Lsi01G005420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G005420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionChitinase
Locationchr01: 4396179 .. 4397998 (-)
RNA-Seq ExpressionLsi01G005420
SyntenyLsi01G005420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGTTTTTGATTTAACCATGTCGATAAAATCTAGAGAAGTGTAATAATACTTTAAAATGTGATACGAATATCTCGAGTTAAAGTTCATCAATATTAAATTGACCAGGTGATAAGCATGGAAAATAAATTAAAAATAACTAAATTTTTTCATATGCAATAACAATAAAAATATTATTTTATATGAAGTTTTCGGATTAAAATAAGATATTAAAAGATTGGAACGTGTGGTGTATTGGCGGGAATTGGACCATATTATTTATTTATGATACACGTGAAATAATTTTATATTATATTATTTTTCCTCTTCTTTTAAAGCGTGAGTGAGATGCTATAAATTAAATGATAAAAAAAAAATTAATATTTATGAGTAGTGTGGTATACAAATAAATAGGGAAGGAAAGAAATTGCATAGTCCAAAGATTTAATTTGAGAAATGAAGGTTCACTCATTTTTTAGTTTGTTATTATTTTTGGGTGTGTTATGGAGAGTGGGTGCAGCCGCAGGTCCGGACGATGTGGGACGGCTCATCAACGCCACAATATTCAATCAAATGTTCAAATATCAAAAGGATCCACAATGTCCTAGCCAAGGGTTCTATAATTACAAAGCTTTTCTAGCTGCTGCTCGTTCTTTTACTAAGCTAGGGTTCGCCACAACAGGAAAACTTGCTACTCGAAAGAGGGAGTTGCTTGCTTTCTTTGCCCAAACTTCTCACCAAACCACCGGTTAGTATGTGAATGATAGTCTTTTATCGTTGAGAATGTATGAGGAACTTCTTGGTCCTTAGCATACATTGATACCAGTTGAGCTAAACTCTTTTTGACGAGAGACAAATATCTTGAATTTATATTTGTTAGATGATATAATATTAAATTTACTTTTACTCATCAGTTTAAACTTTTAGATCAACCAAGAATTTGATAATATTAACTCATTATTAAAAAGCATGCATGTTTCTAAATTTGCATTCTGCCTACAAAGTTAATTAATATGAGACTGATATGTTGTAGTGACATGATTGAAATTTTTTTTAATAGAATTTTAATTTGTTCTAAAGATGGTTAACAATAAAGAAATTACTTTGACCATGTGTAGGAGGATGGCTAACTGCACCAGATGGTCCACTTTTTTGGGGATATTGTCATATAAGGGAAACCACTAAGGATTCGTATTGCAAAGCTGATCCAAAGTGGCCTTGCGCTCCACGTCAGAAATATTACGGTCGTGGACCAATGCAACTCAGATAGTATATTATTTTTCTCTATCTCGTCCTTACAACTTTGGTTTATATCAAGAAAGTTGTTTGTAAACTAATATTGGTATTTTGCCTTGTTTTTTTTATTGAATTTAATTAATTAGCAACCATCACTACGGAGAAGCTAGTAAAGCATTGGGATATGATTTGTTGAAGAACCCAGATTTAGTAGCTAGAGACGCAGTAATATCATTCAAGACAGCGATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCATGTCATGATGTTATGGTTGGCAAATGGCGACCAACAGAAGCTGACAAAGCTGCTAAAAGAGTCCCTGGTTATGGTGTCGTCACAAACATCATCAATGGCGGAGAAGAATGCGGGCATGGTGCTGATACAGGAGTTGCTGATAGATTTGGATTTTATGAAAGATATTGTAAAATGTTGGATGTCAATCCAGGAAAACACTTGGAATGCTTTTTTCAAGAACCTTTCACTCGAATGTAATACAATTCTACTTTTTTGTTTCAAAATTTGGAAGGCTTGTAATAATATGGAATAAAAGTATTTGCAAAATGGTCTCTTGCT

mRNA sequence

ATGCATGTTCACTCATTTTTTAGTTTGTTATTATTTTTGGGTGTGTTATGGAGAGTGGGTGCAGCCGCAGGTCCGGACGATGTGGGACGGCTCATCAACGCCACAATATTCAATCAAATGTTCAAATATCAAAAGGATCCACAATGTCCTAGCCAAGGGTTCTATAATTACAAAGCTTTTCTAGCTGCTGCTCGTTCTTTTACTAAGCTAGGGTTCGCCACAACAGGAAAACTTGCTACTCGAAAGAGGGAAATTTTAATTTGTTCTAAAGATGGAGGATGGCTAACTGCACCAGATGGTCCACTTTTTTGGGGATATTGTCATATAAGGGAAACCACTAAGGATTCGTATTGCAAAGCTGATCCAAACAACCATCACTACGGAGAAGCTAGTAAAGCATTGGGATATGATTTGTTGAAGAACCCAGATTTAGTAGCTAGAGACGCAGTAATATCATTCAAGACAGCGATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCATGTCATGATGTTATGGTTGGCAAATGGCGACCAACAGAAGCTGACAAAGCTGCTAAAAGAGTCCCTGGTTATGGTGTCGTCACAAACATCATCAATGGCGGAGAAGAATGCGGGCATGGTGCTGATACAGGAGTTGCTGATAGATTTGGATTTTATGAAAGATATTGTAAAATGTTGGATGTCAATCCAGGAAAACACTTGGAATGCTTTTTTCAAGAACCTTTCACTCGAATGTAATACAATTCTACTTTTTTGTTTCAAAATTTGGAAGGCTTGTAATAATATGGAATAAAAGTATTTGCAAAATGGTCTCTTGCT

Coding sequence (CDS)

ATGCATGTTCACTCATTTTTTAGTTTGTTATTATTTTTGGGTGTGTTATGGAGAGTGGGTGCAGCCGCAGGTCCGGACGATGTGGGACGGCTCATCAACGCCACAATATTCAATCAAATGTTCAAATATCAAAAGGATCCACAATGTCCTAGCCAAGGGTTCTATAATTACAAAGCTTTTCTAGCTGCTGCTCGTTCTTTTACTAAGCTAGGGTTCGCCACAACAGGAAAACTTGCTACTCGAAAGAGGGAAATTTTAATTTGTTCTAAAGATGGAGGATGGCTAACTGCACCAGATGGTCCACTTTTTTGGGGATATTGTCATATAAGGGAAACCACTAAGGATTCGTATTGCAAAGCTGATCCAAACAACCATCACTACGGAGAAGCTAGTAAAGCATTGGGATATGATTTGTTGAAGAACCCAGATTTAGTAGCTAGAGACGCAGTAATATCATTCAAGACAGCGATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCATGTCATGATGTTATGGTTGGCAAATGGCGACCAACAGAAGCTGACAAAGCTGCTAAAAGAGTCCCTGGTTATGGTGTCGTCACAAACATCATCAATGGCGGAGAAGAATGCGGGCATGGTGCTGATACAGGAGTTGCTGATAGATTTGGATTTTATGAAAGATATTGTAAAATGTTGGATGTCAATCCAGGAAAACACTTGGAATGCTTTTTTCAAGAACCTTTCACTCGAATGTAA

Protein sequence

MHVHSFFSLLLFLGVLWRVGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLATRKREILICSKDGGWLTAPDGPLFWGYCHIRETTKDSYCKADPNNHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM
Homology
BLAST of Lsi01G005420 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-69
Identity = 128/252 (50.79%), Postives = 160/252 (63.49%), Query Frame = 0

Query: 20  GAAAGP-DDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKL 79
           G   GP  D+  +I+ + F+ M K++ D  CP++GFY Y AF+ AA+SF   GF TTG  
Sbjct: 62  GTPPGPTGDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFP--GFGTTGDT 121

Query: 80  ATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDS-YCKADPN-------- 139
           ATRK+EI            GGW TAPDGP  WGYC  +E    S YC             
Sbjct: 122 ATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGKS 181

Query: 140 -----------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHD 199
                      N++YG+  +A+G DLL NPDLV+ D VI+FK AIWFWMTPQ  KPSCH 
Sbjct: 182 YYGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHA 241

Query: 200 VMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNP 245
           V+VG+W+P++AD+AA RVPGYGV+TNIINGG ECG G D  VADR GFY+RYC +L VNP
Sbjct: 242 VIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNP 301

BLAST of Lsi01G005420 vs. ExPASy Swiss-Prot
Match: P06215 (Endochitinase OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.5e-69
Identity = 122/250 (48.80%), Postives = 157/250 (62.80%), Query Frame = 0

Query: 20  GAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLA 79
           G +  P D+  LI+ + F+QM K++ D  CP++GFY Y AF+AAA+++    F  TG  A
Sbjct: 68  GPSPAPTDLSALISRSTFDQMLKHRNDGACPAKGFYTYDAFIAAAKAYP--SFGNTGDTA 127

Query: 80  TRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDSYCKADPN---------- 139
           TRKREI            GGW TAPDGP  WGYC +RE    +YC A P           
Sbjct: 128 TRKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSTYCSATPQFPCAPGQQYY 187

Query: 140 ---------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVM 199
                    N++YG+  +A+G DLL  PDLVA D+VISFK+A+WFWMT Q  KPS HDV+
Sbjct: 188 GRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPKPSSHDVI 247

Query: 200 VGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNPGK 245
             +W P+ AD AA+R+PGYG VTNIINGG ECG G D+ V DR GF++RYC +L V  G 
Sbjct: 248 TSRWTPSSADVAARRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFFKRYCDLLGVGYGN 307

BLAST of Lsi01G005420 vs. ExPASy Swiss-Prot
Match: Q39799 (Endochitinase 1 OS=Gossypium hirsutum OX=3635 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.5e-69
Identity = 128/253 (50.59%), Postives = 160/253 (63.24%), Query Frame = 0

Query: 20  GAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLA 79
           G A GP  +  LI+   FNQM  ++ D  CP++GFY Y AF+AAARSF    FATTG  A
Sbjct: 66  GPAPGPGGLTNLISRETFNQMLLHRNDGACPARGFYTYDAFIAAARSFP--AFATTGDQA 125

Query: 80  TRKREILI--------CSKDGGWLTAPDGPLFWGYCHIRE-TTKDSYCKADPN------- 139
           TRKREI           +   GW  APDGP  WGYC+ RE     SYC +DPN       
Sbjct: 126 TRKREIAAFLAQTSHETTGGAGW-AAPDGPYAWGYCYNRELNPPSSYCASDPNYPCAPGK 185

Query: 140 ------------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCH 199
                       N++YG+  +A+G DLL NPDL++ D  ISFK+A WFWMTPQ  KPSCH
Sbjct: 186 QYFGRGPMQLSWNYNYGQCGRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPKPSCH 245

Query: 200 DVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVN 245
           +V++G W P+ +D+AA RVPGYGV+TNIINGG ECG G +  V DR GFY+RYC +L V+
Sbjct: 246 NVIIGAWSPSSSDRAAGRVPGYGVITNIINGGLECGKGWNAQVEDRIGFYKRYCDILGVS 305

BLAST of Lsi01G005420 vs. ExPASy Swiss-Prot
Match: P24091 (Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.5e-69
Identity = 123/247 (49.80%), Postives = 159/247 (64.37%), Query Frame = 0

Query: 24  GPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLATRKR 83
           G  D+G +I++++F+QM K++ D  C  +GFY+Y AF+ AARSF   GF T+G    RKR
Sbjct: 71  GGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFP--GFGTSGDTTARKR 130

Query: 84  EILIC------SKDGGWLTAPDGPLFWGYCHIRET-TKDSYCKAD--------------- 143
           EI            GGW TAPDGP  WGYC +RE  +   YC                  
Sbjct: 131 EIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRG 190

Query: 144 ----PNNHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVMVGK 203
                +N++YG   +A+G DLL NPDLVA D VISFK+A+WFWMTPQ  KPSCHDV++G+
Sbjct: 191 PIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGR 250

Query: 204 WRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNPGKHLE 245
           W+P+ AD+AA R+PG+GV+TNIINGG ECG G D+ V DR GFY RYC +L V+PG +L+
Sbjct: 251 WQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLD 310

BLAST of Lsi01G005420 vs. ExPASy Swiss-Prot
Match: P36361 (Endochitinase CH5B OS=Phaseolus vulgaris OX=3885 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.2e-69
Identity = 121/250 (48.40%), Postives = 157/250 (62.80%), Query Frame = 0

Query: 20  GAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLA 79
           G +  P D+  LI+ + F+Q+ K++ D  CP++GFY Y AF+AAA+++    F  TG  A
Sbjct: 67  GPSPAPTDLSALISRSTFDQVLKHRNDGACPAKGFYTYDAFIAAAKAYP--SFGNTGDTA 126

Query: 80  TRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDSYCKADPN---------- 139
           TRKREI            GGW TAPDGP  WGYC +RE    +YC A P           
Sbjct: 127 TRKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSAYCSATPQFPCAPGQQYY 186

Query: 140 ---------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVM 199
                    N++YG+  +A+G DLL  PDLVA D+VISFK+A+WFWMT Q  KPS HDV+
Sbjct: 187 GRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPKPSSHDVI 246

Query: 200 VGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNPGK 245
             +W P+ AD AA+R+PGYG VTNIINGG ECG G D+ V DR GF++RYC +L V  G 
Sbjct: 247 TSRWTPSSADVAARRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFFKRYCDLLGVGYGN 306

BLAST of Lsi01G005420 vs. ExPASy TrEMBL
Match: A0A0A0KHG3 (Glyco_hydro_19_cat domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G509030 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.9e-111
Identity = 201/273 (73.63%), Postives = 217/273 (79.49%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWR-VGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKA 60
           M ++S  SLL+ LGVLWR VG A+   DVGRLIN T FN MFKYQKDPQC SQGFY+Y+A
Sbjct: 1   MKLYSLLSLLI-LGVLWRSVGVASSSSDVGRLINVTTFNAMFKYQKDPQCSSQGFYSYQA 60

Query: 61  FLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETT 120
           FLAAARSF KLGFATTGKLATRKRE+L           GGWLTAPDGPLFWGYCHIRETT
Sbjct: 61  FLAAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPDGPLFWGYCHIRETT 120

Query: 121 KDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISFK 180
           +DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SFK
Sbjct: 121 EDSYCKADPKWPCAKGQKYYGRGPMQLQGNQNYGQAGKALGLDLLKNPDLVAKDPVVSFK 180

Query: 181 TAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGV 240
           TAIWFWMTPQG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T V
Sbjct: 181 TAIWFWMTPQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGRECGSGANTDV 240

Query: 241 ADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           ADRFGFY RYCKML VNPGKHL+CFFQEPFTRM
Sbjct: 241 ADRFGFYVRYCKMLGVNPGKHLDCFFQEPFTRM 271

BLAST of Lsi01G005420 vs. ExPASy TrEMBL
Match: Q9LE03 (Chitinase OS=Cucumis melo OX=3656 GN=chi2 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 5.6e-109
Identity = 198/274 (72.26%), Postives = 216/274 (78.83%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWRVG--AAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYK 60
           M ++S  S LLFLGVLWR    A++  DDVGRLIN T FN MFKYQKDPQCPSQGFY+Y+
Sbjct: 1   MKLYSLLS-LLFLGVLWRSEGVASSSNDDVGRLINVTTFNAMFKYQKDPQCPSQGFYSYQ 60

Query: 61  AFLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRET 120
           AFL AARSF KLGFATTGKLATRKRE+L           GGWLTAPDGPLFWGYCHIRE+
Sbjct: 61  AFLTAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPDGPLFWGYCHIRES 120

Query: 121 TKDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISF 180
           T+DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SF
Sbjct: 121 TEDSYCKADPKWPCAKGQKYYGRGPMQLKGNQNYGQAGKALGLDLLKNPDLVAKDPVVSF 180

Query: 181 KTAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTG 240
           KTAIWFWMT QG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T 
Sbjct: 181 KTAIWFWMTAQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGSECGSGANTD 240

Query: 241 VADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           VADRFGFY RYCKML VNPGKHL+CFFQ+PFTRM
Sbjct: 241 VADRFGFYVRYCKMLGVNPGKHLDCFFQQPFTRM 272

BLAST of Lsi01G005420 vs. ExPASy TrEMBL
Match: A0A5A7SLP3 (Chitinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00700 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.8e-108
Identity = 196/274 (71.53%), Postives = 215/274 (78.47%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWRVG--AAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYK 60
           M ++S  S LLFLGVLWR    A++  DDVGRLIN T FN MFKYQKDPQCPSQGFY+Y+
Sbjct: 1   MKLYSLLS-LLFLGVLWRSEGVASSSNDDVGRLINVTTFNAMFKYQKDPQCPSQGFYSYQ 60

Query: 61  AFLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRET 120
           AFL AARSF KLGFATTGKLATRKRE+L           GGWLTAP+GPLFWGYCHIRE+
Sbjct: 61  AFLTAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPEGPLFWGYCHIRES 120

Query: 121 TKDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISF 180
           T+DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SF
Sbjct: 121 TEDSYCKADPKWPCAKGQKYYGRGPMQLKGNQNYGQAGKALGLDLLKNPDLVAKDPVVSF 180

Query: 181 KTAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTG 240
           KTAIWFWMT QG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T 
Sbjct: 181 KTAIWFWMTAQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGSECGSGANTD 240

Query: 241 VADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           VADRFGFY RYCKML  NPGKHL+CFFQ+PFTRM
Sbjct: 241 VADRFGFYVRYCKMLGANPGKHLDCFFQQPFTRM 272

BLAST of Lsi01G005420 vs. ExPASy TrEMBL
Match: A0A6J1BUG0 (LOW QUALITY PROTEIN: endochitinase-like OS=Momordica charantia OX=3673 GN=LOC111005462 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.8e-98
Identity = 174/268 (64.93%), Postives = 203/268 (75.75%), Query Frame = 0

Query: 6   FFSLLL--FLGVLWRVGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAA 65
           FF+LLL  F G+   V A++  DDVG+L+NATIF++MF+ +KDPQC S+ FY Y AFLAA
Sbjct: 295 FFALLLRVFAGL---VHASSHKDDVGKLVNATIFDEMFERRKDPQCSSENFYTYNAFLAA 354

Query: 66  ARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDSY 125
           A SF + GF TTG + TRKRE+            GGWLTAP G L WGYCHI+ETT+DSY
Sbjct: 355 AHSFGRFGFGTTGSVNTRKRELAAFLAQTSHQTTGGWLTAPGGILAWGYCHIKETTQDSY 414

Query: 126 CKADPN-------------------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIW 185
           CK DP                    N +YG+A KALG DLLKNPDLVA DA++SFKTAIW
Sbjct: 415 CKPDPKWPCVRGQKYYGRGPMQLKYNSNYGQAGKALGLDLLKNPDLVATDAILSFKTAIW 474

Query: 186 FWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRF 245
           FWMTPQG KPSCHDV++GKW+P++ADKAAKRVPGYGV+TNIINGGEECGHGADT VADRF
Sbjct: 475 FWMTPQGIKPSCHDVIIGKWKPSDADKAAKRVPGYGVITNIINGGEECGHGADTEVADRF 534

Query: 246 GFYERYCKMLDVNPGKHLECFFQEPFTR 247
           GFYE+YCKML VNPGKHL C+FQ+PF R
Sbjct: 535 GFYEKYCKMLGVNPGKHLACYFQDPFVR 559

BLAST of Lsi01G005420 vs. ExPASy TrEMBL
Match: A0A1S3B3G7 (endochitinase-like OS=Cucumis melo OX=3656 GN=LOC103485363 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.3e-86
Identity = 156/267 (58.43%), Postives = 189/267 (70.79%), Query Frame = 0

Query: 7   FSLLLFLGVLWRVGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARS 66
           FSLL   GV     +    DDVG LI  T+FN+MFK+QKDP+C SQGFY+Y+AF+ AAR 
Sbjct: 11  FSLLWGRGVPSTTPSPNEGDDVGNLITPTMFNEMFKHQKDPECESQGFYSYQAFITAARY 70

Query: 67  FTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDSYCKA 126
           F++ GFATTGK + RKRE+            GGW+  PDGP FWGYC I+ETT DSYC  
Sbjct: 71  FSRFGFATTGKPSVRKREVAAFLAQTSHQTTGGWVAVPDGPTFWGYCRIKETTNDSYC-- 130

Query: 127 DPN-------------------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWM 186
           DP                    N +Y +A KALG DLLKNPDL+A D V+SFKTAIW+WM
Sbjct: 131 DPKWPCAPAQKYYGRGPMQLQYNFNYMKAGKALGLDLLKNPDLLATDPVVSFKTAIWYWM 190

Query: 187 TPQG-NKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGF 246
           T +G N PSCHDV++GKWRPT+AD AA+R+PG+GV+TNIINGGEECGH ADT V DRF F
Sbjct: 191 TSRGLNIPSCHDVIIGKWRPTKADIAARRLPGFGVITNIINGGEECGHDADTSVLDRFEF 250

Query: 247 YERYCKMLDVNPGKHLECFFQEPFTRM 248
           + RYCKM+ +  GKH++CFFQ+PFTRM
Sbjct: 251 FMRYCKMMGIKTGKHIDCFFQQPFTRM 275

BLAST of Lsi01G005420 vs. NCBI nr
Match: XP_038881284.1 (endochitinase-like [Benincasa hispida])

HSP 1 Score: 432.6 bits (1111), Expect = 2.3e-117
Identity = 206/270 (76.30%), Postives = 219/270 (81.11%), Query Frame = 0

Query: 3   VHSFFSLLLFLGVLWRVGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLA 62
           ++S  SLLL L VLW VGAA G  DVGRLINAT+FN+MFKYQKDPQCPSQGFY+Y+AFLA
Sbjct: 4   IYSLLSLLL-LDVLWGVGAAEGSKDVGRLINATMFNEMFKYQKDPQCPSQGFYSYQAFLA 63

Query: 63  AARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDS 122
           AA SF KLGFATTGKL+TRKRE+L           GGW TAPDGP FWGYCHIRETTKDS
Sbjct: 64  AASSFAKLGFATTGKLSTRKREVLAFLAQTSHQTTGGWPTAPDGPFFWGYCHIRETTKDS 123

Query: 123 YCKADPN-------------------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAI 182
           YCKAD                     NH+YGEA K LG DLLKNPDLVA+DAV+SFKTAI
Sbjct: 124 YCKADSKWPCAKGQKYYGRGPMQLRYNHNYGEAGKMLGLDLLKNPDLVAKDAVVSFKTAI 183

Query: 183 WFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADR 242
           WFWMTPQ NKPSCHDVMVGKWRPTE DKAAKRVPG+GVVTNIINGGEECGHGADTGVADR
Sbjct: 184 WFWMTPQENKPSCHDVMVGKWRPTEIDKAAKRVPGFGVVTNIINGGEECGHGADTGVADR 243

Query: 243 FGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           FGFYERYC ML +NPGKHLECFFQEPFTRM
Sbjct: 244 FGFYERYCNMLGINPGKHLECFFQEPFTRM 272

BLAST of Lsi01G005420 vs. NCBI nr
Match: XP_004134831.1 (endochitinase [Cucumis sativus] >KGN48983.1 hypothetical protein Csa_004399 [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 1.6e-110
Identity = 201/273 (73.63%), Postives = 217/273 (79.49%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWR-VGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKA 60
           M ++S  SLL+ LGVLWR VG A+   DVGRLIN T FN MFKYQKDPQC SQGFY+Y+A
Sbjct: 1   MKLYSLLSLLI-LGVLWRSVGVASSSSDVGRLINVTTFNAMFKYQKDPQCSSQGFYSYQA 60

Query: 61  FLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETT 120
           FLAAARSF KLGFATTGKLATRKRE+L           GGWLTAPDGPLFWGYCHIRETT
Sbjct: 61  FLAAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPDGPLFWGYCHIRETT 120

Query: 121 KDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISFK 180
           +DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SFK
Sbjct: 121 EDSYCKADPKWPCAKGQKYYGRGPMQLQGNQNYGQAGKALGLDLLKNPDLVAKDPVVSFK 180

Query: 181 TAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGV 240
           TAIWFWMTPQG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T V
Sbjct: 181 TAIWFWMTPQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGRECGSGANTDV 240

Query: 241 ADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           ADRFGFY RYCKML VNPGKHL+CFFQEPFTRM
Sbjct: 241 ADRFGFYVRYCKMLGVNPGKHLDCFFQEPFTRM 271

BLAST of Lsi01G005420 vs. NCBI nr
Match: AAF64475.1 (chitinase 2 [Cucumis melo] >AAF69836.1 chitinase [Cucumis melo])

HSP 1 Score: 403.7 bits (1036), Expect = 1.2e-108
Identity = 198/274 (72.26%), Postives = 216/274 (78.83%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWRVG--AAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYK 60
           M ++S  S LLFLGVLWR    A++  DDVGRLIN T FN MFKYQKDPQCPSQGFY+Y+
Sbjct: 1   MKLYSLLS-LLFLGVLWRSEGVASSSNDDVGRLINVTTFNAMFKYQKDPQCPSQGFYSYQ 60

Query: 61  AFLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRET 120
           AFL AARSF KLGFATTGKLATRKRE+L           GGWLTAPDGPLFWGYCHIRE+
Sbjct: 61  AFLTAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPDGPLFWGYCHIRES 120

Query: 121 TKDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISF 180
           T+DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SF
Sbjct: 121 TEDSYCKADPKWPCAKGQKYYGRGPMQLKGNQNYGQAGKALGLDLLKNPDLVAKDPVVSF 180

Query: 181 KTAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTG 240
           KTAIWFWMT QG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T 
Sbjct: 181 KTAIWFWMTAQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGSECGSGANTD 240

Query: 241 VADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           VADRFGFY RYCKML VNPGKHL+CFFQ+PFTRM
Sbjct: 241 VADRFGFYVRYCKMLGVNPGKHLDCFFQQPFTRM 272

BLAST of Lsi01G005420 vs. NCBI nr
Match: KAA0025617.1 (chitinase 2 [Cucumis melo var. makuwa] >TYK12492.1 chitinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 400.6 bits (1028), Expect = 9.9e-108
Identity = 196/274 (71.53%), Postives = 215/274 (78.47%), Query Frame = 0

Query: 1   MHVHSFFSLLLFLGVLWRVG--AAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYK 60
           M ++S  S LLFLGVLWR    A++  DDVGRLIN T FN MFKYQKDPQCPSQGFY+Y+
Sbjct: 1   MKLYSLLS-LLFLGVLWRSEGVASSSNDDVGRLINVTTFNAMFKYQKDPQCPSQGFYSYQ 60

Query: 61  AFLAAARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRET 120
           AFL AARSF KLGFATTGKLATRKRE+L           GGWLTAP+GPLFWGYCHIRE+
Sbjct: 61  AFLTAARSFGKLGFATTGKLATRKRELLAFLAQTSHQTTGGWLTAPEGPLFWGYCHIRES 120

Query: 121 TKDSYCKADP-------------------NNHHYGEASKALGYDLLKNPDLVARDAVISF 180
           T+DSYCKADP                    N +YG+A KALG DLLKNPDLVA+D V+SF
Sbjct: 121 TEDSYCKADPKWPCAKGQKYYGRGPMQLKGNQNYGQAGKALGLDLLKNPDLVAKDPVVSF 180

Query: 181 KTAIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTG 240
           KTAIWFWMT QG KPSCHDVMVGKW+PTEADKAAKRVPGYGVV+NII GG ECG GA+T 
Sbjct: 181 KTAIWFWMTAQGIKPSCHDVMVGKWKPTEADKAAKRVPGYGVVSNII-GGSECGSGANTD 240

Query: 241 VADRFGFYERYCKMLDVNPGKHLECFFQEPFTRM 248
           VADRFGFY RYCKML  NPGKHL+CFFQ+PFTRM
Sbjct: 241 VADRFGFYVRYCKMLGANPGKHLDCFFQQPFTRM 272

BLAST of Lsi01G005420 vs. NCBI nr
Match: XP_022132657.1 (LOW QUALITY PROTEIN: endochitinase-like [Momordica charantia])

HSP 1 Score: 367.1 bits (941), Expect = 1.2e-97
Identity = 174/268 (64.93%), Postives = 203/268 (75.75%), Query Frame = 0

Query: 6   FFSLLL--FLGVLWRVGAAAGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAA 65
           FF+LLL  F G+   V A++  DDVG+L+NATIF++MF+ +KDPQC S+ FY Y AFLAA
Sbjct: 295 FFALLLRVFAGL---VHASSHKDDVGKLVNATIFDEMFERRKDPQCSSENFYTYNAFLAA 354

Query: 66  ARSFTKLGFATTGKLATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDSY 125
           A SF + GF TTG + TRKRE+            GGWLTAP G L WGYCHI+ETT+DSY
Sbjct: 355 AHSFGRFGFGTTGSVNTRKRELAAFLAQTSHQTTGGWLTAPGGILAWGYCHIKETTQDSY 414

Query: 126 CKADPN-------------------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIW 185
           CK DP                    N +YG+A KALG DLLKNPDLVA DA++SFKTAIW
Sbjct: 415 CKPDPKWPCVRGQKYYGRGPMQLKYNSNYGQAGKALGLDLLKNPDLVATDAILSFKTAIW 474

Query: 186 FWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRF 245
           FWMTPQG KPSCHDV++GKW+P++ADKAAKRVPGYGV+TNIINGGEECGHGADT VADRF
Sbjct: 475 FWMTPQGIKPSCHDVIIGKWKPSDADKAAKRVPGYGVITNIINGGEECGHGADTEVADRF 534

Query: 246 GFYERYCKMLDVNPGKHLECFFQEPFTR 247
           GFYE+YCKML VNPGKHL C+FQ+PF R
Sbjct: 535 GFYEKYCKMLGVNPGKHLACYFQDPFVR 559

BLAST of Lsi01G005420 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 258.8 bits (660), Expect = 4.3e-69
Identity = 125/252 (49.60%), Postives = 159/252 (63.10%), Query Frame = 0

Query: 20  GAAAGP-DDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKL 79
           G   GP  D+  +I+++ F+ M K++ D  CP++GFY Y AF+ AA+SF   GF TTG  
Sbjct: 77  GTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFP--GFGTTGDT 136

Query: 80  ATRKREILIC------SKDGGWLTAPDGPLFWGYCHIRETTKDS-YCKADPN-------- 139
           ATRK+E+            GGW TAPDGP  WGYC  +E    S YC+            
Sbjct: 137 ATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKR 196

Query: 140 -----------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHD 199
                      N++YG   +A+G DLL NPDLVA DAVI+FK AIWFWMT Q  KPSCH 
Sbjct: 197 YYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHA 256

Query: 200 VMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNP 245
           V+ G+W+P++AD+AA R+PGYGV+TNIINGG ECG G D  VADR GFY+RYC +  VNP
Sbjct: 257 VIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNP 316

BLAST of Lsi01G005420 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.4e-59
Identity = 110/248 (44.35%), Postives = 154/248 (62.10%), Query Frame = 0

Query: 25  PDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLATRKRE 84
           P  +  L+  T+++Q+F ++ +  CP++GFY Y+AF+ A RSF K  F + G   TR+RE
Sbjct: 35  PAPIMSLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPK--FGSVGNFWTRRRE 94

Query: 85  I------LICSKDGGWLTAPDGPLFWGYCHIRETTKDS-YCKADPN-------------- 144
           +      +     GGW TAPDGP  WG C   E +  S YC A                 
Sbjct: 95  VAAFLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRG 154

Query: 145 ------NHHYGEASKALGYDLLKNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVMVGK 204
                 N++YG+A +ALG+D L+NP+LVA ++V++FKTA+WFWMT Q  KPSCH+VMV +
Sbjct: 155 PIQLSWNYNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNR 214

Query: 205 WRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKMLDVNPGKHLE 246
           +RPT+AD+AA R  GYG+VTNIINGG ECG   D  V DR G+++RY ++  V  G +L+
Sbjct: 215 YRPTKADRAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLD 274

BLAST of Lsi01G005420 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 221.9 bits (564), Expect = 5.8e-58
Identity = 111/269 (41.26%), Postives = 158/269 (58.74%), Query Frame = 0

Query: 4   HSFFSLLLFLGVLWRVGAA-AGPDDVGRLINATIFNQMFKYQKDPQCPSQGFYNYKAFLA 63
           HSF  L  FL + + + +A      + RL+   ++N++F ++ +  CP+ GFY Y++F+ 
Sbjct: 5   HSFLLLCFFLSISYLLSSAQTEATSIERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQ 64

Query: 64  AARSFTKLGFATTGKLATRKREI------LICSKDGGWLTAPDGPLFWGYCHIRETTKDS 123
           A R F +  F + G   T++ E+      +     GGW TAPDGP  WG C   E +  S
Sbjct: 65  ATRRFPR--FGSVGSPVTQRLEVAAFLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQS 124

Query: 124 -YCKAD-------PN-------------NHHYGEASKALGYDLLKNPDLVARDAVISFKT 183
            YC +        PN             N++YG A +ALG+D L+NP+ V+ ++VI+F+T
Sbjct: 125 TYCDSSDTQWPCFPNKTYQGRGPIQLSWNYNYGPAGRALGFDGLRNPETVSNNSVIAFQT 184

Query: 184 AIWFWMTPQGNKPSCHDVMVGKWRPTEADKAAKRVPGYGVVTNIINGGEECGHGADTGVA 243
           A+WFWMTPQ  KPSCHDVM+GK+RPT AD AA R  G+G+ TNIINGG ECG   D  V 
Sbjct: 185 ALWFWMTPQSPKPSCHDVMIGKYRPTAADLAANRTGGFGLTTNIINGGLECGIPGDGRVN 244

Query: 244 DRFGFYERYCKMLDVNPGKHLECFFQEPF 245
           DR GF++RY  +  V  G +L+C  Q P+
Sbjct: 245 DRIGFFQRYTGLFKVATGPNLDCENQRPY 271

BLAST of Lsi01G005420 vs. TAIR 10
Match: AT3G16920.1 (chitinase-like protein 2 )

HSP 1 Score: 145.6 bits (366), Expect = 5.3e-35
Identity = 82/244 (33.61%), Postives = 123/244 (50.41%), Query Frame = 0

Query: 37  FNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLATRKREIL-----ICSKD 96
           F  +F  +  P   + GF++Y++F+ AA  +  LGF T G+     +E+      + SK 
Sbjct: 92  FENLFSKRNSPVAHAVGFWDYRSFITAAAEYQPLGFGTAGEKLQGMKEVAAFLGHVGSKT 151

Query: 97  G-GWLTAPDGPLFWGYCHIRETTKDS-YC--------KADPN-------------NHHYG 156
             G+  A  GPL WG C+ +E + D  YC           P              N++YG
Sbjct: 152 SCGYGVATGGPLAWGLCYNKEMSPDQLYCDDYYKLTYPCTPGVSYHGRGALPVYWNYNYG 211

Query: 157 EASKALGYDLLKNPDLVARDAVISFKTAIWFWMT-PQGNKPSCHDVMVGKWRPTEADKAA 216
           +  +AL  DLL +P+ +  +A ++F+ AIW WMT P+ + PS HDV VGKW+PT+ D AA
Sbjct: 212 QTGEALKVDLLSHPEYLENNATLAFQAAIWRWMTPPKKHLPSAHDVFVGKWKPTKNDTAA 271

Query: 217 KRVPGYGVVTNIINGGEECGHGADTGVADRF-GFYERYCKMLDV-----NPGKHLECFFQ 246
           KR PG+G   N++ G + C  G D    +     Y  Y  ++ V      P + L C  Q
Sbjct: 272 KRTPGFGATINVLYGDQICNSGFDNDEMNNIVSHYLYYLDLIGVGREEAGPHEKLSCADQ 331

BLAST of Lsi01G005420 vs. TAIR 10
Match: AT1G05850.1 (Chitinase family protein )

HSP 1 Score: 139.4 bits (350), Expect = 3.8e-33
Identity = 80/242 (33.06%), Postives = 121/242 (50.00%), Query Frame = 0

Query: 37  FNQMFKYQKDPQCPSQGFYNYKAFLAAARSFTKLGFATTGKLATRKREIL-----ICSKD 96
           F Q+F  +  P   + GF++Y++F+ AA  F  LGF TTG     ++E+      + SK 
Sbjct: 69  FEQLFSKRNTPIAHAVGFWDYQSFITAAALFEPLGFGTTGGKLMGQKEMAAFLGHVASKT 128

Query: 97  G-GWLTAPDGPLFWGYCHIRE-TTKDSYC--------KADPN-------------NHHYG 156
             G+  A  GPL WG C+ RE +   SYC           P              N +YG
Sbjct: 129 SCGYGVATGGPLAWGLCYNREMSPMQSYCDESWKFKYPCSPGAEYYGRGALPIYWNFNYG 188

Query: 157 EASKALGYDLLKNPDLVARDAVISFKTAIWFWMTP-QGNKPSCHDVMVGKWRPTEADKAA 216
            A +AL  DLL +P+ + ++A ++F+ AIW WMTP +  +PS HD+ VG W+PT+ D  +
Sbjct: 189 AAGEALKADLLNHPEYIEQNATLAFQAAIWRWMTPIKRAQPSAHDIFVGNWKPTKNDTLS 248

Query: 217 KRVPGYGVVTNIINGGEECGHGADTGVADRFGFYERYCKML-----DVNPGKHLECFFQE 245
           KR P +G   N++ G   CG G+   + +    Y  +  ++     D  P   L C  Q+
Sbjct: 249 KRGPTFGSTMNVLYGEYTCGQGSIDPMNNIISHYLYFLDLMGIGREDAGPNDELSCAEQK 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q090231.1e-6950.79Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1[more]
P062152.5e-6948.80Endochitinase OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
Q397995.5e-6950.59Endochitinase 1 OS=Gossypium hirsutum OX=3635 PE=3 SV=1[more]
P240915.5e-6949.80Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1[more]
P363617.2e-6948.40Endochitinase CH5B OS=Phaseolus vulgaris OX=3885 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHG37.9e-11173.63Glyco_hydro_19_cat domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
Q9LE035.6e-10972.26Chitinase OS=Cucumis melo OX=3656 GN=chi2 PE=4 SV=1[more]
A0A5A7SLP34.8e-10871.53Chitinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00700 PE... [more]
A0A6J1BUG05.8e-9864.93LOW QUALITY PROTEIN: endochitinase-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3B3G72.3e-8658.43endochitinase-like OS=Cucumis melo OX=3656 GN=LOC103485363 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038881284.12.3e-11776.30endochitinase-like [Benincasa hispida][more]
XP_004134831.11.6e-11073.63endochitinase [Cucumis sativus] >KGN48983.1 hypothetical protein Csa_004399 [Cuc... [more]
AAF64475.11.2e-10872.26chitinase 2 [Cucumis melo] >AAF69836.1 chitinase [Cucumis melo][more]
KAA0025617.19.9e-10871.53chitinase 2 [Cucumis melo var. makuwa] >TYK12492.1 chitinase 2 [Cucumis melo var... [more]
XP_022132657.11.2e-9764.93LOW QUALITY PROTEIN: endochitinase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G12500.14.3e-6949.60basic chitinase [more]
AT4G01700.11.4e-5944.35Chitinase family protein [more]
AT1G02360.15.8e-5841.26Chitinase family protein [more]
AT3G16920.15.3e-3533.61chitinase-like protein 2 [more]
AT1G05850.13.8e-3333.06Chitinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.530.10coord: 31..238
e-value: 8.1E-74
score: 250.1
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 110..147
e-value: 8.1E-74
score: 250.1
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 22..244
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 22..244
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 32..238
e-value: 3.06352E-71
score: 215.757
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 12..245
e-value: 6.7E-67
score: 223.9
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 124..238
e-value: 2.6E-57
score: 194.3
coord: 32..120
e-value: 7.0E-21
score: 75.1
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 151..161
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 27..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G005420.1Lsi01G005420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0050832 defense response to fungus
cellular_component GO:0016020 membrane
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding