Lsi01G010530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G010530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCalcium uniporter protein
Locationchr01: 8723465 .. 8725276 (-)
RNA-Seq ExpressionLsi01G010530
SyntenyLsi01G010530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAATTCCAACTTTATTTCCCTCTGTAGCTTTTTTTTCGTCTTTCTTTTTGTTTTTCTTCTGTTAATGGCGTCCAGAAAAACCCTAGCTCGCCGCCTCTTCAACGCCTCCAATCGGCTACTTTTCCGAAATTCCGCCGCCGATAAATCCATTAACCTCCCACTCGATCCCGGAGATCATGGAATTTCTCGCCGATTTCTTCATCGGAGGCCCAATTTTCCCCCTCCCGCCACATTCACCCGATCGCTTCCGCCATTGCCGGTCGGATTCGGGAATGTGATGGAACAGCTCGTTTCGCGCGATAGGATTCTTCTAGATGGACTGAAGCCTCCGATGCCGGCGCCGGGGCAGGTTCCGGATGAATTGGAGGGACTTACGGTGGAGGAGACGAGGAAGTTAGTGAAATTGACGGAGGTGGTGAGATTGAAGAGGAAATTGAAGCAGATTCCGAGGAGTTGGATTACGTATCAGGAATTCGTTCGGATTTGTGGAGAAGATTGTTCAGATAATAATGGAGATAATGAAAATTATGGGGTTGAGTTGGCCAAGAGGCTTGATCATTCCGGTGCCGTCATCGTTTTGGGCAACTTCGTCTTTCTCGATCCCGAGCAGGTATTTCCAGTTAAATCACCAATCAATTCAAAAACTTAAAATAAAGAAAATAAAGAGTTAAGAACCTCCAAATATGGTGGTAATTGCAATTAGGCTCTTAAACTTCTAAACATATAAATTGAACCCTCAACCATCATATCTAGGGTTTTTGCAATTTGCCCTAATATTAAATTACAAAGAAAGCCCACAAGTGAATTGTTGATATGATTTTAAATTTAAACATAATTCCTTTTGACTTTTATAGATGGCAAAATCAATAGCTAGCTTGATTCCCACGCTAGGAATCAACAACGAAGTCTCAACAGGCAACCAGGAGCTCGAAGAAATGGAAAAACAGAAGGCCATGATCGACGGCAAGGCCGATCAACAAGCTCGCCGTGAATTCCGGTGGGGCCTCGGGTTTCTAGTGGCTCAAACCGCCGCCCTAATGAGGTTGACATTTTGGGAGTTAACTTGGGACGTAATGGAGCCAATTTGCTACTTCATAACGTCAACATATTTCATGGGTGGTTATGCCTTCTTTCTAACCACTTCCAAAGAGCCTTCTTTTGAAGGGATTTACCAAAGCCGCTTCATCGCTAAGCAAAAGCATCTCATGAAGCTCCACAATTTTGACATCCATAAATACAATCGCCTTCGAGGACATGATGATTCCAATCAATTTCCTTCTTCAAATCATCAGTTTTTTTAAAGCTAGAGAGTACATATACAAAAATTATTGAGAGCATTTTAAAGTTTTGAAAATCATTGTTTCCTACTATTTTATATTCTTTTTGCGACATGACTTTTGAGCGGTTGTAGTACAAAGAAACGAGTCTAAGTTTCATACATTCTTTAAAGTAAACATATACTATCACTATGAATGTTGACACTTGTTGTGAAAGAATTGTCGCCGAATAGATTGAGAATAATAATAATGAAGAAAAAAAAAAGAAAATAACACGTGAAAAACGCCTACACAAATTAGGGTAAGAATTACGGGCTAAAATAGAAAAAATCCACTATATAAATATAGTTACCATCTTTATTTCTTATAGAGAAAAAACAAACTAAGAAACTCTATTTTATCTTACAATTCACTTGTTTTGGGAATCTGGATGGATTGCCAACAATGGGTACTCACCTATTCATATTCCACGTAAACTTGACCATGAAACAAATCCAATAGCTCCAAATTGACCACGAAACAAATCCAATAGC

mRNA sequence

TTCAATTCCAACTTTATTTCCCTCTGTAGCTTTTTTTTCGTCTTTCTTTTTGTTTTTCTTCTGTTAATGGCGTCCAGAAAAACCCTAGCTCGCCGCCTCTTCAACGCCTCCAATCGGCTACTTTTCCGAAATTCCGCCGCCGATAAATCCATTAACCTCCCACTCGATCCCGGAGATCATGGAATTTCTCGCCGATTTCTTCATCGGAGGCCCAATTTTCCCCCTCCCGCCACATTCACCCGATCGCTTCCGCCATTGCCGGTCGGATTCGGGAATGTGATGGAACAGCTCGTTTCGCGCGATAGGATTCTTCTAGATGGACTGAAGCCTCCGATGCCGGCGCCGGGGCAGGTTCCGGATGAATTGGAGGGACTTACGGTGGAGGAGACGAGGAAGTTAGTGAAATTGACGGAGGTGGTGAGATTGAAGAGGAAATTGAAGCAGATTCCGAGGAGTTGGATTACGTATCAGGAATTCGTTCGGATTTGTGGAGAAGATTGTTCAGATAATAATGGAGATAATGAAAATTATGGGGTTGAGTTGGCCAAGAGGCTTGATCATTCCGGTGCCGTCATCGTTTTGGGCAACTTCGTCTTTCTCGATCCCGAGCAGATGGCAAAATCAATAGCTAGCTTGATTCCCACGCTAGGAATCAACAACGAAGTCTCAACAGGCAACCAGGAGCTCGAAGAAATGGAAAAACAGAAGGCCATGATCGACGGCAAGGCCGATCAACAAGCTCGCCGTGAATTCCGGTGGGGCCTCGGGTTTCTAGTGGCTCAAACCGCCGCCCTAATGAGGTTGACATTTTGGGAGTTAACTTGGGACGTAATGGAGCCAATTTGCTACTTCATAACGTCAACATATTTCATGGGTGGTTATGCCTTCTTTCTAACCACTTCCAAAGAGCCTTCTTTTGAAGGGATTTACCAAAGCCGCTTCATCGCTAAGCAAAAGCATCTCATGAAGCTCCACAATTTTGACATCCATAAATACAATCGCCTTCGAGGACATGATGATTCCAATCAATTTCCTTCTTCAAATCATCAGTTTTTTTAAAGCTAGAGAGTACATATACAAAAATTATTGAGAGCATTTTAAAGTTTTGAAAATCATTGTTTCCTACTATTTTATATTCTTTTTGCGACATGACTTTTGAGCGGTTGTAGTACAAAGAAACGAGTCTAAGTTTCATACATTCTTTAAAGTAAACATATACTATCACTATGAATGTTGACACTTGTTGTGAAAGAATTGTCGCCGAATAGATTGAGAATAATAATAATGAAGAAAAAAAAAAGAAAATAACACGTGAAAAACGCCTACACAAATTAGGGTAAGAATTACGGGCTAAAATAGAAAAAATCCACTATATAAATATAGTTACCATCTTTATTTCTTATAGAGAAAAAACAAACTAAGAAACTCTATTTTATCTTACAATTCACTTGTTTTGGGAATCTGGATGGATTGCCAACAATGGGTACTCACCTATTCATATTCCACGTAAACTTGACCATGAAACAAATCCAATAGCTCCAAATTGACCACGAAACAAATCCAATAGC

Coding sequence (CDS)

ATGGCGTCCAGAAAAACCCTAGCTCGCCGCCTCTTCAACGCCTCCAATCGGCTACTTTTCCGAAATTCCGCCGCCGATAAATCCATTAACCTCCCACTCGATCCCGGAGATCATGGAATTTCTCGCCGATTTCTTCATCGGAGGCCCAATTTTCCCCCTCCCGCCACATTCACCCGATCGCTTCCGCCATTGCCGGTCGGATTCGGGAATGTGATGGAACAGCTCGTTTCGCGCGATAGGATTCTTCTAGATGGACTGAAGCCTCCGATGCCGGCGCCGGGGCAGGTTCCGGATGAATTGGAGGGACTTACGGTGGAGGAGACGAGGAAGTTAGTGAAATTGACGGAGGTGGTGAGATTGAAGAGGAAATTGAAGCAGATTCCGAGGAGTTGGATTACGTATCAGGAATTCGTTCGGATTTGTGGAGAAGATTGTTCAGATAATAATGGAGATAATGAAAATTATGGGGTTGAGTTGGCCAAGAGGCTTGATCATTCCGGTGCCGTCATCGTTTTGGGCAACTTCGTCTTTCTCGATCCCGAGCAGATGGCAAAATCAATAGCTAGCTTGATTCCCACGCTAGGAATCAACAACGAAGTCTCAACAGGCAACCAGGAGCTCGAAGAAATGGAAAAACAGAAGGCCATGATCGACGGCAAGGCCGATCAACAAGCTCGCCGTGAATTCCGGTGGGGCCTCGGGTTTCTAGTGGCTCAAACCGCCGCCCTAATGAGGTTGACATTTTGGGAGTTAACTTGGGACGTAATGGAGCCAATTTGCTACTTCATAACGTCAACATATTTCATGGGTGGTTATGCCTTCTTTCTAACCACTTCCAAAGAGCCTTCTTTTGAAGGGATTTACCAAAGCCGCTTCATCGCTAAGCAAAAGCATCTCATGAAGCTCCACAATTTTGACATCCATAAATACAATCGCCTTCGAGGACATGATGATTCCAATCAATTTCCTTCTTCAAATCATCAGTTTTTTTAA

Protein sequence

MASRKTLARRLFNASNRLLFRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPATFTRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQFF
Homology
BLAST of Lsi01G010530 vs. ExPASy Swiss-Prot
Match: Q9LVR5 (Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g66650 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.8e-66
Identity = 146/323 (45.20%), Postives = 199/323 (61.61%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLL----FRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPAT 60
           M+S+K+L + LFN S               KS  +P D GD GI RRFLH+R  F P   
Sbjct: 1   MSSKKSLVQSLFNISKTYSRISGLTRMRPTKSGGIPPDAGDSGIRRRFLHKRAFFSPEI- 60

Query: 61  FTRSLPPLPVGFGNVMEQ-----LVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKL 120
                  +P G GN+ME+     L + +RI LD + PP       P+    +TVE+ +KL
Sbjct: 61  -------VPKG-GNLMEKLRELTLSNNNRIRLDEMLPPPSPKKSSPEFFPAVTVEDVKKL 120

Query: 121 VKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIV 180
           ++  E+  +K KL++I ++W+ Y EFVR+CGE  SD        G  +A  LD +G VIV
Sbjct: 121 MRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSSD-----PEQGNRVANMLDEAGNVIV 180

Query: 181 LGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRW 240
           LG  V L PE++  ++A LIPTL  + +  T  QE E++E  K+ ID +AD   R+E   
Sbjct: 181 LGKLVCLKPEELTSAMAGLIPTLEPSLDAET-RQEFEQLEIIKSDIDKRADDLVRKELWA 240

Query: 241 GLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSR 300
           GLG ++AQT    RLTFWEL+WDVMEPIC+++TSTYFM GYAFFL TSKEPSFEG Y+SR
Sbjct: 241 GLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGYAFFLRTSKEPSFEGFYKSR 300

Query: 301 FIAKQKHLMKLHNFDIHKYNRLR 315
           F  KQK L+K+ +FDI ++ +L+
Sbjct: 301 FETKQKRLIKMLDFDIDRFTKLQ 308

BLAST of Lsi01G010530 vs. ExPASy Swiss-Prot
Match: O64823 (Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g23790 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-64
Identity = 148/336 (44.05%), Postives = 202/336 (60.12%), Query Frame = 0

Query: 1   MASRKTLARRLFNASN------------------RLLFRNSAADKSINLPLDPGDHGISR 60
           MA RK L+++LFN +N                  R    N + D +   P +PGD  +SR
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAP-EPGDLAMSR 60

Query: 61  RFLHRRPNFPPPATFTRSLPPLPVGFGNVMEQL----VSRDRILLDGLKPPMPAPGQVPD 120
           RF+H      P       +  +PVG  +++E+L     S+DRI LDGL P        P+
Sbjct: 61  RFMHNSAMIRP-----AEIMQMPVG-ESLIEKLREIDGSKDRIRLDGLSP--------PE 120

Query: 121 ELEGLTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVE 180
               LTV +T+KL++  ++  +K KL++  RSW+ Y+EFV +CGE  SD      + G +
Sbjct: 121 RETSLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSD-----PDLGSK 180

Query: 181 LAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMID 240
           +AK LD S  VIVLG+ V + P+Q+ KSI  L+P   I+N       E +E+E +KA+ID
Sbjct: 181 IAKMLDDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVID 240

Query: 241 GKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTT 300
            KA    R+E   GLG+L+ QTA  MRLTFWEL+WDVMEPIC+++TS YFM GYAFFL T
Sbjct: 241 VKAHTLVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRT 300

Query: 301 SKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRLR 315
           SKEPSFEG YQSRF AKQ+ LM  + FD+ +YN L+
Sbjct: 301 SKEPSFEGFYQSRFEAKQRKLMNEYEFDLERYNELK 316

BLAST of Lsi01G010530 vs. ExPASy Swiss-Prot
Match: Q1PE15 (Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g36820 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.9e-58
Identity = 140/332 (42.17%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 4   RKTLARRLFN----ASNRLLFRNSAADKSI----NLPLD---------PGDHGISRRFLH 63
           +K L+ RLFN    AS  L+   +++  S+     +P D         PGD  IS+RFL+
Sbjct: 5   KKLLSNRLFNMSKTASQSLMNCRTSSSSSLAMRTRVPKDIGEATIDPEPGDLTISQRFLN 64

Query: 64  RRPNFPPPATFTRSLPPLPVGFGNVMEQL----VSRDRILLDGLKPPMPAPGQVPDELEG 123
           +   F      T S   + +G  ++ME+L    +++DRI LDGL  P        +E  G
Sbjct: 65  K---FSMNGIDTTS--KMSIG-ESLMEKLKEMDMNKDRIRLDGLSHP-------KEETLG 124

Query: 124 LTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKR 183
           LTV++ +KL++  E+  +K KL +  + WI Y +F+ +C +   D      + G  +AK 
Sbjct: 125 LTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLD-----PSQGALIAKM 184

Query: 184 LDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKAD 243
           LD SG VIV+GN V L P Q+ KSI  L+P   I+N      +EL E+E  K +ID KA 
Sbjct: 185 LDDSGNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAH 244

Query: 244 QQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEP 303
              RRE   GLG+L+ QTA  MRLTFW+LTWDVMEPIC++++S YFM GY FFL TS+EP
Sbjct: 245 SLVRRELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREP 304

Query: 304 SFEGIYQSRFIAKQKHLMKLHNFDIHKYNRLR 315
           SF+G YQSRF AKQ+ LM+  +FD+ +Y+ L+
Sbjct: 305 SFQGFYQSRFEAKQRKLMQSEDFDVGRYDELK 318

BLAST of Lsi01G010530 vs. ExPASy Swiss-Prot
Match: Q9FJV7 (Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g42610 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-49
Identity = 106/235 (45.11%), Postives = 154/235 (65.53%), Query Frame = 0

Query: 79  DRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFV 138
           DRI+   ++   P P +    + GL+V ET+KL++L ++ ++K +L++IP+S ++Y EF+
Sbjct: 51  DRIVGIRIESVSP-PRREETPVMGLSVSETKKLLRLYQMEKVKERLREIPKSSVSYWEFI 110

Query: 139 RICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINN 198
           +IC E C      N+  G ++AK LDHSG V+VLG+ VFL P Q+AKS+ ++I    +  
Sbjct: 111 QICCESCG-----NDEQGSQMAKSLDHSGCVVVLGDIVFLHPHQIAKSMEAMIKQTSVLP 170

Query: 199 EVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEP 258
                 +EL ++   K  ID +A +  + E   GLGFL  QT   MRLTFWEL+WDVMEP
Sbjct: 171 N-DPRKEELVQLATTKKSIDIEARRIVQAELYCGLGFLAVQTIGFMRLTFWELSWDVMEP 230

Query: 259 ICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRL 314
           IC+F+T+ +F+ GY FFL TS EPSFEG+++ RF  KQK LM+ H FD  +YN L
Sbjct: 231 ICFFVTTIHFILGYIFFLRTSTEPSFEGLFRQRFKTKQKKLMERHGFDFLRYNEL 278

BLAST of Lsi01G010530 vs. ExPASy Swiss-Prot
Match: Q8VYR0 (Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g57610 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 6.3e-28
Identity = 73/217 (33.64%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 98  DELEGLTVEETRKLVKLTEVVRLKRKLKQI-PRSWITYQEFVRICGEDCSDNNGDNENYG 157
           DE + +TV E +KL++L  V  +K+KL  +  +  +TY   +    +        +E + 
Sbjct: 41  DEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIE-ASQGLGIARSLDEAHA 100

Query: 158 VELAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAM 217
              A+ LD +G +++  + V+L P ++   I   +P LG+N +     +E ++M   K  
Sbjct: 101 --FARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVP-LGLNPDDELIREEFDKMRSMKEE 160

Query: 218 IDGKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFL 277
           ID  A QQ R+    GLG+ V Q    +RLTFWE +WDVMEPI +F T+T  + GYA+FL
Sbjct: 161 IDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGIIVGYAYFL 220

Query: 278 TTSKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRL 314
            TS++P+++   +  F+++Q+ L+K H FD  ++  L
Sbjct: 221 MTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKEL 253

BLAST of Lsi01G010530 vs. ExPASy TrEMBL
Match: A0A5D3CNM0 (Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005480 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.0e-150
Identity = 281/332 (84.64%), Postives = 294/332 (88.55%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS+K L RRLFNA+NRLLFRNS   +   S NLPLDPGDHGI RRFLHR+PNFPPP+TF
Sbjct: 1   MASKKALPRRLFNAANRLLFRNSPNPRPHISNNLPLDPGDHGIFRRFLHRKPNFPPPSTF 60

Query: 61  TRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEV 120
           TRS   LPVG GNVMEQL SRDRILLDGLKPP  AP  VP ELEGLTVEETRKLVKLTEV
Sbjct: 61  TRS---LPVGLGNVMEQLFSRDRILLDGLKPPTTAPASVPSELEGLTVEETRKLVKLTEV 120

Query: 121 VRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVF 180
           VRLKRKLK+IPRSWITYQEFVRICGEDC D   DNE YGVELAKRLDHSGAVIVLGN VF
Sbjct: 121 VRLKRKLKEIPRSWITYQEFVRICGEDCWD---DNEEYGVELAKRLDHSGAVIVLGNLVF 180

Query: 181 LDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLV 240
           LDPEQMAKSIASLIPTL +N+E S GN+ELEEMEKQKAMID +ADQQ RRE RWGLGFLV
Sbjct: 181 LDPEQMAKSIASLIPTLLMNDEGSEGNEELEEMEKQKAMIDVEADQQVRRELRWGLGFLV 240

Query: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQK 300
           AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQ+RF+AKQK
Sbjct: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQTRFMAKQK 300

Query: 301 HLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           HLMKLHNFDIHKYNRLRGH  SN   S+NH F
Sbjct: 301 HLMKLHNFDIHKYNRLRGH-HSNPLTSTNHHF 325

BLAST of Lsi01G010530 vs. ExPASy TrEMBL
Match: A0A1S3B0G9 (Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103484705 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.7e-150
Identity = 281/332 (84.64%), Postives = 293/332 (88.25%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS+K L RRLFNA+NRLLFRNS   +   S NLPLDPGDHGI RRFLHR+PNFPPP+TF
Sbjct: 1   MASKKALPRRLFNAANRLLFRNSPNPRPHISNNLPLDPGDHGIFRRFLHRKPNFPPPSTF 60

Query: 61  TRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEV 120
           TRS   LPVG GNVMEQL SRDRILLDGLKPP  AP  VP ELEGLTVEETRKLVKLTEV
Sbjct: 61  TRS---LPVGLGNVMEQLFSRDRILLDGLKPPTTAPASVPSELEGLTVEETRKLVKLTEV 120

Query: 121 VRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVF 180
           VRLKRKLK+IPRSWITYQEFVRICGEDC D   DNE YGVELAKRLDHSGAVIVLGN VF
Sbjct: 121 VRLKRKLKEIPRSWITYQEFVRICGEDCWD---DNEEYGVELAKRLDHSGAVIVLGNLVF 180

Query: 181 LDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLV 240
           LDPEQMAKSIASLIPTL +NNE S GN+ELEEMEKQKAMID +ADQQ RRE RWGLGFLV
Sbjct: 181 LDPEQMAKSIASLIPTLLMNNEGSEGNEELEEMEKQKAMIDVEADQQVRRELRWGLGFLV 240

Query: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQK 300
           AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQ+RF+AKQK
Sbjct: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQTRFMAKQK 300

Query: 301 HLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           HLMKLHNFDIHKYNRLRGH  SN   S+ H F
Sbjct: 301 HLMKLHNFDIHKYNRLRGH-HSNPLTSTYHHF 325

BLAST of Lsi01G010530 vs. ExPASy TrEMBL
Match: A0A0A0KI13 (Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G487650 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.2e-148
Identity = 279/334 (83.53%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS K L RRLFNA+NRLLF NS   +   S NLPLDPGDH I RRFLHRRP+FPPP+TF
Sbjct: 1   MASNKALPRRLFNAANRLLFPNSPNPRPHVSNNLPLDPGDHAIFRRFLHRRPSFPPPSTF 60

Query: 61  TR--SLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLT 120
           TR  SLPPLPVGFGNVMEQL+SRDRILLDGLKPP      VP ELEGLTVEETRKLVKLT
Sbjct: 61  TRSSSLPPLPVGFGNVMEQLLSRDRILLDGLKPPT----SVPSELEGLTVEETRKLVKLT 120

Query: 121 EVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNF 180
           EVVRLKRKLK+IPRSWITY+EFVRICGEDCSD    NE+YGVELAKRLDHSGAVIVLGNF
Sbjct: 121 EVVRLKRKLKEIPRSWITYREFVRICGEDCSDG---NEDYGVELAKRLDHSGAVIVLGNF 180

Query: 181 VFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGF 240
           VFLDPEQMAKSIASLIP+L   NE S GN+ELEEMEKQK MID +ADQQ RRE RWGLGF
Sbjct: 181 VFLDPEQMAKSIASLIPSLVNQNEGSKGNEELEEMEKQKTMIDMEADQQVRRELRWGLGF 240

Query: 241 LVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAK 300
           LVAQTAALMRLTFWELTWDVMEPICYFITS+YFMGGYAFFLTTSKEPSFEGIYQSRF+AK
Sbjct: 241 LVAQTAALMRLTFWELTWDVMEPICYFITSSYFMGGYAFFLTTSKEPSFEGIYQSRFMAK 300

Query: 301 QKHLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           QKHLMKLHNFDIHKYNRLRG   SN F S+NH F
Sbjct: 301 QKHLMKLHNFDIHKYNRLRGLHCSNPFTSTNHHF 327

BLAST of Lsi01G010530 vs. ExPASy TrEMBL
Match: A0A6J1GCQ4 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111452996 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 254/320 (79.38%), Postives = 280/320 (87.50%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPATFTRS 60
           MAS KTLARRLFNASNRL F N++A+K   LPLDPGD GI RRFLH+RPNFPPP+TFTRS
Sbjct: 1   MASTKTLARRLFNASNRLFF-NTSANK---LPLDPGDQGIFRRFLHQRPNFPPPSTFTRS 60

Query: 61  LPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRL 120
           LP LP+GFGN MEQLVSRDRILLDGLKPP PAPG    EL+GLTVEETRKLV+L EVVR+
Sbjct: 61  LPSLPIGFGNAMEQLVSRDRILLDGLKPPAPAPG----ELDGLTVEETRKLVRLAEVVRM 120

Query: 121 KRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDP 180
           KRKLK+IP   ITY+EFVRICGED SD    +E+YGVELAKRLD SGAVI+LGN VFL P
Sbjct: 121 KRKLKEIPTGRITYREFVRICGEDNSDG---DEDYGVELAKRLDESGAVIILGNVVFLHP 180

Query: 181 EQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQT 240
           EQ+AKSI  LIPTLG+N+E     +ELEE+EK+KAMIDGKA +Q RRE RWGLGFLVAQT
Sbjct: 181 EQIAKSIGCLIPTLGMNSEDPISKKELEELEKEKAMIDGKAGRQVRRELRWGLGFLVAQT 240

Query: 241 AALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLM 300
           AALMRLTFWEL+WDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEG+YQSRF+AKQK+LM
Sbjct: 241 AALMRLTFWELSWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGLYQSRFVAKQKNLM 300

Query: 301 KLHNFDIHKYNRLRGHDDSN 321
           KLHNFD+HKYNRLR   DSN
Sbjct: 301 KLHNFDVHKYNRLR--SDSN 307

BLAST of Lsi01G010530 vs. ExPASy TrEMBL
Match: A0A6J1IPT8 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111478366 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.3e-129
Identity = 246/320 (76.88%), Postives = 269/320 (84.06%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPATFTRS 60
           MAS K LARRLFNASNRL F  S    S NLPLDP DHGI RRFLH+RPNF PP+TFT S
Sbjct: 1   MASTKALARRLFNASNRLFFNTS----SNNLPLDPRDHGIFRRFLHQRPNFSPPSTFTPS 60

Query: 61  LPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRL 120
           LP LP+GFGN MEQLVSRDRI LDGLKPP       P EL+GLTVEETRKLV+L EVVR+
Sbjct: 61  LPSLPIGFGNAMEQLVSRDRIHLDGLKPP------APGELDGLTVEETRKLVRLAEVVRM 120

Query: 121 KRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDP 180
           KRKLK +P S ITY+EFVRICGED SD    +E+YGVELAKRLD SGAVIV+GN VFL P
Sbjct: 121 KRKLKDVPTSQITYREFVRICGEDYSDG---DEDYGVELAKRLDESGAVIVIGNVVFLHP 180

Query: 181 EQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQT 240
           EQ+AKSI  LIPTLG+N+E     +ELEE+EK+KAMIDGKA +Q RRE RWGLGFLVAQT
Sbjct: 181 EQIAKSIGCLIPTLGMNSEDPISKKELEELEKEKAMIDGKAGRQVRRELRWGLGFLVAQT 240

Query: 241 AALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLM 300
            ALMRLTFWEL+WDVMEPICYFITSTYFMGGYAFFLTTS+EPSFEGIYQSRF+AKQK+LM
Sbjct: 241 VALMRLTFWELSWDVMEPICYFITSTYFMGGYAFFLTTSREPSFEGIYQSRFVAKQKNLM 300

Query: 301 KLHNFDIHKYNRLRGHDDSN 321
           KLHNFD+HKYNRLR   DSN
Sbjct: 301 KLHNFDVHKYNRLR--SDSN 305

BLAST of Lsi01G010530 vs. NCBI nr
Match: XP_038883044.1 (calcium uniporter protein 3, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 577.8 bits (1488), Expect = 6.0e-161
Identity = 295/332 (88.86%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS+KTLARRLFNAS+RLLFRNSA  +   S NLPL+PGD GI RRFLHRRPNFPPP+TF
Sbjct: 1   MASKKTLARRLFNASSRLLFRNSAVRRTYISNNLPLNPGDFGIFRRFLHRRPNFPPPSTF 60

Query: 61  TRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEV 120
           T SLPPLPVGFGNVMEQLVSRDRILLDGLKPP PAP  VP ELEGLTVEETRKLV+LTEV
Sbjct: 61  TPSLPPLPVGFGNVMEQLVSRDRILLDGLKPPTPAPASVPAELEGLTVEETRKLVELTEV 120

Query: 121 VRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVF 180
           VRLKRKLK+IPRS ITYQEFVRICGEDCS NNGDNENYG+ELAKRLDHSGAVIVLGNFVF
Sbjct: 121 VRLKRKLKEIPRSCITYQEFVRICGEDCSGNNGDNENYGLELAKRLDHSGAVIVLGNFVF 180

Query: 181 LDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLV 240
           LDPEQM+KSIASLIPTLGIN E S  +QELEEMEKQKA+IDGKADQQARRE RWGLGFLV
Sbjct: 181 LDPEQMSKSIASLIPTLGINKEDSRASQELEEMEKQKAIIDGKADQQARRELRWGLGFLV 240

Query: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQK 300
           AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRF AKQK
Sbjct: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFTAKQK 300

Query: 301 HLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           HLMKLHNFD+HKYNRLRG  DS+ FPSSNHQF
Sbjct: 301 HLMKLHNFDVHKYNRLRGL-DSDHFPSSNHQF 331

BLAST of Lsi01G010530 vs. NCBI nr
Match: TYK12952.1 (calcium uniporter protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 541.2 bits (1393), Expect = 6.2e-150
Identity = 281/332 (84.64%), Postives = 294/332 (88.55%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS+K L RRLFNA+NRLLFRNS   +   S NLPLDPGDHGI RRFLHR+PNFPPP+TF
Sbjct: 1   MASKKALPRRLFNAANRLLFRNSPNPRPHISNNLPLDPGDHGIFRRFLHRKPNFPPPSTF 60

Query: 61  TRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEV 120
           TRS   LPVG GNVMEQL SRDRILLDGLKPP  AP  VP ELEGLTVEETRKLVKLTEV
Sbjct: 61  TRS---LPVGLGNVMEQLFSRDRILLDGLKPPTTAPASVPSELEGLTVEETRKLVKLTEV 120

Query: 121 VRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVF 180
           VRLKRKLK+IPRSWITYQEFVRICGEDC D   DNE YGVELAKRLDHSGAVIVLGN VF
Sbjct: 121 VRLKRKLKEIPRSWITYQEFVRICGEDCWD---DNEEYGVELAKRLDHSGAVIVLGNLVF 180

Query: 181 LDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLV 240
           LDPEQMAKSIASLIPTL +N+E S GN+ELEEMEKQKAMID +ADQQ RRE RWGLGFLV
Sbjct: 181 LDPEQMAKSIASLIPTLLMNDEGSEGNEELEEMEKQKAMIDVEADQQVRRELRWGLGFLV 240

Query: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQK 300
           AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQ+RF+AKQK
Sbjct: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQTRFMAKQK 300

Query: 301 HLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           HLMKLHNFDIHKYNRLRGH  SN   S+NH F
Sbjct: 301 HLMKLHNFDIHKYNRLRGH-HSNPLTSTNHHF 325

BLAST of Lsi01G010530 vs. NCBI nr
Match: XP_008440156.1 (PREDICTED: calcium uniporter protein 3, mitochondrial [Cucumis melo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 281/332 (84.64%), Postives = 293/332 (88.25%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS+K L RRLFNA+NRLLFRNS   +   S NLPLDPGDHGI RRFLHR+PNFPPP+TF
Sbjct: 1   MASKKALPRRLFNAANRLLFRNSPNPRPHISNNLPLDPGDHGIFRRFLHRKPNFPPPSTF 60

Query: 61  TRSLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEV 120
           TRS   LPVG GNVMEQL SRDRILLDGLKPP  AP  VP ELEGLTVEETRKLVKLTEV
Sbjct: 61  TRS---LPVGLGNVMEQLFSRDRILLDGLKPPTTAPASVPSELEGLTVEETRKLVKLTEV 120

Query: 121 VRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVF 180
           VRLKRKLK+IPRSWITYQEFVRICGEDC D   DNE YGVELAKRLDHSGAVIVLGN VF
Sbjct: 121 VRLKRKLKEIPRSWITYQEFVRICGEDCWD---DNEEYGVELAKRLDHSGAVIVLGNLVF 180

Query: 181 LDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLV 240
           LDPEQMAKSIASLIPTL +NNE S GN+ELEEMEKQKAMID +ADQQ RRE RWGLGFLV
Sbjct: 181 LDPEQMAKSIASLIPTLLMNNEGSEGNEELEEMEKQKAMIDVEADQQVRRELRWGLGFLV 240

Query: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQK 300
           AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQ+RF+AKQK
Sbjct: 241 AQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQTRFMAKQK 300

Query: 301 HLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           HLMKLHNFDIHKYNRLRGH  SN   S+ H F
Sbjct: 301 HLMKLHNFDIHKYNRLRGH-HSNPLTSTYHHF 325

BLAST of Lsi01G010530 vs. NCBI nr
Match: XP_004141956.1 (calcium uniporter protein 3, mitochondrial [Cucumis sativus] >KGN48444.1 hypothetical protein Csa_003893 [Cucumis sativus])

HSP 1 Score: 533.1 bits (1372), Expect = 1.7e-147
Identity = 279/334 (83.53%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADK---SINLPLDPGDHGISRRFLHRRPNFPPPATF 60
           MAS K L RRLFNA+NRLLF NS   +   S NLPLDPGDH I RRFLHRRP+FPPP+TF
Sbjct: 1   MASNKALPRRLFNAANRLLFPNSPNPRPHVSNNLPLDPGDHAIFRRFLHRRPSFPPPSTF 60

Query: 61  TR--SLPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLT 120
           TR  SLPPLPVGFGNVMEQL+SRDRILLDGLKPP      VP ELEGLTVEETRKLVKLT
Sbjct: 61  TRSSSLPPLPVGFGNVMEQLLSRDRILLDGLKPPT----SVPSELEGLTVEETRKLVKLT 120

Query: 121 EVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNF 180
           EVVRLKRKLK+IPRSWITY+EFVRICGEDCSD    NE+YGVELAKRLDHSGAVIVLGNF
Sbjct: 121 EVVRLKRKLKEIPRSWITYREFVRICGEDCSDG---NEDYGVELAKRLDHSGAVIVLGNF 180

Query: 181 VFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGF 240
           VFLDPEQMAKSIASLIP+L   NE S GN+ELEEMEKQK MID +ADQQ RRE RWGLGF
Sbjct: 181 VFLDPEQMAKSIASLIPSLVNQNEGSKGNEELEEMEKQKTMIDMEADQQVRRELRWGLGF 240

Query: 241 LVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAK 300
           LVAQTAALMRLTFWELTWDVMEPICYFITS+YFMGGYAFFLTTSKEPSFEGIYQSRF+AK
Sbjct: 241 LVAQTAALMRLTFWELTWDVMEPICYFITSSYFMGGYAFFLTTSKEPSFEGIYQSRFMAK 300

Query: 301 QKHLMKLHNFDIHKYNRLRGHDDSNQFPSSNHQF 330
           QKHLMKLHNFDIHKYNRLRG   SN F S+NH F
Sbjct: 301 QKHLMKLHNFDIHKYNRLRGLHCSNPFTSTNHHF 327

BLAST of Lsi01G010530 vs. NCBI nr
Match: KAG6604230.1 (Calcium uniporter protein 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.7e-134
Identity = 254/320 (79.38%), Postives = 280/320 (87.50%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLLFRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPATFTRS 60
           MAS KTLARRLFNASNRL F N++A+K   LPLDPGD GI RRFLH+RPNFPPP+TFTRS
Sbjct: 1   MASTKTLARRLFNASNRLFF-NTSANK---LPLDPGDQGIFRRFLHQRPNFPPPSTFTRS 60

Query: 61  LPPLPVGFGNVMEQLVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRL 120
           LP LP+GFGN MEQLVSRDRILLDGLKPP PAP   P EL+GLTVEETRKLV+L EVVR+
Sbjct: 61  LPSLPIGFGNAMEQLVSRDRILLDGLKPPAPAP--APGELDGLTVEETRKLVRLAEVVRM 120

Query: 121 KRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDP 180
           KRKLK+IP   ITY+EFVRICGED SD    +E+YGVELAKRLD SGAVI+LGN VFL P
Sbjct: 121 KRKLKEIPTGRITYREFVRICGEDNSDG---DEDYGVELAKRLDESGAVIILGNVVFLHP 180

Query: 181 EQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQT 240
           EQ+AKSI  LIPTLG+N+E     +ELEE+EK+KAMIDGKA +Q RRE RWGLGFLVAQT
Sbjct: 181 EQIAKSIGCLIPTLGMNSEDPISKKELEELEKEKAMIDGKAGRQVRRELRWGLGFLVAQT 240

Query: 241 AALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLM 300
           AALMRLTFWEL+WDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEG+YQSRF+AKQK+LM
Sbjct: 241 AALMRLTFWELSWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGLYQSRFVAKQKNLM 300

Query: 301 KLHNFDIHKYNRLRGHDDSN 321
           KLHNFD+HKYNRLR   DSN
Sbjct: 301 KLHNFDVHKYNRLR--SDSN 309

BLAST of Lsi01G010530 vs. TAIR 10
Match: AT5G66650.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 252.7 bits (644), Expect = 4.1e-67
Identity = 146/323 (45.20%), Postives = 199/323 (61.61%), Query Frame = 0

Query: 1   MASRKTLARRLFNASNRLL----FRNSAADKSINLPLDPGDHGISRRFLHRRPNFPPPAT 60
           M+S+K+L + LFN S               KS  +P D GD GI RRFLH+R  F P   
Sbjct: 1   MSSKKSLVQSLFNISKTYSRISGLTRMRPTKSGGIPPDAGDSGIRRRFLHKRAFFSPEI- 60

Query: 61  FTRSLPPLPVGFGNVMEQ-----LVSRDRILLDGLKPPMPAPGQVPDELEGLTVEETRKL 120
                  +P G GN+ME+     L + +RI LD + PP       P+    +TVE+ +KL
Sbjct: 61  -------VPKG-GNLMEKLRELTLSNNNRIRLDEMLPPPSPKKSSPEFFPAVTVEDVKKL 120

Query: 121 VKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKRLDHSGAVIV 180
           ++  E+  +K KL++I ++W+ Y EFVR+CGE  SD        G  +A  LD +G VIV
Sbjct: 121 MRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSSD-----PEQGNRVANMLDEAGNVIV 180

Query: 181 LGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKADQQARREFRW 240
           LG  V L PE++  ++A LIPTL  + +  T  QE E++E  K+ ID +AD   R+E   
Sbjct: 181 LGKLVCLKPEELTSAMAGLIPTLEPSLDAET-RQEFEQLEIIKSDIDKRADDLVRKELWA 240

Query: 241 GLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSR 300
           GLG ++AQT    RLTFWEL+WDVMEPIC+++TSTYFM GYAFFL TSKEPSFEG Y+SR
Sbjct: 241 GLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGYAFFLRTSKEPSFEGFYKSR 300

Query: 301 FIAKQKHLMKLHNFDIHKYNRLR 315
           F  KQK L+K+ +FDI ++ +L+
Sbjct: 301 FETKQKRLIKMLDFDIDRFTKLQ 308

BLAST of Lsi01G010530 vs. TAIR 10
Match: AT2G23790.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-65
Identity = 148/336 (44.05%), Postives = 202/336 (60.12%), Query Frame = 0

Query: 1   MASRKTLARRLFNASN------------------RLLFRNSAADKSINLPLDPGDHGISR 60
           MA RK L+++LFN +N                  R    N + D +   P +PGD  +SR
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAP-EPGDLAMSR 60

Query: 61  RFLHRRPNFPPPATFTRSLPPLPVGFGNVMEQL----VSRDRILLDGLKPPMPAPGQVPD 120
           RF+H      P       +  +PVG  +++E+L     S+DRI LDGL P        P+
Sbjct: 61  RFMHNSAMIRP-----AEIMQMPVG-ESLIEKLREIDGSKDRIRLDGLSP--------PE 120

Query: 121 ELEGLTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVE 180
               LTV +T+KL++  ++  +K KL++  RSW+ Y+EFV +CGE  SD      + G +
Sbjct: 121 RETSLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSD-----PDLGSK 180

Query: 181 LAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMID 240
           +AK LD S  VIVLG+ V + P+Q+ KSI  L+P   I+N       E +E+E +KA+ID
Sbjct: 181 IAKMLDDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVID 240

Query: 241 GKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTT 300
            KA    R+E   GLG+L+ QTA  MRLTFWEL+WDVMEPIC+++TS YFM GYAFFL T
Sbjct: 241 VKAHTLVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRT 300

Query: 301 SKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRLR 315
           SKEPSFEG YQSRF AKQ+ LM  + FD+ +YN L+
Sbjct: 301 SKEPSFEGFYQSRFEAKQRKLMNEYEFDLERYNELK 316

BLAST of Lsi01G010530 vs. TAIR 10
Match: AT4G36820.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 225.3 bits (573), Expect = 7.1e-59
Identity = 140/332 (42.17%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 4   RKTLARRLFN----ASNRLLFRNSAADKSI----NLPLD---------PGDHGISRRFLH 63
           +K L+ RLFN    AS  L+   +++  S+     +P D         PGD  IS+RFL+
Sbjct: 5   KKLLSNRLFNMSKTASQSLMNCRTSSSSSLAMRTRVPKDIGEATIDPEPGDLTISQRFLN 64

Query: 64  RRPNFPPPATFTRSLPPLPVGFGNVMEQL----VSRDRILLDGLKPPMPAPGQVPDELEG 123
           +   F      T S   + +G  ++ME+L    +++DRI LDGL  P        +E  G
Sbjct: 65  K---FSMNGIDTTS--KMSIG-ESLMEKLKEMDMNKDRIRLDGLSHP-------KEETLG 124

Query: 124 LTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFVRICGEDCSDNNGDNENYGVELAKR 183
           LTV++ +KL++  E+  +K KL +  + WI Y +F+ +C +   D      + G  +AK 
Sbjct: 125 LTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLD-----PSQGALIAKM 184

Query: 184 LDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAMIDGKAD 243
           LD SG VIV+GN V L P Q+ KSI  L+P   I+N      +EL E+E  K +ID KA 
Sbjct: 185 LDDSGNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAH 244

Query: 244 QQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFLTTSKEP 303
              RRE   GLG+L+ QTA  MRLTFW+LTWDVMEPIC++++S YFM GY FFL TS+EP
Sbjct: 245 SLVRRELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREP 304

Query: 304 SFEGIYQSRFIAKQKHLMKLHNFDIHKYNRLR 315
           SF+G YQSRF AKQ+ LM+  +FD+ +Y+ L+
Sbjct: 305 SFQGFYQSRFEAKQRKLMQSEDFDVGRYDELK 318

BLAST of Lsi01G010530 vs. TAIR 10
Match: AT5G42610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 198.7 bits (504), Expect = 7.1e-51
Identity = 106/235 (45.11%), Postives = 154/235 (65.53%), Query Frame = 0

Query: 79  DRILLDGLKPPMPAPGQVPDELEGLTVEETRKLVKLTEVVRLKRKLKQIPRSWITYQEFV 138
           DRI+   ++   P P +    + GL+V ET+KL++L ++ ++K +L++IP+S ++Y EF+
Sbjct: 51  DRIVGIRIESVSP-PRREETPVMGLSVSETKKLLRLYQMEKVKERLREIPKSSVSYWEFI 110

Query: 139 RICGEDCSDNNGDNENYGVELAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINN 198
           +IC E C      N+  G ++AK LDHSG V+VLG+ VFL P Q+AKS+ ++I    +  
Sbjct: 111 QICCESCG-----NDEQGSQMAKSLDHSGCVVVLGDIVFLHPHQIAKSMEAMIKQTSVLP 170

Query: 199 EVSTGNQELEEMEKQKAMIDGKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEP 258
                 +EL ++   K  ID +A +  + E   GLGFL  QT   MRLTFWEL+WDVMEP
Sbjct: 171 N-DPRKEELVQLATTKKSIDIEARRIVQAELYCGLGFLAVQTIGFMRLTFWELSWDVMEP 230

Query: 259 ICYFITSTYFMGGYAFFLTTSKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRL 314
           IC+F+T+ +F+ GY FFL TS EPSFEG+++ RF  KQK LM+ H FD  +YN L
Sbjct: 231 ICFFVTTIHFILGYIFFLRTSTEPSFEGLFRQRFKTKQKKLMERHGFDFLRYNEL 278

BLAST of Lsi01G010530 vs. TAIR 10
Match: AT1G57610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-29
Identity = 73/217 (33.64%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 98  DELEGLTVEETRKLVKLTEVVRLKRKLKQI-PRSWITYQEFVRICGEDCSDNNGDNENYG 157
           DE + +TV E +KL++L  V  +K+KL  +  +  +TY   +    +        +E + 
Sbjct: 41  DEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIE-ASQGLGIARSLDEAHA 100

Query: 158 VELAKRLDHSGAVIVLGNFVFLDPEQMAKSIASLIPTLGINNEVSTGNQELEEMEKQKAM 217
              A+ LD +G +++  + V+L P ++   I   +P LG+N +     +E ++M   K  
Sbjct: 101 --FARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVP-LGLNPDDELIREEFDKMRSMKEE 160

Query: 218 IDGKADQQARREFRWGLGFLVAQTAALMRLTFWELTWDVMEPICYFITSTYFMGGYAFFL 277
           ID  A QQ R+    GLG+ V Q    +RLTFWE +WDVMEPI +F T+T  + GYA+FL
Sbjct: 161 IDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGIIVGYAYFL 220

Query: 278 TTSKEPSFEGIYQSRFIAKQKHLMKLHNFDIHKYNRL 314
            TS++P+++   +  F+++Q+ L+K H FD  ++  L
Sbjct: 221 MTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKEL 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVR55.8e-6645.20Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O648232.4e-6444.05Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q1PE159.9e-5842.17Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9FJV71.0e-4945.11Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q8VYR06.3e-2833.64Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A5D3CNM03.0e-15084.64Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3B0G96.7e-15084.64Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103484705 PE=3 SV=1[more]
A0A0A0KI138.2e-14883.53Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G487650 PE=3 SV=1[more]
A0A6J1GCQ41.0e-13479.38Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111452996 PE=3 SV=... [more]
A0A6J1IPT82.3e-12976.88Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111478366 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883044.16.0e-16188.86calcium uniporter protein 3, mitochondrial-like [Benincasa hispida][more]
TYK12952.16.2e-15084.64calcium uniporter protein 3 [Cucumis melo var. makuwa][more]
XP_008440156.11.4e-14984.64PREDICTED: calcium uniporter protein 3, mitochondrial [Cucumis melo][more]
XP_004141956.11.7e-14783.53calcium uniporter protein 3, mitochondrial [Cucumis sativus] >KGN48444.1 hypothe... [more]
KAG6604230.11.7e-13479.38Calcium uniporter protein 2, mitochondrial, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
AT5G66650.14.1e-6745.20Protein of unknown function (DUF607) [more]
AT2G23790.11.7e-6544.05Protein of unknown function (DUF607) [more]
AT4G36820.17.1e-5942.17Protein of unknown function (DUF607) [more]
AT5G42610.17.1e-5145.11Protein of unknown function (DUF607) [more]
AT1G57610.14.5e-2933.64Protein of unknown function (DUF607) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 207..227
NoneNo IPR availablePANTHERPTHR13462:SF17CALCIUM UNIPORTER PROTEIN 4, MITOCHONDRIALcoord: 1..315
IPR006769Calcium uniporter protein, C-terminalPFAMPF04678MCUcoord: 155..313
e-value: 4.9E-48
score: 163.6
IPR039055MCU familyPANTHERPTHR13462CALCIUM UNIPORTER PROTEIN, MITOCHONDRIALcoord: 1..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G010530.1Lsi01G010530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0015292 uniporter activity