Lsi01G011970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G011970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr01: 10225594 .. 10226685 (+)
RNA-Seq Expression	Lsi01G011970
Synteny	Lsi01G011970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGATTCAGACTCATGGATGAATCCTGTTCTTCCAAAAGGTGAATTTACCTTCCCTCTCTTTCACAGCTATATATATGAACCAATCACGAAAAACCTCTGTTTCATTCCATTTTGCAGGTTGAATGGCAAAGTAGCTATAATCACCGGCGGCGCCAGCGGATTCGGCAAGAGTACCGCCAGGTTGTTTGTTCGACACGGCGCCAGAGTGGTCATCGCCGACGTCCAGGAAGATCTCGGCCGATCACTCTGCAAAGAACTCGGATCGGAGAAATCTGTTTCGTACGTACATTGCGACGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTACGGGAAGCTGGACATAATGTACAACAATGCCGGAATTACCGGCAAAATGGATCCGACGATTTTAGGAACAGATGGAGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTGTACGGAGGATTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAATAGGAGCGGAGTGATTCTGTTCACATCTAGTGTTGCTTCGGTGAATAGTGGAGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGGATTAGGGTTAATTCTGTATCTCCGGGGGCTATTGCAACGCCGTTGCTGAGAAATGCGTTGGGATTCACGGAGGAGGAGTTGGAGGAGGTAGTTCTCTCGTCGGCGATCTTGAAAGGGGTGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTTTACTTGTGTAGTGATGAATCTAGGGTTATAAGCGGGCATAATCTCGTCGTCGACGGTGGCTACAGTACGGCCAACCGCTCTTTCTCCTCTGCCGCTATTAGAAAATTGTCCTCTGTATTAGAGGGTAGAAATTAGGGCTTATCTTTGTGTCTCCATTCGAAAATATTTTCACATAACTAAAAGATTTTTTCTTTTTGATTGGATAATAGCTTCTCATTTCAAGGTTGTCAGTATAGAAGTAAGAAAAATACTTAGGTACATCTTCAATCTAATTATATTTTGACACGTGG

mRNA sequence

ATTTGATTCAGACTCATGGATGAATCCTGTTCTTCCAAAAGGTTGAATGGCAAAGTAGCTATAATCACCGGCGGCGCCAGCGGATTCGGCAAGAGTACCGCCAGGTTGTTTGTTCGACACGGCGCCAGAGTGGTCATCGCCGACGTCCAGGAAGATCTCGGCCGATCACTCTGCAAAGAACTCGGATCGGAGAAATCTGTTTCGTACGTACATTGCGACGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTACGGGAAGCTGGACATAATGTACAACAATGCCGGAATTACCGGCAAAATGGATCCGACGATTTTAGGAACAGATGGAGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTGTACGGAGGATTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAATAGGAGCGGAGTGATTCTGTTCACATCTAGTGTTGCTTCGGTGAATAGTGGAGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGGATTAGGGTTAATTCTGTATCTCCGGGGGCTATTGCAACGCCGTTGCTGAGAAATGCGTTGGGATTCACGGAGGAGGAGTTGGAGGAGGTAGTTCTCTCGTCGGCGATCTTGAAAGGGGTGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTTTACTTGTGTAGTGATGAATCTAGGGTTATAAGCGGGCATAATCTCGTCGTCGACGGTGGCTACAGTACGGCCAACCGCTCTTTCTCCTCTGCCGCTATTAGAAAATTGTCCTCTGTATTAGAGGGTAGAAATTAGGGCTTATCTTTGTGTCTCCATTCGAAAATATTTTCACATAACTAAAAGATTTTTTCTTTTTGATTGGATAATAGCTTCTCATTTCAAGGTTGTCAGTATAGAAGTAAGAAAAATACTTAGGTACATCTTCAATCTAATTATATTTTGACACGTGG

Coding sequence (CDS)

ATGGATGAATCCTGTTCTTCCAAAAGGTTGAATGGCAAAGTAGCTATAATCACCGGCGGCGCCAGCGGATTCGGCAAGAGTACCGCCAGGTTGTTTGTTCGACACGGCGCCAGAGTGGTCATCGCCGACGTCCAGGAAGATCTCGGCCGATCACTCTGCAAAGAACTCGGATCGGAGAAATCTGTTTCGTACGTACATTGCGACGTGACGAGCGATTCCGATGTGAAGAACGCCGTCGATTTCGCCGTCGAGAGGTACGGGAAGCTGGACATAATGTACAACAATGCCGGAATTACCGGCAAAATGGATCCGACGATTTTAGGAACAGATGGAGAGAATTTCAAGAAGGTTTTCGAGGTGAATGTGTACGGAGGATTTTTAGGAGCGAAACATGCGGCTAGGGTTATGATTCCGAATAGGAGCGGAGTGATTCTGTTCACATCTAGTGTTGCTTCGGTGAATAGTGGAGAATCGCCGCATGCTTATGCAATGTCGAAGCACGCGGTGGTGGGATTGATGAGGAATTTGTGTGTGGAATTGGGGGAATTTGGGATTAGGGTTAATTCTGTATCTCCGGGGGCTATTGCAACGCCGTTGCTGAGAAATGCGTTGGGATTCACGGAGGAGGAGTTGGAGGAGGTAGTTCTCTCGTCGGCGATCTTGAAAGGGGTGGTGCCGACGGCGGAGGATGTGGCGGAGGCGGCGCTTTACTTGTGTAGTGATGAATCTAGGGTTATAAGCGGGCATAATCTCGTCGTCGACGGTGGCTACAGTACGGCCAACCGCTCTTTCTCCTCTGCCGCTATTAGAAAATTGTCCTCTGTATTAGAGGGTAGAAATTAG

Protein sequence

MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCSDESRVISGHNLVVDGGYSTANRSFSSAAIRKLSSVLEGRN

Homology

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BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.0e-75
Identity = 139/263 (52.85%), Postives = 187/263 (71.10%), Query Frame = 0


Query: 6   SSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYV 65
           S+ RL  KVAIITGGA G G++TA+LFVR+GA+VVIAD+ +D G+ +C  +GS   +S+V
Sbjct: 10  STNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFV 69

Query: 66  HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGG 125
           HCDVT D DV+N VD  + ++GKLDIM+ N G+      +IL    E+FK+V ++NVYG 
Sbjct: 70  HCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGA 129

Query: 126 FLGAKHAARVMIPNRSGVILFTSSVASVNSGES-PHAYAMSKHAVVGLMRNLCVELGEFG 185
           FL AKHAARVMIP + G I+FT+S++S  +GE   H Y  +KHAV+GL  +LC ELGE+G
Sbjct: 130 FLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYG 189

Query: 186 IRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCSDESR 245
           IRVN VSP  +A+PLL +  G     +EE+   +A LKG +  AEDVA+A  YL  DES+
Sbjct: 190 IRVNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDESK 249

Query: 246 VISGHNLVVDGGYSTANRSFSSA 268
            +SG NLV+DGGY+  N +F +A
Sbjct: 250 YVSGLNLVIDGGYTRTNPAFPTA 272

[Close]

BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.4e-75
Identity = 138/263 (52.47%), Postives = 187/263 (71.10%), Query Frame = 0


Query: 6   SSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYV 65
           S+ RL  KVAIITGGA G G++TA+LFVR+GA+VVIAD+ +D G+ +C  +GS   +S+V
Sbjct: 10  STNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFV 69

Query: 66  HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGG 125
           HCDVT D DV+N VD  + ++GKLDIM+ N G+      +IL    E+FK+V ++NVYG 
Sbjct: 70  HCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGA 129

Query: 126 FLGAKHAARVMIPNRSGVILFTSSVASVNSGES-PHAYAMSKHAVVGLMRNLCVELGEFG 185
           FL AKHAARVMIP + G I+FT+S++S  +GE   H Y  +KHAV+GL  +LC ELG+ G
Sbjct: 130 FLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHG 189

Query: 186 IRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCSDESR 245
           IRVN VSP  +A+PLL +  G     +EE+   +A LKG++  AEDVA+A  YL  DES+
Sbjct: 190 IRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDESK 249

Query: 246 VISGHNLVVDGGYSTANRSFSSA 268
            +SG NLV+DGGY+  N +F +A
Sbjct: 250 YVSGLNLVIDGGYTRTNPAFPTA 272

[Close]

BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.3e-73
Identity = 145/262 (55.34%), Postives = 185/262 (70.61%), Query Frame = 0


Query: 4   SCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVS 63
           S  +++L GKVA+ITGGASG G  TARLFV+HGARVV+AD+Q++LG SL  ELG + S S
Sbjct: 9   SADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDAS-S 68

Query: 64  YVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVY 123
           YVHCDVT++ DV  AVD AV R+GKLD+M+NNAG++G     +     E+F++V  VN+ 
Sbjct: 69  YVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLV 128

Query: 124 GGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEF 183
           G FLG KHAARVM P R G I+ T+S++S  SG + HAY  SKHA+VG   N   ELG  
Sbjct: 129 GPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRH 188

Query: 184 GIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGV-VPTAEDVAEAALYLCSDE 243
           GIRVN VSP  +ATPL R A+G  +E +E ++ +SA LKG     A+D+A AAL+L SD+
Sbjct: 189 GIRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDD 248

Query: 244 SRVISGHNLVVDGGYSTANRSF 265
            R +SG NL VDGG S  N SF
Sbjct: 249 GRYVSGQNLRVDGGLSVVNSSF 269

[Close]

BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.7e-72
Identity = 143/256 (55.86%), Postives = 185/256 (72.27%), Query Frame = 0


Query: 7   SKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYVH 66
           ++RL GKVA+ITGGASG G++TA+LF +HGA+V IADVQ++LG S+ + +G+  S +Y+H
Sbjct: 12  ARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS-TYIH 71

Query: 67  CDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGGF 126
           CDVT++  VKNAVD  V  YGKLDIM++NAGI+    P I+  +  +F++VF VNV G F
Sbjct: 72  CDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVF 131

Query: 127 LGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIR 186
           L  KHAARVMIP RSG I+ T+S++S   G S HAY  SKHAV+GL RNL VELG+FGIR
Sbjct: 132 LCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIR 191

Query: 187 VNSVSPGAIATPLLRNALGF-TEEELEEVVLSSAILKGVVPTAEDVAEAALYLCSDESRV 246
           VN +SP  + T L +   G   EEE E V+  +  LKG     EDVA AALYL SDE++ 
Sbjct: 192 VNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKY 251

Query: 247 ISGHNLVVDGGYSTAN 262
           +SGHNL +DGG+S  N
Sbjct: 252 VSGHNLFIDGGFSVCN 266

[Close]

BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.0e-68
Identity = 138/263 (52.47%), Postives = 176/263 (66.92%), Query Frame = 0


Query: 9   RLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYVHCD 68
           RL GKVA++TGGASG G+S ARLF+ HGA++ I DVQ++LG+ + + LG +    Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 69  VTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGGFLG 128
           VT + DV+ AVDF  E+YG +DIM NNAGITG     I   D   FKKVF++NV G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 129 AKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGIRVN 188
            KHAAR+MIP   G I+  +SV+SV +G  PH Y  +KHAVVGL +++  ELG  GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 189 SVSPGAIATPLLRNALGFTEEELEE--------VVLSSAILKGVVPTAEDVAEAALYLCS 248
            VSP A+ T L    L   E E++E         V S+A LKGV     DVAEA LYL +
Sbjct: 182 CVSPYAVPTRLSMPYL--PESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLAT 241

Query: 249 DESRVISGHNLVVDGGYSTANRS 264
           +ES+ +SG NLV+DGG+S AN +
Sbjct: 242 EESKYVSGLNLVIDGGFSIANHT 262

[Close]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL
Match: A0A6J1IPT5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478837 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 7.0e-140
Identity = 257/274 (93.80%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV ++LGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEEVV SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL
Match: A0A6J1EDN5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433246 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.6e-139
Identity = 255/274 (93.07%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV +DLGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSY+HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYLHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSG+ILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEEV+ SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL
Match: A0A5A7SIU8 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001660 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 255/279 (91.40%), Postives = 269/279 (96.42%), Query Frame = 0


Query: 1   MDES-CSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSE 60
           MD+S  SSKRLNGKVA+ITG ASGFGKSTARLFV+HGARVV+ADVQ++L + LCKELGSE
Sbjct: 1   MDQSMISSKRLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSE 60

Query: 61  KSVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFE 120
           +SVSY+HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFE
Sbjct: 61  ESVSYIHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFE 120

Query: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180
           VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE
Sbjct: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180

Query: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLC 240
           LGEFGIRVNSVSPGAIATPLLRNALGFT++ELEEVV SSAILKGVV TAEDVAEAALYLC
Sbjct: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLC 240

Query: 241 SDESRVISGHNLVVDGGYSTANRSFSSAAIRKLSSVLEG 279
           SDESRVISGHNLVVDGGYSTANRSFS+A IRK+SS  EG
Sbjct: 241 SDESRVISGHNLVVDGGYSTANRSFSAAVIRKVSSAFEG 279

[Close]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL
Match: A0A1S3CHD5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.9e-137
Identity = 255/279 (91.40%), Postives = 269/279 (96.42%), Query Frame = 0


Query: 1   MDES-CSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSE 60
           MD+S  SSKRLNGKVA+ITG ASGFGKSTARLFV+HGARVV+ADVQ++L + LCKELGSE
Sbjct: 1   MDQSMISSKRLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSE 60

Query: 61  KSVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFE 120
           +SVSY+HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFE
Sbjct: 61  ESVSYIHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGQMDPTILGTDGENFKKVFE 120

Query: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180
           VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE
Sbjct: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180

Query: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLC 240
           LGEFGIRVNSVSPGAIATPLLRNALGFT++ELEEVV SSAILKGVV TAEDVAEAALYLC
Sbjct: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTKKELEEVVRSSAILKGVVATAEDVAEAALYLC 240

Query: 241 SDESRVISGHNLVVDGGYSTANRSFSSAAIRKLSSVLEG 279
           SDESRVISGHNLVVDGGYSTANRSFS+A IRK+SS  EG
Sbjct: 241 SDESRVISGHNLVVDGGYSTANRSFSAAVIRKVSSAFEG 279

[Close]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL
Match: A0A0A0KFF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454360 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.2e-134
Identity = 251/279 (89.96%), Postives = 266/279 (95.34%), Query Frame = 0


Query: 1   MDES-CSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSE 60
           MD+S  SSKRLNGKVA+ITG ASGFGKSTARLFV+HGARVV+ADVQ++L + LCKELGSE
Sbjct: 1   MDQSTISSKRLNGKVALITGAASGFGKSTARLFVQHGARVVLADVQDNLAQPLCKELGSE 60

Query: 61  KSVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFE 120
           ++VSY+HCDVT DSDVK AVDFAVERYGKLDIMYNNAGITG+MDPTILGT+GENFKKVFE
Sbjct: 61  ETVSYIHCDVTRDSDVKEAVDFAVERYGKLDIMYNNAGITGQMDPTILGTNGENFKKVFE 120

Query: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180
           VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE
Sbjct: 121 VNVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVE 180

Query: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLC 240
           LGEFGIRVNSVSPGAIATPLLRNALGFTE+ELEEVV SSAILKGVV TAEDVAEAALYLC
Sbjct: 181 LGEFGIRVNSVSPGAIATPLLRNALGFTEKELEEVVRSSAILKGVVATAEDVAEAALYLC 240

Query: 241 SDESRVISGHNLVVDGGYSTANRSFSSAAIRKLSSVLEG 279
           SDESRVISGHNLVVDGGYSTANRSFS+A I KLSS  +G
Sbjct: 241 SDESRVISGHNLVVDGGYSTANRSFSAAVITKLSSDFQG 279

[Close]

BLAST of Lsi01G011970 vs. NCBI nr
Match: XP_038882752.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 509.2 bits (1310), Expect = 2.2e-140
Identity = 263/278 (94.60%), Postives = 269/278 (96.76%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDES SSKRLNGKVAIITGGASGFGKSTARLFVRHGA VVIADVQ+DLGRSLC+ELGSEK
Sbjct: 1   MDESYSSKRLNGKVAIITGGASGFGKSTARLFVRHGATVVIADVQDDLGRSLCEELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSYVHCDVTSDSDVKNAVDFAVERYG+LDIMYNNAGITG+MDPTILGT  ENFKKVFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGQLDIMYNNAGITGEMDPTILGTKKENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNSVSPGAIATPLLRNALGFTEE LEEVV SSAILKGVVPT EDVAEAAL+LCS
Sbjct: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTVEDVAEAALFLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSSVLEG 279
           DESRVISGHNLVVDGGYSTANRSFS AAIRKLSS LEG
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSVAAIRKLSSHLEG 278

[Close]

BLAST of Lsi01G011970 vs. NCBI nr
Match: KAG6604329.1 (hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 508.1 bits (1307), Expect = 5.0e-140
Identity = 258/274 (94.16%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV +DLGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEEVV SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. NCBI nr
Match: XP_022979096.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 506.5 bits (1303), Expect = 1.4e-139
Identity = 257/274 (93.80%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV ++LGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDELGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEEVV SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. NCBI nr
Match: XP_023543891.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.5e-139
Identity = 256/274 (93.43%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV +DLGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSYVHCDVTSDSDVKN+VDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYVHCDVTSDSDVKNSVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSGVILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGVILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEE+V SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEELVRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. NCBI nr
Match: XP_022925996.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 505.4 bits (1300), Expect = 3.2e-139
Identity = 255/274 (93.07%), Postives = 266/274 (97.08%), Query Frame = 0


Query: 1   MDESCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEK 60
           MDESCSSKRLNGKVAIITG ASGFGKSTARLFV+HGA VV+ADV +DLGRSLC+ELGSEK
Sbjct: 1   MDESCSSKRLNGKVAIITGAASGFGKSTARLFVKHGASVVVADVLDDLGRSLCQELGSEK 60

Query: 61  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 120
           +VSY+HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG+MDPTILGTDGENFKKVFEV
Sbjct: 61  TVSYLHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGEMDPTILGTDGENFKKVFEV 120

Query: 121 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 180
           NVYGGFLGAKHAARVMIP RSG+ILFTSSVASVNSGESPHAY MSKHAVVGLMRNLCVEL
Sbjct: 121 NVYGGFLGAKHAARVMIPRRSGLILFTSSVASVNSGESPHAYTMSKHAVVGLMRNLCVEL 180

Query: 181 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 240
           GEFGIRVNS+SPGAIATPLLRNALGFTEE LEEV+ SSAILKGVVPTAEDVAEAALYLCS
Sbjct: 181 GEFGIRVNSISPGAIATPLLRNALGFTEEALEEVIRSSAILKGVVPTAEDVAEAALYLCS 240

Query: 241 DESRVISGHNLVVDGGYSTANRSFSSAAIRKLSS 275
           DESRVISGHNLVVDGGYSTANRSFS+A IRKLSS
Sbjct: 241 DESRVISGHNLVVDGGYSTANRSFSAAVIRKLSS 274

[Close]

BLAST of Lsi01G011970 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.1e-68
Identity = 136/259 (52.51%), Postives = 176/259 (67.95%), Query Frame = 0


Query: 4   SCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVS 63
           +C+SK+L GKVA+ITGGASG GK+TA  F+RHGARVVIAD+  + G    KELGSE    
Sbjct: 35  TCTSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSE--AE 94

Query: 64  YVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDP-TILGTDGENFKKVFEVNV 123
           +V CDVT ++D+  AV+  VERYGKLD+MYNNAGI G M P +I   D   F++V  +NV
Sbjct: 95  FVRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINV 154

Query: 124 YGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGE 183
           +G   G KHAA+ MIP RSG IL TSSVA V  G +PH+Y +SK    G++++   EL E
Sbjct: 155 FGVVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCE 214

Query: 184 FGIRVNSVSPGAIATPLLRNAL-----GFTEEELEEVVLSSAILKGVVPTAEDVAEAALY 243
            G+R+N +SPG +ATPL  + L       +EE+L E V     LKG      DVA+AALY
Sbjct: 215 HGVRINCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALY 274

Query: 244 LCSDESRVISGHNLVVDGG 257
           L S++ + ++GHNLVVDGG
Sbjct: 275 LASNDGKYVTGHNLVVDGG 291

[Close]

BLAST of Lsi01G011970 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.2e-67
Identity = 144/268 (53.73%), Postives = 175/268 (65.30%), Query Frame = 0


Query: 8   KRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKS---VSY 67
           KRL GKVAIITGGA G GK+T  LF RHGA VVIADV    G SL K L S K+   V++
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 68  VHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITG--KMDPTILGTDGENFKKVFEVNV 127
           + CDV+ ++DV+N V+  V RYG+LDI++NNAG+ G  K   +IL  D + F  V  VNV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 128 YGGFLGAKHAARVMIPNR-SGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELG 187
            G  LG KH AR MI     G I+ T+SVA V  G  PHAY  SKHA+VGL +N   ELG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 188 EFGIRVNSVSPGAIATPLLRNALGFTE---------EELEEVVLSSAILKGVVPTAEDVA 247
           ++GIRVN +SP  +AT +L NA   T          EE+EE V S A LKG    A D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 248 EAALYLCSDESRVISGHNLVVDGGYSTA 261
           EAALYL SDES+ ++GHNLVVDGG +TA
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGGVTTA 297

[Close]

BLAST of Lsi01G011970 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-64
Identity = 135/271 (49.82%), Postives = 176/271 (64.94%), Query Frame = 0


Query: 4   SCSSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKEL---GSEK 63
           S  S+RL GKVA+ITGGA+G G+S  RLF +HGA+V I D+Q+DLG  +CK L    S++
Sbjct: 12  SLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKE 71

Query: 64  SVSYVHCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEV 123
           +  ++H DV  + D+ NAVDFAV+ +G LDI+ NNAG+ G   P I       F+  F+V
Sbjct: 72  TAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDV 131

Query: 124 NVYGGFLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVEL 183
           NV G FL  KHAARVMIP + G I+   SV  V  G  PH+Y  SKHAV+GL R++  EL
Sbjct: 132 NVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAEL 191

Query: 184 GEFGIRVNSVSPGAIATPLLRNALGFTEEELEEVVL-------SSAILKGVVPTAEDVAE 243
           G+ GIRVN VSP A+AT L    L   EE  E+  +       ++A LKGV  T +DVA 
Sbjct: 192 GQHGIRVNCVSPYAVATKLALAHLP-EEERTEDAFVGFRNFAAANANLKGVELTVDDVAN 251

Query: 244 AALYLCSDESRVISGHNLVVDGGYSTANRSF 265
           A L+L SD+SR ISG NL++DGG++  N SF
Sbjct: 252 AVLFLASDDSRYISGDNLMIDGGFTCTNHSF 281

[Close]

BLAST of Lsi01G011970 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 8.1e-64
Identity = 129/259 (49.81%), Postives = 172/259 (66.41%), Query Frame = 0


Query: 6   SSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYV 65
           +S++L GKVA+ITGGASG GK+TA  F+ HGA+V+IAD+Q  +GR   +ELG   S +Y 
Sbjct: 74  TSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELG--PSCAYF 133

Query: 66  HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDPTILGTDGENFKKVFEVNVYGG 125
            CDVT +SD+ NAVDFAV  + KLDIMYNNAGI  K  P+I+  D   F KV   NV G 
Sbjct: 134 PCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGV 193

Query: 126 FLGAKHAARVMIPNRSGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGEFGI 185
             G KHAARVMIP  SG I+   SV  +  G + H Y++SK AV+G++R+   EL +  I
Sbjct: 194 MAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRI 253

Query: 186 RVNSVSPGAIATPLLRNAL-----GFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCS 245
           RVN +SP AI T  + + +     G  +  L ++V S+ +L G V    DVA AA+YL S
Sbjct: 254 RVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLAS 313

Query: 246 DESRVISGHNLVVDGGYST 260
           D+S+ ++GHNLVVDGG++T
Sbjct: 314 DDSKYVNGHNLVVDGGFTT 330

[Close]

BLAST of Lsi01G011970 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 6.4e-61
Identity = 123/256 (48.05%), Postives = 174/256 (67.97%), Query Frame = 0


Query: 6   SSKRLNGKVAIITGGASGFGKSTARLFVRHGARVVIADVQEDLGRSLCKELGSEKSVSYV 65
           S KRL+GK+ IITGGASG G  + RLF  HGARVVI DVQ++LG+++   +G +K+ SY 
Sbjct: 2   SGKRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKA-SYY 61

Query: 66  HCDVTSDSDVKNAVDFAVERYGKLDIMYNNAGITGKMDP--TILGTDGENFKKVFEVNVY 125
           HCDVT++++V+NAV F VE+YGKLD++++NAG+   ++P  +IL  +     +   +N+ 
Sbjct: 62  HCDVTNETEVENAVKFTVEKYGKLDVLFSNAGV---IEPFVSILDLNLNELDRTIAINLR 121

Query: 126 GGFLGAKHAARVMIPNR-SGVILFTSSVASVNSGESPHAYAMSKHAVVGLMRNLCVELGE 185
           G     KHAAR M+     G I+ T+SVA+  +G +PH Y  SKH ++GL+++    LG+
Sbjct: 122 GTAAFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGK 181

Query: 186 FGIRVNSVSPGAIATPLLRNALGFTEEELEEVVLSSAILKGVVPTAEDVAEAALYLCSDE 245
           +GIRVN V+P  +ATPL+ N        +E+   +SA LKG+V  A  VAEAAL+L SDE
Sbjct: 182 YGIRVNGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDE 241

Query: 246 SRVISGHNLVVDGGYS 259
           S  +SG NL VDGGYS
Sbjct: 242 SAYVSGQNLAVDGGYS 253

The following BLAST results are available for this feature:

BLAST of Lsi01G011970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL8	2.0e-75	52.85	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	3.4e-75	52.47	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q7FAE1	9.3e-73	55.34	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL7	2.7e-72	55.86	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	4.0e-68	52.47	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of Lsi01G011970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IPT5	7.0e-140	93.80	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1EDN5	1.6e-139	93.07	secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A5A7SIU8	1.9e-137	91.40	Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CHD5	1.9e-137	91.40	secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103500923 ...	[more]
A0A0A0KFF0	1.2e-134	89.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454360 PE=4 SV=1	[more]

BLAST of Lsi01G011970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882752.1	2.2e-140	94.60	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
KAG6604329.1	5.0e-140	94.16	hypothetical protein SDJN03_04938, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022979096.1	1.4e-139	93.80	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]
XP_023543891.1	2.5e-139	93.43	secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022925996.1	3.2e-139	93.07	secoisolariciresinol dehydrogenase-like [Cucurbita moschata]	[more]

BLAST of Lsi01G011970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26770.1	1.1e-68	52.51	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	1.2e-67	53.73	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	1.2e-64	49.82	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	8.1e-64	49.81	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	6.4e-61	48.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 162..181 score: 33.69 coord: 142..150 score: 44.98 coord: 87..98 score: 50.98
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 14..31 score: 45.77 coord: 183..200 score: 48.36 coord: 162..181 score: 38.45 coord: 221..241 score: 30.29 coord: 87..98 score: 57.66 coord: 136..152 score: 30.85
None	No IPR available	GENE3D	3.40.50.720		coord: 2..273 e-value: 2.5E-86 score: 291.1
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 19..258 e-value: 3.0E-69 score: 233.2
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 16..212 e-value: 1.5E-21 score: 74.7
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 9..261 e-value: 1.4E-25 score: 87.8
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 5..269
None	No IPR available	PANTHER	PTHR43180:SF45	SECOISOLARICIRESINOL DEHYDROGENASE-RELATED	coord: 5..269
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 11..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G011970.1	Lsi01G011970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0055041	cyclopentanol dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity