19plasma intrinsic protein 2-6 [Cucumis sativus][more]
XP_023543954.11.1e-12176.77aquaporin PIP2-1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G37170.11.2e-11068.81plasma membrane intrinsic protein 2 [more]
AT3G53420.12.1e-11070.77plasma membrane intrinsic protein 2A [more]
AT3G53420.22.1e-11070.77plasma membrane intrinsic protein 2A [more]
AT2G37180.11.7e-10967.80Aquaporin-like superfamily protein [more]
AT3G54820.15.1e-10970.36plasma membrane intrinsic protein 2;5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 256..276
score: 46.52
coord: 180..198
score: 54.14
coord: 216..238
score: 56.46
coord: 94..118
score: 35.65
coord: 131..150
score: 47.22
coord: 38..57
score: 43.43
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 42..272
e-value: 5.3E-56
score: 187.9
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 31..273
e-value: 2.4E-72
score: 243.3
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 38..276
e-value: 2.21674E-68
score: 210.188
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 24..294
e-value: 6.9E-78
score: 263.2
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 30..278
NoneNo IPR availablePANTHERPTHR45687:SF66AQUAPORIN PIP2-1coord: 4..291
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 4..291
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 112..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G013460.1Lsi01G013460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity