Lsi01G003140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G003140
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr01: 2780525 .. 2782366 (+)
RNA-Seq ExpressionLsi01G003140
SyntenyLsi01G003140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAAGAAAATTCATCTTCCAGAAATTATCAATCCAATTATGAACAAAATTATACTGGAAACCGAACAACTTGCATTTCAACATCCTGTTACTCGCATTTTTGACGCGCGATTGCACTCTCCAGTCTACGAAGCGCTTCTTCAAGCAGGTCCCCGTCTGAGAAACCTTCAACAAGTTCATGCCCATATTATCGTTTCCGGACTTCAAAGAAGTCGATCCCTCCTCACTAAGCTTCTTTCTCTGGTTTGTGCCGCTGGTTCAATCACCTATGCTCGGCGATTGTTCCCCACTGTGCCAAATCCTGACTCTTTCCTGTTTGATTCCCTCCTCAAAGCGACTTCCAAATTCGGTTTCTCTATTGATGCCGTCTTGTTTTACCGTCATATGCTTTTCTCGGGTGTTCCCCGGTCGAATTATACTTTTACGTCCGTGATCAAAGCCTGTGCAGATCTTTCAGCTCTGAGGTTGGGTAGAGAAATTCATTCTCATGTTATGGTTTGTGGGTATGGTTCTGATATGTATGTCCAGGCTGCACTAGTTGCTCTCTATGCCAAAGCTAGTGATATGAAAGTTGCCAAGAAGGTGTTTGATGTAATGCCAGAAAGAACAATTATAGCTTGGAACTCACTTATATCAGGGTACGAGCAAAATGGATTCCCGAAAGAATCTATTGGTGTATTTCATCTGATGTTGGAGTCGGGCTTTCAACCTGATCCAACGACAATAGTGAGCTTATTGTCTTCCTGTTCTCAGCTAGGGGCTCTTGATTTCGGTTGCTGGTTGCATGATTATGCTAATGGTAAGGGTTTTGATCTCAATGTGGTTCTTGGTACTTCATTGATTAACATGTACACTAGATGTGGGAATGTAAGCAAAGCGCGGGAAGTTTTTGACTCCATGAAGGAAAGGAATGTTGTTACCTGGACAGCCATGATTTCAGGGTACGGGATGCATGGCTACGGTGAGCAAGCAATGAAGCTTTTTGGTGAAATGAGAGCTTTTGGTCCTCGCCCTAACAATATTACATTCGTTGCAGTCTTGTCTGCATGTGCTCATTCAGGGTTGATAGATGATGGCCGTGGGGTATTTTCAAGCATAAAGGAAGCATATGGGTTAGTTCCAGGAGTAGAACACCATGTCTGCATGGTAGATATGTTCGGCCGTGCCGGATTGCTCAACGATGCTTATCAATTTATCAAAAAATTTGTTCCTAAAGAGCCAGGTCCGGCAGTTTGGACTTCAATGCTTGGGGCTTGCAGAATGCATAAAAATTTTGACCTTGGAGTTAAGGTTGCAGAACATGTTTTAGCAGTTGAGCCAGAAAACCCCGGTCATTATGTAATGCTTTCTAACATATATGCATTGGCCGGTAGGATGGATCGAGTGGAGATGGTACGAAATATGATGATTAGACGACGCCTAAAGAAGCAAGTAGGCTATAGCACCATAGAGATCAAGCGAAAGACATATTTGTTTAGCATGGGTGACAAGTCTCACCCTCAGACAAACACAATCTATAGGTATTTAGATGAATTGATGGGTCGATGTAGTGAATCAGGCTATGTACCAGCACTAGAGTCCTTGATGCATGATTTGGAAGATGAGGAAAGGGATTATGCCCTTAGGTATCATAGTGAGAAGCTTGCACTAGCTTTTGGTTTACTTGAAACCAATCAAGGTGACACTATAAGGATTGTAAAGAACCTTCGAATATGTGAGGATTGTCATTCAGCTATTAAACACGTCTCTATTATTGCTGATAGAGAAATAATTGTTAGGGATAAATTTCGTTTTCACCACTTCAAAGATGGTGCATGTTCTTGTCTTGATTATTGGTGA

mRNA sequence

ATGCAGAAGAAAATTCATCTTCCAGAAATTATCAATCCAATTATGAACAAAATTATACTGGAAACCGAACAACTTGCATTTCAACATCCTGTTACTCGCATTTTTGACGCGCGATTGCACTCTCCAGTCTACGAAGCGCTTCTTCAAGCAGGTCCCCGTCTGAGAAACCTTCAACAAGTTCATGCCCATATTATCGTTTCCGGACTTCAAAGAAGTCGATCCCTCCTCACTAAGCTTCTTTCTCTGGTTTGTGCCGCTGGTTCAATCACCTATGCTCGGCGATTGTTCCCCACTGTGCCAAATCCTGACTCTTTCCTGTTTGATTCCCTCCTCAAAGCGACTTCCAAATTCGGTTTCTCTATTGATGCCGTCTTGTTTTACCGTCATATGCTTTTCTCGGGTGTTCCCCGGTCGAATTATACTTTTACGTCCGTGATCAAAGCCTGTGCAGATCTTTCAGCTCTGAGGTTGGGTAGAGAAATTCATTCTCATGTTATGGTTTGTGGGTATGGTTCTGATATGTATGTCCAGGCTGCACTAGTTGCTCTCTATGCCAAAGCTAGTGATATGAAAGTTGCCAAGAAGGTGTTTGATGTAATGCCAGAAAGAACAATTATAGCTTGGAACTCACTTATATCAGGGTACGAGCAAAATGGATTCCCGAAAGAATCTATTGGTGTATTTCATCTGATGTTGGAGTCGGGCTTTCAACCTGATCCAACGACAATAGTGAGCTTATTGTCTTCCTGTTCTCAGCTAGGGGCTCTTGATTTCGGTTGCTGGTTGCATGATTATGCTAATGGTAAGGGTTTTGATCTCAATGTGGTTCTTGGTACTTCATTGATTAACATGTACACTAGATGTGGGAATGTAAGCAAAGCGCGGGAAGTTTTTGACTCCATGAAGGAAAGGAATGTTGTTACCTGGACAGCCATGATTTCAGGGTACGGGATGCATGGCTACGGTGAGCAAGCAATGAAGCTTTTTGGTGAAATGAGAGCTTTTGGTCCTCGCCCTAACAATATTACATTCGTTGCAGTCTTGTCTGCATGTGCTCATTCAGGGTTGATAGATGATGGCCGTGGGGTATTTTCAAGCATAAAGGAAGCATATGGGTTAGTTCCAGGAGTAGAACACCATGTCTGCATGGTAGATATGTTCGGCCGTGCCGGATTGCTCAACGATGCTTATCAATTTATCAAAAAATTTGTTCCTAAAGAGCCAGGTCCGGCAGTTTGGACTTCAATGCTTGGGGCTTGCAGAATGCATAAAAATTTTGACCTTGGAGTTAAGGTTGCAGAACATGTTTTAGCAGTTGAGCCAGAAAACCCCGGTCATTATGTAATGCTTTCTAACATATATGCATTGGCCGGTAGGATGGATCGAGTGGAGATGGTACGAAATATGATGATTAGACGACGCCTAAAGAAGCAAGTAGGCTATAGCACCATAGAGATCAAGCGAAAGACATATTTGTTTAGCATGGGTGACAAGTCTCACCCTCAGACAAACACAATCTATAGGTATTTAGATGAATTGATGGGTCGATGTAGTGAATCAGGCTATGTACCAGCACTAGAGTCCTTGATGCATGATTTGGAAGATGAGGAAAGGGATTATGCCCTTAGGTATCATAGTGAGAAGCTTGCACTAGCTTTTGGTTTACTTGAAACCAATCAAGGTGACACTATAAGGATTGTAAAGAACCTTCGAATATGTGAGGATTGTCATTCAGCTATTAAACACGTCTCTATTATTGCTGATAGAGAAATAATTGTTAGGGATAAATTTCGTTTTCACCACTTCAAAGATGGTGCATGTTCTTGTCTTGATTATTGGTGA

Coding sequence (CDS)

ATGCAGAAGAAAATTCATCTTCCAGAAATTATCAATCCAATTATGAACAAAATTATACTGGAAACCGAACAACTTGCATTTCAACATCCTGTTACTCGCATTTTTGACGCGCGATTGCACTCTCCAGTCTACGAAGCGCTTCTTCAAGCAGGTCCCCGTCTGAGAAACCTTCAACAAGTTCATGCCCATATTATCGTTTCCGGACTTCAAAGAAGTCGATCCCTCCTCACTAAGCTTCTTTCTCTGGTTTGTGCCGCTGGTTCAATCACCTATGCTCGGCGATTGTTCCCCACTGTGCCAAATCCTGACTCTTTCCTGTTTGATTCCCTCCTCAAAGCGACTTCCAAATTCGGTTTCTCTATTGATGCCGTCTTGTTTTACCGTCATATGCTTTTCTCGGGTGTTCCCCGGTCGAATTATACTTTTACGTCCGTGATCAAAGCCTGTGCAGATCTTTCAGCTCTGAGGTTGGGTAGAGAAATTCATTCTCATGTTATGGTTTGTGGGTATGGTTCTGATATGTATGTCCAGGCTGCACTAGTTGCTCTCTATGCCAAAGCTAGTGATATGAAAGTTGCCAAGAAGGTGTTTGATGTAATGCCAGAAAGAACAATTATAGCTTGGAACTCACTTATATCAGGGTACGAGCAAAATGGATTCCCGAAAGAATCTATTGGTGTATTTCATCTGATGTTGGAGTCGGGCTTTCAACCTGATCCAACGACAATAGTGAGCTTATTGTCTTCCTGTTCTCAGCTAGGGGCTCTTGATTTCGGTTGCTGGTTGCATGATTATGCTAATGGTAAGGGTTTTGATCTCAATGTGGTTCTTGGTACTTCATTGATTAACATGTACACTAGATGTGGGAATGTAAGCAAAGCGCGGGAAGTTTTTGACTCCATGAAGGAAAGGAATGTTGTTACCTGGACAGCCATGATTTCAGGGTACGGGATGCATGGCTACGGTGAGCAAGCAATGAAGCTTTTTGGTGAAATGAGAGCTTTTGGTCCTCGCCCTAACAATATTACATTCGTTGCAGTCTTGTCTGCATGTGCTCATTCAGGGTTGATAGATGATGGCCGTGGGGTATTTTCAAGCATAAAGGAAGCATATGGGTTAGTTCCAGGAGTAGAACACCATGTCTGCATGGTAGATATGTTCGGCCGTGCCGGATTGCTCAACGATGCTTATCAATTTATCAAAAAATTTGTTCCTAAAGAGCCAGGTCCGGCAGTTTGGACTTCAATGCTTGGGGCTTGCAGAATGCATAAAAATTTTGACCTTGGAGTTAAGGTTGCAGAACATGTTTTAGCAGTTGAGCCAGAAAACCCCGGTCATTATGTAATGCTTTCTAACATATATGCATTGGCCGGTAGGATGGATCGAGTGGAGATGGTACGAAATATGATGATTAGACGACGCCTAAAGAAGCAAGTAGGCTATAGCACCATAGAGATCAAGCGAAAGACATATTTGTTTAGCATGGGTGACAAGTCTCACCCTCAGACAAACACAATCTATAGGTATTTAGATGAATTGATGGGTCGATGTAGTGAATCAGGCTATGTACCAGCACTAGAGTCCTTGATGCATGATTTGGAAGATGAGGAAAGGGATTATGCCCTTAGGTATCATAGTGAGAAGCTTGCACTAGCTTTTGGTTTACTTGAAACCAATCAAGGTGACACTATAAGGATTGTAAAGAACCTTCGAATATGTGAGGATTGTCATTCAGCTATTAAACACGTCTCTATTATTGCTGATAGAGAAATAATTGTTAGGGATAAATTTCGTTTTCACCACTTCAAAGATGGTGCATGTTCTTGTCTTGATTATTGGTGA

Protein sequence

MQKKIHLPEIINPIMNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW
Homology
BLAST of Lsi01G003140 vs. ExPASy Swiss-Prot
Match: P93011 (Pentatricopeptide repeat-containing protein At2g33760 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H6 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 5.5e-219
Identity = 353/577 (61.18%), Postives = 460/577 (79.72%), Query Frame = 0

Query: 40  HSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTV 99
           +S  YEA+++AGPR++ LQQVHAH+IV+G  RSRSLLTKL++L C+A +I Y   LF +V
Sbjct: 8   NSAAYEAIVRAGPRVKQLQQVHAHLIVTGYGRSRSLLTKLITLACSARAIAYTHLLFLSV 67

Query: 100 PNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSALRLGR 159
           P PD FLF+S++K+TSK    +  V +YR ML S V  SNYTFTSVIK+CADLSALR+G+
Sbjct: 68  PLPDDFLFNSVIKSTSKLRLPLHCVAYYRRMLSSNVSPSNYTFTSVIKSCADLSALRIGK 127

Query: 160 EIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNG 219
            +H H +V G+G D YVQAALV  Y+K  DM+ A++VFD MPE++I+AWNSL+SG+EQNG
Sbjct: 128 GVHCHAVVSGFGLDTYVQAALVTFYSKCGDMEGARQVFDRMPEKSIVAWNSLVSGFEQNG 187

Query: 220 FPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGT 279
              E+I VF+ M ESGF+PD  T VSLLS+C+Q GA+  G W+H Y   +G DLNV LGT
Sbjct: 188 LADEAIQVFYQMRESGFEPDSATFVSLLSACAQTGAVSLGSWVHQYIISEGLDLNVKLGT 247

Query: 280 SLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMR-AFGPR 339
           +LIN+Y+RCG+V KAREVFD MKE NV  WTAMIS YG HGYG+QA++LF +M    GP 
Sbjct: 248 ALINLYSRCGDVGKAREVFDKMKETNVAAWTAMISAYGTHGYGQQAVELFNKMEDDCGPI 307

Query: 340 PNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQ 399
           PNN+TFVAVLSACAH+GL+++GR V+  + ++Y L+PGVEHHVCMVDM GRAG L++AY+
Sbjct: 308 PNNVTFVAVLSACAHAGLVEEGRSVYKRMTKSYRLIPGVEHHVCMVDMLGRAGFLDEAYK 367

Query: 400 FIKKF--VPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYAL 459
           FI +     K   PA+WT+MLGAC+MH+N+DLGV++A+ ++A+EP+NPGH+VMLSNIYAL
Sbjct: 368 FIHQLDATGKATAPALWTAMLGACKMHRNYDLGVEIAKRLIALEPDNPGHHVMLSNIYAL 427

Query: 460 AGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGR 519
           +G+ D V  +R+ M+R  L+KQVGYS IE++ KTY+FSMGD+SH +T  IYRYL+ L+ R
Sbjct: 428 SGKTDEVSHIRDGMMRNNLRKQVGYSVIEVENKTYMFSMGDESHQETGEIYRYLETLISR 487

Query: 520 CSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDC 579
           C E GY P  E +MH +E+EE+++ALRYHSEKLA+AFGLL+T     I IVKNLRICEDC
Sbjct: 488 CKEIGYAPVSEEVMHQVEEEEKEFALRYHSEKLAVAFGLLKT-VDVAITIVKNLRICEDC 547

Query: 580 HSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           HSA K++SI+++R+I VRDK RFHHF++G+CSCLDYW
Sbjct: 548 HSAFKYISIVSNRQITVRDKLRFHHFQNGSCSCLDYW 583

BLAST of Lsi01G003140 vs. ExPASy Swiss-Prot
Match: Q9SUH6 (Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana OX=3702 GN=DYW9 PE=2 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 6.0e-133
Identity = 231/579 (39.90%), Postives = 359/579 (62.00%), Query Frame = 0

Query: 38  RLHSPVYEALLQAGPRLRNLQ---QVHAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARR 97
           RL +     +L A   L+ L+   Q+H+    +G      +LT  +SL    G I     
Sbjct: 218 RLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSA 277

Query: 98  LFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSA 157
           LF     PD   +++++   +  G +  ++  ++ ++ SG    + T  S++     L  
Sbjct: 278 LFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLML 337

Query: 158 LRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISG 217
           +     IH + +   + S   V  AL  +Y+K ++++ A+K+FD  PE+++ +WN++ISG
Sbjct: 338 I---YAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISG 397

Query: 218 YEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLN 277
           Y QNG  +++I +F  M +S F P+P TI  +LS+C+QLGAL  G W+HD      F+ +
Sbjct: 398 YTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESS 457

Query: 278 VVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRA 337
           + + T+LI MY +CG++++AR +FD M ++N VTW  MISGYG+HG G++A+ +F EM  
Sbjct: 458 IYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLN 517

Query: 338 FGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLN 397
            G  P  +TF+ VL AC+H+GL+ +G  +F+S+   YG  P V+H+ CMVD+ GRAG L 
Sbjct: 518 SGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQ 577

Query: 398 DAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIY 457
            A QFI+  +  EPG +VW ++LGACR+HK+ +L   V+E +  ++P+N G++V+LSNI+
Sbjct: 578 RALQFIEA-MSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELDPDNVGYHVLLSNIH 637

Query: 458 ALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELM 517
           +      +   VR    +R+L K  GY+ IEI    ++F+ GD+SHPQ   IY  L++L 
Sbjct: 638 SADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSHPQVKEIYEKLEKLE 697

Query: 518 GRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICE 577
           G+  E+GY P  E  +HD+E+EER+  ++ HSE+LA+AFGL+ T  G  IRI+KNLR+C 
Sbjct: 698 GKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEPGTEIRIIKNLRVCL 757

Query: 578 DCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           DCH+  K +S I +R I+VRD  RFHHFKDG CSC DYW
Sbjct: 758 DCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792

BLAST of Lsi01G003140 vs. ExPASy Swiss-Prot
Match: Q9ZVF4 (Pentatricopeptide repeat-containing protein At2g01510, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H37 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 8.7e-132
Identity = 234/591 (39.59%), Postives = 371/591 (62.77%), Query Frame = 0

Query: 25  LAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVC 84
           LA Q P+T+         + E L  +  + + L+++HA ++ +G     SLLT+LL  + 
Sbjct: 2   LAKQTPLTK-------QMLSELLRASSSKPKQLKKIHAIVLRTGFSEKNSLLTQLLENLV 61

Query: 85  AAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTS 144
             G + YAR++F  +  P  FL+++L K   +     +++L Y+ M   GV    +T+  
Sbjct: 62  VIGDMCYARQVFDEMHKPRIFLWNTLFKGYVRNQLPFESLLLYKKMRDLGVRPDEFTYPF 121

Query: 145 VIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERT 204
           V+KA + L     G  +H+HV+  G+G    V   LV +Y K  ++  A+ +F+ M  + 
Sbjct: 122 VVKAISQLGDFSCGFALHAHVVKYGFGCLGIVATELVMMYMKFGELSSAEFLFESMQVKD 181

Query: 205 IIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHD 264
           ++AWN+ ++   Q G    ++  F+ M     Q D  T+VS+LS+C QLG+L+ G  ++D
Sbjct: 182 LVAWNAFLAVCVQTGNSAIALEYFNKMCADAVQFDSFTVVSMLSACGQLGSLEIGEEIYD 241

Query: 265 YANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQ 324
            A  +  D N+++  + ++M+ +CGN   AR +F+ MK+RNVV+W+ MI GY M+G   +
Sbjct: 242 RARKEEIDCNIIVENARLDMHLKCGNTEAARVLFEEMKQRNVVSWSTMIVGYAMNGDSRE 301

Query: 325 AMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEA--YGLVPGVEHHVC 384
           A+ LF  M+  G RPN +TF+ VLSAC+H+GL+++G+  FS + ++    L P  EH+ C
Sbjct: 302 ALTLFTTMQNEGLRPNYVTFLGVLSACSHAGLVNEGKRYFSLMVQSNDKNLEPRKEHYAC 361

Query: 385 MVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPE 444
           MVD+ GR+GLL +AY+FIKK +P EP   +W ++LGAC +H++  LG KVA+ ++   P+
Sbjct: 362 MVDLLGRSGLLEEAYEFIKK-MPVEPDTGIWGALLGACAVHRDMILGQKVADVLVETAPD 421

Query: 445 NPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQ 504
              ++V+LSNIYA AG+ D V+ VR+ M +   KK   YS++E + K + F+ GDKSHPQ
Sbjct: 422 IGSYHVLLSNIYAAAGKWDCVDKVRSKMRKLGTKKVAAYSSVEFEGKIHFFNRGDKSHPQ 481

Query: 505 TNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGD 564
           +  IY  LDE++ +  + GYVP   S+ HD+E EE++ +L +HSEKLA+AFGL++   G 
Sbjct: 482 SKAIYEKLDEILKKIRKMGYVPDTCSVFHDVEMEEKECSLSHHSEKLAIAFGLIKGRPGH 541

Query: 565 TIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
            IR++KNLR C+DCH+  K VS +   EII+RDK RFHHF++G CSC ++W
Sbjct: 542 PIRVMKNLRTCDDCHAFSKFVSSLTSTEIIMRDKNRFHHFRNGVCSCKEFW 584

BLAST of Lsi01G003140 vs. ExPASy Swiss-Prot
Match: A8MQA3 (Pentatricopeptide repeat-containing protein At4g21065 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H28 PE=2 SV=2)

HSP 1 Score: 471.5 bits (1212), Expect = 1.5e-131
Identity = 236/568 (41.55%), Postives = 366/568 (64.44%), Query Frame = 0

Query: 54  LRNLQQVHAHII-----VSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTVPNP-DSFLF 113
           +  L+Q+HA  I     +S  +  + L+  L+SL  +   ++YA ++F  +  P + F++
Sbjct: 30  ITKLRQIHAFSIRHGVSISDAELGKHLIFYLVSLP-SPPPMSYAHKVFSKIEKPINVFIW 89

Query: 114 DSLLKATSKFGFSIDAVLFYRHMLFSG-VPRSNYTFTSVIKACADLSALRLGREIHSHVM 173
           ++L++  ++ G SI A   YR M  SG V    +T+  +IKA   ++ +RLG  IHS V+
Sbjct: 90  NTLIRGYAEIGNSISAFSLYREMRVSGLVEPDTHTYPFLIKAVTTMADVRLGETIHSVVI 149

Query: 174 VCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIG 233
             G+GS +YVQ +L+ LYA   D+  A KVFD MPE+ ++AWNS+I+G+ +NG P+E++ 
Sbjct: 150 RSGFGSLIYVQNSLLHLYANCGDVASAYKVFDKMPEKDLVAWNSVINGFAENGKPEEALA 209

Query: 234 VFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYT 293
           ++  M   G +PD  TIVSLLS+C+++GAL  G  +H Y    G   N+     L+++Y 
Sbjct: 210 LYTEMNSKGIKPDGFTIVSLLSACAKIGALTLGKRVHVYMIKVGLTRNLHSSNVLLDLYA 269

Query: 294 RCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAF-GPRPNNITFV 353
           RCG V +A+ +FD M ++N V+WT++I G  ++G+G++A++LF  M +  G  P  ITFV
Sbjct: 270 RCGRVEEAKTLFDEMVDKNSVSWTSLIVGLAVNGFGKEAIELFKYMESTEGLLPCEITFV 329

Query: 354 AVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVP 413
            +L AC+H G++ +G   F  ++E Y + P +EH  CMVD+  RAG +  AY++IK  +P
Sbjct: 330 GILYACSHCGMVKEGFEYFRRMREEYKIEPRIEHFGCMVDLLARAGQVKKAYEYIKS-MP 389

Query: 414 KEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEM 473
            +P   +W ++LGAC +H + DL       +L +EP + G YV+LSN+YA   R   V+ 
Sbjct: 390 MQPNVVIWRTLLGACTVHGDSDLAEFARIQILQLEPNHSGDYVLLSNMYASEQRWSDVQK 449

Query: 474 VRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPA 533
           +R  M+R  +KK  G+S +E+  + + F MGDKSHPQ++ IY  L E+ GR    GYVP 
Sbjct: 450 IRKQMLRDGVKKVPGHSLVEVGNRVHEFLMGDKSHPQSDAIYAKLKEMTGRLRSEGYVPQ 509

Query: 534 LESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSI 593
           + ++  D+E+EE++ A+ YHSEK+A+AF L+ T +   I +VKNLR+C DCH AIK VS 
Sbjct: 510 ISNVYVDVEEEEKENAVVYHSEKIAIAFMLISTPERSPITVVKNLRVCADCHLAIKLVSK 569

Query: 594 IADREIIVRDKFRFHHFKDGACSCLDYW 614
           + +REI+VRD+ RFHHFK+G+CSC DYW
Sbjct: 570 VYNREIVVRDRSRFHHFKNGSCSCQDYW 595

BLAST of Lsi01G003140 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.5e-131
Identity = 236/612 (38.56%), Postives = 376/612 (61.44%), Query Frame = 0

Query: 39  LHSPVYEAL--LQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVCAAGS---ITYAR 98
           L   +YE +  LQ   +   L+Q+HA ++ +GL +    +TK LS   ++ S   + YA+
Sbjct: 10  LEHNLYETMSCLQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQ 69

Query: 99  RLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLS 158
            +F     PD+FL++ +++  S       ++L Y+ ML S  P + YTF S++KAC++LS
Sbjct: 70  IVFDGFDRPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLS 129

Query: 159 ALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNS--- 218
           A     +IH+ +   GY +D+Y   +L+  YA   + K+A  +FD +PE   ++WNS   
Sbjct: 130 AFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIK 189

Query: 219 ----------------------------LISGYEQNGFPKESIGVFHLMLESGFQPDPTT 278
                                       +ISGY Q    KE++ +FH M  S  +PD  +
Sbjct: 190 GYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVS 249

Query: 279 IVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMK 338
           + + LS+C+QLGAL+ G W+H Y N     ++ VLG  LI+MY +CG + +A EVF ++K
Sbjct: 250 LANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIK 309

Query: 339 ERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRG 398
           +++V  WTA+ISGY  HG+G +A+  F EM+  G +PN ITF AVL+AC+++GL+++G+ 
Sbjct: 310 KKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKL 369

Query: 399 VFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRM 458
           +F S++  Y L P +EH+ C+VD+ GRAGLL++A +FI++ +P +P   +W ++L ACR+
Sbjct: 370 IFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQE-MPLKPNAVIWGALLKACRI 429

Query: 459 HKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYS 518
           HKN +LG ++ E ++A++P + G YV  +NI+A+  + D+    R +M  + + K  G S
Sbjct: 430 HKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCS 489

Query: 519 TIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDL-EDEERDYA 578
           TI ++  T+ F  GD+SHP+   I      +  +  E+GYVP LE ++ DL +D+ER+  
Sbjct: 490 TISLEGTTHEFLAGDRSHPEIEKIQSKWRIMRRKLEENGYVPELEEMLLDLVDDDEREAI 549

Query: 579 LRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHH 614
           +  HSEKLA+ +GL++T  G  IRI+KNLR+C+DCH   K +S I  R+I++RD+ RFHH
Sbjct: 550 VHQHSEKLAITYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHH 609

BLAST of Lsi01G003140 vs. ExPASy TrEMBL
Match: A0A5D3CPN1 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007480 PE=3 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 546/599 (91.15%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 15  MNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRS 74
           MNKI L+T+QLAFQHPVTR FD +  SPVYEALL++GPRLRNLQQVHAHIIVSGL RSRS
Sbjct: 1   MNKIKLQTQQLAFQHPVTRNFDTQSRSPVYEALLRSGPRLRNLQQVHAHIIVSGLHRSRS 60

Query: 75  LLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSG 134
           LLTKL+SLVC AGSITYARRLFPTVPNPDSFLFDSLLK TSKFGFSIDAVLFYRHMLFSG
Sbjct: 61  LLTKLISLVCTAGSITYARRLFPTVPNPDSFLFDSLLKVTSKFGFSIDAVLFYRHMLFSG 120

Query: 135 VPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAK 194
            P SNYTFTSVIKACADLSALRLG+EIHSHVMVCGYGSDMYVQAAL+ALYAKASDMKVAK
Sbjct: 121 APPSNYTFTSVIKACADLSALRLGKEIHSHVMVCGYGSDMYVQAALIALYAKASDMKVAK 180

Query: 195 KVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLG 254
           KVFD MP+RTIIAWNSLISG+EQNG P+ESI +FHLM++SG QPDP TIVSLLSSCSQLG
Sbjct: 181 KVFDAMPQRTIIAWNSLISGFEQNGLPQESIDLFHLMMKSGLQPDPATIVSLLSSCSQLG 240

Query: 255 ALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 314
           ALDFGCWLHDY+N  GFDLNVVLGTSLINMYTRCGNVSKA+EVFDSMKERNVVTWTAMIS
Sbjct: 241 ALDFGCWLHDYSNVNGFDLNVVLGTSLINMYTRCGNVSKAQEVFDSMKERNVVTWTAMIS 300

Query: 315 GYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLV 374
           GYGMHG+G+QAMKLFGEMRA+GPRPNNITFVAVLSACAHSGLIDDGR VFSS+KE YGLV
Sbjct: 301 GYGMHGHGKQAMKLFGEMRAYGPRPNNITFVAVLSACAHSGLIDDGRRVFSSMKEVYGLV 360

Query: 375 PGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAE 434
           PGVEH+VCMVDMFGRAGLLNDAYQFIKK +PKEPGPAVWTSMLGACRMH+NFDLGVKVAE
Sbjct: 361 PGVEHNVCMVDMFGRAGLLNDAYQFIKKILPKEPGPAVWTSMLGACRMHRNFDLGVKVAE 420

Query: 435 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFS 494
           HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMM RRRLKKQVGYSTIEI RKTYLFS
Sbjct: 421 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMTRRRLKKQVGYSTIEINRKTYLFS 480

Query: 495 MGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFG 554
           MGDKSHPQTNTIYRYLDELM RCSESGYVPA ESLMHDLE+EERDYALRYHSEKLALAFG
Sbjct: 481 MGDKSHPQTNTIYRYLDELMCRCSESGYVPAPESLMHDLEEEERDYALRYHSEKLALAFG 540

Query: 555 LLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           LL TNQG+TIRIVKNLR+CEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLDYW
Sbjct: 541 LLNTNQGETIRIVKNLRMCEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLDYW 599

BLAST of Lsi01G003140 vs. ExPASy TrEMBL
Match: A0A1S4DTH4 (pentatricopeptide repeat-containing protein At2g33760 OS=Cucumis melo OX=3656 GN=LOC103484519 PE=3 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 546/599 (91.15%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 15  MNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRS 74
           MNKI L+T+QLAFQHPVTR FD +  SPVYEALL++GPRLRNLQQVHAHIIVSGL RSRS
Sbjct: 1   MNKIKLQTQQLAFQHPVTRNFDTQSRSPVYEALLRSGPRLRNLQQVHAHIIVSGLHRSRS 60

Query: 75  LLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSG 134
           LLTKL+SLVC AGSITYARRLFPTVPNPDSFLFDSLLK TSKFGFSIDAVLFYRHMLFSG
Sbjct: 61  LLTKLISLVCTAGSITYARRLFPTVPNPDSFLFDSLLKVTSKFGFSIDAVLFYRHMLFSG 120

Query: 135 VPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAK 194
            P SNYTFTSVIKACADLSALRLG+EIHSHVMVCGYGSDMYVQAAL+ALYAKASDMKVAK
Sbjct: 121 APPSNYTFTSVIKACADLSALRLGKEIHSHVMVCGYGSDMYVQAALIALYAKASDMKVAK 180

Query: 195 KVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLG 254
           KVFD MP+RTIIAWNSLISG+EQNG P+ESI +FHLM++SG QPDP TIVSLLSSCSQLG
Sbjct: 181 KVFDAMPQRTIIAWNSLISGFEQNGLPQESIDLFHLMMKSGLQPDPATIVSLLSSCSQLG 240

Query: 255 ALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 314
           ALDFGCWLHDY+N  GFDLNVVLGTSLINMYTRCGNVSKA+EVFDSMKERNVVTWTAMIS
Sbjct: 241 ALDFGCWLHDYSNVNGFDLNVVLGTSLINMYTRCGNVSKAQEVFDSMKERNVVTWTAMIS 300

Query: 315 GYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLV 374
           GYGMHG+G+QAMKLFGEMRA+GPRPNNITFVAVLSACAHSGLIDDGR VFSS+KE YGLV
Sbjct: 301 GYGMHGHGKQAMKLFGEMRAYGPRPNNITFVAVLSACAHSGLIDDGRRVFSSMKEVYGLV 360

Query: 375 PGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAE 434
           PGVEH+VCMVDMFGRAGLLNDAYQFIKK +PKEPGPAVWTSMLGACRMH+NFDLGVKVAE
Sbjct: 361 PGVEHNVCMVDMFGRAGLLNDAYQFIKKILPKEPGPAVWTSMLGACRMHRNFDLGVKVAE 420

Query: 435 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFS 494
           HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMM RRRLKKQVGYSTIEI RKTYLFS
Sbjct: 421 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMTRRRLKKQVGYSTIEINRKTYLFS 480

Query: 495 MGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFG 554
           MGDKSHPQTNTIYRYLDELM RCSESGYVPA ESLMHDLE+EERDYALRYHSEKLALAFG
Sbjct: 481 MGDKSHPQTNTIYRYLDELMCRCSESGYVPAPESLMHDLEEEERDYALRYHSEKLALAFG 540

Query: 555 LLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           LL TNQG+TIRIVKNLR+CEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLDYW
Sbjct: 541 LLNTNQGETIRIVKNLRMCEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLDYW 599

BLAST of Lsi01G003140 vs. ExPASy TrEMBL
Match: A0A6J1EL35 (pentatricopeptide repeat-containing protein At2g33760 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433589 PE=3 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 531/603 (88.06%), Postives = 568/603 (94.20%), Query Frame = 0

Query: 11  INPIMNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQ 70
           +N   + I +E++QLA QHPVT IFD+R HSP+Y ALLQAGPRLRNLQQVHAHIIVSG  
Sbjct: 1   MNKTKSLITVESQQLALQHPVTHIFDSRPHSPIYAALLQAGPRLRNLQQVHAHIIVSGFH 60

Query: 71  RSRSLLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHM 130
           RSRSLLTKLLSL+CAAGSITYAR+L PTVPNPDSFLF+SLLKATSK+GFS+DAVLFYRHM
Sbjct: 61  RSRSLLTKLLSLICAAGSITYARQLLPTVPNPDSFLFNSLLKATSKYGFSVDAVLFYRHM 120

Query: 131 LFSGVPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDM 190
           LF GV +SNYTFTSVIKACADLSALRLGREIHSHV+V GYGSDMYVQAAL+ALYAKA DM
Sbjct: 121 LFLGVSQSNYTFTSVIKACADLSALRLGREIHSHVVVSGYGSDMYVQAALIALYAKAGDM 180

Query: 191 KVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSC 250
           KVA+KVFD MP+RT IAWNSLISGYEQNG P +SI +F+LM+ SGFQPD  T+VSLLSSC
Sbjct: 181 KVAQKVFDEMPQRTTIAWNSLISGYEQNGLPGKSIDLFNLMIGSGFQPDSATVVSLLSSC 240

Query: 251 SQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWT 310
           SQLGALDFGCWLHDYAN   FDLNVVLGTSLINMYTRCGNVSKAREVFDSMK RNVVTWT
Sbjct: 241 SQLGALDFGCWLHDYANSNSFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKVRNVVTWT 300

Query: 311 AMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEA 370
           AMISGYGMHGYGEQAMKLF EMRA+GPRPNNITFVAVLSACAHSGLIDDGR  FSS+KE 
Sbjct: 301 AMISGYGMHGYGEQAMKLFNEMRAYGPRPNNITFVAVLSACAHSGLIDDGRRAFSSMKEV 360

Query: 371 YGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGV 430
           YGLVPGVEHHVCMVDMFGRAGLL DAYQFI+KF+P+EPGPAVWTSMLGACRMHKNFDLGV
Sbjct: 361 YGLVPGVEHHVCMVDMFGRAGLLKDAYQFIRKFLPEEPGPAVWTSMLGACRMHKNFDLGV 420

Query: 431 KVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKT 490
           KVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMM+RRRLKKQVGYSTIEI +KT
Sbjct: 421 KVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMVRRRLKKQVGYSTIEINQKT 480

Query: 491 YLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLA 550
           Y+FSMGDKSHP+TN IYRYLDELMGRCSESGY+PALESLMHDLE+EERDYALRYHSEKLA
Sbjct: 481 YMFSMGDKSHPETNAIYRYLDELMGRCSESGYIPALESLMHDLEEEERDYALRYHSEKLA 540

Query: 551 LAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCL 610
           LAFGLL+TNQG+TIRIVKNLRICEDCHSAIKH+S+IADREIIVRDKFRFHHFKDGACSCL
Sbjct: 541 LAFGLLKTNQGETIRIVKNLRICEDCHSAIKHISVIADREIIVRDKFRFHHFKDGACSCL 600

Query: 611 DYW 614
           DYW
Sbjct: 601 DYW 603

BLAST of Lsi01G003140 vs. ExPASy TrEMBL
Match: A0A6J1KND5 (pentatricopeptide repeat-containing protein At2g33760 OS=Cucurbita maxima OX=3661 GN=LOC111496866 PE=3 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 529/597 (88.61%), Postives = 564/597 (94.47%), Query Frame = 0

Query: 17  KIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLL 76
           K  LE++QLA QHPVT IFD+R HSP+Y ALLQAGPRLRNLQQVHAHIIVSG  RSRSLL
Sbjct: 29  KCKLESQQLALQHPVTHIFDSRPHSPIYAALLQAGPRLRNLQQVHAHIIVSGFHRSRSLL 88

Query: 77  TKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVP 136
           TKLLSLVCAAGSITYARRL PTVPNPDSFLF+SLLKATSK+GFS+DAVLFYRHMLF GV 
Sbjct: 89  TKLLSLVCAAGSITYARRLLPTVPNPDSFLFNSLLKATSKYGFSVDAVLFYRHMLFLGVS 148

Query: 137 RSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKV 196
           +SNYTFTSVIKACADLSALRLGREIHSHV+V GYGSDMYVQAAL+ALYAKA DMKVA+KV
Sbjct: 149 QSNYTFTSVIKACADLSALRLGREIHSHVVVSGYGSDMYVQAALIALYAKAGDMKVAQKV 208

Query: 197 FDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGAL 256
           FD MP+RT IAWNSLISGYEQNG P +SI +F+ M+ SGFQPD  T+VSLLSSCSQLGAL
Sbjct: 209 FDEMPQRTTIAWNSLISGYEQNGLPAKSIDLFNPMIGSGFQPDSATVVSLLSSCSQLGAL 268

Query: 257 DFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGY 316
           DFGCWLHDYAN   F+LNVVLGTSLINMYTRCGNVSKAREVFDSMK RNVVTWTAMISGY
Sbjct: 269 DFGCWLHDYANSNSFNLNVVLGTSLINMYTRCGNVSKAREVFDSMKVRNVVTWTAMISGY 328

Query: 317 GMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPG 376
           GMHGYGEQAMKLF EMRA+GPRPNNITFVAVLSACAHSGLIDDGR  FSS+KE YGLVPG
Sbjct: 329 GMHGYGEQAMKLFNEMRAYGPRPNNITFVAVLSACAHSGLIDDGRQAFSSMKEVYGLVPG 388

Query: 377 VEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHV 436
           VEHHVCMVDMFGRAGLL DAYQFI+KF+P+EPGPAVWTSMLGACRMHKNFDLGVKVAEHV
Sbjct: 389 VEHHVCMVDMFGRAGLLKDAYQFIRKFLPEEPGPAVWTSMLGACRMHKNFDLGVKVAEHV 448

Query: 437 LAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMG 496
           LAVEPENPGHYVMLSNIYALAGRMD+VEMVRNMM+RRRLKKQVGYSTIEI +KTY+FSMG
Sbjct: 449 LAVEPENPGHYVMLSNIYALAGRMDQVEMVRNMMVRRRLKKQVGYSTIEINQKTYMFSMG 508

Query: 497 DKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLL 556
           DKSHP+TN IYRYLDELMGRCSESGY+PALESLMHDLE+EERDYALRYHSE+LALAFGLL
Sbjct: 509 DKSHPETNAIYRYLDELMGRCSESGYIPALESLMHDLEEEERDYALRYHSERLALAFGLL 568

Query: 557 ETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           +TNQG+TIRIVKNLRICEDCHSAIKH+S+IADREIIVRDKFRFHHFKDGACSCLDYW
Sbjct: 569 KTNQGETIRIVKNLRICEDCHSAIKHISVIADREIIVRDKFRFHHFKDGACSCLDYW 625

BLAST of Lsi01G003140 vs. ExPASy TrEMBL
Match: A0A0A0KK64 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517370 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 530/602 (88.04%), Postives = 556/602 (92.36%), Query Frame = 0

Query: 12  NPIMNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQR 71
           NP MNKI L+T+QLAFQHPVTR FD + HSPV+EALL++GPRLRNLQQVHAHIIVSGL R
Sbjct: 62  NPTMNKIKLQTQQLAFQHPVTRNFDTQSHSPVHEALLRSGPRLRNLQQVHAHIIVSGLHR 121

Query: 72  SRSLLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHML 131
           SRSLLTKL+SLVC AGSITYARRLFPTVPNPDSFLFDSLLK TSKFGFSID VLFYR ML
Sbjct: 122 SRSLLTKLISLVCTAGSITYARRLFPTVPNPDSFLFDSLLKVTSKFGFSIDTVLFYRRML 181

Query: 132 FSGVPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMK 191
           FSG P+SNYTFTSVIKACADLSALRLG+EIHSHVMVCGYGSDMYVQAAL+ALYAKASDMK
Sbjct: 182 FSGAPQSNYTFTSVIKACADLSALRLGKEIHSHVMVCGYGSDMYVQAALIALYAKASDMK 241

Query: 192 VAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCS 251
           VAKKVFD MP+RTIIAWNSLISGY+QNG P+ESIG+FHLM+ESGFQPD  TIVSLLSSCS
Sbjct: 242 VAKKVFDAMPQRTIIAWNSLISGYDQNGLPQESIGLFHLMMESGFQPDSATIVSLLSSCS 301

Query: 252 QLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTA 311
           QLGALDFGCWLHDYA+G GFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTA
Sbjct: 302 QLGALDFGCWLHDYADGNGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTA 361

Query: 312 MISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAY 371
           MISGYGMHGY                         VLSACAHSGLIDDGR VFSS+KEAY
Sbjct: 362 MISGYGMHGY-------------------------VLSACAHSGLIDDGRRVFSSMKEAY 421

Query: 372 GLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVK 431
           GLVPGVEH+VCMVDMFGRAGLLNDAYQFIKKF+PKEPGPAVWTSMLGACRMH+NFDLGVK
Sbjct: 422 GLVPGVEHNVCMVDMFGRAGLLNDAYQFIKKFIPKEPGPAVWTSMLGACRMHRNFDLGVK 481

Query: 432 VAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTY 491
           VAEHVL+VEPENPGHYVMLSNIYALAGRMDRVEMVRNMM RRRLKKQVGYSTIEI RKTY
Sbjct: 482 VAEHVLSVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMTRRRLKKQVGYSTIEINRKTY 541

Query: 492 LFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLAL 551
           LFSMGDKSHPQTNTIYRYLDELM RCSESGYVPA ESLMHDLE+EERDYALRYHSEKLAL
Sbjct: 542 LFSMGDKSHPQTNTIYRYLDELMCRCSESGYVPAPESLMHDLEEEERDYALRYHSEKLAL 601

Query: 552 AFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLD 611
           AFGLL+TNQG+TIRIVKNLRICEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLD
Sbjct: 602 AFGLLKTNQGETIRIVKNLRICEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLD 638

Query: 612 YW 614
           YW
Sbjct: 662 YW 638

BLAST of Lsi01G003140 vs. NCBI nr
Match: XP_038881818.1 (pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881819.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881820.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881821.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881822.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881823.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881824.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881825.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881826.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881827.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881828.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881829.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881830.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881831.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881832.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881833.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881834.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881835.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881836.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881837.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881838.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida] >XP_038881839.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hispida])

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 578/613 (94.29%), Postives = 595/613 (97.06%), Query Frame = 0

Query: 1   MQKKIHLPEIINPIMNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQV 60
           +QKKIHLPEIINP MNKI LETEQL FQHPVTRIF +R HSPVY+ALLQAG RLR+LQQV
Sbjct: 91  VQKKIHLPEIINPTMNKIKLETEQLPFQHPVTRIFYSRSHSPVYKALLQAGSRLRSLQQV 150

Query: 61  HAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFS 120
           HAHIIVSGL  SRSLLTKL+SLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKF FS
Sbjct: 151 HAHIIVSGLHTSRSLLTKLISLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFAFS 210

Query: 121 IDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL 180
           IDAVLFYRHMLFSGVP+SNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL
Sbjct: 211 IDAVLFYRHMLFSGVPQSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL 270

Query: 181 VALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDP 240
           +ALYAKASDMKVAKKVFD MP+RTIIAWNSLISGYEQNGFPKESIG+FHLMLESGFQPDP
Sbjct: 271 IALYAKASDMKVAKKVFDAMPQRTIIAWNSLISGYEQNGFPKESIGIFHLMLESGFQPDP 330

Query: 241 TTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDS 300
           TTIVSLLSSCSQLGALDFGCWLHDYANG GFDLNVVLGTSLINMYTRCGNVSKAREVFDS
Sbjct: 331 TTIVSLLSSCSQLGALDFGCWLHDYANGNGFDLNVVLGTSLINMYTRCGNVSKAREVFDS 390

Query: 301 MKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDG 360
           MKERNVVTWTAMISGYGMHG GEQAMKLFGEMRA GPRPNNITFVAVLSACAHSGLIDDG
Sbjct: 391 MKERNVVTWTAMISGYGMHGNGEQAMKLFGEMRAHGPRPNNITFVAVLSACAHSGLIDDG 450

Query: 361 RGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGAC 420
           R VFSS+KEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIK F+PKEPG AVWTSMLGAC
Sbjct: 451 RRVFSSMKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKNFLPKEPGAAVWTSMLGAC 510

Query: 421 RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG 480
           RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG
Sbjct: 511 RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG 570

Query: 481 YSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDY 540
           YS+IEI RKTYLFSMGDKSHPQT+TIYRYLDELMGRCSESGYVPA ESLMHDLEDEERDY
Sbjct: 571 YSSIEINRKTYLFSMGDKSHPQTSTIYRYLDELMGRCSESGYVPAPESLMHDLEDEERDY 630

Query: 541 ALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFH 600
           ALRYHSEK+ALAFGLLETNQG+TIRIVKNLRICEDCHSAIKH+SIIADREIIVRDKFRFH
Sbjct: 631 ALRYHSEKVALAFGLLETNQGETIRIVKNLRICEDCHSAIKHISIIADREIIVRDKFRFH 690

Query: 601 HFKDGACSCLDYW 614
           HFKDG+CSCLDYW
Sbjct: 691 HFKDGSCSCLDYW 703

BLAST of Lsi01G003140 vs. NCBI nr
Match: XP_038881841.1 (pentatricopeptide repeat-containing protein At2g33760 isoform X3 [Benincasa hispida])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 566/599 (94.49%), Postives = 582/599 (97.16%), Query Frame = 0

Query: 15  MNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRS 74
           MNKI LETEQL FQHPVTRIF +R HSPVY+ALLQAG RLR+LQQVHAHIIVSGL  SRS
Sbjct: 1   MNKIKLETEQLPFQHPVTRIFYSRSHSPVYKALLQAGSRLRSLQQVHAHIIVSGLHTSRS 60

Query: 75  LLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSG 134
           LLTKL+SLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKF FSIDAVLFYRHMLFSG
Sbjct: 61  LLTKLISLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFAFSIDAVLFYRHMLFSG 120

Query: 135 VPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAK 194
           VP+SNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL+ALYAKASDMKVAK
Sbjct: 121 VPQSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALIALYAKASDMKVAK 180

Query: 195 KVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLG 254
           KVFD MP+RTIIAWNSLISGYEQNGFPKESIG+FHLMLESGFQPDPTTIVSLLSSCSQLG
Sbjct: 181 KVFDAMPQRTIIAWNSLISGYEQNGFPKESIGIFHLMLESGFQPDPTTIVSLLSSCSQLG 240

Query: 255 ALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 314
           ALDFGCWLHDYANG GFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS
Sbjct: 241 ALDFGCWLHDYANGNGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 300

Query: 315 GYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLV 374
           GYGMHG GEQAMKLFGEMRA GPRPNNITFVAVLSACAHSGLIDDGR VFSS+KEAYGLV
Sbjct: 301 GYGMHGNGEQAMKLFGEMRAHGPRPNNITFVAVLSACAHSGLIDDGRRVFSSMKEAYGLV 360

Query: 375 PGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAE 434
           PGVEHHVCMVDMFGRAGLLNDAYQFIK F+PKEPG AVWTSMLGACRMHKNFDLGVKVAE
Sbjct: 361 PGVEHHVCMVDMFGRAGLLNDAYQFIKNFLPKEPGAAVWTSMLGACRMHKNFDLGVKVAE 420

Query: 435 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFS 494
           HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYS+IEI RKTYLFS
Sbjct: 421 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSSIEINRKTYLFS 480

Query: 495 MGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFG 554
           MGDKSHPQT+TIYRYLDELMGRCSESGYVPA ESLMHDLEDEERDYALRYHSEK+ALAFG
Sbjct: 481 MGDKSHPQTSTIYRYLDELMGRCSESGYVPAPESLMHDLEDEERDYALRYHSEKVALAFG 540

Query: 555 LLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           LLETNQG+TIRIVKNLRICEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLDYW
Sbjct: 541 LLETNQGETIRIVKNLRICEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLDYW 599

BLAST of Lsi01G003140 vs. NCBI nr
Match: XP_004134955.1 (pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus] >XP_031742767.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus] >XP_031742768.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus] >XP_031742769.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus] >XP_031742770.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus] >XP_031742771.1 pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativus])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 549/599 (91.65%), Postives = 577/599 (96.33%), Query Frame = 0

Query: 15  MNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRS 74
           MNKI L+T+QLAFQHPVTR FD + HSPV+EALL++GPRLRNLQQVHAHIIVSGL RSRS
Sbjct: 1   MNKIKLQTQQLAFQHPVTRNFDTQSHSPVHEALLRSGPRLRNLQQVHAHIIVSGLHRSRS 60

Query: 75  LLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSG 134
           LLTKL+SLVC AGSITYARRLFPTVPNPDSFLFDSLLK TSKFGFSID VLFYR MLFSG
Sbjct: 61  LLTKLISLVCTAGSITYARRLFPTVPNPDSFLFDSLLKVTSKFGFSIDTVLFYRRMLFSG 120

Query: 135 VPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAK 194
            P+SNYTFTSVIKACADLSALRLG+EIHSHVMVCGYGSDMYVQAAL+ALYAKASDMKVAK
Sbjct: 121 APQSNYTFTSVIKACADLSALRLGKEIHSHVMVCGYGSDMYVQAALIALYAKASDMKVAK 180

Query: 195 KVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLG 254
           KVFD MP+RTIIAWNSLISGY+QNG P+ESIG+FHLM+ESGFQPD  TIVSLLSSCSQLG
Sbjct: 181 KVFDAMPQRTIIAWNSLISGYDQNGLPQESIGLFHLMMESGFQPDSATIVSLLSSCSQLG 240

Query: 255 ALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 314
           ALDFGCWLHDYA+G GFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS
Sbjct: 241 ALDFGCWLHDYADGNGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 300

Query: 315 GYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLV 374
           GYGMHGYG QAM+LF EMRA+GPRPNNITFVAVLSACAHSGLIDDGR VFSS+KEAYGLV
Sbjct: 301 GYGMHGYGRQAMELFTEMRAYGPRPNNITFVAVLSACAHSGLIDDGRRVFSSMKEAYGLV 360

Query: 375 PGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAE 434
           PGVEH+VCMVDMFGRAGLLNDAYQFIKKF+PKEPGPAVWTSMLGACRMH+NFDLGVKVAE
Sbjct: 361 PGVEHNVCMVDMFGRAGLLNDAYQFIKKFIPKEPGPAVWTSMLGACRMHRNFDLGVKVAE 420

Query: 435 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFS 494
           HVL+VEPENPGHYVMLSNIYALAGRMDRVEMVRNMM RRRLKKQVGYSTIEI RKTYLFS
Sbjct: 421 HVLSVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMTRRRLKKQVGYSTIEINRKTYLFS 480

Query: 495 MGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFG 554
           MGDKSHPQTNTIYRYLDELM RCSESGYVPA ESLMHDLE+EERDYALRYHSEKLALAFG
Sbjct: 481 MGDKSHPQTNTIYRYLDELMCRCSESGYVPAPESLMHDLEEEERDYALRYHSEKLALAFG 540

Query: 555 LLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           LL+TNQG+TIRIVKNLRICEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLDYW
Sbjct: 541 LLKTNQGETIRIVKNLRICEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLDYW 599

BLAST of Lsi01G003140 vs. NCBI nr
Match: XP_016899291.1 (PREDICTED: pentatricopeptide repeat-containing protein At2g33760 [Cucumis melo] >KAA0052686.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK13138.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 546/599 (91.15%), Postives = 574/599 (95.83%), Query Frame = 0

Query: 15  MNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRS 74
           MNKI L+T+QLAFQHPVTR FD +  SPVYEALL++GPRLRNLQQVHAHIIVSGL RSRS
Sbjct: 1   MNKIKLQTQQLAFQHPVTRNFDTQSRSPVYEALLRSGPRLRNLQQVHAHIIVSGLHRSRS 60

Query: 75  LLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSG 134
           LLTKL+SLVC AGSITYARRLFPTVPNPDSFLFDSLLK TSKFGFSIDAVLFYRHMLFSG
Sbjct: 61  LLTKLISLVCTAGSITYARRLFPTVPNPDSFLFDSLLKVTSKFGFSIDAVLFYRHMLFSG 120

Query: 135 VPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAK 194
            P SNYTFTSVIKACADLSALRLG+EIHSHVMVCGYGSDMYVQAAL+ALYAKASDMKVAK
Sbjct: 121 APPSNYTFTSVIKACADLSALRLGKEIHSHVMVCGYGSDMYVQAALIALYAKASDMKVAK 180

Query: 195 KVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLG 254
           KVFD MP+RTIIAWNSLISG+EQNG P+ESI +FHLM++SG QPDP TIVSLLSSCSQLG
Sbjct: 181 KVFDAMPQRTIIAWNSLISGFEQNGLPQESIDLFHLMMKSGLQPDPATIVSLLSSCSQLG 240

Query: 255 ALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMIS 314
           ALDFGCWLHDY+N  GFDLNVVLGTSLINMYTRCGNVSKA+EVFDSMKERNVVTWTAMIS
Sbjct: 241 ALDFGCWLHDYSNVNGFDLNVVLGTSLINMYTRCGNVSKAQEVFDSMKERNVVTWTAMIS 300

Query: 315 GYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLV 374
           GYGMHG+G+QAMKLFGEMRA+GPRPNNITFVAVLSACAHSGLIDDGR VFSS+KE YGLV
Sbjct: 301 GYGMHGHGKQAMKLFGEMRAYGPRPNNITFVAVLSACAHSGLIDDGRRVFSSMKEVYGLV 360

Query: 375 PGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAE 434
           PGVEH+VCMVDMFGRAGLLNDAYQFIKK +PKEPGPAVWTSMLGACRMH+NFDLGVKVAE
Sbjct: 361 PGVEHNVCMVDMFGRAGLLNDAYQFIKKILPKEPGPAVWTSMLGACRMHRNFDLGVKVAE 420

Query: 435 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFS 494
           HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMM RRRLKKQVGYSTIEI RKTYLFS
Sbjct: 421 HVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMTRRRLKKQVGYSTIEINRKTYLFS 480

Query: 495 MGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFG 554
           MGDKSHPQTNTIYRYLDELM RCSESGYVPA ESLMHDLE+EERDYALRYHSEKLALAFG
Sbjct: 481 MGDKSHPQTNTIYRYLDELMCRCSESGYVPAPESLMHDLEEEERDYALRYHSEKLALAFG 540

Query: 555 LLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           LL TNQG+TIRIVKNLR+CEDCHSAIKH+SIIADREIIVRDKFRFHHFKDG+CSCLDYW
Sbjct: 541 LLNTNQGETIRIVKNLRMCEDCHSAIKHISIIADREIIVRDKFRFHHFKDGSCSCLDYW 599

BLAST of Lsi01G003140 vs. NCBI nr
Match: XP_038881840.1 (pentatricopeptide repeat-containing protein At2g33760 isoform X2 [Benincasa hispida])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 554/613 (90.38%), Postives = 571/613 (93.15%), Query Frame = 0

Query: 1   MQKKIHLPEIINPIMNKIILETEQLAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQV 60
           +QKKIHLPEIINP MNKI LETEQL FQHPVTRIF +R HSPVY+ALLQAG RLR+LQQV
Sbjct: 91  VQKKIHLPEIINPTMNKIKLETEQLPFQHPVTRIFYSRSHSPVYKALLQAGSRLRSLQQV 150

Query: 61  HAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFS 120
           HAHIIVSGL  SRSLLTKL+SLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKF FS
Sbjct: 151 HAHIIVSGLHTSRSLLTKLISLVCAAGSITYARRLFPTVPNPDSFLFDSLLKATSKFAFS 210

Query: 121 IDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL 180
           IDAVLFYRHMLFSGVP+SNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL
Sbjct: 211 IDAVLFYRHMLFSGVPQSNYTFTSVIKACADLSALRLGREIHSHVMVCGYGSDMYVQAAL 270

Query: 181 VALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDP 240
           +ALYAKASDMKVAKKVFD MP+RTIIAWNSLISGYEQNGFPKESIG+FHLMLESGFQPDP
Sbjct: 271 IALYAKASDMKVAKKVFDAMPQRTIIAWNSLISGYEQNGFPKESIGIFHLMLESGFQPDP 330

Query: 241 TTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDS 300
           TTIVSLLSSCSQLGALDFGCWLHDYANG GFDLNVVLGTSLINMYTRCGNVSKAREVFDS
Sbjct: 331 TTIVSLLSSCSQLGALDFGCWLHDYANGNGFDLNVVLGTSLINMYTRCGNVSKAREVFDS 390

Query: 301 MKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDG 360
           MKERNVVTWTAMISGYGMHG                          VLSACAHSGLIDDG
Sbjct: 391 MKERNVVTWTAMISGYGMHG-------------------------NVLSACAHSGLIDDG 450

Query: 361 RGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGAC 420
           R VFSS+KEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIK F+PKEPG AVWTSMLGAC
Sbjct: 451 RRVFSSMKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKNFLPKEPGAAVWTSMLGAC 510

Query: 421 RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG 480
           RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG
Sbjct: 511 RMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVG 570

Query: 481 YSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDY 540
           YS+IEI RKTYLFSMGDKSHPQT+TIYRYLDELMGRCSESGYVPA ESLMHDLEDEERDY
Sbjct: 571 YSSIEINRKTYLFSMGDKSHPQTSTIYRYLDELMGRCSESGYVPAPESLMHDLEDEERDY 630

Query: 541 ALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFH 600
           ALRYHSEK+ALAFGLLETNQG+TIRIVKNLRICEDCHSAIKH+SIIADREIIVRDKFRFH
Sbjct: 631 ALRYHSEKVALAFGLLETNQGETIRIVKNLRICEDCHSAIKHISIIADREIIVRDKFRFH 678

Query: 601 HFKDGACSCLDYW 614
           HFKDG+CSCLDYW
Sbjct: 691 HFKDGSCSCLDYW 678

BLAST of Lsi01G003140 vs. TAIR 10
Match: AT2G33760.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 761.9 bits (1966), Expect = 3.9e-220
Identity = 353/577 (61.18%), Postives = 460/577 (79.72%), Query Frame = 0

Query: 40  HSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTV 99
           +S  YEA+++AGPR++ LQQVHAH+IV+G  RSRSLLTKL++L C+A +I Y   LF +V
Sbjct: 8   NSAAYEAIVRAGPRVKQLQQVHAHLIVTGYGRSRSLLTKLITLACSARAIAYTHLLFLSV 67

Query: 100 PNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSALRLGR 159
           P PD FLF+S++K+TSK    +  V +YR ML S V  SNYTFTSVIK+CADLSALR+G+
Sbjct: 68  PLPDDFLFNSVIKSTSKLRLPLHCVAYYRRMLSSNVSPSNYTFTSVIKSCADLSALRIGK 127

Query: 160 EIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNG 219
            +H H +V G+G D YVQAALV  Y+K  DM+ A++VFD MPE++I+AWNSL+SG+EQNG
Sbjct: 128 GVHCHAVVSGFGLDTYVQAALVTFYSKCGDMEGARQVFDRMPEKSIVAWNSLVSGFEQNG 187

Query: 220 FPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGT 279
              E+I VF+ M ESGF+PD  T VSLLS+C+Q GA+  G W+H Y   +G DLNV LGT
Sbjct: 188 LADEAIQVFYQMRESGFEPDSATFVSLLSACAQTGAVSLGSWVHQYIISEGLDLNVKLGT 247

Query: 280 SLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMR-AFGPR 339
           +LIN+Y+RCG+V KAREVFD MKE NV  WTAMIS YG HGYG+QA++LF +M    GP 
Sbjct: 248 ALINLYSRCGDVGKAREVFDKMKETNVAAWTAMISAYGTHGYGQQAVELFNKMEDDCGPI 307

Query: 340 PNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQ 399
           PNN+TFVAVLSACAH+GL+++GR V+  + ++Y L+PGVEHHVCMVDM GRAG L++AY+
Sbjct: 308 PNNVTFVAVLSACAHAGLVEEGRSVYKRMTKSYRLIPGVEHHVCMVDMLGRAGFLDEAYK 367

Query: 400 FIKKF--VPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYAL 459
           FI +     K   PA+WT+MLGAC+MH+N+DLGV++A+ ++A+EP+NPGH+VMLSNIYAL
Sbjct: 368 FIHQLDATGKATAPALWTAMLGACKMHRNYDLGVEIAKRLIALEPDNPGHHVMLSNIYAL 427

Query: 460 AGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGR 519
           +G+ D V  +R+ M+R  L+KQVGYS IE++ KTY+FSMGD+SH +T  IYRYL+ L+ R
Sbjct: 428 SGKTDEVSHIRDGMMRNNLRKQVGYSVIEVENKTYMFSMGDESHQETGEIYRYLETLISR 487

Query: 520 CSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDC 579
           C E GY P  E +MH +E+EE+++ALRYHSEKLA+AFGLL+T     I IVKNLRICEDC
Sbjct: 488 CKEIGYAPVSEEVMHQVEEEEKEFALRYHSEKLAVAFGLLKT-VDVAITIVKNLRICEDC 547

Query: 580 HSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           HSA K++SI+++R+I VRDK RFHHF++G+CSCLDYW
Sbjct: 548 HSAFKYISIVSNRQITVRDKLRFHHFQNGSCSCLDYW 583

BLAST of Lsi01G003140 vs. TAIR 10
Match: AT4G30700.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 476.1 bits (1224), Expect = 4.3e-134
Identity = 231/579 (39.90%), Postives = 359/579 (62.00%), Query Frame = 0

Query: 38  RLHSPVYEALLQAGPRLRNLQ---QVHAHIIVSGLQRSRSLLTKLLSLVCAAGSITYARR 97
           RL +     +L A   L+ L+   Q+H+    +G      +LT  +SL    G I     
Sbjct: 218 RLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSA 277

Query: 98  LFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLSA 157
           LF     PD   +++++   +  G +  ++  ++ ++ SG    + T  S++     L  
Sbjct: 278 LFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLML 337

Query: 158 LRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISG 217
           +     IH + +   + S   V  AL  +Y+K ++++ A+K+FD  PE+++ +WN++ISG
Sbjct: 338 I---YAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISG 397

Query: 218 YEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLN 277
           Y QNG  +++I +F  M +S F P+P TI  +LS+C+QLGAL  G W+HD      F+ +
Sbjct: 398 YTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESS 457

Query: 278 VVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRA 337
           + + T+LI MY +CG++++AR +FD M ++N VTW  MISGYG+HG G++A+ +F EM  
Sbjct: 458 IYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLN 517

Query: 338 FGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLN 397
            G  P  +TF+ VL AC+H+GL+ +G  +F+S+   YG  P V+H+ CMVD+ GRAG L 
Sbjct: 518 SGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQ 577

Query: 398 DAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIY 457
            A QFI+  +  EPG +VW ++LGACR+HK+ +L   V+E +  ++P+N G++V+LSNI+
Sbjct: 578 RALQFIEA-MSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELDPDNVGYHVLLSNIH 637

Query: 458 ALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELM 517
           +      +   VR    +R+L K  GY+ IEI    ++F+ GD+SHPQ   IY  L++L 
Sbjct: 638 SADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSHPQVKEIYEKLEKLE 697

Query: 518 GRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICE 577
           G+  E+GY P  E  +HD+E+EER+  ++ HSE+LA+AFGL+ T  G  IRI+KNLR+C 
Sbjct: 698 GKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEPGTEIRIIKNLRVCL 757

Query: 578 DCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
           DCH+  K +S I +R I+VRD  RFHHFKDG CSC DYW
Sbjct: 758 DCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792

BLAST of Lsi01G003140 vs. TAIR 10
Match: AT2G01510.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 6.2e-133
Identity = 234/591 (39.59%), Postives = 371/591 (62.77%), Query Frame = 0

Query: 25  LAFQHPVTRIFDARLHSPVYEALLQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVC 84
           LA Q P+T+         + E L  +  + + L+++HA ++ +G     SLLT+LL  + 
Sbjct: 2   LAKQTPLTK-------QMLSELLRASSSKPKQLKKIHAIVLRTGFSEKNSLLTQLLENLV 61

Query: 85  AAGSITYARRLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTS 144
             G + YAR++F  +  P  FL+++L K   +     +++L Y+ M   GV    +T+  
Sbjct: 62  VIGDMCYARQVFDEMHKPRIFLWNTLFKGYVRNQLPFESLLLYKKMRDLGVRPDEFTYPF 121

Query: 145 VIKACADLSALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERT 204
           V+KA + L     G  +H+HV+  G+G    V   LV +Y K  ++  A+ +F+ M  + 
Sbjct: 122 VVKAISQLGDFSCGFALHAHVVKYGFGCLGIVATELVMMYMKFGELSSAEFLFESMQVKD 181

Query: 205 IIAWNSLISGYEQNGFPKESIGVFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHD 264
           ++AWN+ ++   Q G    ++  F+ M     Q D  T+VS+LS+C QLG+L+ G  ++D
Sbjct: 182 LVAWNAFLAVCVQTGNSAIALEYFNKMCADAVQFDSFTVVSMLSACGQLGSLEIGEEIYD 241

Query: 265 YANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQ 324
            A  +  D N+++  + ++M+ +CGN   AR +F+ MK+RNVV+W+ MI GY M+G   +
Sbjct: 242 RARKEEIDCNIIVENARLDMHLKCGNTEAARVLFEEMKQRNVVSWSTMIVGYAMNGDSRE 301

Query: 325 AMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRGVFSSIKEA--YGLVPGVEHHVC 384
           A+ LF  M+  G RPN +TF+ VLSAC+H+GL+++G+  FS + ++    L P  EH+ C
Sbjct: 302 ALTLFTTMQNEGLRPNYVTFLGVLSACSHAGLVNEGKRYFSLMVQSNDKNLEPRKEHYAC 361

Query: 385 MVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPE 444
           MVD+ GR+GLL +AY+FIKK +P EP   +W ++LGAC +H++  LG KVA+ ++   P+
Sbjct: 362 MVDLLGRSGLLEEAYEFIKK-MPVEPDTGIWGALLGACAVHRDMILGQKVADVLVETAPD 421

Query: 445 NPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQ 504
              ++V+LSNIYA AG+ D V+ VR+ M +   KK   YS++E + K + F+ GDKSHPQ
Sbjct: 422 IGSYHVLLSNIYAAAGKWDCVDKVRSKMRKLGTKKVAAYSSVEFEGKIHFFNRGDKSHPQ 481

Query: 505 TNTIYRYLDELMGRCSESGYVPALESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGD 564
           +  IY  LDE++ +  + GYVP   S+ HD+E EE++ +L +HSEKLA+AFGL++   G 
Sbjct: 482 SKAIYEKLDEILKKIRKMGYVPDTCSVFHDVEMEEKECSLSHHSEKLAIAFGLIKGRPGH 541

Query: 565 TIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHHFKDGACSCLDYW 614
            IR++KNLR C+DCH+  K VS +   EII+RDK RFHHF++G CSC ++W
Sbjct: 542 PIRVMKNLRTCDDCHAFSKFVSSLTSTEIIMRDKNRFHHFRNGVCSCKEFW 584

BLAST of Lsi01G003140 vs. TAIR 10
Match: AT4G21065.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 471.5 bits (1212), Expect = 1.1e-132
Identity = 236/568 (41.55%), Postives = 366/568 (64.44%), Query Frame = 0

Query: 54  LRNLQQVHAHII-----VSGLQRSRSLLTKLLSLVCAAGSITYARRLFPTVPNP-DSFLF 113
           +  L+Q+HA  I     +S  +  + L+  L+SL  +   ++YA ++F  +  P + F++
Sbjct: 30  ITKLRQIHAFSIRHGVSISDAELGKHLIFYLVSLP-SPPPMSYAHKVFSKIEKPINVFIW 89

Query: 114 DSLLKATSKFGFSIDAVLFYRHMLFSG-VPRSNYTFTSVIKACADLSALRLGREIHSHVM 173
           ++L++  ++ G SI A   YR M  SG V    +T+  +IKA   ++ +RLG  IHS V+
Sbjct: 90  NTLIRGYAEIGNSISAFSLYREMRVSGLVEPDTHTYPFLIKAVTTMADVRLGETIHSVVI 149

Query: 174 VCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNSLISGYEQNGFPKESIG 233
             G+GS +YVQ +L+ LYA   D+  A KVFD MPE+ ++AWNS+I+G+ +NG P+E++ 
Sbjct: 150 RSGFGSLIYVQNSLLHLYANCGDVASAYKVFDKMPEKDLVAWNSVINGFAENGKPEEALA 209

Query: 234 VFHLMLESGFQPDPTTIVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYT 293
           ++  M   G +PD  TIVSLLS+C+++GAL  G  +H Y    G   N+     L+++Y 
Sbjct: 210 LYTEMNSKGIKPDGFTIVSLLSACAKIGALTLGKRVHVYMIKVGLTRNLHSSNVLLDLYA 269

Query: 294 RCGNVSKAREVFDSMKERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAF-GPRPNNITFV 353
           RCG V +A+ +FD M ++N V+WT++I G  ++G+G++A++LF  M +  G  P  ITFV
Sbjct: 270 RCGRVEEAKTLFDEMVDKNSVSWTSLIVGLAVNGFGKEAIELFKYMESTEGLLPCEITFV 329

Query: 354 AVLSACAHSGLIDDGRGVFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVP 413
            +L AC+H G++ +G   F  ++E Y + P +EH  CMVD+  RAG +  AY++IK  +P
Sbjct: 330 GILYACSHCGMVKEGFEYFRRMREEYKIEPRIEHFGCMVDLLARAGQVKKAYEYIKS-MP 389

Query: 414 KEPGPAVWTSMLGACRMHKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEM 473
            +P   +W ++LGAC +H + DL       +L +EP + G YV+LSN+YA   R   V+ 
Sbjct: 390 MQPNVVIWRTLLGACTVHGDSDLAEFARIQILQLEPNHSGDYVLLSNMYASEQRWSDVQK 449

Query: 474 VRNMMIRRRLKKQVGYSTIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPA 533
           +R  M+R  +KK  G+S +E+  + + F MGDKSHPQ++ IY  L E+ GR    GYVP 
Sbjct: 450 IRKQMLRDGVKKVPGHSLVEVGNRVHEFLMGDKSHPQSDAIYAKLKEMTGRLRSEGYVPQ 509

Query: 534 LESLMHDLEDEERDYALRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSI 593
           + ++  D+E+EE++ A+ YHSEK+A+AF L+ T +   I +VKNLR+C DCH AIK VS 
Sbjct: 510 ISNVYVDVEEEEKENAVVYHSEKIAIAFMLISTPERSPITVVKNLRVCADCHLAIKLVSK 569

Query: 594 IADREIIVRDKFRFHHFKDGACSCLDYW 614
           + +REI+VRD+ RFHHFK+G+CSC DYW
Sbjct: 570 VYNREIVVRDRSRFHHFKNGSCSCQDYW 595

BLAST of Lsi01G003140 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 470.7 bits (1210), Expect = 1.8e-132
Identity = 236/612 (38.56%), Postives = 376/612 (61.44%), Query Frame = 0

Query: 39  LHSPVYEAL--LQAGPRLRNLQQVHAHIIVSGLQRSRSLLTKLLSLVCAAGS---ITYAR 98
           L   +YE +  LQ   +   L+Q+HA ++ +GL +    +TK LS   ++ S   + YA+
Sbjct: 10  LEHNLYETMSCLQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQ 69

Query: 99  RLFPTVPNPDSFLFDSLLKATSKFGFSIDAVLFYRHMLFSGVPRSNYTFTSVIKACADLS 158
            +F     PD+FL++ +++  S       ++L Y+ ML S  P + YTF S++KAC++LS
Sbjct: 70  IVFDGFDRPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLS 129

Query: 159 ALRLGREIHSHVMVCGYGSDMYVQAALVALYAKASDMKVAKKVFDVMPERTIIAWNS--- 218
           A     +IH+ +   GY +D+Y   +L+  YA   + K+A  +FD +PE   ++WNS   
Sbjct: 130 AFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIK 189

Query: 219 ----------------------------LISGYEQNGFPKESIGVFHLMLESGFQPDPTT 278
                                       +ISGY Q    KE++ +FH M  S  +PD  +
Sbjct: 190 GYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVS 249

Query: 279 IVSLLSSCSQLGALDFGCWLHDYANGKGFDLNVVLGTSLINMYTRCGNVSKAREVFDSMK 338
           + + LS+C+QLGAL+ G W+H Y N     ++ VLG  LI+MY +CG + +A EVF ++K
Sbjct: 250 LANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIK 309

Query: 339 ERNVVTWTAMISGYGMHGYGEQAMKLFGEMRAFGPRPNNITFVAVLSACAHSGLIDDGRG 398
           +++V  WTA+ISGY  HG+G +A+  F EM+  G +PN ITF AVL+AC+++GL+++G+ 
Sbjct: 310 KKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKL 369

Query: 399 VFSSIKEAYGLVPGVEHHVCMVDMFGRAGLLNDAYQFIKKFVPKEPGPAVWTSMLGACRM 458
           +F S++  Y L P +EH+ C+VD+ GRAGLL++A +FI++ +P +P   +W ++L ACR+
Sbjct: 370 IFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQE-MPLKPNAVIWGALLKACRI 429

Query: 459 HKNFDLGVKVAEHVLAVEPENPGHYVMLSNIYALAGRMDRVEMVRNMMIRRRLKKQVGYS 518
           HKN +LG ++ E ++A++P + G YV  +NI+A+  + D+    R +M  + + K  G S
Sbjct: 430 HKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCS 489

Query: 519 TIEIKRKTYLFSMGDKSHPQTNTIYRYLDELMGRCSESGYVPALESLMHDL-EDEERDYA 578
           TI ++  T+ F  GD+SHP+   I      +  +  E+GYVP LE ++ DL +D+ER+  
Sbjct: 490 TISLEGTTHEFLAGDRSHPEIEKIQSKWRIMRRKLEENGYVPELEEMLLDLVDDDEREAI 549

Query: 579 LRYHSEKLALAFGLLETNQGDTIRIVKNLRICEDCHSAIKHVSIIADREIIVRDKFRFHH 614
           +  HSEKLA+ +GL++T  G  IRI+KNLR+C+DCH   K +S I  R+I++RD+ RFHH
Sbjct: 550 VHQHSEKLAITYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHH 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930115.5e-21961.18Pentatricopeptide repeat-containing protein At2g33760 OS=Arabidopsis thaliana OX... [more]
Q9SUH66.0e-13339.90Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana OX... [more]
Q9ZVF48.7e-13239.59Pentatricopeptide repeat-containing protein At2g01510, mitochondrial OS=Arabidop... [more]
A8MQA31.5e-13141.55Pentatricopeptide repeat-containing protein At4g21065 OS=Arabidopsis thaliana OX... [more]
Q9FJY72.5e-13138.56Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A5D3CPN10.0e+0091.15Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S4DTH40.0e+0091.15pentatricopeptide repeat-containing protein At2g33760 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1EL350.0e+0088.06pentatricopeptide repeat-containing protein At2g33760 isoform X1 OS=Cucurbita mo... [more]
A0A6J1KND50.0e+0088.61pentatricopeptide repeat-containing protein At2g33760 OS=Cucurbita maxima OX=366... [more]
A0A0A0KK640.0e+0088.04DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G5173... [more]
Match NameE-valueIdentityDescription
XP_038881818.10.0e+0094.29pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Benincasa hisp... [more]
XP_038881841.10.0e+0094.49pentatricopeptide repeat-containing protein At2g33760 isoform X3 [Benincasa hisp... [more]
XP_004134955.10.0e+0091.65pentatricopeptide repeat-containing protein At2g33760 isoform X1 [Cucumis sativu... [more]
XP_016899291.10.0e+0091.15PREDICTED: pentatricopeptide repeat-containing protein At2g33760 [Cucumis melo] ... [more]
XP_038881840.10.0e+0090.38pentatricopeptide repeat-containing protein At2g33760 isoform X2 [Benincasa hisp... [more]
Match NameE-valueIdentityDescription
AT2G33760.13.9e-22061.18Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G30700.14.3e-13439.90Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G01510.16.2e-13339.59Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G21065.11.1e-13241.55Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G66520.11.8e-13238.56Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 307..340
e-value: 3.6E-7
score: 27.9
coord: 207..239
e-value: 5.9E-7
score: 27.3
coord: 279..307
e-value: 3.9E-6
score: 24.7
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 207..236
e-value: 4.4E-7
score: 29.7
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 304..352
e-value: 4.9E-12
score: 45.9
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 274..304
score: 9.328124
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 204..238
score: 11.575191
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 305..339
score: 12.046526
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 153..255
e-value: 5.9E-19
score: 70.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 256..370
e-value: 1.4E-29
score: 105.5
coord: 371..523
e-value: 3.4E-8
score: 35.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 35..152
e-value: 1.6E-9
score: 39.6
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 284..462
IPR032867DYW domainPFAMPF14432DYW_deaminasecoord: 480..603
e-value: 1.0E-39
score: 135.3
NoneNo IPR availablePANTHERPTHR47924:SF35SUBFAMILY NOT NAMEDcoord: 38..607
NoneNo IPR availablePANTHERPTHR47924PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 38..607

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G003140.1Lsi01G003140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding