Lsi01G006540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G006540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	abscisic acid 8'-hydroxylase 1-like
Location	chr01: 5257789 .. 5261269 (+)
RNA-Seq Expression	Lsi01G006540
Synteny	Lsi01G006540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATATATTGCAGCATCTTGGAAGAGTATTTCCCTTCCCCCATCACATTGGAACCATTCATTGTTTTCATCAATAGTCAATGGAGTTTGTGTGTGAAAAGTTGAAGATGTTGTGTAAGGAAGGGCTTGTGTTCTTAGCTCAAAACTACTTTAACACCACACTGGCAGCACTTCTTTCCATAGCTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGTAGCAAGAATGTTGGAGGAATTCCTGGTCAGTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGACTCCGCAGGTTGTGGTAAGTGGTTCTCTCCCTCATGGTGATTATGGATATTCTTTACACAAGTACTATTGTCAGTATCACTTCTAATATTCCTTTAATATGTTTCCAGTTTTCCTCATTCAAGGCCATCAAGTTTGGCCCATTTTTTCTAAAATCTTTGATTTATATATATAAGATGATTCCTTAAGCTTCTATAATTTGATAATTTTTGAACTTTTGGCTACAAAACTAGACTTTGCCTATCATCGCATATGAGTCAACTCCAATGATTTTTTACACTTAAGACTGCTGATGTGGGTTGCATGGCTATTTGGGAAGGTAGATATACACACATACATACACACATATATACATAGATAGATTAGTATATACATCCCAGGCAACTTAGAACATGGAATTGATGACTGTGGTGACACTAAACCAGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATACATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGAGATGCTGTTGGAGAGAGTAGTTTACTATGCGTTTCCCATGAGAATCACAGAAGGATTAGACGTCTTCTAGCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTTGACGCCATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTTAGCATGAAGGTTACAGAAAAATAAAAACTCAAGTCATCTTTCTCAAACTTGTCTGTTTGACTTGGGCAGTCAACATATCTTATTCTTATGTTTGTGATTTGCAGATAACATTTGATTCCATGTGTAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAGAAAGACTGTACAGCCGTCTCTGAGGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGTACAGTCAGAAGCTTCAAGATATAGGCACCCCAATTTACCTGTATACAATTTCATGAGGAGTTTTTGGCTTCATTCAACTTTAGAAACATATATACTAATTACCAACATGTTGAGCAGGCACGAAGCCGACTAATGGAAAGATTTAGAGAGATAATTTCCCAACGGCGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTTCGAATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAGGCCAAACCACCACAGCTGCTGCCATGATGTGGAGTATCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGTAAGATACACAACCTGTTAAGTTACTTTGAAAAAAGCTAACAAACGTTAACCATAATCCACGGCTCTTCATTCTTTTCATTCCAAGCGCTCCTGTGAAGTGAATACTAATAAGTAACACCCATTTCAAATATCCAGGAAGAACAATTGTCAATTCTACAAAAGAAGGAAGATGGAGAATTGCTTACCCTTCAAGATCTTAACAAAATGTCCTACGGTTCAAAGGTTATAAGTAGCCTAAGTTTGGTCTTATTTTGTAAGAAGTATACAGAGTGCTTCAGATTGTAAAGAATTCCATTCACAATGTACGAAGTGGCCATTAACAACCTAATTCATGTACAATTGCAGGTTGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGCACGTTTTTCCTGTTACTGTCATTCTCTCTGCTTTTGATATTTAACTAAATGGAAGGAAATTTACAAGATTTTCAACCGTAGGAAAGGAAGATGATTCCTGTAGAACTATTTCTGCTGCTAGAATAATAACATATACGTATAAAGAAAGGCATTCTATTCATAGGCAAATAGATAGAGAAATATGCCATGTGGACTTGATATGAAGTTTTTGAACAAATTGATTCAACAGAGGGAACCAGGAACCCCATCCAACTAATTTTGCACGTGATATGCAGGTTTTGAAATAAAGGAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGTACAAAGACCCCATGCAATTCAACCCTTCTAGATTTGATGTATCTACCTAAAATCAATCACTGTTTCTTCTTGTTCTTTTTTCCTTCTTTGGCTACTAAACATATATTATTGTTCATGCACCTATGGGGCATAACTCCAGGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGGCCCAGGACCTGTCTTGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCACAGAATGACTGGAGGGTACAAGTGAGTTTGTACATTCCTTAAGCAGCAGGCATTTCATCATGAGTTCCAAGATATAATTCAGAATTTTCCTCTTCTTCTTCTTCTTCTTCTTATCCTTATCATAATTCTGTTGCCTTTGTATTGTTTAGGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACACATCCCTAGGCTGAGAAGCGGATGCCCCATTACCTTGAAGACCTTGTAAGATGGGACAAAAGCATAGATGTGAGAACTTTATCTCAGGTATGGATACAAGTGGCAAGATTCTAATGCAAGGAAATGAGATAGGTTAGATGTCTTCAAATGTGAGGAAGCAGTTGGATAAAATATGTATCTTCATGGCCTCTAATTACTTGCTCTTTTCTAAGCTTCACATAAAAGCAAATCAGAAGAGAAAAAGACAAACAGGAACTTTGCAACAATGCAGTATGAACGAATAACTATTACTGGAGTCTATATAAAATTCAATATAATTTGAGAAAACTAAGTTAAGCAAGAGTTCCTGCCTGTCATGCATTTCATAAGATCCGGTTACCTGTTTTGTTCGACTTTGCACACCTATGAAGCCCCTTCACTCTAGATTCTCCATCTGTCTGACTCCGTGGTTTACTTTCTACTTACCATTGAAGTATATTTCTTTTTAAAGATTCTAATCTCTCTGGTTAATATGTGTGTAATGCTTGATAATGTTAAATTTTCAATATTTTATGATATACATGTCATTTCAAACCCACCCC

mRNA sequence

TATATATTGCAGCATCTTGGAAGAGTATTTCCCTTCCCCCATCACATTGGAACCATTCATTGTTTTCATCAATAGTCAATGGAGTTTGTGTGTGAAAAGTTGAAGATGTTGTGTAAGGAAGGGCTTGTGTTCTTAGCTCAAAACTACTTTAACACCACACTGGCAGCACTTCTTTCCATAGCTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGTAGCAAGAATGTTGGAGGAATTCCTGGTCAGTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGACTCCGCAGGTTGTGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATACATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGAGATGCTGTTGGAGAGAGTAGTTTACTATGCGTTTCCCATGAGAATCACAGAAGGATTAGACGTCTTCTAGCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTTGACGCCATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTTAGCATGAAGATAACATTTGATTCCATGTGTAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAGAAAGACTGTACAGCCGTCTCTGAGGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAGCCGACTAATGGAAAGATTTAGAGAGATAATTTCCCAACGGCGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTTCGAATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAGGCCAAACCACCACAGCTGCTGCCATGATGTGGAGTATCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGAAGAACAATTGTCAATTCTACAAAAGAAGGAAGATGGAGAATTGCTTACCCTTCAAGATCTTAACAAAATGTCCTACGGTTCAAAGGTTGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGCACGACCTGTCTTGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCACAGAATGACTGGAGGGTACAAGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACACATCCCTAGGCTGAGAAGCGGATGCCCCATTACCTTGAAGACCTTGTAAGATGGGACAAAAGCATAGATGTGAGAACTTTATCTCAGGTATGGATACAAGTGGCAAGATTCTAATGCAAGGAAATGAGATAGGTTAGATGTCTTCAAATGTGAGGAAGCAGTTGGATAAAATATGTATCTTCATGGCCTCTAATTACTTGCTCTTTTCTAAGCTTCACATAAAAGCAAATCAGAAGAGAAAAAGACAAACAGGAACTTTGCAACAATGCAGTATGAACGAATAACTATTACTGGAGTCTATATAAAATTCAATATAATTTGAGAAAACTAAGTTAAGCAAGAGTTCCTGCCTGTCATGCATTTCATAAGATCCGGTTACCTGTTTTGTTCGACTTTGCACACCTATGAAGCCCCTTCACTCTAGATTCTCCATCTGTCTGACTCCGTGGTTTACTTTCTACTTACCATTGAAGTATATTTCTTTTTAAAGATTCTAATCTCTCTGGTTAATATGTGTGTAATGCTTGATAATGTTAAATTTTCAATATTTTATGATATACATGTCATTTCAAACCCACCCC

Coding sequence (CDS)

ATGGAGTTTGTGTGTGAAAAGTTGAAGATGTTGTGTAAGGAAGGGCTTGTGTTCTTAGCTCAAAACTACTTTAACACCACACTGGCAGCACTTCTTTCCATAGCTATTTTAGTGTTGGTTTCGAAAGTTTGCTGGAGACAGAGTAGCAAGAATGTTGGAGGAATTCCTGGTCAGTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCTAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGACTCCGCAGGTTGTGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATACATGTGTTTGTTCCAAGTGCAGAAGGTGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGATACGTGAAATCCATGGGAGATGCTGTTGGAGAGAGTAGTTTACTATGCGTTTCCCATGAGAATCACAGAAGGATTAGACGTCTTCTAGCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTTGACGCCATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTGCTTGATTTTAGCATGAAGATAACATTTGATTCCATGTGTAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGATTGAGAAAGACTGTACAGCCGTCTCTGAGGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAGCCGACTAATGGAAAGATTTAGAGAGATAATTTCCCAACGGCGGAGGGGAGAGGAGTCACCAGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTTCGAATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTGACACTGATAATTGCAGGCCAAACCACCACAGCTGCTGCCATGATGTGGAGTATCAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGGCTGCGGGAAGAACAATTGTCAATTCTACAAAAGAAGGAAGATGGAGAATTGCTTACCCTTCAAGATCTTAACAAAATGTCCTACGGTTCAAAGGTTGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGTGTGGCACTAGGGGATTGCAGATTAGAAGGCACGACCTGTCTTGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCACAGAATGACTGGAGGGTACAAGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACACATCCCTAGGCTGAGAAGCGGATGCCCCATTACCTTGAAGACCTTGTAA

Protein sequence

MEFVCEKLKMLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEGTTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPITLKTL

Homology

[Close]

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 4.6e-34
Identity = 111/412 (26.94%), Postives = 183/412 (44.42%), Query Frame = 0


Query: 56  PGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKA 115
           PG +G P+VGETF   S + +      F + ++  +G  FKT + G   V + S E AK 
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKF 97

Query: 116 IFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAM 175
           +       F   +  S    +G+ ++     + H ++R+L+   F   S+   V + +++
Sbjct: 98  VLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESI 157

Query: 176 LSKRLKKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSE-AMLSF 235
               L+    +G            TF+     +   +DE   R+  K C  + E    S 
Sbjct: 158 AQDSLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSM 217

Query: 236 PFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSE 295
           P  +PGT ++K +KAR  L +    I+S+RR+   S  D L S +        E+L D +
Sbjct: 218 PVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQ 277

Query: 296 IMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLN 355
           I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + KE+GE LT  D  
Sbjct: 278 IADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTK 337

Query: 356 KMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEG-------------------------- 415
           KM   S+V++ETLR++++L +  R A+ D   EG                          
Sbjct: 338 KMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNP 397

Query: 416 ------------------------TTCLGINMAKLTMLVFLHRMTGGYKWTV 417
                                    +C G  +AKL M + +H +T  Y W++
Sbjct: 398 GKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSI 436

[Close]

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.0e-34
Identity = 121/469 (25.80%), Postives = 205/469 (43.71%), Query Frame = 0


Query: 24  FNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFSFLSANNSTKGCYHF 83
           F T  AA L + +L  ++ V  R SS  +   PG +G P+VGETF   S + +      F
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV-----F 66

Query: 84  VRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVGESSLLC 143
              ++  +G  FKT + G   V + S E AK +       F   +  S    +G+ ++  
Sbjct: 67  FAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 126

Query: 144 VSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFSMKITFDS 203
              + H ++R+L+   F   ++   V   +++  + L     DG            TF+ 
Sbjct: 127 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNV 186

Query: 204 MCNMLMSLRDESTLRQIEKDCTAVSE-AMLSFPFMIPGTRYYKGIKARSRLMERFREIIS 263
               ++  +DE   R+  K C  + E    S P  +PGT ++K +KAR  L +    I+S
Sbjct: 187 ALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILS 246

Query: 264 QRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLD 323
           +RR+   S  D L S ++         L D +I DN++ +I A + TTA+ + W +K+L 
Sbjct: 247 KRRQNPSSHTDLLGSFME-----DKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLA 306

Query: 324 ENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNVLLWFPRVALG 383
           +N    E + EEQ++I + K++GE LT +D  KM    +V++ETLR + +L +  R A+ 
Sbjct: 307 DNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 366

Query: 384 DCRLEG--------------------------------------------------TTCL 442
           D   EG                                                   +C 
Sbjct: 367 DVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCP 426

[Close]

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-32
Identity = 130/481 (27.03%), Postives = 203/481 (42.20%), Query Frame = 0


Query: 24  FNTTLAALLSIA-ILVLVSK---VCWRQSSKNVGGIPGQLGLPFVGETFSFLSANNSTKG 83
           F   LA+ + +A    L+S      WR      G  PG  G P +GET  FL        
Sbjct: 23  FTVFLASFIGLAFFFYLISNHRTKAWR------GIPPGTFGWPLIGETLEFLGCQRKGNP 82

Query: 84  CYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVG-- 143
              F   R   +G  F T L G   V   S EG + +F+N+    NK  V S   +VG  
Sbjct: 83  -RDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNE----NKLVVNSWPSSVGNL 142

Query: 144 -ESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFS 203
             SSL+    ++ +R+RR+L       +L +FV   D+M  + L +        + L   
Sbjct: 143 FRSSLITTVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLL 202

Query: 204 MKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKARSRLMER 263
            + TF   C++  S+  +  L ++        + ++  P  +PGTRY K   A + + ++
Sbjct: 203 KRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQ 262

Query: 264 FREIISQRR----RGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAA 323
              II++R+     G  SPE  L S L  +     E L D+EI DN+L L+ AG  T+++
Sbjct: 263 LGSIINERKIALEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSS 322

Query: 324 AMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNV 383
            +   +KFL EN    E +  EQL+I   KE+G+LL  +DL +M Y  +V +E LR+   
Sbjct: 323 TLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPA 382

Query: 384 LLWFPRVALGDCRLEGTT------------------------------------------ 441
           +    R A+ +   +G T                                          
Sbjct: 383 VQGSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTF 442

[Close]

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.6e-32
Identity = 105/413 (25.42%), Postives = 176/413 (42.62%), Query Frame = 0


Query: 56  PGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKA 115
           PG +G P++GET    S + +      F   ++  +G  FKT + G   V + S E A+ 
Sbjct: 46  PGSMGWPYIGETLQLYSQDPNV-----FFASKQKRYGEIFKTHILGCPCVMLASPEAARF 105

Query: 116 IFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAM 175
           +       F   Y +S    +G S+L     + H R+R+L+  P    +L   V + +A 
Sbjct: 106 VLVTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 165

Query: 176 LSKRLKKLVKDGKSFVLLDFSMKITFD-SMCNMLMSLRDESTLRQIEKDCTAVSEAMLSF 235
           +   L     DG          +++FD  +  +     DE    ++ ++   V +   SF
Sbjct: 166 VRSTLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSF 225

Query: 236 PFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSE 295
           P   PGT YYK I+AR RL     +I+ +RR   E   D L  ++Q  +      L D +
Sbjct: 226 PNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQ 285

Query: 296 IMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGEL-LTLQDL 355
           + DN++ ++ A Q TTA+ + W +K+L ++ +  E +R EQ +I    + G L LT    
Sbjct: 286 VADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQT 345

Query: 356 NKMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEG------------------------- 415
             M+   KV+ E+LRM++++ +  R A+ D   +G                         
Sbjct: 346 RSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQD 405

Query: 416 -------------------------TTCLGINMAKLTMLVFLHRMTGGYKWTV 417
                                      C G  +AKL MLV +H +  GY+W +
Sbjct: 406 PQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEI 451

[Close]

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.6e-32
Identity = 105/413 (25.42%), Postives = 176/413 (42.62%), Query Frame = 0


Query: 56  PGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKA 115
           PG +G P++GET    S + +      F   ++  +G  FKT + G   V + S E A+ 
Sbjct: 46  PGSMGWPYIGETLQLYSQDPNV-----FFASKQKRYGEIFKTHILGCPCVMLASPEAARF 105

Query: 116 IFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAM 175
           +       F   Y +S    +G S+L     + H R+R+L+  P    +L   V + +A 
Sbjct: 106 VLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 165

Query: 176 LSKRLKKLVKDGKSFVLLDFSMKITFD-SMCNMLMSLRDESTLRQIEKDCTAVSEAMLSF 235
           +   L     DG          +++FD  +  +     DE    ++ ++   V +   SF
Sbjct: 166 VRSTLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSF 225

Query: 236 PFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSE 295
           P   PGT YYK I+AR RL     +I+ +RR   E   D L  ++Q  +      L D +
Sbjct: 226 PNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQ 285

Query: 296 IMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGEL-LTLQDL 355
           + DN++ ++ A Q TTA+ + W +K+L ++ +  E +R EQ +I    + G L LT    
Sbjct: 286 VADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQT 345

Query: 356 NKMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEG------------------------- 415
             M+   KV+ E+LRM++++ +  R A+ D   +G                         
Sbjct: 346 RSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQD 405

Query: 416 -------------------------TTCLGINMAKLTMLVFLHRMTGGYKWTV 417
                                      C G  +AKL MLV +H +  GY+W +
Sbjct: 406 PQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEI 451

[Close]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL
Match: A0A0A0KKH5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504430 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.0e-223
Identity = 405/484 (83.68%), Postives = 417/484 (86.16%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           MLCKEGLVF+AQ YFN T+AALLSIA+LVLVSKV WR  SKN GGIPG LGLPFVGETFS
Sbjct: 1   MLCKEGLVFIAQMYFNPTVAALLSIAVLVLVSKVWWRVRSKNDGGIPGYLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF+NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFSNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDS+CNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSICNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R RLME FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RKRLMETFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMW IKFLDEN+EAQERLREEQLSILQKKEDGELLTL+DL KMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWCIKFLDENKEAQERLREEQLSILQKKEDGELLTLEDLKKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMKFNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDP LEKKAHIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPSLEKKAHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL
Match: A0A5A7SHU1 (Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001790 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.5e-221
Identity = 402/484 (83.06%), Postives = 415/484 (85.74%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           M+C EGL F+AQ YFN T+AALLSIAILVLVSKV WR  SKN G IPG LGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQIEKDC AVS+AMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R+RLME+FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELL L+DLNKMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEFNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL
Match: A0A1S3B1V7 (abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo OX=3656 GN=LOC103485076 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.5e-221
Identity = 402/484 (83.06%), Postives = 415/484 (85.74%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           M+C EGL F+AQ YFN T+AALLSIAILVLVSKV WR  SKN G IPG LGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQIEKDC AVS+AMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R+RLME+FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELL L+DLNKMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEYNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL
Match: A0A6J1IMR3 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.3e-220
Identity = 399/485 (82.27%), Postives = 419/485 (86.39%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFN-TTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETF 69
           ML KE LV LAQNYF+ TT+AA+LS+ ILVLVSKVCWRQ SKNVGGIPG LGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 70  SFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGY 129
           SFLSANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 130 VKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGK 189
           VKSMGDAVGE SLLCVSHE+HRRIRRLLADPFSMTSLSKFVK+FD MLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 190 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIK 249
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAV++AMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 250 ARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQT 309
           AR+RLMER  EIIS+RRRGEESPEDFLQSMLQRDSYP ++KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 310 TTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLR 369
           TTAAAMMWS+KFLDENREAQERLREEQLSIL+KKEDGELLTL+DLNKMS+GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 370 MSNVLLWFPRVALGDCRLEG---------------------------------------- 429
           MSNVLLWFPRVALGDCRLEG                                        
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 430 ----------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPI 444
                      TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

[Close]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL
Match: A0A6J1GG59 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.4e-219
Identity = 399/485 (82.27%), Postives = 419/485 (86.39%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFN-TTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETF 69
           ML KE LV LAQNYF+ TT+AA+LSI ILVLVSKVCWRQ SKNVGGIPG LGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 70  SFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGY 129
           SFLSANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 130 VKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGK 189
           VKSMGDAVGE SLLCVSHE+HRRIRRLLADPFSMTSLSKFVK+FD MLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 190 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIK 249
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDC+AVS+AMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 250 ARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQT 309
           AR+RLMER REIIS+RR+GEESPEDFLQSMLQRDSYP ++KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 310 TTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLR 369
           TTAAAMMWS+KFLDENREAQERLREEQLSIL+KKEDGELLTL+DLNKMS+GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 370 MSNVLLWFPRVALGDCRLEG---------------------------------------- 429
           MSNVLLWFPRVALGDCRLEG                                        
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 430 ----------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPI 444
                      TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

[Close]

BLAST of Lsi01G006540 vs. NCBI nr
Match: XP_038883385.1 (abscisic acid 8'-hydroxylase 3-like [Benincasa hispida] >XP_038883386.1 abscisic acid 8'-hydroxylase 3-like [Benincasa hispida])

HSP 1 Score: 794.7 bits (2051), Expect = 4.2e-226
Identity = 410/484 (84.71%), Postives = 421/484 (86.98%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           MLCKEGLVFLAQNYF+ T+AALLSIAIL LVSKVCWRQ SKNVGGIPG LGLPFVGETFS
Sbjct: 1   MLCKEGLVFLAQNYFDPTVAALLSIAILALVSKVCWRQRSKNVGGIPGNLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLLADPFSMTSLS FVKEFD MLSKRLKKLVKDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLADPFSMTSLSNFVKEFDDMLSKRLKKLVKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDSMC+MLMSLRDESTLRQIEKDC AVS+AMLSFP MIP TRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCHMLMSLRDESTLRQIEKDCIAVSDAMLSFPVMIPETRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R+RLM+RFREII +RRRGEESP+DFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMKRFREIIFRRRRGEESPKDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMWSIKFLDENREAQERLREEQLSIL+ KEDGELLTL+DLNKMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRNKEDGELLTLEDLNKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLEG                                         
Sbjct: 361 SNVLLWFPRVALGDCRLEGFEIKEGWHVNIDATCIHYDPDLYKDPMQFTPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. NCBI nr
Match: XP_004150006.2 (abscisic acid 8'-hydroxylase 3 [Cucumis sativus] >KGN48862.1 hypothetical protein Csa_004271 [Cucumis sativus])

HSP 1 Score: 782.7 bits (2020), Expect = 1.7e-222
Identity = 405/484 (83.68%), Postives = 417/484 (86.16%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           MLCKEGLVF+AQ YFN T+AALLSIA+LVLVSKV WR  SKN GGIPG LGLPFVGETFS
Sbjct: 1   MLCKEGLVFIAQMYFNPTVAALLSIAVLVLVSKVWWRVRSKNDGGIPGYLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF+NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFSNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDS+CNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSICNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R RLME FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RKRLMETFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMW IKFLDEN+EAQERLREEQLSILQKKEDGELLTL+DL KMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWCIKFLDENKEAQERLREEQLSILQKKEDGELLTLEDLKKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMKFNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDP LEKKAHIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPSLEKKAHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. NCBI nr
Match: KAA0025720.1 (abscisic acid 8'-hydroxylase 1-like [Cucumis melo var. makuwa] >TYK12595.1 abscisic acid 8'-hydroxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 778.5 bits (2009), Expect = 3.1e-221
Identity = 402/484 (83.06%), Postives = 415/484 (85.74%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           M+C EGL F+AQ YFN T+AALLSIAILVLVSKV WR  SKN G IPG LGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQIEKDC AVS+AMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R+RLME+FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELL L+DLNKMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEFNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. NCBI nr
Match: XP_008440758.1 (PREDICTED: abscisic acid 8'-hydroxylase 1-like [Cucumis melo])

HSP 1 Score: 778.5 bits (2009), Expect = 3.1e-221
Identity = 402/484 (83.06%), Postives = 415/484 (85.74%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFS 69
           M+C EGL F+AQ YFN T+AALLSIAILVLVSKV WR  SKN G IPG LGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 70  FLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 129
           FLSANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF NDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 130 KSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKS 189
           KSMGDAVGE SLLCVSHENHRRIRRLL+DPFSMTSLSKFVKEFD MLSKRLKKL KDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 190 FVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKA 249
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQIEKDC AVS+AMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 250 RSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 309
           R+RLME+FREIIS+RRRGEESPEDFL+SMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 310 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRM 369
           TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELL L+DLNKMSYGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 370 SNVLLWFPRVALGDCRLEG----------------------------------------- 429
           SNVLLWFPRVALGDCRLE                                          
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEYNPSRFDEIQKPY 420

Query: 430 ---------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPIT 444
                     TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

[Close]

BLAST of Lsi01G006540 vs. NCBI nr
Match: XP_022978466.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 399/485 (82.27%), Postives = 419/485 (86.39%), Query Frame = 0


Query: 10  MLCKEGLVFLAQNYFN-TTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETF 69
           ML KE LV LAQNYF+ TT+AA+LS+ ILVLVSKVCWRQ SKNVGGIPG LGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 70  SFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGY 129
           SFLSANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 130 VKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGK 189
           VKSMGDAVGE SLLCVSHE+HRRIRRLLADPFSMTSLSKFVK+FD MLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 190 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIK 249
           SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAV++AMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 250 ARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQT 309
           AR+RLMER  EIIS+RRRGEESPEDFLQSMLQRDSYP ++KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 310 TTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLR 369
           TTAAAMMWS+KFLDENREAQERLREEQLSIL+KKEDGELLTL+DLNKMS+GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 370 MSNVLLWFPRVALGDCRLEG---------------------------------------- 429
           MSNVLLWFPRVALGDCRLEG                                        
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 430 ----------TTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKAHIPRLRSGCPI 444
                      TCLGINMAKLTMLVFLHRMTGGY+WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

[Close]

BLAST of Lsi01G006540 vs. TAIR 10
Match: AT1G19630.1 (cytochrome P450, family 722, subfamily A, polypeptide 1 )

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-83
Identity = 186/468 (39.74%), Postives = 261/468 (55.77%), Query Frame = 0


Query: 28  LAALLSIAILVL--VSKVCWRQSSKNVGGI-PGQLGLPFVGETFSFLSANNSTKGCYHFV 87
           L  +L  A+L L    K+ ++   K+  G+ PG  G P +GET  F+ + NS KG Y FV
Sbjct: 6   LCLVLCAAMLTLGKFLKIMFQDRKKSTAGVPPGSDGFPVIGETLQFMLSVNSGKGFYEFV 65

Query: 88  RLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVGESSLLCV 147
           R RR+ +G  F+T LFG+ HVF+ + E A+ +  ND   F K Y+KS+G+ VG+ SLLC 
Sbjct: 66  RSRRIRYGSCFRTSLFGETHVFLSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCA 125

Query: 148 SHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFSMKITFDSM 207
              +H+ +R  L + FS  S +  V+ FD ++   L      G + VLL   ++ITF +M
Sbjct: 126 PQHHHKILRSRLINLFSKRSTALMVRHFDELVVDALGGWEHRG-TVVLLTDLLQITFKAM 185

Query: 208 CNMLMSLRDESTLRQIEKDCTAVSEAMLSFPFMIPGTRYYKGIKARSRLMERFREIISQR 267
           C ML+SL  E  L  +++D   V EAML+FP  +P TR++KGI AR R+ME   +II +R
Sbjct: 186 CKMLVSLEKEEELGSMQRDVGFVCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRER 245

Query: 268 RRGEES----PEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKF 327
           R    S     EDFLQ +L  D+     +L D+EI DN+LT+IIAGQ TTA+A+ W +K+
Sbjct: 246 RNEINSHNNHHEDFLQQLLAVDN--DTPQLTDAEIKDNILTMIIAGQDTTASALTWMVKY 305

Query: 328 LDENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNVLLWFPRVA 387
           L EN++  + L EEQ  I +K  +   L L+DL++M Y SK+VKE+LRM++V+ WFPR+ 
Sbjct: 306 LGENQKVLDILIEEQSQITKKASNKPFLELEDLSEMPYASKMVKESLRMASVVPWFPRLV 365

Query: 388 LGDCRLE--------------------------------------------------GTT 439
           L DC +E                                                  G T
Sbjct: 366 LQDCEMEGYKIKKGWNINIDARSIHLDPTVYSEPHKFNPLRFEEEAKANSFLAFGMGGRT 425

[Close]

BLAST of Lsi01G006540 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 147.1 bits (370), Expect = 3.3e-35
Identity = 111/412 (26.94%), Postives = 183/412 (44.42%), Query Frame = 0


Query: 56  PGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKA 115
           PG +G P+VGETF   S + +      F + ++  +G  FKT + G   V + S E AK 
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKF 97

Query: 116 IFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAM 175
           +       F   +  S    +G+ ++     + H ++R+L+   F   S+   V + +++
Sbjct: 98  VLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESI 157

Query: 176 LSKRLKKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSE-AMLSF 235
               L+    +G            TF+     +   +DE   R+  K C  + E    S 
Sbjct: 158 AQDSLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSM 217

Query: 236 PFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSE 295
           P  +PGT ++K +KAR  L +    I+S+RR+   S  D L S +        E+L D +
Sbjct: 218 PVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQ 277

Query: 296 IMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLN 355
           I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + KE+GE LT  D  
Sbjct: 278 IADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTK 337

Query: 356 KMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEG-------------------------- 415
           KM   S+V++ETLR++++L +  R A+ D   EG                          
Sbjct: 338 KMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNP 397

Query: 416 ------------------------TTCLGINMAKLTMLVFLHRMTGGYKWTV 417
                                    +C G  +AKL M + +H +T  Y W++
Sbjct: 398 GKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSI 436

[Close]

BLAST of Lsi01G006540 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 146.7 bits (369), Expect = 4.3e-35
Identity = 121/469 (25.80%), Postives = 205/469 (43.71%), Query Frame = 0


Query: 24  FNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFSFLSANNSTKGCYHF 83
           F T  AA L + +L  ++ V  R SS  +   PG +G P+VGETF   S + +      F
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV-----F 66

Query: 84  VRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVGESSLLC 143
              ++  +G  FKT + G   V + S E AK +       F   +  S    +G+ ++  
Sbjct: 67  FAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 126

Query: 144 VSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFSMKITFDS 203
              + H ++R+L+   F   ++   V   +++  + L     DG            TF+ 
Sbjct: 127 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNV 186

Query: 204 MCNMLMSLRDESTLRQIEKDCTAVSE-AMLSFPFMIPGTRYYKGIKARSRLMERFREIIS 263
               ++  +DE   R+  K C  + E    S P  +PGT ++K +KAR  L +    I+S
Sbjct: 187 ALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILS 246

Query: 264 QRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLD 323
           +RR+   S  D L S ++         L D +I DN++ +I A + TTA+ + W +K+L 
Sbjct: 247 KRRQNPSSHTDLLGSFME-----DKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLA 306

Query: 324 ENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNVLLWFPRVALG 383
           +N    E + EEQ++I + K++GE LT +D  KM    +V++ETLR + +L +  R A+ 
Sbjct: 307 DNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 366

Query: 384 DCRLEG--------------------------------------------------TTCL 442
           D   EG                                                   +C 
Sbjct: 367 DVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCP 426

[Close]

BLAST of Lsi01G006540 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 145.6 bits (366), Expect = 9.5e-35
Identity = 101/334 (30.24%), Postives = 166/334 (49.70%), Query Frame = 0


Query: 56  PGQLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKA 115
           PG +G P+VGETF   S + +      F + ++  +G  FKT + G   V + S E AK 
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKF 97

Query: 116 IFANDFVQFNKGYVKSMGDAVGESSLLCVSHENHRRIRRLLADPFSMTSLSKFVKEFDAM 175
           +       F   +  S    +G+ ++     + H ++R+L+   F   S+   V + +++
Sbjct: 98  VLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESI 157

Query: 176 LSKRLKKLVKDGKSFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSE-AMLSF 235
               L+    +G            TF+     +   +DE   R+  K C  + E    S 
Sbjct: 158 AQDSLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSM 217

Query: 236 PFMIPGTRYYKGIKARSRLMERFREIISQRRRGEESPEDFLQSMLQRDSYPSNEKLDDSE 295
           P  +PGT ++K +KAR  L +    I+S+RR+   S  D L S +        E+L D +
Sbjct: 218 PVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQ 277

Query: 296 IMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLTLQDLN 355
           I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + KE+GE LT  D  
Sbjct: 278 IADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTK 337

Query: 356 KMSYGSKVVKETLRMSNVLLWFPRVALGDCRLEG 389
           KM   S+V++ETLR++++L +  R A+ D   EG
Sbjct: 338 KMPLTSRVIQETLRVASILSFTFREAVEDVEYEG 358

[Close]

BLAST of Lsi01G006540 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 106/366 (28.96%), Postives = 177/366 (48.36%), Query Frame = 0


Query: 24  FNTTLAALLSIAILVLVSKVCWRQSSKNVGGIPGQLGLPFVGETFSFLSANNSTKGCYHF 83
           F T  AA L + +L  ++ V  R SS  +   PG +G P+VGETF   S + +      F
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV-----F 66

Query: 84  VRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYVKSMGDAVGESSLLC 143
              ++  +G  FKT + G   V + S E AK +       F   +  S    +G+ ++  
Sbjct: 67  FAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 126

Query: 144 VSHENHRRIRRLLADPFSMTSLSKFVKEFDAMLSKRLKKLVKDGKSFVLLDFSMKITFDS 203
              + H ++R+L+   F   ++   V   +++  + L     DG            TF+ 
Sbjct: 127 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNV 186

Query: 204 MCNMLMSLRDESTLRQIEKDCTAVSE-AMLSFPFMIPGTRYYKGIKARSRLMERFREIIS 263
               ++  +DE   R+  K C  + E    S P  +PGT ++K +KAR  L +    I+S
Sbjct: 187 ALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILS 246

Query: 264 QRRRGEESPEDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLD 323
           +RR+   S  D L S ++         L D +I DN++ +I A + TTA+ + W +K+L 
Sbjct: 247 KRRQNPSSHTDLLGSFME-----DKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLA 306

Query: 324 ENREAQERLREEQLSILQKKEDGELLTLQDLNKMSYGSKVVKETLRMSNVLLWFPRVALG 383
           +N    E + EEQ++I + K++GE LT +D  KM    +V++ETLR + +L +  R A+ 
Sbjct: 307 DNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 358

Query: 384 DCRLEG 389
           D   EG
Sbjct: 367 DVEYEG 358

The following BLAST results are available for this feature:

BLAST of Lsi01G006540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949P1	4.6e-34	26.94	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ...	[more]
Q9FH76	6.0e-34	25.80	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ...	[more]
Q50EK1	1.5e-32	27.03	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
A2Z212	5.6e-32	25.42	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q0J185	5.6e-32	25.42	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]

BLAST of Lsi01G006540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKH5	8.0e-223	83.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504430 PE=3 SV=1	[more]
A0A5A7SHU1	1.5e-221	83.06	Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3B1V7	1.5e-221	83.06	abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo OX=3656 GN=LOC103485076 PE=3...	[more]
A0A6J1IMR3	8.3e-220	82.27	abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GG59	1.4e-219	82.27	abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Lsi01G006540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883385.1	4.2e-226	84.71	abscisic acid 8'-hydroxylase 3-like [Benincasa hispida] >XP_038883386.1 abscisic...	[more]
XP_004150006.2	1.7e-222	83.68	abscisic acid 8'-hydroxylase 3 [Cucumis sativus] >KGN48862.1 hypothetical protei...	[more]
KAA0025720.1	3.1e-221	83.06	abscisic acid 8'-hydroxylase 1-like [Cucumis melo var. makuwa] >TYK12595.1 absci...	[more]
XP_008440758.1	3.1e-221	83.06	PREDICTED: abscisic acid 8'-hydroxylase 1-like [Cucumis melo]	[more]
XP_022978466.1	1.7e-219	82.27	abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Lsi01G006540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19630.1	1.1e-83	39.74	cytochrome P450, family 722, subfamily A, polypeptide 1	[more]
AT4G19230.1	3.3e-35	26.94	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.1	4.3e-35	25.80	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.2	9.5e-35	30.24	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.2	1.1e-33	28.96	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 324..344
None	No IPR available	PANTHER	PTHR24286:SF235	CYTOCHROME P450 FAMILY PROTEIN	coord: 390..441 coord: 26..388
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 390..441 coord: 26..388
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 391..414 score: 21.66 coord: 294..311 score: 28.47 coord: 314..340 score: 20.49
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 56..389 e-value: 6.2E-37 score: 127.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 45..390 e-value: 1.2E-72 score: 247.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 45..441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G006540.1	Lsi01G006540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen