Lsi01G002530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G002530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAdenylate-forming reductase 06235
Locationchr01: 2290852 .. 2292492 (+)
RNA-Seq ExpressionLsi01G002530
SyntenyLsi01G002530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAACGCCCGTAAGGTTCTCGAGCTGTAGAGGCGTTGCCTTTGAAGTGAAACCTCATGACCATCACTTCTCCATCTCAAAACCCATGTCCCCACCATACCCAATTAGCAGCATCACCACCTTCTGGCCCCGCCCCAACTCTTTTCAAATATTCCCTTCCCCATCCCCTTCTTCACTTCTAAGATCCCTAAGCAAACCCAGCAGCCATTTCTGTGACGTCGATCCCCTCTCCGACCAAGATGAAGAACAACACCCCCAATACGACCTCCAAGCACTAGAAGAAGGCGATCAACAGAACCAAAGTACCCCAAAAAACCCGCCCTCTCAGGCTCATCCGAAGAACCCAAAATCCAGACTCTCCGTCATTCTCCTCGACCAAGGCCTCTTCACTGTCTACAAAAGGCTCTTTCTTCTCTCTCTTGCATTAAATATTGCCGCCCTCATTCTTGCTGCCACTGGGTATTTCCCATATGCCAGAGCTAGCCCGGCTCTGTTTTCAATCGGCAACATATTCGCCTTGACGCTTTGCCGGAGCGAGGCATTTCTGCGTGTACTGTTTTGGTTAGCAGTCACTATCTTTGGCAGATCATGGGTCCCACTAGCTTTCAAAACCGCCGTAACTTCTCTCCTCCAGAGCCTTGGTGGGGTCCACAGCGGTTGTGGAGTTTCGTCCATCGCCTGGCTTGTCTATGCCTTGTTACTCACTCTTATGGACCCACAGAAAAGATCCCCAGCCATCATTGTTGTTGCGTCCGCAATTCTAGCCCTTCTCTGTCTCTCTTCATTGGCCGCATTCCCTCTCGTACGCCATCTCCATCACAATGTCTTCGAGCGGACGCACCGTTTTGCTGGCTGGACCGCTCTCGCCCTCCTCTGGGCCTTCCTAATTCTAACACTCACTTACGATCCCATCACCAATTCATACAATAAAGACCTTGCTTCCCGTTTGCCCCATACACAGGAATTTTGGTTCACAACCGCAATCACTTTCCTAATCATTCTCCCATGGGTTACAGTTAGGCGCGTCCCTGTTCAAATCTCTGCCCCCTCTGGCCATGCCTCCATTATCAAATTCAGCGGCGGGGTAAAGCCCGGGTTATTGGGTCGGATCAGCCCGTCGCCATTATCGGAGTGGCATGCCTTTGGGATCATTTCCGACGGTGAAAAAGAGCACATGATGTTAGCCGGAGCGGTTGGGGATTTCACGAAATCCTTGGTGTCGGACCCACCCAGCCACTTATGGGTCCGGGGGGTCCACTTCGCCGGACTTCCGTATTTGGTGAACATGTACGAGAGAGCTCTGGTGGTGGCGACGGGATCTGGGATTTGCGTGTTTTTGTCGTTTTTGCTGCAGAGAAGTAGGGCTGACGTGCATTTGGTATGGGTGGCTAAAGGGATCGAGGAGAATTTCGGGAAGGAGATTAAGGAGATGGTGAATGGGTACCCGAAGGAGAAGGTGATAGTTCACGATACGGCGGTTTTGGGGCGGCCGAATGTGGCGGAGCTGACGGTGAAGGCGGCGGGAGAGTGGAAAGCGGAGGTTGTGATTGTTACGAGTAATCCGGAAGGGAGTAGAGATGTGGTGAATGCATGCAAAAGCGCTGGAATTGCAGCTTTTGGTCCAATTTGGGACTCTTGA

mRNA sequence

ATGCAAACGCCCGTAAGGTTCTCGAGCTGTAGAGGCGTTGCCTTTGAAGTGAAACCTCATGACCATCACTTCTCCATCTCAAAACCCATGTCCCCACCATACCCAATTAGCAGCATCACCACCTTCTGGCCCCGCCCCAACTCTTTTCAAATATTCCCTTCCCCATCCCCTTCTTCACTTCTAAGATCCCTAAGCAAACCCAGCAGCCATTTCTGTGACGTCGATCCCCTCTCCGACCAAGATGAAGAACAACACCCCCAATACGACCTCCAAGCACTAGAAGAAGGCGATCAACAGAACCAAAGTACCCCAAAAAACCCGCCCTCTCAGGCTCATCCGAAGAACCCAAAATCCAGACTCTCCGTCATTCTCCTCGACCAAGGCCTCTTCACTGTCTACAAAAGGCTCTTTCTTCTCTCTCTTGCATTAAATATTGCCGCCCTCATTCTTGCTGCCACTGGGTATTTCCCATATGCCAGAGCTAGCCCGGCTCTGTTTTCAATCGGCAACATATTCGCCTTGACGCTTTGCCGGAGCGAGGCATTTCTGCGTGTACTGTTTTGGTTAGCAGTCACTATCTTTGGCAGATCATGGGTCCCACTAGCTTTCAAAACCGCCGTAACTTCTCTCCTCCAGAGCCTTGGTGGGGTCCACAGCGGTTGTGGAGTTTCGTCCATCGCCTGGCTTGTCTATGCCTTGTTACTCACTCTTATGGACCCACAGAAAAGATCCCCAGCCATCATTGTTGTTGCGTCCGCAATTCTAGCCCTTCTCTGTCTCTCTTCATTGGCCGCATTCCCTCTCGTACGCCATCTCCATCACAATGTCTTCGAGCGGACGCACCGTTTTGCTGGCTGGACCGCTCTCGCCCTCCTCTGGGCCTTCCTAATTCTAACACTCACTTACGATCCCATCACCAATTCATACAATAAAGACCTTGCTTCCCGTTTGCCCCATACACAGGAATTTTGGTTCACAACCGCAATCACTTTCCTAATCATTCTCCCATGGGTTACAGTTAGGCGCGTCCCTGTTCAAATCTCTGCCCCCTCTGGCCATGCCTCCATTATCAAATTCAGCGGCGGGGTAAAGCCCGGGTTATTGGGTCGGATCAGCCCGTCGCCATTATCGGAGTGGCATGCCTTTGGGATCATTTCCGACGGTGAAAAAGAGCACATGATGTTAGCCGGAGCGGTTGGGGATTTCACGAAATCCTTGGTGTCGGACCCACCCAGCCACTTATGGGTCCGGGGGGTCCACTTCGCCGGACTTCCGTATTTGGTGAACATGTACGAGAGAGCTCTGGTGGTGGCGACGGGATCTGGGATTTGCGTGTTTTTGTCGTTTTTGCTGCAGAGAAGTAGGGCTGACGTGCATTTGGTATGGGTGGCTAAAGGGATCGAGGAGAATTTCGGGAAGGAGATTAAGGAGATGGTGAATGGGTACCCGAAGGAGAAGGTGATAGTTCACGATACGGCGGTTTTGGGGCGGCCGAATGTGGCGGAGCTGACGGTGAAGGCGGCGGGAGAGTGGAAAGCGGAGGTTGTGATTGTTACGAGTAATCCGGAAGGGAGTAGAGATGTGGTGAATGCATGCAAAAGCGCTGGAATTGCAGCTTTTGGTCCAATTTGGGACTCTTGA

Coding sequence (CDS)

ATGCAAACGCCCGTAAGGTTCTCGAGCTGTAGAGGCGTTGCCTTTGAAGTGAAACCTCATGACCATCACTTCTCCATCTCAAAACCCATGTCCCCACCATACCCAATTAGCAGCATCACCACCTTCTGGCCCCGCCCCAACTCTTTTCAAATATTCCCTTCCCCATCCCCTTCTTCACTTCTAAGATCCCTAAGCAAACCCAGCAGCCATTTCTGTGACGTCGATCCCCTCTCCGACCAAGATGAAGAACAACACCCCCAATACGACCTCCAAGCACTAGAAGAAGGCGATCAACAGAACCAAAGTACCCCAAAAAACCCGCCCTCTCAGGCTCATCCGAAGAACCCAAAATCCAGACTCTCCGTCATTCTCCTCGACCAAGGCCTCTTCACTGTCTACAAAAGGCTCTTTCTTCTCTCTCTTGCATTAAATATTGCCGCCCTCATTCTTGCTGCCACTGGGTATTTCCCATATGCCAGAGCTAGCCCGGCTCTGTTTTCAATCGGCAACATATTCGCCTTGACGCTTTGCCGGAGCGAGGCATTTCTGCGTGTACTGTTTTGGTTAGCAGTCACTATCTTTGGCAGATCATGGGTCCCACTAGCTTTCAAAACCGCCGTAACTTCTCTCCTCCAGAGCCTTGGTGGGGTCCACAGCGGTTGTGGAGTTTCGTCCATCGCCTGGCTTGTCTATGCCTTGTTACTCACTCTTATGGACCCACAGAAAAGATCCCCAGCCATCATTGTTGTTGCGTCCGCAATTCTAGCCCTTCTCTGTCTCTCTTCATTGGCCGCATTCCCTCTCGTACGCCATCTCCATCACAATGTCTTCGAGCGGACGCACCGTTTTGCTGGCTGGACCGCTCTCGCCCTCCTCTGGGCCTTCCTAATTCTAACACTCACTTACGATCCCATCACCAATTCATACAATAAAGACCTTGCTTCCCGTTTGCCCCATACACAGGAATTTTGGTTCACAACCGCAATCACTTTCCTAATCATTCTCCCATGGGTTACAGTTAGGCGCGTCCCTGTTCAAATCTCTGCCCCCTCTGGCCATGCCTCCATTATCAAATTCAGCGGCGGGGTAAAGCCCGGGTTATTGGGTCGGATCAGCCCGTCGCCATTATCGGAGTGGCATGCCTTTGGGATCATTTCCGACGGTGAAAAAGAGCACATGATGTTAGCCGGAGCGGTTGGGGATTTCACGAAATCCTTGGTGTCGGACCCACCCAGCCACTTATGGGTCCGGGGGGTCCACTTCGCCGGACTTCCGTATTTGGTGAACATGTACGAGAGAGCTCTGGTGGTGGCGACGGGATCTGGGATTTGCGTGTTTTTGTCGTTTTTGCTGCAGAGAAGTAGGGCTGACGTGCATTTGGTATGGGTGGCTAAAGGGATCGAGGAGAATTTCGGGAAGGAGATTAAGGAGATGGTGAATGGGTACCCGAAGGAGAAGGTGATAGTTCACGATACGGCGGTTTTGGGGCGGCCGAATGTGGCGGAGCTGACGGTGAAGGCGGCGGGAGAGTGGAAAGCGGAGGTTGTGATTGTTACGAGTAATCCGGAAGGGAGTAGAGATGTGGTGAATGCATGCAAAAGCGCTGGAATTGCAGCTTTTGGTCCAATTTGGGACTCTTGA

Protein sequence

MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISSITTFWPRPNSFQIFPSPSPSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHPKNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLLTLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSAGIAAFGPIWDS
Homology
BLAST of Lsi01G002530 vs. ExPASy Swiss-Prot
Match: A8NVB7 (Adenylate-forming reductase 06235 OS=Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) OX=240176 GN=CC1G_06235 PE=2 SV=3)

HSP 1 Score: 221.1 bits (562), Expect = 3.1e-56
Identity = 152/443 (34.31%), Postives = 233/443 (52.60%), Query Frame = 0

Query: 113  PKNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALI-LAATGYFPYARASPALFSIGNI 172
            P+  + R+ V  L   +F +Y+RLF +   +N+A  I +  T  +   R    +  + N+
Sbjct: 597  PEKTQGRI-VRNLRHQIFNLYRRLFSVVFIINMALFIWILVTKDYDAHRLGGIV--VANV 656

Query: 173  FALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLV- 232
            F   L R E  +  LF +  T    SW PL  +  V + + ++GG+HSG GVS+  WLV 
Sbjct: 657  FIGVLMRQEMVINTLF-IIFTAVPPSW-PLCIR-RVCARIYTIGGIHSGAGVSAFVWLVA 716

Query: 233  YALLLT--LMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTA 292
            +    T  +++  K S   + +   ILA L    + A+P +R   H+ FE THR+ GWTA
Sbjct: 717  FTAQATKEMINKGKTSVRTVAITYVILAELLGILIFAYPALRKKMHDTFENTHRYLGWTA 776

Query: 293  LALLW-AFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQI 352
            LAL+W  F+ LT+ Y P   +  + L  + PH   FW     T  II PW  +R+V  + 
Sbjct: 777  LALVWIQFMFLTIDYLPEGQTLGQTLV-KSPH---FWLVIIFTISIIWPWFRLRKVDCRP 836

Query: 353  SAPSGHASIIKFSGGVKP--GLLGRISPSPLSEWHAFGIIS-DGEKEHMMLAGAVGDFTK 412
               S HA  + F  GV P  G   R+S +PL EWH F  IS  G   + ++    GD+T 
Sbjct: 837  EVLSNHAVRLWFDYGVTPDAGTFVRLSDAPLKEWHGFASISIPGRTGYSVVVSRAGDWTS 896

Query: 413  SLVSDPPSHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVA 472
              ++DPP+ +WV+GV   G+  LV M+ R ++VATGSGI      +L+R +  + ++W A
Sbjct: 897  KHINDPPTKMWVKGVPTYGVLKLVPMFRRMVLVATGSGIGPCAPAILRR-QIPMRVLWTA 956

Query: 473  KGIEENFGKEI-KEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPE 532
              + E FG  +   ++   P    +++DT   G+P++ +L ++   E+ AE V + SN  
Sbjct: 957  PNVRETFGDNLCNSILEACP--DAVIYDTRKHGKPDMVKLVLRLVKEFDAEAVAIISNQP 1016

Query: 533  GSRDVVNACKSAGIAAFGPIWDS 547
             +  VV  C S GI AFG IWDS
Sbjct: 1017 LTEKVVYGCMSRGIPAFGAIWDS 1026

BLAST of Lsi01G002530 vs. ExPASy Swiss-Prot
Match: A8NS27 (Adenylate-forming reductase 03009 OS=Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) OX=240176 GN=CC1G_03009 PE=2 SV=3)

HSP 1 Score: 219.5 bits (558), Expect = 9.0e-56
Identity = 152/441 (34.47%), Postives = 228/441 (51.70%), Query Frame = 0

Query: 112  HPKNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNI 171
            HP+  + RL V  L   +F++Y+RLF +   +N A LI        Y         I N+
Sbjct: 596  HPEKTQGRL-VRNLRHQIFSLYRRLFSVVFIVNAAILIWICVKK-EYDANRIGGIVIANV 655

Query: 172  FALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWL-V 231
            F   L R E  +   F+L  T    SW     +TA  + +  +GG+HSG GVSS+ WL +
Sbjct: 656  FIGVLMRQELVINT-FFLIFTSIPSSWPLFIRRTA--ARVYHIGGIHSGAGVSSLLWLCL 715

Query: 232  YALLLT--LMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTA 291
            +    T  +++  K S   + +   ILA L    + A+P +R   H+ FE THRF GW+A
Sbjct: 716  FTAQATKEMINGGKTSVRTVAITYVILAELLGIVIFAYPALRKRLHDTFENTHRFLGWSA 775

Query: 292  LALLWA-FLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQI 351
            LAL+W  F+ LT+ Y P      + L   L  T +FW    +T  +I PW  +R+V V+ 
Sbjct: 776  LALVWVQFMFLTIDYLP----EGQMLGQTLVKTPQFWLVIILTCSVIWPWFRLRKVDVKP 835

Query: 352  SAPSGHASIIKFSGGVKP-GLLGRISPSPLSEWHAFGIIS-DGEKEHMMLAGAVGDFTKS 411
               S HA  + F     P G   R+S +PL EWH F  I   G   + ++    GD+T  
Sbjct: 836  EVLSNHAVRLWFDYVTPPAGTFMRVSDAPLKEWHGFAAIPIPGRTGYSLVVSRAGDWTSK 895

Query: 412  LVSDPPSHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAK 471
             +++PP+ LWV+GV   G+  LV M+ R ++VATGSGI      + ++ R  + ++W A 
Sbjct: 896  HIANPPTKLWVKGVPTYGVLKLVPMFRRMVIVATGSGIGPCAPAIFEK-RIPMRVLWTAP 955

Query: 472  GIEENFGKEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGS 531
             + E FG ++ + +     E VI +DT   G+P++ +LT++   E+ AE V + SN   +
Sbjct: 956  NVRETFGDKLVDSILEANPEAVI-YDTRKHGKPDMVKLTLRLVKEFNAEAVAIISNQPLT 1015

Query: 532  RDVVNACKSAGIAAFGPIWDS 547
              VV    S GI AFG IWDS
Sbjct: 1016 EKVVYGMMSRGIPAFGAIWDS 1025

BLAST of Lsi01G002530 vs. ExPASy TrEMBL
Match: A0A5A7UGJ4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008080 PE=4 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.5e-268
Identity = 485/551 (88.02%), Postives = 505/551 (91.65%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISS----ITTFWPRPNSFQIFPSPS 60
           MQTPVRFSSCRGVAFEVKPH+HHFSISKP+SP +P +S      TFWPRPNSFQIFPSPS
Sbjct: 1   MQTPVRFSSCRGVAFEVKPHEHHFSISKPISPLHPSASSNITTNTFWPRPNSFQIFPSPS 60

Query: 61  PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNP-PSQAHPKN 120
           PSS+LRSLS+PSSHFCDV   SD  EEQ  +YD   LEEGD     TP+NP PS     N
Sbjct: 61  PSSILRSLSRPSSHFCDV---SDDQEEQ--EYD---LEEGDPH---TPQNPKPSHPPKNN 120

Query: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALT 180
           PKSRLSVILLDQGLFTVYKRLFLLSLALNI ALILAA GYFPYARA+P LFSIGNIFAL+
Sbjct: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNITALILAANGYFPYARANPTLFSIGNIFALS 180

Query: 181 LCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLL 240
           +CRSEAFLRVLFWLAVT+ GRSWVPLA KTAVTSLLQSLGGVHSGCGVSSIAWLVY+L+L
Sbjct: 181 VCRSEAFLRVLFWLAVTVLGRSWVPLALKTAVTSLLQSLGGVHSGCGVSSIAWLVYSLVL 240

Query: 241 TLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAF 300
           TL DP KRS  II VASAILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALALLWAF
Sbjct: 241 TLTDPLKRSTPIIAVASAILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALALLWAF 300

Query: 301 LILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHAS 360
           LILTLTYDPITNS+NK L SRL  TQEFWFTTAITFLIILPWVTVRRVPV+ISAPSGHAS
Sbjct: 301 LILTLTYDPITNSHNKHLVSRLFQTQEFWFTTAITFLIILPWVTVRRVPVRISAPSGHAS 360

Query: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLW 420
           IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PPSHLW
Sbjct: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPPSHLW 420

Query: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEI 480
           VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENFGKEI
Sbjct: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENFGKEI 480

Query: 481 KEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSA 540
           K MVNGYPKEKVI+HDTAVLGRPNVAELTVKAAGEWK EVVIVTSNPEGSRDVVNACKS+
Sbjct: 481 KGMVNGYPKEKVILHDTAVLGRPNVAELTVKAAGEWKTEVVIVTSNPEGSRDVVNACKSS 540

Query: 541 GIAAFGPIWDS 547
           GIAAFGPIWDS
Sbjct: 541 GIAAFGPIWDS 540

BLAST of Lsi01G002530 vs. ExPASy TrEMBL
Match: A0A1S3B084 (uncharacterized protein LOC103484460 OS=Cucumis melo OX=3656 GN=LOC103484460 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.9e-268
Identity = 485/551 (88.02%), Postives = 504/551 (91.47%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISS----ITTFWPRPNSFQIFPSPS 60
           MQTPVRFSSCRGVAFEVKPH+HHFSISKP+SP +P +S      TFWPRPNSFQIFPSPS
Sbjct: 1   MQTPVRFSSCRGVAFEVKPHEHHFSISKPISPLHPSASSNITTNTFWPRPNSFQIFPSPS 60

Query: 61  PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNP-PSQAHPKN 120
           PSS+LRSLS+PSSHFCDV   SD  EEQ  +YD   LEEGD     TP+NP PS     N
Sbjct: 61  PSSILRSLSRPSSHFCDV---SDDQEEQ--EYD---LEEGDPH---TPQNPKPSHPPKNN 120

Query: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALT 180
           PKSRLSVILLDQGLFTVYKRLFLLSLALNI ALILAA GYFPYARA+P LFSIGNIFAL+
Sbjct: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNITALILAANGYFPYARANPTLFSIGNIFALS 180

Query: 181 LCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLL 240
           +CRSEAFLRVLFWLAVT+ GRSWVPLA KTAVTSLLQSLGGVHSGCGVSSIAWLVY+L+L
Sbjct: 181 VCRSEAFLRVLFWLAVTVLGRSWVPLALKTAVTSLLQSLGGVHSGCGVSSIAWLVYSLVL 240

Query: 241 TLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAF 300
            L DP KRS  II VASAILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALALLWAF
Sbjct: 241 ALRDPLKRSTPIIAVASAILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALALLWAF 300

Query: 301 LILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHAS 360
           LILTLTYDPITNS+NK L SRL  TQEFWFTTAITFLIILPWVTVRRVPV+ISAPSGHAS
Sbjct: 301 LILTLTYDPITNSHNKHLVSRLFQTQEFWFTTAITFLIILPWVTVRRVPVRISAPSGHAS 360

Query: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLW 420
           IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PPSHLW
Sbjct: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPPSHLW 420

Query: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEI 480
           VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENFGKEI
Sbjct: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENFGKEI 480

Query: 481 KEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSA 540
           K MVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWK EVVIVTSNPEGSRDVVNACKS+
Sbjct: 481 KGMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKTEVVIVTSNPEGSRDVVNACKSS 540

Query: 541 GIAAFGPIWDS 547
           GIAAFGPIWDS
Sbjct: 541 GIAAFGPIWDS 540

BLAST of Lsi01G002530 vs. ExPASy TrEMBL
Match: A0A0A0KI87 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G519460 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.7e-263
Identity = 479/555 (86.31%), Postives = 504/555 (90.81%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSP---PYPISSITT---FWPRPNSFQIFPS 60
           M+TPVRFSSCRGVAFEVKPH+HHFSISKP+SP   P   S+ITT   FWPRPNSFQIFPS
Sbjct: 1   METPVRFSSCRGVAFEVKPHEHHFSISKPISPSSHPSASSNITTNTPFWPRPNSFQIFPS 60

Query: 61  PS-PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHP 120
           PS PSSLLRSLS+PSSHFCDV   SDQ+++   +YD   LEEGD     TP   P  +HP
Sbjct: 61  PSAPSSLLRSLSRPSSHFCDV---SDQEQQ---EYD---LEEGDPH---TPVQNPKPSHP 120

Query: 121 --KNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNI 180
              NPKSRLSVILLDQGLFTVYKRLFLL LALNI AL+LAATGYFPYARA+P++FSIGNI
Sbjct: 121 PKNNPKSRLSVILLDQGLFTVYKRLFLLCLALNITALVLAATGYFPYARANPSVFSIGNI 180

Query: 181 FALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY 240
            AL++CRSEAFLRV+FWLAVT+ GR WVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY
Sbjct: 181 LALSVCRSEAFLRVVFWLAVTVLGRPWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY 240

Query: 241 ALLLTLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALAL 300
           AL+LTL DP  RS  II VAS+ILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALAL
Sbjct: 241 ALVLTLTDPFNRSTPIIAVASSILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALAL 300

Query: 301 LWAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPS 360
           LWAFLILTLTYDPITNSYNKD+ SRL  TQEFWFT AITFLIILPWVTVRRVPVQISAPS
Sbjct: 301 LWAFLILTLTYDPITNSYNKDVPSRLFQTQEFWFTAAITFLIILPWVTVRRVPVQISAPS 360

Query: 361 GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPP 420
           GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PP
Sbjct: 361 GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPP 420

Query: 421 SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENF 480
           SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENF
Sbjct: 421 SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENF 480

Query: 481 GKEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNA 540
           GKEIK MVNGYPKEKVIVHDTAVLGRPNVAEL+V AAG+WK EVVIVTSNPEGSRDVVNA
Sbjct: 481 GKEIKGMVNGYPKEKVIVHDTAVLGRPNVAELSVMAAGKWKTEVVIVTSNPEGSRDVVNA 540

Query: 541 CKSAGIAAFGPIWDS 547
           CKSAGIAAFGPIWDS
Sbjct: 541 CKSAGIAAFGPIWDS 543

BLAST of Lsi01G002530 vs. ExPASy TrEMBL
Match: A0A6J1EEE6 (uncharacterized protein LOC111433565 OS=Cucurbita moschata OX=3662 GN=LOC111433565 PE=4 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.5e-234
Identity = 432/554 (77.98%), Postives = 464/554 (83.75%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISSITTFWPRPNSFQIFPSPSPSSL 60
           M TPVRFSSCRGVAFEVK  +H  S    +       S TTF             S SSL
Sbjct: 1   MATPVRFSSCRGVAFEVKAQEHQLSHQSSV-------SFTTF-----------LSSSSSL 60

Query: 61  LRSLSKPSSHFCDVD-------PLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHP 120
            RSLSKPS+HFCD+D       PLSD+DE      +LQ+LE+G          PP    P
Sbjct: 61  RRSLSKPSTHFCDIDPDQQKLQPLSDEDEN-----ELQSLEQGHY------VPPPQNPSP 120

Query: 121 KNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFA 180
            NPKSRLSVILLDQGLFTVYKRLF+LS++LNI ALILA T  FPYAR +PALFSIGNIF 
Sbjct: 121 PNPKSRLSVILLDQGLFTVYKRLFILSVSLNITALILALTRNFPYARNNPALFSIGNIFM 180

Query: 181 LTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYAL 240
           L LCRSEAFLRVLFWL+V+I GRSWVPL  KT++TSLLQSLGG+HSGCGVSSIAWLVYAL
Sbjct: 181 LCLCRSEAFLRVLFWLSVSIIGRSWVPLCVKTSLTSLLQSLGGIHSGCGVSSIAWLVYAL 240

Query: 241 LLTLM-DPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALL 300
           L+ ++ DP+  SPA+I VASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGW ALALL
Sbjct: 241 LIQILEDPKNASPAMIAVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWAALALL 300

Query: 301 WAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSG 360
           WAF+IL LTYDPIT SY   L SRL   QEFWFT AITFLIILPWVTVRRVPVQISAPSG
Sbjct: 301 WAFIILKLTYDPITKSYTNPLGSRLVKNQEFWFTAAITFLIILPWVTVRRVPVQISAPSG 360

Query: 361 HASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPS 420
           HASIIKF+GGV+PGLLGRISPSPLSEWHAFGIISDG+KEHMMLAGAVGDFTKSLVS+PPS
Sbjct: 361 HASIIKFNGGVQPGLLGRISPSPLSEWHAFGIISDGQKEHMMLAGAVGDFTKSLVSNPPS 420

Query: 421 HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFG 480
           HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFL+QRSRADV+LVWVAKGIEENFG
Sbjct: 421 HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLIQRSRADVYLVWVAKGIEENFG 480

Query: 481 KEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNAC 540
           KEIKE VNGYPKEKVI+HDT V GRPNVAELTVKAAG+WKAEVVIVTSNPEGSRDVVNAC
Sbjct: 481 KEIKETVNGYPKEKVIIHDTKVSGRPNVAELTVKAAGKWKAEVVIVTSNPEGSRDVVNAC 525

Query: 541 KSAGIAAFGPIWDS 547
           K +GIAAFGPIWDS
Sbjct: 541 KRSGIAAFGPIWDS 525

BLAST of Lsi01G002530 vs. ExPASy TrEMBL
Match: A0A7J7C5Q7 (Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW21G01315 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.8e-220
Identity = 393/554 (70.94%), Postives = 462/554 (83.39%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPM-SPPYPISSITTFWPRPNSFQIFPSPSPSS 60
           MQ+PVRFSSCRGVAFE+KPH   FSI+KP  + P         W + NS +IF    PSS
Sbjct: 1   MQSPVRFSSCRGVAFEIKPHPDPFSITKPTEAEPQGSKRFWLPWVQRNSSKIF----PSS 60

Query: 61  LLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGD-QQNQSTP------KNPPSQAH 120
           + RS+S+PSSHFCD+D + D+DE++   + L+ LEEG   ++Q  P         PS+A 
Sbjct: 61  IQRSVSRPSSHFCDLD-IDDEDEDEE-DHVLERLEEGSTDEDQEKPISVPITSEQPSKAP 120

Query: 121 PKNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIF 180
           P+ P SRLSVILLDQGLFTVYKRLF++ LA+NI AL+LAATG FPYAR    LFSI NIF
Sbjct: 121 PRKPDSRLSVILLDQGLFTVYKRLFVVCLAVNITALVLAATGNFPYARNKATLFSIANIF 180

Query: 181 ALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYA 240
           ALTLCRSEAFLR++FWLAV + GRSW+PL  KTA TSLLQSLGG+HS CG+SS+AWL+YA
Sbjct: 181 ALTLCRSEAFLRIVFWLAVKVMGRSWIPLPIKTATTSLLQSLGGIHSSCGISSVAWLIYA 240

Query: 241 LLLTLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALL 300
           L+LTL + +  +P II VAS IL+LLCLS LAAFPLVRHLHHN+FERTHRFAGWT+L L+
Sbjct: 241 LVLTLKNRKDTTPEIIAVASTILSLLCLSCLAAFPLVRHLHHNIFERTHRFAGWTSLGLV 300

Query: 301 WAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSG 360
           WAF+ILT++YDP T SY+ D+ S+L   QEFWFT AIT LII+PWVTVRRVPV++SAPSG
Sbjct: 301 WAFVILTISYDPKTKSYSHDIGSKLVKQQEFWFTLAITVLIIIPWVTVRRVPVKVSAPSG 360

Query: 361 HASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPS 420
           HASIIKF GGVK G+LGRISPSP SEWHAFGIISDG+ EHMMLAGAVGDFTKSLV++PPS
Sbjct: 361 HASIIKFEGGVKSGILGRISPSPFSEWHAFGIISDGKSEHMMLAGAVGDFTKSLVANPPS 420

Query: 421 HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFG 480
           HLWVR VHFAGLPYLVNMY+R L+VATGSGICVFLSFLLQ S A+V ++WVAKGIE+NFG
Sbjct: 421 HLWVRQVHFAGLPYLVNMYDRVLLVATGSGICVFLSFLLQPSSANVCVLWVAKGIEQNFG 480

Query: 481 KEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNAC 540
           KEIKEM++G+PK+KVIVHDTAVLGRPNV+E++V AA +W AEVV+VTSNPEGSRDVVN+C
Sbjct: 481 KEIKEMMSGHPKDKVIVHDTAVLGRPNVSEMSVDAAKKWGAEVVVVTSNPEGSRDVVNSC 540

Query: 541 KSAGIAAFGPIWDS 547
           KSAGI AFGPIWDS
Sbjct: 541 KSAGIPAFGPIWDS 548

BLAST of Lsi01G002530 vs. NCBI nr
Match: XP_038883706.1 (adenylate-forming reductase 03009 [Benincasa hispida])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.7e-291
Identity = 510/546 (93.41%), Postives = 526/546 (96.34%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISSITTFWPRPNSFQIFPSPSPSSL 60
           MQTPVRFSSCRGVAFEVKPH+HHFSISKP+SPP+P SSITTFWPR NSFQIFPSPSPSSL
Sbjct: 1   MQTPVRFSSCRGVAFEVKPHEHHFSISKPISPPHPSSSITTFWPRRNSFQIFPSPSPSSL 60

Query: 61  LRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHPKNPKSRL 120
           LRSLSKPSSHFCDVDPLSD +EEQH QYDL+ALEEGDQQNQSTP+NPPSQ HPKNPKSRL
Sbjct: 61  LRSLSKPSSHFCDVDPLSD-EEEQHHQYDLEALEEGDQQNQSTPQNPPSQPHPKNPKSRL 120

Query: 121 SVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALTLCRSE 180
           SVILLDQGLFTVYKRLFLL L+LNI ALILA TGYFPYARA PALFSIGNIFALTLCRSE
Sbjct: 121 SVILLDQGLFTVYKRLFLLCLSLNITALILAVTGYFPYARARPALFSIGNIFALTLCRSE 180

Query: 181 AFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLLTLMDP 240
           AFLRV+FWL VTI GRSWVPLAFKTAVTS LQSLGGVHSGCGVSSIAWLVYAL LTLMD 
Sbjct: 181 AFLRVVFWLTVTILGRSWVPLAFKTAVTSFLQSLGGVHSGCGVSSIAWLVYALALTLMDA 240

Query: 241 QKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAFLILTL 300
           Q RSPAII VASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAFLILTL
Sbjct: 241 QTRSPAIIAVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAFLILTL 300

Query: 301 TYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHASIIKFS 360
           TYDPITNSYNKDLASRL  TQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHASIIKFS
Sbjct: 301 TYDPITNSYNKDLASRLLQTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHASIIKFS 360

Query: 361 GGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLWVRGVH 420
           GGV+PGLLGRISPSPLSEWHAFGIISDGEKEH+MLAGAVGDFTKSLVS+PP+HLWVRGVH
Sbjct: 361 GGVQPGLLGRISPSPLSEWHAFGIISDGEKEHIMLAGAVGDFTKSLVSNPPTHLWVRGVH 420

Query: 421 FAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEIKEMVN 480
           FAGLPYLVNMYER LVVATGSGICVFLSFLLQ+SRADVHLVWVAKGIEENFGKEIKEMVN
Sbjct: 421 FAGLPYLVNMYERTLVVATGSGICVFLSFLLQKSRADVHLVWVAKGIEENFGKEIKEMVN 480

Query: 481 GYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSAGIAAF 540
           G+P+EKVIVHDTAVLGRPNVAELTVKAA EWKAEVVIVTSNPEGSRDVVNACKS+GIAAF
Sbjct: 481 GHPREKVIVHDTAVLGRPNVAELTVKAAREWKAEVVIVTSNPEGSRDVVNACKSSGIAAF 540

Query: 541 GPIWDS 547
           GPIWDS
Sbjct: 541 GPIWDS 545

BLAST of Lsi01G002530 vs. NCBI nr
Match: KAA0052631.1 (uncharacterized protein E6C27_scaffold120G002410 [Cucumis melo var. makuwa] >TYK13196.1 uncharacterized protein E5676_scaffold255G008080 [Cucumis melo var. makuwa])

HSP 1 Score: 933.7 bits (2412), Expect = 7.1e-268
Identity = 485/551 (88.02%), Postives = 505/551 (91.65%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISS----ITTFWPRPNSFQIFPSPS 60
           MQTPVRFSSCRGVAFEVKPH+HHFSISKP+SP +P +S      TFWPRPNSFQIFPSPS
Sbjct: 1   MQTPVRFSSCRGVAFEVKPHEHHFSISKPISPLHPSASSNITTNTFWPRPNSFQIFPSPS 60

Query: 61  PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNP-PSQAHPKN 120
           PSS+LRSLS+PSSHFCDV   SD  EEQ  +YD   LEEGD     TP+NP PS     N
Sbjct: 61  PSSILRSLSRPSSHFCDV---SDDQEEQ--EYD---LEEGDPH---TPQNPKPSHPPKNN 120

Query: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALT 180
           PKSRLSVILLDQGLFTVYKRLFLLSLALNI ALILAA GYFPYARA+P LFSIGNIFAL+
Sbjct: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNITALILAANGYFPYARANPTLFSIGNIFALS 180

Query: 181 LCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLL 240
           +CRSEAFLRVLFWLAVT+ GRSWVPLA KTAVTSLLQSLGGVHSGCGVSSIAWLVY+L+L
Sbjct: 181 VCRSEAFLRVLFWLAVTVLGRSWVPLALKTAVTSLLQSLGGVHSGCGVSSIAWLVYSLVL 240

Query: 241 TLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAF 300
           TL DP KRS  II VASAILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALALLWAF
Sbjct: 241 TLTDPLKRSTPIIAVASAILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALALLWAF 300

Query: 301 LILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHAS 360
           LILTLTYDPITNS+NK L SRL  TQEFWFTTAITFLIILPWVTVRRVPV+ISAPSGHAS
Sbjct: 301 LILTLTYDPITNSHNKHLVSRLFQTQEFWFTTAITFLIILPWVTVRRVPVRISAPSGHAS 360

Query: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLW 420
           IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PPSHLW
Sbjct: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPPSHLW 420

Query: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEI 480
           VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENFGKEI
Sbjct: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENFGKEI 480

Query: 481 KEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSA 540
           K MVNGYPKEKVI+HDTAVLGRPNVAELTVKAAGEWK EVVIVTSNPEGSRDVVNACKS+
Sbjct: 481 KGMVNGYPKEKVILHDTAVLGRPNVAELTVKAAGEWKTEVVIVTSNPEGSRDVVNACKSS 540

Query: 541 GIAAFGPIWDS 547
           GIAAFGPIWDS
Sbjct: 541 GIAAFGPIWDS 540

BLAST of Lsi01G002530 vs. NCBI nr
Match: XP_008439758.1 (PREDICTED: uncharacterized protein LOC103484460 [Cucumis melo])

HSP 1 Score: 932.9 bits (2410), Expect = 1.2e-267
Identity = 485/551 (88.02%), Postives = 504/551 (91.47%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISS----ITTFWPRPNSFQIFPSPS 60
           MQTPVRFSSCRGVAFEVKPH+HHFSISKP+SP +P +S      TFWPRPNSFQIFPSPS
Sbjct: 1   MQTPVRFSSCRGVAFEVKPHEHHFSISKPISPLHPSASSNITTNTFWPRPNSFQIFPSPS 60

Query: 61  PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNP-PSQAHPKN 120
           PSS+LRSLS+PSSHFCDV   SD  EEQ  +YD   LEEGD     TP+NP PS     N
Sbjct: 61  PSSILRSLSRPSSHFCDV---SDDQEEQ--EYD---LEEGDPH---TPQNPKPSHPPKNN 120

Query: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFALT 180
           PKSRLSVILLDQGLFTVYKRLFLLSLALNI ALILAA GYFPYARA+P LFSIGNIFAL+
Sbjct: 121 PKSRLSVILLDQGLFTVYKRLFLLSLALNITALILAANGYFPYARANPTLFSIGNIFALS 180

Query: 181 LCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYALLL 240
           +CRSEAFLRVLFWLAVT+ GRSWVPLA KTAVTSLLQSLGGVHSGCGVSSIAWLVY+L+L
Sbjct: 181 VCRSEAFLRVLFWLAVTVLGRSWVPLALKTAVTSLLQSLGGVHSGCGVSSIAWLVYSLVL 240

Query: 241 TLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALLWAF 300
            L DP KRS  II VASAILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALALLWAF
Sbjct: 241 ALRDPLKRSTPIIAVASAILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALALLWAF 300

Query: 301 LILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSGHAS 360
           LILTLTYDPITNS+NK L SRL  TQEFWFTTAITFLIILPWVTVRRVPV+ISAPSGHAS
Sbjct: 301 LILTLTYDPITNSHNKHLVSRLFQTQEFWFTTAITFLIILPWVTVRRVPVRISAPSGHAS 360

Query: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPSHLW 420
           IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PPSHLW
Sbjct: 361 IIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPPSHLW 420

Query: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFGKEI 480
           VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENFGKEI
Sbjct: 421 VRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENFGKEI 480

Query: 481 KEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNACKSA 540
           K MVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWK EVVIVTSNPEGSRDVVNACKS+
Sbjct: 481 KGMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKTEVVIVTSNPEGSRDVVNACKSS 540

Query: 541 GIAAFGPIWDS 547
           GIAAFGPIWDS
Sbjct: 541 GIAAFGPIWDS 540

BLAST of Lsi01G002530 vs. NCBI nr
Match: XP_011658244.1 (adenylate-forming reductase 06235 [Cucumis sativus] >KGN49293.1 hypothetical protein Csa_003013 [Cucumis sativus])

HSP 1 Score: 916.4 bits (2367), Expect = 1.2e-262
Identity = 479/555 (86.31%), Postives = 504/555 (90.81%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSP---PYPISSITT---FWPRPNSFQIFPS 60
           M+TPVRFSSCRGVAFEVKPH+HHFSISKP+SP   P   S+ITT   FWPRPNSFQIFPS
Sbjct: 1   METPVRFSSCRGVAFEVKPHEHHFSISKPISPSSHPSASSNITTNTPFWPRPNSFQIFPS 60

Query: 61  PS-PSSLLRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHP 120
           PS PSSLLRSLS+PSSHFCDV   SDQ+++   +YD   LEEGD     TP   P  +HP
Sbjct: 61  PSAPSSLLRSLSRPSSHFCDV---SDQEQQ---EYD---LEEGDPH---TPVQNPKPSHP 120

Query: 121 --KNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNI 180
              NPKSRLSVILLDQGLFTVYKRLFLL LALNI AL+LAATGYFPYARA+P++FSIGNI
Sbjct: 121 PKNNPKSRLSVILLDQGLFTVYKRLFLLCLALNITALVLAATGYFPYARANPSVFSIGNI 180

Query: 181 FALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY 240
            AL++CRSEAFLRV+FWLAVT+ GR WVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY
Sbjct: 181 LALSVCRSEAFLRVVFWLAVTVLGRPWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVY 240

Query: 241 ALLLTLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALAL 300
           AL+LTL DP  RS  II VAS+ILALLCLSSLAAFPLVRHLHHNVFER HRFAGWTALAL
Sbjct: 241 ALVLTLTDPFNRSTPIIAVASSILALLCLSSLAAFPLVRHLHHNVFERIHRFAGWTALAL 300

Query: 301 LWAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPS 360
           LWAFLILTLTYDPITNSYNKD+ SRL  TQEFWFT AITFLIILPWVTVRRVPVQISAPS
Sbjct: 301 LWAFLILTLTYDPITNSYNKDVPSRLFQTQEFWFTAAITFLIILPWVTVRRVPVQISAPS 360

Query: 361 GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPP 420
           GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVS+PP
Sbjct: 361 GHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSNPP 420

Query: 421 SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENF 480
           SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADV+LVWVAKGIEENF
Sbjct: 421 SHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVYLVWVAKGIEENF 480

Query: 481 GKEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNA 540
           GKEIK MVNGYPKEKVIVHDTAVLGRPNVAEL+V AAG+WK EVVIVTSNPEGSRDVVNA
Sbjct: 481 GKEIKGMVNGYPKEKVIVHDTAVLGRPNVAELSVMAAGKWKTEVVIVTSNPEGSRDVVNA 540

Query: 541 CKSAGIAAFGPIWDS 547
           CKSAGIAAFGPIWDS
Sbjct: 541 CKSAGIAAFGPIWDS 543

BLAST of Lsi01G002530 vs. NCBI nr
Match: KAG6594557.1 (hypothetical protein SDJN03_11110, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032776.1 hypothetical protein SDJN02_06826, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 824.7 bits (2129), Expect = 4.7e-235
Identity = 434/554 (78.34%), Postives = 466/554 (84.12%), Query Frame = 0

Query: 1   MQTPVRFSSCRGVAFEVKPHDHHFSISKPMSPPYPISSITTFWPRPNSFQIFPSPSPSSL 60
           M TPVRFSSCRGVAFEVK  +H  S    +       S TTF             S SSL
Sbjct: 1   MATPVRFSSCRGVAFEVKAQEHQLSHQSSV-------SFTTF-----------LSSSSSL 60

Query: 61  LRSLSKPSSHFCDVD-------PLSDQDEEQHPQYDLQALEEGDQQNQSTPKNPPSQAHP 120
            RSLSKPS+HFCD+D       PLSD+DE      +LQ+LE+G          PP    P
Sbjct: 61  RRSLSKPSTHFCDIDPDQQKLQPLSDEDEN-----ELQSLEQGHY------VPPPQNPSP 120

Query: 121 KNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARASPALFSIGNIFA 180
            NPKSRLSVILLDQGLFTVYKRLF+LS++LNI ALILA T  FPYAR +PALFSIGNIF 
Sbjct: 121 PNPKSRLSVILLDQGLFTVYKRLFILSVSLNITALILALTRNFPYARNNPALFSIGNIFM 180

Query: 181 LTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCGVSSIAWLVYAL 240
           L LCRSEAFLRVLFWL+V+I GRSWVPL  KT++TSLLQSLGG+HSGCGVSSIAWLVYAL
Sbjct: 181 LCLCRSEAFLRVLFWLSVSIIGRSWVPLYVKTSLTSLLQSLGGIHSGCGVSSIAWLVYAL 240

Query: 241 LLTLM-DPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALL 300
           L+ ++ DP+  SPA+I VASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALL
Sbjct: 241 LIQILEDPKNASPAMIAVASAILALLCLSSLAAFPLVRHLHHNVFERTHRFAGWTALALL 300

Query: 301 WAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRRVPVQISAPSG 360
           WAF+IL LTYDPIT SY   L SRL   QEFWFT AITFLIILPWVTVRRVPVQISAPSG
Sbjct: 301 WAFIILKLTYDPITKSYTNHLGSRLVKNQEFWFTAAITFLIILPWVTVRRVPVQISAPSG 360

Query: 361 HASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDFTKSLVSDPPS 420
           HASIIKF+GGV+PGLLGRISPSPLSEWHAFGIISDG+KEHMMLAGAVGDFTKSLVS+PPS
Sbjct: 361 HASIIKFNGGVQPGLLGRISPSPLSEWHAFGIISDGQKEHMMLAGAVGDFTKSLVSNPPS 420

Query: 421 HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVWVAKGIEENFG 480
           HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFL+QRSRADV+LVWVAKGIEENFG
Sbjct: 421 HLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLIQRSRADVYLVWVAKGIEENFG 480

Query: 481 KEIKEMVNGYPKEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSNPEGSRDVVNAC 540
           KEIKEMVNGYPKEKVI+HDT V GRPNVAELTVKAAG+WKAEVVIVTSNPEGSRDVVNAC
Sbjct: 481 KEIKEMVNGYPKEKVIIHDTKVSGRPNVAELTVKAAGKWKAEVVIVTSNPEGSRDVVNAC 525

Query: 541 KSAGIAAFGPIWDS 547
           K +GIAAFGPIWDS
Sbjct: 541 KRSGIAAFGPIWDS 525

BLAST of Lsi01G002530 vs. TAIR 10
Match: AT4G18540.1 (unknown protein; Has 209 Blast hits to 205 proteins in 54 species: Archae - 0; Bacteria - 17; Metazoa - 2; Fungi - 150; Plants - 40; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 540.0 bits (1390), Expect = 2.1e-153
Identity = 304/565 (53.81%), Postives = 382/565 (67.61%), Query Frame = 0

Query: 5   VRFSSCRGVAFEVKPHDHHFSI---SKPMSPPYPISSITTFWPRPNSFQIFPSP-SPSSL 64
           VRFSSCRGVAFE+KP+ + F++    +  +        ++ +  P  F    S   PSS+
Sbjct: 7   VRFSSCRGVAFEIKPNANPFAVVTTDQNRNVNAEREGTSSRFRLPWDFMRNASKVVPSSI 66

Query: 65  LRSLSKPSSHFCDVDPLSDQDEEQHPQYDLQALEEG------DQQNQSTPK--------- 124
             S+S+ SSHFCD+DP  D D+E+  + D+  LEEG      ++ N++  K         
Sbjct: 67  EHSMSRASSHFCDLDP--DNDDEEEEKDDIFYLEEGGIKEGNEEHNETEEKAMLASSASK 126

Query: 125 ---NPPSQAHPKNPKSRLSVILLDQGLFTVYKRLFLLSLALNIAALILAATGYFPYARAS 184
               P     PK   SRLS+ILLDQGLFTVYK LF+LSL+LN+ AL+LAATG F YAR  
Sbjct: 127 HSEKPQQPPIPKKRPSRLSIILLDQGLFTVYKHLFVLSLSLNVLALVLAATGNFAYARNR 186

Query: 185 PALFSIGNIFALTLCRSEAFLRVLFWLAVTIFGRSWVPLAFKTAVTSLLQSLGGVHSGCG 244
            ALFSI NI AL LCRSEAFLR++F+L V + G S+VPL  K AVT+LLQSLGG+HSGCG
Sbjct: 187 AALFSIANILALILCRSEAFLRLVFYLTVKLLGHSFVPLRIKIAVTALLQSLGGIHSGCG 246

Query: 245 VSSIAWLVYALLLTLMDPQKRSPAIIVVASAILALLCLSSLAAFPLVRHLHHNVFERTHR 304
           VSS+AWL+YAL+LTL D    S AII VAS IL+LLCL+                     
Sbjct: 247 VSSVAWLIYALVLTLKDRDNTSTAIIAVASTILSLLCLT--------------------- 306

Query: 305 FAGWTALALLWAFLILTLTYDPITNSYNKDLASRLPHTQEFWFTTAITFLIILPWVTVRR 364
                   L+WAF+ILT++YDP + SY  DL S+L  TQEFWFT  IT  I+LPW+TVRR
Sbjct: 307 -------CLVWAFVILTVSYDPKSRSYTDDLGSKLIKTQEFWFTLVITIAILLPWLTVRR 366

Query: 365 VPVQISAPSGHASIIKFSGGVKPGLLGRISPSPLSEWHAFGIISDGEKEHMMLAGAVGDF 424
           VPV +S+ SGHAS+IKF GG+K G+LGRISPSPLSEWHAFGIISDG+  HMMLAGAVGDF
Sbjct: 367 VPVDVSSLSGHASLIKFRGGLKSGILGRISPSPLSEWHAFGIISDGKTSHMMLAGAVGDF 426

Query: 425 TKSLVSDPPSHLWVRGVHFAGLPYLVNMYERALVVATGSGICVFLSFLLQRSRADVHLVW 484
           TKSLVS PP+HLWVR VHFAGLPYLVN+Y++ L+V    G   F  FL  R         
Sbjct: 427 TKSLVSKPPTHLWVRTVHFAGLPYLVNLYDKVLLVPRVRGFAYFYRFLCNR--------- 486

Query: 485 VAKGIEENFGKEIKEMVNGYP-KEKVIVHDTAVLGRPNVAELTVKAAGEWKAEVVIVTSN 544
                       ++  +  YP ++++IVHDTA+LGRPNV++++V+A+ ++ A+VVIVTSN
Sbjct: 487 ------------VRLRIKDYPHQDRIIVHDTAILGRPNVSKMSVEASKKFGAQVVIVTSN 520

Query: 545 PEGSRDVVNACKSAGIAAFGPIWDS 547
           PEGSRDVVNACK++G+ AFGPIWDS
Sbjct: 547 PEGSRDVVNACKASGVPAFGPIWDS 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A8NVB73.1e-5634.31Adenylate-forming reductase 06235 OS=Coprinopsis cinerea (strain Okayama-7 / 130... [more]
A8NS279.0e-5634.47Adenylate-forming reductase 03009 OS=Coprinopsis cinerea (strain Okayama-7 / 130... [more]
Match NameE-valueIdentityDescription
A0A5A7UGJ43.5e-26888.02Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B0845.9e-26888.02uncharacterized protein LOC103484460 OS=Cucumis melo OX=3656 GN=LOC103484460 PE=... [more]
A0A0A0KI875.7e-26386.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G519460 PE=4 SV=1[more]
A0A6J1EEE62.5e-23477.98uncharacterized protein LOC111433565 OS=Cucurbita moschata OX=3662 GN=LOC1114335... [more]
A0A7J7C5Q77.8e-22070.94Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW21G01315 ... [more]
Match NameE-valueIdentityDescription
XP_038883706.12.7e-29193.41adenylate-forming reductase 03009 [Benincasa hispida][more]
KAA0052631.17.1e-26888.02uncharacterized protein E6C27_scaffold120G002410 [Cucumis melo var. makuwa] >TYK... [more]
XP_008439758.11.2e-26788.02PREDICTED: uncharacterized protein LOC103484460 [Cucumis melo][more]
XP_011658244.11.2e-26286.31adenylate-forming reductase 06235 [Cucumis sativus] >KGN49293.1 hypothetical pro... [more]
KAG6594557.14.7e-23578.34hypothetical protein SDJN03_11110, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT4G18540.12.1e-15353.81unknown protein; Has 209 Blast hits to 205 proteins in 54 species: Archae - 0; B... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..116
NoneNo IPR availablePANTHERPTHR33927TRANSMEMBRANE PROTEINcoord: 8..546
NoneNo IPR availablePANTHERPTHR33927:SF7SUBFAMILY NOT NAMEDcoord: 8..546
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 428..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G002530.1Lsi01G002530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane