Lsi01G012960 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G012960
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
Locationchr01: 11237794 .. 11240121 (+)
RNA-Seq ExpressionLsi01G012960
SyntenyLsi01G012960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCGTTTTCCCCTCCTTTCGCTTTCCAATTTGCACCATGTGAACCCAACTTCCAGTCCAAAGTCCACTGCAATTGCAACTTAGCAAAATATCTTCTCTCAATCACATCTTCCATTTCTCTATAAAAAGCTTCAATTCAATGCCTTTGCAAGCATGAGAGGCACAAGATTCAGCATCAGAACTGGGCGAAGAAGAATGTCACAGATGACGAATTTCGCCTTCCAAGTTCTATTCGGCCGATGGTTCACCGTCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGCGCCACTTACATGTTCGCACTCTACTCCGCCGACATCAAATCCTCCTTCAATTACGATCAGACCACTCTCAACCTCATCGGCTTCTTTAAGGATCTGGGTTCCAACGTCGGAGTCTTTTCCGGCCTCATCAATGAAATTACTCCACCTTGGGTCGTCCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTTTCTGTCACTCACCGAATCCCCAAACCCAAACTGCCCGCCATGTGTTTGCTTATATGTCTAGGTGCCAATTCTCAGACATTTTCTAATACAGGGGCTTTGATCCCTTCTGTGAGGAATTTTCCCCAGAATCGTGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATTTTGACTCAGATTTACCATGCTTTTTATGGGGATGATTCTAAGGATTTTATCTTGCTTATTGCTTGGTTGCCTACTGCTGTTTCTTTCCTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTCAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTTGCTGGCTTCCTCATGATTTTGATTATTATTCAAAATGAACTCATGTTTACTAGAATTCAGTATTTGGGTTGTGCGTTTGTTCTCCTAGCGTTTCTTTTTCTTCCCATTGTCGTTGTTATAAGAGAAGAATTCAGCATTAGGAAGAGGAAATTGCAAGGTGTGGATGTAACTTCTTGGCTTCCAATTCCACCAGACCCATCTCGAGCTGAATTACCATCGCCTAGAACTTCAAGTGTGCCGACAACTGCCATAGCTTTAGCCAACCCAAGTTCTTGCTTTGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAACCTTCAGTGTAGATATGTTGATTCTGTTTGTTGTCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATGGACAACTTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGTTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACAAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTCTTGTGCTTCTTCTCTCTTGCGTTGGTCATCTTCTAATTGCCTTTGGAGTCCCAACCTCCCTTTATTTTGCTTCAGTGATCATTGGATTTTGCTTTGGAGCACTATGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATAAGCGGCATTGCTAGCCCAGTTGGCTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATGGCAGCCATTGGTCTTAGAAGTGTAGCTGGTGGAGACTTAGACTGCAAGGGTGTCCATTGTTACAGGTTAGCTTTTCTCATAATCTCTGCTGCAACCGTGTTCGGTTGTGTTGTTTCATTCATTTTGGTCCTTCGAACTTGGAAATTCTATAAGGGTGATATCTATAAGAAGTTTAGAGAGGAAAGAAAGGAAGCAGAATGATCGAGTTAGTATTCCTGCTATAGGCTTACTTCTTTTGGCACTCTACAATGCCAGTTTGTGTAACACTGTTTGTTTCAACACGCTTCAGGACCTCGTTATTTTCCTTCTGTTCCGACATCTGAGAAAGAAGCGATTTCAGGATTGTGAAAAGATTGAAAGTTGTTCTAGTGTTGTAAGCTTTGTGAAAGGTAGAATGTTGCCTTTTGTTTTGGCAAAGATGGAGGGTAAAATCTTCATGTGTCTTTTTTGCTACGATCCAAATACGAAACCGAACTTGTCGTGCTGCTTGTGCACAGACGATCAGGAGTGGTGAATTGCCATTTTGCTCAGAAACCACAACCTATGGAGTAGTTGTCCAGTGTTTACTTTTGTTCAATGTGGAGATTGCTTACCTTAGTTCTATCAGAAGCTTTCCTCTTTTGCTTATCAGTTAG

mRNA sequence

TTTTCGTTTTCCCCTCCTTTCGCTTTCCAATTTGCACCATGTGAACCCAACTTCCAGTCCAAAGTCCACTGCAATTGCAACTTAGCAAAATATCTTCTCTCAATCACATCTTCCATTTCTCTATAAAAAGCTTCAATTCAATGCCTTTGCAAGCATGAGAGGCACAAGATTCAGCATCAGAACTGGGCGAAGAAGAATGTCACAGATGACGAATTTCGCCTTCCAAGTTCTATTCGGCCGATGGTTCACCGTCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGCGCCACTTACATGTTCGCACTCTACTCCGCCGACATCAAATCCTCCTTCAATTACGATCAGACCACTCTCAACCTCATCGGCTTCTTTAAGGATCTGGGTTCCAACGTCGGAGTCTTTTCCGGCCTCATCAATGAAATTACTCCACCTTGGGTCGTCCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTTTCTGTCACTCACCGAATCCCCAAACCCAAACTGCCCGCCATGTGTTTGCTTATATGTCTAGGTGCCAATTCTCAGACATTTTCTAATACAGGGGCTTTGATCCCTTCTGTGAGGAATTTTCCCCAGAATCGTGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATTTTGACTCAGATTTACCATGCTTTTTATGGGGATGATTCTAAGGATTTTATCTTGCTTATTGCTTGGTTGCCTACTGCTGTTTCTTTCCTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTCAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTTGCTGGCTTCCTCATGATTTTGATTATTATTCAAAATGAACTCATGTTTACTAGAATTCAGTATTTGGGTTGTGCGTTTGTTCTCCTAGCGTTTCTTTTTCTTCCCATTGTCGTTGTTATAAGAGAAGAATTCAGCATTAGGAAGAGGAAATTGCAAGGTGTGGATGTAACTTCTTGGCTTCCAATTCCACCAGACCCATCTCGAGCTGAATTACCATCGCCTAGAACTTCAAGTGTGCCGACAACTGCCATAGCTTTAGCCAACCCAAGTTCTTGCTTTGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAACCTTCAGTGTAGATATGTTGATTCTGTTTGTTGTCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATGGACAACTTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGTTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACAAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTCTTGTGCTTCTTCTCTCTTGCGTTGGTCATCTTCTAATTGCCTTTGGAGTCCCAACCTCCCTTTATTTTGCTTCAGTGATCATTGGATTTTGCTTTGGAGCACTATGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATAAGCGGCATTGCTAGCCCAGTTGGCTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATGGCAGCCATTGGTCTTAGAAGTGTAGCTGGTGGAGACTTAGACTGCAAGGGTGTCCATTGTTACAGGGTGATATCTATAAGAAGTTTAGAGAGGAAAGAAAGGAAGCAGAATGATCGAGTTAGTATTCCTGCTATAGGCTTACTTCTTTTGGCACTCTACAATGCCAGTTTGTGTAACACTGTTTGTTTCAACACGCTTCAGGACCTCGTTATTTTCCTTCTGTTCCGACATCTGAGAAAGAAGCGATTTCAGGATTGTGAAAAGATTGAAAGTTGTTCTAGTGTTACGATCAGGAGTGGTGAATTGCCATTTTGCTCAGAAACCACAACCTATGGAGTAGTTGTCCAGTGTTTACTTTTGTTCAATGTGGAGATTGCTTACCTTAGTTCTATCAGAAGCTTTCCTCTTTTGCTTATCAGTTAG

Coding sequence (CDS)

ATGAGAGGCACAAGATTCAGCATCAGAACTGGGCGAAGAAGAATGTCACAGATGACGAATTTCGCCTTCCAAGTTCTATTCGGCCGATGGTTCACCGTCTTCGCTTCTTTACTCATCATGTCCGTTTCCGGCGCCACTTACATGTTCGCACTCTACTCCGCCGACATCAAATCCTCCTTCAATTACGATCAGACCACTCTCAACCTCATCGGCTTCTTTAAGGATCTGGGTTCCAACGTCGGAGTCTTTTCCGGCCTCATCAATGAAATTACTCCACCTTGGGTCGTCCTCTTCATCGGTGGGGTAATGAATTTCTTTGGCTATTTCATGATTTGGCTTTCTGTCACTCACCGAATCCCCAAACCCAAACTGCCCGCCATGTGTTTGCTTATATGTCTAGGTGCCAATTCTCAGACATTTTCTAATACAGGGGCTTTGATCCCTTCTGTGAGGAATTTTCCCCAGAATCGTGGTAATGTATTGGGTTTATTGAAAGGGTTTGTTGGGTTAAGTGGGGCAATTTTGACTCAGATTTACCATGCTTTTTATGGGGATGATTCTAAGGATTTTATCTTGCTTATTGCTTGGTTGCCTACTGCTGTTTCTTTCCTGCTTCTTCGGATTGTTAGAGTAGTGGAAGTGAACCCAATATTCAAATCCAATGATCTCAAAAATTTCTATTCTATGCTCTACATTTCCCTTGGTCTTGCTGGCTTCCTCATGATTTTGATTATTATTCAAAATGAACTCATGTTTACTAGAATTCAGTATTTGGGTTGTGCGTTTGTTCTCCTAGCGTTTCTTTTTCTTCCCATTGTCGTTGTTATAAGAGAAGAATTCAGCATTAGGAAGAGGAAATTGCAAGGTGTGGATGTAACTTCTTGGCTTCCAATTCCACCAGACCCATCTCGAGCTGAATTACCATCGCCTAGAACTTCAAGTGTGCCGACAACTGCCATAGCTTTAGCCAACCCAAGTTCTTGCTTTGAAAACGTGTTCAGACCGCCGGAAAGAGGAGAGGACTACACCATTTTACAAGCAACCTTCAGTGTAGATATGTTGATTCTGTTTGTTGTCACAATTTGTGGGGCTGGTGGGACTTTGACTGCAATGGACAACTTGGGTCAGATAGGCAGCTCCCTTGGCTACTCAACTCATACCATTTCTACTTTCACTTCTCTAGTGAGTATATGGGGTTTTCTCGGTCGAGCATTTTCGGGTTATGCTTCTGAATTCTTATGGACAAAGTATAACTTCTCTCGCCCTTTGTTCCTCACTCTTGTGCTTCTTCTCTCTTGCGTTGGTCATCTTCTAATTGCCTTTGGAGTCCCAACCTCCCTTTATTTTGCTTCAGTGATCATTGGATTTTGCTTTGGAGCACTATGGCCATTGATTTTCGCCATTGTTTCTGAATTATTTGGCTTGAAATACTATGCAACTTTATATAGCATAAGCGGCATTGCTAGCCCAGTTGGCTCATACATTTTCAATGTGAAAGTAGCTGGCTATTTATACGACCAAGAGGCTAGAAAGCAAATGGCAGCCATTGGTCTTAGAAGTGTAGCTGGTGGAGACTTAGACTGCAAGGGTGTCCATTGTTACAGGGTGATATCTATAAGAAGTTTAGAGAGGAAAGAAAGGAAGCAGAATGATCGAGTTAGTATTCCTGCTATAGGCTTACTTCTTTTGGCACTCTACAATGCCAGTTTGTGTAACACTGTTTGTTTCAACACGCTTCAGGACCTCGTTATTTTCCTTCTGTTCCGACATCTGAGAAAGAAGCGATTTCAGGATTGTGAAAAGATTGAAAGTTGTTCTAGTGTTACGATCAGGAGTGGTGAATTGCCATTTTGCTCAGAAACCACAACCTATGGAGTAGTTGTCCAGTGTTTACTTTTGTTCAATGTGGAGATTGCTTACCTTAGTTCTATCAGAAGCTTTCCTCTTTTGCTTATCAGTTAG

Protein sequence

MRGTRFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRVISIRSLERKERKQNDRVSIPAIGLLLLALYNASLCNTVCFNTLQDLVIFLLFRHLRKKRFQDCEKIESCSSVTIRSGELPFCSETTTYGVVVQCLLLFNVEIAYLSSIRSFPLLLIS
Homology
BLAST of Lsi01G012960 vs. ExPASy TrEMBL
Match: A0A1S3CH39 (uncharacterized protein LOC103500834 OS=Cucumis melo OX=3656 GN=LOC103500834 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.8e-271
Identity = 485/532 (91.17%), Postives = 502/532 (94.36%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL I LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+ TT IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLLLSC GHLLI+ GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. ExPASy TrEMBL
Match: A0A5D3C849 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00430 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 3.1e-271
Identity = 484/532 (90.98%), Postives = 501/532 (94.17%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL + LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFVFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+ TT IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLL SC GHLLIA GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. ExPASy TrEMBL
Match: A0A5A7V9Y5 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00110 PE=4 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 1.2e-270
Identity = 484/532 (90.98%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL I LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+  T IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRMTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLL SC GHLLIA GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. ExPASy TrEMBL
Match: A0A6J1CR80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111013589 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.7e-250
Identity = 455/536 (84.89%), Postives = 485/536 (90.49%), Query Frame = 0

Query: 1   MRGTRFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSF 60
           M G R  I +G  R  QM NFA QVL GRWFTVFASLLIM+VSGATYMF LYSADIKSS 
Sbjct: 1   MGGARLRIWSGSGR-RQMRNFALQVLLGRWFTVFASLLIMAVSGATYMFGLYSADIKSSL 60

Query: 61  NYDQTTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP 120
            YDQTTLNL+GFFKDLG+NVGV SGLINEITPPWVVLFIGGVMNFFGYFMIWLSVT RI 
Sbjct: 61  GYDQTTLNLLGFFKDLGANVGVLSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTQRIA 120

Query: 121 KPKLPAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180
           KPK+PAMCL ICLGANSQT++NTGALIPSV+NFP+ RGNVLGLLKGFVGLSGAILTQ+YH
Sbjct: 121 KPKVPAMCLYICLGANSQTYANTGALIPSVKNFPEYRGNVLGLLKGFVGLSGAILTQLYH 180

Query: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL 240
           AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEV+   KSNDLK FY +LYISLGLA FL
Sbjct: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVDQRSKSNDLKIFYHLLYISLGLACFL 240

Query: 241 MILIIIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPP 300
           MILIIIQNEL+FTRIQYLGCA +LL  LFLP+ VVI EEFSI+KRKLQ ++VTSWL    
Sbjct: 241 MILIIIQNELVFTRIQYLGCALILLVLLFLPLAVVITEEFSIKKRKLQALNVTSWL---S 300

Query: 301 DPSRAELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVT 360
           DPS AELPS R   +PT  +A+ANPSSCFENVFRPPERGEDYTILQA FS+DMLILFV T
Sbjct: 301 DPSPAELPSSRPPHLPTAVVAVANPSSCFENVFRPPERGEDYTILQAIFSIDMLILFVAT 360

Query: 361 ICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS 420
           ICG GGTLTA+DNLGQIGSSLGYSTH+ISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS
Sbjct: 361 ICGTGGTLTAIDNLGQIGSSLGYSTHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS 420

Query: 421 RPLFLTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYAT 480
           RPLFLTLVLLLSC+GHLLIAFGVPTSLYFASVIIGFCFGA WPLIFAIVSELFGLKYYAT
Sbjct: 421 RPLFLTLVLLLSCIGHLLIAFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYAT 480

Query: 481 LYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           LYSISGIASPVG+YIF+VKVAGYLYD+EAR+QMAAIGLR VA GDL CKGV CYR+
Sbjct: 481 LYSISGIASPVGTYIFSVKVAGYLYDEEARRQMAAIGLRRVA-GDLACKGVQCYRL 531

BLAST of Lsi01G012960 vs. ExPASy TrEMBL
Match: A0A6J1ECZ3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111433047 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 3.3e-249
Identity = 458/536 (85.45%), Postives = 481/536 (89.74%), Query Frame = 0

Query: 1   MRGTRFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSF 60
           M G RFSIRT R  +  M NF +QVL GRWFTVFASLLIMSVSGATYMFALYSADIKSS 
Sbjct: 1   MGGVRFSIRTLRSSV-PMRNFVWQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSL 60

Query: 61  NYDQTTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP 120
           +YDQTTLNLIGFFKDLG+NVGVFSGLINEITPPWVVL IGGVMNFFGYFMIWLSVTHRIP
Sbjct: 61  DYDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIP 120

Query: 121 KPKLPAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180
           KPKLPAMCL ICLGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYH
Sbjct: 121 KPKLPAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180

Query: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL 240
           AFYGDDSKDFILLIAWLPTAVSFLLLR+VRVVEVN I KS+DLKNFY MLY SL LAGFL
Sbjct: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSSDLKNFYYMLYTSLVLAGFL 240

Query: 241 MILIIIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPP 300
           MILIIIQNEL+FTRIQYLGCAF+LL  LFLP+VVVIREEF +RKRK Q +DV S LP+  
Sbjct: 241 MILIIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDVISVLPL-- 300

Query: 301 DPSRAELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVT 360
           DPS  EL S R             PSSCF+NVF+PPERG+DYTILQA FSVDMLILFV T
Sbjct: 301 DPSPVELSSSRP------------PSSCFKNVFKPPERGDDYTILQAIFSVDMLILFVAT 360

Query: 361 ICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS 420
           ICGAGG LTAMDNLGQIGSSLGYS H+ISTFTSLVSIWGFLGRAFSGYASEFLWTKY+FS
Sbjct: 361 ICGAGGALTAMDNLGQIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFS 420

Query: 421 RPLFLTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYAT 480
           RPLF TLVLL SCVGHLLIAFGVPTSLYF+SVIIGFCFGA WPLIFAIVSELFGLKYYAT
Sbjct: 421 RPLFFTLVLLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYAT 480

Query: 481 LYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           LYSISGIASPVGSYIFNVKVAG+LYDQEARKQMAA GL +VAG DL CKGVHCYR+
Sbjct: 481 LYSISGIASPVGSYIFNVKVAGFLYDQEARKQMAASGLVTVAGRDLACKGVHCYRL 521

BLAST of Lsi01G012960 vs. NCBI nr
Match: XP_038882767.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 959.5 bits (2479), Expect = 1.5e-275
Identity = 494/536 (92.16%), Postives = 507/536 (94.59%), Query Frame = 0

Query: 1   MRGTRFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSF 60
           M+  RFSIRTGRRR+S MTNF  QVL GRWFTVFASLLIMSVSGATYMFALYSADIKSS 
Sbjct: 1   MKSARFSIRTGRRRLSPMTNFVVQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSL 60

Query: 61  NYDQTTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP 120
           NYDQTTLNLIGFFKDLGSNVGVFSGLI EITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP
Sbjct: 61  NYDQTTLNLIGFFKDLGSNVGVFSGLITEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP 120

Query: 121 KPKLPAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180
           KPKLPAMCL ICLGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYH
Sbjct: 121 KPKLPAMCLFICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180

Query: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL 240
           AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL
Sbjct: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL 240

Query: 241 MILIIIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPP 300
           MILIIIQNELMFTRIQYLGCAFVLLAFLFLP+VVVIREEFS RKRKLQGVD+TSWLP+PP
Sbjct: 241 MILIIIQNELMFTRIQYLGCAFVLLAFLFLPLVVVIREEFSSRKRKLQGVDLTSWLPVPP 300

Query: 301 DPSRAELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVT 360
           DPS AELP PR S+         + SSCFENVFRPPERGEDYTILQA FSVDMLILFV T
Sbjct: 301 DPSPAELPLPRPSN---------SSSSCFENVFRPPERGEDYTILQAIFSVDMLILFVAT 360

Query: 361 ICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS 420
           ICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS
Sbjct: 361 ICGAGGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFS 420

Query: 421 RPLFLTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYAT 480
           RPLFLTL+LLLSCVGHLLI+FGVPTSLYFASVIIGFCFGA WPLIFAIVSELFGLKYYAT
Sbjct: 421 RPLFLTLLLLLSCVGHLLISFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYAT 480

Query: 481 LYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           LYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGL+SVAG DL C GVHCYR+
Sbjct: 481 LYSISGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLKSVAGRDLACVGVHCYRL 527

BLAST of Lsi01G012960 vs. NCBI nr
Match: XP_008462497.1 (PREDICTED: uncharacterized protein LOC103500834 [Cucumis melo])

HSP 1 Score: 944.9 bits (2441), Expect = 3.7e-271
Identity = 485/532 (91.17%), Postives = 502/532 (94.36%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL I LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+ TT IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLLLSC GHLLI+ GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLLSCFGHLLISSGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. NCBI nr
Match: TYK07328.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 944.1 bits (2439), Expect = 6.3e-271
Identity = 484/532 (90.98%), Postives = 501/532 (94.17%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL + LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFVFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+ TT IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLL SC GHLLIA GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. NCBI nr
Match: XP_004143363.2 (uncharacterized protein LOC101203981 [Cucumis sativus] >KAE8647411.1 hypothetical protein Csa_004435 [Cucumis sativus])

HSP 1 Score: 943.7 bits (2438), Expect = 8.3e-271
Identity = 486/531 (91.53%), Postives = 499/531 (93.97%), Query Frame = 0

Query: 6   FSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQT 65
           FSIRTG+RR SQM+NFA QVL GRWFTVFASLLIMSVSGATYMFALYS+DIKSS NYDQT
Sbjct: 14  FSIRTGQRRRSQMSNFAVQVLLGRWFTVFASLLIMSVSGATYMFALYSSDIKSSLNYDQT 73

Query: 66  TLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLP 125
           TLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLP
Sbjct: 74  TLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLP 133

Query: 126 AMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGD 185
           AMCL   LGANSQTF+NTGALIPSV+NFPQNRG VLGLLKGFVGLSGAILTQIYHAFYGD
Sbjct: 134 AMCLFTFLGANSQTFANTGALIPSVKNFPQNRGYVLGLLKGFVGLSGAILTQIYHAFYGD 193

Query: 186 DSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILII 245
           DSKDFILLIAWLPTAVS LLLRIVRVVE NP FKSNDLKNFYSMLYISLGLAGFLMILII
Sbjct: 194 DSKDFILLIAWLPTAVSLLLLRIVRVVEANPTFKSNDLKNFYSMLYISLGLAGFLMILII 253

Query: 246 IQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRA 305
           IQNELMFTRIQYLGC FVLL FLFLP+VV+IREEF IRKRKLQGVDVTSWLP+P D S  
Sbjct: 254 IQNELMFTRIQYLGCVFVLLTFLFLPLVVIIREEFGIRKRKLQGVDVTSWLPVPSDESPD 313

Query: 306 ELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAG 365
           ELP PRTSS PTT  ALANPSSCFENVFRPPERGEDYTILQA FSVDMLILF VTICGAG
Sbjct: 314 ELPLPRTSSFPTTDTALANPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFVTICGAG 373

Query: 366 GTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFL 425
           GTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFL
Sbjct: 374 GTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFL 433

Query: 426 TLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSIS 485
           TLVLLLSC GHLLIA G+PTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSIS
Sbjct: 434 TLVLLLSCFGHLLIASGLPTSVYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSIS 493

Query: 486 GIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           GIASPVGSYIFNVKVAGYLYDQEARKQM   GLR+VAG DL CKGVHCYR+
Sbjct: 494 GIASPVGSYIFNVKVAGYLYDQEARKQM-DFGLRNVAGRDLACKGVHCYRL 543

BLAST of Lsi01G012960 vs. NCBI nr
Match: KAA0063797.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 942.2 bits (2434), Expect = 2.4e-270
Identity = 484/532 (90.98%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 5   RFSIRTGRRRMSQMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQ 64
           RFSI TG+RR SQM+NFA QVL GRWFT FASLLIMSVSGA+YMFALYS+DIKSS NYDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 184
           PAMCL I LGANSQTF+NTGALIPSV+NFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 244
           DDSKDFILLIAWLPTAVS LLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSR 304
           IIQNELMFTRIQYL C FVLLAFLFLP+VVVIREEFSIRKRKLQGVDVTSWLP+P D S 
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 AELPSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGA 364
           AELP PR SS+  T IAL NPSSCFENVFRPPERGEDYTILQA FSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRMTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGTLTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 424
           GGTLTA+DNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 LTLVLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSI 484
           LT VLL SC GHLLIA GVPTS+YFASVIIGFCFGA WPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           SGIASP+GSYIFNVKVAGYLYDQEARKQ+ AIGLR+VAG DL CKGVHCYR+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRL 544

BLAST of Lsi01G012960 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 599.4 bits (1544), Expect = 3.6e-171
Identity = 308/523 (58.89%), Postives = 384/523 (73.42%), Query Frame = 0

Query: 18  MTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDLG 77
           M +   Q+L GRWF  F SLLIMS +GATYMF +YS DIK +  YDQTTLNL+ FFKDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 78  SNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLLICLGANS 137
           +NVGV +GL+NE+TPPW +L IG ++NFFGYFMIWL+VT RI KP++  MCL IC+GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 138 QTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAWL 197
           Q+F+NTG+L+  V+NFP++RG VLG+LKG+VGLSGAI+TQ+Y AFYG+D+K+ IL+I WL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

Query: 198 PTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQY 257
           P  VSF  LR +R+++V    ++N+LK FY+ LYISLGLA FLM++III     FT+ ++
Sbjct: 190 PAIVSFAFLRTIRIMKVKR--QTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 249

Query: 258 LGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAELPSPR----TS 317
            G A V++  L LPI+VVI EE  + K K   V +    PI     + +L S        
Sbjct: 250 GGSAAVVIVLLLLPIIVVILEEKKLWKEK--QVALNDPAPINVVTEKPKLDSSEFKDDDG 309

Query: 318 SVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAGGTLTAMDN 377
                 +      SC+  VF PPERG+DYTILQA FSVDMLILF+ TICG GGTLTA+DN
Sbjct: 310 EESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDN 369

Query: 378 LGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTLVLLLSC 437
           LGQIG+SLGY   ++STF SLVSIW + GR  SG  SE    KY F RPL LT+VLLLSC
Sbjct: 370 LGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSC 429

Query: 438 VGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGIASPVGS 497
            GHLLIAF VP  LY ASVIIGFCFGA WPL+FAI+SE+FGLKYY+TLY+   +ASP+GS
Sbjct: 430 AGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGS 489

Query: 498 YIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           Y+ NV+VAGYLYD EA KQ  A+G   V G DL+C G  C+++
Sbjct: 490 YLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKL 528

BLAST of Lsi01G012960 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 548.5 bits (1412), Expect = 7.2e-156
Identity = 286/520 (55.00%), Postives = 360/520 (69.23%), Query Frame = 0

Query: 17  QMTNFAFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDL 76
           +   F      GRWF VFAS LIM+ +GATY+F  YS DIKS+  YDQTTLNL+GFFKDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 77  GSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLLICLGAN 136
           G+NVGV SGLI E+TP W VL IG  MNF GYFMIWL+VT ++ KPK+  MCL IC+GAN
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 137 SQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSKDFILLIAW 196
           SQ F+NTGAL+  V+NFP++RG +LGLLKG+VGLSGAI TQ+Y A YG DSK  ILLIAW
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAW 184

Query: 197 LPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQ 256
           LP AVS + + ++R  E   + + N+L  FY  LYIS+ LA FLM + I + ++ F++  
Sbjct: 185 LPAAVSLVFVYLIR--EKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAA 244

Query: 257 YLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAELPSPRTSSVP 316
           Y   A +  A LF+P+ V +++E  +       ++  S + +       +L   + + V 
Sbjct: 245 YAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVN 304

Query: 317 TTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAGGTLTAMDNLGQ 376
                     SCF  VF PP RGEDYTILQA  S DM+ILFV T CG G +LTA+DNLGQ
Sbjct: 305 GEE---KETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 364

Query: 377 IGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTLVLLLSCVGH 436
           IG SLGY  HT+S+F SLVSIW + GR FSG+ SE+L  KY   RPL +TLVLLLSC GH
Sbjct: 365 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 424

Query: 437 LLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIF 496
           LLIAF VP S+Y AS+++GF FGA  PL+FAI+SELFGLKYY+TL++   +ASP+GSYI 
Sbjct: 425 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 484

Query: 497 NVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           NV+V G LYD+EA KQ+ A GL      DL C G  CY++
Sbjct: 485 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKL 519

BLAST of Lsi01G012960 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 4.9e-88
Identity = 202/529 (38.19%), Postives = 300/529 (56.71%), Query Frame = 0

Query: 24  QVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDLGSNVGVF 83
           ++L  +W  + AS+ I   SGA+Y F +YSA +KS+ +YDQ+TL+ +  FKD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  SGL----------------INEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAM 143
           SGL                I     PWVVL +G +  F GYF+IW SVT  I KP +P M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDS 203
           CL + L A SQTF NT  ++ +V NF    G  +G++KGF+GLSGAIL Q+Y      D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 KDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQ 263
             FILL+A  PT +S L++ +VR+ E +    ++D K+   +  +SL +A +LMI+II++
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETS---VADDKKHLNGLSAVSLIIAAYLMIIIILK 241

Query: 264 NELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAEL 323
           N    +    +     LL  L LP+++        R+ +  G++ T    +P D S   +
Sbjct: 242 NTFGLSSWANIVTLVCLLVMLALPLLIA-------RRAQRDGMEKT----VPHDYSPL-I 301

Query: 324 PSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAGGT 383
            SP+       A    N SS  ++        E+  +LQA   +   +LF+  ICG G  
Sbjct: 302 SSPK-------ATTSGNQSSEGDSKVE-AGLSENLNLLQAMKKLSFWLLFLAMICGMGSG 361

Query: 384 LTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTL 443
           L+ ++N+ QIG SL YS+  I++  SL SIW FLGR  +GYAS+ L  K  + RPL +  
Sbjct: 362 LSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAA 421

Query: 444 VLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGI 503
            L    +GHL+IA G   +LY  SVI+G C+G+ W L+  I SELFG+++  T+++   +
Sbjct: 422 TLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISV 481

Query: 504 ASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           ASP+GSYIF+V++ GY+YD+ A            +G    C G HC+R+
Sbjct: 482 ASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRL 495

BLAST of Lsi01G012960 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 5.6e-84
Identity = 194/519 (37.38%), Postives = 292/519 (56.26%), Query Frame = 0

Query: 29  RWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDLGSNVGVFSGLI- 88
           +W    AS+ I S SGATY FA+YS+ +KSS +YDQ+TL+ +  FKD+G   G+ SG + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 89  -------NEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLLICLGANSQTF 148
                       PWVV+F+G V  F G+F IW SV   I  P +P MCL + L  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 149 SNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDSK--DFILLIAWLP 208
            NT  ++ + RNF Q  G  +G+++GF+GLSGAIL Q+YHA  G +     FILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 209 TAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQNELMFTRIQYL 268
           T V FL +  VRV E   I   +D K+   +  IS+ +A +LM++I ++N L  +R   +
Sbjct: 187 TLVMFLAMPFVRVYETVTI---SDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 246

Query: 269 GCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAELPSPRTSSVPTT 328
               ++L  L  P++V +R   ++R+++                + + L  P    V  T
Sbjct: 247 FSFILVLLLLASPLLVAVR---ALREKR---------------QTLSSLDGP----VLDT 306

Query: 329 AIALANPSSCFENVFRPPER--GEDYTILQATFSVDMLILFVVTICGAGGTLTAMDNLGQ 388
           +  L  PSS   N+F   +    ED  IL+A  +V+  +LF+  +CG G     ++N+ Q
Sbjct: 307 SALLDPPSS---NIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQ 366

Query: 389 IGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTLVLLLSCVGH 448
           IG SL YS+  +++  SL SIW FLGR  +GY S+    K+++ RP+F+ + L +  +GH
Sbjct: 367 IGESLRYSSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGH 426

Query: 449 LLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIF 508
           +++A GV  SLY  SV+IG  +G+ W L+  I SE+FG+++  T+Y    IA P+GSYI 
Sbjct: 427 IIVASGVQGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYIL 485

Query: 509 NVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYR 536
           +VKV GY YD+ A +             D  C G  C+R
Sbjct: 487 SVKVIGYFYDKVASED------------DNSCFGSQCFR 485

BLAST of Lsi01G012960 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 1.6e-83
Identity = 187/529 (35.35%), Postives = 291/529 (55.01%), Query Frame = 0

Query: 22  AFQVLFGRWFTVFASLLIMSVSGATYMFALYSADIKSSFNYDQTTLNLIGFFKDLGSNVG 81
           A ++L  +W  + AS+ I   +G +Y F +YSA +KS+ +YDQ+TL+ +  FKD+G NVG
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 82  VFSGLINEITP--------------PWVVLFIGGVMNFFGYFMIWLSVTHRIPKPKLPAM 141
           V SGL+                   PWVV+ IG ++NF GYF++W SVT  I +P +P M
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 142 CLLICLGANSQTFSNTGALIPSVRNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYGDDS 201
           CL + + A S TF NT  ++ S+ NF    G  +G++KGFVGLSGA+L Q+Y      D 
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 202 KDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILIIIQ 261
           K FILL+A +P+ +S L++ +VRV + + +   ++ K+   +  +SL +A +LMI II++
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYKTSTV---DEKKHLDGLSTLSLIIAAYLMITIILK 241

Query: 262 NELMFTRIQYLGCAFVLLAFLFLPIVVVIREEFSIRKRKLQGVDVTSWLPIPPDPSRAEL 321
           + L            VLL  L  P++V +R      ++ L  V                 
Sbjct: 242 STLSLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSV----------------- 301

Query: 322 PSPRTSSVPTTAIALANPSSCFENVFRPPERGEDYTILQATFSVDMLILFVVTICGAGGT 381
            SP   ++  T                  +  +   +LQA  +VD  +LF+  ICG G  
Sbjct: 302 YSPLVDNLEATT----------SGEILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSG 361

Query: 382 LTAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTL 441
           ++ ++N+ QIG SL Y++  I++  +L +IW F+GR   GY S++L  +  + RPL +  
Sbjct: 362 ISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMAT 421

Query: 442 VLLLSCVGHLLIAFGVPTSLYFASVIIGFCFGALWPLIFAIVSELFGLKYYATLYSISGI 501
            L    +GHL+IA G   +LY  S+I+G C+G+ W L+  I SELFG+K+  T+Y+   I
Sbjct: 422 TLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISI 481

Query: 502 ASPVGSYIFNVKVAGYLYDQEARKQMAAIGLRSVAGGDLDCKGVHCYRV 537
           ASP+GSYIF+V++ GY+YD            R++ G    C G HC+R+
Sbjct: 482 ASPMGSYIFSVRLIGYIYD------------RTIIGEGNTCYGPHCFRL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3CH391.8e-27191.17uncharacterized protein LOC103500834 OS=Cucumis melo OX=3656 GN=LOC103500834 PE=... [more]
A0A5D3C8493.1e-27190.98Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7V9Y51.2e-27090.98Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1CR801.7e-25084.89protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1ECZ33.3e-24985.45protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_038882767.11.5e-27592.16protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_008462497.13.7e-27191.17PREDICTED: uncharacterized protein LOC103500834 [Cucumis melo][more]
TYK07328.16.3e-27190.98protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
XP_004143363.28.3e-27191.53uncharacterized protein LOC101203981 [Cucumis sativus] >KAE8647411.1 hypothetica... [more]
KAA0063797.12.4e-27090.98protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G39210.13.6e-17158.89Major facilitator superfamily protein [more]
AT2G28120.17.2e-15655.00Major facilitator superfamily protein [more]
AT1G74780.14.9e-8838.19Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.15.6e-8437.38Major facilitator superfamily protein [more]
AT1G18940.11.6e-8335.35Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..276
e-value: 2.0E-87
score: 292.8
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 328..522
e-value: 1.7E-11
score: 45.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 30..507
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 11..537
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 11..537
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 28..536
e-value: 4.31951E-95
score: 296.47

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G012960.1Lsi01G012960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity