Lsi01G004640 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G004640
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr01: 3876937 .. 3877862 (+)
RNA-Seq Expression	Lsi01G004640
Synteny	Lsi01G004640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTCTTCTCAGTGTGATTGTACAGGAAGCCGCCAGGCGAGAAAGGGAGGCTAGGACCATTTGGGGATTTTTGACCGATCAAATGGACGGTGTCGATGGAGGAGGAAGGCGGCGGCCCACTTTAAAGCAACGGCTAGGATTTAAAGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGCCGACTGTGAGCGTTAGAGACGGCGGCGGTGACGATGACGACCGGAGAGTACCGGATGTGGAGGTGATGAACACGCGCCGGTTTCCGGAGGAGAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTTCAGTCGCCGGGACCGACCGGGATGAACTTGGCCACGGCTCTGGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAACGGAGGGAGGTTTTGTGGAATCTAATAACAATGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTGGTGGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAAAAAAGAGAGAAGAGGCGGGAAATGATTCGATGTGCTGCGTGTGCATGGGGAGGAAAAAGGGTGCGGCATTTATCCCATGTGGGCACACGTTTTGTAGGGTGTGTTCGAGGGAGCTGTGGCTAAATCGAGGATCTTGCCCGCTTTGTAACCGTCCGATTATTGAAATTCTTGATATATTCTGAAGGACCATGATGAGTACGATGCGGATGAGAACAGAGAATTGTTGGTGACGGGAAGGGGTAGTGCTTTTGCCTCAAGATTCGCAACAGACTGTTTTCATTGGACGGCACTGAACGATGGCCGTATCTCTGCGACATGTGGATGCGATGCGGGTGAGAAAACTTTTTCACGTCCGAGAAAAAAGTTTAATTAA

mRNA sequence

ATGAGTCTTCTCAGTGTGATTGTACAGGAAGCCGCCAGGCGAGAAAGGGAGGCTAGGACCATTTGGGGATTTTTGACCGATCAAATGGACGGTGTCGATGGAGGAGGAAGGCGGCGGCCCACTTTAAAGCAACGGCTAGGATTTAAAGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGCCGACTGTGAGCGTTAGAGACGGCGGCGGTGACGATGACGACCGGAGAGTACCGGATGTGGAGGTGATGAACACGCGCCGGTTTCCGGAGGAGAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTTCAGTCGCCGGGACCGACCGGGATGAACTTGGCCACGGCTCTGGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAACGGAGGGAGGTTTTGTGGAATCTAATAACAATGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTGGTGGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAAAAAAGAGAGAAGAGGCGGGAAATGATTCGATGTGCTGCGTGTGCATGGGGAGGAAAAAGGGTGCGGCATTTATCCCATGTGGGCACACGTTTTGTAGGGACCATGATGAGTACGATGCGGATGAGAACAGAGAATTGTTGGTGACGGGAAGGGGTAGTGCTTTTGCCTCAAGATTCGCAACAGACTGTTTTCATTGGACGGCACTGAACGATGGCCGTATCTCTGCGACATGTGGATGCGATGCGGGTGAGAAAACTTTTTCACGTCCGAGAAAAAAGTTTAATTAA

Coding sequence (CDS)

ATGAGTCTTCTCAGTGTGATTGTACAGGAAGCCGCCAGGCGAGAAAGGGAGGCTAGGACCATTTGGGGATTTTTGACCGATCAAATGGACGGTGTCGATGGAGGAGGAAGGCGGCGGCCCACTTTAAAGCAACGGCTAGGATTTAAAGTTATGGGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGCCGACTGTGAGCGTTAGAGACGGCGGCGGTGACGATGACGACCGGAGAGTACCGGATGTGGAGGTGATGAACACGCGCCGGTTTCCGGAGGAGAGGGAGTTGGATCGGAGTTGCTTGAGTCCGTTGTCCGTTCAGTCGCCGGGACCGACCGGGATGAACTTGGCCACGGCTCTGGCGGCGGAGCGTCGTTTAAGAGCGTCCCCACGTGGAACGGAGGGAGGTTTTGTGGAATCTAATAACAATGATTTTGACTCACTGGGCGGAATGATGGGAACGGGGACGCCGTTGAGGGTGTCGCTGATGCGGCTGCTGGAAGAAACAGACGGCGGTGGTGGTGGCGGTTGTGGGAATTTGGGAGTGGCGGAAAAAAAGAGAGAAGAGGCGGGAAATGATTCGATGTGCTGCGTGTGCATGGGGAGGAAAAAGGGTGCGGCATTTATCCCATGTGGGCACACGTTTTGTAGGGACCATGATGAGTACGATGCGGATGAGAACAGAGAATTGTTGGTGACGGGAAGGGGTAGTGCTTTTGCCTCAAGATTCGCAACAGACTGTTTTCATTGGACGGCACTGAACGATGGCCGTATCTCTGCGACATGTGGATGCGATGCGGGTGAGAAAACTTTTTCACGTCCGAGAAAAAAGTTTAATTAA

Protein sequence

MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGFRPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCRDHDEYDADENRELLVTGRGSAFASRFATDCFHWTALNDGRISATCGCDAGEKTFSRPRKKFN

Homology

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BLAST of Lsi01G004640 vs. ExPASy TrEMBL
Match: A0A5D3CM63 (Death-associated inhibitor of apoptosis 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006130 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.2e-117
Identity = 219/258 (84.88%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLLSVI+QEAARREREARTIWGFLTDQMDGVDGGGRRRP+LKQRLGFKVMGCCGATWGF
Sbjct: 1   MSLLSVILQEAARREREARTIWGFLTDQMDGVDGGGRRRPSLKQRLGFKVMGCCGATWGF 60

Query: 61  RPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATAL 120
           RPP+VSVRDGGG+DDDRRVPD+EVMNTRR  EERELDRSCLSPLSVQSPGP+GMNLATAL
Sbjct: 61  RPPSVSVRDGGGEDDDRRVPDLEVMNTRR--EERELDRSCLSPLSVQSPGPSGMNLATAL 120

Query: 121 AAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGNL 180
           AAERRLRASPRG EGG VE NNNDFDS  GMM TGTPLRVSL+RLLEET+GG  GG GNL
Sbjct: 121 AAERRLRASPRGAEGGIVEFNNNDFDSAVGMMETGTPLRVSLLRLLEETEGGCDGG-GNL 180

Query: 181 GVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCRDHDEYDADENRELLVTGRGSAFA 240
           GVAEKKREE GNDSMCCVCMGRKKGAAFIPCGHTFCR        + + L+V      FA
Sbjct: 181 GVAEKKREETGNDSMCCVCMGRKKGAAFIPCGHTFCR----RIVGDRKGLVV------FA 240

Query: 241 SRFATDCFHWTALNDGRI 259
           SRFATDCFHWTALNDGRI
Sbjct: 241 SRFATDCFHWTALNDGRI 245

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BLAST of Lsi01G004640 vs. ExPASy TrEMBL
Match: A0A0A0KMY7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G512860 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.1e-103
Identity = 192/218 (88.07%), Postives = 202/218 (92.66%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLLSVI+QEAARREREARTIWGFLTDQMDG+DGGGRRRP+LKQRLGFKVMGCCGATWGF
Sbjct: 1   MSLLSVILQEAARREREARTIWGFLTDQMDGIDGGGRRRPSLKQRLGFKVMGCCGATWGF 60

Query: 61  RPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATAL 120
           RPP+ SVRDGGG+DDDRRVPD+EVMNTRR  EEREL+RSCLSPLSVQSPGP+GMNLATAL
Sbjct: 61  RPPSDSVRDGGGEDDDRRVPDLEVMNTRR--EERELNRSCLSPLSVQSPGPSGMNLATAL 120

Query: 121 AAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGG-GGGGCGN 180
           AAERRLRASPRG EG  VE NNNDFDS  GMM TGTPLRVSL+RLLEETDGG   GG GN
Sbjct: 121 AAERRLRASPRGAEGDIVEFNNNDFDSAVGMMETGTPLRVSLLRLLEETDGGCDDGGGGN 180

Query: 181 LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           LGVAEKKR+E  NDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 LGVAEKKRDETRNDSMCCVCMGRKKGAAFIPCGHTFCR 216

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BLAST of Lsi01G004640 vs. ExPASy TrEMBL
Match: A0A6J1ISH8 (uncharacterized protein LOC111478017 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478017 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.7e-96
Identity = 182/218 (83.49%), Postives = 194/218 (88.99%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLL VI+QEAARRE EARTIWGFL+DQMDGVDGGGRRR +LKQRLGFKVMGCCGATWG 
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRRRQSLKQRLGFKVMGCCGATWGS 60

Query: 61  RPPTVSVRDGG-GDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATA 120
           RP T SVR GG  DDDD+RVP+ EV N RRF EER+LDRSC+SPLSV SP  +GMNLATA
Sbjct: 61  RPATGSVRGGGDDDDDDQRVPETEVTNPRRFQEERQLDRSCMSPLSVPSPATSGMNLATA 120

Query: 121 LAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGN 180
           LAAERRLRASPRG EGGFVES+NNDFDSLGGMM T TPL+VSL+RLL+ETDG  GG  G 
Sbjct: 121 LAAERRLRASPRGAEGGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGR-GI 180

Query: 181 LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 217

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BLAST of Lsi01G004640 vs. ExPASy TrEMBL
Match: A0A6J1GH19 (uncharacterized protein LOC111453829 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453829 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.1e-95
Identity = 181/219 (82.65%), Postives = 195/219 (89.04%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLL VI+QEAARRE EARTIWGFL+DQMDGVDGGGRRR +LKQRLGFKVMGCCGATWG 
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRRRQSLKQRLGFKVMGCCGATWGS 60

Query: 61  RPPTVSVRDGGG--DDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLAT 120
           RP T SVR GGG  DDDD+RVP+ EVMN RRF +ER+LDRS +SPLSV SP  +GMNLAT
Sbjct: 61  RPATGSVRGGGGDDDDDDQRVPETEVMNPRRFQDERQLDRSFMSPLSVPSPATSGMNLAT 120

Query: 121 ALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCG 180
           ALAAERRLRASPRG E GFVES+NNDFDSLGGMM T TPL+VSL+RLL+ETDG  GG  G
Sbjct: 121 ALAAERRLRASPRGAEVGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGR-G 180

Query: 181 NLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           +LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 SLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218

[Close]

BLAST of Lsi01G004640 vs. ExPASy TrEMBL
Match: A0A6J1BTZ3 (E3 ubiquitin-protein ligase RFWD2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005336 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 4.9e-93
Identity = 181/223 (81.17%), Postives = 191/223 (85.65%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQM-DGVDGGGRRRPTLKQRLGFKVMGCCG--AT 60
           MSLLSVI+QEAARREREARTIWGFL++QM DGVDGGGRRR TLKQRLGFKVMGCCG  AT
Sbjct: 1   MSLLSVILQEAARREREARTIWGFLSEQMDDGVDGGGRRRRTLKQRLGFKVMGCCGGVAT 60

Query: 61  WGFRPPTVSVRDGGGDD---DDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGM 120
           WG RPP VSVR G  DD   DDRRVPD EVMNTR  PEER+LDRSCLSP S  SP P+GM
Sbjct: 61  WGCRPPAVSVRGGDDDDDDEDDRRVPDAEVMNTRPSPEERDLDRSCLSPFSGSSPAPSGM 120

Query: 121 NLATALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGG 180
           NLATALAAERRLRASPRG EGG VE NN++FDSL G+  TGTPLRVSL+RLLEETDGGG 
Sbjct: 121 NLATALAAERRLRASPRGAEGGLVEPNNDEFDSLAGIAVTGTPLRVSLLRLLEETDGGG- 180

Query: 181 GGCGNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
                 GVAEKKREEAGNDS+CCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 ------GVAEKKREEAGNDSVCCVCMGRKKGAAFIPCGHTFCR 216

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BLAST of Lsi01G004640 vs. NCBI nr
Match: TYK13013.1 (death-associated inhibitor of apoptosis 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 431.8 bits (1109), Expect = 4.5e-117
Identity = 219/258 (84.88%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLLSVI+QEAARREREARTIWGFLTDQMDGVDGGGRRRP+LKQRLGFKVMGCCGATWGF
Sbjct: 1   MSLLSVILQEAARREREARTIWGFLTDQMDGVDGGGRRRPSLKQRLGFKVMGCCGATWGF 60

Query: 61  RPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATAL 120
           RPP+VSVRDGGG+DDDRRVPD+EVMNTRR  EERELDRSCLSPLSVQSPGP+GMNLATAL
Sbjct: 61  RPPSVSVRDGGGEDDDRRVPDLEVMNTRR--EERELDRSCLSPLSVQSPGPSGMNLATAL 120

Query: 121 AAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGNL 180
           AAERRLRASPRG EGG VE NNNDFDS  GMM TGTPLRVSL+RLLEET+GG  GG GNL
Sbjct: 121 AAERRLRASPRGAEGGIVEFNNNDFDSAVGMMETGTPLRVSLLRLLEETEGGCDGG-GNL 180

Query: 181 GVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCRDHDEYDADENRELLVTGRGSAFA 240
           GVAEKKREE GNDSMCCVCMGRKKGAAFIPCGHTFCR        + + L+V      FA
Sbjct: 181 GVAEKKREETGNDSMCCVCMGRKKGAAFIPCGHTFCR----RIVGDRKGLVV------FA 240

Query: 241 SRFATDCFHWTALNDGRI 259
           SRFATDCFHWTALNDGRI
Sbjct: 241 SRFATDCFHWTALNDGRI 245

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BLAST of Lsi01G004640 vs. NCBI nr
Match: XP_038883240.1 (uncharacterized protein LOC120074251 isoform X1 [Benincasa hispida])

HSP 1 Score: 405.2 bits (1040), Expect = 4.5e-109
Identity = 201/217 (92.63%), Postives = 206/217 (94.93%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLLSVI+QEAARREREARTIWGFLTDQMDGVDGGGR+RP+LKQRLGFKVMGCCGATWGF
Sbjct: 1   MSLLSVILQEAARREREARTIWGFLTDQMDGVDGGGRQRPSLKQRLGFKVMGCCGATWGF 60

Query: 61  RPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATAL 120
           R PTVSVR+GGGDDDDRRVPDVEV NTRR  EERELDRSCLSPLSVQSPGP+GMNLATAL
Sbjct: 61  RSPTVSVRNGGGDDDDRRVPDVEVTNTRR--EERELDRSCLSPLSVQSPGPSGMNLATAL 120

Query: 121 AAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGNL 180
           AAERRLRASPRG EGG VE NNNDFDSLGGMMGTGTPLRVSLMRLLEETD GG GGCGNL
Sbjct: 121 AAERRLRASPRGMEGGIVEFNNNDFDSLGGMMGTGTPLRVSLMRLLEETD-GGRGGCGNL 180

Query: 181 GVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           GVAE KREE GNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 GVAETKREEPGNDSMCCVCMGRKKGAAFIPCGHTFCR 214

[Close]

BLAST of Lsi01G004640 vs. NCBI nr
Match: XP_022977839.1 (uncharacterized protein LOC111478017 isoform X1 [Cucurbita maxima])

HSP 1 Score: 360.9 bits (925), Expect = 9.8e-96
Identity = 182/218 (83.49%), Postives = 194/218 (88.99%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLL VI+QEAARRE EARTIWGFL+DQMDGVDGGGRRR +LKQRLGFKVMGCCGATWG 
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRRRQSLKQRLGFKVMGCCGATWGS 60

Query: 61  RPPTVSVRDGG-GDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATA 120
           RP T SVR GG  DDDD+RVP+ EV N RRF EER+LDRSC+SPLSV SP  +GMNLATA
Sbjct: 61  RPATGSVRGGGDDDDDDQRVPETEVTNPRRFQEERQLDRSCMSPLSVPSPATSGMNLATA 120

Query: 121 LAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGN 180
           LAAERRLRASPRG EGGFVES+NNDFDSLGGMM T TPL+VSL+RLL+ETDG  GG  G 
Sbjct: 121 LAAERRLRASPRGAEGGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGR-GI 180

Query: 181 LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 217

[Close]

BLAST of Lsi01G004640 vs. NCBI nr
Match: XP_022950854.1 (uncharacterized protein LOC111453829 isoform X1 [Cucurbita moschata])

HSP 1 Score: 358.2 bits (918), Expect = 6.3e-95
Identity = 181/219 (82.65%), Postives = 195/219 (89.04%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLL VI+QEAARRE EARTIWGFL+DQMDGVDGGGRRR +LKQRLGFKVMGCCGATWG 
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRRRQSLKQRLGFKVMGCCGATWGS 60

Query: 61  RPPTVSVRDGGG--DDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLAT 120
           RP T SVR GGG  DDDD+RVP+ EVMN RRF +ER+LDRS +SPLSV SP  +GMNLAT
Sbjct: 61  RPATGSVRGGGGDDDDDDQRVPETEVMNPRRFQDERQLDRSFMSPLSVPSPATSGMNLAT 120

Query: 121 ALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCG 180
           ALAAERRLRASPRG E GFVES+NNDFDSLGGMM T TPL+VSL+RLL+ETDG  GG  G
Sbjct: 121 ALAAERRLRASPRGAEVGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGR-G 180

Query: 181 NLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           +LGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 SLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218

[Close]

BLAST of Lsi01G004640 vs. NCBI nr
Match: KAG7033976.1 (putative E3 ubiquitin-protein ligase LUL4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 355.5 bits (911), Expect = 4.1e-94
Identity = 180/220 (81.82%), Postives = 194/220 (88.18%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MSLL VI+QEAARRE EARTIWGFL+DQMDGVDGGGRRR +LKQRLGFKVMGCCGATWG 
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRRRQSLKQRLGFKVMGCCGATWGS 60

Query: 61  RPPTVSVRDGGG---DDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLA 120
           RP T SVR GGG   DDDD+RVP+ EVMN RRF +ER+LDRS +SPLSV SP  +GMNLA
Sbjct: 61  RPATGSVRGGGGDDDDDDDQRVPETEVMNPRRFQDERQLDRSFMSPLSVPSPATSGMNLA 120

Query: 121 TALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGC 180
           TALAAERRLRASPRG E GFVES+NNDFDSLGGMM T TPL+VSL+RLL+ETDG  GG  
Sbjct: 121 TALAAERRLRASPRGAEVGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGR- 180

Query: 181 GNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
           G+ GVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 GSSGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 219

[Close]

BLAST of Lsi01G004640 vs. TAIR 10
Match: AT4G03965.1 (RING/U-box superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 4.8e-32
Identity = 94/228 (41.23%), Postives = 108/228 (47.37%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGF 60
           MS L V   +A     ++ T    L  QMDG+D   RR+ TLK+RLGFK +GCCG TWG 
Sbjct: 1   MSQLGVETSQAQPETPQSTTFLDLLRLQMDGLDRSRRRKRTLKERLGFKRIGCCGPTWGL 60

Query: 61  RPPTVSVRDGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATAL 120
           R     +    G+DDD              P E   +R       V  P   GMNLATAL
Sbjct: 61  R-----LTSNTGEDDDE-------------PFE---NRLVSGSDHVTGPLNQGMNLATAL 120

Query: 121 AAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGGGGGGCGNL 180
           AAER  R       G                    TPL+VSLMRLLEET          +
Sbjct: 121 AAERNYRTEETEASGSL------------------TPLKVSLMRLLEET-------AERV 180

Query: 181 GVAEKKREE-----------AGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
            V E + E              NDS+CCVCMGRKKGAAFIPCGHTFCR
Sbjct: 181 VVEENETERVTASASSTVRGVNNDSVCCVCMGRKKGAAFIPCGHTFCR 182

[Close]

BLAST of Lsi01G004640 vs. TAIR 10
Match: AT4G22250.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 5.6e-25
Identity = 87/215 (40.47%), Postives = 94/215 (43.72%), Query Frame = 0


Query: 9   QEAARREREARTIWGFLTDQMDGVDGGGRRRPTLKQRLGFKVMGCCGATWGFRPPTVSVR 68
           Q      R   T    L  QM G D   RR+ TLK+RL FK +GCCG TW  R    +  
Sbjct: 11  QRMTETHRTTTTFLDLLRRQMSGHD-LTRRKRTLKERLRFKCIGCCGPTWNLRLTNNTTL 70

Query: 69  DGGGDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPTGMNLATALAAERRLRA 128
                 DD               E  EL       L       TGMNLATAL AER  R 
Sbjct: 71  HSPTSRDD---------------EIEELQIETGLVLESDHGSGTGMNLATALEAERYNRG 130

Query: 129 SPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEET------DGGGGGGCGNLGV 188
                     ES   + D         TP RVSLMRLLEET      D G         +
Sbjct: 131 ----------ESTEAEADM--------TPRRVSLMRLLEETAEKIVDDDGKETEILTASI 187

Query: 189 AEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
                   GNDS+CCVCMGRKKGAAFIPCGHTFCR
Sbjct: 191 GTL----TGNDSVCCVCMGRKKGAAFIPCGHTFCR 187

[Close]

BLAST of Lsi01G004640 vs. TAIR 10
Match: AT1G62370.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-20
Identity = 78/225 (34.67%), Postives = 104/225 (46.22%), Query Frame = 0


Query: 1   MSLLSVIVQEAARREREARTIWGFLTDQMDGVDGGGR---RRPTLKQRLGFKVMGCCGAT 60
           MS L  +++E+    R  RT+   L ++ D ++GG R   +  TLKQRL F  +GCCG  
Sbjct: 1   MSQLGDLLRESEDGTRSERTMMMSLLEE-DQINGGDRTMSKWTTLKQRLRFDWVGCCG-- 60

Query: 61  WGFRPPTVSVRDGG-----GDDDDRRVPDVEVMNTRRFPEERELDRSCLSPLSVQSPGPT 120
              +P T+ +R         DDDD      +V++        ELD  CL+  S +     
Sbjct: 61  ---KPLTLRLRQAETPIVVDDDDDEEESQNQVVDFSDPGTGTELD--CLTRGSTR----- 120

Query: 121 GMNLATALAAERRLRASPRGTEGGFVESNNNDFDSLGGMMGTGTPLRVSLMRLLEETDGG 180
             NLA ALA ER    +   T                         +V LMRLL E+DG 
Sbjct: 121 --NLAEALAEERLAHVTEEET----------------------AVTKVPLMRLLAESDG- 177

Query: 181 GGGGCGNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCR 218
                      +      GND +CCVCMGR+KGAAFIPCGHT+CR
Sbjct: 181 ----------CDSTTTWLGNDPLCCVCMGREKGAAFIPCGHTYCR 177

[Close]

BLAST of Lsi01G004640 vs. TAIR 10
Match: AT3G25030.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 31/61 (50.82%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 157 PLRVSLMRLLEETDGGGGGGCGNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFC 216
           P R+SLM LLEE +G       +    E+       +  CCVCM R KGAAFIPCGHTFC
Sbjct: 163 PARMSLMELLEENEGQMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFC 222

Query: 217 R 218
           R
Sbjct: 223 R 223

[Close]

BLAST of Lsi01G004640 vs. TAIR 10
Match: AT3G25030.2 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 31/61 (50.82%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 157 PLRVSLMRLLEETDGGGGGGCGNLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFC 216
           P R+SLM LLEE +G       +    E+       +  CCVCM R KGAAFIPCGHTFC
Sbjct: 163 PARMSLMELLEENEGQMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFC 222

Query: 217 R 218
           R
Sbjct: 223 R 223

The following BLAST results are available for this feature:

BLAST of Lsi01G004640 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi01G004640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CM63	2.2e-117	84.88	Death-associated inhibitor of apoptosis 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KMY7	1.1e-103	88.07	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G512860 P...	[more]
A0A6J1ISH8	4.7e-96	83.49	uncharacterized protein LOC111478017 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GH19	3.1e-95	82.65	uncharacterized protein LOC111453829 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1BTZ3	4.9e-93	81.17	E3 ubiquitin-protein ligase RFWD2 isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Lsi01G004640 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK13013.1	4.5e-117	84.88	death-associated inhibitor of apoptosis 1-like [Cucumis melo var. makuwa]	[more]
XP_038883240.1	4.5e-109	92.63	uncharacterized protein LOC120074251 isoform X1 [Benincasa hispida]	[more]
XP_022977839.1	9.8e-96	83.49	uncharacterized protein LOC111478017 isoform X1 [Cucurbita maxima]	[more]
XP_022950854.1	6.3e-95	82.65	uncharacterized protein LOC111453829 isoform X1 [Cucurbita moschata]	[more]
KAG7033976.1	4.1e-94	81.82	putative E3 ubiquitin-protein ligase LUL4, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Lsi01G004640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03965.1	4.8e-32	41.23	RING/U-box superfamily protein	[more]
AT4G22250.1	5.6e-25	40.47	RING/U-box superfamily protein	[more]
AT1G62370.1	3.2e-20	34.67	RING/U-box superfamily protein	[more]
AT3G25030.1	1.9e-09	50.82	RING/U-box superfamily protein	[more]
AT3G25030.2	1.9e-09	50.82	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 180..221 e-value: 6.5E-7 score: 30.7
None	No IPR available	PANTHER	PTHR46629	OS01G0917900 PROTEIN	coord: 17..217
None	No IPR available	PANTHER	PTHR46629:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 17..217
None	No IPR available	CDD	cd16449	RING-HC	coord: 196..218 e-value: 8.54436E-5 score: 37.0617
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 187..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G004640.1	Lsi01G004640.1	mRNA