Lsi01G003530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G003530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionF-box protein
Locationchr01: 3145528 .. 3147486 (+)
RNA-Seq ExpressionLsi01G003530
SyntenyLsi01G003530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTAAAGCGCGTGGGGTTTTTTAAATTTTATTTTTTACTGCTTTCTAATTTTTGAAAAGCGAGAAATCCCATCTTTTTTCACCAATATATAAAGACAGAATATCGACAATTTTAAAGCAACTCCGTAGTTTGCTGAGTTTCCATTTTAAGAGCAAAACCTTTCTTTGAAATTGAAACAGAAACAGAGTGATAGAAATATTCGAATCTCCGTAATCAGGTTCGTTTCGCTCCTGATTCAAATTTAGATTTCTTCCTTATTCGACACTTTCAATCTGCTTTTGGAAACTGTTATTAGAGCACACCTGCTTTGCTTTGCTTCGATCAGATTTTTGTTAGGGTTTTTTAACCTTTTTCAATCTCTTAATCTATGGATTCGGTTCTTTCTTTTTAGGATTTTATCAGTTCTAAATGATTGATTTTTGTTATTTTTCGCGCGTTGATACAGATAAACGAATAGATTTAATTTAATTCGAGATTTGAGAATTGACAATGGCGTTGGGAAAGAAGACCGGTTGTTTGAAATCGAAAAGCGTTGTCTCTGCTGCTGATAAAGGGCTTGATTTCGGAGTTGTGAGATATACGCGTGATTTAGGAAGGAAACGCGTTGTGATTTCTTGCAATGAGGAAGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTTGTCAGTCCGGCGGTGGACGCCAACAGGTCTCTGCTCGAGGCTTTGCCTCAGGAAATTCTGGTTAGTATCTCATACAGATTGAAATCGCTTCGATAAATGTATCTTTCATATTCGCATTCGTTCCCGTTTTCGATTATTGTTTCAGAATTGATTTTTCCCCCTTTCCTCTTTCCATCAGATTAGGGTTCTCTGCGGTGTGGAACATGATGATTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAACGAAGCGGTAAATATCACTCGAATCTCATTATTTGTTTCCTTTCATTTGATTCAATGATTCTCATCGATTTAGGGTTTCAATTACATTTACAGACTTTGATTGCAAAAGAATGGCATTTCGCTTATAGCACTCCCTCGAAGGTCCGTACATTTCGCACCGCCGTTGAATTGGACGATTCCAGCGATTTCGACGAGATTGAAGCCCCAAATGCTCCTCCCACAAGACGATTCAGAACTCCATTAAACAGAAAGAAGCTCGCGGAGATATCCGTAGCCTTATTTTCCGAAGAAGATCAGTGGCCAAAGAAAAAGTTGGCCATTGAGACAGAGATGTGAATTAACAAACCCAGAAGGCTCTGTAAATACAACCTGTATTTATATTGTTGTAGAAACACGGTTGTGGTGGTAGGAAGAAAGACGTAGTTAGGGATCTGAAATCTCAGATCTTGGTTGAAGAAGAAGCTGTACATGTCAGAACATTCTCACTGACCAATTCTTCAAATCTTTCATTGAAGATGAAGATGGGGGGTGGGTTAGAAATGCATCTATTTTTTTTGTACAGGTATTACAGAGACAGACTGATAGGTAAATTCGTTTACAAGGGTAGCATTTTGATTTAGTCCCTTCGAAATTTTCATAAACAGTACATTGTAGTATGTACTATTGCTCTCATTCATTCTCTGTCTAATAAGAACGAGCCATATTTTCCTCGCAATTTTGTGTCACTATTTACAACATAATGTTCTGCCATCCATTTGTATTGCTTCTTACTATGATTCCATTTGAATAAATTTTCCATAATTGATGAATGGGATTTTGGGTTCTCTCCTTTTCTTTCCCTTTTTGGTGTGGTGTGTGATTGAATGATTTGTAACGTATGAGAAATTAAATGGGGAAACAGAATAGAAATTATCAGAAGCATCATCAACTTTGACAGCTGTTTCTTATGCTTTTTACTCGCTAAAAAGAGTTTGAAATTCCCAATTATATGTGGAAATGTTTCTGTACAAGCTGGACTGATGAACTGGCG

mRNA sequence

TTTCTAAAGCGCGTGGGGTTTTTTAAATTTTATTTTTTACTGCTTTCTAATTTTTGAAAAGCGAGAAATCCCATCTTTTTTCACCAATATATAAAGACAGAATATCGACAATTTTAAAGCAACTCCGTAGTTTGCTGAGTTTCCATTTTAAGAGCAAAACCTTTCTTTGAAATTGAAACAGAAACAGAGTGATAGAAATATTCGAATCTCCGTAATCAGATAAACGAATAGATTTAATTTAATTCGAGATTTGAGAATTGACAATGGCGTTGGGAAAGAAGACCGGTTGTTTGAAATCGAAAAGCGTTGTCTCTGCTGCTGATAAAGGGCTTGATTTCGGAGTTGTGAGATATACGCGTGATTTAGGAAGGAAACGCGTTGTGATTTCTTGCAATGAGGAAGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTTGTCAGTCCGGCGGTGGACGCCAACAGGTCTCTGCTCGAGGCTTTGCCTCAGGAAATTCTGATTAGGGTTCTCTGCGGTGTGGAACATGATGATTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAACGAAGCGACTTTGATTGCAAAAGAATGGCATTTCGCTTATAGCACTCCCTCGAAGGTCCGTACATTTCGCACCGCCGTTGAATTGGACGATTCCAGCGATTTCGACGAGATTGAAGCCCCAAATGCTCCTCCCACAAGACGATTCAGAACTCCATTAAACAGAAAGAAGCTCGCGGAGATATCCGTAGCCTTATTTTCCGAAGAAGATCAGTGGCCAAAGAAAAAGTTGGCCATTGAGACAGAGATGTGAATTAACAAACCCAGAAGGCTCTGTAAATACAACCTGTATTTATATTGTTGTAGAAACACGGTTGTGGTGGTAGGAAGAAAGACGTAGTTAGGGATCTGAAATCTCAGATCTTGGTTGAAGAAGAAGCTGTACATGTCAGAACATTCTCACTGACCAATTCTTCAAATCTTTCATTGAAGATGAAGATGGGGGGTGGGTTAGAAATGCATCTATTTTTTTTGTACAGGTATTACAGAGACAGACTGATAGGTAAATTCGTTTACAAGGGTAGCATTTTGATTTAGTCCCTTCGAAATTTTCATAAACAGTACATTGTAGTATGTACTATTGCTCTCATTCATTCTCTGTCTAATAAGAACGAGCCATATTTTCCTCGCAATTTTGTGTCACTATTTACAACATAATGTTCTGCCATCCATTTGTATTGCTTCTTACTATGATTCCATTTGAATAAATTTTCCATAATTGATGAATGGGATTTTGGGTTCTCTCCTTTTCTTTCCCTTTTTGGTGTGGTGTGTGATTGAATGATTTGTAACGTATGAGAAATTAAATGGGGAAACAGAATAGAAATTATCAGAAGCATCATCAACTTTGACAGCTGTTTCTTATGCTTTTTACTCGCTAAAAAGAGTTTGAAATTCCCAATTATATGTGGAAATGTTTCTGTACAAGCTGGACTGATGAACTGGCG

Coding sequence (CDS)

ATGGCGTTGGGAAAGAAGACCGGTTGTTTGAAATCGAAAAGCGTTGTCTCTGCTGCTGATAAAGGGCTTGATTTCGGAGTTGTGAGATATACGCGTGATTTAGGAAGGAAACGCGTTGTGATTTCTTGCAATGAGGAAGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTTGTCAGTCCGGCGGTGGACGCCAACAGGTCTCTGCTCGAGGCTTTGCCTCAGGAAATTCTGATTAGGGTTCTCTGCGGTGTGGAACATGATGATTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAACGAAGCGACTTTGATTGCAAAAGAATGGCATTTCGCTTATAGCACTCCCTCGAAGGTCCGTACATTTCGCACCGCCGTTGAATTGGACGATTCCAGCGATTTCGACGAGATTGAAGCCCCAAATGCTCCTCCCACAAGACGATTCAGAACTCCATTAAACAGAAAGAAGCTCGCGGAGATATCCGTAGCCTTATTTTCCGAAGAAGATCAGTGGCCAAAGAAAAAGTTGGCCATTGAGACAGAGATGTGA

Protein sequence

MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKRRCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAYSTPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFSEEDQWPKKKLAIETEM
Homology
BLAST of Lsi01G003530 vs. ExPASy Swiss-Prot
Match: O65416 (F-box protein SKIP27 OS=Arabidopsis thaliana OX=3702 GN=SKIP27 PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 2.5e-29
Identity = 79/193 (40.93%), Postives = 120/193 (62.18%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGV--VRYTRDLGRKRVVI-SCNEEDSS-----IDL 60
           MAL K+   + S +     ++ L+ G+  VR+TR LGRKR++I SC  E  S     I +
Sbjct: 34  MALSKRGFVMTSNARFHGEEEELELGLGSVRFTRGLGRKRILISSCVRESLSRSAVEIPV 93

Query: 61  TPKAP--------SKRRCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSK 120
             ++P         + R ++VS +  + +S LE LPQ++LIRV+CGV+H+DLK L  VSK
Sbjct: 94  VSESPPVKSSLKRQRSRITIVSSS-SSEKSRLECLPQDLLIRVICGVDHEDLKSLKLVSK 153

Query: 121 TINEATLIAKEWHFAYSTPSKVRTFRTAVELDDSSDF----DEIEAPNAPPTRRFRTPLN 174
           +I EA+L+AK  HFAY+TP K R FR +++L++ SD     D+IE PNAP   R+     
Sbjct: 154 SIREASLVAKTLHFAYTTPKKTRAFRNSIDLEEVSDSRHQEDDIEPPNAPRHYRWTKAKR 213

BLAST of Lsi01G003530 vs. ExPASy Swiss-Prot
Match: Q8GX77 (F-box protein At1g61340 OS=Arabidopsis thaliana OX=3702 GN=At1g61340 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 83/186 (44.62%), Postives = 109/186 (58.60%), Query Frame = 0

Query: 1   MALGKK-----TGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEE-DSSIDLTP 60
           MALGKK        L+ +  V     GL    V+Y R  GRKR++IS  +E + SI  +P
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEFVQYKRGFGRKRILISSGDEMEDSIFTSP 60

Query: 61  KAPSKRRCSVVSPAVDANRSL-LEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIA 120
               K+ C   + +V   +S  LE LP +IL+R++CGVEH+DLKQL  VSKTI EAT+IA
Sbjct: 61  --VGKKLCDDKTTSVAEGQSRELEDLPLDILVRIICGVEHEDLKQLFHVSKTIREATMIA 120

Query: 121 KEWHFAYSTPSKVRTFRTA-----VELDDSSDFDEIEAPNAPPTRRFR-TPLNRKK-LAE 173
           K+ HFAYSTP K   F           D   D +EIEAP AP  +R+R + +NR K  + 
Sbjct: 121 KQSHFAYSTPRKTSVFHHGRFGWDKPFDVEDDDEEIEAPGAPLQKRYRLSRINRNKDDSG 180

BLAST of Lsi01G003530 vs. ExPASy Swiss-Prot
Match: Q9S9T6 (F-box protein At4g05010 OS=Arabidopsis thaliana OX=3702 GN=At4g05010 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 76/158 (48.10%), Postives = 94/158 (59.49%), Query Frame = 0

Query: 23  LDFGVVRYTRDLGRKRVVIS--CNEEDSSIDLTPKAPSKRRCSVVSPAVDANRSLLEALP 82
           L  G VR+TR LGRKR++IS    E DS     P   +   C          RSLLE L 
Sbjct: 19  LGLGFVRFTRGLGRKRILISKRAPENDSPPVKRPSHETTESC----------RSLLETLH 78

Query: 83  QEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAYSTPSKVRTFRTAVELDD--- 142
           Q+ILIRVLC V+H+DL  L RVSKTI +A + AK+ HF YSTP K   FR A+ + D   
Sbjct: 79  QDILIRVLCHVDHEDLATLKRVSKTIRKAVIEAKKSHFDYSTPKKRLPFRDAILILDSNS 138

Query: 143 --SSDFDEIEAPNAPPTRRFRTPLNRKK-LAEISVALF 173
             SS  DE+E PNAP  RRF   +NR+  L++IS+ LF
Sbjct: 139 NSSSQDDEMEPPNAPIRRRF---INRESDLSKISMVLF 163

BLAST of Lsi01G003530 vs. ExPASy Swiss-Prot
Match: Q5XF11 (F-box protein At4g35930 OS=Arabidopsis thaliana OX=3702 GN=At4g35930 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.8e-10
Identity = 42/143 (29.37%), Postives = 73/143 (51.05%), Query Frame = 0

Query: 53  TPKAPSKRRCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLI 112
           TPK P    C         + S LE+LP ++L++++C + HD LK +  VS+ I  AT++
Sbjct: 147 TPKTPQADPC--------VSESQLESLPMDLLVKIVCHLHHDQLKAVFHVSQRIRMATIL 206

Query: 113 AKEWHFAYSTPSKVR----TFRTAVELD------DSSDFDEIEAPNAPPT-RRFRTPLNR 172
           A+++HF Y+TP + R       T V ++         +   + +P+ P   +    P +R
Sbjct: 207 ARQYHFNYTTPDRSRQEMLRVMTPVPINRWPFRRGDGNPTMVSSPHTPKAPKHAPRPPSR 266

Query: 173 KKLAE---ISVALFSEEDQWPKK 182
            KLAE   I+  LF ++  +P +
Sbjct: 267 TKLAEMKQITAVLFQDQTPFPSR 281

BLAST of Lsi01G003530 vs. ExPASy TrEMBL
Match: A0A5A7UGT0 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003340 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.6e-87
Identity = 171/190 (90.00%), Postives = 182/190 (95.79%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISCNEEDSSIDLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RC+VVS AV+A+RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTI+EATLIAK+ HFAY
Sbjct: 61  RCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+EL+DSSDFDEIEAPNA PTRRFR+PLNRKKLAEISVALF+ EED+WP
Sbjct: 121 STPSKVRAFRTAIELNDSSDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDEWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. ExPASy TrEMBL
Match: A0A5D3CPZ7 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007130 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.6e-87
Identity = 171/190 (90.00%), Postives = 181/190 (95.26%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISCNEEDSSIDLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
            C+VVS AV+A+RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTI+EATLIAK+ HFAY
Sbjct: 61  HCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+ELDDSSDFDEIEAPNA PTRRFR+PLNRKKLAEISVALF+ EED+WP
Sbjct: 121 STPSKVRAFRTAIELDDSSDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDEWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. ExPASy TrEMBL
Match: A0A1S3B0I0 (F-box protein At4g05010-like OS=Cucumis melo OX=3656 GN=LOC103484553 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.6e-87
Identity = 171/190 (90.00%), Postives = 182/190 (95.79%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISCNEEDSSIDLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RC+VVS AV+A+RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTI+EATLIAK+ HFAY
Sbjct: 61  RCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+EL+DSSDFDEIEAPNA PTRRFR+PLNRKKLAEISVALF+ EED+WP
Sbjct: 121 STPSKVRAFRTAIELNDSSDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDEWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. ExPASy TrEMBL
Match: A0A0A0KI01 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516960 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.0e-86
Identity = 170/190 (89.47%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISC+EEDS  DLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCHEEDSPTDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RC+VVS AV+ +RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTINEATLIAK+ HFAY
Sbjct: 61  RCNVVSTAVEDDRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTINEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+ELDDSSDFDEIEAPNA PTRRFRTPLNRKKLAEIS+ALF+ EEDQWP
Sbjct: 121 STPSKVRRFRTAIELDDSSDFDEIEAPNACPTRRFRTPLNRKKLAEISIALFAEEEDQWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. ExPASy TrEMBL
Match: A0A6J1IRQ4 (F-box protein SKIP27-like OS=Cucurbita maxima OX=3661 GN=LOC111478110 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.7e-81
Identity = 157/190 (82.63%), Postives = 174/190 (91.58%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKK+GCLKSKSVVSAADK LDFGVVRY R LGRKRVVISCN+EDSSIDLTPK PSKR
Sbjct: 1   MALGKKSGCLKSKSVVSAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKTPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RCSVVS  VDA  SLLE LPQEILIR+LCG++HDDLKQLVRVSKTI+EATLIAKEWHFAY
Sbjct: 61  RCSVVSTTVDAGWSLLEVLPQEILIRILCGLDHDDLKQLVRVSKTISEATLIAKEWHFAY 120

Query: 121 STPSKVRTFRTAVELDDS-SDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFSEEDQWP 180
            TPSK+R FRT++ELDD+  D DEIEAP+AP TRRFR+PL+RKKLA+ISVALF+EE+QWP
Sbjct: 121 RTPSKIRAFRTSIELDDNFIDLDEIEAPDAPLTRRFRSPLSRKKLADISVALFAEEEQWP 180

Query: 181 KKKLAIETEM 190
           +KK AIETEM
Sbjct: 181 RKKYAIETEM 190

BLAST of Lsi01G003530 vs. NCBI nr
Match: XP_008439907.1 (PREDICTED: F-box protein At4g05010-like [Cucumis melo] >KAA0052721.1 F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 332.0 bits (850), Expect = 3.3e-87
Identity = 171/190 (90.00%), Postives = 182/190 (95.79%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISCNEEDSSIDLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RC+VVS AV+A+RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTI+EATLIAK+ HFAY
Sbjct: 61  RCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+EL+DSSDFDEIEAPNA PTRRFR+PLNRKKLAEISVALF+ EED+WP
Sbjct: 121 STPSKVRAFRTAIELNDSSDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDEWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. NCBI nr
Match: TYK13104.1 (F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 332.0 bits (850), Expect = 3.3e-87
Identity = 171/190 (90.00%), Postives = 181/190 (95.26%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISCNEEDSSIDLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
            C+VVS AV+A+RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTI+EATLIAK+ HFAY
Sbjct: 61  HCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+ELDDSSDFDEIEAPNA PTRRFR+PLNRKKLAEISVALF+ EED+WP
Sbjct: 121 STPSKVRAFRTAIELDDSSDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDEWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. NCBI nr
Match: XP_004134730.1 (F-box protein At4g05010 [Cucumis sativus] >XP_031744056.1 F-box protein At4g05010 [Cucumis sativus] >KGN49188.1 hypothetical protein Csa_003153 [Cucumis sativus])

HSP 1 Score: 329.3 bits (843), Expect = 2.1e-86
Identity = 170/190 (89.47%), Postives = 179/190 (94.21%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKKTGCLKSKSVVSAADKGLDFGVVRYTR LGRKRVVISC+EEDS  DLTPKAPSKR
Sbjct: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRGLGRKRVVISCHEEDSPTDLTPKAPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RC+VVS AV+ +RSLLEALPQEILIRVLCGVEHDDLKQL+RVSKTINEATLIAK+ HFAY
Sbjct: 61  RCNVVSTAVEDDRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTINEATLIAKDSHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFS-EEDQWP 180
           STPSKVR FRTA+ELDDSSDFDEIEAPNA PTRRFRTPLNRKKLAEIS+ALF+ EEDQWP
Sbjct: 121 STPSKVRRFRTAIELDDSSDFDEIEAPNACPTRRFRTPLNRKKLAEISIALFAEEEDQWP 180

Query: 181 KKKLAIETEM 190
           KKKL I TEM
Sbjct: 181 KKKLPIWTEM 190

BLAST of Lsi01G003530 vs. NCBI nr
Match: XP_038880993.1 (F-box protein SKIP27 [Benincasa hispida])

HSP 1 Score: 323.2 bits (827), Expect = 1.5e-84
Identity = 166/190 (87.37%), Postives = 178/190 (93.68%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKK+GCLKSK+VVSA DKGLDFGVVRYTR LGRKRVVIS NEEDSSIDLTPKA SKR
Sbjct: 1   MALGKKSGCLKSKTVVSATDKGLDFGVVRYTRGLGRKRVVISYNEEDSSIDLTPKAQSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RCSVVS AVD++RSLLEALPQEILI+VLCGVEHDDLKQLVRVSKTI+EATL+AKEWHFAY
Sbjct: 61  RCSVVSTAVDSDRSLLEALPQEILIKVLCGVEHDDLKQLVRVSKTISEATLVAKEWHFAY 120

Query: 121 STPSKVRTFRTAVELDDSSDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALF-SEEDQWP 180
           +TPSKVRTFRTA+ELDDSSD+DEIEAP AP TRR RTPLNRKKLAEISVALF  EE+QWP
Sbjct: 121 NTPSKVRTFRTAIELDDSSDYDEIEAPYAPHTRRIRTPLNRKKLAEISVALFVEEENQWP 180

Query: 181 KKKLAIETEM 190
           KKKL IE E+
Sbjct: 181 KKKLPIEREV 190

BLAST of Lsi01G003530 vs. NCBI nr
Match: XP_022977983.1 (F-box protein SKIP27-like [Cucurbita maxima])

HSP 1 Score: 312.0 bits (798), Expect = 3.5e-81
Identity = 157/190 (82.63%), Postives = 174/190 (91.58%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEEDSSIDLTPKAPSKR 60
           MALGKK+GCLKSKSVVSAADK LDFGVVRY R LGRKRVVISCN+EDSSIDLTPK PSKR
Sbjct: 1   MALGKKSGCLKSKSVVSAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKTPSKR 60

Query: 61  RCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAY 120
           RCSVVS  VDA  SLLE LPQEILIR+LCG++HDDLKQLVRVSKTI+EATLIAKEWHFAY
Sbjct: 61  RCSVVSTTVDAGWSLLEVLPQEILIRILCGLDHDDLKQLVRVSKTISEATLIAKEWHFAY 120

Query: 121 STPSKVRTFRTAVELDDS-SDFDEIEAPNAPPTRRFRTPLNRKKLAEISVALFSEEDQWP 180
            TPSK+R FRT++ELDD+  D DEIEAP+AP TRRFR+PL+RKKLA+ISVALF+EE+QWP
Sbjct: 121 RTPSKIRAFRTSIELDDNFIDLDEIEAPDAPLTRRFRSPLSRKKLADISVALFAEEEQWP 180

Query: 181 KKKLAIETEM 190
           +KK AIETEM
Sbjct: 181 RKKYAIETEM 190

BLAST of Lsi01G003530 vs. TAIR 10
Match: AT4G21510.1 (F-box family protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.8e-30
Identity = 79/193 (40.93%), Postives = 120/193 (62.18%), Query Frame = 0

Query: 1   MALGKKTGCLKSKSVVSAADKGLDFGV--VRYTRDLGRKRVVI-SCNEEDSS-----IDL 60
           MAL K+   + S +     ++ L+ G+  VR+TR LGRKR++I SC  E  S     I +
Sbjct: 34  MALSKRGFVMTSNARFHGEEEELELGLGSVRFTRGLGRKRILISSCVRESLSRSAVEIPV 93

Query: 61  TPKAP--------SKRRCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSK 120
             ++P         + R ++VS +  + +S LE LPQ++LIRV+CGV+H+DLK L  VSK
Sbjct: 94  VSESPPVKSSLKRQRSRITIVSSS-SSEKSRLECLPQDLLIRVICGVDHEDLKSLKLVSK 153

Query: 121 TINEATLIAKEWHFAYSTPSKVRTFRTAVELDDSSDF----DEIEAPNAPPTRRFRTPLN 174
           +I EA+L+AK  HFAY+TP K R FR +++L++ SD     D+IE PNAP   R+     
Sbjct: 154 SIREASLVAKTLHFAYTTPKKTRAFRNSIDLEEVSDSRHQEDDIEPPNAPRHYRWTKAKR 213

BLAST of Lsi01G003530 vs. TAIR 10
Match: AT1G61340.1 (F-box family protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 83/186 (44.62%), Postives = 109/186 (58.60%), Query Frame = 0

Query: 1   MALGKK-----TGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEE-DSSIDLTP 60
           MALGKK        L+ +  V     GL    V+Y R  GRKR++IS  +E + SI  +P
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEFVQYKRGFGRKRILISSGDEMEDSIFTSP 60

Query: 61  KAPSKRRCSVVSPAVDANRSL-LEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLIA 120
               K+ C   + +V   +S  LE LP +IL+R++CGVEH+DLKQL  VSKTI EAT+IA
Sbjct: 61  --VGKKLCDDKTTSVAEGQSRELEDLPLDILVRIICGVEHEDLKQLFHVSKTIREATMIA 120

Query: 121 KEWHFAYSTPSKVRTFRTA-----VELDDSSDFDEIEAPNAPPTRRFR-TPLNRKK-LAE 173
           K+ HFAYSTP K   F           D   D +EIEAP AP  +R+R + +NR K  + 
Sbjct: 121 KQSHFAYSTPRKTSVFHHGRFGWDKPFDVEDDDEEIEAPGAPLQKRYRLSRINRNKDDSG 180

BLAST of Lsi01G003530 vs. TAIR 10
Match: AT4G05010.1 (F-box family protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.2e-25
Identity = 76/158 (48.10%), Postives = 94/158 (59.49%), Query Frame = 0

Query: 23  LDFGVVRYTRDLGRKRVVIS--CNEEDSSIDLTPKAPSKRRCSVVSPAVDANRSLLEALP 82
           L  G VR+TR LGRKR++IS    E DS     P   +   C          RSLLE L 
Sbjct: 19  LGLGFVRFTRGLGRKRILISKRAPENDSPPVKRPSHETTESC----------RSLLETLH 78

Query: 83  QEILIRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAYSTPSKVRTFRTAVELDD--- 142
           Q+ILIRVLC V+H+DL  L RVSKTI +A + AK+ HF YSTP K   FR A+ + D   
Sbjct: 79  QDILIRVLCHVDHEDLATLKRVSKTIRKAVIEAKKSHFDYSTPKKRLPFRDAILILDSNS 138

Query: 143 --SSDFDEIEAPNAPPTRRFRTPLNRKK-LAEISVALF 173
             SS  DE+E PNAP  RRF   +NR+  L++IS+ LF
Sbjct: 139 NSSSQDDEMEPPNAPIRRRF---INRESDLSKISMVLF 163

BLAST of Lsi01G003530 vs. TAIR 10
Match: AT1G61340.2 (F-box family protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-21
Identity = 83/232 (35.78%), Postives = 109/232 (46.98%), Query Frame = 0

Query: 1   MALGKK-----TGCLKSKSVVSAADKGLDFGVVRYTRDLGRKRVVISCNEE-DSSIDLTP 60
           MALGKK        L+ +  V     GL    V+Y R  GRKR++IS  +E + SI  +P
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEFVQYKRGFGRKRILISSGDEMEDSIFTSP 60

Query: 61  KAPSKRRCSVVSPAVDANRSL-LEALPQEIL----------------------------- 120
               K+ C   + +V   +S  LE LP +IL                             
Sbjct: 61  --VGKKLCDDKTTSVAEGQSRELEDLPLDILLRVFVNSASVNQIELKVESFLFSEIQNRN 120

Query: 121 -----------------IRVLCGVEHDDLKQLVRVSKTINEATLIAKEWHFAYSTPSKVR 173
                            +R++CGVEH+DLKQL  VSKTI EAT+IAK+ HFAYSTP K  
Sbjct: 121 GNEKMFNFFHIWKTFVIVRIICGVEHEDLKQLFHVSKTIREATMIAKQSHFAYSTPRKTS 180

BLAST of Lsi01G003530 vs. TAIR 10
Match: AT4G35930.1 (F-box family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.1e-11
Identity = 42/143 (29.37%), Postives = 73/143 (51.05%), Query Frame = 0

Query: 53  TPKAPSKRRCSVVSPAVDANRSLLEALPQEILIRVLCGVEHDDLKQLVRVSKTINEATLI 112
           TPK P    C         + S LE+LP ++L++++C + HD LK +  VS+ I  AT++
Sbjct: 147 TPKTPQADPC--------VSESQLESLPMDLLVKIVCHLHHDQLKAVFHVSQRIRMATIL 206

Query: 113 AKEWHFAYSTPSKVR----TFRTAVELD------DSSDFDEIEAPNAPPT-RRFRTPLNR 172
           A+++HF Y+TP + R       T V ++         +   + +P+ P   +    P +R
Sbjct: 207 ARQYHFNYTTPDRSRQEMLRVMTPVPINRWPFRRGDGNPTMVSSPHTPKAPKHAPRPPSR 266

Query: 173 KKLAE---ISVALFSEEDQWPKK 182
            KLAE   I+  LF ++  +P +
Sbjct: 267 TKLAEMKQITAVLFQDQTPFPSR 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O654162.5e-2940.93F-box protein SKIP27 OS=Arabidopsis thaliana OX=3702 GN=SKIP27 PE=1 SV=2[more]
Q8GX771.5e-2644.62F-box protein At1g61340 OS=Arabidopsis thaliana OX=3702 GN=At1g61340 PE=2 SV=1[more]
Q9S9T63.2e-2448.10F-box protein At4g05010 OS=Arabidopsis thaliana OX=3702 GN=At4g05010 PE=2 SV=1[more]
Q5XF115.8e-1029.37F-box protein At4g35930 OS=Arabidopsis thaliana OX=3702 GN=At4g35930 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UGT01.6e-8790.00F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003340... [more]
A0A5D3CPZ71.6e-8790.00F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007130... [more]
A0A1S3B0I01.6e-8790.00F-box protein At4g05010-like OS=Cucumis melo OX=3656 GN=LOC103484553 PE=4 SV=1[more]
A0A0A0KI011.0e-8689.47F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516960 PE=4 ... [more]
A0A6J1IRQ41.7e-8182.63F-box protein SKIP27-like OS=Cucurbita maxima OX=3661 GN=LOC111478110 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008439907.13.3e-8790.00PREDICTED: F-box protein At4g05010-like [Cucumis melo] >KAA0052721.1 F-box prote... [more]
TYK13104.13.3e-8790.00F-box protein [Cucumis melo var. makuwa][more]
XP_004134730.12.1e-8689.47F-box protein At4g05010 [Cucumis sativus] >XP_031744056.1 F-box protein At4g0501... [more]
XP_038880993.11.5e-8487.37F-box protein SKIP27 [Benincasa hispida][more]
XP_022977983.13.5e-8182.63F-box protein SKIP27-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G21510.11.8e-3040.93F-box family protein [more]
AT1G61340.11.1e-2744.62F-box family protein [more]
AT4G05010.12.2e-2548.10F-box family protein [more]
AT1G61340.24.4e-2135.78F-box family protein [more]
AT4G35930.14.1e-1129.37F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34049:SF1F-BOX PROTEIN SKIP27coord: 5..176
NoneNo IPR availablePANTHERPTHR34049F-BOX PROTEIN SKIP27coord: 5..176
IPR001810F-box domainPROSITEPS50181FBOXcoord: 73..123
score: 8.994076
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 71..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G003530.1Lsi01G003530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding