Lsi01G011280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G011280
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	WAT1-related protein
Location	chr01: 9413047 .. 9416383 (-)
RNA-Seq Expression	Lsi01G011280
Synteny	Lsi01G011280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGGAAGAAGGAAGAAGGAAGAAGGAAGAAGGAAGAAGGAAAAATTAATTTGGTTTATGATAAAAGAAATGGATTCAAAGAAGCCTTACATTGCTGCTATTGTTTGTCAAATCAGTTTTGCTGGGATGAGTGTATTCTCAAAGGCTGCCTTTGCTTCTGGTATGAATACTTATATTTTCCTTTTCTACAGGCAAACTGTTGGGACTCTCTTCTTACTGCCCCCCACCATATATTTCAAAAGGTAAATCAAATTATTATTTCACCTCCATTTTATTATATACTCTTGATTTTATTGTTTATAAGTTATATCCTAGATCAAGGGCGTAAGATCTAAGTGAGAGCTGACAATCGTCTTCATTAGTCTCGTGTCTTTTTTTGACAAAACCAAAGCCAAAGGGTCACTCGAAGTGGACCATATACATATTGCACCATTGTAAGAATATATAGAGGTTCAATTGTTCTTTAAATTAAACTAGTGCTATCAAAGCTTCTTAAGTGTGGTGAAAATAGGTGAAAGCCTTGATAACATAGCCTTGATCTACTGAACACATAGCGGTATATATTTTAAACAAGTGTTTGCAGAGAATAAAAAAAAAAAAAAAAAAGTTAAATATAATTTTGGTCCCTGTACTTTAAAGTTTGTTCAATTTGAGTCCATATATTTTCAAATGTTCAATTTTAGTCATTGTACTTTCAATAAATCTTAAATCTAGTCCCTATTACTAGTTATTGTGAATTTTTTAACTAGTTTTTATTTTTTTATTATCATTTTTCAATATAAATTTTGAAAATATATTCACATATTGTGTTTCTTTCCATGAAAATTATTGTGATTATTTAACCAATTTTGATAAAAATTAACTTTGAGGGACTAAATTTAAGATTTACTGTATACATGAACTAAAATTGGACATTTAAAATTACCGGAGCTAAAATTGAACAAATTTCAAAGTACAGAAACTAAAATGGTGTTTTAATCGACTATGTGGTCCTTTGTTTCTGGGGAGAATTATTGTGGGAGTGCCACAAAAGTTTTATGTAGACTAAGTAAGAAGAATAATAGTATAATAACCGAACCATCATAGATGGGCCTAGTGGTAGTGGGAACATAAAAAAAAAAAAAAGAATACGGATTGTGCTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTACTAATCTTATGGGATATTAAATCTTAGGGGACTTTTTTTTTTTTTTTGTTAAAAAAAATCTAACCAATTTTTTTTCCTCTGTCCTTCTCCCTTTAACAAGTCTTTAAGTCTTTCTTCCTTTTTTTTTTTTGGCATGGATTTTAGAAAGGAAATAGCAGGATTATCAGTGGGAGATGTGTGGAAGATTTTCTTACAGGCCATTTTGGGGTTTGTACAATTAATTATAAATTATTTTTATTCTCTGGATTTAAGATTATTTAATGGATATTTTTGGGAATGATTTTTGTGTGTAAAAAGTGCAGGTATACACTGGGTTTGAATGCTTATGGGTTAGGAGTTAAGTATACATCAGCAACTTTGGGTGCTGCAGCCTTTAATTGTGTTCCTGTCACCACTTTCTTCTTTGCTCTTATTTCAAGGTTCGATAACATATATACTTTTTTCCCCTAAATAAATTTAACCCACACAATTTAAAACTATATATTAACAAAAGATGCACCAAAAAAAAAAAGAAAGAAAAGAAAAAAAAAAGGGCCGAGGTAACAATCCAGCACAATTTTCAAAAAATAGATAATTATTTTAGATTTTTTTACAGTATATGAGATAGGAAGATCGAAACTCAAACCTCAAAATTAATAGCACACTTGTATATATAATAATGTATATATGTGTGTTCTTGATAGGATGGAGAAAGTAAACATAAGAAAAGCAAGTGGAATGGCAAAGATTGGAGGCATAATGTTGTGCATTGCAGGCGTTGCAGTTCTTGCATTTTACAAAGGCCCTTATTTCAAACCACTCTTCAATTTTCACCTTTTTCAAATCCATCAATCTCATCTTTCTTCCCCAAAAACGTGGATTCTTGGTTGTTTTTTGCTCCTTGTTTCTTCCCTTTCATGGGGTTTGTGGTTTGTCCTTCAGGTTAATTCATTTTCAATATTACCAAACTTATCTATTACTACATTCTAGACCGTACGCATCCTTTTTGCAGCCTACTCTAACTATACAGTAGAAAAAATAAAAAAATAAAAAAATAAAAACTAATCACATGATTGGAAGAAGCTAAGCTGCAAAAATATTGGTGCTATCTCTAGATCCAAAAATATTTATCTTTCTTATATTTGGTTTGATGTAATATTTTGTTTTTCAGGCTTGGGTTTTGAAGACTTGTCCATCTCCACTTGTGTTAACGTTTGGACAAACATTTTCAAGTACCATTCAATCTTTCGTTGTGGCTATTGCAATGGAAAGAAACCCTTTTGAATGGAAGTTGGCTTGGAATATTCGTCTCATTGCTATTCTTTATTGCGTATGATAATTATCGTTGAGTTATAGTCTCTTTATCTAATTATTATTAATTTTATAAATAATTATTCTAATTAATTAAAAAAAAAAATTGTTGCAGGGAATTTTTGCAATGTGTATTGCAAACTATCTGTCAAGTTGGGTGATAAAAAAGAAGGGTCCAGTTTTCCAAGCTGTTTCCACACCCTTCAATCTCATTTTCACACTCATTGGTTCAGAATTTCTTTTAAATGATGGAGTTAGCTTGGGAAGGTAAATTGAAAATTAATTAATTGATAGATTATTCCATTAATAATTATTTAAATATTATTTTTTTTATGGTGTGTATGTTGAACAGTATAATAGGTGCCATGCTGCTGGTTTTGAGCCTCTATAGTGTTTTATGGGGAAAAAAGAAGGAAATGAGTTGCCATGATACAGAAAACAAGATTGATTCTGTTCCATTGGACAAGGAAATGTTAGATCACATTTGAATTTTGTAGGATATGTAATTCTCGATGTTCTTATCAATTGAGTTATACTTAAATAGACTATTATATATATATATATATATATATATATATTATTTTATTATTTTCTTTTTCTATAAAAAAAA

mRNA sequence

GAAGGAAGAAGGAAGAAGGAAGAAGGAAGAAGGAAGAAGGAAAAATTAATTTGGTTTATGATAAAAGAAATGGATTCAAAGAAGCCTTACATTGCTGCTATTGTTTGTCAAATCAGTTTTGCTGGGATGAGTGTATTCTCAAAGGCTGCCTTTGCTTCTGGTATGAATACTTATATTTTCCTTTTCTACAGGCAAACTGTTGGGACTCTCTTCTTACTGCCCCCCACCATATATTTCAAAAGAAAGGAAATAGCAGGATTATCAGTGGGAGATGTGTGGAAGATTTTCTTACAGGCCATTTTGGGGTATACACTGGGTTTGAATGCTTATGGGTTAGGAGTTAAGTATACATCAGCAACTTTGGGTGCTGCAGCCTTTAATTGTGTTCCTGTCACCACTTTCTTCTTTGCTCTTATTTCAAGGCGTTGCAGTTCTTGCATTTTACAAAGGCCCTTATTTCAAACCACTCTTCAATTTTCACCTTTTTCAAATCCATCAATCTCATCTTTCTTCCCCAAAAACGTGGATTCTTGGTTGTTTTTTGCTCCTTGTTTCTTCCCTTTCATGGGGTTTGTGGCTTGGGTTTTGAAGACTTGTCCATCTCCACTTGTGTTAACGTTTGGACAAACATTTTCAAGTACCATTCAATCTTTCGTTGTGGCTATTGCAATGGAAAGAAACCCTTTTGAATGGAAGTTGGCTTGGAATATTCGTCTCATTGCTATTCTTTATTGCGGAATTTTTGCAATGTGTATTGCAAACTATCTGTCAAGTTGGGTGATAAAAAAGAAGGGTCCAGTTTTCCAAGCTGTTTCCACACCCTTCAATCTCATTTTCACACTCATTGGTTCAGAATTTCTTTTAAATGATGGAGTTAGCTTGGGAAGTATAATAGGTGCCATGCTGCTGGTTTTGAGCCTCTATAGTGTTTTATGGGGAAAAAAGAAGGAAATGAGTTGCCATGATACAGAAAACAAGATTGATTCTGTTCCATTGGACAAGGAAATGTTAGATCACATTTGAATTTTGTAGGATATGTAATTCTCGATGTTCTTATCAATTGAGTTATACTTAAATAGACTATTATATATATATATATATATATATATATATTATTTTATTATTTTCTTTTTCTATAAAAAAAA

Coding sequence (CDS)

ATGATAAAAGAAATGGATTCAAAGAAGCCTTACATTGCTGCTATTGTTTGTCAAATCAGTTTTGCTGGGATGAGTGTATTCTCAAAGGCTGCCTTTGCTTCTGGTATGAATACTTATATTTTCCTTTTCTACAGGCAAACTGTTGGGACTCTCTTCTTACTGCCCCCCACCATATATTTCAAAAGAAAGGAAATAGCAGGATTATCAGTGGGAGATGTGTGGAAGATTTTCTTACAGGCCATTTTGGGGTATACACTGGGTTTGAATGCTTATGGGTTAGGAGTTAAGTATACATCAGCAACTTTGGGTGCTGCAGCCTTTAATTGTGTTCCTGTCACCACTTTCTTCTTTGCTCTTATTTCAAGGCGTTGCAGTTCTTGCATTTTACAAAGGCCCTTATTTCAAACCACTCTTCAATTTTCACCTTTTTCAAATCCATCAATCTCATCTTTCTTCCCCAAAAACGTGGATTCTTGGTTGTTTTTTGCTCCTTGTTTCTTCCCTTTCATGGGGTTTGTGGCTTGGGTTTTGAAGACTTGTCCATCTCCACTTGTGTTAACGTTTGGACAAACATTTTCAAGTACCATTCAATCTTTCGTTGTGGCTATTGCAATGGAAAGAAACCCTTTTGAATGGAAGTTGGCTTGGAATATTCGTCTCATTGCTATTCTTTATTGCGGAATTTTTGCAATGTGTATTGCAAACTATCTGTCAAGTTGGGTGATAAAAAAGAAGGGTCCAGTTTTCCAAGCTGTTTCCACACCCTTCAATCTCATTTTCACACTCATTGGTTCAGAATTTCTTTTAAATGATGGAGTTAGCTTGGGAAGTATAATAGGTGCCATGCTGCTGGTTTTGAGCCTCTATAGTGTTTTATGGGGAAAAAAGAAGGAAATGAGTTGCCATGATACAGAAAACAAGATTGATTCTGTTCCATTGGACAAGGAAATGTTAGATCACATTTGA

Protein sequence

MIKEMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRRCSSCILQRPLFQTTLQFSPFSNPSISSFFPKNVDSWLFFAPCFFPFMGFVAWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDHI

Homology

[Close]

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.7e-54
Identity = 133/354 (37.57%), Postives = 187/354 (52.82%), Query Frame = 0


Query: 4   EMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRK 63
           +M+SKKPY+   + Q+ +  M + SKA F  GMNT++F+FYRQ   T+FL P   +F+RK
Sbjct: 2   DMESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERK 61

Query: 64  EIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRR 123
               LS     KIF+ ++ G TL L+  G+ + YTSATL AA    +P  TFF AL+   
Sbjct: 62  SAPPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGM 121

Query: 124 -------------------CSSCILQRPLFQTTLQFSPF-------------SNPSISSF 183
                              C   ++   +++  L   P              +NP   S 
Sbjct: 122 ERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVS- 181

Query: 184 FPKNVDSWLFFAPCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVA 243
                 SWL    C       + W         VLK  PS L  T      S+IQSFV+A
Sbjct: 182 --GGSTSWL--KGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIA 241

Query: 244 IAMERNPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTL 303
           IA+ER+   WKL WN+RL+A++YCG     +A YL SWVI+K+GPVF ++ TP +L+FTL
Sbjct: 242 IALERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTL 301

Query: 304 IGSEFLLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKE 317
           + S  LL + +SLGSI+G +LL++ LY VLWGK +E    +  +  D + L KE
Sbjct: 302 LSSAILLCEIISLGSIVGGLLLIIGLYCVLWGKSRE----EKNSGDDKIDLQKE 346

[Close]

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 9.4e-37
Identity = 113/320 (35.31%), Postives = 163/320 (50.94%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           M   K  +A +  QI +AGM + SK A + G N ++F+FYRQ    L L P   + +  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRRC 124
            + LS   + KIF  ++ G TL LN Y + ++ T+AT  AA  N +P  TF  AL+ R  
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 125 SSCILQ-RPLFQTT---------LQFSPFSNPSI-----SSFFP-----------KNVDS 184
           +  + +   + + T         L F+    PS+     SS  P           K   +
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSLINHYNSSTIPNGTVPSTKNSVKGSIT 181

Query: 185 WLFFAPCFFPFMGFVAWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKLAWNI 244
            L    C+  ++   + V+K  P+ L L   Q   S IQS V A+A+ RNP  WK+ + +
Sbjct: 182 MLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFGL 241

Query: 245 RLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLGSI 299
            L+++ YCGI    +  +L  W I+KKGPVF A+ TP  LI T I S FL  +   LGS+
Sbjct: 242 PLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGSV 301

[Close]

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.0e-35
Identity = 108/344 (31.40%), Postives = 166/344 (48.26%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           + S KPY A I  Q  +AGM++ +K +  +GM+ Y+ + YR  + T  + P   +F+RK 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRR- 124
              ++     ++F+  +LG  +  N Y +G+KYTS T   A  N +P  TF  A++ R  
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 125 --------CSS---------------CILQRP---LFQTT---LQFSPFSNPSIS----- 184
                   C +                I + P   LF T    +Q S  +N + S     
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSS 192

Query: 185 -SFFPKNVDSWLFFAPCFFPFMGFVAWVLKT-CPSPLVLTFGQTFSSTIQSFVVAIAMER 244
              F K     +F    +       A +LKT     L LT    F  T+Q+  V   ME 
Sbjct: 193 DKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEH 252

Query: 245 NPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEF 304
           NP  W++ W++ L+A  Y GI A  I+ Y+   V+KK+GPVF    +P  ++   +   F
Sbjct: 253 NPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSF 312

Query: 305 LLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTE-NKIDS 311
           +L + + LG +IGA+L+V+ LY+VLWGK+KE      E  KIDS
Sbjct: 313 VLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDS 356

[Close]

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot
Match: Q9SUD5 (WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 9.7e-34
Identity = 96/334 (28.74%), Postives = 164/334 (49.10%), Query Frame = 0


Query: 4   EMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIY--FK 63
           E+   K  +A ++ Q + AG+++F+KAAF  G+N  +F+ YRQ + TLF+ P +    ++
Sbjct: 2   EISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWR 61

Query: 64  RKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFF----- 123
           ++    L V   W + L A++G T+  NAY  G+  +S+++  A  N +P  TF      
Sbjct: 62  KENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIV 121

Query: 124 -FALISRRCSSCILQ---------RPLFQTTLQFSPFSNPSISSFFPKNVDSWLFFAPCF 183
            F  I RR    + +           +  T L+     N  +      N D+  +   CF
Sbjct: 122 GFESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNALL------NQDNTAWLLGCF 181

Query: 184 FPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPF-EWKLAW 243
           F  +   AW         +   CP  L  +    F +TI SF+VA+A+       WKL  
Sbjct: 182 FLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPPWKLDS 241

Query: 244 NIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLG 303
            ++L   +Y G F + I+ +L +W++ +KGPVF A+  P + +         L +   LG
Sbjct: 242 FLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKEQTYLG 301

Query: 304 SIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDS 311
           S++GA+ ++L LY VLWGK ++     T+ K+++
Sbjct: 302 SLLGALAIILGLYIVLWGKSEDYQEESTDLKLEN 328

[Close]

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.2e-33
Identity = 101/328 (30.79%), Postives = 154/328 (46.95%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           M+  +P+I+ +V Q+  AGM + SKA    GM+ Y+ + YR  V T+ + P   YF +K 
Sbjct: 10  MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKV 69

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALI---S 124
              +++   +KI L  +L   +  N Y LG+KYT+AT   A +N +P  TF  A I    
Sbjct: 70  RPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLE 129

Query: 125 RRCSSCILQRPLFQTTLQ-------FSPFSNPSISSFFPKNVDSW--------------- 184
           R    CI        TL         +    P +  F+ K V +                
Sbjct: 130 RVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIHSAIKGAV 189

Query: 185 LFFAPCF--FPFMGFVAWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMER-NPFEWKLAW 244
           L    CF    FM   A  L+T P+ L LT       TI+   VA+ ME+ NP  W + W
Sbjct: 190 LVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGW 249

Query: 245 NIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLG 304
           + +L+   Y GI    +A Y+   V+K +GPVF    +P  +I   I S  +  + + LG
Sbjct: 250 DTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLG 309

[Close]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL
Match: A0A1S3B374 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485250 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 3.5e-111
Identity = 227/349 (65.04%), Postives = 263/349 (75.36%), Query Frame = 0


Query: 1   MIKEMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYF 60
           M ++  SKKPY  A+VCQI+FAGMS+FSKAAFASGMNTYIFLFYRQ +GT+ LLP TIYF
Sbjct: 1   MEEKKGSKKPYFGALVCQITFAGMSLFSKAAFASGMNTYIFLFYRQALGTIVLLPLTIYF 60

Query: 61  KRKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120
            RKE+  LS GDV KIF+QAILG+T+GLN YGLGVKYTSATLGAAAFNCVPVTTFFFALI
Sbjct: 61  ARKEMGSLSKGDVMKIFVQAILGFTVGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120

Query: 121 SR------RCSS----------CI--------LQRPLFQTTLQFSPFSNPS--ISSFFPK 180
           SR      R +S          CI         + P  +   +F  F      +SS    
Sbjct: 121 SRMEKVNIRKASGMAKIGGLILCIAGVAVLAFYKGPYLKPLFKFHLFQTQQSHVSSKKEW 180

Query: 181 NVDSWLFFAPCFFPFMGFV--AWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEW 240
            +  +L F  C    + +V  AWVL++CPSPLVLTFGQTFSS IQSF VAIA+ERNP EW
Sbjct: 181 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFFVAIAIERNPSEW 240

Query: 241 KLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDG 300
           KLAWNI L AILYCG F + + NYLSSWV+KKKGPVFQAV+TPFNLIFTLIGSEFLLNDG
Sbjct: 241 KLAWNISLAAILYCGAFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 300

Query: 301 VSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDHI 322
           + LGSIIGA+LLVLSLYSVLWGKKKE SC DT+N  +SVP ++++LD+I
Sbjct: 301 IGLGSIIGAILLVLSLYSVLWGKKKEASCLDTKNNTNSVPTERKVLDNI 349

[Close]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL
Match: A0A6J1BU97 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005601 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.8e-107
Identity = 223/353 (63.17%), Postives = 256/353 (72.52%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           M+SK+PY AAIV QI F+GMS+ SKAAFASGMNTY+FLFYRQ  G+LFLLP TI+FKRKE
Sbjct: 1   MESKRPYFAAIVSQIIFSGMSLLSKAAFASGMNTYVFLFYRQAFGSLFLLPLTIFFKRKE 60

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           +  LS+G++WK F+QA LG+TL LN YG+GV YTSATLGAAAFNC+PVTTF FALI R  
Sbjct: 61  VTSLSLGEMWKNFMQASLGFTLALNTYGIGVNYTSATLGAAAFNCLPVTTFLFALILRME 120

Query: 125 ----RCSS----------CI------------LQRPLFQTTLQFSPFSNPSISSFFPKNV 184
               R +S          CI              +PLF   L F       +SS      
Sbjct: 121 KVNIRKTSGMAKVGGIVLCIAGVAVLAFYKGPYMKPLFNYHL-FQTHQKSHLSSH----- 180

Query: 185 DSWLFFAPCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMER 244
            +W+    CFF  +  ++W         VLKTCPSPLVLT  QT SS IQSFVVAIA+ER
Sbjct: 181 KTWII--GCFFLLVSCISWGLWIVLQALVLKTCPSPLVLTCVQTLSSAIQSFVVAIAVER 240

Query: 245 NPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEF 304
           NP +WKL WNIRLIA+LYCG F +CIAN LSSWVIKKKGPVFQAV+TP NLIFTLIGSEF
Sbjct: 241 NPSQWKLGWNIRLIAVLYCGTFVICIANSLSSWVIKKKGPVFQAVNTPLNLIFTLIGSEF 300

Query: 305 LLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDH 321
           LLNDGVSLGSIIGAMLLVLSLYSVLWGK+KEMSC +TENK DS P +KE++ H
Sbjct: 301 LLNDGVSLGSIIGAMLLVLSLYSVLWGKRKEMSCGNTENKSDSAPQEKELIVH 345

[Close]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL
Match: A0A6J1IL99 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478060 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 2.6e-106
Identity = 225/348 (64.66%), Postives = 256/348 (73.56%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           MDS KPY+AAI+CQI+FAGMS+ SKAAFASGMN YIFLFYRQ VGTLFLLP TI+FKR+E
Sbjct: 3   MDSNKPYLAAILCQIAFAGMSLLSKAAFASGMNVYIFLFYRQAVGTLFLLPITIFFKRQE 62

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           IAGLSVG V+KIF+QA LG+TL LN YGLGVKYTSATLGAAAFNC+PVTTFFFALI R  
Sbjct: 63  IAGLSVGVVFKIFMQASLGFTLALNVYGLGVKYTSATLGAAAFNCLPVTTFFFALIFRME 122

Query: 125 ----RCSS----------CILQRPL--FQTTLQFSPFSNPSISSFFPKNVDS---WLFFA 184
               R +S          CI    +  F       PF +  +      +V S   W+F  
Sbjct: 123 EVKIRKASGMAKVGGIIVCIAGVAVLAFYRGPYIKPFFHYHLFETHESHVSSPKTWIF-- 182

Query: 185 PCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKL 244
            CF      ++W         +LKT  SPLVLT GQT SS +QSFVVAIA+ERN  EW+L
Sbjct: 183 GCFLLLFACISWGLWFVLQALILKTGSSPLVLTCGQTLSSALQSFVVAIAVERNTSEWQL 242

Query: 245 AWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVS 304
            WNIRL+++LYCG F +CIAN+LSSWVIKKKGPVFQAV+TP NLIFTLIGS+ LLNDGVS
Sbjct: 243 GWNIRLVSVLYCGTFVICIANFLSSWVIKKKGPVFQAVTTPLNLIFTLIGSQLLLNDGVS 302

Query: 305 LGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENK-IDSVPLDKEMLDHI 322
           LGS IGAMLLVLSLYSVLWGK+KEMSC D E K   SVP +KE LDHI
Sbjct: 303 LGSGIGAMLLVLSLYSVLWGKRKEMSCSDEETKNHPSVPPEKETLDHI 348

[Close]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL
Match: A0A6J1GCN7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111452964 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.8e-106
Identity = 223/348 (64.08%), Postives = 254/348 (72.99%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           MDS KPY+AAI+CQI+FAGMS+ SKAAFASGMN YIFLFYRQ VGTLFLLP TI+FKR+E
Sbjct: 3   MDSNKPYLAAILCQIAFAGMSLLSKAAFASGMNVYIFLFYRQAVGTLFLLPITIFFKRQE 62

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           IA LSVG V+KIF+QA LG+TL LN YGLGVKYTSATLGAAAFNC+PVTTFFFALI R  
Sbjct: 63  IASLSVGVVFKIFMQASLGFTLALNVYGLGVKYTSATLGAAAFNCLPVTTFFFALIFRME 122

Query: 125 ----RCSS----------CILQRPL--FQTTLQFSPFSNPSISSFFPKNVDS---WLFFA 184
               R +S          CI    +  F       PF    +      +V S   W+   
Sbjct: 123 EVKIRKASGMAKVGGIIVCIAGVAVLAFYKGPYMKPFFKHHLFEAHESHVSSPKTWIL-- 182

Query: 185 PCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKL 244
            CF      ++W         +LKT  SPLVLT GQT SS +QSFVVAIA+ERN FEW+L
Sbjct: 183 GCFLLLFACISWGLWFVLQALILKTGSSPLVLTCGQTLSSALQSFVVAIAVERNTFEWQL 242

Query: 245 AWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVS 304
            WNIRL+++LYCG F +CIAN+LSSWVIKKKGPVFQAV+TP NLIFTLIGS+ LLNDGVS
Sbjct: 243 GWNIRLVSVLYCGTFVICIANFLSSWVIKKKGPVFQAVTTPLNLIFTLIGSQLLLNDGVS 302

Query: 305 LGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENK-IDSVPLDKEMLDHI 322
           LGS IGAMLLVLSLYSVLWGK+KEMSC D E K   SVP  KE++DHI
Sbjct: 303 LGSAIGAMLLVLSLYSVLWGKRKEMSCSDEETKNHPSVPPQKEIIDHI 348

[Close]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL
Match: A0A0A0KF97 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485120 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 7.3e-101
Identity = 209/354 (59.04%), Postives = 252/354 (71.19%), Query Frame = 0


Query: 1   MIKEMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYF 60
           M ++M+SKKPY AA++CQ  FAGMS+ SKA+++SGMNT+IF FYRQ VGT+FLLP TIYF
Sbjct: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60

Query: 61  KRKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120
            R E+A LS GD+ KIF+QA LG+T G NAYGLGVKYTSATLGAAAFNC+PVTTFFFA I
Sbjct: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120

Query: 121 -------SRRCSS---------CILQRPL--FQTTLQFSPFSNPSISSFFPKNVDS---W 180
                  +++ S          CI    +  F       P +N  +      +V S   W
Sbjct: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180

Query: 181 LFFAPCFFPFMGFVA---------WVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPF 240
           +    C    +  +A         WVL+TCPSPLV+TFGQTFSS+IQSFVVAIA+ERNP 
Sbjct: 181 IL--GCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPS 240

Query: 241 EWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLN 300
           +WKLAWNI L AILYCG+F + I NYLSSWV+KKKGPVFQAV+TPFNLI TLIGSEFL  
Sbjct: 241 QWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFK 300

Query: 301 DGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENK---IDSVPLDKEMLDHI 322
            G+ LGS IGA+LLVLSLYSVLWGKKKE  CHD  N     +SV +++E+LD+I
Sbjct: 301 YGICLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQVEREILDNI 352

[Close]

BLAST of Lsi01G011280 vs. NCBI nr
Match: XP_008441006.1 (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 7.3e-111
Identity = 227/349 (65.04%), Postives = 263/349 (75.36%), Query Frame = 0


Query: 1   MIKEMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYF 60
           M ++  SKKPY  A+VCQI+FAGMS+FSKAAFASGMNTYIFLFYRQ +GT+ LLP TIYF
Sbjct: 1   MEEKKGSKKPYFGALVCQITFAGMSLFSKAAFASGMNTYIFLFYRQALGTIVLLPLTIYF 60

Query: 61  KRKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120
            RKE+  LS GDV KIF+QAILG+T+GLN YGLGVKYTSATLGAAAFNCVPVTTFFFALI
Sbjct: 61  ARKEMGSLSKGDVMKIFVQAILGFTVGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120

Query: 121 SR------RCSS----------CI--------LQRPLFQTTLQFSPFSNPS--ISSFFPK 180
           SR      R +S          CI         + P  +   +F  F      +SS    
Sbjct: 121 SRMEKVNIRKASGMAKIGGLILCIAGVAVLAFYKGPYLKPLFKFHLFQTQQSHVSSKKEW 180

Query: 181 NVDSWLFFAPCFFPFMGFV--AWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEW 240
            +  +L F  C    + +V  AWVL++CPSPLVLTFGQTFSS IQSF VAIA+ERNP EW
Sbjct: 181 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFFVAIAIERNPSEW 240

Query: 241 KLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDG 300
           KLAWNI L AILYCG F + + NYLSSWV+KKKGPVFQAV+TPFNLIFTLIGSEFLLNDG
Sbjct: 241 KLAWNISLAAILYCGAFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 300

Query: 301 VSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDHI 322
           + LGSIIGA+LLVLSLYSVLWGKKKE SC DT+N  +SVP ++++LD+I
Sbjct: 301 IGLGSIIGAILLVLSLYSVLWGKKKEASCLDTKNNTNSVPTERKVLDNI 349

[Close]

BLAST of Lsi01G011280 vs. NCBI nr
Match: XP_004148640.1 (WAT1-related protein At5g64700 [Cucumis sativus])

HSP 1 Score: 406.8 bits (1044), Expect = 1.8e-109
Identity = 224/349 (64.18%), Postives = 258/349 (73.93%), Query Frame = 0


Query: 1   MIKEMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYF 60
           M K+MDSKKPY  A+VCQ++FAGMS+FSKAAF SGMNTYIFLFYRQ  G++ LLP TIYF
Sbjct: 6   MEKKMDSKKPYFGALVCQLTFAGMSLFSKAAFVSGMNTYIFLFYRQAFGSIVLLPLTIYF 65

Query: 61  KRKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120
            RKE+  LS  DV KIFLQ +LG+T+GLN YGLGVKYTSATLGAAAFNCVPVTTFFFAL+
Sbjct: 66  ARKEMRSLSKADVMKIFLQGLLGFTIGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALL 125

Query: 121 SR------RCSS----------CI--------LQRPLFQTTLQFSPFSNPS--ISSFFPK 180
           SR      R +S          CI         + P  +    F  F      +SS    
Sbjct: 126 SRMEKVNIRKASGMAKVGGIILCIAGVAVLAFYKGPYLKPLFNFHLFQTQQSHVSSKKEW 185

Query: 181 NVDSWLFFAPCFFPFMGFV--AWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEW 240
            +  +L F  C    + +V  AWVL++CPSPLVLTFGQTFSS IQSFVVAIA+ERNP EW
Sbjct: 186 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFVVAIAIERNPSEW 245

Query: 241 KLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDG 300
           KL WNI L AILYCG F + + NYLSSWV+KKKGPVFQAV+TPFNLIFTLIGSEFLLNDG
Sbjct: 246 KLGWNISLAAILYCGTFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 305

Query: 301 VSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDHI 322
           +SLGSIIGA+LLVLSLYSVLWGKKKE SC DTEN  + VP + + L++I
Sbjct: 306 ISLGSIIGAILLVLSLYSVLWGKKKEASCFDTENNTNVVPTETKTLNNI 354

[Close]

BLAST of Lsi01G011280 vs. NCBI nr
Match: XP_022132854.1 (WAT1-related protein At5g64700-like isoform X1 [Momordica charantia])

HSP 1 Score: 399.1 bits (1024), Expect = 3.7e-107
Identity = 223/353 (63.17%), Postives = 256/353 (72.52%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           M+SK+PY AAIV QI F+GMS+ SKAAFASGMNTY+FLFYRQ  G+LFLLP TI+FKRKE
Sbjct: 1   MESKRPYFAAIVSQIIFSGMSLLSKAAFASGMNTYVFLFYRQAFGSLFLLPLTIFFKRKE 60

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           +  LS+G++WK F+QA LG+TL LN YG+GV YTSATLGAAAFNC+PVTTF FALI R  
Sbjct: 61  VTSLSLGEMWKNFMQASLGFTLALNTYGIGVNYTSATLGAAAFNCLPVTTFLFALILRME 120

Query: 125 ----RCSS----------CI------------LQRPLFQTTLQFSPFSNPSISSFFPKNV 184
               R +S          CI              +PLF   L F       +SS      
Sbjct: 121 KVNIRKTSGMAKVGGIVLCIAGVAVLAFYKGPYMKPLFNYHL-FQTHQKSHLSSH----- 180

Query: 185 DSWLFFAPCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMER 244
            +W+    CFF  +  ++W         VLKTCPSPLVLT  QT SS IQSFVVAIA+ER
Sbjct: 181 KTWII--GCFFLLVSCISWGLWIVLQALVLKTCPSPLVLTCVQTLSSAIQSFVVAIAVER 240

Query: 245 NPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEF 304
           NP +WKL WNIRLIA+LYCG F +CIAN LSSWVIKKKGPVFQAV+TP NLIFTLIGSEF
Sbjct: 241 NPSQWKLGWNIRLIAVLYCGTFVICIANSLSSWVIKKKGPVFQAVNTPLNLIFTLIGSEF 300

Query: 305 LLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKEMLDH 321
           LLNDGVSLGSIIGAMLLVLSLYSVLWGK+KEMSC +TENK DS P +KE++ H
Sbjct: 301 LLNDGVSLGSIIGAMLLVLSLYSVLWGKRKEMSCGNTENKSDSAPQEKELIVH 345

[Close]

BLAST of Lsi01G011280 vs. NCBI nr
Match: KAG6604270.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 396.0 bits (1016), Expect = 3.2e-106
Identity = 224/348 (64.37%), Postives = 255/348 (73.28%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           MDSKKPY+AAI+CQI+FAGMS+ SKAAFASGMN YIFLFYRQ VGTLFLLP TI+FKR+E
Sbjct: 1   MDSKKPYLAAILCQIAFAGMSLLSKAAFASGMNVYIFLFYRQAVGTLFLLPITIFFKRQE 60

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           IA LSVG V+KIF+QA LG+TL LN YGLGVKYTSATLGAAAFNC+PVTTFFFALI R  
Sbjct: 61  IASLSVGVVFKIFMQASLGFTLALNVYGLGVKYTSATLGAAAFNCLPVTTFFFALIFRME 120

Query: 125 ----RCSS----------CILQRPL--FQTTLQFSPFSNPSISSFFPKNVDS---WLFFA 184
               R +S          CI    +  F       PF    +      +V S   W+   
Sbjct: 121 EVKIRKASGMAKVGGIIVCIAGVAVLAFYKGPYMKPFFKHHLFEAHESHVSSPKTWIL-- 180

Query: 185 PCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKL 244
            CF      ++W         +LKT  SPLVLT GQT SS +QSFVVAIA+ERN FEW+L
Sbjct: 181 GCFLLLFACISWGLWFVLQALILKTGSSPLVLTCGQTLSSALQSFVVAIAVERNTFEWQL 240

Query: 245 AWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVS 304
            WNIRL+++LYCG F +CIAN+LSSWVIKKKGPVFQAV+TP NLIFTLIGS+ LLNDGVS
Sbjct: 241 GWNIRLVSVLYCGTFVICIANFLSSWVIKKKGPVFQAVTTPLNLIFTLIGSQLLLNDGVS 300

Query: 305 LGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENK-IDSVPLDKEMLDHI 322
           LGS IGAMLLVLSLYSVLWGK+KEMSC D E K   SVP  KE++DHI
Sbjct: 301 LGSAIGAMLLVLSLYSVLWGKRKEMSCSDEETKNHPSVPPQKEVIDHI 346

[Close]

BLAST of Lsi01G011280 vs. NCBI nr
Match: XP_022977911.1 (WAT1-related protein At5g64700-like [Cucurbita maxima])

HSP 1 Score: 395.2 bits (1014), Expect = 5.4e-106
Identity = 225/348 (64.66%), Postives = 256/348 (73.56%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           MDS KPY+AAI+CQI+FAGMS+ SKAAFASGMN YIFLFYRQ VGTLFLLP TI+FKR+E
Sbjct: 3   MDSNKPYLAAILCQIAFAGMSLLSKAAFASGMNVYIFLFYRQAVGTLFLLPITIFFKRQE 62

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISR-- 124
           IAGLSVG V+KIF+QA LG+TL LN YGLGVKYTSATLGAAAFNC+PVTTFFFALI R  
Sbjct: 63  IAGLSVGVVFKIFMQASLGFTLALNVYGLGVKYTSATLGAAAFNCLPVTTFFFALIFRME 122

Query: 125 ----RCSS----------CILQRPL--FQTTLQFSPFSNPSISSFFPKNVDS---WLFFA 184
               R +S          CI    +  F       PF +  +      +V S   W+F  
Sbjct: 123 EVKIRKASGMAKVGGIIVCIAGVAVLAFYRGPYIKPFFHYHLFETHESHVSSPKTWIF-- 182

Query: 185 PCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKL 244
            CF      ++W         +LKT  SPLVLT GQT SS +QSFVVAIA+ERN  EW+L
Sbjct: 183 GCFLLLFACISWGLWFVLQALILKTGSSPLVLTCGQTLSSALQSFVVAIAVERNTSEWQL 242

Query: 245 AWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVS 304
            WNIRL+++LYCG F +CIAN+LSSWVIKKKGPVFQAV+TP NLIFTLIGS+ LLNDGVS
Sbjct: 243 GWNIRLVSVLYCGTFVICIANFLSSWVIKKKGPVFQAVTTPLNLIFTLIGSQLLLNDGVS 302

Query: 305 LGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENK-IDSVPLDKEMLDHI 322
           LGS IGAMLLVLSLYSVLWGK+KEMSC D E K   SVP +KE LDHI
Sbjct: 303 LGSGIGAMLLVLSLYSVLWGKRKEMSCSDEETKNHPSVPPEKETLDHI 348

[Close]

BLAST of Lsi01G011280 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-55
Identity = 133/354 (37.57%), Postives = 187/354 (52.82%), Query Frame = 0


Query: 4   EMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRK 63
           +M+SKKPY+   + Q+ +  M + SKA F  GMNT++F+FYRQ   T+FL P   +F+RK
Sbjct: 2   DMESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERK 61

Query: 64  EIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRR 123
               LS     KIF+ ++ G TL L+  G+ + YTSATL AA    +P  TFF AL+   
Sbjct: 62  SAPPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGM 121

Query: 124 -------------------CSSCILQRPLFQTTLQFSPF-------------SNPSISSF 183
                              C   ++   +++  L   P              +NP   S 
Sbjct: 122 ERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVS- 181

Query: 184 FPKNVDSWLFFAPCFFPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVA 243
                 SWL    C       + W         VLK  PS L  T      S+IQSFV+A
Sbjct: 182 --GGSTSWL--KGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIA 241

Query: 244 IAMERNPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTL 303
           IA+ER+   WKL WN+RL+A++YCG     +A YL SWVI+K+GPVF ++ TP +L+FTL
Sbjct: 242 IALERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTL 301

Query: 304 IGSEFLLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDSVPLDKE 317
           + S  LL + +SLGSI+G +LL++ LY VLWGK +E    +  +  D + L KE
Sbjct: 302 LSSAILLCEIISLGSIVGGLLLIIGLYCVLWGKSRE----EKNSGDDKIDLQKE 346

[Close]

BLAST of Lsi01G011280 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 155.6 bits (392), Expect = 6.7e-38
Identity = 113/320 (35.31%), Postives = 163/320 (50.94%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           M   K  +A +  QI +AGM + SK A + G N ++F+FYRQ    L L P   + +  +
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRRC 124
            + LS   + KIF  ++ G TL LN Y + ++ T+AT  AA  N +P  TF  AL+ R  
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 125 SSCILQ-RPLFQTT---------LQFSPFSNPSI-----SSFFP-----------KNVDS 184
           +  + +   + + T         L F+    PS+     SS  P           K   +
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSLINHYNSSTIPNGTVPSTKNSVKGSIT 181

Query: 185 WLFFAPCFFPFMGFVAWVLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPFEWKLAWNI 244
            L    C+  ++   + V+K  P+ L L   Q   S IQS V A+A+ RNP  WK+ + +
Sbjct: 182 MLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFGL 241

Query: 245 RLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLGSI 299
            L+++ YCGI    +  +L  W I+KKGPVF A+ TP  LI T I S FL  +   LGS+
Sbjct: 242 PLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGSV 301

[Close]

BLAST of Lsi01G011280 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 150.2 bits (378), Expect = 2.8e-36
Identity = 108/344 (31.40%), Postives = 166/344 (48.26%), Query Frame = 0


Query: 5   MDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIYFKRKE 64
           + S KPY A I  Q  +AGM++ +K +  +GM+ Y+ + YR  + T  + P   +F+RK 
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 65  IAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFFFALISRR- 124
              ++     ++F+  +LG  +  N Y +G+KYTS T   A  N +P  TF  A++ R  
Sbjct: 73  QPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRME 132

Query: 125 --------CSS---------------CILQRP---LFQTT---LQFSPFSNPSIS----- 184
                   C +                I + P   LF T    +Q S  +N + S     
Sbjct: 133 MLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSS 192

Query: 185 -SFFPKNVDSWLFFAPCFFPFMGFVAWVLKT-CPSPLVLTFGQTFSSTIQSFVVAIAMER 244
              F K     +F    +       A +LKT     L LT    F  T+Q+  V   ME 
Sbjct: 193 DKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEH 252

Query: 245 NPFEWKLAWNIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEF 304
           NP  W++ W++ L+A  Y GI A  I+ Y+   V+KK+GPVF    +P  ++   +   F
Sbjct: 253 NPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSF 312

Query: 305 LLNDGVSLGSIIGAMLLVLSLYSVLWGKKKEMSCHDTE-NKIDS 311
           +L + + LG +IGA+L+V+ LY+VLWGK+KE      E  KIDS
Sbjct: 313 VLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDS 356

[Close]

BLAST of Lsi01G011280 vs. TAIR 10
Match: AT4G28040.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 145.6 bits (366), Expect = 6.9e-35
Identity = 96/334 (28.74%), Postives = 164/334 (49.10%), Query Frame = 0


Query: 4   EMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIY--FK 63
           E+   K  +A ++ Q + AG+++F+KAAF  G+N  +F+ YRQ + TLF+ P +    ++
Sbjct: 2   EISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWR 61

Query: 64  RKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFF----- 123
           ++    L V   W + L A++G T+  NAY  G+  +S+++  A  N +P  TF      
Sbjct: 62  KENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIV 121

Query: 124 -FALISRRCSSCILQ---------RPLFQTTLQFSPFSNPSISSFFPKNVDSWLFFAPCF 183
            F  I RR    + +           +  T L+     N  +      N D+  +   CF
Sbjct: 122 GFESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNALL------NQDNTAWLLGCF 181

Query: 184 FPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPF-EWKLAW 243
           F  +   AW         +   CP  L  +    F +TI SF+VA+A+       WKL  
Sbjct: 182 FLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPPWKLDS 241

Query: 244 NIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLG 303
            ++L   +Y G F + I+ +L +W++ +KGPVF A+  P + +         L +   LG
Sbjct: 242 FLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKEQTYLG 301

Query: 304 SIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDS 311
           S++GA+ ++L LY VLWGK ++     T+ K+++
Sbjct: 302 SLLGALAIILGLYIVLWGKSEDYQEESTDLKLEN 328

[Close]

BLAST of Lsi01G011280 vs. TAIR 10
Match: AT4G28040.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 145.6 bits (366), Expect = 6.9e-35
Identity = 96/334 (28.74%), Postives = 164/334 (49.10%), Query Frame = 0


Query: 4   EMDSKKPYIAAIVCQISFAGMSVFSKAAFASGMNTYIFLFYRQTVGTLFLLPPTIY--FK 63
           E+   K  +A ++ Q + AG+++F+KAAF  G+N  +F+ YRQ + TLF+ P +    ++
Sbjct: 2   EISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWR 61

Query: 64  RKEIAGLSVGDVWKIFLQAILGYTLGLNAYGLGVKYTSATLGAAAFNCVPVTTFF----- 123
           ++    L V   W + L A++G T+  NAY  G+  +S+++  A  N +P  TF      
Sbjct: 62  KENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIV 121

Query: 124 -FALISRRCSSCILQ---------RPLFQTTLQFSPFSNPSISSFFPKNVDSWLFFAPCF 183
            F  I RR    + +           +  T L+     N  +      N D+  +   CF
Sbjct: 122 GFESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNALL------NQDNTAWLLGCF 181

Query: 184 FPFMGFVAW---------VLKTCPSPLVLTFGQTFSSTIQSFVVAIAMERNPF-EWKLAW 243
           F  +   AW         +   CP  L  +    F +TI SF+VA+A+       WKL  
Sbjct: 182 FLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPPWKLDS 241

Query: 244 NIRLIAILYCGIFAMCIANYLSSWVIKKKGPVFQAVSTPFNLIFTLIGSEFLLNDGVSLG 303
            ++L   +Y G F + I+ +L +W++ +KGPVF A+  P + +         L +   LG
Sbjct: 242 FLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKEQTYLG 301

Query: 304 SIIGAMLLVLSLYSVLWGKKKEMSCHDTENKIDS 311
           S++GA+ ++L LY VLWGK ++     T+ K+++
Sbjct: 302 SLLGALAIILGLYIVLWGKSEDYQEESTDLKLEN 328

The following BLAST results are available for this feature:

BLAST of Lsi01G011280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGG3	1.7e-54	37.57	WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2...	[more]
Q6NMB7	9.4e-37	35.31	WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2...	[more]
Q9FL41	4.0e-35	31.40	WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2...	[more]
Q9SUD5	9.7e-34	28.74	WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2...	[more]
Q9ZUS1	2.2e-33	30.79	WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2...	[more]

BLAST of Lsi01G011280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B374	3.5e-111	65.04	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485250 PE=3 SV=1	[more]
A0A6J1BU97	1.8e-107	63.17	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005601 PE=3 SV=1	[more]
A0A6J1IL99	2.6e-106	64.66	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478060 PE=3 SV=1	[more]
A0A6J1GCN7	5.8e-106	64.08	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111452964 PE=3 SV=1	[more]
A0A0A0KF97	7.3e-101	59.04	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485120 PE=3 SV=1	[more]

BLAST of Lsi01G011280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441006.1	7.3e-111	65.04	PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo]	[more]
XP_004148640.1	1.8e-109	64.18	WAT1-related protein At5g64700 [Cucumis sativus]	[more]
XP_022132854.1	3.7e-107	63.17	WAT1-related protein At5g64700-like isoform X1 [Momordica charantia]	[more]
KAG6604270.1	3.2e-106	64.37	WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022977911.1	5.4e-106	64.66	WAT1-related protein At5g64700-like [Cucurbita maxima]	[more]

BLAST of Lsi01G011280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64700.1	1.2e-55	37.57	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.1	6.7e-38	35.31	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G07050.1	2.8e-36	31.40	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.1	6.9e-35	28.74	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.2	6.9e-35	28.74	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 9..119 e-value: 9.5E-13 score: 48.5 coord: 158..292 e-value: 1.7E-8 score: 34.7
None	No IPR available	PANTHER	PTHR31218:SF166	WAT1-RELATED PROTEIN	coord: 172..307
None	No IPR available	PANTHER	PTHR31218:SF166	WAT1-RELATED PROTEIN	coord: 9..123
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 44..119
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 214..297
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 9..123
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 172..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G011280.1	Lsi01G011280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity