Lsi01G005440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G005440
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	endochitinase-like
Location	chr01: 4417050 .. 4417910 (+)
RNA-Seq Expression	Lsi01G005440
Synteny	Lsi01G005440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAATTCGACCCTATAAATATCTTCACTTCCCTTCTCATTTTCTTCATATCTTTTCTCATTTGATACACAAACAAGAAAAACAATGAAGCTTCATAGCCTTATAATCTTAGCCTTTGCGTTTCTTTTGGGAGCTGCCTCAGCTGAGCAATGTGGGCGACAAGCCAATGGAGCTCTATGCCCCAATAACCTTTGCTGCAGCCAGTTCGGGTGGTGCGGCGACACCGACGACTATTGTAAAACCGGTTGCCAAAGCCAATGTCGTGGCTCAACCCCTTCTACTCCTCCTAGCTCCGGTGGCGGCAGCATCGGTGGGATTATTAGTGAAGGCTTTTACAATCAAATGCTCAAGTATAGTAGGGACGCTCGATGCCCTAGTAATGGATTCTATACCTATAATGCTTTCATTACTGCCGCTCAATCCTTCCCTGGCTTTGGTACCACGGGAGATGACGCTACTCGTAAGAGGGAGCTCGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGTTTGTTTTAAAACTACCTTTATTCTTTCAATAATTTGCAATTTCATCCTTCACTTGAGAATAAATATAGAGCAATGATTATGTGCAATTCTTTTACACAATTTCTTTTTGTTTTTGCCACATGACAATTTTTTATTGAACAACATCTAAATTTTTCTCATAAATATATATATAAACAAACTCTTGTGATATAGGAGGATGGGCTAGTGCACCCGATGGTCCATATGCATGGGGATACTGTTTCATACGGGAGATAAACCAAGACGTATATTGCACACCTAACCAACAATGGCCATGTGTTGCTGGTCAGAAATATTACGGCCGTGGACCAATTCAACTAACCCAGTAA

mRNA sequence

ATCAAATTCGACCCTATAAATATCTTCACTTCCCTTCTCATTTTCTTCATATCTTTTCTCATTTGATACACAAACAAGAAAAACAATGAAGCTTCATAGCCTTATAATCTTAGCCTTTGCGTTTCTTTTGGGAGCTGCCTCAGCTGAGCAATGTGGGCGACAAGCCAATGGAGCTCTATGCCCCAATAACCTTTGCTGCAGCCAGTTCGGGTGGTGCGGCGACACCGACGACTATTGTAAAACCGGTTGCCAAAGCCAATGTCGTGGCTCAACCCCTTCTACTCCTCCTAGCTCCGGTGGCGGCAGCATCGGTGGGATTATTAGTGAAGGCTTTTACAATCAAATGCTCAAGTATAGTAGGGACGCTCGATGCCCTAGTAATGGATTCTATACCTATAATGCTTTCATTACTGCCGCTCAATCCTTCCCTGGCTTTGGTACCACGGGAGATGACGCTACTCGTAAGAGGGAGCTCGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGAGGATGGGCTAGTGCACCCGATGGTCCATATGCATGGGGATACTGTTTCATACGGGAGATAAACCAAGACGTATATTGCACACCTAACCAACAATGGCCATGTGTTGCTGGTCAGAAATATTACGGCCGTGGACCAATTCAACTAACCCAGTAA

Coding sequence (CDS)

ATGAAGCTTCATAGCCTTATAATCTTAGCCTTTGCGTTTCTTTTGGGAGCTGCCTCAGCTGAGCAATGTGGGCGACAAGCCAATGGAGCTCTATGCCCCAATAACCTTTGCTGCAGCCAGTTCGGGTGGTGCGGCGACACCGACGACTATTGTAAAACCGGTTGCCAAAGCCAATGTCGTGGCTCAACCCCTTCTACTCCTCCTAGCTCCGGTGGCGGCAGCATCGGTGGGATTATTAGTGAAGGCTTTTACAATCAAATGCTCAAGTATAGTAGGGACGCTCGATGCCCTAGTAATGGATTCTATACCTATAATGCTTTCATTACTGCCGCTCAATCCTTCCCTGGCTTTGGTACCACGGGAGATGACGCTACTCGTAAGAGGGAGCTCGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGAGGATGGGCTAGTGCACCCGATGGTCCATATGCATGGGGATACTGTTTCATACGGGAGATAAACCAAGACGTATATTGCACACCTAACCAACAATGGCCATGTGTTGCTGGTCAGAAATATTACGGCCGTGGACCAATTCAACTAACCCAGTAA

Protein sequence

MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCRGSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAGQKYYGRGPIQLTQ

Homology

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BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot
Match: P19171 (Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3)

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-72
Identity = 126/194 (64.95%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCK-TGCQSQC 60
           MK +  + L F+ LL  +SAEQCGRQA GALCPN LCCS+FGWCG+T+ YCK  GCQSQC
Sbjct: 14  MKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQC 73

Query: 61  RGSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGT 120
              TP   P    G + GIIS   ++ MLK+  DA CP+ GFYTYNAFITAA+SFPGFGT
Sbjct: 74  ---TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGT 133

Query: 121 TGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYCTPNQQWPCV 180
           TGD ATRK+E+AAFFGQTSHETTGGWA+APDGPY+WGYCF +E N    YC P+  WPC 
Sbjct: 134 TGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCA 193

Query: 181 AGQKYYGRGPIQLT 193
           +G++YYGRGP+QL+
Sbjct: 194 SGKRYYGRGPMQLS 204

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BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.1e-71
Identity = 126/194 (64.95%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCK-TGCQSQC 60
           MK   L+ L F+FLL  + AEQCGRQA GALCPN LCCS+FGWCGDT+ YCK  GCQSQC
Sbjct: 1   MKSCLLLFLIFSFLLSFSLAEQCGRQAGGALCPNGLCCSEFGWCGDTEAYCKQPGCQSQC 60

Query: 61  RGSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGT 120
            G TP  P     G + GIIS   ++ MLK+  D  CP+ GFYTY+AFI AA+SFPGFGT
Sbjct: 61  -GGTPPGPT----GDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGT 120

Query: 121 TGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYCTPNQQWPCV 180
           TGD ATRK+E+AAFFGQTSHETTGGWA+APDGPY+WGYCF +E N    YC+P+ +WPC 
Sbjct: 121 TGDTATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCA 180

Query: 181 AGQKYYGRGPIQLT 193
           +G+ YYGRGP+QL+
Sbjct: 181 SGKSYYGRGPMQLS 189

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BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 265.8 bits (678), Expect = 3.9e-70
Identity = 123/194 (63.40%), Postives = 148/194 (76.29%), Query Frame = 0


Query: 2   KLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTG-CQSQCR 61
           K  +L  L F+ LL +ASAEQCG QA GA CP+ LCCS+FGWCG+T+DYC  G CQSQC 
Sbjct: 5   KFTALSSLLFSLLLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQCP 64

Query: 62  GS-TPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGT 121
           G  TP+ P   GGG +G IIS   ++QMLK+  D  C   GFY+YNAFI AA+SFPGFGT
Sbjct: 65  GGPTPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGT 124

Query: 122 TGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREI-NQDVYCTPNQQWPCV 181
           +GD   RKRE+AAFF QTSHETTGGWA+APDGPYAWGYC++RE  +   YCTP+ QWPC 
Sbjct: 125 SGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCA 184

Query: 182 AGQKYYGRGPIQLT 193
            G+KY+GRGPIQ++
Sbjct: 185 PGRKYFGRGPIQIS 198

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BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot
Match: P36907 (Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 6.6e-70
Identity = 123/191 (64.40%), Postives = 146/191 (76.44%), Query Frame = 0


Query: 5   SLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCRGS-T 64
           S  IL    L   + AEQCG QA GA+CPN LCCS+FG+CG TD YC  GCQSQC+ S T
Sbjct: 8   SFFILCLLPLFLGSKAEQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQSQCKSSPT 67

Query: 65  PSTP-PSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGD 124
           P+ P PS+GGG +G ++    ++QMLKY  D RC  +GFYTY+AFI AA+SF GFGTTGD
Sbjct: 68  PTIPTPSTGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIAAARSFNGFGTTGD 127

Query: 125 DATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYCTPNQQWPCVAGQ 184
           D T+K+ELAAF  QTSHETTGGW +APDGPYAWGYCF+ E N Q+VYC+P + WPC  G+
Sbjct: 128 DNTKKKELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSP-KDWPCAPGK 187

Query: 185 KYYGRGPIQLT 193
           KYYGRGPIQLT
Sbjct: 188 KYYGRGPIQLT 197

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BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-69
Identity = 121/188 (64.36%), Postives = 137/188 (72.87%), Query Frame = 0


Query: 6   LIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCRGSTPS 65
           L++L    L G A AEQCGRQA GALCP  LCCSQFGWCG T DYC   CQSQC G TPS
Sbjct: 11  LLLLLVGLLAGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGGVTPS 70

Query: 66  TPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGDDAT 125
                 GG +  +IS+  +NQMLK+  DA C + GFYTYNAFI AA SF GF + GD AT
Sbjct: 71  P-----GGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTAT 130

Query: 126 RKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREI-NQDVYCTPNQQWPCVAGQKYY 185
           RKRE+AAF  QTSHETTGGWA+APDGPYAWGYCF++E  N   YC P  QWPC  G+KYY
Sbjct: 131 RKREIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYY 190

Query: 186 GRGPIQLT 193
           GRGPIQ++
Sbjct: 191 GRGPIQIS 193

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BLAST of Lsi01G005440 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.4e-99
Identity = 168/192 (87.50%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKLH+LI+LAFAF+LG A AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 8   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           GSTPSTPPSSGG  +G IISE  YNQMLKYS+D RCPSNGFY YNAF+TAAQSFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIRE NQDVYC+PNQQWPC AG
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 188 QKYYGRGPIQLT 198

[Close]

BLAST of Lsi01G005440 vs. ExPASy TrEMBL
Match: A0A6J1ILX6 (chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.0e-99
Identity = 168/192 (87.50%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKL +LI+LAFAF+LGAA AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 1   MKLRTLIVLAFAFVLGAA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCG 60

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           G+TPSTPPSSGGG +G IISE  YNQMLKYS+D RCPSNGFY YNAFI A QSFPGFGTT
Sbjct: 61  GTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIREINQDVYC+PNQQWPC AG
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 181 QKYYGRGPIQLT 191

[Close]

BLAST of Lsi01G005440 vs. ExPASy TrEMBL
Match: A0A1S3B2Y3 (chitinase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485182 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.6e-97
Identity = 167/192 (86.98%), Postives = 176/192 (91.67%), Query Frame = 0


Query: 2   KLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCRG 61
           +L  LIILAFAFLLG+ SAEQCGRQANGALCPNNLCCSQ+GWCGDTD YCK GCQSQCRG
Sbjct: 4   QLSCLIILAFAFLLGSTSAEQCGRQANGALCPNNLCCSQYGWCGDTDAYCKDGCQSQCRG 63

Query: 62  STPSTPPSSGGGS-IGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 121
           ST  TPPS+  GS +G II+E  Y QMLKYS D+RCPSNGFYTYNAFITAAQSFPGFGTT
Sbjct: 64  STSPTPPSTPSGSGVGSIITESLYKQMLKYSTDSRCPSNGFYTYNAFITAAQSFPGFGTT 123

Query: 122 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 181
           GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTP+ QWPCV+G
Sbjct: 124 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPSSQWPCVSG 183

Query: 182 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 184 QKYYGRGPIQLT 195

[Close]

BLAST of Lsi01G005440 vs. ExPASy TrEMBL
Match: A0A6J1GD34 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.6e-96
Identity = 166/194 (85.57%), Postives = 177/194 (91.24%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKLH+LI+LAFAF+LGAA AEQCGRQANGALCPN LCCSQFGWCG+TD+YCK  CQSQC 
Sbjct: 1   MKLHALIVLAFAFVLGAA-AEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQCG 60

Query: 61  GSTPSTPPSS--GGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFG 120
           GSTPS PPS+  GGGS+G II+E  YNQMLKYS+D RCPSNGFY YNAFITAAQSFP FG
Sbjct: 61  GSTPSPPPSTGGGGGSVGSIINEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFPAFG 120

Query: 121 TTGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCV 180
           TTGD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIRE NQDVYC+PNQQWPC 
Sbjct: 121 TTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCA 180

Query: 181 AGQKYYGRGPIQLT 193
           AGQKYYGRGPIQLT
Sbjct: 181 AGQKYYGRGPIQLT 193

[Close]

BLAST of Lsi01G005440 vs. ExPASy TrEMBL
Match: A0A5A7SHJ5 (Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00730 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.1e-94
Identity = 162/192 (84.38%), Postives = 173/192 (90.10%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MK +SL+IL+FAFLLGAASAEQCGRQANGALCPNNLCCSQFG+CGDTDDYCK GCQSQCR
Sbjct: 1   MKTYSLVILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           GS  STPP SGG  +G IISE  YNQMLKYSRD RCPSNGFYTYNAFITAA+SFP FGTT
Sbjct: 61  GS--STPPPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTT 120

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD  TRKRE+AAFFGQTSHETTGGW++APDGPYAWGYCFIRE NQ  YCTP+QQWPC  G
Sbjct: 121 GDATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCAPG 180

Query: 181 QKYYGRGPIQLT 193
           Q+YYGRGPIQLT
Sbjct: 181 QQYYGRGPIQLT 190

[Close]

BLAST of Lsi01G005440 vs. NCBI nr
Match: KAG6603867.1 (hypothetical protein SDJN03_04476, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 372.9 bits (956), Expect = 1.7e-99
Identity = 169/192 (88.02%), Postives = 178/192 (92.71%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKLH+LI+LAFAF+LG A AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 1   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 60

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           GSTPSTPPSSGG  +G IISE  YNQMLKYS+D RCPSNGFY YNAF+TAAQSFPGFGTT
Sbjct: 61  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 120

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIREINQDVYC+PNQQWPC AG
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 181 QKYYGRGPIQLT 191

[Close]

BLAST of Lsi01G005440 vs. NCBI nr
Match: XP_038883175.1 (endochitinase-like [Benincasa hispida])

HSP 1 Score: 370.9 bits (951), Expect = 6.5e-99
Identity = 179/236 (75.85%), Postives = 185/236 (78.39%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDD----------- 60
           MK HSLIIL+F FLLGAASAEQCGRQANGALCPNNLCCSQ+GWCGDTDD           
Sbjct: 1   MKAHSLIILSFVFLLGAASAEQCGRQANGALCPNNLCCSQYGWCGDTDDYCKTGCQSQCR 60

Query: 61  ---------------------------------YCKTGCQSQCRGSTPSTPPSSGGGSIG 120
                                            YCKTGCQSQCRGSTPSTPPSS GG +G
Sbjct: 61  GSSVEQCGRQANGALCPNNLCCSQYGWCGDTDAYCKTGCQSQCRGSTPSTPPSS-GGDVG 120

Query: 121 GIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGDDATRKRELAAFFGQ 180
           GII+EG YNQMLKYS+DARCPSNGFYTYNAFITAAQSFPGFGTTGDD+TRK ELAAFFGQ
Sbjct: 121 GIINEGLYNQMLKYSKDARCPSNGFYTYNAFITAAQSFPGFGTTGDDSTRKSELAAFFGQ 180

Query: 181 TSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAGQKYYGRGPIQLT 193
           TSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPN QWPCVAGQKYYGRGPIQLT
Sbjct: 181 TSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNNQWPCVAGQKYYGRGPIQLT 235

[Close]

BLAST of Lsi01G005440 vs. NCBI nr
Match: XP_023544980.1 (endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.5 bits (950), Expect = 8.5e-99
Identity = 168/192 (87.50%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKLH+LI+LAFAF+LG A AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 1   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 60

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           GSTPSTPPSSGG  +G IISE  YNQMLKYS+D RCPSNGFY YNAF+TAAQSFPGFGTT
Sbjct: 61  GSTPSTPPSSGGSGVGSIISEALYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 120

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIRE NQDVYC+PNQQWPC AG
Sbjct: 121 GDTATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 180

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 181 QKYYGRGPIQLT 191

[Close]

BLAST of Lsi01G005440 vs. NCBI nr
Match: XP_022949791.1 (endochitinase-like [Cucurbita moschata])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 168/192 (87.50%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKLH+LI+LAFAF+LG A AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 8   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           GSTPSTPPSSGG  +G IISE  YNQMLKYS+D RCPSNGFY YNAF+TAAQSFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIRE NQDVYC+PNQQWPC AG
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 188 QKYYGRGPIQLT 198

[Close]

BLAST of Lsi01G005440 vs. NCBI nr
Match: XP_022977305.1 (chitinase 1-like [Cucurbita maxima])

HSP 1 Score: 369.8 bits (948), Expect = 1.5e-98
Identity = 168/192 (87.50%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCR 60
           MKL +LI+LAFAF+LGAA AEQCGRQANGALCPN LCCSQFGWCGDTD+YCK GCQSQC 
Sbjct: 1   MKLRTLIVLAFAFVLGAA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCG 60

Query: 61  GSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTT 120
           G+TPSTPPSSGGG +G IISE  YNQMLKYS+D RCPSNGFY YNAFI A QSFPGFGTT
Sbjct: 61  GTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120

Query: 121 GDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQWPCVAG 180
           GD ATRKRELAAFFGQTSHETTGGWA+APDGPYAWGYCFIREINQDVYC+PNQQWPC AG
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180

Query: 181 QKYYGRGPIQLT 193
           QKYYGRGPIQLT
Sbjct: 181 QKYYGRGPIQLT 191

[Close]

BLAST of Lsi01G005440 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 274.2 bits (700), Expect = 7.8e-74
Identity = 126/194 (64.95%), Postives = 150/194 (77.32%), Query Frame = 0


Query: 1   MKLHSLIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCK-TGCQSQC 60
           MK +  + L F+ LL  +SAEQCGRQA GALCPN LCCS+FGWCG+T+ YCK  GCQSQC
Sbjct: 14  MKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQC 73

Query: 61  RGSTPSTPPSSGGGSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGT 120
              TP   P    G + GIIS   ++ MLK+  DA CP+ GFYTYNAFITAA+SFPGFGT
Sbjct: 74  ---TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGT 133

Query: 121 TGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYCTPNQQWPCV 180
           TGD ATRK+E+AAFFGQTSHETTGGWA+APDGPY+WGYCF +E N    YC P+  WPC 
Sbjct: 134 TGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCA 193

Query: 181 AGQKYYGRGPIQLT 193
           +G++YYGRGP+QL+
Sbjct: 194 SGKRYYGRGPMQLS 204

[Close]

BLAST of Lsi01G005440 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-34
Identity = 64/120 (53.33%), Postives = 84/120 (70.00%), Query Frame = 0


Query: 75  IGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGDDATRKRELAAFF 134
           I  ++    Y+Q+  +  +  CP+ GFY Y AF+ A +SFP FG+ G+  TR+RE+AAF 
Sbjct: 38  IMSLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFL 97

Query: 135 GQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYC-TPNQQWPCVAGQKYYGRGPIQLT 193
            Q SHETTGGWA+APDGPYAWG CF  E++ Q  YC   N+ WPCV+G+ Y GRGPIQL+
Sbjct: 98  AQISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLS 157

[Close]

BLAST of Lsi01G005440 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-33
Identity = 61/121 (50.41%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 74  SIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSFPGFGTTGDDATRKRELAAF 133
           SI  ++    YN++  +  +  CP+NGFYTY +F+ A + FP FG+ G   T++ E+AAF
Sbjct: 29  SIERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAF 88

Query: 134 FGQTSHETTGGWASAPDGPYAWGYCFIREIN-QDVYC-TPNQQWPCVAGQKYYGRGPIQL 193
             Q SHETTGGWA+APDGPYAWG CF  E++ Q  YC + + QWPC   + Y GRGPIQL
Sbjct: 89  LAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQL 148

[Close]

BLAST of Lsi01G005440 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.1e-30
Identity = 79/199 (39.70%), Postives = 107/199 (53.77%), Query Frame = 0


Query: 5   SLIILAF--AFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQSQCRGS 64
           +LII  F    + G A ++ CG   NG  C  N+CCS++G+CG T  YC TGCQS    S
Sbjct: 11  ALIIFLFTLTIMTGTAFSQNCG--TNG--CKGNMCCSRWGYCGTTKAYCGTGCQSGPCNS 70

Query: 65  TPS---TPPSSGG------GSIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQS 124
            P    TP  SGG      G+I  +I+  F+N ++     + CP+ GFYT  AFI AA+S
Sbjct: 71  KPKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMS-KVGSGCPAKGFYTRQAFIAAAES 130

Query: 125 FPGFGTTGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQ 184
           F  +  T      KRE+AA   Q SHE             +  +C+  EI +  YC+P+ 
Sbjct: 131 FAAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGRYCSPST 187

Query: 185 QWPCVAGQKYYGRGPIQLT 193
            +PC  G+ YYGRGPIQ+T
Sbjct: 191 TYPCQPGKNYYGRGPIQIT 187

[Close]

BLAST of Lsi01G005440 vs. TAIR 10
Match: AT2G43620.1 (Chitinase family protein )

HSP 1 Score: 128.3 bits (321), Expect = 6.9e-30
Identity = 72/198 (36.36%), Postives = 102/198 (51.52%), Query Frame = 0


Query: 6   LIILAFAFLLGAASAEQCGRQANGALCPNNLCCSQFGWCGDTDDYCKTGCQS-QCRGSTP 65
           L +     +     ++QCG       C  NLCCS++G+CG TD YC TGC+S  C  ST 
Sbjct: 14  LFLFTLTIMAKTVFSQQCGTTG----CAANLCCSRYGYCGTTDAYCGTGCRSGPCSSSTT 73

Query: 66  STPPSSGGG----------SIGGIISEGFYNQMLKYSRDARCPSNGFYTYNAFITAAQSF 125
             PP+  GG          +I  +++  F++ ++    +  CP+ GFYT  AFI AAQSF
Sbjct: 74  PIPPTPSGGAGGLNADPRDTIENVVTPAFFDGIMSKVGNG-CPAKGFYTRQAFIAAAQSF 133

Query: 126 PGFGTTGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPNQQ 185
             +  T      KRE+AA   Q SHE             +  +C+  EI +  YC+P+  
Sbjct: 134 DAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGKYCSPSTA 189

Query: 186 WPCVAGQKYYGRGPIQLT 193
           +PC  G+ YYGRGPIQ+T
Sbjct: 194 YPCTPGKDYYGRGPIQIT 189

The following BLAST results are available for this feature:

BLAST of Lsi01G005440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19171	1.1e-72	64.95	Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3	[more]
Q09023	2.1e-71	64.95	Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1	[more]
P08252	3.9e-70	63.40	Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2	[more]
P36907	6.6e-70	64.40	Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P93680	1.1e-69	64.36	Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2	[more]

BLAST of Lsi01G005440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GD14	5.4e-99	87.50	endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1	[more]
A0A6J1ILX6	7.0e-99	87.50	chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1	[more]
A0A1S3B2Y3	6.6e-97	86.98	chitinase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485182 PE=4 SV=1	[more]
A0A6J1GD34	5.6e-96	85.57	endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1	[more]
A0A5A7SHJ5	3.1e-94	84.38	Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]

BLAST of Lsi01G005440 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603867.1	1.7e-99	88.02	hypothetical protein SDJN03_04476, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038883175.1	6.5e-99	75.85	endochitinase-like [Benincasa hispida]	[more]
XP_023544980.1	8.5e-99	87.50	endochitinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949791.1	1.1e-98	87.50	endochitinase-like [Cucurbita moschata]	[more]
XP_022977305.1	1.5e-98	87.50	chitinase 1-like [Cucurbita maxima]	[more]

BLAST of Lsi01G005440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12500.1	7.8e-74	64.95	basic chitinase	[more]
AT4G01700.1	1.2e-34	53.33	Chitinase family protein	[more]
AT1G02360.1	1.0e-33	50.41	Chitinase family protein	[more]
AT2G43610.1	1.1e-30	39.70	Chitinase family protein	[more]
AT2G43620.1	6.9e-30	36.36	Chitinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	PRINTS	PR00451	CHITINBINDNG	coord: 38..45 score: 80.67 coord: 45..52 score: 57.5 coord: 30..38 score: 66.37
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 22..59 e-value: 3.0E-17 score: 73.3
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 22..59 e-value: 1.2E-15 score: 57.6
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 20..61 score: 11.015115
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 20..60 e-value: 4.0E-19 score: 70.4
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 22..65
None	No IPR available	GENE3D	1.10.530.10		coord: 61..185 e-value: 4.4E-42 score: 146.1
None	No IPR available	PIRSR	PIRSR001060-2	PIRSR001060-2	coord: 2..192 e-value: 4.5E-77 score: 257.5
None	No IPR available	PANTHER	PTHR22595:SF171	CHITINASE (CLASS IB) / HEVEIN	coord: 5..192
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 5..192
None	No IPR available	CDD	cd06921	ChtBD1_GH19_hevein	coord: 21..59 e-value: 1.98207E-17 score: 70.2108
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 81..192 e-value: 2.00159E-43 score: 142.569
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 78..192 e-value: 6.8E-55 score: 186.4
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 96..118
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 1..193 e-value: 4.8E-68 score: 227.7
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 32..51
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 75..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G005440.1	Lsi01G005440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0006952	defense response
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding