Lsi01G001930 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G001930
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr01: 1851537 .. 1853279 (+)
RNA-Seq Expression	Lsi01G001930
Synteny	Lsi01G001930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGGTATTTTATCTACTTGGCTATCTTTATATTAGCGAATAAGTAAATGGATTAAAAGGCTACAAATTCAATGAAACTAGTATATATTATATCACAAATCATACACATGCAAATTACTCTATATTAAGGAATAAGAATTAGTCGTTTTACAACTACTCATCGAGCTATCAAATCACAGTTTGTCACATTAATGACATATTCTTTTTTTTTTCTTTTTCTTAAATGTATTTTTAGACAAAAAAAAAAAAAAATGGATACAATGGAGGATAAATTCAAGCAGTGATTTTTGAGAGTGTATAAATAGATGGGTTGTATGGTGTGTGGTAATGCCATAACTCAAAGAGACTTTGAAACTAAAGGCCATTTACAAAGAAAAAGAAAAGATGATTAGCGTAAAAGAAGTAGCACAAAAGAGCTCGAAAATCACAATCCCACAAAACTTCATTCGTTTGGATCAAGAACCTTCTTCTACCTGTGATCCTTCAACCTTCCCCACCCCTCCAATCATTGATATGAGCCGATTACTCTCACCAAAACATTCTCAATCCGAGCTTCTCAATCTTCACTCGGCTTGCAAAGGATGGGGATTGTTTCAGGTAACTCAGATCGTAACTATATATTTCTATAATGATATGATATTATTGTTTGAGCTAAGTTTGAGGTATATAATTTTAGTTGGTGAACCATGGGGTTAGCTGTTCATTGTTGGGAGAGCTAAAGCACGAGATTGAAGAATTTTTTGAGATTCCATTTGAAGAGAAGATGAAGTTTGGTATGAAGAGTGGAGAAGTTGAGGGTTATGGAACAGTGGTACGAAGCATGGAACAGAAACTTGATTGGGGTGATAGATTTTACATCATTACTAATCCTCTTTCAAGAAGGAAACCCCATCTTCTTCCTCACCTCCCTTCATCATTAAGGTCCTACTTCTTTCTCTCTCTAGCCCCACTCTATTTGGTTTTCGAACATTAAGTTTATAAACATTATCTCTATCTACAAATTTTCGTATTTTGTTATCTATTTTTTATCCATATTTTTAAAAAAGAAAAAATCAAACTAAGTTTTGAAAACTATAATAGAGAAATTGGGAGGAAATAAACATAAATTTAAAAAATCAAACACTATCAATTGGAGCTTCTAATAACACTACTTCTACCTATTGGTTTCATTGTTTTTTTTTATATAATTTTTTCTTTAAAAATCTAAGTTAAGTTTTGAAAACTAAAAGGAATTCAAAGTTTTTTCTTTTTTCTTTTTGCTTTTTAAAATTTAGCTAATAATTCAATGGTTTATTTTAAAAAGATGAAAACTATTAAAAGAATGATATGAAAATAAACACAATTTTCAAAGACTAAAGCCCTATTGATAACTAAATTATTTTTTGTTTTTGGTTTTTAAAAATAAGTTTATAAATACTACTTCTACTCCTAAGTTGATGTGTTTTGCTATTCACTTTCTTATGGTGTTTCTAACAACAAAAAAACAGTATGATTTTCGAAAATTTATTTTTGTTTATAGAATGTGAAAACCATTGTCAAAAAATTGAAATGGGAAACAAGCATAACTTGGAAAAAAAACAAAATAAAAACAAAAAAAAAAATGTAATCGTTACTTATGTATGGTTGCAGAAATGCATTGGAATCGTATTTGAAAGAGACGAAAAAGATAACGATGGCGTTGTTGAGGATGATAGCGGGAAATTTGGAGATAGAGGTGAGGAAGATGGAGGAGTTGTTTGA

mRNA sequence

ATGATAGCACAAAAGAGCTCGAAAATCACAATCCCACAAAACTTCATTCGTTTGGATCAAGAACCTTCTTCTACCTGTGATCCTTCAACCTTCCCCACCCCTCCAATCATTGATATGAGCCGATTACTCTCACCAAAACATTCTCAATCCGAGCTTCTCAATCTTCACTCGGCTTGCAAAGGATGGGGATTGTTTCAGTTGGTGAACCATGGGGTTAGCTGTTCATTGTTGGGAGAGCTAAAGCACGAGATTGAAGAATTTTTTGAGATTCCATTTGAAGAGAAGATGAAGTTTGGTATGAAGAGTGGAGAAGTTGAGGGTTATGGAACAGTGAAATGCATTGGAATCGTATTTGAAAGAGACGAAAAAGATAACGATGGCGTTGTTGAGGATGATAGCGGGAAATTTGGAGATAGAGGTGAGGAAGATGGAGGAGTTGTTTGA

Coding sequence (CDS)

ATGATAGCACAAAAGAGCTCGAAAATCACAATCCCACAAAACTTCATTCGTTTGGATCAAGAACCTTCTTCTACCTGTGATCCTTCAACCTTCCCCACCCCTCCAATCATTGATATGAGCCGATTACTCTCACCAAAACATTCTCAATCCGAGCTTCTCAATCTTCACTCGGCTTGCAAAGGATGGGGATTGTTTCAGTTGGTGAACCATGGGGTTAGCTGTTCATTGTTGGGAGAGCTAAAGCACGAGATTGAAGAATTTTTTGAGATTCCATTTGAAGAGAAGATGAAGTTTGGTATGAAGAGTGGAGAAGTTGAGGGTTATGGAACAGTGAAATGCATTGGAATCGTATTTGAAAGAGACGAAAAAGATAACGATGGCGTTGTTGAGGATGATAGCGGGAAATTTGGAGATAGAGGTGAGGAAGATGGAGGAGTTGTTTGA

Protein sequence

MIAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKGWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTVKCIGIVFERDEKDNDGVVEDDSGKFGDRGEEDGGVV

Homology

[Close]

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.5e-18
Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 10  TIPQNFIRLDQEPSSTCDPSTFPTP-PIIDMSRLLSPKHSQSELLNLHSACKGWGLFQLV 69
           T+P  ++R DQ+ +   D        PIIDM RL S     SE+  L  ACK WG FQLV
Sbjct: 28  TVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLV 87

Query: 70  NHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
           NHG+  S L ++K EI++FF +P EEK KF  +  E+EG+G
Sbjct: 88  NHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFG 128

[Close]

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.5e-17
Identity = 48/113 (42.48%), Postives = 66/113 (58.41%), Query Frame = 0


Query: 4   QKSSKIT---IPQNFIRLDQEPSSTCDPSTF--PTPPIIDMSRLLSPKH--SQSELLNLH 63
           Q+ +K+T   IP  +    + P +    S     T P+ID+  LLSP+    + EL  LH
Sbjct: 19  QELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVVGKLELDKLH 78

Query: 64  SACKGWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
           SACK WG FQLVNHGV   L+  +K EI+ FF +P  EK K+G + G+ EG+G
Sbjct: 79  SACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEGFG 131

[Close]

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.2e-16
Identity = 40/79 (50.63%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 33  TPPIIDMSRLLS--PKHSQSELLNLHSACKGWGLFQLVNHGVSCSLLGELKHEIEEFFEI 92
           T P+ID+  L+S  P   + EL  LHSACK WG FQ+VNHGV  SL+  +K +I+ FF +
Sbjct: 54  TVPVIDIENLISSEPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNL 113

Query: 93  PFEEKMKFGMKSGEVEGYG 110
              EK+K+G K G+VEG+G
Sbjct: 114 SMNEKIKYGQKDGDVEGFG 132

[Close]

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.1e-15
Identity = 45/114 (39.47%), Postives = 67/114 (58.77%), Query Frame = 0


Query: 4   QKSSKIT---IPQNFIRLDQE---PSSTCDPSTFPTPPIIDMSRLLSPKH--SQSELLNL 63
           Q+ +K+T   IP  ++  ++    P      +   T P+ID+  LLSP+    + EL  L
Sbjct: 19  QELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSPEPIIGKLELDRL 78

Query: 64  HSACKGWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
           H ACK WG FQ+VNHGV  SL+  +K EI+ FF +  +EK K+  + G+VEG+G
Sbjct: 79  HFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDGDVEGFG 132

[Close]

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.0e-14
Identity = 40/109 (36.70%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 1   MIAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACK 60
           ++A K  K  +P  ++R + E             P+ID+SRLL  +++  EL   HSAC 
Sbjct: 19  VLAGKELK-NLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACDELAKFHSACL 78

Query: 61  GWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
            WG FQL+NHGV   ++ ++K + E+FF +PF+EK  +      +EGYG
Sbjct: 79  DWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYG 126

[Close]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL
Match: A0A5A7UA06 (Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001880 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 5.3e-46
Identity = 92/110 (83.64%), Postives = 102/110 (92.73%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KSSK+TIPQNF+RLDQE SST DPSTFPTPPIIDMSRLLSP+HS+SELLNLHSAC  
Sbjct: 7   LVEKSSKLTIPQNFLRLDQEASSTSDPSTFPTPPIIDMSRLLSPQHSRSELLNLHSACIQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLLGELKHEIE FFE+P +EKMK+GM+SGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSLSLLGELKHEIEGFFELPLKEKMKYGMRSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL
Match: A0A5D3CPF6 (Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008590 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 5.3e-46
Identity = 92/110 (83.64%), Postives = 102/110 (92.73%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KSSK+TIPQNF+RLDQE SST DPSTFPTPPIIDMSRLLSP+HS+SELLNLHSAC  
Sbjct: 7   LVEKSSKLTIPQNFLRLDQEASSTSDPSTFPTPPIIDMSRLLSPQHSRSELLNLHSACIQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLLGELKHEIE FFE+P +EKMK+GM+SGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSLSLLGELKHEIEGFFELPLKEKMKYGMRSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL
Match: A0A6J1INU8 (codeine O-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111478599 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 5.5e-43
Identity = 88/110 (80.00%), Postives = 97/110 (88.18%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + + SSKI+IPQNFIRLDQEPSST  PSTFP PP IDMSRLLSP+HS+SELLNLHSACK 
Sbjct: 7   LVESSSKISIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSPQHSRSELLNLHSACKQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVSCSLL E+K EIE FFE+P +EKMKF MK+GE EGYGTV
Sbjct: 67  WGLFQLVNHGVSCSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL
Match: A0A6J1ED48 (codeine O-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111433087 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.0e-41
Identity = 87/110 (79.09%), Postives = 95/110 (86.36%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + + SSKI IPQNFIRLDQEPSST  PSTFP PP IDMSRLLSP+HS+SELLNLHSACK 
Sbjct: 7   LVESSSKINIPQNFIRLDQEPSSTSHPSTFPAPPTIDMSRLLSPQHSRSELLNLHSACKQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLL E+K EIE FFE+P +EKMKF MK+GE EGYGTV
Sbjct: 67  WGLFQLVNHGVSSSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL
Match: A0A6J1CKJ4 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012449 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.6e-34
Identity = 80/122 (65.57%), Postives = 96/122 (78.69%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           +A+KSSK  IP+NFIRLDQEP S+ DP +FP PP IDM++LLS  HS+SEL  LHSAC+ 
Sbjct: 1   MAKKSSK--IPENFIRLDQEPCSSSDPGSFPVPPTIDMNQLLS--HSESELQKLHSACQQ 60

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTVKCIGIVFERD 121
           WGLFQLVNHGV   LL E+K EIEEFFE+P EEKMKFG+K GEVEGYG+     +V  +D
Sbjct: 61  WGLFQLVNHGVRRCLLEEVKREIEEFFEVPLEEKMKFGIKGGEVEGYGS-----MVLSQD 113

Query: 122 EK 124
           +K
Sbjct: 121 QK 113

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BLAST of Lsi01G001930 vs. NCBI nr
Match: XP_038883393.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 200.7 bits (509), Expect = 8.9e-48
Identity = 96/110 (87.27%), Postives = 103/110 (93.64%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KSS ITIPQNFIRLDQEPSST DPSTFPTPP IDM+RLLS +HS+SELLNLHSACK 
Sbjct: 7   LVEKSSIITIPQNFIRLDQEPSSTSDPSTFPTPPTIDMTRLLSLQHSRSELLNLHSACKQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVSCSLLGELKHEIEEFFE+P EEKMK+GMKSGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSCSLLGELKHEIEEFFELPLEEKMKYGMKSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. NCBI nr
Match: KAA0052582.1 (codeine O-demethylase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 193.7 bits (491), Expect = 1.1e-45
Identity = 92/110 (83.64%), Postives = 102/110 (92.73%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KSSK+TIPQNF+RLDQE SST DPSTFPTPPIIDMSRLLSP+HS+SELLNLHSAC  
Sbjct: 7   LVEKSSKLTIPQNFLRLDQEASSTSDPSTFPTPPIIDMSRLLSPQHSRSELLNLHSACIQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLLGELKHEIE FFE+P +EKMK+GM+SGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSLSLLGELKHEIEGFFELPLKEKMKYGMRSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. NCBI nr
Match: TYK13242.1 (codeine O-demethylase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 193.7 bits (491), Expect = 1.1e-45
Identity = 92/110 (83.64%), Postives = 102/110 (92.73%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KSSK+TIPQNF+RLDQE SST DPSTFPTPPIIDMSRLLSP+HS+SELLNLHSAC  
Sbjct: 7   LVEKSSKLTIPQNFLRLDQEASSTSDPSTFPTPPIIDMSRLLSPQHSRSELLNLHSACIQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLLGELKHEIE FFE+P +EKMK+GM+SGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSLSLLGELKHEIEGFFELPLKEKMKYGMRSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. NCBI nr
Match: XP_004134920.1 (codeine O-demethylase [Cucumis sativus] >KAE8647732.1 hypothetical protein Csa_003989 [Cucumis sativus])

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-44
Identity = 90/110 (81.82%), Postives = 101/110 (91.82%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + +KS+KITIPQNFIRLDQE S+T DPSTFPTPPIIDMSRLLSP++S+SELL LHSAC  
Sbjct: 7   LVEKSTKITIPQNFIRLDQEASTTSDPSTFPTPPIIDMSRLLSPQYSRSELLKLHSACIE 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVS SLLGELKHE+E FF++P EEKMK+GMKSGEVEGYGTV
Sbjct: 67  WGLFQLVNHGVSFSLLGELKHEVEGFFDLPLEEKMKYGMKSGEVEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. NCBI nr
Match: XP_022978731.1 (codeine O-demethylase-like [Cucurbita maxima])

HSP 1 Score: 183.7 bits (465), Expect = 1.1e-42
Identity = 88/110 (80.00%), Postives = 97/110 (88.18%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKG 61
           + + SSKI+IPQNFIRLDQEPSST  PSTFP PP IDMSRLLSP+HS+SELLNLHSACK 
Sbjct: 7   LVESSSKISIPQNFIRLDQEPSSTSHPSTFPVPPTIDMSRLLSPQHSRSELLNLHSACKQ 66

Query: 62  WGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           WGLFQLVNHGVSCSLL E+K EIE FFE+P +EKMKF MK+GE EGYGTV
Sbjct: 67  WGLFQLVNHGVSCSLLEEVKSEIEGFFELPLDEKMKFCMKAGEFEGYGTV 116

[Close]

BLAST of Lsi01G001930 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 4.0e-22
Identity = 49/114 (42.98%), Postives = 72/114 (63.16%), Query Frame = 0


Query: 2   IAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTP-PIIDMSRLLSPKHSQSELLNLHSACK 61
           I ++ +  TIP  ++R+DQE +   + S+  +  P+IDM+RL S     SEL  L  AC+
Sbjct: 20  IVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDSELKKLDFACQ 79

Query: 62  GWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTVKCI 115
            WG FQLVNHG+  S L +L+ E++EFF +P +EK K   +SGE EG+G V  +
Sbjct: 80  DWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQVNIV 133

[Close]

BLAST of Lsi01G001930 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-19
Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 10  TIPQNFIRLDQEPSSTCDPSTFPTP-PIIDMSRLLSPKHSQSELLNLHSACKGWGLFQLV 69
           T+P  ++R DQ+ +   D        PIIDM RL S     SE+  L  ACK WG FQLV
Sbjct: 28  TVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLV 87

Query: 70  NHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
           NHG+  S L ++K EI++FF +P EEK KF  +  E+EG+G
Sbjct: 88  NHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFG 128

[Close]

BLAST of Lsi01G001930 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.2e-18
Identity = 45/103 (43.69%), Postives = 61/103 (59.22%), Query Frame = 0


Query: 10  TIPQNFIRLDQEPSS-TCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKGWGLFQLV 69
           T+P  ++R DQ+ +    D       PIIDMS L S     SE+  L SACK WG FQLV
Sbjct: 27  TVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDSEIDKLDSACKEWGFFQLV 86

Query: 70  NHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTV 112
           NHG+  S L ++K E+++FF +P EEK     +  E+EG+G V
Sbjct: 87  NHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQV 129

[Close]

BLAST of Lsi01G001930 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-17
Identity = 48/123 (39.02%), Postives = 66/123 (53.66%), Query Frame = 0


Query: 1   MIAQKSSKITIPQNFIRLDQEPSSTCDPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACK 60
           M+ +K     +P  ++R DQE       S     PIIDMS L S     SE+  L  ACK
Sbjct: 19  MVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDSEIDKLDFACK 78

Query: 61  GWGLFQLVNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYGTVKCIGIVFER 120
            WG FQLVNHG+    L + K +I++FF +P EEK K   + G++EG+G       VF  
Sbjct: 79  EWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQ----AFVFSE 134

Query: 121 DEK 124
           ++K
Sbjct: 139 EQK 134

[Close]

BLAST of Lsi01G001930 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 6.6e-17
Identity = 43/102 (42.16%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 10  TIPQNFIRLDQEPSSTC--DPSTFPTPPIIDMSRLLSPKHSQSELLNLHSACKGWGLFQL 69
           T+P  ++R DQ+ +     D       PIIDM+RL S     SE+  L  ACK +G FQL
Sbjct: 28  TVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVDSEVEKLDFACKEYGFFQL 87

Query: 70  VNHGVSCSLLGELKHEIEEFFEIPFEEKMKFGMKSGEVEGYG 110
           VNHG+  S L ++K EI++FF +P EEK K       +EG+G
Sbjct: 88  VNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFG 129

The following BLAST results are available for this feature:

BLAST of Lsi01G001930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39224	1.5e-18	45.54	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N502	6.5e-17	42.48	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
D4N501	4.2e-16	50.63	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
D4N500	6.1e-15	39.47	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
A2A1A0	1.0e-14	36.70	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]

BLAST of Lsi01G001930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UA06	5.3e-46	83.64	Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A5D3CPF6	5.3e-46	83.64	Codeine O-demethylase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1INU8	5.5e-43	80.00	codeine O-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111478599 PE=3 SV=1	[more]
A0A6J1ED48	1.0e-41	79.09	codeine O-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111433087 PE=3 SV...	[more]
A0A6J1CKJ4	1.6e-34	65.57	protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012449 PE=3 SV=1	[more]

BLAST of Lsi01G001930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883393.1	8.9e-48	87.27	protein SRG1-like [Benincasa hispida]	[more]
KAA0052582.1	1.1e-45	83.64	codeine O-demethylase-like isoform X1 [Cucumis melo var. makuwa]	[more]
TYK13242.1	1.1e-45	83.64	codeine O-demethylase-like isoform X1 [Cucumis melo var. makuwa]	[more]
XP_004134920.1	2.7e-44	81.82	codeine O-demethylase [Cucumis sativus] >KAE8647732.1 hypothetical protein Csa_0...	[more]
XP_022978731.1	1.1e-42	80.00	codeine O-demethylase-like [Cucurbita maxima]	[more]

BLAST of Lsi01G001930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78550.1	4.0e-22	42.98	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	1.1e-19	45.54	senescence-related gene 1	[more]
AT4G25300.1	1.2e-18	43.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25310.1	1.3e-17	39.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17010.1	6.6e-17	42.16	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..139 e-value: 1.6E-22 score: 82.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 35..111 e-value: 1.3E-20 score: 74.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..147
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 6..110
None	No IPR available	PANTHER	PTHR47991:SF58	CODEINE O-DEMETHYLASE-LIKE ISOFORM X1	coord: 6..110
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G001930.1	Lsi01G001930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016491	oxidoreductase activity