Homology
BLAST of Lsi01G007670 vs. ExPASy Swiss-Prot
Match:
Q9LV77 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 GN=ASN2 PE=2 SV=1)
HSP 1 Score: 993.4 bits (2567), Expect = 1.1e-288
Identity = 480/569 (84.36%), Postives = 520/569 (91.39%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61 QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFSFH-KSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +++ S P V+ A KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVAAVA RHL SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FT
Sbjct: 241 LDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYF
Sbjct: 301 VQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH +DCLRANKSTSAWG+EARVPFLDKEF+++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
+I+PDLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D
Sbjct: 421 LIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
ML NA+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAKAVEWDATW
Sbjct: 481 TMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIAK 568
SKNLDPSGRAALGVHVAAY+E A AK
Sbjct: 541 SKNLDPSGRAALGVHVAAYEEDKAAAAAK 564
BLAST of Lsi01G007670 vs. ExPASy Swiss-Prot
Match:
Q9LFU1 (Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 GN=ASN3 PE=2 SV=1)
HSP 1 Score: 990.7 bits (2560), Expect = 7.4e-288
Identity = 480/561 (85.56%), Postives = 513/561 (91.44%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61 QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFS-FHKSNVMSPTVV-ILAHKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +FS S P VV KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVA+VA RHL SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFT
Sbjct: 241 LDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYH+ET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH YDCLRANKSTSAWG+EARVPFLDKEFI++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MIRPDLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++
Sbjct: 421 MIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSE 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
MLMNA+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDA W
Sbjct: 481 TMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDE 560
S+NLDPSGRAALGVHV+AY E
Sbjct: 541 SQNLDPSGRAALGVHVSAYGE 556
BLAST of Lsi01G007670 vs. ExPASy Swiss-Prot
Match:
Q43011 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0265000 PE=2 SV=3)
HSP 1 Score: 969.1 bits (2504), Expect = 2.3e-281
Identity = 467/573 (81.50%), Postives = 514/573 (89.70%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLG D S AKRSRIIELSRRLRHRGPDWSG+HC+++CYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGVADVSLAKRSRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDK+VVVTVNGEIYNH+EL+ LK H+F+T SDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKSVVVTVNGEIYNHEELKANLKSHKFQTASDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGI PLYMGWGLDGS+WF+SEMKALSDDCERF+SF PGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGICPLYMGWGLDGSVWFSSEMKALSDDCERFISFPPGHLY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFSFH-KSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSK G L R N +FS S +P ++ + KA+ KRLMTDVPFGVLLSGG
Sbjct: 181 SSKTG-----GLRRWYNPPWFSESIPSTPYNPLLLRQSFEKAIIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVA+V SRHLA+++ A QWG++LHTFC+GLKGSPDL AA+EVADYLGT HHE HFT
Sbjct: 241 LDSSLVASVVSRHLAEAKVAAQWGNKLHTFCIGLKGSPDLRAAKEVADYLGTVHHELHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQEGIDALEEVIYH+ET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQEGIDALEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWG+EARVPFLDK FI++AM IDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVEARVPFLDKNFINVAMDIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MI+ DLGRIEKWVLRNAFDDE++PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA + V+D
Sbjct: 421 MIKRDLGRIEKWVLRNAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANEHVSD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
+M+MNA+F+YPENTP TKEAYYYRTIFEKFFPKNAAR TVPGGPSVACSTAKAVEWDA W
Sbjct: 481 SMMMNASFVYPENTPVTKEAYYYRTIFEKFFPKNAARLTVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIAKRSTI 572
SKNLDPSGRAALGVH AAY++ L A + +
Sbjct: 541 SKNLDPSGRAALGVHDAAYEDTLQKSPASANPV 568
BLAST of Lsi01G007670 vs. ExPASy Swiss-Prot
Match:
P31752 (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 PE=2 SV=2)
HSP 1 Score: 907.1 bits (2343), Expect = 1.1e-262
Identity = 436/569 (76.63%), Postives = 494/569 (86.82%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC D SQAKR R++ELSRRL+HRGPDWSGL +C+L+HQRLAI+DP SGD
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDK++VVTVNGEIYNH+ELR +L H++RTGSDCEVIAHLYEE+GE+FVDMLDGM
Sbjct: 61 QPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR F+AARDA+GITPLY+GWGLDGS+W +SEMK L+DDCE F F PG++Y
Sbjct: 121 FSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILAHK-AVTKRLMTDVPFGVLLSG 240
SS+ G ++ W N T S P V+ A + AV KRLMTDVPFGVLLSG
Sbjct: 181 SSRSGSFRRWYNPQWY--NETI----PSAPYDPLVLRKAFEDAVIKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAV +RHLA S+AA QWG+QLH+FCVGL+GSPDL AA+EVA+YLGT HHEFHF
Sbjct: 241 GLDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEVAEYLGTVHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQ+GIDA+E+VI+HIET+DVTTIRASTPMFLM+RKIKSLGVKMV+SGEGSDEIFGGYLY
Sbjct: 301 TVQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNK+EFH ETCRKIKALH YDCLRANK+TSAWGLEARVPFLDKEF+ +AMSIDPE
Sbjct: 361 FHKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFMDVAMSIDPES 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMI+PDLGRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLK HA K VT
Sbjct: 421 KMIKPDLGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAAKHVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
D M++NA IYP NTPTTKEAYYYR IFE+FFP+N+AR TVPGGPS+ACSTAKA+EWDA
Sbjct: 481 DRMMLNAARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPGGPSIACSTAKAIEWDAR 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WS NLDPSGRAALGVH +AYD L + I+
Sbjct: 541 WSNNLDPSGRAALGVHDSAYDPPLPSSIS 563
BLAST of Lsi01G007670 vs. ExPASy Swiss-Prot
Match:
P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)
HSP 1 Score: 897.5 bits (2318), Expect = 8.5e-260
Identity = 432/564 (76.60%), Postives = 491/564 (87.06%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC D +QAKR R++ELSRRL+HRGPDWSGLH +CYLAHQRLAIVDP SGD
Sbjct: 1 MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPL+NEDK+++VTVNGEIYNH+ELR++L HQFRTGSDC+VIAHLYEE+GE F+DMLDG+
Sbjct: 61 QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGI 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR TFI ARDAIG+T LY+GWGLDGS+W +SEMK L+DDCE F F PGH+Y
Sbjct: 121 FSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFSFH-KSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SS++ R N T+FS S P V A KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSRE-----RAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVA++ SR+LA ++AA QWGS+LH+FCVGL+GSPDL AA+EVADYLGT HHEF FT
Sbjct: 241 LDSSLVASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYH+ET+DVTTIRASTPMFLMSRKIKSLGVK V+SGEGSDEIFGGYLYF
Sbjct: 301 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNK+EFH ETCRKIKALH YDCLRANKST AWGLEARVPFLDKEFI++AM+IDPE+K
Sbjct: 361 HKAPNKEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MI+ D GRIEK++LR AFDDE++PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K VTD
Sbjct: 421 MIKRDEGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVTD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
M++NA I+ NTP TKEAYYYR IFE+FFP+N+AR TVPGGP+VACSTAKAVEWDA W
Sbjct: 481 KMILNAGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLN 563
S NLDPSGRAALGVH++AYD+K N
Sbjct: 541 SNNLDPSGRAALGVHLSAYDDKQN 559
BLAST of Lsi01G007670 vs. ExPASy TrEMBL
Match:
A0A0A0KG93 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G500420 PE=4 SV=1)
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 532/568 (93.66%), Postives = 541/568 (95.25%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFS-FHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSKQG EL R N +F+ S P V+ A KAVTKRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----ELRRWYNPPWFTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDL AAREVADYLGTRHHEFHFT
Sbjct: 241 LDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLSAAREVADYLGTRHHEFHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFI+IAMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD
Sbjct: 421 MIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
AML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA W
Sbjct: 481 AMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIA 567
SKNLDPSGRAALGVHVAAYDEKLNAKIA
Sbjct: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIA 563
BLAST of Lsi01G007670 vs. ExPASy TrEMBL
Match:
A0A5D3CQI6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002840 PE=4 SV=1)
HSP 1 Score: 1072.8 bits (2773), Expect = 4.6e-310
Identity = 527/569 (92.62%), Postives = 538/569 (94.55%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W F S P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPPW------FTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFI+IAMSIDP+W
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPDW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
D+ML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAYDEKLN KIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYDEKLNGKIA 563
BLAST of Lsi01G007670 vs. ExPASy TrEMBL
Match:
A0A1S3B235 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103484971 PE=4 SV=1)
HSP 1 Score: 1072.8 bits (2773), Expect = 4.6e-310
Identity = 527/569 (92.62%), Postives = 538/569 (94.55%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W F S P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPPW------FTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFI+IAMSIDP+W
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPDW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
D+ML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAYDEKLN KIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYDEKLNGKIA 563
BLAST of Lsi01G007670 vs. ExPASy TrEMBL
Match:
A0A5A7SYK6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G001010 PE=4 SV=1)
HSP 1 Score: 1066.6 bits (2757), Expect = 3.9e-308
Identity = 526/581 (90.53%), Postives = 542/581 (93.29%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG-------MHLFSELWRIGNSTFF----SFHKSN----VMSPTVVILAHKAVTKRL 240
SSKQG F+E+ G+ +F K++ + + L AVTKRL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKASSGLLIFDLSHYCLLQGAVTKRL 240
Query: 241 MTDVPFGVLLSGGLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
MTDVPFGVLLSGGLDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA
Sbjct: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
Query: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
DYLGTRHHEFHFTVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSG
Sbjct: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
Query: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420
EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE
Sbjct: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420
Query: 421 FISIAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
FI+IAMSIDP+WKMIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI
Sbjct: 421 FINIAMSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
Query: 481 DGLKDHAEKQVTDAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
DGLKDHAEKQVTD+ML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA
Sbjct: 481 DGLKDHAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
Query: 541 CSTAKAVEWDATWSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
CSTAKAVEWDA WSKNLDPSGRAALGVHVAAYDEKLN KIA
Sbjct: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIA 581
BLAST of Lsi01G007670 vs. ExPASy TrEMBL
Match:
A0A6J1IT53 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC111478427 PE=4 SV=1)
HSP 1 Score: 1065.8 bits (2755), Expect = 6.7e-308
Identity = 523/569 (91.92%), Postives = 534/569 (93.85%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVL CVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENC+LAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKT+VVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W + P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPQW------YTEMVPLGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVA RHLADSEAA QWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWG+E RVPFLDKEFIS AMSIDP+W
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVETRVPFLDKEFISTAMSIDPDW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
DAMLMNANFIYPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAY++KLNAKIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYEQKLNAKIA 563
BLAST of Lsi01G007670 vs. NCBI nr
Match:
XP_031743076.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis sativus] >KGN48750.1 hypothetical protein Csa_003204 [Cucumis sativus])
HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 532/568 (93.66%), Postives = 541/568 (95.25%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFS-FHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSKQG EL R N +F+ S P V+ A KAVTKRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----ELRRWYNPPWFTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDL AAREVADYLGTRHHEFHFT
Sbjct: 241 LDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLSAAREVADYLGTRHHEFHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFI+IAMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD
Sbjct: 421 MIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
AML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA W
Sbjct: 481 AMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIA 567
SKNLDPSGRAALGVHVAAYDEKLNAKIA
Sbjct: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIA 563
BLAST of Lsi01G007670 vs. NCBI nr
Match:
XP_038882037.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Benincasa hispida])
HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 530/569 (93.15%), Postives = 539/569 (94.73%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGH+FRTGSDCEVIAHLYEEYGE+FVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHEFRTGSDCEVIAHLYEEYGEDFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W + S P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPPW------YTEMVPSGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVASRHL DSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVASRHLVDSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
DAMLMNA+FIYPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DAMLMNASFIYPENTPMTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAYDEKLNAKIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 563
BLAST of Lsi01G007670 vs. NCBI nr
Match:
XP_008440595.1 (PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis melo] >TYK12696.1 asparagine synthetase [Cucumis melo var. makuwa])
HSP 1 Score: 1072.8 bits (2773), Expect = 9.6e-310
Identity = 527/569 (92.62%), Postives = 538/569 (94.55%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W F S P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPPW------FTEMVPSGSYDPLVLRNAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFI+IAMSIDP+W
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFINIAMSIDPDW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
D+ML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAYDEKLN KIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYDEKLNGKIA 563
BLAST of Lsi01G007670 vs. NCBI nr
Match:
KAA0036302.1 (asparagine synthetase [Cucumis melo var. makuwa])
HSP 1 Score: 1066.6 bits (2757), Expect = 8.1e-308
Identity = 526/581 (90.53%), Postives = 542/581 (93.29%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR+KTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRSKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG-------MHLFSELWRIGNSTFF----SFHKSN----VMSPTVVILAHKAVTKRL 240
SSKQG F+E+ G+ +F K++ + + L AVTKRL
Sbjct: 181 SSKQGGLRRWYNPPWFTEMVPSGSYDPLVLRNAFEKASSGLLIFDLSHYCLLQGAVTKRL 240
Query: 241 MTDVPFGVLLSGGLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
MTDVPFGVLLSGGLDSSLVAAVA RHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA
Sbjct: 241 MTDVPFGVLLSGGLDSSLVAAVACRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVA 300
Query: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
DYLGTRHHEFHFTVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSG
Sbjct: 301 DYLGTRHHEFHFTVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSG 360
Query: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420
EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE
Sbjct: 361 EGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKE 420
Query: 421 FISIAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
FI+IAMSIDP+WKMIR D+GRIEKW+LRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI
Sbjct: 421 FINIAMSIDPDWKMIRRDIGRIEKWILRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWI 480
Query: 481 DGLKDHAEKQVTDAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
DGLKDHAEKQVTD+ML+NANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA
Sbjct: 481 DGLKDHAEKQVTDSMLVNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVA 540
Query: 541 CSTAKAVEWDATWSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
CSTAKAVEWDA WSKNLDPSGRAALGVHVAAYDEKLN KIA
Sbjct: 541 CSTAKAVEWDAAWSKNLDPSGRAALGVHVAAYDEKLNGKIA 581
BLAST of Lsi01G007670 vs. NCBI nr
Match:
XP_022978448.1 (asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita maxima])
HSP 1 Score: 1065.8 bits (2755), Expect = 1.4e-307
Identity = 523/569 (91.92%), Postives = 534/569 (93.85%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVL CVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENC+LAHQRLAIVDPTSGD
Sbjct: 1 MCGILAVLSCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCFLAHQRLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKT+VVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM
Sbjct: 61 QPLYNEDKTIVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY
Sbjct: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSKQG ++ W + P V+ A KAVTKRLMTDVPFGVLLSG
Sbjct: 181 SSKQGGLRRWYNPQW------YTEMVPLGHYDPLVLRKAFEKAVTKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVAAVA RHLADSEAA QWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF
Sbjct: 241 GLDSSLVAAVACRHLADSEAANQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
TVQEGIDALEEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY
Sbjct: 301 TVQEGIDALEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWG+E RVPFLDKEFIS AMSIDP+W
Sbjct: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGVETRVPFLDKEFISTAMSIDPDW 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT
Sbjct: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
DAMLMNANFIYPENTP TKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDA
Sbjct: 481 DAMLMNANFIYPENTPATKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAA 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIA 567
WSKNLDPSGRAALGVHVAAY++KLNAKIA
Sbjct: 541 WSKNLDPSGRAALGVHVAAYEQKLNAKIA 563
BLAST of Lsi01G007670 vs. TAIR 10
Match:
AT5G65010.1 (asparagine synthetase 2 )
HSP 1 Score: 993.4 bits (2567), Expect = 8.1e-290
Identity = 480/569 (84.36%), Postives = 520/569 (91.39%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61 QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFSFH-KSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +++ S P V+ A KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVAAVA RHL SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FT
Sbjct: 241 LDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYF
Sbjct: 301 VQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH +DCLRANKSTSAWG+EARVPFLDKEF+++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
+I+PDLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D
Sbjct: 421 LIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
ML NA+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPS+ACSTAKAVEWDATW
Sbjct: 481 TMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSIACSTAKAVEWDATW 540
Query: 541 SKNLDPSGRAALGVHVAAYDEKLNAKIAK 568
SKNLDPSGRAALGVHVAAY+E A AK
Sbjct: 541 SKNLDPSGRAALGVHVAAYEEDKAAAAAK 564
BLAST of Lsi01G007670 vs. TAIR 10
Match:
AT5G10240.1 (asparagine synthetase 3 )
HSP 1 Score: 990.7 bits (2560), Expect = 5.3e-289
Identity = 480/561 (85.56%), Postives = 513/561 (91.44%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHLYEE+GEEFVDMLDGM
Sbjct: 61 QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFS-FHKSNVMSPTVV-ILAHKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +FS S P VV KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVA+VA RHL SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFT
Sbjct: 241 LDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYH+ET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH YDCLRANKSTSAWG+EARVPFLDKEFI++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MIRPDLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++
Sbjct: 421 MIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSE 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
MLMNA+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDA W
Sbjct: 481 TMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDE 560
S+NLDPSGRAALGVHV+AY E
Sbjct: 541 SQNLDPSGRAALGVHVSAYGE 556
BLAST of Lsi01G007670 vs. TAIR 10
Match:
AT5G65010.2 (asparagine synthetase 2 )
HSP 1 Score: 988.8 bits (2555), Expect = 2.0e-288
Identity = 480/570 (84.21%), Postives = 520/570 (91.23%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC+D+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAI+DPTSGD
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKTV VTVNGEIYNHK LREKLK HQFRTGSDCEVIAHLYEE+GEEF+DMLDGM
Sbjct: 61 QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+SF PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFSFH-KSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +++ S P V+ A KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVAAVA RHL SEAARQWGSQLHTFC+GL+GSPDL A REVADYLGTRHHEF FT
Sbjct: 241 LDSSLVAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYHIET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEI GGYLYF
Sbjct: 301 VQDGIDAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH +DCLRANKSTSAWG+EARVPFLDKEF+++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
+I+PDLGRIEKWVLRNAFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V+D
Sbjct: 421 LIKPDLGRIEKWVLRNAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSD 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPK-NAARSTVPGGPSVACSTAKAVEWDAT 540
ML NA+F++P+NTP TKEAYYYRTIFEKFFPK +AAR+TVPGGPS+ACSTAKAVEWDAT
Sbjct: 481 TMLSNASFVFPDNTPLTKEAYYYRTIFEKFFPKQSAARATVPGGPSIACSTAKAVEWDAT 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEKLNAKIAK 568
WSKNLDPSGRAALGVHVAAY+E A AK
Sbjct: 541 WSKNLDPSGRAALGVHVAAYEEDKAAAAAK 565
BLAST of Lsi01G007670 vs. TAIR 10
Match:
AT5G10240.2 (asparagine synthetase 3 )
HSP 1 Score: 979.5 bits (2531), Expect = 1.2e-285
Identity = 477/561 (85.03%), Postives = 511/561 (91.09%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGCVD+SQAKRSRIIELSRRLRHRGPDWSGLHC+E+CYLAH+RLAIVDPTSGD
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPLYNEDKT+ VTVNGEIYNHK LRE LK HQFRTGSDCEVIAHL ++GEEFVDMLDGM
Sbjct: 61 QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
F+FVLLDTR K+FIAARDAIGITPLY+GWGLDGS+WFASEMKALSDDCE+F+ F PGHIY
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180
Query: 181 SSKQGMHLFSELWRIGNSTFFS-FHKSNVMSPTVV-ILAHKAVTKRLMTDVPFGVLLSGG 240
SSKQG L R N +FS S P VV KAV KRLMTDVPFGVLLSGG
Sbjct: 181 SSKQG-----GLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGG 240
Query: 241 LDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHFT 300
LDSSLVA+VA RHL SEAA QWGS+LHTFC+GLKGSPDL A REVADYLGTRHHE HFT
Sbjct: 241 LDSSLVASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFT 300
Query: 301 VQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
VQ+GIDA+EEVIYH+ET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF
Sbjct: 301 VQDGIDAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 360
Query: 361 HKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEWK 420
HKAPNKKEFHEETCRKIKALH YDCLRANKSTSAWG+EARVPFLDKEFI++AMSIDPEWK
Sbjct: 361 HKAPNKKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWK 420
Query: 421 MIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVTD 480
MIRPDLGRIEKWVLRNAFDDEK PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA K V++
Sbjct: 421 MIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKDHANKHVSE 480
Query: 481 AMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDATW 540
MLMNA+F++P+NTP TKEAYYYRTIFEKFFPK+AAR+TVPGGPSVACSTAKAVEWDA W
Sbjct: 481 TMLMNASFVFPDNTPLTKEAYYYRTIFEKFFPKSAARATVPGGPSVACSTAKAVEWDAAW 540
Query: 541 SKNLDPSGRAALGVHVAAYDE 560
S+NLDPSGRAALGVHV+AY E
Sbjct: 541 SQNLDPSGRAALGVHVSAYGE 555
BLAST of Lsi01G007670 vs. TAIR 10
Match:
AT3G47340.1 (glutamine-dependent asparagine synthase 1 )
HSP 1 Score: 889.0 bits (2296), Expect = 2.2e-258
Identity = 424/563 (75.31%), Postives = 486/563 (86.32%), Query Frame = 0
Query: 1 MCGILAVLGCVDSSQAKRSRIIELSRRLRHRGPDWSGLHCFENCYLAHQRLAIVDPTSGD 60
MCGILAVLGC D SQAKR R++ELSRRLRHRGPDWSGL+ + YLAHQRLA++DP SGD
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60
Query: 61 QPLYNEDKTVVVTVNGEIYNHKELREKLKGHQFRTGSDCEVIAHLYEEYGEEFVDMLDGM 120
QPL+NEDKT+VVTVNGEIYNH+ELR++LK H+FRTGSDCEVIAHLYEEYG +FVDMLDG+
Sbjct: 61 QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120
Query: 121 FSFVLLDTRTKTFIAARDAIGITPLYMGWGLDGSIWFASEMKALSDDCERFVSFLPGHIY 180
FSFVLLDTR +F+ ARDAIG+T LY+GWGLDGS+W +SEMK L+DDCE F +F PGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180
Query: 181 SSKQG--MHLFSELWRIGNSTFFSFHKSNVMSPTVVILA-HKAVTKRLMTDVPFGVLLSG 240
SSK G ++ W F S P + A AV KRLMTDVPFGVLLSG
Sbjct: 181 SSKLGGFKQWYNPPW------FNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSG 240
Query: 241 GLDSSLVAAVASRHLADSEAARQWGSQLHTFCVGLKGSPDLGAAREVADYLGTRHHEFHF 300
GLDSSLVA++ +RHLA ++AA+QWG QLH+FCVGL+GSPDL A +EVA+YLGT HHEFHF
Sbjct: 241 GLDSSLVASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHF 300
Query: 301 TVQEGIDALEEVIYHIETFDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLY 360
+VQ+GIDA+E+VIYH+ET+DVTTIRASTPMFLMSRKIKSLGVKMVLSGEG+DEIFGGYLY
Sbjct: 301 SVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLY 360
Query: 361 FHKAPNKKEFHEETCRKIKALHLYDCLRANKSTSAWGLEARVPFLDKEFISIAMSIDPEW 420
FHKAPNKKEFH+ETCRKIKALH YDCLRANKSTSA+GLEARVPFLDK+FI+ AMS+DPE
Sbjct: 361 FHKAPNKKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPES 420
Query: 421 KMIRPDLGRIEKWVLRNAFDDEKEPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAEKQVT 480
KMI+P+ GRIEKWVLR AFDDE+ PYLPKHILYRQKEQFSDGVGYSWIDGLKDHA + V
Sbjct: 421 KMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAQNVN 480
Query: 481 DAMLMNANFIYPENTPTTKEAYYYRTIFEKFFPKNAARSTVPGGPSVACSTAKAVEWDAT 540
D M+ NA I+P NTP TKEAYYYR IFE+FFP+N+AR TVPGG +VACSTAKAVEWDA+
Sbjct: 481 DKMMSNAGHIFPHNTPNTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAKAVEWDAS 540
Query: 541 WSKNLDPSGRAALGVHVAAYDEK 561
WS N+DPSGRAA+GVH++AYD K
Sbjct: 541 WSNNMDPSGRAAIGVHLSAYDGK 557
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LV77 | 1.1e-288 | 84.36 | Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Arabidopsis thaliana OX=3702 ... | [more] |
Q9LFU1 | 7.4e-288 | 85.56 | Asparagine synthetase [glutamine-hydrolyzing] 3 OS=Arabidopsis thaliana OX=3702 ... | [more] |
Q43011 | 2.3e-281 | 81.50 | Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... | [more] |
P31752 | 1.1e-262 | 76.63 | Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis OX=4686 P... | [more] |
P49092 | 8.5e-260 | 76.60 | Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=A... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KG93 | 0.0e+00 | 93.66 | Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_... | [more] |
A0A5D3CQI6 | 4.6e-310 | 92.62 | Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... | [more] |
A0A1S3B235 | 4.6e-310 | 92.62 | Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1034... | [more] |
A0A5A7SYK6 | 3.9e-308 | 90.53 | Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119... | [more] |
A0A6J1IT53 | 6.7e-308 | 91.92 | Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita maxima OX=3661 GN=LOC... | [more] |
Match Name | E-value | Identity | Description | |
XP_031743076.1 | 0.0e+00 | 93.66 | asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis sativus] >KGN48750.1 hy... | [more] |
XP_038882037.1 | 0.0e+00 | 93.15 | asparagine synthetase [glutamine-hydrolyzing] 2 [Benincasa hispida] | [more] |
XP_008440595.1 | 9.6e-310 | 92.62 | PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 2 [Cucumis melo] >TYK12... | [more] |
KAA0036302.1 | 8.1e-308 | 90.53 | asparagine synthetase [Cucumis melo var. makuwa] | [more] |
XP_022978448.1 | 1.4e-307 | 91.92 | asparagine synthetase [glutamine-hydrolyzing] 2 [Cucurbita maxima] | [more] |