Lsi01G004450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G004450
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mitogen-activated protein kinase kinase kinase 2-like
Location	chr01: 3704520 .. 3706988 (-)
RNA-Seq Expression	Lsi01G004450
Synteny	Lsi01G004450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGATAAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCGTTGCTACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGTCGGTTGAGTTCACTCACTCGGATTTTCTCAAGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTTCAAATGTTATAGGTTATAAGGGGTTTGAAGTTACGTTGGAAAATGGGAATTTGATGTGTAATATTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGTGATGGAGGAGGCGGGTGGCCGATTAGATGAAGCGACGGCCCAATTGTATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGACATTAAGTGTTGTAATCTTTTGGTTGGTGAAGATGGGATTAAGATTGCGGATTTTGGTTGCGCGCGGCGGGTGGAGGAAGTTTCCGGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAAGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCTGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCAAGGTTCATGTCGGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAAACCCAGAAGAGCGGTGGTCTGTGGATGAGCTTTTGAAACACCCTTTTGTTAACGAGCAGAAATCTCAACCCAAACAGAATTCAAGAACACCAACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGAAACTAAGCGAAGTGTCCACAATTCAGTCTTCTGGAATACCCAATTGGGAGTGCGATGAAGATTGGGCAACAGTGAGAAGCATTGGCTTGGAAGAGGAAGACAGAGAAGAGAACGATTTCGTTTCTGTATTGGAAATGCCCTGTTCTTCTCAATCCATCGAAATGGCAACTTACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTGTAGCAGAAAAAGCAGCAAGGCACAAAACAGAGACTTGCCTGTTATGTCCTCTGTTTGCAACCAAATTGTATTATGGAAGACTATTAGTTTTTCTAATAATTATAAATTATGTATTATCATTTCTTTGCTTCTTGTCTTTCCATTTAGGGGTTTATTTTATCTTACATGTTTGAGATATAATGAAGATATTTTGATTCATTACGAAAATATGAACATGTCGATCCATCTCTGTTTACTTGAGTTGAGTATCTTGTTTGAAGGCTGAAAGCTTGCCTTAGAAAATTAATGGTACGATGTGAAGTTAATTAATTTGATGAAGAAAATTTAGTGGTGCATGATTAGCTAATGGTTGAGAAATTAAATTGTGATGCCATTAATGATGCCTGCCTATGCCTAGCTTGAATTTTTTAGTTGATTGAAGTATAGATCTTACCCACTGGAAAAGAATAATTCAGAAGTTGTTTTGAAAATGTAACTTAGCAGCAAAGCCAAGCCGACAATAATGTTGGTGTAGTTTTTTTCACAGTTAAAAGCAGTCAAAGCCGACAATAATGATGCTCAGGCTCAGCACAAATCGTCCATATTCTGGATCTGGATTTTCCTTTGGTTTCTCCTGCTTTTCTCTGGCTCCACAATGTATATTAAAGGTTTTTCCATCAATATGATTCTGTTTTGAATTCATCATACGAGTATGTTATAGTATAGTTAGTTTTTATGTTTAAATTTCTATTCTAATGCTTTCAAGGGTTGGAACCAAATGATATGTCCTAAGAATGAATTTTGCAGAGGCCAAATTCTTCATGGTTAAAGCCGAAATATATATAAAGAGAAAAAACAAGTAGCTACAGAAGAATGACAGAAGCGAAGGTGTTCTCTATTACAAACCTGAATTTATCACAATTTTTTTTTCAGTAGAATCCATTCTTGTTTCACTGCTTTACCTTCCTACTTTAATCAGACACAAGAAGCCAAAACCTTATCTTCTCTTGCCATTGTGCATATATAAGAAGTAAACAGCCATCATCAACCCATCATGGGGTACCCAGATCTTAAATAATATGAGGTGAAATCGAAGCGCAAAACTTGAAGAAGTGAGATTCGCTAAGCTACTAAGCTATATGGGGTCCTTAAGAATTCTTCTTGCTTCCCGATCGAGACAATCTGCGATCCACGGATTGTAATTGGCCCCGAAGGTTTTGGTTTTCCTCAAGTAAACGGTCATACTCGAGCAGTAATCCTTCAGATTGTTTCCTTAAAGCTACTACATTGGCTTCTGAAGTTGACACATCTTTTGTTTTCGAATCAAGTTCCAATTCCAACTGTTTGA

mRNA sequence

ATGGAGTGGATAAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCGTTGCTACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGTCGGTTGAGTTCACTCACTCGGATTTTCTCAAGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTTCAAATGTTATAGGTTATAAGGGGTTTGAAGTTACGTTGGAAAATGGGAATTTGATGTGTAATATTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGTGATGGAGGAGGCGGGTGGCCGATTAGATGAAGCGACGGCCCAATTGTATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGACATTAAGTGTTGTAATCTTTTGGTTGGTGAAGATGGGATTAAGATTGCGGATTTTGGTTGCGCGCGGCGGGTGGAGGAAGTTTCCGGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAAGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCTGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCAAGGTTCATGTCGGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAAACCCAGAAGAGCGGTGGTCTGTGGATGAGCTTTTGAAACACCCTTTTGTTAACGAGCAGAAATCTCAACCCAAACAGAATTCAAGAACACCAACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGAAACTAAGCGAAGTGTCCACAATTCAGTCTTCTGGAATACCCAATTGGGAGTGCGATGAAGATTGGGCAACAGTGAGAAGCATTGGCTTGGAAGAGGAAGACAGAGAAGAGAACGATTTCGTTTCTGTATTGGAAATGCCCTGTTCTTCTCAATCCATCGAAATGGCAACTTACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTGTAGCAGAAAAAGCAGCAAGGCACAAAACAGAGACTTGCCTGTTATGTCCTCTGTTTGCAACCAAATTAATTCTTCTTGCTTCCCGATCGAGACAATCTGCGATCCACGGATTGTAATTGGCCCCGAAGGTTTTGGTTTTCCTCAAGTAAACGGTCATACTCGAGCAGTAATCCTTCAGATTGTTTCCTTAAAGCTACTACATTGGCTTCTGAAGTTGACACATCTTTTGTTTTCGAATCAAGTTCCAATTCCAACTGTTTGA

Coding sequence (CDS)

ATGGAGTGGATAAGAGGCCGGACCATCGGCCGTGGCTCCTCCGCTGCGGTCTCCGTTGCTACCGATCTTCGGTCTGGTCAAAGGCTGGCGGTTAAGTCGGTTGAGTTCACTCACTCGGATTTTCTCAAGAGGGAACAGAGGATTCTTTCTCAATTGAATTGTTCAAATGTTATAGGTTATAAGGGGTTTGAAGTTACGTTGGAAAATGGGAATTTGATGTGTAATATTTTGATGGAGTTTGCGCCTGGTGGGTCGATTTTGGATGTGATGGAGGAGGCGGGTGGCCGATTAGATGAAGCGACGGCCCAATTGTATACTCGAGAGATTTTAAGTGGGCTCGAGTATGTTCATTCTAATGGTGTGGTACATTGTGACATTAAGTGTTGTAATCTTTTGGTTGGTGAAGATGGGATTAAGATTGCGGATTTTGGTTGCGCGCGGCGGGTGGAGGAAGTTTCCGGTGGAAATTTGGCTGGAACGCCGATATTCATGGCGCCAGAAGTGGCGCGTGGAGAAAAACAGGGGTTTGCAGCTGATGTTTGGGGAGTGGGTTGCGCAGTGATCCAGATGGTGACAGGCCGAGCTCCGTGGGCGAATGTGTCGGATCCGGTGGCTGCCATTTACAGAATTGGGTCCGGCGATGATTTGCCGGAGATTCCAAGGTTCATGTCGGAACAGGGCAAGGACTTTTTGCGGCGGTGTTTGATCAGAAACCCAGAAGAGCGGTGGTCTGTGGATGAGCTTTTGAAACACCCTTTTGTTAACGAGCAGAAATCTCAACCCAAACAGAATTCAAGAACACCAACGAGTATTTTGGAACAAGCATTATGGGACACGATGAACGATTCGGAAACGGTTCAAAGTCCGATCAGACCAAAAATTCACAGAACGCCATTGCAGAGGATACAGAAACTAAGCGAAGTGTCCACAATTCAGTCTTCTGGAATACCCAATTGGGAGTGCGATGAAGATTGGGCAACAGTGAGAAGCATTGGCTTGGAAGAGGAAGACAGAGAAGAGAACGATTTCGTTTCTGTATTGGAAATGCCCTGTTCTTCTCAATCCATCGAAATGGCAACTTACAATGGAGTTGGTGATCAGGATTATATTAATGTTAGTGTTAGTAGAAGTAGTGATTATTGTAGCAGAAAAAGCAGCAAGGCACAAAACAGAGACTTGCCTGTTATGTCCTCTGTTTGCAACCAAATTAATTCTTCTTGCTTCCCGATCGAGACAATCTGCGATCCACGGATTGTAATTGGCCCCGAAGGTTTTGGTTTTCCTCAAGTAAACGGTCATACTCGAGCAGTAATCCTTCAGATTGTTTCCTTAAAGCTACTACATTGGCTTCTGAAGTTGACACATCTTTTGTTTTCGAATCAAGTTCCAATTCCAACTGTTTGA

Protein sequence

MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGYKGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNGVVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPEERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMATYNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQINSSCFPIETICDPRIVIGPEGFGFPQVNGHTRAVILQIVSLKLLHWLLKLTHLLFSNQVPIPTV

Homology

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BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.7e-77
Identity = 161/341 (47.21%), Postives = 218/341 (63.93%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           M W RG+T+GRGS+A VS AT   SG+ LAVKS EF  S+FL+RE +ILS LN   VIGY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFEVTLE----NGNLMC-NILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEY 120
           +G E+T E    NG     ++LME+AP G++ DV  + GG +DEA    YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFGCA+ VE      + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWGVGCAVIQMVTGRAPW--ANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFL 240
           QG  +D+W VGC VI+MVTG  PW  A+ +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRNPEERWSVDELLKHPFVNEQKSQPKQN--SRTPTSILEQALWDTMNDSETVQSP 300
            +CL +   ERW+  +LL HPF+  ++ +      + +PTS+ +Q  W ++ +  +    
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSEDRS 300

Query: 301 IRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECD-EDWATVR 330
              + H    +RI  LS +  +       W+ D EDW TVR
Sbjct: 301 SWWECHED--ERIGVLSWIGHVVVES--TWDLDGEDWITVR 337

[Close]

BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 6.1e-77
Identity = 156/350 (44.57%), Postives = 213/350 (60.86%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEW RGR +GRGS+A V  A    S + LAVKS E   S+FL+RE +ILS L+   VIGY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFEVTLE-NGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSN 120
           +G E   E NG +M N+LME+AP G++ D   + GGR+DE     YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNLLVGEDG-IKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFGCA+RV+ V    + GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWGVGCAVIQMVTGRAPWANV---SDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCL 240
           D+W VGC +I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRNPEERWSVDELLKHPFVN-----EQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPI 300
            R   ERW+  +LL HPF+      E    P   S +PTS+ +Q  W ++ + E  ++  
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 RPKIHRTPLQRI-------QKLSEVSTIQSSGIPNWECD---EDWATVRS 331
             K  R  L R+       +++  +  +   G+    CD    DW  VR+
Sbjct: 301 IQKDSR-DLDRLSLWGCYSERIGRLKCV--GGLDGTRCDMEGGDWIMVRA 347

[Close]

BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.7e-56
Identity = 128/357 (35.85%), Postives = 196/357 (54.90%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSG----QRLAVKSVEFTHSDFLKREQRILSQL-NCS 60
           MEW+RG TIG G+ + VS AT  R+       +AVKS +   +  L  E+ +L  L +C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVIGYKGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGR-LDEATAQLYTREILSGLE 120
            +I   G + T+ENG  M N+L+E+A  GS+   M++ GG  L E+T + +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEE-----VSGGNLAGTPIFMAPEV 180
           ++H+ G  HCDIK  N+L+  DG +KIADFG A RV+           + GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWGVGCAVIQMVTGRAPWA--NVSDPVAAIYRIGSGDDLPEIPRFMSEQ 240
               + G AADVW +GCAV++M +G+  W+    S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRNPEERWSVDELLKHPFVN-EQKSQPKQN-----------SRTPTSILEQ 300
           GKDFL +C +++P +RW+ + LL H FV  + +   ++N             +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 ALWD--TMNDSETVQSPIRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECDEDWATVR 330
             WD  T++ + +  SP+                 + ++ S  IP+W     W TVR
Sbjct: 301 DDWDSFTLDSNPSFDSPV---------------ERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-49
Identity = 116/291 (39.86%), Postives = 171/291 (58.76%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFT-----------HSDFLKREQRIL 60
           + W +G+ IGRG+   V +  +L SG+ LAVK V  T           H   L+ E ++L
Sbjct: 66  IRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLL 125

Query: 61  SQLNCSNVIGYKGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREI 120
             L+  N++ Y G   T+     + NIL+EF PGGSI  ++E+ G    E+  + YT ++
Sbjct: 126 KNLSHPNIVRYLG---TVREDETL-NILLEFVPGGSISSLLEKFGA-FPESVVRTYTNQL 185

Query: 121 LSGLEYVHSNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEE---VSGG-NLAGTPIFM 180
           L GLEY+H++ ++H DIK  N+LV   G IK+ADFG +++V E   +SG  ++ GTP +M
Sbjct: 186 LLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWM 245

Query: 181 APEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMS 240
           APEV       F+AD+W VGC VI+MVTG+APW+     +AAI+ IG+    P IP  +S
Sbjct: 246 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNIS 305

Query: 241 EQGKDFLRRCLIRNPEERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQA 276
               DFL +CL + P  R +  ELLKHPFV  +  Q +  S+  TS ++ +
Sbjct: 306 SDANDFLLKCLQQEPNLRPTASELLKHPFVTGK--QKESASKDLTSFMDNS 349

[Close]

BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 2.4e-49
Identity = 112/270 (41.48%), Postives = 161/270 (59.63%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEF-----------THSDFLKREQRIL 60
           + W +G+ IGRG+   V +  +L SG+ LAVK V              H   L+ E ++L
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 61  SQLNCSNVIGYKGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREI 120
             L+  N++ Y G   T+   + + NIL+EF PGGSI  ++E+  G   E+  + YTR++
Sbjct: 127 KNLSHPNIVRYLG---TVREDDTL-NILLEFVPGGSISSLLEKF-GPFPESVVRTYTRQL 186

Query: 121 LSGLEYVHSNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEEVS----GGNLAGTPIFM 180
           L GLEY+H++ ++H DIK  N+LV   G IK+ADFG +++V E++      ++ GTP +M
Sbjct: 187 LLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWM 246

Query: 181 APEVARGEKQGFAADVWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMS 240
           APEV       F+AD+W VGC VI+MVTG+APW+     VAAI+ IG+    P IP  +S
Sbjct: 247 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLS 306

Query: 241 EQGKDFLRRCLIRNPEERWSVDELLKHPFV 255
              KDFL +CL   P  R +  ELLKHPFV
Sbjct: 307 SDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

[Close]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL
Match: A0A5D3CNL0 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006310 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.4e-198
Identity = 348/403 (86.35%), Postives = 370/403 (91.81%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRT+GRGSSAAVSVATDLRSGQ +AVK+VEF+  DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCARRVE+V GGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSILEQ +WDT+ND ETV+SPIRPKIHRTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVSTI     PNWECDEDW TVRSIGL     EEND VSV+E PCSSQS+EMA+
Sbjct: 301 RIQQLNEVSTIG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            N VGDQDYINVS SRSSDYCSRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

[Close]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL
Match: A0A1S3B3E3 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485329 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.5e-198
Identity = 347/403 (86.10%), Postives = 369/403 (91.56%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRT+GRGSSAAVSVATDLRSGQ +AVK+VEF+  DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCARRVE+V GGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSILEQ +WDT+ND ETV+SPIRPKIHRTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVST      PNWECDEDW TVRSIGL     EEND VSV+E PCSSQS+EMA+
Sbjct: 301 RIQQLNEVST----NGPNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            N VGDQDYINVS SRSSDYCSRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

[Close]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL
Match: A0A0A0KL63 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513560 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.7e-197
Identity = 346/403 (85.86%), Postives = 369/403 (91.56%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRTIGRGSSAAVSVATD+R GQ +AVKSVEF+H DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTIGRGSSAAVSVATDIRFGQVMAVKSVEFSHLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTRE+LSGL+YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEKAGGRLDEATAQFYTREVLSGLQYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+L+GEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILMGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGR PWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRVPWANLSDPLAAIYRIGSGDDLPEIPRIMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSIL+Q +WDT+ND ETV+SPIRPKI RTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILDQGIWDTVNDPETVESPIRPKIQRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVSTI   GIPNWECDEDW TVRSIGL     EEND VSV+E P S QSI+M T
Sbjct: 301 RIQQLNEVSTI---GIPNWECDEDWITVRSIGL-----EENDIVSVMETPSSFQSIKMET 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            NGVGDQDYINVS SRSSDY SRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNGVGDQDYINVSRSRSSDYGSRKSSKAKNRDFPAMSSVFNQI 395

[Close]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL
Match: A0A6J1GDW5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111453267 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 6.3e-186
Identity = 330/405 (81.48%), Postives = 360/405 (88.89%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEW RG TIGRGSSAAVSVATDL SGQ LAVKSVEFT SD LKREQRILS+ NC  VI Y
Sbjct: 20  MEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDCLKREQRILSRFNCPRVIAY 79

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENG LMCN+LMEFAPGGSILDVME AGGRLDEA AQ YTREILSGLEYVH+NG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEARAQFYTREILSGLEYVHTNG 139

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCAR+VE+VS  NLAGT IFMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEDVSCRNLAGTLIFMAPEVARGEKQGFAADV 199

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+PRF+S+QGKDFLRRCLIR+PE
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFISDQGKDFLRRCLIRDPE 259

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV ELLKHPFV+EQKS PKQNSRTPTSILEQ  W TMN+SETVQ PI PK++RTPLQ
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSETVQIPIMPKLYRTPLQ 319

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+LSE+S IQSS IPNW+CD+DW TV+SI +EEED E+++FVSVLE+PC SQSI+MAT
Sbjct: 320 RIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQSEFVSVLELPCFSQSIKMAT 379

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQINS 406
            NGVGDQ YINVS+       SR+SSKA+ RDLP MSSV NQI S
Sbjct: 380 KNGVGDQTYINVSI-------SRESSKAKKRDLPAMSSVSNQIVS 417

[Close]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL
Match: A0A6J1EMC5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111433865 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 6.8e-180
Identity = 327/407 (80.34%), Postives = 353/407 (86.73%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEW RGR IG GSSAAVSVATDLRSGQ LAVKSVEF  SDF+KREQRILSQLN S VI  
Sbjct: 13  MEWTRGRVIGCGSSAAVSVATDLRSGQLLAVKSVEFDRSDFIKREQRILSQLNSSRVIAC 72

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFA GGSILD ME AGGRLDEATAQ YTREILSGLEYVHSNG
Sbjct: 73  KGFDVTLENGNLMCNLLMEFASGGSILDAMEAAGGRLDEATAQFYTREILSGLEYVHSNG 132

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKC N+LVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 133 VVHCDIKCSNILVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 192

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGC VIQMVTGRAPW ++SDPVAAIYRIGS DDLPEIPRFMSEQGK+FLRRCLIR+PE
Sbjct: 193 WAVGCTVIQMVTGRAPWEDMSDPVAAIYRIGSSDDLPEIPRFMSEQGKEFLRRCLIRDPE 252

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV ELLKHPFV+EQKS+PKQN RTPTSILEQ LW+TMND ETVQSPIRPK +RTPLQ
Sbjct: 253 ERWSVSELLKHPFVHEQKSRPKQNPRTPTSILEQELWNTMNDLETVQSPIRPKFYRTPLQ 312

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGL--EEEDREENDFVSVLEMPCSSQSIEM 360
           RIQ+LSEVS  QS GIPNWE +EDW TVRS  +  EEEDRE+ DF+S LE+PCSS+S+  
Sbjct: 313 RIQQLSEVSKFQSFGIPNWESNEDWVTVRSKDMEEEEEDREKRDFISELELPCSSESMAT 372

Query: 361 ATYNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQINS 406
            T NG GDQ+YI  S+S+SSDYC +K       + P MSSV +QI S
Sbjct: 373 TTQNGAGDQNYI--SISKSSDYCCKKI------NFP-MSSVFSQIVS 410

[Close]

BLAST of Lsi01G004450 vs. NCBI nr
Match: XP_038883085.1 (mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida])

HSP 1 Score: 725.7 bits (1872), Expect = 2.5e-205
Identity = 367/442 (83.03%), Postives = 383/442 (86.65%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEW+RGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTH DFLKREQRILSQLNCSNVIGY
Sbjct: 1   MEWMRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHLDFLKREQRILSQLNCSNVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLE+GNLMCN+LMEFA GGSILD MEEAGGRLDEATAQLYTREILSGLEYVHSNG
Sbjct: 61  KGFDVTLEDGNLMCNLLMEFASGGSILDAMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCARRVEEVSGGN AGTPIFMAPEVARG+KQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEEVSGGNWAGTPIFMAPEVARGKKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           WGVGCAVIQMVTGR PWANVSDPVAAIYRIG+GD+LPEIPRFMSEQGKDFLRRCLIR+PE
Sbjct: 181 WGVGCAVIQMVTGRPPWANVSDPVAAIYRIGAGDELPEIPRFMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTP-------------------------------- 300
           +RWSV+ELLKHPFV EQKS PKQNSRTP                                
Sbjct: 241 KRWSVNELLKHPFVYEQKSHPKQNSRTPRSILEQGLWDTMNDSETIRSPVQSPIRRKIHR 300

Query: 301 -----TSILEQALWDTMNDSETVQSPIRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECDE 360
                 SILEQ LWDTMNDSETVQSPIRPKIHRTPLQRIQ+LSE+STIQSSG PNWECDE
Sbjct: 301 TPSQRISILEQGLWDTMNDSETVQSPIRPKIHRTPLQRIQQLSEISTIQSSGTPNWECDE 360

Query: 361 DWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMATYNGVGDQDYINVSVSRSSDYCSR 406
           DW TVRSIGLEEEDREEN+FVSVLEMPCSSQSIE A  NG  DQDYINVS S+ SDYCSR
Sbjct: 361 DWVTVRSIGLEEEDREENNFVSVLEMPCSSQSIERAINNGAVDQDYINVSDSKISDYCSR 420

[Close]

BLAST of Lsi01G004450 vs. NCBI nr
Match: TYK13030.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 702.2 bits (1811), Expect = 3.0e-198
Identity = 348/403 (86.35%), Postives = 370/403 (91.81%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRT+GRGSSAAVSVATDLRSGQ +AVK+VEF+  DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCARRVE+V GGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSILEQ +WDT+ND ETV+SPIRPKIHRTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVSTI     PNWECDEDW TVRSIGL     EEND VSV+E PCSSQS+EMA+
Sbjct: 301 RIQQLNEVSTIG----PNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            N VGDQDYINVS SRSSDYCSRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

[Close]

BLAST of Lsi01G004450 vs. NCBI nr
Match: XP_008441103.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 700.3 bits (1806), Expect = 1.1e-197
Identity = 347/403 (86.10%), Postives = 369/403 (91.56%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRT+GRGSSAAVSVATDLRSGQ +AVK+VEF+  DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTLGRGSSAAVSVATDLRSGQVMAVKTVEFSRLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTREILSGL YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDTMEKAGGRLDEATAQFYTREILSGLHYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCARRVE+V GGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILVGEDGIKIADFGCARRVEDVFGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRAPWANLSDPLAAIYRIGSGDDLPEIPRNMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSILEQ +WDT+ND ETV+SPIRPKIHRTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSNPKQNSRTPTSILEQGIWDTVNDPETVESPIRPKIHRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVST      PNWECDEDW TVRSIGL     EEND VSV+E PCSSQS+EMA+
Sbjct: 301 RIQQLNEVST----NGPNWECDEDWITVRSIGL-----EENDIVSVMETPCSSQSVEMAS 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            N VGDQDYINVS SRSSDYCSRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNEVGDQDYINVSASRSSDYCSRKSSKAKNRDFPAMSSVFNQI 394

[Close]

BLAST of Lsi01G004450 vs. NCBI nr
Match: XP_031742911.1 (mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 9.6e-197
Identity = 346/403 (85.86%), Postives = 369/403 (91.56%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEWIRGRTIGRGSSAAVSVATD+R GQ +AVKSVEF+H DFLKREQRILSQLNCS VIGY
Sbjct: 1   MEWIRGRTIGRGSSAAVSVATDIRFGQVMAVKSVEFSHLDFLKREQRILSQLNCSRVIGY 60

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENGNLMCN+LMEFAPGGSILD ME+AGGRLDEATAQ YTRE+LSGL+YVHSNG
Sbjct: 61  KGFDVTLENGNLMCNLLMEFAPGGSILDAMEKAGGRLDEATAQFYTREVLSGLQYVHSNG 120

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+L+GEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV
Sbjct: 121 VVHCDIKCCNILMGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGR PWAN+SDP+AAIYRIGSGDDLPEIPR MSEQGKDFLRRCLIR+PE
Sbjct: 181 WSVGCAVIQMVTGRVPWANLSDPLAAIYRIGSGDDLPEIPRIMSEQGKDFLRRCLIRDPE 240

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV+ELLKHPFV EQKS PKQNSRTPTSIL+Q +WDT+ND ETV+SPIRPKI RTPLQ
Sbjct: 241 ERWSVNELLKHPFVQEQKSHPKQNSRTPTSILDQGIWDTVNDPETVESPIRPKIQRTPLQ 300

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+L+EVSTI   GIPNWECDEDW TVRSIGL     EEND VSV+E P S QSI+M T
Sbjct: 301 RIQQLNEVSTI---GIPNWECDEDWITVRSIGL-----EENDIVSVMETPSSFQSIKMET 360

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQI 404
            NGVGDQDYINVS SRSSDY SRKSSKA+NRD P MSSV NQI
Sbjct: 361 NNGVGDQDYINVSRSRSSDYGSRKSSKAKNRDFPAMSSVFNQI 395

[Close]

BLAST of Lsi01G004450 vs. NCBI nr
Match: XP_023544013.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.5 bits (1734), Expect = 2.5e-189
Identity = 334/405 (82.47%), Postives = 363/405 (89.63%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           M+W RG TIGRGSSAAVSVATDL SGQ LAVKSVEFTHSDFLKREQRILS+ NC  VI Y
Sbjct: 20  MDWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTHSDFLKREQRILSRFNCPRVIAY 79

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
           KGF+VTLENG LMCN+LMEFAPGGSILDVME AGGRLDEATAQ YTREILSGLEYVHSNG
Sbjct: 80  KGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEATAQFYTREILSGLEYVHSNG 139

Query: 121 VVHCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADV 180
           VVHCDIKCCN+LVGEDGIKIADFGCAR+VEE S  NL GTP+FMAPEVARGEKQGFAADV
Sbjct: 140 VVHCDIKCCNILVGEDGIKIADFGCARQVEEDSSRNLVGTPMFMAPEVARGEKQGFAADV 199

Query: 181 WGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPE 240
           W VGCAVIQMVTGRAPW NVSDPVAAIY+IGSGDDLPE+PRFMSEQGKDFLRRCLIR+P 
Sbjct: 200 WAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPRFMSEQGKDFLRRCLIRDPG 259

Query: 241 ERWSVDELLKHPFVNEQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPIRPKIHRTPLQ 300
           ERWSV ELLKHPFV+EQKS PKQNSRTPTSILEQ  W TMN+SETVQSPI PK++RTPLQ
Sbjct: 260 ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMNNSETVQSPIMPKLYRTPLQ 319

Query: 301 RIQKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREENDFVSVLEMPCSSQSIEMAT 360
           RIQ+LSE+S IQSS IPNW+CD+DW TV+SI +EEED E+N+FVSVLE+PC SQSI+MAT
Sbjct: 320 RIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQNEFVSVLELPCFSQSIKMAT 379

Query: 361 YNGVGDQDYINVSVSRSSDYCSRKSSKAQNRDLPVMSSVCNQINS 406
            NGVGDQ+YI+VS+       SR+SSKA+ RDLP MSSV NQI S
Sbjct: 380 KNGVGDQNYIDVSI-------SRESSKAKKRDLPAMSSVSNQIVS 417

[Close]

BLAST of Lsi01G004450 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 305.1 bits (780), Expect = 9.9e-83
Identity = 159/357 (44.54%), Postives = 231/357 (64.71%), Query Frame = 0


Query: 3   WIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGYKG 62
           WIRG  IGRGS+A VS+     SG   AVKS EF+ S FL+REQ ILS+L+   ++ Y G
Sbjct: 6   WIRGPIIGRGSTATVSLGI-TNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNGVV 122
             VT EN  LM N+LME+  GGS+ D+++ +GG+L E   + YTR+IL GL Y+H  G+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKCCNLLVGEDGIKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAADVWG 182
           HCD+K  N+++G +  KI D GCA+ VEE      +GTP FM+PEVARGE+Q F ADVW 
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 VGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNPEER 242
           +GC VI+M TG +PW  ++D VAAIY+IG   + P IP ++SE+G+DFLR+CL ++P++R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSVDELLKHPFVNEQKSQPKQ-----NSRTPTSILEQALWDTMNDSETVQSPIRPKIHRT 302
           W+V+ELL+HPF++E+ +   Q     NS +P+++L+Q  WD     ET +S    + H  
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDL---CETSRSRFIKEDHED 305

Query: 303 PLQRI------------QKLSEVSTIQSSGIPNWECDEDWATVRSIGLEEEDREEND 343
           P                 ++ +++  +SSG P+WE +  W  VR   +E+ + EE++
Sbjct: 306 PFANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWETN-GWIEVRG-EIEKRNEEEDE 356

[Close]

BLAST of Lsi01G004450 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-78
Identity = 161/341 (47.21%), Postives = 218/341 (63.93%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           M W RG+T+GRGS+A VS AT   SG+ LAVKS EF  S+FL+RE +ILS LN   VIGY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  KGFEVTLE----NGNLMC-NILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEY 120
           +G E+T E    NG     ++LME+AP G++ DV  + GG +DEA    YTR+IL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 VH-SNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEK 180
           +H S G+ HCDIK  N+LVGE+G  KIADFGCA+ VE      + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGFAADVWGVGCAVIQMVTGRAPW--ANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFL 240
           QG  +D+W VGC VI+MVTG  PW  A+ +DPV+ +YR+G   +LPE+P  ++EQ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RRCLIRNPEERWSVDELLKHPFVNEQKSQPKQN--SRTPTSILEQALWDTMNDSETVQSP 300
            +CL +   ERW+  +LL HPF+  ++ +      + +PTS+ +Q  W ++ +  +    
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMFWRSVEEEVSEDRS 300

Query: 301 IRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECD-EDWATVR 330
              + H    +RI  LS +  +       W+ D EDW TVR
Sbjct: 301 SWWECHED--ERIGVLSWIGHVVVES--TWDLDGEDWITVR 337

[Close]

BLAST of Lsi01G004450 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-78
Identity = 156/350 (44.57%), Postives = 213/350 (60.86%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           MEW RGR +GRGS+A V  A    S + LAVKS E   S+FL+RE +ILS L+   VIGY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  KGFEVTLE-NGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSN 120
           +G E   E NG +M N+LME+AP G++ D   + GGR+DE     YTR+IL GLEY+HS 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GVVHCDIKCCNLLVGEDG-IKIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAA 180
           G+VHCD+K  N+++ E G  KIADFGCA+RV+ V    + GTP FMAPEVARGEKQG  +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWGVGCAVIQMVTGRAPWANV---SDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCL 240
           D+W VGC +I+MVTG  PW       DPV+ +YR+G   + PE+P  ++E+ KDFL +CL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 IRNPEERWSVDELLKHPFVN-----EQKSQPKQNSRTPTSILEQALWDTMNDSETVQSPI 300
            R   ERW+  +LL HPF+      E    P   S +PTS+ +Q  W ++ + E  ++  
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETEE 300

Query: 301 RPKIHRTPLQRI-------QKLSEVSTIQSSGIPNWECD---EDWATVRS 331
             K  R  L R+       +++  +  +   G+    CD    DW  VR+
Sbjct: 301 IQKDSR-DLDRLSLWGCYSERIGRLKCV--GGLDGTRCDMEGGDWIMVRA 347

[Close]

BLAST of Lsi01G004450 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 286.2 bits (731), Expect = 4.8e-77
Identity = 162/399 (40.60%), Postives = 253/399 (63.41%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSGQRLAVKSVEFTHSDFLKREQRILSQLNCSNVIGY 60
           + W RG  IGRGS+A VS+A    SG+  AVKS + + S  L++EQ ILS L+  +++ Y
Sbjct: 3   INWTRGPIIGRGSTATVSIAIS-SSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  KGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGRLDEATAQLYTREILSGLEYVHSNG 120
            G  +T E+  L+ NILME+  GG++ D+++ +GG+L E   + YTR+IL+GL Y+H  G
Sbjct: 63  IGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERG 122

Query: 121 VVHCDIKCCNLLVGEDGI-KIADFGCARRVEEVSGGNLAGTPIFMAPEVARGEKQGFAAD 180
           +VHCD+K  N+LV E+G+ KIAD GCA+ V++      +GTP FMAPEVARGE+Q F AD
Sbjct: 123 IVHCDLKSHNVLVEENGVLKIADMGCAKSVDK---SEFSGTPAFMAPEVARGEEQRFPAD 182

Query: 181 VWGVGCAVIQMVTGRAPWANVSDPVAAIYRIGSGDDLPEIPRFMSEQGKDFLRRCLIRNP 240
           VW +GC +I+M+TG +PW  ++D VAA+Y+IG   + P IP ++S++ KDFL+ CL  + 
Sbjct: 183 VWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQ 242

Query: 241 EERWSVDELLKHPFV-NEQKSQP----KQNSRTPTSILEQALWDTMNDSET------VQS 300
           ++RW+V+ELLKHPF+ ++++SQ     K  + +P+++L+Q  WD+   S++       + 
Sbjct: 243 KQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHLVSIDHED 302

Query: 301 PIRPKIHR--TPLQRIQKLSEVSTIQSSGIPNWECDED--WATVRSIGLEE-EDREENDF 360
           P         +P  RI+KL   +  + S + +W+ ++D  W  VR    +E E R+ ++ 
Sbjct: 303 PFAEYSESLDSPADRIEKL---AGDEFSSLLDWDTEDDGGWIQVRGEKHKETEKRDGDED 362

Query: 361 VSVLEMPCSSQSIEMATYNGVGDQDYINVSVSRSSDYCS 383
           V  +E   SSQ+IE         +D+I+   S  S+Y S
Sbjct: 363 VICVEATSSSQTIEEV-------EDWISNQDSLFSEYSS 387

[Close]

BLAST of Lsi01G004450 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 219.5 bits (558), Expect = 5.5e-57
Identity = 128/357 (35.85%), Postives = 196/357 (54.90%), Query Frame = 0


Query: 1   MEWIRGRTIGRGSSAAVSVATDLRSG----QRLAVKSVEFTHSDFLKREQRILSQL-NCS 60
           MEW+RG TIG G+ + VS AT  R+       +AVKS +   +  L  E+ +L  L +C 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NVIGYKGFEVTLENGNLMCNILMEFAPGGSILDVMEEAGGR-LDEATAQLYTREILSGLE 120
            +I   G + T+ENG  M N+L+E+A  GS+   M++ GG  L E+T + +T  +L GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YVHSNGVVHCDIKCCNLLVGEDG-IKIADFGCARRVEE-----VSGGNLAGTPIFMAPEV 180
           ++H+ G  HCDIK  N+L+  DG +KIADFG A RV+           + GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGFAADVWGVGCAVIQMVTGRAPWA--NVSDPVAAIYRIGSGDDLPEIPRFMSEQ 240
               + G AADVW +GCAV++M +G+  W+    S  ++ + RIG GD+LP+IP  +SE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRRCLIRNPEERWSVDELLKHPFVN-EQKSQPKQN-----------SRTPTSILEQ 300
           GKDFL +C +++P +RW+ + LL H FV  + +   ++N             +P    E 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 ALWD--TMNDSETVQSPIRPKIHRTPLQRIQKLSEVSTIQSSGIPNWECDEDWATVR 330
             WD  T++ + +  SP+                 + ++ S  IP+W     W TVR
Sbjct: 301 DDWDSFTLDSNPSFDSPV---------------ERLGSLVSGSIPDWSVGGSWLTVR 342

The following BLAST results are available for this feature:

BLAST of Lsi01G004450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVP5	2.7e-77	47.21	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
O80888	6.1e-77	44.57	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9SND6	7.7e-56	35.85	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q9FZ36	1.1e-49	39.86	Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702...	[more]
O22040	2.4e-49	41.48	Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi01G004450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CNL0	1.4e-198	86.35	Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw...	[more]
A0A1S3B3E3	5.5e-198	86.10	mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN...	[more]
A0A0A0KL63	4.7e-197	85.86	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513...	[more]
A0A6J1GDW5	6.3e-186	81.48	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]
A0A6J1EMC5	6.8e-180	80.34	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]

BLAST of Lsi01G004450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883085.1	2.5e-205	83.03	mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida]	[more]
TYK13030.1	3.0e-198	86.35	mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]	[more]
XP_008441103.1	1.1e-197	86.10	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
XP_031742911.1	9.6e-197	85.86	mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus]	[more]
XP_023544013.1	2.5e-189	82.47	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe...	[more]

BLAST of Lsi01G004450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55090.1	9.9e-83	44.54	mitogen-activated protein kinase kinase kinase 15	[more]
AT1G05100.1	1.9e-78	47.21	mitogen-activated protein kinase kinase kinase 18	[more]
AT2G32510.1	4.3e-78	44.57	mitogen-activated protein kinase kinase kinase 17	[more]
AT4G26890.1	4.8e-77	40.60	mitogen-activated protein kinase kinase kinase 16	[more]
AT3G50310.1	5.5e-57	35.85	mitogen-activated protein kinase kinase kinase 20	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..254 e-value: 1.5E-75 score: 266.9
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 6..254 e-value: 3.6E-52 score: 177.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 3..254 score: 40.513004
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 2..314 e-value: 1.2E-69 score: 237.0
None	No IPR available	PIRSR	PIRSR633573-1	PIRSR633573-1	coord: 7..195 e-value: 3.8E-13 score: 47.2
None	No IPR available	PIRSR	PIRSR037014-1	PIRSR037014-1	coord: 7..261 e-value: 4.6E-25 score: 85.9
None	No IPR available	PIRSR	PIRSR037993-1	PIRSR037993-1	coord: 82..265 e-value: 1.7E-18 score: 64.5
None	No IPR available	PANTHER	PTHR48011:SF6	MAP KINASE KINASE KINASE	coord: 1..356
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..356
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 2..254 e-value: 3.05006E-138 score: 395.736
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 121..133
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..32
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G004450.1	Lsi01G004450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity