Lsi01G009460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G009460
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	PAMP-induced secreted peptide 2
Location	chr01: 7675424 .. 7675720 (+)
RNA-Seq Expression	Lsi01G009460
Synteny	Lsi01G009460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAGCATGAGGAAGATTTTAATCAGCTTGATTCTGGTGGTTGCGCTGTTGGGCAATGGCGGGTTAGTCATTGTGACAGAAGGCAGGCCATTGAACCTGATGGGCTCGGGCTCGGCGGCCGCCATCGCTGAAGATTTCTTTGAGGGGCTTTCTCTGGGAGCCATTAAGCAGTCGGGTCCGAGCCCCGGTGGCGATGGTCACAAATTCGTCAACTATGACACGTTTGGAGGTATTAAGGATTCTGGCCCCACTCCTGGCGTTGGACATAGCCACGTCACCTCCTCCCACCACTGA

mRNA sequence

ATGAGGAGCATGAGGAAGATTTTAATCAGCTTGATTCTGGTGGTTGCGCTGTTGGGCAATGGCGGGTTAGTCATTGTGACAGAAGGCAGGCCATTGAACCTGATGGGCTCGGGCTCGGCGGCCGCCATCGCTGAAGATTTCTTTGAGGGGCTTTCTCTGGGAGCCATTAAGCAGTCGGGTCCGAGCCCCGGTGGCGATGGTCACAAATTCGTCAACTATGACACGTTTGGAGGTATTAAGGATTCTGGCCCCACTCCTGGCGTTGGACATAGCCACGTCACCTCCTCCCACCACTGA

Coding sequence (CDS)

ATGAGGAGCATGAGGAAGATTTTAATCAGCTTGATTCTGGTGGTTGCGCTGTTGGGCAATGGCGGGTTAGTCATTGTGACAGAAGGCAGGCCATTGAACCTGATGGGCTCGGGCTCGGCGGCCGCCATCGCTGAAGATTTCTTTGAGGGGCTTTCTCTGGGAGCCATTAAGCAGTCGGGTCCGAGCCCCGGTGGCGATGGTCACAAATTCGTCAACTATGACACGTTTGGAGGTATTAAGGATTCTGGCCCCACTCCTGGCGTTGGACATAGCCACGTCACCTCCTCCCACCACTGA

Protein sequence

MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSHH

Homology

[Close]

BLAST of Lsi01G009460 vs. ExPASy Swiss-Prot
Match: F4JRC5 (PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 6.2e-08
Identity = 41/89 (46.07%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 4  MRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSP 63
          M K ++S IL   L+G+    ++ E RPL L  + +        F+GLSLG+IK SGPSP
Sbjct: 3  MNKNVLSSILFFMLIGS----VLVESRPLGL--TKTEEKFVASLFDGLSLGSIKDSGPSP 62

Query: 64 GGDGHKFVN-YDTFGGIKDSGPTP-GVGH 91
           G+GHK V+  DTF  +K SGP+P G GH
Sbjct: 63 -GEGHKVVDRKDTFRFVKHSGPSPSGPGH 84

[Close]

BLAST of Lsi01G009460 vs. ExPASy Swiss-Prot
Match: Q5S502 (Precursor of CEP16 OS=Arabidopsis thaliana OX=3702 GN=CEP16 PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.1e-04
Identity = 34/94 (36.17%), Postives = 46/94 (48.94%), Query Frame = 0


Query: 7   ILISLILVVALLGNGGLVIVTEGRPLNLMGSGS---AAAIAEDF------FEGLS-LGAI 66
           +++ +++V  LL   G  +    R L  M   S    + I   F       + LS L  +
Sbjct: 15  LVVLMMVVSLLLAIEGRPVKDSSRSLTQMRDSSMFNGSVIMSSFKPVESSVKDLSWLATV 74

Query: 67  KQSGPSPGGDGHKFVNYDTFGGIKDSGPTPGVGH 91
           KQSGPSPG   H+   Y  FG   DSGP+PGVGH
Sbjct: 75  KQSGPSPGVGHHRAKGYKMFGRANDSGPSPGVGH 108

[Close]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL
Match: A0A5D3CLN9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004490 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.0e-38
Identity = 86/95 (90.53%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 4  MRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSP 63
          M KI I LILVVALL NGGLVIVTE RPL LMGSGSAAAIAEDFFEGLSLGAIKQSGPS 
Sbjct: 1  MSKIFIGLILVVALLDNGGLVIVTEARPLGLMGSGSAAAIAEDFFEGLSLGAIKQSGPSA 60

Query: 64 GGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSHH 99
          GGDGHKFVNYDTFGG+KDSGPTPG GHSHVTSSHH
Sbjct: 61 GGDGHKFVNYDTFGGMKDSGPTPGDGHSHVTSSHH 95

[Close]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL
Match: A0A0A0KIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.9e-34
Identity = 80/100 (80.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 1   MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLM--GSGSAAAIAEDFFEGLSLGAIKQ 60
           MRS+  I I  ILV ALLGNGGLV VT+ RPL LM  GSGSAA IAEDFFEGLSLGAIKQ
Sbjct: 1   MRSITNIFIGFILVAALLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQ 60

Query: 61  SGPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSHH 99
           SGPS GGDGHKFVNYDT GGIKDSGP PG GH H+TSSHH
Sbjct: 61  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 100

[Close]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL
Match: A0A059AZU8 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.5e-15
Identity = 51/98 (52.04%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 1  MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIA-EDFFEGLSLGAIKQS 60
          MRS  ++L  ++LV+A+L    + +  E RP N+M + +   +A   FF+GLSLGAIKQ+
Sbjct: 1  MRSATRVL-HILLVLAML--CAVFVANEARPFNVMETKAPVGVACGGFFDGLSLGAIKQA 60

Query: 61 GPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSH 98
          GPSP G+GHKF N +T GGIKDSGP+PG GH HVT ++
Sbjct: 61 GPSP-GEGHKFTNSETLGGIKDSGPSPGEGHGHVTGTN 94

[Close]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL
Match: A0A059AY97 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H01292 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.8e-13
Identity = 53/119 (44.54%), Postives = 71/119 (59.66%), Query Frame = 0


Query: 1   MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIA-EDFFEGLSLGAIKQS 60
           MRS  ++L  ++LV+A+L    + I  E RP N+M + +   +A   FF+GLSLGAIKQ+
Sbjct: 1   MRSATRVL-HILLVLAML--SAVFIGNEARPFNVMETKAPVGVACGGFFDGLSLGAIKQA 60

Query: 61  GPSPG---------------------GDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSH 98
           GPSPG                     G+GHKF N +T GGIKDSGP+PG GH HVT ++
Sbjct: 61  GPSPGEGHRFTNSETLGGIKDSGPSPGEGHKFTNLETLGGIKDSGPSPGEGHGHVTGTN 116

[Close]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL
Match: A0A059AZV2 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02234 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.3e-13
Identity = 52/119 (43.70%), Postives = 71/119 (59.66%), Query Frame = 0


Query: 1   MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIA-EDFFEGLSLGAIKQS 60
           MRS  ++L  ++LV+A+L    + +  E RP N+M + +   +A   FF+GLSLGAIKQ+
Sbjct: 1   MRSATRVL-HILLVLAML--SAVFVGNEARPFNVMETKAPVGVACGGFFDGLSLGAIKQA 60

Query: 61  GPSPG---------------------GDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSH 98
           GPSPG                     G+GHKF N +T GGIKDSGP+PG GH HVT ++
Sbjct: 61  GPSPGEGHKFTNSETLGGIKDSGPSLGEGHKFTNLETLGGIKDSGPSPGEGHGHVTGTN 116

[Close]

BLAST of Lsi01G009460 vs. NCBI nr
Match: TYK12857.1 (hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa])

HSP 1 Score: 166.4 bits (420), Expect = 1.2e-37
Identity = 86/95 (90.53%), Postives = 87/95 (91.58%), Query Frame = 0


Query: 4  MRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSP 63
          M KI I LILVVALL NGGLVIVTE RPL LMGSGSAAAIAEDFFEGLSLGAIKQSGPS 
Sbjct: 1  MSKIFIGLILVVALLDNGGLVIVTEARPLGLMGSGSAAAIAEDFFEGLSLGAIKQSGPSA 60

Query: 64 GGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSHH 99
          GGDGHKFVNYDTFGG+KDSGPTPG GHSHVTSSHH
Sbjct: 61 GGDGHKFVNYDTFGGMKDSGPTPGDGHSHVTSSHH 95

[Close]

BLAST of Lsi01G009460 vs. NCBI nr
Match: KAE8647505.1 (hypothetical protein Csa_003084 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 1.4e-33
Identity = 80/100 (80.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 1   MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLM--GSGSAAAIAEDFFEGLSLGAIKQ 60
           MRS+  I I  ILV ALLGNGGLV VT+ RPL LM  GSGSAA IAEDFFEGLSLGAIKQ
Sbjct: 7   MRSITNIFIGFILVAALLGNGGLVTVTDARPLTLMRLGSGSAATIAEDFFEGLSLGAIKQ 66

Query: 61  SGPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSHH 99
           SGPS GGDGHKFVNYDT GGIKDSGP PG GH H+TSSHH
Sbjct: 67  SGPSAGGDGHKFVNYDTLGGIKDSGPAPGDGHRHITSSHH 106

[Close]

BLAST of Lsi01G009460 vs. NCBI nr
Match: KAG6595008.1 (PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027036.1 hypothetical protein SDJN02_11045, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 137.9 bits (346), Expect = 4.7e-29
Identity = 76/97 (78.35%), Postives = 83/97 (85.57%), Query Frame = 0


Query: 1  MRSMRKILISLILVVALL-GNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQS 60
          MR++ K+LI LILV ALL GNGGLV  TE RPLN M S   AAIAEDFFEGLSLGAIKQS
Sbjct: 1  MRNVGKLLIGLILVAALLAGNGGLV-ATEARPLNAMAS---AAIAEDFFEGLSLGAIKQS 60

Query: 61 GPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSS 97
          GPSPGGDGHKFVN+DT GGIK+SGP+PG GHSH+TSS
Sbjct: 61 GPSPGGDGHKFVNFDTLGGIKESGPSPGDGHSHITSS 93

[Close]

BLAST of Lsi01G009460 vs. NCBI nr
Match: KCW59487.1 (hypothetical protein EUGRSUZ_H02229 [Eucalyptus grandis])

HSP 1 Score: 90.1 bits (222), Expect = 1.1e-14
Identity = 51/98 (52.04%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 1  MRSMRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIA-EDFFEGLSLGAIKQS 60
          MRS  ++L  ++LV+A+L    + +  E RP N+M + +   +A   FF+GLSLGAIKQ+
Sbjct: 1  MRSATRVL-HILLVLAML--CAVFVANEARPFNVMETKAPVGVACGGFFDGLSLGAIKQA 60

Query: 61 GPSPGGDGHKFVNYDTFGGIKDSGPTPGVGHSHVTSSH 98
          GPSP G+GHKF N +T GGIKDSGP+PG GH HVT ++
Sbjct: 61 GPSP-GEGHKFTNSETLGGIKDSGPSPGEGHGHVTGTN 94

[Close]

BLAST of Lsi01G009460 vs. NCBI nr
Match: XP_030477329.1 (PAMP-induced secreted peptide 2-like isoform X2 [Syzygium oleosum])

HSP 1 Score: 88.2 bits (217), Expect = 4.3e-14
Identity = 47/85 (55.29%), Postives = 61/85 (71.76%), Query Frame = 0


Query: 13 LVVALLGNGGLVIVTEGRPLNLMGSGSAAAIA-EDFFEGLSLGAIKQSGPSPGGDGHKFV 72
          LV+A+L    + +  E RP N+M + +   +A   FF+GLSLGAIKQ+GPSP G+GHKF 
Sbjct: 12 LVLAML--SAIFVANEARPFNVMETKAPVGVACGGFFDGLSLGAIKQAGPSP-GEGHKFT 71

Query: 73 NYDTFGGIKDSGPTPGVGHSHVTSS 97
          N +T GGIKDSGP+PG GH HVT +
Sbjct: 72 NSETLGGIKDSGPSPGEGHGHVTGT 93

[Close]

BLAST of Lsi01G009460 vs. TAIR 10
Match: AT4G37290.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: endomembrane system; EXPRESSED IN: cotyledon, hypocotyl, leaf; EXPRESSED DURING: LP.04 four leaves visible; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G23270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 58.2 bits (139), Expect = 4.4e-09
Identity = 41/89 (46.07%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 4  MRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSP 63
          M K ++S IL   L+G+    ++ E RPL L  + +        F+GLSLG+IK SGPSP
Sbjct: 3  MNKNVLSSILFFMLIGS----VLVESRPLGL--TKTEEKFVASLFDGLSLGSIKDSGPSP 62

Query: 64 GGDGHKFVN-YDTFGGIKDSGPTP-GVGH 91
           G+GHK V+  DTF  +K SGP+P G GH
Sbjct: 63 -GEGHKVVDRKDTFRFVKHSGPSPSGPGH 84

[Close]

BLAST of Lsi01G009460 vs. TAIR 10
Match: AT2G23270.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: stem, sperm cell, root, stamen; EXPRESSED DURING: 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G37290.1); Has 36 Blast hits to 35 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 36; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-08
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 0


Query: 4  MRKILISLILVVALLGNGGLVIVTEGRPLNLMGSGSAAAIAEDFFEGLSLGAIKQSGPSP 63
          M K+++   L   L+ +     V E RPL L  + +   +   FF+GLSLGAIK+SGPS 
Sbjct: 3  MNKVVLGSFLFFMLVSSN---FVVEARPLGL--TKAEENLVAKFFDGLSLGAIKESGPSS 62

Query: 64 GGDGHKFVN-YDTFGGIKDSGP-TPGVGH 91
          GG+GH+FV+  +T    K SGP T G GH
Sbjct: 63 GGEGHRFVDRTETLEYGKHSGPSTSGPGH 86

[Close]

BLAST of Lsi01G009460 vs. TAIR 10
Match: AT1G49800.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: sepal, male gametophyte, stamen; EXPRESSED DURING: 4 anthesis; Has 12 Blast hits to 12 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 12; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 44.7 bits (104), Expect = 5.1e-05
Identity = 34/94 (36.17%), Postives = 46/94 (48.94%), Query Frame = 0


Query: 7   ILISLILVVALLGNGGLVIVTEGRPLNLMGSGS---AAAIAEDF------FEGLS-LGAI 66
           +++ +++V  LL   G  +    R L  M   S    + I   F       + LS L  +
Sbjct: 15  LVVLMMVVSLLLAIEGRPVKDSSRSLTQMRDSSMFNGSVIMSSFKPVESSVKDLSWLATV 74

Query: 67  KQSGPSPGGDGHKFVNYDTFGGIKDSGPTPGVGH 91
           KQSGPSPG   H+   Y  FG   DSGP+PGVGH
Sbjct: 75  KQSGPSPGVGHHRAKGYKMFGRANDSGPSPGVGH 108

The following BLAST results are available for this feature:

BLAST of Lsi01G009460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JRC5	6.2e-08	46.07	PAMP-induced secreted peptide 2 OS=Arabidopsis thaliana OX=3702 GN=PIP2 PE=1 SV=...	[more]
Q5S502	7.1e-04	36.17	Precursor of CEP16 OS=Arabidopsis thaliana OX=3702 GN=CEP16 PE=3 SV=1	[more]

BLAST of Lsi01G009460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CLN9	6.0e-38	90.53	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A0A0KIE2	6.9e-34	80.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491710 PE=4 SV=1	[more]
A0A059AZU8	5.5e-15	52.04	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02229 PE=4 SV...	[more]
A0A059AY97	1.8e-13	44.54	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H01292 PE=4 SV...	[more]
A0A059AZV2	2.3e-13	43.70	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02234 PE=4 SV...	[more]

BLAST of Lsi01G009460 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK12857.1	1.2e-37	90.53	hypothetical protein E5676_scaffold255G004490 [Cucumis melo var. makuwa]	[more]
KAE8647505.1	1.4e-33	80.00	hypothetical protein Csa_003084 [Cucumis sativus]	[more]
KAG6595008.1	4.7e-29	78.35	PAMP-induced secreted peptide 2, partial [Cucurbita argyrosperma subsp. sororia]...	[more]
KCW59487.1	1.1e-14	52.04	hypothetical protein EUGRSUZ_H02229 [Eucalyptus grandis]	[more]
XP_030477329.1	4.3e-14	55.29	PAMP-induced secreted peptide 2-like isoform X2 [Syzygium oleosum]	[more]

BLAST of Lsi01G009460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37290.1	4.4e-09	46.07	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response...	[more]
AT2G23270.1	1.7e-08	43.82	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G49800.1	5.1e-05	36.17	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044700	PIP2/PIPL1	PANTHER	PTHR34663	OS06G0637400 PROTEIN	coord: 5..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G009460.1	Lsi01G009460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045087	innate immune response
biological_process	GO:0050793	regulation of developmental process