Lsi01G012240 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G012240 vs. ExPASy Swiss-Prot
Match: F4HW17 (Transmembrane 9 superfamily member 5 OS=Arabidopsis thaliana OX=3702 GN=TMN5 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.1e-39
Identity = 157/561 (27.99%), Postives = 244/561 (43.49%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y Y+ LPFC  G  + K+ +L E+L GD L ++ Y+LKF   ++   LC K +T  D+
Sbjct: 50  ETYQYYDLPFCRRGPVIEKQETLGEVLNGDRLMSSLYKLKFREDKTHFVLCRKRLTSSDI 109

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQ-----KFYLFNHIEFNVDFMENQ 120
            RF+  IA  + +QMY+D +     VG+V E    GQ     K+Y+F+H++FNV +  ++
Sbjct: 110 ARFRDIIAQDYYFQMYYDDLPLWGFVGKV-EGDYFGQGEKHTKYYIFSHLKFNVLYNADK 169

Query: 121 VIGISVVNSLDSSVDITNFTETLVEFSYSVFWN---EIKPIHNSSY----FIPVNKEASW 180
           VI I+  +     VDI+  TE  V+F+YSV WN   E      + Y    F P++++  +
Sbjct: 170 VIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLTSERSETRMNKYSRASFHPISQKIHF 229

Query: 181 VLEENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRN------------RQPHGN 240
                   FL+S+          V++ L+    +L    L+N            R+  G 
Sbjct: 230 F------SFLNSI---------TVVVLLIGLISFLFMRHLKNELRSYSIGDEEERKEAGW 289

Query: 241 VLRLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDG-IYPCDHVKISVDLILAY 300
            L ++  V  CP   S L A LG GTQ LI+I+  FA A+ G +YP +   +   L++ Y
Sbjct: 290 KL-VHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPYNRGMLLTSLVIMY 349

Query: 301 CITSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIY 360
            +TS+++ + + +FH +F     K  V   G LY  P FI + +     IT        Y
Sbjct: 350 TLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNTVAIT--------Y 409

Query: 361 NLLVA-GFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRSPSTL----------- 420
               A  FG+ ++I L    +   +           R  LL  + PS +           
Sbjct: 410 GATAALPFGTIVIIILIFTLLNIPFL--MLGGVLGNRFGLLEFQPPSAVKRNPREIPPQN 469

Query: 421 WYMKTPAQMILWGLGIFLPISPLMDEIYASLWGLKV------------------------ 478
           WY +   Q+ L G   F  +     ++YASLWG K+                        
Sbjct: 470 WYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGII 529

BLAST of Lsi01G012240 vs. ExPASy Swiss-Prot
Match: Q9FHT4 (Transmembrane 9 superfamily member 4 OS=Arabidopsis thaliana OX=3702 GN=TMN4 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.4e-36
Identity = 141/550 (25.64%), Postives = 237/550 (43.09%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC       K+ +L E+L GD L +  Y+L+F   ++    C K ++ +D+
Sbjct: 53  ETYRYFDLPFCSSAPVKEKKEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDV 112

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIE--KTGLGQ-KFYLFNHIEFNVDFMENQVI 120
            +F+  IA  + +QMY+D +     +G+V++  KT   + K+YLFNH++F + + +++VI
Sbjct: 113 AKFRDVIAKDYYFQMYYDDLPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVI 172

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIK-------PIHNSSYFIPVNKEASWVL 180
            I V    +  VD+T   E  V+F+Y+V W E +         ++ +  +P + E  W  
Sbjct: 173 EIIVRTDQNFLVDLTEDKEVQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWF- 232

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   +  + +             HG+V 
Sbjct: 233 ----SIINSCVTVLLLTGFLATILMRVLKNDFV--KYAHDEEAVDDQEETGWKLIHGDVF 292

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SLL A LG GTQ   + V  F  A  G+ YP +   +   L++ Y +
Sbjct: 293 RF-------PKHKSLLAAALGSGTQLFTLAVFIFMLALVGVFYPYNRGALFTALVVIYAL 352

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + A +F+ +         V  TG+L+  P+ I         I         +  
Sbjct: 353 TSGIAGYTAASFYCQLEGTNWVRNVILTGSLFCGPLLITFSFLNTVAIAYQATAALPFGT 412

Query: 361 LVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRS-PSTLWYMKTPAQMILWG 420
           +V  F    L+    + +  +     K+      R   Y R  P   WY +T  QM + G
Sbjct: 413 IVVIFLIWALVTSPLLILGGIAGKNRKSEFQAPCRTTKYPREIPPMRWYRRTLPQMAMAG 472

Query: 421 LGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT-----------ITESD 478
              FLP S +  E+Y   AS+WG ++   +  L   FL++++ T           +   D
Sbjct: 473 ---FLPFSAIYIELYYIFASVWGHRIYTIYSILSIVFLILVIVTAFITVALTYFQLAAED 532

BLAST of Lsi01G012240 vs. ExPASy Swiss-Prot
Match: Q940S0 (Transmembrane 9 superfamily member 2 OS=Arabidopsis thaliana OX=3702 GN=TMN2 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-33
Identity = 139/550 (25.27%), Postives = 231/550 (42.00%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC P     K+ +L E+L GD L +  Y+L F   +     C K ++++++
Sbjct: 52  ETYRYFDLPFCIPEGVKEKKEALGEVLNGDRLVSAPYKLNFRDEKESEVYCNKKLSKEEV 111

Query: 61  NRFKSAIANKFEYQMYFD--KIW-FGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVI 120
            +F+ A+   + +QMY+D   IW F  KV + I+      K++L+ HI+F + + +++VI
Sbjct: 112 KQFRKAVEKDYYFQMYYDDLPIWGFIGKVDKDIKSDPSEFKYFLYKHIQFEILYNKDRVI 171

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIK-------PIHNSSYFIPVNKEASWVL 180
            IS      S VD+T   E   EF Y+V W E +         ++ S  +P + E  W  
Sbjct: 172 EISARMDPHSLVDLTEDKEVDAEFMYTVKWKETETPFEKRMEKYSMSSSLPHHLEIHWF- 231

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   + ++ +             HG+V 
Sbjct: 232 ----SIINSCVTVLLLTGFLATILMRVLKNDFM--KYAQDEEAADDQEETGWKYIHGDVF 291

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SL  A+LG GTQ   + +  F  A  G+ YP +   +   L++ Y +
Sbjct: 292 RF-------PTHNSLFAASLGSGTQLFTLTIFIFMLALVGVFYPYNRGALFTALVVIYAL 351

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + + +F+ +         +  TG L+  P+F+         IT        +  
Sbjct: 352 TSGIAGYTSASFYCQLEGKSWVRNLLLTGCLFCGPLFLTFCFLNTVAITYTATAALPFGT 411

Query: 361 LVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRS-PSTLWYMKTPAQMILWG 420
           +V       L+    + +  +     K       R   Y R  P   WY     QM + G
Sbjct: 412 IVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPWYRSAIPQMAMAG 471

Query: 421 LGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT--ITESDREFVLP--- 478
              FLP S +  E+Y   AS+WG ++   +  LF  F+++I+ T  IT +   F L    
Sbjct: 472 ---FLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILIIVTAFITVALTYFQLAAED 531

BLAST of Lsi01G012240 vs. ExPASy Swiss-Prot
Match: Q9ZPS7 (Transmembrane 9 superfamily member 3 OS=Arabidopsis thaliana OX=3702 GN=TMN3 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-31
Identity = 137/558 (24.55%), Postives = 229/558 (41.04%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC P     K+ +L E+L GD L +  Y+L F   +     C+K ++ +++
Sbjct: 52  ETYRYFDLPFCIPEGVKDKKEALGEVLNGDRLVSAPYKLNFRDEKDSEVYCKKKLSREEV 111

Query: 61  NRFKSAIANKFEYQMYFD--KIW-FGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVI 120
             F+ A+   + +QMY+D   IW F  KV +  +      K++L+ HI+F + + +++VI
Sbjct: 112 EHFRRAVEKDYYFQMYYDDLPIWGFIGKVDKESKSDPSEFKYFLYKHIQFEILYNKDRVI 171

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPI-------HNSSYFIPVNKEASWVL 180
            I+      S VD+T   E   EF Y+V W E +         +  S  +P + E  W  
Sbjct: 172 EINARMDPHSLVDLTEDKEVDAEFMYTVKWKETETSFEKRMDKYAMSSSLPHHLEIHWF- 231

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   + ++ +             HG+V 
Sbjct: 232 ----SIINSCVTVLLLTGFLATILMRVLKNDFM--KYAQDEEAADDQEETGWKYIHGDVF 291

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SL  A+LG GTQ   + +  F  +  G+ YP +   +   L++ Y +
Sbjct: 292 RF-------PKNKSLFAASLGSGTQLFTLTIFIFMLSLVGVFYPYNRGALFTALVVIYAL 351

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + A +F+ +         +  TG L+  P+F+         I         Y+ 
Sbjct: 352 TSGIAGYTASSFYCQLEGKNWVRNLLLTGGLFCGPLFLTFCFLNTVAIA--------YSA 411

Query: 361 LVA-GFGSAILIYLCCIAIRD--------LYRPEHKTATCPTRRLLLYNRSPSTLWYMKT 420
             A  FG+ I+I L    +            +        P R        P   WY   
Sbjct: 412 TAALPFGTIIVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPVRTTKYPREIPPLPWYRSA 471

Query: 421 PAQMILWGLGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT-------- 478
             QM + G   FLP S +  E+Y   AS+WG ++   +  LF  F+++++ T        
Sbjct: 472 VPQMAMAG---FLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITVALT 531

BLAST of Lsi01G012240 vs. ExPASy Swiss-Prot
Match: Q54ZW0 (Putative phagocytic receptor 1b OS=Dictyostelium discoideum OX=44689 GN=phg1b PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.7e-26
Identity = 135/559 (24.15%), Postives = 226/559 (40.43%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y +++LPFC P     K+  L EIL GD    + Y+  F  S     LCE  + ++D+
Sbjct: 50  ETYEFYTLPFCKPSSISYKKTKLGEILQGDSAVLSDYQFPFKSSFENKQLCEYTLKKEDI 109

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
            +FK AI   +  +M +D +   S VG V +      ++YL+NHI F  D+  +QVI ++
Sbjct: 110 EKFKKAIGEYYYAEMIYDDLPIFSFVGTVDDSDLTNIRYYLYNHIPFEFDYNGDQVIRVN 169

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNKEASWVLEENRRLFLSS 180
           +       +++++  E  ++ +YS  W             P   E S  ++     F   
Sbjct: 170 IDTEHIKVIELSDQDEITLKLTYSAKWQ------------PTEHEFSKRMDLYEEFFTKE 229

Query: 181 L---WLWSIMAFWWVILPLVVASPYLVPYFLRN----------------------RQPHG 240
           L   WL S+M  +++++ L      ++   L+N                      +  HG
Sbjct: 230 LEIHWL-SVMNSFFLVVLLTAFLAIMIMKILKNDYSRYSKTDEEEDSDYQEDYGWKLVHG 289

Query: 241 NVLRLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILA 300
           +V R        P + ++  A  G+G Q + I+    A +  G+ YP +   +    I+ 
Sbjct: 290 DVFRF-------PPYKNVFSAFYGIGWQFISIVCGILALSLFGMFYPNNGGNMYTAGIVL 349

Query: 301 YCITSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGIT------TP 360
           Y +TS +S + +   ++          +  T  L++ P+FI V+L     IT       P
Sbjct: 350 YALTSGISGYQSAKMYKNMGGNKWAWNIVLTATLFVAPLFIVVILSNTVAITWHSTVALP 409

Query: 361 MVD--NAIYNLLVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRSPSTLWYM 420
           ++     I   L  GF    L  +  IA R L   E+  A C T+        P   WY 
Sbjct: 410 ILTMIEVITIWLFVGFP---LTVVGGIAGRRL--SENFEAPCRTKN--FPREVPPIQWYR 469

Query: 421 KTPAQMILWGLGIFLPISPLMDE---IYASLWGLKVCGSFLTLFAAFLMVILPT--ITES 477
           + P Q+++ G   FLP S +  E   I+ S+WG      +  L   FL++I  T  IT +
Sbjct: 470 RLPCQILIAG---FLPFSAIYIELFYIFNSVWGHSTYTLYGILCLVFLILINVTVCITVA 529

BLAST of Lsi01G012240 vs. ExPASy TrEMBL
Match: A0A5D3CAR6 (Transmembrane 9 superfamily member OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001350 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.7e-213
Identity = 384/538 (71.38%), Postives = 429/538 (79.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           DA+ YFSLPFCPPGE VSKRRSLNEILAGDCL NTQYELKFGVSE E FLCEKY+TEDDL
Sbjct: 71  DAFPYFSLPFCPPGEKVSKRRSLNEILAGDCLMNTQYELKFGVSEPEVFLCEKYLTEDDL 130

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
             FK AIAN+F YQMYFD IWF SKVGEVIE  GLGQK YLFN IEFNVDFME++V+ IS
Sbjct: 131 RIFKFAIANEFVYQMYFDNIWFESKVGEVIEIPGLGQKLYLFNRIEFNVDFMEDKVLSIS 190

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNKE-ASWVLEENRRLFLS 180
           VVNSLDSS DIT  T+ LVEFSYSVFWNEIKPI NSSYFIP ++E ASWVLE+NRRLF S
Sbjct: 191 VVNSLDSSADITILTDPLVEFSYSVFWNEIKPIDNSSYFIPGDREKASWVLEDNRRLFWS 250

Query: 181 SLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLGATLG 240
           SLWLWSI+AFWW+ILPLVVA+PYL  YFL+NRQPHGN+ R N K C CP +TSLLGA LG
Sbjct: 251 SLWLWSILAFWWIILPLVVAAPYLFKYFLKNRQPHGNIHRFNGKACSCPKYTSLLGAILG 310

Query: 241 VGTQHL-IIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPIGS 300
           VGTQHL +II +   + YDGIYPC+H +IS+DL+L YCITSV+SA +AR+FHE+FSPIGS
Sbjct: 311 VGTQHLMLIIAMLLVSEYDGIYPCNHERISIDLVLMYCITSVVSASIARSFHEKFSPIGS 370

Query: 301 KECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAIRDL 360
           KECVFQTGALY FPVFIAV+LGKIFGI+TP+VD+ I  LLVAGFGSAI+IY+CCIA R++
Sbjct: 371 KECVFQTGALYFFPVFIAVILGKIFGISTPIVDSVICYLLVAGFGSAIMIYICCIAPRNI 430

Query: 361 YRPEHKTATCPTRRLLLYNRS---PSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           YRPE   ATC TR+LLLYNRS     TLWYMKTPAQM+L GLGIFLPISPLMD+IYASLW
Sbjct: 431 YRPERNAATCHTRKLLLYNRSSPPAPTLWYMKTPAQMMLEGLGIFLPISPLMDDIYASLW 490

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLK+C SFLTLFAAFLMV+L T                                      
Sbjct: 491 GLKICSSFLTLFAAFLMVVLTTFISGMALTSVQLLKNDYNWWWRSILRGGSPAIYMFGYG 550

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGY-TKKRS 488
                   +E+DR FVLPLVYNCCICYSFFLV GTVGFGASL+AFK+YM+G  TKKRS
Sbjct: 551 IYFISKIRSENDRGFVLPLVYNCCICYSFFLVFGTVGFGASLVAFKFYMMGCDTKKRS 608

BLAST of Lsi01G012240 vs. ExPASy TrEMBL
Match: A0A1S3CH77 (Transmembrane 9 superfamily member OS=Cucumis melo OX=3656 GN=LOC103500897 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.7e-213
Identity = 384/538 (71.38%), Postives = 429/538 (79.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           DA+ YFSLPFCPPGE VSKRRSLNEILAGDCL NTQYELKFGVSE E FLCEKY+TEDDL
Sbjct: 71  DAFPYFSLPFCPPGEKVSKRRSLNEILAGDCLMNTQYELKFGVSEPEVFLCEKYLTEDDL 130

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
             FK AIAN+F YQMYFD IWF SKVGEVIE  GLGQK YLFN IEFNVDFME++V+ IS
Sbjct: 131 RIFKFAIANEFVYQMYFDNIWFESKVGEVIEIPGLGQKLYLFNRIEFNVDFMEDKVLSIS 190

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNKE-ASWVLEENRRLFLS 180
           VVNSLDSS DIT  T+ LVEFSYSVFWNEIKPI NSSYFIP ++E ASWVLE+NRRLF S
Sbjct: 191 VVNSLDSSADITILTDPLVEFSYSVFWNEIKPIDNSSYFIPGDREKASWVLEDNRRLFWS 250

Query: 181 SLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLGATLG 240
           SLWLWSI+AFWW+ILPLVVA+PYL  YFL+NRQPHGN+ R N K C CP +TSLLGA LG
Sbjct: 251 SLWLWSILAFWWIILPLVVAAPYLFKYFLKNRQPHGNIHRFNGKACSCPKYTSLLGAILG 310

Query: 241 VGTQHL-IIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPIGS 300
           VGTQHL +II +   + YDGIYPC+H +IS+DL+L YCITSV+SA +AR+FHE+FSPIGS
Sbjct: 311 VGTQHLMLIIAMLLVSEYDGIYPCNHERISIDLVLMYCITSVVSASIARSFHEKFSPIGS 370

Query: 301 KECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAIRDL 360
           KECVFQTGALY FPVFIAV+LGKIFGI+TP+VD+ I  LLVAGFGSAI+IY+CCIA R++
Sbjct: 371 KECVFQTGALYFFPVFIAVILGKIFGISTPIVDSVICYLLVAGFGSAIMIYICCIAPRNI 430

Query: 361 YRPEHKTATCPTRRLLLYNRS---PSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           YRPE   ATC TR+LLLYNRS     TLWYMKTPAQM+L GLGIFLPISPLMD+IYASLW
Sbjct: 431 YRPERNAATCHTRKLLLYNRSSPPAPTLWYMKTPAQMMLEGLGIFLPISPLMDDIYASLW 490

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLK+C SFLTLFAAFLMV+L T                                      
Sbjct: 491 GLKICSSFLTLFAAFLMVVLTTFISGMALTSVQLLKNDYNWWWRSILRGGSPAIYMFGYG 550

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGY-TKKRS 488
                   +E+DR FVLPLVYNCCICYSFFLV GTVGFGASL+AFK+YM+G  TKKRS
Sbjct: 551 IYFISKIRSENDRGFVLPLVYNCCICYSFFLVFGTVGFGASLVAFKFYMMGCDTKKRS 608

BLAST of Lsi01G012240 vs. ExPASy TrEMBL
Match: A0A0A0KKH6 (Transmembrane 9 superfamily member OS=Cucumis sativus OX=3659 GN=Csa_6G452130 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 5.2e-199
Identity = 359/503 (71.37%), Postives = 403/503 (80.12%), Query Frame = 0

Query: 34  NTQYELKFGVSESEGFLCEKYMTEDDLNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKT 93
           NTQYELKFGVS++EGFLCEKYMTEDDL  FK AI N F YQMYFD IWFGSKVGEVIE+ 
Sbjct: 2   NTQYELKFGVSDTEGFLCEKYMTEDDLRIFKFAIENNFVYQMYFDNIWFGSKVGEVIEEP 61

Query: 94  GLGQKFYLFNHIEFNVDFMENQVIGISVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPI 153
           G GQKFYLFNHIEFNVDFME QV+ ISVVNSLDSSVDIT  TE LVEFSYSVFWNEIKPI
Sbjct: 62  GFGQKFYLFNHIEFNVDFMEGQVLDISVVNSLDSSVDITILTEPLVEFSYSVFWNEIKPI 121

Query: 154 HNSSYFIPVNKE-ASWVLEENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQ 213
            NSSYFIP  +E ASWVLE+NRRLF SSLWLWSI+AFWW+ LP+VVASPYL  YFL+NRQ
Sbjct: 122 DNSSYFIPGEREKASWVLEDNRRLFWSSLWLWSILAFWWIFLPVVVASPYLFKYFLKNRQ 181

Query: 214 PHGNVLRLNDKVCFCPMFTSLLGATLGVGTQHLI-IIVLFFATAYDGIYPCDHVKISVDL 273
           PHGN+ R N K CFCPM+TSLLGA LGVGTQHLI IIV+ F + YDGI+PC+H +ISVDL
Sbjct: 182 PHGNIHRFNAKACFCPMYTSLLGAILGVGTQHLILIIVMLFVSEYDGIFPCNHERISVDL 241

Query: 274 ILAYCITSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVD 333
           +L YC+TSV+SA +AR+FHE FSPIGSKECV QTGALY FPVF+AV+LGKIFGI+TPMVD
Sbjct: 242 VLLYCVTSVVSASIARSFHERFSPIGSKECVLQTGALYFFPVFMAVILGKIFGISTPMVD 301

Query: 334 NAIYNLLVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNR-SPSTLWYMKTPA 393
           + I  LL AGFGSAI+IY+CCIA R++YRPE   ATC TRRL+++NR SP TLWYMK+PA
Sbjct: 302 SVINYLLAAGFGSAIMIYICCIAPRNIYRPERNVATCHTRRLVIHNRSSPPTLWYMKSPA 361

Query: 394 QMILWGLGIFLPISPLMDEIYASLWGLKVCGSFLTLFAAFLMVILPTI------------ 453
           QMIL GLGIFLPIS LMD+I+ASLWGLK+CGSFLTLF+AFLMV+L T             
Sbjct: 362 QMILEGLGIFLPISLLMDDIFASLWGLKICGSFLTLFSAFLMVVLSTFVSGIALTSIQLL 421

Query: 454 ---------------------------------TESDREFVLPLVYNCCICYSFFLVLGT 488
                                            +ESDR FVLPLVYNCCICYSFFLVLGT
Sbjct: 422 KDDYNWWWRSVLRGGSPAVYMFGYGIYFLSKIRSESDRGFVLPLVYNCCICYSFFLVLGT 481

BLAST of Lsi01G012240 vs. ExPASy TrEMBL
Match: A0A6J1HF92 (Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111463439 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 6.4e-189
Identity = 346/537 (64.43%), Postives = 396/537 (73.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKR-RSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDD 60
           DA SYFSLPFCPPGE + KR +SLNEILAGDCLTNTQY+L+FGV  SEGFLC+KYMTEDD
Sbjct: 65  DADSYFSLPFCPPGEKLHKRKKSLNEILAGDCLTNTQYDLRFGVPVSEGFLCDKYMTEDD 124

Query: 61  LNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGI 120
           L RFK+AI NKFEYQMYF  IWFGSKVGE IE+TGLG+K+YLFNHIEFN+DFME+QV G+
Sbjct: 125 LKRFKTAIENKFEYQMYFADIWFGSKVGEAIEETGLGKKYYLFNHIEFNLDFMEDQVKGV 184

Query: 121 SVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNK----EASWVLEENRR 180
           ++VNSLD SV ITN TET VEFSYSV WNEIKP +NSSYF   NK    +ASWVLEENR 
Sbjct: 185 NIVNSLDFSVAITNITETPVEFSYSVVWNEIKPTNNSSYFKSWNKAPYGKASWVLEENRG 244

Query: 181 LFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLG 240
           LF S +WLW+ ++FWWV LPLVVASPYL  Y   NRQPH    R NDKVC CPMFTSLLG
Sbjct: 245 LFWSGIWLWTTISFWWVALPLVVASPYLFRYVTLNRQPHQG-SRFNDKVCQCPMFTSLLG 304

Query: 241 ATLGVGTQHLIIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSP 300
           A LGVGTQHLIIIVL F +AY GIYPC+  +ISVD++LAYC+TSV SAF+ R+FHE+FSP
Sbjct: 305 ALLGVGTQHLIIIVLLFVSAYQGIYPCNRERISVDIVLAYCLTSVPSAFIGRSFHEKFSP 364

Query: 301 IGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAI 360
           +  KECVF TGALY +P F+AVLL KIF   + MV++AIY L +AG GSAIL+YLCCIA 
Sbjct: 365 LKLKECVFLTGALYFYPAFLAVLLAKIFVSNSLMVNDAIYILSIAGIGSAILVYLCCIAT 424

Query: 361 RDLYRPEHKTATCPTRRLLLYNRSPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           R  Y PEH    C TR + ++N   S+LWYMKTPAQM+L GL +FLP+  +MD+IYASLW
Sbjct: 425 RKWYEPEHDVTACATRMVDVFNPPASSLWYMKTPAQMVLVGLSVFLPVCLVMDDIYASLW 484

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLKVCGSF TLFA F  VI+ TI                                     
Sbjct: 485 GLKVCGSFFTLFAVFSTVIITTIISAYALTGVQLLKNDYNWWWRSVLRGGSPAILMFVYG 544

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGYTKKRS 488
                   TESDREF+  LVYNCCICYS FLVLGTV F AS  AF++YM    KKRS
Sbjct: 545 IYFLSKIKTESDREFLPLLVYNCCICYSLFLVLGTVSFRASHFAFRFYMAVVAKKRS 600

BLAST of Lsi01G012240 vs. ExPASy TrEMBL
Match: A0A6J1IAJ2 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111471163 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.4e-188
Identity = 346/537 (64.43%), Postives = 396/537 (73.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKR-RSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDD 60
           DA SYFSLPFCPPGE + KR +SLNEILAGDCLTNTQYEL+FGV  SEGFLC+KYMTEDD
Sbjct: 65  DADSYFSLPFCPPGEKLHKRKKSLNEILAGDCLTNTQYELRFGVPVSEGFLCDKYMTEDD 124

Query: 61  LNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGI 120
           L RFK+AI NKFEYQMYF  IWFGSKVGE IE+TGLG+K+YLFNHIEFN+DFME+QV G+
Sbjct: 125 LKRFKTAIENKFEYQMYFADIWFGSKVGEAIEETGLGKKYYLFNHIEFNLDFMEDQVKGV 184

Query: 121 SVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNK----EASWVLEENRR 180
           ++VNSLDSSV ITN TET VEFSYSV WNEIKP +NS+YF    K    +ASWVLEENR 
Sbjct: 185 NIVNSLDSSVAITNITETPVEFSYSVVWNEIKPTNNSNYFKSRYKAPYGKASWVLEENRG 244

Query: 181 LFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLG 240
           LF S +WLW+ ++FWWV LPLVVASPYL  Y + NRQPH    R NDKVC CPMFTSLLG
Sbjct: 245 LFWSGIWLWTTISFWWVALPLVVASPYLFRYVMSNRQPHQG-SRFNDKVCQCPMFTSLLG 304

Query: 241 ATLGVGTQHLIIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSP 300
           A LGVGTQHLII+VL F +AY GIYPC+  +ISVD++LAYC+TSV SAF+ R+FHE+FSP
Sbjct: 305 ALLGVGTQHLIIMVLLFVSAYQGIYPCNRERISVDIVLAYCLTSVPSAFIGRSFHEKFSP 364

Query: 301 IGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAI 360
           +  KECVF TGALY +P FIAVLL KIF   + MV++AIY L +AG GSAIL+YLCCIA 
Sbjct: 365 LKLKECVFLTGALYFYPAFIAVLLAKIFVSNSLMVNDAIYILSIAGIGSAILVYLCCIAT 424

Query: 361 RDLYRPEHKTATCPTRRLLLYNRSPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           R  Y PEH    C TR + ++N   S+LWYMKTPAQM+L GL IFLPI  +MD+IYASLW
Sbjct: 425 RKWYEPEHDVTACATRMVDVFNPPASSLWYMKTPAQMVLVGLSIFLPICLVMDDIYASLW 484

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLK CGSF TLF  F MVI+ TI                                     
Sbjct: 485 GLKACGSFFTLFTVFSMVIITTIISAYALTGVQLLKNDYNWWWRSVLRGGSPAILMFVYG 544

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGYTKKRS 488
                   TESDREF+  LVYNCCICYS FLVLG+V F AS  AF++YM    KKRS
Sbjct: 545 IYFLSKIKTESDREFLPLLVYNCCICYSLFLVLGSVSFRASHFAFRFYMAVVAKKRS 600

BLAST of Lsi01G012240 vs. NCBI nr
Match: XP_038880907.1 (transmembrane 9 superfamily member 5-like [Benincasa hispida])

HSP 1 Score: 854.7 bits (2207), Expect = 3.8e-244
Identity = 429/536 (80.04%), Postives = 454/536 (84.70%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           DAYSYFSLPFCPPGEG+SKRR+LNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTE DL
Sbjct: 64  DAYSYFSLPFCPPGEGISKRRTLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEHDL 123

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
             F+ +IANK EYQMYFD IWFGSKVGEVIE TGLGQKFYLFNHIEFNVDFMENQV+GIS
Sbjct: 124 KMFRFSIANKLEYQMYFDDIWFGSKVGEVIETTGLGQKFYLFNHIEFNVDFMENQVMGIS 183

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNK----EASWVLEENRRL 180
           VVNSLDSSVDITN TETLVEFSYSVFWNEIK I NSSYFIP N+    +A WVLEENRRL
Sbjct: 184 VVNSLDSSVDITNCTETLVEFSYSVFWNEIKSIENSSYFIPGNRASAEKAMWVLEENRRL 243

Query: 181 FLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLGA 240
           F SSLWLWSI+AFWW+ILPLV+ASPYL  YFLRNRQPHG + RLNDK+CFCPMFTSLLGA
Sbjct: 244 FWSSLWLWSILAFWWIILPLVLASPYLFRYFLRNRQPHGTIRRLNDKMCFCPMFTSLLGA 303

Query: 241 TLGVGTQHLIIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPI 300
            LGVGTQHLIIIVL F +AYDGIYPCDH KISVDL+LAYCITSVLSAFMAR+FHE+FSPI
Sbjct: 304 ILGVGTQHLIIIVLLFVSAYDGIYPCDHEKISVDLVLAYCITSVLSAFMARSFHEKFSPI 363

Query: 301 GSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAIR 360
           GSKECVFQTGALY FPVFIAV+LG +FGI+TPMVDNAIYNLL+AGFGSAIL+Y+ CIAIR
Sbjct: 364 GSKECVFQTGALYFFPVFIAVVLGMVFGISTPMVDNAIYNLLLAGFGSAILMYMWCIAIR 423

Query: 361 DLYRPEHKTATCPTRRLLLYNRSPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLWG 420
           D YRPE  TATC TRRLLLYNRSPS LWYMKTPAQM+L GLGIFLPISPLMD+IYASLWG
Sbjct: 424 DFYRPERNTATCSTRRLLLYNRSPSPLWYMKTPAQMVLGGLGIFLPISPLMDDIYASLWG 483

Query: 421 LKVCGSFLTLFAAFLMVILPTI-------------------------------------- 480
           LKVCGSFLTLFAAFLMVIL TI                                      
Sbjct: 484 LKVCGSFLTLFAAFLMVILTTIVSGVTLTSIQLLKHDYDWWWRSVLRGGSPALYMFGYGI 543

Query: 481 -------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGYTKKRS 488
                  TESD+EFVLPLVYNCCICYSFFLVLGTVGFGASL AFKYYM GYTKKRS
Sbjct: 544 YFLSKIKTESDKEFVLPLVYNCCICYSFFLVLGTVGFGASLFAFKYYMAGYTKKRS 599

BLAST of Lsi01G012240 vs. NCBI nr
Match: XP_031743866.1 (transmembrane 9 superfamily member 5-like [Cucumis sativus])

HSP 1 Score: 763.5 bits (1970), Expect = 1.1e-216
Identity = 387/536 (72.20%), Postives = 432/536 (80.60%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           DA+ YFSLPFCPPGE VSKRR LNEILAGDCL NTQYELKFGVS++EGFLCEKYMTEDDL
Sbjct: 65  DAFPYFSLPFCPPGEKVSKRRYLNEILAGDCLMNTQYELKFGVSDTEGFLCEKYMTEDDL 124

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
             FK AI N F YQMYFD IWFGSKVGEVIE+ G GQKFYLFNHIEFNVDFME QV+ IS
Sbjct: 125 RIFKFAIENNFVYQMYFDNIWFGSKVGEVIEEPGFGQKFYLFNHIEFNVDFMEGQVLDIS 184

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNKE-ASWVLEENRRLFLS 180
           VVNSLDSSVDIT  TE LVEFSYSVFWNEIKPI NSSYFIP  +E ASWVLE+NRRLF S
Sbjct: 185 VVNSLDSSVDITILTEPLVEFSYSVFWNEIKPIDNSSYFIPGEREKASWVLEDNRRLFWS 244

Query: 181 SLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLGATLG 240
           SLWLWSI+AFWW+ LP+VVASPYL  YFL+NRQPHGN+ R N K CFCPM+TSLLGA LG
Sbjct: 245 SLWLWSILAFWWIFLPVVVASPYLFKYFLKNRQPHGNIHRFNAKACFCPMYTSLLGAILG 304

Query: 241 VGTQHLI-IIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPIGS 300
           VGTQHLI IIV+ F + YDGI+PC+H +ISVDL+L YC+TSV+SA +AR+FHE FSPIGS
Sbjct: 305 VGTQHLILIIVMLFVSEYDGIFPCNHERISVDLVLLYCVTSVVSASIARSFHERFSPIGS 364

Query: 301 KECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAIRDL 360
           KECV QTGALY FPVF+AV+LGKIFGI+TPMVD+ I  LL AGFGSAI+IY+CCIA R++
Sbjct: 365 KECVLQTGALYFFPVFMAVILGKIFGISTPMVDSVINYLLAAGFGSAIMIYICCIAPRNI 424

Query: 361 YRPEHKTATCPTRRLLLYNR-SPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLWGL 420
           YRPE   ATC TRRL+++NR SP TLWYMK+PAQMIL GLGIFLPIS LMD+I+ASLWGL
Sbjct: 425 YRPERNVATCHTRRLVIHNRSSPPTLWYMKSPAQMILEGLGIFLPISLLMDDIFASLWGL 484

Query: 421 KVCGSFLTLFAAFLMVILPTI--------------------------------------- 480
           K+CGSFLTLF+AFLMV+L T                                        
Sbjct: 485 KICGSFLTLFSAFLMVVLSTFVSGIALTSIQLLKDDYNWWWRSVLRGGSPAVYMFGYGIY 544

Query: 481 ------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIG-YTKKRS 488
                 +ESDR FVLPLVYNCCICYSFFLVLGTVGFGASL+AFK+YM+G  +KKRS
Sbjct: 545 FLSKIRSESDRGFVLPLVYNCCICYSFFLVLGTVGFGASLVAFKFYMMGCNSKKRS 600

BLAST of Lsi01G012240 vs. NCBI nr
Match: XP_008462569.1 (PREDICTED: transmembrane 9 superfamily member 5-like isoform X1 [Cucumis melo] >KAA0025258.1 transmembrane 9 superfamily member 5-like isoform X1 [Cucumis melo var. makuwa] >TYK07409.1 transmembrane 9 superfamily member 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 751.9 bits (1940), Expect = 3.4e-213
Identity = 384/538 (71.38%), Postives = 429/538 (79.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           DA+ YFSLPFCPPGE VSKRRSLNEILAGDCL NTQYELKFGVSE E FLCEKY+TEDDL
Sbjct: 71  DAFPYFSLPFCPPGEKVSKRRSLNEILAGDCLMNTQYELKFGVSEPEVFLCEKYLTEDDL 130

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGIS 120
             FK AIAN+F YQMYFD IWF SKVGEVIE  GLGQK YLFN IEFNVDFME++V+ IS
Sbjct: 131 RIFKFAIANEFVYQMYFDNIWFESKVGEVIEIPGLGQKLYLFNRIEFNVDFMEDKVLSIS 190

Query: 121 VVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNKE-ASWVLEENRRLFLS 180
           VVNSLDSS DIT  T+ LVEFSYSVFWNEIKPI NSSYFIP ++E ASWVLE+NRRLF S
Sbjct: 191 VVNSLDSSADITILTDPLVEFSYSVFWNEIKPIDNSSYFIPGDREKASWVLEDNRRLFWS 250

Query: 181 SLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLGATLG 240
           SLWLWSI+AFWW+ILPLVVA+PYL  YFL+NRQPHGN+ R N K C CP +TSLLGA LG
Sbjct: 251 SLWLWSILAFWWIILPLVVAAPYLFKYFLKNRQPHGNIHRFNGKACSCPKYTSLLGAILG 310

Query: 241 VGTQHL-IIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPIGS 300
           VGTQHL +II +   + YDGIYPC+H +IS+DL+L YCITSV+SA +AR+FHE+FSPIGS
Sbjct: 311 VGTQHLMLIIAMLLVSEYDGIYPCNHERISIDLVLMYCITSVVSASIARSFHEKFSPIGS 370

Query: 301 KECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAIRDL 360
           KECVFQTGALY FPVFIAV+LGKIFGI+TP+VD+ I  LLVAGFGSAI+IY+CCIA R++
Sbjct: 371 KECVFQTGALYFFPVFIAVILGKIFGISTPIVDSVICYLLVAGFGSAIMIYICCIAPRNI 430

Query: 361 YRPEHKTATCPTRRLLLYNRS---PSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           YRPE   ATC TR+LLLYNRS     TLWYMKTPAQM+L GLGIFLPISPLMD+IYASLW
Sbjct: 431 YRPERNAATCHTRKLLLYNRSSPPAPTLWYMKTPAQMMLEGLGIFLPISPLMDDIYASLW 490

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLK+C SFLTLFAAFLMV+L T                                      
Sbjct: 491 GLKICSSFLTLFAAFLMVVLTTFISGMALTSVQLLKNDYNWWWRSILRGGSPAIYMFGYG 550

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGY-TKKRS 488
                   +E+DR FVLPLVYNCCICYSFFLV GTVGFGASL+AFK+YM+G  TKKRS
Sbjct: 551 IYFISKIRSENDRGFVLPLVYNCCICYSFFLVFGTVGFGASLVAFKFYMMGCDTKKRS 608

BLAST of Lsi01G012240 vs. NCBI nr
Match: XP_023518444.1 (transmembrane 9 superfamily member 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.2 bits (1733), Expect = 3.5e-189
Identity = 348/537 (64.80%), Postives = 397/537 (73.93%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKR-RSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDD 60
           DA SYFSLPFCPPGE + KR +SLNEILAGDCLTNTQY+L+FGV  SEGFLC+KYMTEDD
Sbjct: 65  DADSYFSLPFCPPGEKLHKRKKSLNEILAGDCLTNTQYDLRFGVPVSEGFLCDKYMTEDD 124

Query: 61  LNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGI 120
           L RFK+AI NKFEYQMYF  IWFGSKVGE IE+TGLG+K+YLFNHIEFN+DFME+QV G+
Sbjct: 125 LKRFKTAIENKFEYQMYFADIWFGSKVGEAIEETGLGKKYYLFNHIEFNLDFMEDQVKGV 184

Query: 121 SVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNK----EASWVLEENRR 180
           ++VNSLDSSV ITN TET VEFSYSV WNEIKP +NSSYF   NK    +ASWVLEENR 
Sbjct: 185 NIVNSLDSSVAITNNTETPVEFSYSVVWNEIKPTNNSSYFKSWNKAPYGKASWVLEENRG 244

Query: 181 LFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLG 240
           LF S +WLW+ ++FWWV LPLVVASPYL  Y   NRQPH    R NDKVC CPMFTSLLG
Sbjct: 245 LFWSGIWLWTTISFWWVALPLVVASPYLFRYVTLNRQPHQG-SRFNDKVCQCPMFTSLLG 304

Query: 241 ATLGVGTQHLIIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSP 300
           A LGVGTQHLIIIVL F +AY GIYPC+  +ISVD++LAYC+TSV SAF+ R+FHE+FSP
Sbjct: 305 ALLGVGTQHLIIIVLLFVSAYQGIYPCNRERISVDIVLAYCLTSVPSAFIGRSFHEKFSP 364

Query: 301 IGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAI 360
           +  KECVF TGALY +P F+AVLL KIF   + MV++AIY L +AG GSAIL+YLCCIA 
Sbjct: 365 LKLKECVFLTGALYFYPAFLAVLLAKIFVSNSLMVNDAIYILSIAGIGSAILVYLCCIAT 424

Query: 361 RDLYRPEHKTATCPTRRLLLYNRSPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           R  Y PEH    CPTR + ++N   S+LWYMKTPAQM+L GL +FLPI  +MD+IYASLW
Sbjct: 425 RKWYEPEHDVTACPTRMVDVFNPPASSLWYMKTPAQMVLVGLSVFLPICLVMDDIYASLW 484

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLK CGSF TLFA F  VI+ TI                                     
Sbjct: 485 GLKACGSFFTLFAVFSTVIITTIISAYALTGVQLLKNDYNWWWRSVLRGGSPAILMFVYG 544

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGYTKKRS 488
                   TESDREF+  LVYNCCICYS FLVLGTV F AS  AF++YM    KKRS
Sbjct: 545 IYFLSKIKTESDREFLPLLVYNCCICYSLFLVLGTVSFRASHFAFRFYMGVVAKKRS 600

BLAST of Lsi01G012240 vs. NCBI nr
Match: XP_022963141.1 (transmembrane 9 superfamily member 5-like [Cucurbita moschata])

HSP 1 Score: 670.2 bits (1728), Expect = 1.3e-188
Identity = 346/537 (64.43%), Postives = 396/537 (73.74%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKR-RSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDD 60
           DA SYFSLPFCPPGE + KR +SLNEILAGDCLTNTQY+L+FGV  SEGFLC+KYMTEDD
Sbjct: 65  DADSYFSLPFCPPGEKLHKRKKSLNEILAGDCLTNTQYDLRFGVPVSEGFLCDKYMTEDD 124

Query: 61  LNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVIGI 120
           L RFK+AI NKFEYQMYF  IWFGSKVGE IE+TGLG+K+YLFNHIEFN+DFME+QV G+
Sbjct: 125 LKRFKTAIENKFEYQMYFADIWFGSKVGEAIEETGLGKKYYLFNHIEFNLDFMEDQVKGV 184

Query: 121 SVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPIHNSSYFIPVNK----EASWVLEENRR 180
           ++VNSLD SV ITN TET VEFSYSV WNEIKP +NSSYF   NK    +ASWVLEENR 
Sbjct: 185 NIVNSLDFSVAITNITETPVEFSYSVVWNEIKPTNNSSYFKSWNKAPYGKASWVLEENRG 244

Query: 181 LFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQPHGNVLRLNDKVCFCPMFTSLLG 240
           LF S +WLW+ ++FWWV LPLVVASPYL  Y   NRQPH    R NDKVC CPMFTSLLG
Sbjct: 245 LFWSGIWLWTTISFWWVALPLVVASPYLFRYVTLNRQPHQG-SRFNDKVCQCPMFTSLLG 304

Query: 241 ATLGVGTQHLIIIVLFFATAYDGIYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSP 300
           A LGVGTQHLIIIVL F +AY GIYPC+  +ISVD++LAYC+TSV SAF+ R+FHE+FSP
Sbjct: 305 ALLGVGTQHLIIIVLLFVSAYQGIYPCNRERISVDIVLAYCLTSVPSAFIGRSFHEKFSP 364

Query: 301 IGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNLLVAGFGSAILIYLCCIAI 360
           +  KECVF TGALY +P F+AVLL KIF   + MV++AIY L +AG GSAIL+YLCCIA 
Sbjct: 365 LKLKECVFLTGALYFYPAFLAVLLAKIFVSNSLMVNDAIYILSIAGIGSAILVYLCCIAT 424

Query: 361 RDLYRPEHKTATCPTRRLLLYNRSPSTLWYMKTPAQMILWGLGIFLPISPLMDEIYASLW 420
           R  Y PEH    C TR + ++N   S+LWYMKTPAQM+L GL +FLP+  +MD+IYASLW
Sbjct: 425 RKWYEPEHDVTACATRMVDVFNPPASSLWYMKTPAQMVLVGLSVFLPVCLVMDDIYASLW 484

Query: 421 GLKVCGSFLTLFAAFLMVILPTI------------------------------------- 480
           GLKVCGSF TLFA F  VI+ TI                                     
Sbjct: 485 GLKVCGSFFTLFAVFSTVIITTIISAYALTGVQLLKNDYNWWWRSVLRGGSPAILMFVYG 544

Query: 481 --------TESDREFVLPLVYNCCICYSFFLVLGTVGFGASLLAFKYYMIGYTKKRS 488
                   TESDREF+  LVYNCCICYS FLVLGTV F AS  AF++YM    KKRS
Sbjct: 545 IYFLSKIKTESDREFLPLLVYNCCICYSLFLVLGTVSFRASHFAFRFYMAVVAKKRS 600

BLAST of Lsi01G012240 vs. TAIR 10
Match: AT1G08350.2 (Endomembrane protein 70 protein family )

HSP 1 Score: 164.5 bits (415), Expect = 2.2e-40
Identity = 157/561 (27.99%), Postives = 244/561 (43.49%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y Y+ LPFC  G  + K+ +L E+L GD L ++ Y+LKF   ++   LC K +T  D+
Sbjct: 50  ETYQYYDLPFCRRGPVIEKQETLGEVLNGDRLMSSLYKLKFREDKTHFVLCRKRLTSSDI 109

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLGQ-----KFYLFNHIEFNVDFMENQ 120
            RF+  IA  + +QMY+D +     VG+V E    GQ     K+Y+F+H++FNV +  ++
Sbjct: 110 ARFRDIIAQDYYFQMYYDDLPLWGFVGKV-EGDYFGQGEKHTKYYIFSHLKFNVLYNADK 169

Query: 121 VIGISVVNSLDSSVDITNFTETLVEFSYSVFWN---EIKPIHNSSY----FIPVNKEASW 180
           VI I+  +     VDI+  TE  V+F+YSV WN   E      + Y    F P++++  +
Sbjct: 170 VIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLTSERSETRMNKYSRASFHPISQKIHF 229

Query: 181 VLEENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRN------------RQPHGN 240
                   FL+S+          V++ L+    +L    L+N            R+  G 
Sbjct: 230 F------SFLNSI---------TVVVLLIGLISFLFMRHLKNELRSYSIGDEEERKEAGW 289

Query: 241 VLRLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDG-IYPCDHVKISVDLILAY 300
            L ++  V  CP   S L A LG GTQ LI+I+  FA A+ G +YP +   +   L++ Y
Sbjct: 290 KL-VHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPYNRGMLLTSLVIMY 349

Query: 301 CITSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIY 360
            +TS+++ + + +FH +F     K  V   G LY  P FI + +     IT        Y
Sbjct: 350 TLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNTVAIT--------Y 409

Query: 361 NLLVA-GFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRSPSTL----------- 420
               A  FG+ ++I L    +   +           R  LL  + PS +           
Sbjct: 410 GATAALPFGTIVIIILIFTLLNIPFL--MLGGVLGNRFGLLEFQPPSAVKRNPREIPPQN 469

Query: 421 WYMKTPAQMILWGLGIFLPISPLMDEIYASLWGLKV------------------------ 478
           WY +   Q+ L G   F  +     ++YASLWG K+                        
Sbjct: 470 WYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGII 529

BLAST of Lsi01G012240 vs. TAIR 10
Match: AT5G37310.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 153.7 bits (387), Expect = 3.9e-37
Identity = 141/550 (25.64%), Postives = 237/550 (43.09%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC       K+ +L E+L GD L +  Y+L+F   ++    C K ++ +D+
Sbjct: 53  ETYRYFDLPFCSSAPVKEKKEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDV 112

Query: 61  NRFKSAIANKFEYQMYFDKIWFGSKVGEVIE--KTGLGQ-KFYLFNHIEFNVDFMENQVI 120
            +F+  IA  + +QMY+D +     +G+V++  KT   + K+YLFNH++F + + +++VI
Sbjct: 113 AKFRDVIAKDYYFQMYYDDLPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVI 172

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIK-------PIHNSSYFIPVNKEASWVL 180
            I V    +  VD+T   E  V+F+Y+V W E +         ++ +  +P + E  W  
Sbjct: 173 EIIVRTDQNFLVDLTEDKEVQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWF- 232

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   +  + +             HG+V 
Sbjct: 233 ----SIINSCVTVLLLTGFLATILMRVLKNDFV--KYAHDEEAVDDQEETGWKLIHGDVF 292

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SLL A LG GTQ   + V  F  A  G+ YP +   +   L++ Y +
Sbjct: 293 RF-------PKHKSLLAAALGSGTQLFTLAVFIFMLALVGVFYPYNRGALFTALVVIYAL 352

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + A +F+ +         V  TG+L+  P+ I         I         +  
Sbjct: 353 TSGIAGYTAASFYCQLEGTNWVRNVILTGSLFCGPLLITFSFLNTVAIAYQATAALPFGT 412

Query: 361 LVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRS-PSTLWYMKTPAQMILWG 420
           +V  F    L+    + +  +     K+      R   Y R  P   WY +T  QM + G
Sbjct: 413 IVVIFLIWALVTSPLLILGGIAGKNRKSEFQAPCRTTKYPREIPPMRWYRRTLPQMAMAG 472

Query: 421 LGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT-----------ITESD 478
              FLP S +  E+Y   AS+WG ++   +  L   FL++++ T           +   D
Sbjct: 473 ---FLPFSAIYIELYYIFASVWGHRIYTIYSILSIVFLILVIVTAFITVALTYFQLAAED 532

BLAST of Lsi01G012240 vs. TAIR 10
Match: AT1G14670.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 146.0 bits (367), Expect = 8.0e-35
Identity = 139/550 (25.27%), Postives = 231/550 (42.00%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC P     K+ +L E+L GD L +  Y+L F   +     C K ++++++
Sbjct: 52  ETYRYFDLPFCIPEGVKEKKEALGEVLNGDRLVSAPYKLNFRDEKESEVYCNKKLSKEEV 111

Query: 61  NRFKSAIANKFEYQMYFD--KIW-FGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVI 120
            +F+ A+   + +QMY+D   IW F  KV + I+      K++L+ HI+F + + +++VI
Sbjct: 112 KQFRKAVEKDYYFQMYYDDLPIWGFIGKVDKDIKSDPSEFKYFLYKHIQFEILYNKDRVI 171

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIK-------PIHNSSYFIPVNKEASWVL 180
            IS      S VD+T   E   EF Y+V W E +         ++ S  +P + E  W  
Sbjct: 172 EISARMDPHSLVDLTEDKEVDAEFMYTVKWKETETPFEKRMEKYSMSSSLPHHLEIHWF- 231

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   + ++ +             HG+V 
Sbjct: 232 ----SIINSCVTVLLLTGFLATILMRVLKNDFM--KYAQDEEAADDQEETGWKYIHGDVF 291

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SL  A+LG GTQ   + +  F  A  G+ YP +   +   L++ Y +
Sbjct: 292 RF-------PTHNSLFAASLGSGTQLFTLTIFIFMLALVGVFYPYNRGALFTALVVIYAL 351

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + + +F+ +         +  TG L+  P+F+         IT        +  
Sbjct: 352 TSGIAGYTSASFYCQLEGKSWVRNLLLTGCLFCGPLFLTFCFLNTVAITYTATAALPFGT 411

Query: 361 LVAGFGSAILIYLCCIAIRDLYRPEHKTATCPTRRLLLYNRS-PSTLWYMKTPAQMILWG 420
           +V       L+    + +  +     K       R   Y R  P   WY     QM + G
Sbjct: 412 IVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPWYRSAIPQMAMAG 471

Query: 421 LGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT--ITESDREFVLP--- 478
              FLP S +  E+Y   AS+WG ++   +  LF  F+++I+ T  IT +   F L    
Sbjct: 472 ---FLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILIIVTAFITVALTYFQLAAED 531

BLAST of Lsi01G012240 vs. TAIR 10
Match: AT2G01970.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-32
Identity = 137/558 (24.55%), Postives = 229/558 (41.04%), Query Frame = 0

Query: 1   DAYSYFSLPFCPPGEGVSKRRSLNEILAGDCLTNTQYELKFGVSESEGFLCEKYMTEDDL 60
           + Y YF LPFC P     K+ +L E+L GD L +  Y+L F   +     C+K ++ +++
Sbjct: 52  ETYRYFDLPFCIPEGVKDKKEALGEVLNGDRLVSAPYKLNFRDEKDSEVYCKKKLSREEV 111

Query: 61  NRFKSAIANKFEYQMYFD--KIW-FGSKVGEVIEKTGLGQKFYLFNHIEFNVDFMENQVI 120
             F+ A+   + +QMY+D   IW F  KV +  +      K++L+ HI+F + + +++VI
Sbjct: 112 EHFRRAVEKDYYFQMYYDDLPIWGFIGKVDKESKSDPSEFKYFLYKHIQFEILYNKDRVI 171

Query: 121 GISVVNSLDSSVDITNFTETLVEFSYSVFWNEIKPI-------HNSSYFIPVNKEASWVL 180
            I+      S VD+T   E   EF Y+V W E +         +  S  +P + E  W  
Sbjct: 172 EINARMDPHSLVDLTEDKEVDAEFMYTVKWKETETSFEKRMDKYAMSSSLPHHLEIHWF- 231

Query: 181 EENRRLFLSSLWLWSIMAFWWVILPLVVASPYLVPYFLRNRQP------------HGNVL 240
                +  S + +  +  F   IL  V+ + ++   + ++ +             HG+V 
Sbjct: 232 ----SIINSCVTVLLLTGFLATILMRVLKNDFM--KYAQDEEAADDQEETGWKYIHGDVF 291

Query: 241 RLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFFATAYDGI-YPCDHVKISVDLILAYCI 300
           R        P   SL  A+LG GTQ   + +  F  +  G+ YP +   +   L++ Y +
Sbjct: 292 RF-------PKNKSLFAASLGSGTQLFTLTIFIFMLSLVGVFYPYNRGALFTALVVIYAL 351

Query: 301 TSVLSAFMARAFHEEFSPIGSKECVFQTGALYLFPVFIAVLLGKIFGITTPMVDNAIYNL 360
           TS ++ + A +F+ +         +  TG L+  P+F+         I         Y+ 
Sbjct: 352 TSGIAGYTASSFYCQLEGKNWVRNLLLTGGLFCGPLFLTFCFLNTVAIA--------YSA 411

Query: 361 LVA-GFGSAILIYLCCIAIRD--------LYRPEHKTATCPTRRLLLYNRSPSTLWYMKT 420
             A  FG+ I+I L    +            +        P R        P   WY   
Sbjct: 412 TAALPFGTIIVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPVRTTKYPREIPPLPWYRSA 471

Query: 421 PAQMILWGLGIFLPISPLMDEIY---ASLWGLKVCGSFLTLFAAFLMVILPT-------- 478
             QM + G   FLP S +  E+Y   AS+WG ++   +  LF  F+++++ T        
Sbjct: 472 VPQMAMAG---FLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITVALT 531

BLAST of Lsi01G012240 vs. TAIR 10
Match: AT1G08350.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-30
Identity = 143/525 (27.24%), Postives = 222/525 (42.29%), Query Frame = 0

Query: 37  YELKFGVSESEGFLCEKYMTEDDLNRFKSAIANKFEYQMYFDKIWFGSKVGEVIEKTGLG 96
           Y+LKF   ++   LC K +T  D+ RF+  IA  + +QMY+D +     VG+V E    G
Sbjct: 5   YKLKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKV-EGDYFG 64

Query: 97  Q-----KFYLFNHIEFNVDFMENQVIGISVVNSLDSSVDITNFTETLVEFSYSVFWN--- 156
           Q     K+Y+F+H++FNV +  ++VI I+  +     VDI+  TE  V+F+YSV WN   
Sbjct: 65  QGEKHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLTS 124

Query: 157 EIKPIHNSSY----FIPVNKEASWVLEENRRLFLSSLWLWSIMAFWWVILPLVVASPYLV 216
           E      + Y    F P++++  +        FL+S+          V++ L+    +L 
Sbjct: 125 ERSETRMNKYSRASFHPISQKIHFF------SFLNSI---------TVVVLLIGLISFLF 184

Query: 217 PYFLRN------------RQPHGNVLRLNDKVCFCPMFTSLLGATLGVGTQHLIIIVLFF 276
              L+N            R+  G  L ++  V  CP   S L A LG GTQ LI+I+  F
Sbjct: 185 MRHLKNELRSYSIGDEEERKEAGWKL-VHSDVFRCPRNISWLCAILGTGTQLLILIIALF 244

Query: 277 ATAYDG-IYPCDHVKISVDLILAYCITSVLSAFMARAFHEEFSPIGSKECVFQTGALYLF 336
           A A+ G +YP +   +   L++ Y +TS+++ + + +FH +F     K  V   G LY  
Sbjct: 245 ALAFTGFLYPYNRGMLLTSLVIMYTLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPV 304

Query: 337 PVFIAVLLGKIFGITTPMVDNAIYNLLVA-GFGSAILIYLCCIAIRDLYRPEHKTATCPT 396
           P FI + +     IT        Y    A  FG+ ++I L    +   +           
Sbjct: 305 PFFIILSVLNTVAIT--------YGATAALPFGTIVIIILIFTLLNIPFL--MLGGVLGN 364

Query: 397 RRLLLYNRSPSTL-----------WYMKTPAQMILWGLGIFLPISPLMDEIYASLWGLKV 456
           R  LL  + PS +           WY +   Q+ L G   F  +     ++YASLWG K+
Sbjct: 365 RFGLLEFQPPSAVKRNPREIPPQNWYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKI 424

Query: 457 -------------------------------------------CGSFLTLFAAFLMVILP 478
                                                      CG F  +F     V+  
Sbjct: 425 YTSPGIMLFTFIVLIFLSSSVGIILTYIQLSGEDHEWWWRSILCGGFTAVFMYGYGVLF- 484