Lsi01G011480 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G011480 vs. ExPASy Swiss-Prot
Match: F4KF14 (Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSHI4 PE=1 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 636/858 (74.13%), Postives = 726/858 (84.62%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M F  S++L     S P+ PS T        S  S  R    +  K  S+   F     R
Sbjct: 1   MTFYISSSLTPTHFSKPLNPSNTLFPSQFRGSLSSFVRRRKPTEAKLSSK---FNLFPSR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           ++ + T   S+S  ++  +S + E D ES +LFEKL++ ER+R++ +EEL+RKAN+QLER
Sbjct: 61  RNGLIT-CCSTSSFESTESSVSQEEDAESNRLFEKLRETERERLSNMEELERKANVQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMAS WSR LLTMRGKLKGTEWDPE SHRINFSDF  LL+S++VQ++EYSNYGQT+SV
Sbjct: 121 QLVMASDWSRTLLTMRGKLKGTEWDPETSHRINFSDFMKLLDSNSVQYMEYSNYGQTISV 180

Query: 181 ILPYYKD-ETGG---SAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPA 240
           ILPYYKD E  G    +K EIIFRRH++DRMPID WNDVWKKLHQQIVNV+V NVD VPA
Sbjct: 181 ILPYYKDGEPLGEEEDSKKEIIFRRHIVDRMPIDGWNDVWKKLHQQIVNVEVFNVDVVPA 240

Query: 241 EVYSSVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGS 300
           EVY++VAT VVWSMRLAL V+LY+WID++TRPIYAKLIPCDLG P K  R PLKR+ALGS
Sbjct: 241 EVYTTVATFVVWSMRLALFVSLYVWIDSITRPIYAKLIPCDLGTPTKKIRQPLKRQALGS 300

Query: 301 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHG 360
           LGKSRAKFISAEE TGVTF DFAGQEYIKRELQEIVRILKNDE+FQ+KGIYCPKGVLLHG
Sbjct: 301 LGKSRAKFISAEEKTGVTFDDFAGQEYIKRELQEIVRILKNDEEFQNKGIYCPKGVLLHG 360

Query: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 420
           PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFAS+RSYAPSIIFIDE
Sbjct: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASSRSYAPSIIFIDE 420

Query: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 480
           IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKV+T+QVLVIGATNRLDILDPALLRK
Sbjct: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVTTSQVLVIGATNRLDILDPALLRK 480

Query: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILN 540
           GRFDKIIRVGLPSKDGRLAILKVHARNKFF+SE+ KE LLQE+AE TEDFTGAELQN+LN
Sbjct: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEDEKEELLQEVAENTEDFTGAELQNVLN 540

Query: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 600
           EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTE+PEELKLRLAYREAAVA+LAC
Sbjct: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEVPEELKLRLAYREAAVAVLAC 600

Query: 601 YFPDTHRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVD 660
           Y PD +RP  ET+I SIRSQPNM Y+ET GRVF+RK DYVNSI+RACAPRV+EEEMFG++
Sbjct: 601 YLPDQYRPISETDINSIRSQPNMRYSETSGRVFARKSDYVNSIIRACAPRVVEEEMFGIE 660

Query: 661 NLCWISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAV 720
           NLCWIS+K+TLEAS+ AEF ILQTGMTAFGKAYYRN  DLVPNL  KLEALR+EY+R+AV
Sbjct: 661 NLCWISAKSTLEASQRAEFLILQTGMTAFGKAYYRNQRDLVPNLVPKLEALRDEYMRFAV 720

Query: 721 EKCFSILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE-------- 780
           EKC SIL+EY SA+E ITD+LLE+GEI+A+EIW+I+  APR PQ  +    E        
Sbjct: 721 EKCSSILQEYQSALEEITDVLLEKGEIKADEIWNIYNTAPRIPQKPVRPVDEYGALIYAG 780

Query: 781 -----------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAS 836
                       VTF+PGN GFATFGAPRPMETQ+++D+TWKL+D+IW+K+V+EI+AEA 
Sbjct: 781 RWGIHGVSLPGRVTFSPGNIGFATFGAPRPMETQIISDDTWKLVDEIWDKKVEEIKAEAV 840

BLAST of Lsi01G011480 vs. ExPASy Swiss-Prot
Match: O19922 (ATP-dependent zinc metalloprotease FtsH OS=Cyanidium caldarium OX=2771 GN=ftsH PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 4.3e-81
Identity = 192/473 (40.59%), Postives = 274/473 (57.93%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLH 355
           + GK++A+F   E  TG+ F D AG E  K ELQEIV  LK+   F + G   PKGVLL 
Sbjct: 152 NFGKAKARF-HMEAKTGIVFEDVAGIEEAKEELQEIVAFLKDSRKFTNVGATIPKGVLLV 211

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLLAKAIAGEA  PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+FID
Sbjct: 212 GPPGTGKTLLAKAIAGEASAPFFSISGSEFVEMFVGVGASRVRDLFKKAKEKAPCIVFID 271

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +R G  IGGG  EREQ L Q+LTEMDGF   T  V+V+ ATNR+D+LD ALLR
Sbjct: 272 EIDAVGRQR-GVGIGGGNDEREQTLNQLLTEMDGFSGDTG-VIVVAATNRIDVLDSALLR 331

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ I V LP+ +GRLAILKVH++ K    + +K+ LL+ IA  T  F+GA+L N+L
Sbjct: 332 PGRFDRQIMVSLPNINGRLAILKVHSKKK----KIHKDVLLEVIARRTPGFSGADLANLL 391

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA ILT R+    I  +E+ +++ +       G E S      +K  +AY EA  A+ A
Sbjct: 392 NEAAILTVRRGKVEITMKEIEDSIDK----IIAGLEGSPLADSRIKRLIAYHEAGHAVAA 451

Query: 596 CYFPDTHRPFIETNIKSIRSQPNMC-YAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFG 655
            + P  H P  +  +   R    +  +     +    K   ++ I+ A A R +EE +FG
Sbjct: 452 TFLPH-HDPVQKVTLIPRRQAKGLTWFLPNDDQFLVSKSQILSKIIAALAGRAMEEIVFG 511

Query: 656 VDNLCWISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLV---------PNLASKLE 715
           +  +   ++    + + +A   + + GM+  G     N    V           L+ ++ 
Sbjct: 512 LPEVTIGAANDIKQVTFMARQMVTKFGMSKVGPICLENSSSEVFIGRDLMGRHELSEEMV 571

Query: 716 ALREEYLRYAVEKCF----SILREYHSAVETITDILLEEGEIRAEEIWDIFEK 755
           A  +  +R  ++ C+    +IL +    ++ + + L+E+  I A+E   I E+
Sbjct: 572 AKVDLEVRSILKDCYIQARTILSQNRKLIDRVVNELVEKETIEAKEFMRIVEE 612

BLAST of Lsi01G011480 vs. ExPASy Swiss-Prot
Match: Q55700 (ATP-dependent zinc metalloprotease FtsH 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=ftsH2 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 1.2e-78
Identity = 198/513 (38.60%), Postives = 279/513 (54.39%), Query Frame = 0

Query: 261 WIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKSRAKFISAEETTGVTFSDFAG 320
           ++ NL  P+   LI     + R+ + +P       + GKS+A+F   +  TGV F D AG
Sbjct: 120 FVGNLIFPVL--LIASLFFLFRRSSNMPGGPGQAMNFGKSKARF-QMDAKTGVMFDDVAG 179

Query: 321 QEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGTGKTLLAKAIAGEAGLPFFAA 380
            +  K ELQE+V  LK  E F   G   PKGVLL GPPGTGKTLLAKAIAGEAG+PFF+ 
Sbjct: 180 IDEAKEELQEVVTFLKQPERFTAVGAKIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSI 239

Query: 381 NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAIGSKRGGPDIGGGGAEREQGL 440
           +G++FVEMFVGV ASRV+DLF  A+  AP +IFIDEIDA+G +RG   IGGG  EREQ L
Sbjct: 240 SGSEFVEMFVGVGASRVRDLFKKAKENAPCLIFIDEIDAVGRQRGA-GIGGGNDEREQTL 299

Query: 441 LQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFDKIIRVGLPSKDGRLAILKVH 500
            Q+LTEMDGF+ +T  +++I ATNR D+LD AL+R GRFD+ + V  P   GR  IL+VH
Sbjct: 300 NQLLTEMDGFEGNTG-IIIIAATNRPDVLDSALMRPGRFDRQVMVDAPDYSGRKEILEVH 359

Query: 501 ARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGILTARKDLDYIGREELLEALK 560
           ARNK    E +    +  IA  T  F+GA+L N+LNEA ILTAR+    I   E+ +A+ 
Sbjct: 360 ARNKKLAPEVS----IDSIARRTPGFSGADLANLLNEAAILTARRRKSAITLLEIDDAVD 419

Query: 561 RQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPDTHRPFIE-TNIKSIRSQPNM 620
           R       G E +  +  + K  +AY E   AI+     D H P  + T I   ++Q   
Sbjct: 420 R----VVAGMEGTPLVDSKSKRLIAYHEVGHAIVGTLLKD-HDPVQKVTLIPRGQAQGLT 479

Query: 621 CYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCWISSKATLEASKLAEFFILQ 680
            +     +  + K   +  I  A   R  EEE+FG D +   +     + +++A   + +
Sbjct: 480 WFTPNEEQGLTTKAQLMARIAGAMGGRAAEEEVFGDDEVTTGAGGDLQQVTEMARQMVTR 539

Query: 681 TGMTAFGKAYYRNPGDLV-------------PNLASKLEALREEYLRYAVEKCFSILREY 740
            GM+  G     + G  V               +A++++A   +      +    I++E 
Sbjct: 540 FGMSNLGPISLESSGGEVFLGGGLMNRSEYSEEVATRIDAQVRQLAEQGHQMARKIVQEQ 599

Query: 741 HSAVETITDILLEEGEIRAEEIWDIFEKAPRFP 760
              V+ + D+L+E+  I  EE   I  +    P
Sbjct: 600 REVVDRLVDLLIEKETIDGEEFRQIVAEYAEVP 618

BLAST of Lsi01G011480 vs. ExPASy Swiss-Prot
Match: Q10ZF7 (ATP-dependent zinc metalloprotease FtsH OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=ftsH PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.5e-78
Identity = 198/484 (40.91%), Postives = 268/484 (55.37%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLH 355
           + GKSRA+F   E  TGV F D AG E  K ELQE+V  LK  E F   G   PKGVLL 
Sbjct: 184 NFGKSRARF-QMEAKTGVLFEDVAGIEEAKEELQEVVSFLKKPEKFTAIGAKIPKGVLLV 243

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLLAKAIAGEAG+PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP IIFID
Sbjct: 244 GPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIIFID 303

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +RG   IGGG  EREQ L Q+LTEMDGF+   + +++I ATNR D+LD ALLR
Sbjct: 304 EIDAVGRQRGA-GIGGGNDEREQTLNQLLTEMDGFE-GNSGIIIIAATNRPDVLDVALLR 363

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ + V LP+  GRL IL+VHARNK    E + EA    IA  T  F+GA+L N+L
Sbjct: 364 PGRFDRQVTVDLPAYKGRLGILEVHARNKKLTPEISLEA----IARKTPGFSGADLANML 423

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA ILTAR+  + I   E+ +A+ R       G   +  +  + K  +AY E   A+L 
Sbjct: 424 NEAAILTARRRKEGITPNEIDDAIDR----VTIGLSLTPLLDGKKKRLIAYHELGHALLM 483

Query: 596 CYFPDTHRPFIETNIKSIR-----SQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEE 655
               ++      T I         +QP M        +++R    ++ I  +   R  EE
Sbjct: 484 TLLKNSDLLNKVTIIPRSGGVGGFAQPIMDEGMIDSGMYTRGW-LIDRITISLGGRAAEE 543

Query: 656 EMFGVDNLCWISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLV------------- 715
           E+FG+  +   ++      + LA   + + GM+  G     NP   V             
Sbjct: 544 EIFGLAEVTVGAANDIRSVASLAREMVTRYGMSDLGPLALENPNGEVFLGRGWQSQQPEY 603

Query: 716 -PNLASKLEALREEYLRYAVEKCFSILREYHSAVETITDILLEEGEIRAEEIWDIFEKAP 761
              +A K++      + +  EK   I+RE    ++ + D+L+E+  I  +E   I  +  
Sbjct: 604 SEEVAIKIDHQIRTMVFHCYEKARKIIRENRVLMDRLVDLLIEKETIEGDEFRRIVSEYT 655

BLAST of Lsi01G011480 vs. ExPASy Swiss-Prot
Match: Q9TJ83 (ATP-dependent zinc metalloprotease FtsH OS=Cyanidioschyzon merolae (strain 10D) OX=280699 GN=ftsH PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 4.4e-78
Identity = 185/455 (40.66%), Postives = 259/455 (56.92%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLH 355
           + GKS+A+F    + TG+ F D AG E  K EL E+V  LKN   F   G   PKGVLL 
Sbjct: 135 NFGKSKARFQMVAK-TGIMFDDVAGIEEAKEELAEVVAFLKNPSKFLAVGASIPKGVLLV 194

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLLAKAIAGEA +PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP ++FID
Sbjct: 195 GPPGTGKTLLAKAIAGEASVPFFSISGSEFVEMFVGVGASRVRDLFKKAKQNAPCLVFID 254

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +RG   IGGG  EREQ L Q+LTEMDGF+ +T  V+VI ATNR+D+LD ALLR
Sbjct: 255 EIDAVGRQRGA-GIGGGNDEREQTLNQLLTEMDGFEGNTG-VIVIAATNRVDVLDAALLR 314

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ I V +P    R+AILKVHA  K    + + EA    +A  T  F GA+L N+L
Sbjct: 315 PGRFDRQIMVSMPDVKSRIAILKVHANQKKLHPQVSLEA----VARRTAGFAGADLANLL 374

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA IL  R+ L  I  +E+ +A+ R       G E +  +  ++K  +AY E   A+ A
Sbjct: 375 NEAAILAVRRGLKQITWKEIDDAIDR----VIAGMEGTPIMDGKIKRLIAYHETGHALTA 434

Query: 596 CYFPDTHRPFIETNIKSIRSQPNMC-YAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFG 655
              P+ H P  +  +   R    +  + +   R    K   ++ I+ A   R  EE +FG
Sbjct: 435 TLLPN-HPPVQKVTLIPRRQAKGLTWFMQDNERDLLSKSQLMSMIMVALGGRAAEEAVFG 494

Query: 656 VDNLCWISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGD---------LVPNLASKLE 715
              +   +S    + + LA   + + GM++ G        +         L+P ++ ++ 
Sbjct: 495 NAEVTTGASNDLQQVTNLARQMVTRFGMSSLGPLCLETGNEEIFLGRDMRLMPEVSEEVI 554

Query: 716 ALREEYLRYAVEKCFSILREYHSAVETITDILLEE 741
           A  +  +R  +E C+  + E   A   + D ++EE
Sbjct: 555 AQIDAQVRGMIEACYEKVLELMQANRVVMDRIVEE 577

BLAST of Lsi01G011480 vs. ExPASy TrEMBL
Match: A0A6J1BUB8 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005436 PE=4 SV=1)

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 786/857 (91.72%), Postives = 804/857 (93.82%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M FMASNA NF QLSHP+LPSTTS+GRIQLVSSCSSS SNHGSA K R+R+R F+F K R
Sbjct: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAVVAS NVE D ESAQLFEKLK AERQRINKLEELQRKANLQLER
Sbjct: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD T G+A++EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDE+FQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIET+IKSIRSQPNM YAETPGRVF+RKIDYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATLEASKLAEF ILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           SILREYHSAVETITDILLE+GEIRAEEIW I+EKAPRFPQ  +    E            
Sbjct: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIR--AEASAE 837
                   VTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIR  AEASAE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840

BLAST of Lsi01G011480 vs. ExPASy TrEMBL
Match: A0A1S4DU96 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485274 PE=4 SV=1)

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 775/855 (90.64%), Postives = 801/855 (93.68%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           MKFMASNALN +QLSHPILPSTTSLGRIQL+ SCSSS SNHGSATKTRSR RS TF KFR
Sbjct: 1   MKFMASNALNSIQLSHPILPSTTSLGRIQLLPSCSSSCSNHGSATKTRSRHRSITFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAVVAS NVE DDESAQLFEKLKDAERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVVASANVEEDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLV+ASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQF+EYSNYGQTMSV
Sbjct: 121 QLVLASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFVEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDETGGSAK+EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETGGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVP+ +  LP+KR  LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPKTLPNLPIKRSLLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGV+FSDFAGQEYIKRELQEIV+ILKNDEDFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVSFSDFAGQEYIKRELQEIVQILKNDEDFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEENKEALL+EIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEENKEALLEEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKD DYI REELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILACY PD
Sbjct: 541 LTARKDQDYIEREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILACYLPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THR FIETNIKSIRS+PNM Y ETPGRVFSRKIDYVNSIVRACAPRVIEEEMFG+DNLCW
Sbjct: 601 THRSFIETNIKSIRSRPNMHYDETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGIDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKAT EA+KLA+  ILQTGMTAFGKAYYR+PGDLVPNLA KLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATTEAAKLADLLILQTGMTAFGKAYYRHPGDLVPNLAPKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           SILREYHSAVETITDILLE+  I+A EIWDIFEKAPR PQ  +    E            
Sbjct: 721 SILREYHSAVETITDILLEKEVIKAAEIWDIFEKAPRLPQPSVRPIDEHGALLYAGRWGV 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGNAG+ATFGAPRPMETQVVNDETWKLID+IW+KRVQEIRA+AS EVE
Sbjct: 781 YGVTLPGRVTFAPGNAGYATFGAPRPMETQVVNDETWKLIDNIWDKRVQEIRAKASEEVE 840

BLAST of Lsi01G011480 vs. ExPASy TrEMBL
Match: A0A6J1IPV0 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111477742 PE=4 SV=1)

HSP 1 Score: 1500.0 bits (3882), Expect = 0.0e+00
Identity = 775/855 (90.64%), Postives = 799/855 (93.45%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M FMASNALN +QLSHP LPSTTSLGRIQLVSSCSSS SNHGS TKTRSRDR FTF KFR
Sbjct: 1   MMFMASNALNLMQLSHPALPSTTSLGRIQLVSSCSSSCSNHGSPTKTRSRDRIFTFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAV+AS NVE D ESAQLFEKLKD ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNS+NVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSNNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD TGG+AK+E+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFI+T+IKSIRSQPNM YAE PGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIKTDIKSIRSQPNMHYAEIPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATL+ASKLAEF ILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           S+LREYHSAVETITD+LLE+GEIRAEEI DI+EKAPRFPQ  +    E            
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPAVRPIDEHGALMYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGN GF  FGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASA+V 
Sbjct: 781 YGVTLPGRVTFAPGNVGFVNFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASADVV 840

BLAST of Lsi01G011480 vs. ExPASy TrEMBL
Match: A0A6J1GEJ5 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111453429 PE=4 SV=1)

HSP 1 Score: 1500.0 bits (3882), Expect = 0.0e+00
Identity = 774/855 (90.53%), Postives = 800/855 (93.57%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M FMASNALN +QLSHP+LPSTTSLGRIQLVSSCSSS +NHGS TKTRSRDR FTF KFR
Sbjct: 1   MMFMASNALNLMQLSHPVLPSTTSLGRIQLVSSCSSSCANHGSPTKTRSRDRIFTFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAV+AS NVE D ESAQLFEKLKD ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDF TLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFLTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD TGG+AK+E+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLD+IGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDHIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIET+IKSIRSQPNM YAE PGRVFSRKIDY+NSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIETDIKSIRSQPNMRYAEIPGRVFSRKIDYINSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATL+ASKLAEF ILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           S+LREYHSAVETITD+LLE+GEIRAEEI DI+EKAPRFPQ  +    E            
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPTVRPIDEHGALMYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGN GF TFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAE SA+VE
Sbjct: 781 YGVTLPGRVTFAPGNVGFVTFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEVSADVE 840

BLAST of Lsi01G011480 vs. ExPASy TrEMBL
Match: A0A1S4DTH8 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485274 PE=4 SV=1)

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 712/760 (93.68%), Postives = 733/760 (96.45%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           MKFMASNALN +QLSHPILPSTTSLGRIQL+ SCSSS SNHGSATKTRSR RS TF KFR
Sbjct: 1   MKFMASNALNSIQLSHPILPSTTSLGRIQLLPSCSSSCSNHGSATKTRSRHRSITFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAVVAS NVE DDESAQLFEKLKDAERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVVASANVEEDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLV+ASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQF+EYSNYGQTMSV
Sbjct: 121 QLVLASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFVEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDETGGSAK+EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETGGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVP+ +  LP+KR  LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPKTLPNLPIKRSLLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGV+FSDFAGQEYIKRELQEIV+ILKNDEDFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVSFSDFAGQEYIKRELQEIVQILKNDEDFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEENKEALL+EIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEENKEALLEEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKD DYI REELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILACY PD
Sbjct: 541 LTARKDQDYIEREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILACYLPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THR FIETNIKSIRS+PNM Y ETPGRVFSRKIDYVNSIVRACAPRVIEEEMFG+DNLCW
Sbjct: 601 THRSFIETNIKSIRSRPNMHYDETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGIDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKAT EA+KLA+  ILQTGMTAFGKAYYR+PGDLVPNLA KLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATTEAAKLADLLILQTGMTAFGKAYYRHPGDLVPNLAPKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQ 761
           SILREYHSAVETITDILLE+  I+A EIWDIFEKAPR PQ
Sbjct: 721 SILREYHSAVETITDILLEKEVIKAAEIWDIFEKAPRLPQ 760

BLAST of Lsi01G011480 vs. NCBI nr
Match: XP_038881869.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida] >XP_038881871.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida] >XP_038881872.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1561.2 bits (4041), Expect = 0.0e+00
Identity = 802/855 (93.80%), Postives = 817/855 (95.56%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           MKFMASNALNF+Q SHPILPSTTSLGRIQLV SCSSS SNH SATKTRSRDR+FTFHKFR
Sbjct: 1   MKFMASNALNFMQFSHPILPSTTSLGRIQLVPSCSSSCSNHSSATKTRSRDRNFTFHKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKAS+SDTD VVASPNVE D ESAQLFEKLKDAERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASNSDTDLVVASPNVEEDVESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDET GSAK+EIIFRRHVIDRMPIDCWNDVWKKLHQQ+VNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETSGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQVVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNLTRP YAKLIPCDLGVP+KITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLTRPTYAKLIPCDLGVPKKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQD+GIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDRGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQ+GTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQRGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETNIKSIRSQPNM YAETPGRVFSRK+DYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETNIKSIRSQPNMRYAETPGRVFSRKLDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATLEASKLAEF ILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           S+LREYHSAVETITDILLEEGEI+AEEIWDIFEK PRFPQ  +    E            
Sbjct: 721 SVLREYHSAVETITDILLEEGEIQAEEIWDIFEKRPRFPQPSVRPIDEHGALLYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEI+AEASAEVE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIKAEASAEVE 840

BLAST of Lsi01G011480 vs. NCBI nr
Match: XP_004148651.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucumis sativus] >XP_011657750.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucumis sativus] >XP_011657752.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucumis sativus] >XP_011657753.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucumis sativus] >KAE8647463.1 hypothetical protein Csa_003652 [Cucumis sativus])

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 777/855 (90.88%), Postives = 800/855 (93.57%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           MKFMASNALNF+QLSHPILPSTTSLGRIQL+  CSSS SNHGSATKTRSRDRS TF  FR
Sbjct: 1   MKFMASNALNFIQLSHPILPSTTSLGRIQLLPPCSSSCSNHGSATKTRSRDRSITFPMFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           +HRVFTPKASSSDTDAVVASPNVE DDESAQLFEK+KDAERQRINKLEELQRKANLQLER
Sbjct: 61  KHRVFTPKASSSDTDAVVASPNVEEDDESAQLFEKVKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLT RGKLKGTEWDPENSH+INFSDF  LLNSSNVQF+EYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTKRGKLKGTEWDPENSHKINFSDFLALLNSSNVQFVEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDETGGSAK EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAE+YS
Sbjct: 181 ILPYYKDETGGSAKKEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEIYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVP+  T  PLKR ALGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPKATTNPPLKRHALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGV+F+DFAGQ+YIK ELQEIVRIL+NDEDFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVSFNDFAGQDYIKGELQEIVRILRNDEDFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAA+GTDFVEMFVGVAASRVKDLFA+ARS+APSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAASGTDFVEMFVGVAASRVKDLFANARSFAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNK F SEENKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKLFSSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKD+DYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACY PD
Sbjct: 541 LTARKDMDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYLPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETNIKSIRSQPNM YAETPGRVFSRKIDYVNSIVR CAPRVIEEEMFG+DNLCW
Sbjct: 601 THRPFIETNIKSIRSQPNMHYAETPGRVFSRKIDYVNSIVRTCAPRVIEEEMFGIDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATLEASKLAE  ILQTGMTAFGKAYYR  GDLVPNLASKL+ALREEYLRYAVEKCF
Sbjct: 661 ISSKATLEASKLAELLILQTGMTAFGKAYYRKLGDLVPNLASKLDALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           SILREYHSAVETITDILLE+GEI+AEEIWDIFEKAPRFPQ  +    E            
Sbjct: 721 SILREYHSAVETITDILLEKGEIQAEEIWDIFEKAPRFPQPSVRPIDEHGALLYAGRWGV 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIW+KRVQE+R E S EVE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWDKRVQEMRTEVSEEVE 840

BLAST of Lsi01G011480 vs. NCBI nr
Match: XP_022132617.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Momordica charantia])

HSP 1 Score: 1509.6 bits (3907), Expect = 0.0e+00
Identity = 786/857 (91.72%), Postives = 804/857 (93.82%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M FMASNA NF QLSHP+LPSTTS+GRIQLVSSCSSS SNHGSA K R+R+R F+F K R
Sbjct: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAVVAS NVE D ESAQLFEKLK AERQRINKLEELQRKANLQLER
Sbjct: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD T G+A++EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDE+FQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIET+IKSIRSQPNM YAETPGRVF+RKIDYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATLEASKLAEF ILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           SILREYHSAVETITDILLE+GEIRAEEIW I+EKAPRFPQ  +    E            
Sbjct: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIR--AEASAE 837
                   VTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIR  AEASAE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840

BLAST of Lsi01G011480 vs. NCBI nr
Match: XP_016899295.1 (PREDICTED: probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 775/855 (90.64%), Postives = 801/855 (93.68%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           MKFMASNALN +QLSHPILPSTTSLGRIQL+ SCSSS SNHGSATKTRSR RS TF KFR
Sbjct: 1   MKFMASNALNSIQLSHPILPSTTSLGRIQLLPSCSSSCSNHGSATKTRSRHRSITFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAVVAS NVE DDESAQLFEKLKDAERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVVASANVEEDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLV+ASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNSSNVQF+EYSNYGQTMSV
Sbjct: 121 QLVLASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFVEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDETGGSAK+EIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETGGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVP+ +  LP+KR  LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPKTLPNLPIKRSLLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGV+FSDFAGQEYIKRELQEIV+ILKNDEDFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVSFSDFAGQEYIKRELQEIVQILKNDEDFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEENKEALL+EIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEENKEALLEEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKD DYI REELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILACY PD
Sbjct: 541 LTARKDQDYIEREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILACYLPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THR FIETNIKSIRS+PNM Y ETPGRVFSRKIDYVNSIVRACAPRVIEEEMFG+DNLCW
Sbjct: 601 THRSFIETNIKSIRSRPNMHYDETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGIDNLCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKAT EA+KLA+  ILQTGMTAFGKAYYR+PGDLVPNLA KLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATTEAAKLADLLILQTGMTAFGKAYYRHPGDLVPNLAPKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           SILREYHSAVETITDILLE+  I+A EIWDIFEKAPR PQ  +    E            
Sbjct: 721 SILREYHSAVETITDILLEKEVIKAAEIWDIFEKAPRLPQPSVRPIDEHGALLYAGRWGV 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGNAG+ATFGAPRPMETQVVNDETWKLID+IW+KRVQEIRA+AS EVE
Sbjct: 781 YGVTLPGRVTFAPGNAGYATFGAPRPMETQVVNDETWKLIDNIWDKRVQEIRAKASEEVE 840

BLAST of Lsi01G011480 vs. NCBI nr
Match: XP_022977393.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977394.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977395.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977396.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1500.0 bits (3882), Expect = 0.0e+00
Identity = 775/855 (90.64%), Postives = 799/855 (93.45%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M FMASNALN +QLSHP LPSTTSLGRIQLVSSCSSS SNHGS TKTRSRDR FTF KFR
Sbjct: 1   MMFMASNALNLMQLSHPALPSTTSLGRIQLVSSCSSSCSNHGSPTKTRSRDRIFTFPKFR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           QHRVFTPKASSSDTDAV+AS NVE D ESAQLFEKLKD ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNS+NVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSNNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDETGGSAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD TGG+AK+E+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFI+T+IKSIRSQPNM YAE PGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIKTDIKSIRSQPNMHYAEIPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720
           ISSKATL+ASKLAEF ILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKCF
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE------------ 780
           S+LREYHSAVETITD+LLE+GEIRAEEI DI+EKAPRFPQ  +    E            
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPAVRPIDEHGALMYAGRWGI 780

Query: 781 -------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASAEVE 837
                   VTFAPGN GF  FGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASA+V 
Sbjct: 781 YGVTLPGRVTFAPGNVGFVNFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEASADVV 840

BLAST of Lsi01G011480 vs. TAIR 10
Match: AT5G64580.1 (AAA-type ATPase family protein )

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 636/858 (74.13%), Postives = 726/858 (84.62%), Query Frame = 0

Query: 1   MKFMASNALNFVQLSHPILPSTTSLGRIQLVSSCSSSRSNHGSATKTRSRDRSFTFHKFR 60
           M F  S++L     S P+ PS T        S  S  R    +  K  S+   F     R
Sbjct: 1   MTFYISSSLTPTHFSKPLNPSNTLFPSQFRGSLSSFVRRRKPTEAKLSSK---FNLFPSR 60

Query: 61  QHRVFTPKASSSDTDAVVASPNVEGDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120
           ++ + T   S+S  ++  +S + E D ES +LFEKL++ ER+R++ +EEL+RKAN+QLER
Sbjct: 61  RNGLIT-CCSTSSFESTESSVSQEEDAESNRLFEKLRETERERLSNMEELERKANVQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMAS WSR LLTMRGKLKGTEWDPE SHRINFSDF  LL+S++VQ++EYSNYGQT+SV
Sbjct: 121 QLVMASDWSRTLLTMRGKLKGTEWDPETSHRINFSDFMKLLDSNSVQYMEYSNYGQTISV 180

Query: 181 ILPYYKD-ETGG---SAKSEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPA 240
           ILPYYKD E  G    +K EIIFRRH++DRMPID WNDVWKKLHQQIVNV+V NVD VPA
Sbjct: 181 ILPYYKDGEPLGEEEDSKKEIIFRRHIVDRMPIDGWNDVWKKLHQQIVNVEVFNVDVVPA 240

Query: 241 EVYSSVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPRKITRLPLKRRALGS 300
           EVY++VAT VVWSMRLAL V+LY+WID++TRPIYAKLIPCDLG P K  R PLKR+ALGS
Sbjct: 241 EVYTTVATFVVWSMRLALFVSLYVWIDSITRPIYAKLIPCDLGTPTKKIRQPLKRQALGS 300

Query: 301 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHG 360
           LGKSRAKFISAEE TGVTF DFAGQEYIKRELQEIVRILKNDE+FQ+KGIYCPKGVLLHG
Sbjct: 301 LGKSRAKFISAEEKTGVTFDDFAGQEYIKRELQEIVRILKNDEEFQNKGIYCPKGVLLHG 360

Query: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 420
           PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFAS+RSYAPSIIFIDE
Sbjct: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASSRSYAPSIIFIDE 420

Query: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 480
           IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKV+T+QVLVIGATNRLDILDPALLRK
Sbjct: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVTTSQVLVIGATNRLDILDPALLRK 480

Query: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILN 540
           GRFDKIIRVGLPSKDGRLAILKVHARNKFF+SE+ KE LLQE+AE TEDFTGAELQN+LN
Sbjct: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEDEKEELLQEVAENTEDFTGAELQNVLN 540

Query: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 600
           EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTE+PEELKLRLAYREAAVA+LAC
Sbjct: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEVPEELKLRLAYREAAVAVLAC 600

Query: 601 YFPDTHRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVD 660
           Y PD +RP  ET+I SIRSQPNM Y+ET GRVF+RK DYVNSI+RACAPRV+EEEMFG++
Sbjct: 601 YLPDQYRPISETDINSIRSQPNMRYSETSGRVFARKSDYVNSIIRACAPRVVEEEMFGIE 660

Query: 661 NLCWISSKATLEASKLAEFFILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAV 720
           NLCWIS+K+TLEAS+ AEF ILQTGMTAFGKAYYRN  DLVPNL  KLEALR+EY+R+AV
Sbjct: 661 NLCWISAKSTLEASQRAEFLILQTGMTAFGKAYYRNQRDLVPNLVPKLEALRDEYMRFAV 720

Query: 721 EKCFSILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFPQVVLFDFRE-------- 780
           EKC SIL+EY SA+E ITD+LLE+GEI+A+EIW+I+  APR PQ  +    E        
Sbjct: 721 EKCSSILQEYQSALEEITDVLLEKGEIKADEIWNIYNTAPRIPQKPVRPVDEYGALIYAG 780

Query: 781 -----------MVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAS 836
                       VTF+PGN GFATFGAPRPMETQ+++D+TWKL+D+IW+K+V+EI+AEA 
Sbjct: 781 RWGIHGVSLPGRVTFSPGNIGFATFGAPRPMETQIISDDTWKLVDEIWDKKVEEIKAEAV 840

BLAST of Lsi01G011480 vs. TAIR 10
Match: AT1G50250.1 (FTSH protease 1 )

HSP 1 Score: 289.3 bits (739), Expect = 1.0e-77
Identity = 185/475 (38.95%), Postives = 270/475 (56.84%), Query Frame = 0

Query: 298 GKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGP 357
           G+S++KF    E TGV+F+D AG +  K ELQE+V  LKN + +   G   PKG LL GP
Sbjct: 245 GRSKSKFQEVPE-TGVSFADVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGP 304

Query: 358 PGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEI 417
           PGTGKTLLA+A+AGEAG+PFF+   ++FVE+FVGV ASRV+DLF  A+S AP I+FIDEI
Sbjct: 305 PGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEI 364

Query: 418 DAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKG 477
           DA+G +RG   +GGG  EREQ + Q+LTEMDGF    + V+V+ ATNR D+LD ALLR G
Sbjct: 365 DAVGRQRGA-GMGGGNDEREQTINQLLTEMDGFS-GNSGVIVLAATNRPDVLDSALLRPG 424

Query: 478 RFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNE 537
           RFD+ + V  P   GR+ IL+VH+R K       K+    ++A  T  FTGA+LQN++NE
Sbjct: 425 RFDRQVTVDRPDVAGRVKILQVHSRGKAL----GKDVDFDKVARRTPGFTGADLQNLMNE 484

Query: 538 AGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACY 597
           A IL AR++L  I ++E+ +AL+R        ++ +  + EE K  +AY EA  A++   
Sbjct: 485 AAILAARRELKEISKDEISDALER---IIAGPEKKNAVVSEEKKRLVAYHEAGHALVGAL 544

Query: 598 FPDTHRPFIETNIKSIRSQPNMCYAETPGR------VFSRKIDYVNSIVRACAPRVIEEE 657
            P+ + P  + +I   R Q        P        ++SR     N +  A   RV EE 
Sbjct: 545 MPE-YDPVAKISIIP-RGQAGGLTFFAPSEERLESGLYSRSY-LENQMAVALGGRVAEEV 604

Query: 658 MFGVDNLCWISSKATLEASKLAEFFILQTGMT-AFGKAYYRNPG-------------DLV 717
           +FG +N+   +S   ++ S++A   I + G +   G+     PG             D  
Sbjct: 605 IFGDENVTTGASNDFMQVSRVARQMIERFGFSKKIGQVAVGGPGGNPFMGQQMSSQKDYS 664

Query: 718 PNLASKLEALREEYLRYAVEKCFSILREYHSAVETITDILLEEGEIRAEEIWDIF 753
              A  ++A   E +  A ++   I+  +   +  +  +L+E+  +  EE   +F
Sbjct: 665 MATADIVDAEVRELVEKAYKRATEIITTHIDILHKLAQLLIEKETVDGEEFMSLF 706

BLAST of Lsi01G011480 vs. TAIR 10
Match: AT5G42270.1 (FtsH extracellular protease family )

HSP 1 Score: 287.0 bits (733), Expect = 5.0e-77
Identity = 187/475 (39.37%), Postives = 267/475 (56.21%), Query Frame = 0

Query: 298 GKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHGP 357
           G+S++KF    E TGVTF D AG +  K ELQE+V  LKN + +   G   PKG LL GP
Sbjct: 233 GRSKSKFQEVPE-TGVTFGDVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGP 292

Query: 358 PGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEI 417
           PGTGKTLLA+A+AGEAG+PFF+   ++FVE+FVGV ASRV+DLF  A+S AP I+FIDEI
Sbjct: 293 PGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEI 352

Query: 418 DAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKG 477
           DA+G +RG   +GGG  EREQ + Q+LTEMDGF    + V+V+ ATNR D+LD ALLR G
Sbjct: 353 DAVGRQRGA-GMGGGNDEREQTINQLLTEMDGFS-GNSGVIVLAATNRPDVLDSALLRPG 412

Query: 478 RFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNE 537
           RFD+ + V  P   GR+ ILKVH+R K       K+   +++A  T  FTGA+LQN++NE
Sbjct: 413 RFDRQVTVDRPDVAGRVQILKVHSRGKAI----GKDVDYEKVARRTPGFTGADLQNLMNE 472

Query: 538 AGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACY 597
           A IL AR++L  I ++E+ +AL+R        ++ +  + EE K  +AY EA  A++   
Sbjct: 473 AAILAARRELKEISKDEISDALER---IIAGPEKKNAVVSEEKKRLVAYHEAGHALVGAL 532

Query: 598 FPDTHRPFIETNIKSIRSQPNMCYAETPGR------VFSRKIDYVNSIVRACAPRVIEEE 657
            P+ + P  + +I   R Q        P        ++SR     N +  A   RV EE 
Sbjct: 533 MPE-YDPVAKISIIP-RGQAGGLTFFAPSEERLESGLYSRSY-LENQMAVALGGRVAEEV 592

Query: 658 MFGVDNLCWISSKATLEASKLAE------FFILQTGMTAFGKA--------YYRNPGDLV 717
           +FG +N+   +S   ++ S++A        F  + G  A G A           +  D  
Sbjct: 593 IFGDENVTTGASNDFMQVSRVARQMVERFGFSKKIGQVAVGGAGGNPFLGQSMSSQKDYS 652

Query: 718 PNLASKLEALREEYLRYAVEKCFSILREYHSAVETITDILLEEGEIRAEEIWDIF 753
              A  ++A   E +  A  +   I+      +  +  +L+E+  +  EE   +F
Sbjct: 653 MATADVVDAEVRELVEKAYVRAKEIITTQIDILHKLAQLLIEKETVDGEEFMSLF 694

BLAST of Lsi01G011480 vs. TAIR 10
Match: AT5G15250.1 (FTSH protease 6 )

HSP 1 Score: 283.1 bits (723), Expect = 7.2e-76
Identity = 204/555 (36.76%), Postives = 289/555 (52.07%), Query Frame = 0

Query: 209 IDCWNDVWKKLHQQIVNVDVINVDAVPAEVYSSVATAVVWSMRLALSVALYLWIDNLTRP 268
           ++  N V  K+ +  VN+  + VD V  E+           M +     L  ++ NL  P
Sbjct: 122 VEISNPVVGKIQRVRVNLPGLPVDLV-REMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFP 181

Query: 269 IYAKLIPCDLGVPRKITRLPLKRRALGSLGKSRAKFISAEETTGVTFSDFAGQEYIKREL 328
           +   ++   L +     R P        LG+S+AKF   E  TG+TF D AG +  K++ 
Sbjct: 182 L---ILLVSLLLTSSSRRNPAGPNLPFGLGRSKAKF-QMEPNTGITFEDVAGVDEAKQDF 241

Query: 329 QEIVRILKNDEDFQDKGIYCPKGVLLHGPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEM 388
           +EIV  LK  E F   G   PKGVLL GPPGTGKTLLAKAIAGEAG+PFF+ +G++F+EM
Sbjct: 242 EEIVEFLKTPEKFSALGAKIPKGVLLTGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFIEM 301

Query: 389 FVGVAASRVKDLFASARSYAPSIIFIDEIDAIGSKRGGPDIGGGGAEREQGLLQILTEMD 448
           FVGV ASR +DLF  A++ +P I+FIDEIDA+G  R G  IGGG  EREQ L QILTEMD
Sbjct: 302 FVGVGASRARDLFNKAKANSPCIVFIDEIDAVGRMR-GTGIGGGNDEREQTLNQILTEMD 361

Query: 449 GFKVSTAQVLVIGATNRLDILDPALLRKGRFDKIIRVGLPSKDGRLAILKVHARNKFFQS 508
           GF  +T  V+VI ATNR +ILD ALLR GRFD+ + VGLP   GR  ILKVH+R+K    
Sbjct: 362 GFAGNTG-VIVIAATNRPEILDSALLRPGRFDRQVSVGLPDIRGREEILKVHSRSK---- 421

Query: 509 EENKEALLQEIAELTEDFTGAELQNILNEAGILTARKDLDYIGREELLEALKRQKGTFET 568
           + +K+  L  IA  T  F+GA+L N++NEA IL  R+  D I   E+ +++ R       
Sbjct: 422 KLDKDVSLSVIAMRTPGFSGADLANLMNEAAILAGRRGKDKITLTEIDDSIDR----IVA 481

Query: 569 GQEDSTEIPEELKLRLAYREAAVAILACYFPDTHRPFIETNIKSIRSQPNMCYAETPGR- 628
           G E +  I  + K  +AY E   AI A    + H P  +  +   R Q        PG  
Sbjct: 482 GMEGTKMIDGKSKAIVAYHEVGHAICAT-LTEGHDPVQKVTLVP-RGQARGLTWFLPGED 541

Query: 629 -VFSRKIDYVNSIVRACAPRVIEEEMFGVDNLCWISSKATLEASKLAEFFILQTGMTAFG 688
                K      IV     R  E+ +FG   +   ++    + +++A   +   GM+  G
Sbjct: 542 PTLVSKQQLFARIVGGLGGRAAEDVIFGEPEITTGAAGDLQQVTEIARQMVTMFGMSEIG 601

Query: 689 KAYYRNP--------------GDLVPNLASKLEALREEYLRYAVEKCFSILREYHSAVET 748
                +P                +   LA  +++  ++ +  A E     +R    A++ 
Sbjct: 602 PWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKKHVRNNREAIDK 659

BLAST of Lsi01G011480 vs. TAIR 10
Match: AT1G06430.1 (FTSH protease 8 )

HSP 1 Score: 282.0 bits (720), Expect = 1.6e-75
Identity = 188/478 (39.33%), Postives = 257/478 (53.77%), Query Frame = 0

Query: 297 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDKGIYCPKGVLLHG 356
           +G+S+AKF   E  TGVTF D AG +  K++  E+V  LK  E F   G   PKGVLL G
Sbjct: 202 IGQSKAKF-QMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVG 261

Query: 357 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 416
           PPGTGKTLLAKAIAGEAG+PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+F+DE
Sbjct: 262 PPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDE 321

Query: 417 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 476
           IDA+G +R G  IGGG  EREQ L Q+LTEMDGF+ +T  V+V+ ATNR DILD ALLR 
Sbjct: 322 IDAVGRQR-GTGIGGGNDEREQTLNQLLTEMDGFEGNTG-VIVVAATNRADILDSALLRP 381

Query: 477 GRFDKIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILN 536
           GRFD+ + V +P   GR  ILKVH+ NK F+S  +    L+ IA  T  F+GA+L N+LN
Sbjct: 382 GRFDRQVSVDVPDVKGRTDILKVHSGNKKFESGVS----LEVIAMRTPGFSGADLANLLN 441

Query: 537 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 596
           EA IL  R+    I  +E+ +++ R       G E +     + K  +AY E   AI   
Sbjct: 442 EAAILAGRRGKTAISSKEIDDSIDR----IVAGMEGTVMTDGKSKSLVAYHEVGHAICGT 501

Query: 597 YFPDTHRPFIETNIKSIRSQPNMCYAETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVD 656
             P        T I   +++    +  +       K      IV     R  EE +FG  
Sbjct: 502 LTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEVIFGES 561

Query: 657 NLCWISSKATLEASKLAEFFILQTGMTAFG---------------KAYYRNPGDLVPNLA 716
            +   +     + + LA+  +   GM+  G               +   RN   +   LA
Sbjct: 562 EVTTGAVSDLQQITGLAKQMVTTFGMSEIGPWSLMDSSEQSDVIMRMMARN--SMSEKLA 621

Query: 717 SKLEALREEYLRYAVEKCFSILREYHSAVETITDILLEEGEIRAEEIWDIFEKAPRFP 760
           + ++   +     A E   S +R    A++ I +ILLE+  +  +E   I  +    P
Sbjct: 622 NDIDTAVKTLSDKAYEIALSQIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIP 666