Lsi01G006590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G006590
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationchr01: 5306805 .. 5309135 (+)
RNA-Seq ExpressionLsi01G006590
SyntenyLsi01G006590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTTTTAAAAATACTCCATATGAATTCAGGAGTTGGAGAAACAAGCTATGCCAAAAACTCCTTACTTCAGGTCTCTCTTACTTTTATAAACTCACTCACTTCCCTTTTTCTACTCTTTTTAATTTTTCTTTTTTAACATATATTTGTGTTCTTTAATTTCATCACATCATTATTATTATTAAAGAAAATTATGGCTTAATTATATTTTCATTTATTGTATTTTTTTCTGCTATTTTTTTTTTTGTTTAAGTTATTTATTATAGAATTCTTTGTATTCTAATTAAGTTAAATGATTTAATAATATAATGTACAAAATTCACAATCATTATCCCAAACAAATTGTTTATGCAAAGTTGGTCATGATTATGTTGTTTTTTATTATTTTTTTTCCTTATTTGATGTTAGTTTTGCTTCAATATTCTTTCTTTTTTGTCTTTCGTTTTATTGTACAGTATCTTTTATCTTTCTTAACTACAAATTTAGTACCTAAACTTTAAAGGTTTTGTGTATATTTGGTCTTGAACTTTAAAAAATATAAATTTAATCCTTTAGGCTTTTGAAATTTTTTAAAATTCACAAATCCATTAAATACAAAATTAAATGCATTTATAGTTTCATTTAAAAATATAATCAAAATTTTATATCTAATTGTTGGGCTCACAGAAGCAATCTCTTCAAAAACTACAACTCTTCTACTATTGATTTAAATCTAAGTACTTTTTGTAACAATTAATCGCGCTATATTGACTTATCTCTAGCCTTGCTGCGTTTTTGTACTCTCCTTGGTGCTTAATGAATATATTTGAATGATAGATGATGCTGGTGCTAAGAAGGTGTCCACCTAGTGGAGATGTTCCGGTGCATCCCTTATCCTCTGTATCCTTTTCAAAAAAAAAAAAAATTTATATCTAATCGATTAAATTAATTTTTAAAATTTATGAATTTTTTCATGGCCTATTAGACACAAATTAAACAGAAAGTCTACAAAACTATTAGATACAAAATTTAAAGTTTAGAAACTATTAGACACTTTTAAAATTCAAAAACCAAAAAATATAAAGTATAAATTTCATGGACTAAACTTGTAATTTAACTTGTATGGTTGTTATTATTATTATAGATATTTATTTATTTATTTATTTATGATATTGATGATGTGATTAGCAAAAGGTAATATCCTTAGCGTGGCCAATCATAAAGGAAGCTCTTGAAGACTTCTGCAGTGCTCAAAACATTCCAACCACTTTAAAAATAGCAGATTTGGGATGTTCTTCAGGACCAAACACTCTTATGATTGTCTCAAATTTAATCAAACAATTTCATAAAAATCTTCACAATAAATCATTCCAATATCAAATCTTCTCCAACGATCTCCCTGCAAACGATTTTAATGCCATTTTTAGATCCTTGCCAAATTTCTTGGAAGATTTGAAGAACCAAATTGGAGCTGATTTTGGGAGCTGTTTCTTCAATGGGGTGCCGGGTTCTTTTTATGGCAGGCTTTTCCCCAACAAAAGTTTGCATTTTGTTCATTCTTCTTACAGTCTTCAATGGCTCTCTCAGGTGATCTTTAAAATCTTATTCTCTACAAAAATAAAAATCCATCAATTACAATATATTATTATTAACTTTATGGAAATTCAATTATTAATGTATATAGGTTCCAAAAGGGATGGAGATAAAAAACAAAGGGAACATATTCATAGACAGTACAAGTCCAGAGAATGTGATTGAAGGGTATTATAGGCAATTTCAAAAGGATTTTTCACTGTTTTTGAAGTGTCGTGGAGAAGAAGTAGTAACTGGTGGACGCATGGTTCTTACCTTTGTAGGAAGAACAGATGAAAGATTATTGTTATGCTCTTAAATTTTTGAATTTAGCTCTCAATAACATGGTTGCAGAGGTGAAAATACAATATATATAATTAATACCCCTCTAATTAAATATTGTATTTTTGTTTCATTGAGAAAATTTTCTAAACACAATCATTTAATATAATTTCTTTATGGTGGTGAATCATCACAGGGGATCGTTAAAGAGGAGAAAGTTGATAGATTCAATTTTCCAAACTTCATGCCAAGTCCCGAGGAAGTAAAAGCAGAGGTTTTAAAAGAAGGAAGCTTCATCATAAATCGTCTCGAAGTCTCAAGAATTGATTGGAATTTCTACAATACTGAATTTGATGGATCAAATGTGTTTGTTGATAGCAGTTATCATTTTGTAAAATACATGAGATCTGTGCTTCAACCACTTTTGATTTCTCATTTTGGAGAAGTCATTGTAGAAGAATTATTTAAGAGGTACAGACAAATTGTTAAGGATGAG

mRNA sequence

ATGGAGGTAATATCCTTAGCGTGGCCAATCATAAAGGAAGCTCTTGAAGACTTCTGCAGTGCTCAAAACATTCCAACCACTTTAAAAATAGCAGATTTGGGATGTTCTTCAGGACCAAACACTCTTATGATTGTCTCAAATTTAATCAAACAATTTCATAAAAATCTTCACAATAAATCATTCCAATATCAAATCTTCTCCAACGATCTCCCTGCAAACGATTTTAATGCCATTTTTAGATCCTTGCCAAATTTCTTGGAAGATTTGAAGAACCAAATTGGAGCTGATTTTGGGAGCTGTTTCTTCAATGGGGTGCCGGGTTCTTTTTATGGCAGGCTTTTCCCCAACAAAAGTTTGCATTTTGTTCATTCTTCTTACAGTCTTCAATGGCTCTCTCAGGTTCCAAAAGGGATGGAGATAAAAAACAAAGGGAACATATTCATAGACAGTACAAGTCCAGAGAATGTGATTGAAGGGTATTATAGGCAATTTCAAAAGGATTTTTCACTGTTTTTGAAGTGTCGTGGAGAAGAAGTAGTAACTGGTGGACGCATGGGGATCGTTAAAGAGGAGAAAGTTGATAGATTCAATTTTCCAAACTTCATGCCAAGTCCCGAGGAAGTAAAAGCAGAGGTTTTAAAAGAAGGAAGCTTCATCATAAATCGTCTCGAAGTCTCAAGAATTGATTGGAATTTCTACAATACTGAATTTGATGGATCAAATGTGTTTGTTGATAGCAGTTATCATTTTGTAAAATACATGAGATCTGTGCTTCAACCACTTTTGATTTCTCATTTTGGAGAAGTCATTGTAGAAGAATTATTTAAGAGGTACAGACAAATTGTTAAGGATGAG

Coding sequence (CDS)

ATGGAGGTAATATCCTTAGCGTGGCCAATCATAAAGGAAGCTCTTGAAGACTTCTGCAGTGCTCAAAACATTCCAACCACTTTAAAAATAGCAGATTTGGGATGTTCTTCAGGACCAAACACTCTTATGATTGTCTCAAATTTAATCAAACAATTTCATAAAAATCTTCACAATAAATCATTCCAATATCAAATCTTCTCCAACGATCTCCCTGCAAACGATTTTAATGCCATTTTTAGATCCTTGCCAAATTTCTTGGAAGATTTGAAGAACCAAATTGGAGCTGATTTTGGGAGCTGTTTCTTCAATGGGGTGCCGGGTTCTTTTTATGGCAGGCTTTTCCCCAACAAAAGTTTGCATTTTGTTCATTCTTCTTACAGTCTTCAATGGCTCTCTCAGGTTCCAAAAGGGATGGAGATAAAAAACAAAGGGAACATATTCATAGACAGTACAAGTCCAGAGAATGTGATTGAAGGGTATTATAGGCAATTTCAAAAGGATTTTTCACTGTTTTTGAAGTGTCGTGGAGAAGAAGTAGTAACTGGTGGACGCATGGGGATCGTTAAAGAGGAGAAAGTTGATAGATTCAATTTTCCAAACTTCATGCCAAGTCCCGAGGAAGTAAAAGCAGAGGTTTTAAAAGAAGGAAGCTTCATCATAAATCGTCTCGAAGTCTCAAGAATTGATTGGAATTTCTACAATACTGAATTTGATGGATCAAATGTGTTTGTTGATAGCAGTTATCATTTTGTAAAATACATGAGATCTGTGCTTCAACCACTTTTGATTTCTCATTTTGGAGAAGTCATTGTAGAAGAATTATTTAAGAGGTACAGACAAATTGTTAAGGATGAG

Protein sequence

MEVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHNKSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVVTGGRMGIVKEEKVDRFNFPNFMPSPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHFGEVIVEELFKRYRQIVKDE
Homology
BLAST of Lsi01G006590 vs. ExPASy Swiss-Prot
Match: Q9SPV4 (Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 4.8e-77
Identity = 150/319 (47.02%), Postives = 201/319 (63.01%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIK---QFHKNL-H 61
           +VIS+  PI + A+    S   + T L IADLGCSSGPN L  V+ LIK   +  K +  
Sbjct: 27  QVISITKPITEAAITALYSGDTVTTRLAIADLGCSSGPNALFAVTELIKTVEELRKKMGR 86

Query: 62  NKSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNK 121
             S +YQIF NDLP NDFNAIFRSLP     ++N +    G CF NGVPGSFYGRLFP  
Sbjct: 87  ENSPEYQIFLNDLPGNDFNAIFRSLP-----IENDVD---GVCFINGVPGSFYGRLFPRN 146

Query: 122 SLHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGE 181
           +LHF+HSSYSL WLSQVP G+E  NKGNI++ +T P++V+  YY+QFQ+D +LFL+CR +
Sbjct: 147 TLHFIHSSYSLMWLSQVPIGIE-SNKGNIYMANTCPQSVLNAYYKQFQEDHALFLRCRAQ 206

Query: 182 EVVTGGRM----------------------------------GIVKEEKVDRFNFPNFMP 241
           EVV GGRM                                  G+++EEK+D+FN P + P
Sbjct: 207 EVVPGGRMVLTILGRRSEDRASTECCLIWQLLAMALNQMVSEGLIEEEKMDKFNIPQYTP 266

Query: 242 SPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLI 283
           SP EV+AE+LKEGSF+I+ +E S I W+    + DG     +  Y+  + MR+V +PLL+
Sbjct: 267 SPTEVEAEILKEGSFLIDHIEASEIYWSSCTKDGDGGGSVEEEGYNVARCMRAVAEPLLL 326

BLAST of Lsi01G006590 vs. ExPASy Swiss-Prot
Match: Q84UB5 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1 OS=Petunia hybrida OX=4102 GN=BSMT1 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 153/320 (47.81%), Postives = 198/320 (61.88%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHN--- 61
           +VI +  PI ++A+ D  S+   P TL IADLGCS G NT ++VS L+K   K       
Sbjct: 27  KVILMTKPITEQAMIDLYSSL-FPETLCIADLGCSLGANTFLVVSQLVKIVEKERKKHGF 86

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           KS ++    NDLP NDFN +F+SL  F EDL+  IG  FG CFF+GVPGSFY RLFP+KS
Sbjct: 87  KSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFPSKS 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFV+SSYSL WLSQVP G+E  NKGNI++  TSP +VI+ YY+Q++ DFS FLK R EE
Sbjct: 147 LHFVYSSYSLMWLSQVPNGIE-NNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEE 206

Query: 182 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 241
           ++ GG+M                                  G++KEEKVD FN P + PS
Sbjct: 207 LMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEGLIKEEKVDAFNIPQYTPS 266

Query: 242 PEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLIS 285
           P EVK  V KEGSF INRLE SR+ WN  N E +G        Y+  + MR+V +PLL+S
Sbjct: 267 PAEVKYIVEKEGSFTINRLETSRVHWNASNNEKNG-------GYNVSRCMRAVAEPLLVS 326

BLAST of Lsi01G006590 vs. ExPASy Swiss-Prot
Match: Q84UB4 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2 OS=Petunia hybrida OX=4102 GN=BSMT2 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-73
Identity = 152/320 (47.50%), Postives = 198/320 (61.88%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHN--- 61
           +VI +  PI ++A+ D  S+   P TL IADLGCS G NT ++VS L+K   K       
Sbjct: 27  KVILMTKPITEQAMIDLYSSL-FPETLCIADLGCSLGANTFLVVSQLVKIVEKERKKHGF 86

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           KS ++    NDLP NDFN +F+SL  F EDL+  IG  FG CFF+GVPGSFY RLFP+KS
Sbjct: 87  KSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFPSKS 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFV+SSYSL WLSQVP G+E  NKGNI++  TSP +VI+ YY+Q++ DFS FLK R EE
Sbjct: 147 LHFVYSSYSLMWLSQVPNGIE-NNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEE 206

Query: 182 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 241
           ++ GG+M                                  G++KEEKVD FN P + PS
Sbjct: 207 LMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEGLIKEEKVDAFNIPQYTPS 266

Query: 242 PEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLIS 285
           P EVK  V KEGSF INRLE SR+ WN  N E +G        Y+  + MR+V +PLL+S
Sbjct: 267 PAEVKYIVEKEGSFTINRLETSRVHWNASNNEKNG-------GYNVSRCMRAVAEPLLVS 326

BLAST of Lsi01G006590 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.4e-73
Identity = 150/320 (46.88%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHN--- 61
           +VI +  PI ++A+ D  S+   P TL IADLGCS G NT ++VS ++K   K       
Sbjct: 27  KVILMTKPITEQAMIDLYSSL-FPETLCIADLGCSLGANTFLVVSQIVKIVEKERKKHGF 86

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           KS ++    NDLP NDFN +F+SL  F EDL+  IG  FG CFF+GVPGSFY RLFP+KS
Sbjct: 87  KSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFPSKS 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFV+SSYSL WLSQVP G+E  NKGNI++  TSP +VI+ YY+Q++ DFS FLK R EE
Sbjct: 147 LHFVYSSYSLMWLSQVPNGIE-NNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRSEE 206

Query: 182 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 241
           ++ GG+M                                  G++KEEKVD FN P + PS
Sbjct: 207 LMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNELVKEGLIKEEKVDAFNIPQYTPS 266

Query: 242 PEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLIS 285
           P EVK  V KEGSF INRLE SR+ WN        SN   +  Y+  + MR+V +PLL+S
Sbjct: 267 PAEVKYLVEKEGSFTINRLETSRVHWN-------ASNNVKNGGYNVSRCMRAVAEPLLVS 326

BLAST of Lsi01G006590 vs. ExPASy Swiss-Prot
Match: A0A061FDP1 (Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 GN=TCM_034089 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.1e-64
Identity = 141/336 (41.96%), Postives = 194/336 (57.74%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHNKSF 61
           ++IS+  PII+EA+ +  S  N+  ++ IADLGCSSGPNTL ++S ++       H    
Sbjct: 27  KIISIGKPIIEEAVHEI-SCNNVLESMGIADLGCSSGPNTLCVISEIMDMVQATSHRLGH 86

Query: 62  ---QYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
              +++++ NDL +NDFN+IF SLP F   LK + G   GSCF +GV GSFYGRLFP+KS
Sbjct: 87  PVPEFRLYLNDLYSNDFNSIFMSLPAFYHRLKEEKGIGCGSCFISGVAGSFYGRLFPSKS 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIK-----NKGNIFIDSTSPENVIEGYYRQFQKDFSLFLK 181
           LH+VHSS SL WLSQVP G+E       NKG ++I  +SP +V+  Y  QFQ DF +F++
Sbjct: 147 LHYVHSSSSLHWLSQVPPGLESNAVTPLNKGKVYISKSSPHSVLHAYSLQFQNDFPMFIE 206

Query: 182 CRGEEVVTGGRM----------------------------------GIVKEEKVDRFNFP 241
            R +E+V+GGRM                                  G+++EEKVD FN P
Sbjct: 207 SRSQELVSGGRMVLSLFGRRSTDPTTEESCYQWELLAQAIMSLVREGLIEEEKVDSFNTP 266

Query: 242 NFMPSPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYH---------- 283
            + P  EE+K E+ KEGSFII+RLE   IDW       DG  V   S  H          
Sbjct: 267 FYAPCAEEIKVEIQKEGSFIIDRLEGFEIDW-------DGGAV---SDVHTAQGKLLIGQ 326

BLAST of Lsi01G006590 vs. ExPASy TrEMBL
Match: A0A5A7T044 (Salicylate carboxymethyltransferase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00050 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 8.2e-120
Identity = 218/319 (68.34%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 5   SLAWPIIKEALEDFCSAQNIP-TTLKIADLGCSSGPNTLMIVSNLIKQFHKNL---HNKS 64
           S  WPIIKEA+ED+   +NIP TTL IADLGCS GPNTL I+S+LIKQFHK +   HNK 
Sbjct: 30  SKVWPIIKEAIEDYLCTENIPITTLSIADLGCSFGPNTLSILSDLIKQFHKTILLHHNKP 89

Query: 65  FQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLH 124
            QYQIF NDLP+NDF+++FRSL NFLEDLKNQIG DFG+CFFNGVPGSFYGRLFPNKSLH
Sbjct: 90  IQYQIFFNDLPSNDFDSLFRSLSNFLEDLKNQIGTDFGTCFFNGVPGSFYGRLFPNKSLH 149

Query: 125 FVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVV 184
           FVHSSY+L WLSQVP+GME+ NKGNIFID TSP+NVIEGYY+QFQKDFSLFLKCRGEE+V
Sbjct: 150 FVHSSYALHWLSQVPEGMEMINKGNIFIDRTSPKNVIEGYYKQFQKDFSLFLKCRGEEIV 209

Query: 185 TGGRM----------------------------------GIVKEEKVDRFNFPNFMPSPE 244
           +GGRM                                  GI++E KVDRFN P FMPSP+
Sbjct: 210 SGGRMVVTLIGRTNESPPKEDFCYTFTLLNLAIYNMVKEGIIREGKVDRFNIPTFMPSPK 269

Query: 245 EVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHF 286
           EVK EVLKEGSFIINR+EV+ IDWN YN EFD SNV VDSSY + K +RSV++PL+I+HF
Sbjct: 270 EVKTEVLKEGSFIINRVEVAGIDWNAYNDEFDESNVLVDSSYCYAKCIRSVIEPLMITHF 329

BLAST of Lsi01G006590 vs. ExPASy TrEMBL
Match: A0A1S4DT98 (salicylate carboxymethyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990440 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 8.2e-120
Identity = 218/319 (68.34%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 5   SLAWPIIKEALEDFCSAQNIP-TTLKIADLGCSSGPNTLMIVSNLIKQFHKNL---HNKS 64
           S  WPIIKEA+ED+   +NIP TTL IADLGCS GPNTL I+S+LIKQFHK +   HNK 
Sbjct: 30  SKVWPIIKEAIEDYLCTENIPITTLSIADLGCSFGPNTLSILSDLIKQFHKTILLHHNKP 89

Query: 65  FQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLH 124
            QYQIF NDLP+NDF+++FRSL NFLEDLKNQIG DFG+CFFNGVPGSFYGRLFPNKSLH
Sbjct: 90  IQYQIFFNDLPSNDFDSLFRSLSNFLEDLKNQIGTDFGTCFFNGVPGSFYGRLFPNKSLH 149

Query: 125 FVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVV 184
           FVHSSY+L WLSQVP+GME+ NKGNIFID TSP+NVIEGYY+QFQKDFSLFLKCRGEE+V
Sbjct: 150 FVHSSYALHWLSQVPEGMEMINKGNIFIDRTSPKNVIEGYYKQFQKDFSLFLKCRGEEIV 209

Query: 185 TGGRM----------------------------------GIVKEEKVDRFNFPNFMPSPE 244
           +GGRM                                  GI++E KVDRFN P FMPSP+
Sbjct: 210 SGGRMVVTLIGRTNESPPKEDFCYTFTLLNLAIYNMVKEGIIREGKVDRFNIPTFMPSPK 269

Query: 245 EVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHF 286
           EVK EVLKEGSFIINR+EV+ IDWN YN EFD SNV VDSSY + K +RSV++PL+I+HF
Sbjct: 270 EVKTEVLKEGSFIINRVEVAGIDWNAYNDEFDESNVLVDSSYCYAKCIRSVIEPLMITHF 329

BLAST of Lsi01G006590 vs. ExPASy TrEMBL
Match: A0A1S3B1W6 (salicylate carboxymethyltransferase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485071 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.2e-118
Identity = 217/322 (67.39%), Postives = 251/322 (77.95%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIP-TTLKIADLGCSSGPNTLMIVSNLIKQFHKNL---H 61
           + +S AWPIIKEA+ED+   +N P TTL IADLGCSSGPNTL I+S+LIKQFHK +   H
Sbjct: 27  KAMSKAWPIIKEAIEDYLCTENTPITTLSIADLGCSSGPNTLSILSDLIKQFHKIIQLHH 86

Query: 62  NKSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNK 121
           NK  QYQIF NDLP+NDFN +FRSL  FLEDLKNQIG DFG+CFFNGVPGSFYGRLFPNK
Sbjct: 87  NKPIQYQIFFNDLPSNDFNFLFRSLSKFLEDLKNQIGTDFGTCFFNGVPGSFYGRLFPNK 146

Query: 122 SLHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGE 181
           SLHFVHSSY+L WLS+VP+GME+ NKGNIFI+ TSP+NVIEGYY+QFQKDFSLFLKCRGE
Sbjct: 147 SLHFVHSSYTLHWLSKVPEGMEMVNKGNIFINRTSPKNVIEGYYKQFQKDFSLFLKCRGE 206

Query: 182 EVVTGGRM----------------------------------GIVKEEKVDRFNFPNFMP 241
           E+V+GGRM                                  G+++EEKVDRFN PNFMP
Sbjct: 207 EIVSGGRMVVTILGRTDESPPNDDFCYTLTLLNLAINNMVKEGMIREEKVDRFNVPNFMP 266

Query: 242 SPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLI 286
           S EEVK EVLKEGSFI+NR+EV+RIDWN YN EF+ SNVFVDSSY + K +RSV +PL+I
Sbjct: 267 SSEEVKTEVLKEGSFIVNRVEVARIDWNSYNDEFNQSNVFVDSSYCYAKCIRSVFEPLMI 326

BLAST of Lsi01G006590 vs. ExPASy TrEMBL
Match: A0A5D3CL38 (Salicylate carboxymethyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001880 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.5e-116
Identity = 215/319 (67.40%), Postives = 246/319 (77.12%), Query Frame = 0

Query: 5   SLAWPIIKEALEDFCSAQNIP-TTLKIADLGCSSGPNTLMIVSNLIKQFHKNL---HNKS 64
           S  WPIIKEA+ED+   +NIP TTL IADLGCS GPNTL I+S+LIKQFHK +   HNK 
Sbjct: 30  SKVWPIIKEAIEDYLCTENIPITTLSIADLGCSFGPNTLSILSDLIKQFHKTILLHHNKP 89

Query: 65  FQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLH 124
            QYQIF NDLP+NDF+++FRSL NFLEDLKNQIG DFG+CFFNGVPGSFYGRLFPNKSLH
Sbjct: 90  IQYQIFFNDLPSNDFDSLFRSLSNFLEDLKNQIGTDFGTCFFNGVPGSFYGRLFPNKSLH 149

Query: 125 FVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVV 184
           FVHSSY+L WLSQVP+GME+ NKGNIFID TSP+NVIEGYY+QFQKDFSLFLKCRGEE+V
Sbjct: 150 FVHSSYALHWLSQVPEGMEMINKGNIFIDRTSPKNVIEGYYKQFQKDFSLFLKCRGEEIV 209

Query: 185 TGGRM----------------------------------GIVKEEKVDRFNFPNFMPSPE 244
           +GGRM                                  GI++E KVDRFN P FMPSP+
Sbjct: 210 SGGRMVVTLIGRTNESPPKEDFCYTFTLLNLAIYNMVKEGIIREGKVDRFNIPTFMPSPK 269

Query: 245 EVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHF 286
           EVK EVLKEGSFIINR+EV+ IDWN YN EFD SNV VDSSY + K +RSV++PL+I+HF
Sbjct: 270 EVKTEVLKEGSFIINRVEVAGIDWNAYNDEFDESNVLVDSSYCYAKCIRSVIEPLMITHF 329

BLAST of Lsi01G006590 vs. ExPASy TrEMBL
Match: A0A6J1ILA6 (salicylate carboxymethyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111478472 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 7.9e-115
Identity = 210/316 (66.46%), Postives = 248/316 (78.48%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQ---FHKNLHN 61
           + IS AWPIIKEAL+DFC+  NIPTT  IADLGC+SGPNTLMIVSNLIKQ   F++NL N
Sbjct: 24  KAISTAWPIIKEALQDFCT-HNIPTTFAIADLGCASGPNTLMIVSNLIKQLHHFYRNLPN 83

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           +S +YQIF NDLPANDFNAIFRSLP  L++LK QIG +FG CFFNGVPGSFYGRLFP+K+
Sbjct: 84  QSLRYQIFFNDLPANDFNAIFRSLPGLLDNLKAQIGGEFGPCFFNGVPGSFYGRLFPDKT 143

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFVHSSYSL WLSQ P+GME++NKGNIFIDSTSPENVIE ++RQFQKDFSLFLKCRGEE
Sbjct: 144 LHFVHSSYSLHWLSQAPEGMEMENKGNIFIDSTSPENVIEAFHRQFQKDFSLFLKCRGEE 203

Query: 182 VVTGGRM-----------------------------GIVKEEKVDRFNFPNFMPSPEEVK 241
           VV GG M                             G+V++EK+ RFN PNFMP+P+E+K
Sbjct: 204 VVAGGGMALTLLGRTDKNYCYAYHLLNLALNKMVAEGMVEKEKMVRFNIPNFMPTPQEIK 263

Query: 242 AEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHFGEV 286
            E+ KEGSF+I RLEVSRIDWNFYN +    +V VDSSY+  K +RSV++PL+I HFGE 
Sbjct: 264 EEIQKEGSFMIKRLEVSRIDWNFYNIDSSSVDVGVDSSYNIAKCIRSVMEPLMIPHFGEP 323

BLAST of Lsi01G006590 vs. NCBI nr
Match: XP_038877755.1 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 458.8 bits (1179), Expect = 3.5e-125
Identity = 231/322 (71.74%), Postives = 249/322 (77.33%), Query Frame = 0

Query: 1   MEVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIK---QFHKNLH 60
           ++VI L WPIIKEALEDF S QNIPTT  IADLGCSSGPNTLMI+SNLIK   Q HK+LH
Sbjct: 24  LQVICLVWPIIKEALEDFWSTQNIPTTFTIADLGCSSGPNTLMIISNLIKQVQQLHKDLH 83

Query: 61  NKSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNK 120
           NK F YQIF NDLP+NDFN IFRSLPNFLEDLK QIGADFG CFFNGVPGSFYGRLFPNK
Sbjct: 84  NKPFDYQIFLNDLPSNDFNTIFRSLPNFLEDLKTQIGADFGRCFFNGVPGSFYGRLFPNK 143

Query: 121 SLHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGE 180
           SLHFVHSSY+L WLSQVP+GMEI NKGNIFIDSTSP+NV+E +YRQFQKDFSLFLKCRGE
Sbjct: 144 SLHFVHSSYTLHWLSQVPEGMEIINKGNIFIDSTSPKNVVEAFYRQFQKDFSLFLKCRGE 203

Query: 181 EVVTGGRM----------------------------------GIVKEEKVDRFNFPNFMP 240
           EVV GGRM                                   I+ EEK +RFN P F P
Sbjct: 204 EVVIGGRMVLTFLGRTDEYPSNKDYCYCLKFLNLALNDMVRERIITEEKANRFNAPTFRP 263

Query: 241 SPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLI 286
            PEEV+AEVLKEGSFIIN  +VSRIDWNFYN EFD SNVFVD SY+F K +RSV +PLLI
Sbjct: 264 CPEEVEAEVLKEGSFIINEFQVSRIDWNFYNIEFDASNVFVDGSYNFAKGIRSVYEPLLI 323

BLAST of Lsi01G006590 vs. NCBI nr
Match: XP_038877754.1 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 457.6 bits (1176), Expect = 7.8e-125
Identity = 231/321 (71.96%), Postives = 248/321 (77.26%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIK---QFHKNLHN 61
           +VI L WPIIKEALEDF S QNIPTT  IADLGCSSGPNTLMI+SNLIK   Q HK+LHN
Sbjct: 27  KVICLVWPIIKEALEDFWSTQNIPTTFTIADLGCSSGPNTLMIISNLIKQVQQLHKDLHN 86

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           K F YQIF NDLP+NDFN IFRSLPNFLEDLK QIGADFG CFFNGVPGSFYGRLFPNKS
Sbjct: 87  KPFDYQIFLNDLPSNDFNTIFRSLPNFLEDLKTQIGADFGRCFFNGVPGSFYGRLFPNKS 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFVHSSY+L WLSQVP+GMEI NKGNIFIDSTSP+NV+E +YRQFQKDFSLFLKCRGEE
Sbjct: 147 LHFVHSSYTLHWLSQVPEGMEIINKGNIFIDSTSPKNVVEAFYRQFQKDFSLFLKCRGEE 206

Query: 182 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 241
           VV GGRM                                   I+ EEK +RFN P F P 
Sbjct: 207 VVIGGRMVLTFLGRTDEYPSNKDYCYCLKFLNLALNDMVRERIITEEKANRFNAPTFRPC 266

Query: 242 PEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLIS 286
           PEEV+AEVLKEGSFIIN  +VSRIDWNFYN EFD SNVFVD SY+F K +RSV +PLLI 
Sbjct: 267 PEEVEAEVLKEGSFIINEFQVSRIDWNFYNIEFDASNVFVDGSYNFAKGIRSVYEPLLIL 326

BLAST of Lsi01G006590 vs. NCBI nr
Match: XP_038877753.1 (salicylate carboxymethyltransferase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 454.1 bits (1167), Expect = 8.7e-124
Identity = 229/322 (71.12%), Postives = 253/322 (78.57%), Query Frame = 0

Query: 1   MEVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQ---FHKNLH 60
           ++ IS+AWPIIKEA+ED CS  NIPTTL IADLGCSSGPNTLMIVS LIKQ   FHK+ H
Sbjct: 24  LQAISIAWPIIKEAIEDLCSTLNIPTTLTIADLGCSSGPNTLMIVSKLIKQIELFHKD-H 83

Query: 61  NKSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNK 120
           NK FQYQIF NDLP NDFN IFRSL NFL DLK +IGADFG CFFNGVPGSFYGRLFP  
Sbjct: 84  NKPFQYQIFLNDLPTNDFNVIFRSLSNFLGDLKTEIGADFGDCFFNGVPGSFYGRLFPKN 143

Query: 121 SLHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGE 180
           +LHFVHSSYSLQWLSQVPK MEI NKGNIFIDSTSP++VIEGYYRQFQKDFSLFLKCRGE
Sbjct: 144 TLHFVHSSYSLQWLSQVPKRMEIMNKGNIFIDSTSPKDVIEGYYRQFQKDFSLFLKCRGE 203

Query: 181 EVVTGGRM----------------------------------GIVKEEKVDRFNFPNFMP 240
           EV+ GGRM                                  GIV+EEKV RFN PNFMP
Sbjct: 204 EVIAGGRMVLTILGRRDEYPSNNDYCHAFTLLNMALNNMVDEGIVEEEKVHRFNIPNFMP 263

Query: 241 SPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLI 286
            PEEV+AEVL+EGSFIIN L VSRIDW+FYNTEFDGSNV+VDSSY+  K +RSV + L+I
Sbjct: 264 CPEEVRAEVLEEGSFIINELGVSRIDWDFYNTEFDGSNVYVDSSYNIAKCIRSVTESLMI 323

BLAST of Lsi01G006590 vs. NCBI nr
Match: XP_038877752.1 (salicylate carboxymethyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 453.0 bits (1164), Expect = 1.9e-123
Identity = 229/321 (71.34%), Postives = 252/321 (78.50%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQ---FHKNLHN 61
           + IS+AWPIIKEA+ED CS  NIPTTL IADLGCSSGPNTLMIVS LIKQ   FHK+ HN
Sbjct: 27  KAISIAWPIIKEAIEDLCSTLNIPTTLTIADLGCSSGPNTLMIVSKLIKQIELFHKD-HN 86

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
           K FQYQIF NDLP NDFN IFRSL NFL DLK +IGADFG CFFNGVPGSFYGRLFP  +
Sbjct: 87  KPFQYQIFLNDLPTNDFNVIFRSLSNFLGDLKTEIGADFGDCFFNGVPGSFYGRLFPKNT 146

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LHFVHSSYSLQWLSQVPK MEI NKGNIFIDSTSP++VIEGYYRQFQKDFSLFLKCRGEE
Sbjct: 147 LHFVHSSYSLQWLSQVPKRMEIMNKGNIFIDSTSPKDVIEGYYRQFQKDFSLFLKCRGEE 206

Query: 182 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 241
           V+ GGRM                                  GIV+EEKV RFN PNFMP 
Sbjct: 207 VIAGGRMVLTILGRRDEYPSNNDYCHAFTLLNMALNNMVDEGIVEEEKVHRFNIPNFMPC 266

Query: 242 PEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLIS 286
           PEEV+AEVL+EGSFIIN L VSRIDW+FYNTEFDGSNV+VDSSY+  K +RSV + L+I 
Sbjct: 267 PEEVRAEVLEEGSFIINELGVSRIDWDFYNTEFDGSNVYVDSSYNIAKCIRSVTESLMIP 326

BLAST of Lsi01G006590 vs. NCBI nr
Match: KAA0036208.1 (salicylate carboxymethyltransferase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 439.9 bits (1130), Expect = 1.7e-119
Identity = 218/319 (68.34%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 5   SLAWPIIKEALEDFCSAQNIP-TTLKIADLGCSSGPNTLMIVSNLIKQFHKNL---HNKS 64
           S  WPIIKEA+ED+   +NIP TTL IADLGCS GPNTL I+S+LIKQFHK +   HNK 
Sbjct: 30  SKVWPIIKEAIEDYLCTENIPITTLSIADLGCSFGPNTLSILSDLIKQFHKTILLHHNKP 89

Query: 65  FQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLH 124
            QYQIF NDLP+NDF+++FRSL NFLEDLKNQIG DFG+CFFNGVPGSFYGRLFPNKSLH
Sbjct: 90  IQYQIFFNDLPSNDFDSLFRSLSNFLEDLKNQIGTDFGTCFFNGVPGSFYGRLFPNKSLH 149

Query: 125 FVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVV 184
           FVHSSY+L WLSQVP+GME+ NKGNIFID TSP+NVIEGYY+QFQKDFSLFLKCRGEE+V
Sbjct: 150 FVHSSYALHWLSQVPEGMEMINKGNIFIDRTSPKNVIEGYYKQFQKDFSLFLKCRGEEIV 209

Query: 185 TGGRM----------------------------------GIVKEEKVDRFNFPNFMPSPE 244
           +GGRM                                  GI++E KVDRFN P FMPSP+
Sbjct: 210 SGGRMVVTLIGRTNESPPKEDFCYTFTLLNLAIYNMVKEGIIREGKVDRFNIPTFMPSPK 269

Query: 245 EVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHF 286
           EVK EVLKEGSFIINR+EV+ IDWN YN EFD SNV VDSSY + K +RSV++PL+I+HF
Sbjct: 270 EVKTEVLKEGSFIINRVEVAGIDWNAYNDEFDESNVLVDSSYCYAKCIRSVIEPLMITHF 329

BLAST of Lsi01G006590 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 221.9 bits (564), Expect = 6.7e-58
Identity = 134/340 (39.41%), Postives = 188/340 (55.29%), Query Frame = 0

Query: 3   VISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHK---NLHNK 62
           +ISL   ++ EAL+    + +  +++ IADLGCSSGPN+L+ +SN++   H    +L   
Sbjct: 28  IISLGRRVMDEALKKLMMSNSEISSIGIADLGCSSGPNSLLSISNIVDTIHNLCPDLDRP 87

Query: 63  SFQYQIFSNDLPANDFNAIFRSLPNFLEDL-KNQIGADFG-----SCFFNGVPGSFYGRL 122
             + ++  NDLP+NDFN I  SLP F + +  N+ G  FG     SCF + VPGSFYGRL
Sbjct: 88  VPELRVSLNDLPSNDFNYICASLPEFYDRVNNNKEGLGFGRGGGESCFVSAVPGSFYGRL 147

Query: 123 FPNKSLHFVHSSYSLQWLSQVP----------KGMEIKNKGNIFIDSTSPENVIEGYYRQ 182
           FP +SLHFVHSS SL WLSQVP             +++N G I+I  TSP++  + Y  Q
Sbjct: 148 FPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTITADLENMGKIYISKTSPKSAHKAYALQ 207

Query: 183 FQKDFSLFLKCRGEEVVTGGRM----------------------------------GIVK 242
           FQ DF +FL+ R EE+V GGRM                                  GI++
Sbjct: 208 FQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDPTTEESCYQWELLAQALMSMAKEGIIE 267

Query: 243 EEKVDRFNFPNFMPSPEEVKAEVLKEGSFIINRLEVSRIDW-------NFYNTEFDGSNV 283
           EEK+D FN P +  S EE+K  + KEGSF I+RLE+S IDW         Y+        
Sbjct: 268 EEKIDAFNAPYYAASSEELKMVIEKEGSFSIDRLEISPIDWEGGSISEESYDLVIRSKPE 327

BLAST of Lsi01G006590 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-54
Identity = 117/315 (37.14%), Postives = 177/315 (56.19%), Query Frame = 0

Query: 2   EVISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFH---KNLHN 61
           +V+S+A P++    E+     + PT +K+A+LGCSSG N+ + +  +I   +   ++++ 
Sbjct: 49  KVLSMAKPVLVRNTEEMMMNLDFPTYIKVAELGCSSGQNSFLAIFEIINTINVLCQHVNK 108

Query: 62  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 121
            S +     NDLP NDFN  F+ +P F ++L   +  +  SCF  G PGSFY RLF   S
Sbjct: 109 NSPEIDCCLNDLPENDFNTTFKFVPFFNKEL---MITNKSSCFVYGAPGSFYSRLFSRNS 168

Query: 122 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 181
           LH +HSSY+L WLS+VP+ +E  NKGN++I S+SP++  + Y  QFQKDF++FL+ R EE
Sbjct: 169 LHLIHSSYALHWLSKVPEKLE-NNKGNLYITSSSPQSAYKAYLNQFQKDFTMFLRLRSEE 228

Query: 182 VVTGGRM-----------------------------------GIVKEEKVDRFNFPNFMP 241
           +V+ GRM                                   G+V E K+D FN P + P
Sbjct: 229 IVSNGRMVLTFIGRNTLNDPLYRDCCHFWTLLSNSLRDLVFEGLVSESKLDAFNMPFYDP 288

Query: 242 SPEEVKAEVLKEGSFIINRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLI 279
           + +E+K  + KEGSF IN LE    D   Y  E D      ++  +    +R+V +P+LI
Sbjct: 289 NVQELKEVIQKEGSFEINELESHGFDLGHYYEEDD-----FEAGRNEANGIRAVSEPMLI 348

BLAST of Lsi01G006590 vs. TAIR 10
Match: AT3G21950.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.3e-53
Identity = 119/323 (36.84%), Postives = 173/323 (53.56%), Query Frame = 0

Query: 3   VISLAWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLM----IVSNLIKQFHKNLHN 62
           + S A P + +++ +     + P  +K+ADLGCSSG NT +    IV+ +I  + +N  N
Sbjct: 25  ITSDAKPEVMKSVNEMIVKMDFPGCIKVADLGCSSGENTFLVMSEIVNTIITTYQQNGQN 84

Query: 63  KSFQYQIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKS 122
              +     NDLP NDFN  F+ +P+F E LK  +    G+C+ +G PGSFY RLFP+KS
Sbjct: 85  LP-EIDCCLNDLPENDFNTTFKLIPSFHEKLKMNVK---GNCYVSGCPGSFYTRLFPSKS 144

Query: 123 LHFVHSSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEE 182
           LHFVHSS+ L WLS+VP G+E +NK N+++ S  P N+ E Y+ QF+KDFS+FL+ R EE
Sbjct: 145 LHFVHSSFCLHWLSKVPDGLE-ENKKNVYLRSPCPPNLYESYWNQFKKDFSMFLRMRAEE 204

Query: 183 VVTGGRM----------------------------------GIVKEEKVDRFNFPNFMPS 242
            +  GRM                                  G+VKE  ++ FN P + P 
Sbjct: 205 TMPSGRMALTLVGRKTLDPLSKECFKDWSLVSDSLLDLVSEGVVKESDLESFNLPYYSPD 264

Query: 243 PEEVKAEVLKEGSFIINRLEV---SRIDWNFYNTEFDGSNVFVDSSYHF------VKYMR 279
             EVK  +  EGSF I   E        +   ++E    +  VD S  F          R
Sbjct: 265 ESEVKEVIENEGSFEIKNFETIFGLLFSYKTGHSEVKDDDDDVDHSRRFEVVKTRANMTR 324

BLAST of Lsi01G006590 vs. TAIR 10
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 120/318 (37.74%), Postives = 166/318 (52.20%), Query Frame = 0

Query: 7   AWPIIKEALEDFCSAQNIPTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHNKSF---QY 66
           A P++ E +++     + P  +K+ADLGCSSG NTL+++S ++     +   K     + 
Sbjct: 29  AKPVVVETVKEMIVKTDFPGCIKVADLGCSSGENTLLVMSEIVNTIITSYQQKGKNLPEI 88

Query: 67  QIFSNDLPANDFNAIFRSLPNFLEDLKNQIGADFGSCFFNGVPGSFYGRLFPNKSLHFVH 126
               NDLP NDFN  F+ +P F + LK  +    G CF +GVPGSFY RLFP+KSLHFVH
Sbjct: 89  NCCLNDLPDNDFNTTFKLVPAFHKLLKMDVK---GKCFISGVPGSFYSRLFPSKSLHFVH 148

Query: 127 SSYSLQWLSQVPKGMEIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVVTGG 186
           SS  L WLS+VP G+E  NK N+++ S  P NV + Y  QF+ DFSLFL+ R +E V  G
Sbjct: 149 SSLCLHWLSKVPDGLE-DNKKNVYLRSPCPPNVYKSYLTQFKNDFSLFLRLRADETVPNG 208

Query: 187 RM----------------------------------GIVKEEKVDRFNFPNFMPSPEEVK 246
           RM                                  GIVKE  VD FN P + P   EV+
Sbjct: 209 RMALTFVGRKSLDPLSKDCFQNWSSISDSLLDLVSEGIVKESDVDSFNLPFYNPDESEVR 268

Query: 247 AEVLKEGSFIINRLEV---SRIDWNFYNTEFDGSNVFVDSSYHF------VKYMRSVLQP 279
             +  EGSF I+  E        +    TE    +  +D S  F         +RS+ +P
Sbjct: 269 EVIESEGSFKISNFETIFGLLFSYKTGRTEVKDDDDNLDQSCRFEVIRKRASIIRSITEP 328

BLAST of Lsi01G006590 vs. TAIR 10
Match: AT4G36470.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 3.6e-51
Identity = 120/306 (39.22%), Postives = 171/306 (55.88%), Query Frame = 0

Query: 25  PTTLKIADLGCSSGPNTLMIVSNLIKQFHKNLHNK-----SFQYQIFSNDLPANDFNAIF 84
           P +L IADLGCSSGPNTL  +++ IK      H +       ++ IF NDLP NDFN IF
Sbjct: 52  PKSLGIADLGCSSGPNTLSTITDFIKTVQVAHHREIPIQPLPEFSIFLNDLPGNDFNFIF 111

Query: 85  RSLPNFLEDLK-NQIGADFGSCFFNGVPGSFYGRLFPNKSLHFVHSSYSLQWLSQVPKGM 144
           +SLP+F  +LK +    D  S F    PGSFYGRLFP  ++HFV++S+SL WLS+VP  +
Sbjct: 112 KSLPDFHIELKRDNNNGDCPSVFIAAYPGSFYGRLFPENTIHFVYASHSLHWLSKVPTAL 171

Query: 145 -----EIKNKGNIFIDSTSPENVIEGYYRQFQKDFSLFLKCRGEEVVTGGRM-------- 204
                +  NKG + I S S E V + Y  QF++DFS+FL+CR +E+V+ GRM        
Sbjct: 172 YDEQGKSINKGCVSICSLSSEAVSKAYCSQFKEDFSIFLRCRSKEMVSAGRMVLIILGRE 231

Query: 205 --------------------------GIVKEEKVDRFNFPNFMPSPEEVKAEVLKEGSFI 264
                                     G  +EEK+D ++   + PS +E++ EV KEGSF 
Sbjct: 232 GPDHVDRGNSFFWELLSRSIADLVAQGETEEEKLDSYDMHFYAPSADEIEGEVDKEGSFE 291

Query: 265 INRLEVSRIDWNFYNTEFDGSNVFVDSSYHFVKYMRSVLQPLLISHFGEVIVEELFKRYR 286
           + RLE+  +  +  NTE D     +       K +R+V + +L+ HFGE I+++LF  Y 
Sbjct: 292 LERLEMLEVKKDKGNTEGD-----ISYGKAVAKTVRAVQESMLVQHFGEKILDKLFDTYC 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPV44.8e-7747.02Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=... [more]
Q84UB51.1e-7347.81S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1... [more]
Q84UB41.5e-7347.50S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2... [more]
A4ZDG89.4e-7346.88S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
A0A061FDP16.1e-6441.96Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 G... [more]
Match NameE-valueIdentityDescription
A0A5A7T0448.2e-12068.34Salicylate carboxymethyltransferase-like isoform X1 OS=Cucumis melo var. makuwa ... [more]
A0A1S4DT988.2e-12068.34salicylate carboxymethyltransferase-like OS=Cucumis melo OX=3656 GN=LOC107990440... [more]
A0A1S3B1W61.2e-11867.39salicylate carboxymethyltransferase-like isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A5D3CL382.5e-11667.40Salicylate carboxymethyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1ILA67.9e-11566.46salicylate carboxymethyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
Match NameE-valueIdentityDescription
XP_038877755.13.5e-12571.74S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
XP_038877754.17.8e-12571.96S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
XP_038877753.18.7e-12471.12salicylate carboxymethyltransferase-like isoform X2 [Benincasa hispida][more]
XP_038877752.11.9e-12371.34salicylate carboxymethyltransferase-like isoform X1 [Benincasa hispida][more]
KAA0036208.11.7e-11968.34salicylate carboxymethyltransferase-like isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G19640.16.7e-5839.41jasmonic acid carboxyl methyltransferase [more]
AT3G11480.11.2e-5437.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G21950.11.3e-5336.84S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G38020.11.1e-5237.74S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G36470.13.6e-5139.22S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 4..236
e-value: 5.1E-86
score: 289.6
NoneNo IPR availablePANTHERPTHR31009:SF49OS05G0102000 PROTEINcoord: 186..283
NoneNo IPR availablePANTHERPTHR31009:SF49OS05G0102000 PROTEINcoord: 3..185
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 14..185
e-value: 1.1E-69
score: 235.3
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 3..185
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 186..283
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 2..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G006590.1Lsi01G006590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity