Lsi01G001400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G001400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description9-cis-epoxycarotenoid dioxygenase
Locationchr01: 1451654 .. 1453447 (-)
RNA-Seq ExpressionLsi01G001400
SyntenyLsi01G001400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGCTTCTTCTTCTTTACTTGATCATTTGGGGTTTGTTAATTCCATTCCCAAATCAAACTTCCATAGTTTCAGTTACAATAATGTTAAGATCATTAAGCAAAACTCATCCCACAATACCATTATTCAATCTGCTCTTCACTCTCCTTCTGTTCTTCATTTCCCAAATCAAACTCCTAATTTCCACACCAATCTCACTCTCAAAGATCAAACCAACAAATCCCCTTTTTCCAATTGGAATTTCCTTCAGAAAACAGCTTCCTTCGCCTTAGACATGGTGGAATCCGCTTTAGTTTCTCACGAGCTTCAACATCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCTGGCAATTTCGCTCCCGTCCCAGAACAACCTGTTAAACATAACCTCCCCGTTATCGGAACCATCCCGGATTGCATTAACGGCGTTTACCTTCGGAACGGCGCCAATCCTCTCTTTGAGCCAACTGCCGGACACCATCTCTTCGATGGCGATGGAATGGTTCACGCTGTGACCATCAATGACGGCACCGCCAGCTACGCTTGCCGGTTCACTCAGACCCAAAGGCTGGTTCAGGAACGGCGACTGGGGAGACCAGTTTTTCCTAAAGCGATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGGCTGTTACTGTTCTACGCGCGAGGAGTGTTTGGGCTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGCGTATTTCAATGACCGGCTTTTGGCTATGTCAGAAGACGATTTGCCGTACCACGTGCGGATCACGCCCTCAGGTGACCTACAAACCGTAGGCCGTTACGATTTCAACAAACAGCTTGATTCTACAATGATCGCACATCCGAAAGTGGACCCAGTTTCCAGAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAGCCGTATCTCAAATACTTCCGATTCTCCCCCGACGGAACCAAATCCAAAGACGTTGAAATCCCTGTTGAAACTCCCACAATGATGCACGACTTCGCCATCACAGAGAATTTCGTAGTCATTCCCGACCAGCAAGTCGTCTTCAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACTGATTCGAAGGATTTGATCTGGGTGGAATCCCCGGATACGTTCTGTTTCCATCTGTGGAATGCTTGGGAAGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATATTCAACGAGTGTGAGGAGAATTTGAAAAGCGTTTTATCGGAAATTCGGCTGAATTTAAGGACGGGGAAATCGACACGGCGGGCAATTATCAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAACAGAAACCGCCTGGGGCGGAAAAGCCGATATGCGTATTTGGCAATTGCAGAACCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTCGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAGGGGAAGAAGAAGAAGAAGATGAAGGGTACATTATGGCGTTTGTTCATGATGAGAGGACAGGGAAATCAGAGGTGCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATCAAATTGCCTTCTCGAGTGCCCTATGGATTTCATGGGACATTTGTCCACGCAAACAATTTGCAAAATCAAGCATAA

mRNA sequence

ATGACGGCTTCTTCTTCTTTACTTGATCATTTGGGGTTTGTTAATTCCATTCCCAAATCAAACTTCCATAGTTTCAGTTACAATAATGTTAAGATCATTAAGCAAAACTCATCCCACAATACCATTATTCAATCTGCTCTTCACTCTCCTTCTGTTCTTCATTTCCCAAATCAAACTCCTAATTTCCACACCAATCTCACTCTCAAAGATCAAACCAACAAATCCCCTTTTTCCAATTGGAATTTCCTTCAGAAAACAGCTTCCTTCGCCTTAGACATGGTGGAATCCGCTTTAGTTTCTCACGAGCTTCAACATCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCTGGCAATTTCGCTCCCGTCCCAGAACAACCTGTTAAACATAACCTCCCCGTTATCGGAACCATCCCGGATTGCATTAACGGCGTTTACCTTCGGAACGGCGCCAATCCTCTCTTTGAGCCAACTGCCGGACACCATCTCTTCGATGGCGATGGAATGGTTCACGCTGTGACCATCAATGACGGCACCGCCAGCTACGCTTGCCGGTTCACTCAGACCCAAAGGCTGGTTCAGGAACGGCGACTGGGGAGACCAGTTTTTCCTAAAGCGATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGGCTGTTACTGTTCTACGCGCGAGGAGTGTTTGGGCTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGCGTATTTCAATGACCGGCTTTTGGCTATGTCAGAAGACGATTTGCCGTACCACGTGCGGATCACGCCCTCAGGTGACCTACAAACCGTAGGCCGTTACGATTTCAACAAACAGCTTGATTCTACAATGATCGCACATCCGAAAGTGGACCCAGTTTCCAGAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAGCCGTATCTCAAATACTTCCGATTCTCCCCCGACGGAACCAAATCCAAAGACGTTGAAATCCCTGTTGAAACTCCCACAATGATGCACGACTTCGCCATCACAGAGAATTTCGTAGTCATTCCCGACCAGCAAGTCGTCTTCAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACTGATTCGAAGGATTTGATCTGGGTGGAATCCCCGGATACGTTCTGTTTCCATCTGTGGAATGCTTGGGAAGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATATTCAACGAGTGTGAGGAGAATTTGAAAAGCGTTTTATCGGAAATTCGGCTGAATTTAAGGACGGGGAAATCGACACGGCGGGCAATTATCAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAACAGAAACCGCCTGGGGCGGAAAAGCCGATATGCGTATTTGGCAATTGCAGAACCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTCGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAGGGGAAGAAGAAGAAGAAGATGAAGGGTACATTATGGCGTTTGTTCATGATGAGAGGACAGGGAAATCAGAGGTGCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATCAAATTGCCTTCTCGAGTGCCCTATGGATTTCATGGGACATTTGTCCACGCAAACAATTTGCAAAATCAAGCATAA

Coding sequence (CDS)

ATGACGGCTTCTTCTTCTTTACTTGATCATTTGGGGTTTGTTAATTCCATTCCCAAATCAAACTTCCATAGTTTCAGTTACAATAATGTTAAGATCATTAAGCAAAACTCATCCCACAATACCATTATTCAATCTGCTCTTCACTCTCCTTCTGTTCTTCATTTCCCAAATCAAACTCCTAATTTCCACACCAATCTCACTCTCAAAGATCAAACCAACAAATCCCCTTTTTCCAATTGGAATTTCCTTCAGAAAACAGCTTCCTTCGCCTTAGACATGGTGGAATCCGCTTTAGTTTCTCACGAGCTTCAACATCCACTCCCTAAAACCGCCGACCCACGTGTCCAAATCTCTGGCAATTTCGCTCCCGTCCCAGAACAACCTGTTAAACATAACCTCCCCGTTATCGGAACCATCCCGGATTGCATTAACGGCGTTTACCTTCGGAACGGCGCCAATCCTCTCTTTGAGCCAACTGCCGGACACCATCTCTTCGATGGCGATGGAATGGTTCACGCTGTGACCATCAATGACGGCACCGCCAGCTACGCTTGCCGGTTCACTCAGACCCAAAGGCTGGTTCAGGAACGGCGACTGGGGAGACCAGTTTTTCCTAAAGCGATTGGGGAACTCCATGGCCACTCCGGAATCGCTCGGCTGTTACTGTTCTACGCGCGAGGAGTGTTTGGGCTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGCGTATTTCAATGACCGGCTTTTGGCTATGTCAGAAGACGATTTGCCGTACCACGTGCGGATCACGCCCTCAGGTGACCTACAAACCGTAGGCCGTTACGATTTCAACAAACAGCTTGATTCTACAATGATCGCACATCCGAAAGTGGACCCAGTTTCCAGAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAGCCGTATCTCAAATACTTCCGATTCTCCCCCGACGGAACCAAATCCAAAGACGTTGAAATCCCTGTTGAAACTCCCACAATGATGCACGACTTCGCCATCACAGAGAATTTCGTAGTCATTCCCGACCAGCAAGTCGTCTTCAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAACAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACTGATTCGAAGGATTTGATCTGGGTGGAATCCCCGGATACGTTCTGTTTCCATCTGTGGAATGCTTGGGAAGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATATTCAACGAGTGTGAGGAGAATTTGAAAAGCGTTTTATCGGAAATTCGGCTGAATTTAAGGACGGGGAAATCGACACGGCGGGCAATTATCAAGGAAGAGAAAGAACAGGTGAATCTAGAAGCGGGAATGGTGAACAGAAACCGCCTGGGGCGGAAAAGCCGATATGCGTATTTGGCAATTGCAGAACCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTCGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGAGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAGGGGAAGAAGAAGAAGAAGATGAAGGGTACATTATGGCGTTTGTTCATGATGAGAGGACAGGGAAATCAGAGGTGCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATCAAATTGCCTTCTCGAGTGCCCTATGGATTTCATGGGACATTTGTCCACGCAAACAATTTGCAAAATCAAGCATAA

Protein sequence

MTASSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA
Homology
[Close]
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 891.0 bits (2301), Expect = 7.6e-258
Identity = 430/591 (72.76%), Postives = 501/591 (84.77%), Query Frame = 0 

Query: 16  SIPKSNFHSFSYNNVKIIK-QNSSHNTIIQSALHSPSVLHFPNQTPNFHT--NLTL---- 75
           S+P S    F+ N++ ++K Q++  +  I S+L +P +LHFP Q+ N+ T  N T+    
Sbjct: 18  SMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPK 77

Query: 76  --KDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPE 135
              + ++ S  S WN +QK A+ ALD VESAL  HEL+HPLPKTADPRVQISGNFAPVPE
Sbjct: 78  QENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPE 137

Query: 136 QPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACR 195
            PV  +LPV G IP C+ GVY+RNGANPLFEPTAGHH FDGDGMVHAV   +G+ASYACR
Sbjct: 138 NPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACR 197

Query: 196 FTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLA 255
           FT+T+RLVQE+ LGRPVFPKAIGELHGHSGIARL+LFYARG+FGLVDH KGTGVANAGL 
Sbjct: 198 FTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLV 257

Query: 256 YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSY 315
           YFN+RLLAMSEDDLPYHV++TP+GDL+T GR+DF+ QL STMIAHPK+DPVS EL+ALSY
Sbjct: 258 YFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSY 317

Query: 316 DVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKG 375
           DV++KPYLKYFRFS +G KS DVEIPVE PTMMHDFAITENFVVIPDQQVVFK+ EM++G
Sbjct: 318 DVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRG 377

Query: 376 GSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMT 435
           GSPV+YDKNK SRFGIL K A D  DL WVE PD FCFHLWNAWEE ET+E+VVIGSCMT
Sbjct: 378 GSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMT 437

Query: 436 PPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRY 495
           PPDSIFNEC+E LKSVLSEIRLNL+TGKSTR++II+   EQVNLEAGMVNRN+LGRK+ Y
Sbjct: 438 PPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEY 497

Query: 496 AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIM 555
           AYLAIAEPWPKVSGFAKV+L TGEV+K IYG  K+GGEP FLP++ NS   +EED+GYI+
Sbjct: 498 AYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNS---KEEDDGYIL 557

Query: 556 AFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           AFVHDE+  KSE+QIVNAM LK+EAT+KLPSRVPYGFHGTF++AN+L NQA
Sbjct: 558 AFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

[Close]
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 4.2e-256
Identity = 427/565 (75.58%), Postives = 493/565 (87.26%), Query Frame = 0 

Query: 33  IKQNSSHNTIIQSALHS-PSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFLQKTASFAL 92
           +K +++  T I SAL + P   + P   P F T             S+WNF QK A+ AL
Sbjct: 33  MKNHTTTKTKIHSALLTLPKQNNTPKNQPQFQT-------------SHWNFFQKAAAKAL 92

Query: 93  DMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNG 152
           D+VESALVS ELQ+PLPKTADPRVQI+GNFAPVPEQ V+HNLPV GTIPDCINGVY+RNG
Sbjct: 93  DIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYVRNG 152

Query: 153 ANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGEL 212
           ANPLFEP AGHHLFDGDGMVHAVT+ +G+ SY+CRFT+T+RLVQER LG PVFPKAIGEL
Sbjct: 153 ANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERLVQERELGHPVFPKAIGEL 212

Query: 213 HGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGD 272
           HGHSGIARLLLFYARGVFGLVDH  GTGVANAGL +FN+RLLAMSEDD+PYHV++ PSGD
Sbjct: 213 HGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGD 272

Query: 273 LQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEI 332
           LQTVGRY+F+ QL STMIAHPK+DPVS EL+ALSYDVV+KPYLK F+FSPDG KS DVEI
Sbjct: 273 LQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSPDVEI 332

Query: 333 PVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSK 392
           P++ PTMMHDFAITEN+VVIPDQQVVFKLQEM+KGGSPVIYDKNKKSRFGILPKNA +S+
Sbjct: 333 PLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNAENSE 392

Query: 393 DLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLR 452
           ++IWVES +TFCFHLWNAWEEPET+EV+VIGSCMTPPDSIFNEC ENLKSVLSEIRLNL+
Sbjct: 393 NIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIRLNLK 452

Query: 453 TGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEV 512
           TG+STRR ++    +QVNLEAGMVNRN+LGRK+++AYLAIAEPWPKVSGFAKVDL TGE+
Sbjct: 453 TGESTRRQLL-SPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEI 512

Query: 513 KKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEA 572
           KKHIYG +++GGEP FLP+  NS   E+ED+GYI+AF HDE+T KSE+QIVNAM L++EA
Sbjct: 513 KKHIYGDKRYGGEPLFLPRNVNS---EKEDDGYILAFCHDEKTWKSELQIVNAMTLELEA 572

Query: 573 TIKLPSRVPYGFHGTFVHANNLQNQ 597
           T+KLPSRVPYGFHGTF+ + +LQNQ
Sbjct: 573 TVKLPSRVPYGFHGTFISSKDLQNQ 580

[Close]
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.1e-243
Identity = 404/554 (72.92%), Postives = 463/554 (83.57%), Query Frame = 0 

Query: 43  IQSALHSPSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHE 102
           + SALH+P  LHFP Q+ N    + +K +  +S     N  Q+ A+ ALD  E  LVSHE
Sbjct: 50  VSSALHTPPALHFPKQSSN-SPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHE 109

Query: 103 LQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGH 162
             HPLPKTADP VQI+GNFAPV EQPV+ NLPV+G +PD I GVY+RNGANPL EP  GH
Sbjct: 110 KLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGH 169

Query: 163 HLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLL 222
           H FDGDGMVHAV    G+ASYACRFTQT R VQER+LGRPVFPKAIGELHGH+GIARL+L
Sbjct: 170 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 229

Query: 223 FYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNK 282
           FYAR   G+VD   GTGVANAGL YFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ 
Sbjct: 230 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDG 289

Query: 283 QLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDF 342
           QL+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDGTKS DVEI ++ PTMMHDF
Sbjct: 290 QLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDF 349

Query: 343 AITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTF 402
           AITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A DS ++ W+++PD F
Sbjct: 350 AITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCF 409

Query: 403 CFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIK 462
           CFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG+STRR II 
Sbjct: 410 CFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 469

Query: 463 EEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFG 522
            E +QVNLEAGMVNRN LGRK+++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++G
Sbjct: 470 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYG 529

Query: 523 GEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYG 582
           GEP FLP EG      EEDEGYI+ FVHDE+T KSE+QIVNA+ L+VEAT+KLPSRVPYG
Sbjct: 530 GEPLFLPGEGG-----EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYG 589

Query: 583 FHGTFVHANNLQNQ 597
           FHGTF+ A++L  Q
Sbjct: 590 FHGTFIGADDLAKQ 597

[Close]
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 1.8e-238
Identity = 409/586 (69.80%), Postives = 474/586 (80.89%), Query Frame = 0 

Query: 34  KQNSSHNTIIQSALHSPSVLHFPNQTPNFHTNLT-----LKDQT---------------- 93
           K++SS+   I  +L +   LH+P Q     T+ T     +K  T                
Sbjct: 40  KRSSSNTNTITCSLQT---LHYPKQYQPTSTSTTTTPTPIKPTTTTTTTTPHRETKPLSD 99

Query: 94  NKSPF-SNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKH 153
            K PF   WNFLQK A+  LDMVE+ALVSHE +HPLPKTADP+VQI+GNFAPVPE     
Sbjct: 100 TKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQ 159

Query: 154 NLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQ 213
            LPV+G IP CI+GVY+RNGANPL+EP AGHH FDGDGMVHAV   +G ASYACRFT+TQ
Sbjct: 160 ALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQ 219

Query: 214 RLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDR 273
           RL QE+ LGRPVFPKAIGELHGHSGIARLLLFYAR +F LVD   G GVANAGL YFN+ 
Sbjct: 220 RLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNH 279

Query: 274 LLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRK 333
           LLAMSEDDLPYHVRIT +GDL TVGRYDFN QL+STMIAHPK+DPV+ +L+ALSYDVV+K
Sbjct: 280 LLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQK 339

Query: 334 PYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 393
           PYLKYFRFS DG KS DVEIP++ PTMMHDFAITENFVV+PDQQVVFKL EM+ GGSPV+
Sbjct: 340 PYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVV 399

Query: 394 YDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSI 453
           YDKNK SRFGIL KNA D+  + W+++P+ FCFHLWNAWEEPET+E+VVIGSCMTP DSI
Sbjct: 400 YDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSI 459

Query: 454 FNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAI 513
           FNEC+E+LKSVLSEIRLNLRTGKSTRR II  + EQVNLEAGMVNRN+LGRK+++AYLA+
Sbjct: 460 FNECDESLKSVLSEIRLNLRTGKSTRRPII-SDAEQVNLEAGMVNRNKLGRKTQFAYLAL 519

Query: 514 AEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMAFVHD 573
           AEPWPKVSGFAKVDL +GEV+K++YG  KFGGEP FLP        EEE +GYI+AFVHD
Sbjct: 520 AEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP------NGEEEGDGYILAFVHD 579

Query: 574 ERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           E+  KSE+QIVNA +LK+EA+IKLPSRVPYGFHGTF+H+ +L+ QA
Sbjct: 580 EKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

[Close]
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.7e-236
Identity = 401/574 (69.86%), Postives = 476/574 (82.93%), Query Frame = 0 

Query: 24  SFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFL 83
           S S+ N K  ++  S N++      +P++L+FPN  P+ +  +  KD       S WN L
Sbjct: 31  SVSFTNTKPRRRKLSANSVSD----TPNLLNFPNY-PSPNPIIPEKDT------SRWNPL 90

Query: 84  QKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCI 143
           Q+ AS ALD  E+AL+  E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI
Sbjct: 91  QRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCI 150

Query: 144 NGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPV 203
           +GVYLRNGANPLFEP +GHHLFDGDGMVHAV I +G ASY+CRFT+T+RLVQE++LG P+
Sbjct: 151 DGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPI 210

Query: 204 FPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYH 263
           FPKAIGELHGHSGIARL+LFYARG+FGL++HK GTGVANAGL YF+DRLLAMSEDDLPY 
Sbjct: 211 FPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQ 270

Query: 264 VRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDG 323
           VR+T +GDL+T+GR+DF+ QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G
Sbjct: 271 VRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEG 330

Query: 324 TKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGIL 383
            KS DVEIP+ +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGIL
Sbjct: 331 EKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGIL 390

Query: 384 PKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVL 443
           P+NA D+ +++WVESP+TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E L SVL
Sbjct: 391 PRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVL 450

Query: 444 SEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAK 503
           SEIRLNL+TGKSTRR II     Q+NLEAGMVNRN LGRK+RYAYLAIAEPWPKVSGFAK
Sbjct: 451 SEIRLNLKTGKSTRRTII-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAK 510

Query: 504 VDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVN 563
           VDL TGEVK H YGG+K+GGEPFFLP+     E + ED+GYIM+FVHDE + +SE+ IVN
Sbjct: 511 VDLSTGEVKNHFYGGKKYGGEPFFLPR---GLESDGEDDGYIMSFVHDEESWESELHIVN 570

Query: 564 AMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           A+ L++EAT+KLPSRVPYGFHGTFV++ ++ NQA
Sbjct: 571 AVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

[Close]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match: A0A5A7UDK3 (9-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 541/605 (89.42%), Postives = 565/605 (93.39%), Query Frame = 0 

Query: 1   MTA-SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQT 60
           MTA  S+LLDHLGF+NS  KSNF + + N++K I   ++ N+II SALHSPSVL FPNQT
Sbjct: 1   MTAPPSALLDHLGFLNS-TKSNFPNLNINHLKSI---TTQNSIIHSALHSPSVLRFPNQT 60

Query: 61  ------PNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLPKTAD 120
                 PNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLPKTAD
Sbjct: 61  TTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTAD 120

Query: 121 PRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVH 180
           PRVQISGNFAPVPEQPVKHNLPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVH
Sbjct: 121 PRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVH 180

Query: 181 AVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLV 240
           AVTIN G+ASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLV
Sbjct: 181 AVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLV 240

Query: 241 DHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHP 300
           DHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STMIAHP
Sbjct: 241 DHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHP 300

Query: 301 KVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIP 360
           KVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIPVETPTMMHDFAITENFVVIP
Sbjct: 301 KVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIP 360

Query: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420
           DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE
Sbjct: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420

Query: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480
           PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA
Sbjct: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480

Query: 481 GMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEG 540
           GMVN+ R+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ 
Sbjct: 481 GMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDE 540

Query: 541 NSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANN 598
           NS  EEEEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV +NN
Sbjct: 541 NSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNN 599

[Close]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match: A0A1S3AZU0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103484361 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 541/605 (89.42%), Postives = 564/605 (93.22%), Query Frame = 0 

Query: 1   MTA-SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQT 60
           MTA  S LLDHLGF+NS  KSNF + + N++K I   ++ N+II SALHSPSVL FPNQT
Sbjct: 1   MTAPPSPLLDHLGFLNS-TKSNFPNLNINHLKSI---TTQNSIIHSALHSPSVLRFPNQT 60

Query: 61  ------PNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLPKTAD 120
                 PNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLPKTAD
Sbjct: 61  TTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTAD 120

Query: 121 PRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVH 180
           PRVQISGNFAPVPEQPVKHNLPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVH
Sbjct: 121 PRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVH 180

Query: 181 AVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLV 240
           AVTIN G+ASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLV
Sbjct: 181 AVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLV 240

Query: 241 DHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHP 300
           DHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STMIAHP
Sbjct: 241 DHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHP 300

Query: 301 KVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIP 360
           KVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIPVETPTMMHDFAITENFVVIP
Sbjct: 301 KVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIP 360

Query: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420
           DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE
Sbjct: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420

Query: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480
           PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA
Sbjct: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480

Query: 481 GMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEG 540
           GMVN+ R+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ 
Sbjct: 481 GMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDE 540

Query: 541 NSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANN 598
           NS  EEEEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV +NN
Sbjct: 541 NSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNN 599

[Close]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match: A0A0A0KIL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.7e-310
Identity = 542/609 (89.00%), Postives = 565/609 (92.78%), Query Frame = 0 

Query: 1   MTAS-SSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQ- 60
           MTAS S LLDHLGF+NS  KSNF +F+ NN+K I   +S N+II SALHSPSVLHFPNQ 
Sbjct: 1   MTASPSPLLDHLGFLNS-TKSNFPNFNINNLKPI---TSQNSIIHSALHSPSVLHFPNQT 60

Query: 61  ---------TPNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLP 120
                    TPNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLP
Sbjct: 61  TTSTTTTSTTPNFHKSLSLKHQ--KSPTAPNWNLLQKTASMALDMVESALVSHELQHPLP 120

Query: 121 KTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGD 180
           KTADPRVQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGD
Sbjct: 121 KTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGD 180

Query: 181 GMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 240
           GMVHAV+IN G+ASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV
Sbjct: 181 GMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 240

Query: 241 FGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTM 300
           FGLVDHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STM
Sbjct: 241 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTM 300

Query: 301 IAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENF 360
           IAHPKVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIP+ETPTMMHDFAITENF
Sbjct: 301 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENF 360

Query: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420
           VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN
Sbjct: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420

Query: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480
           AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV
Sbjct: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480

Query: 481 NLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540
           NLEAGMVN+NR+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL
Sbjct: 481 NLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540

Query: 541 PKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFV 598
           PKE NS   +EEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV
Sbjct: 541 PKEENS---KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFV 600

[Close]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 4.1e-307
Identity = 522/594 (87.88%), Postives = 549/594 (92.42%), Query Frame = 0 

Query: 4   SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFH 63
           S   LD+LGF NS    N     + NV  I+Q S+HN +I SALHSPSVLHFPN +PNFH
Sbjct: 7   SPPCLDYLGFHNSTKSIN----GFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPNFH 66

Query: 64  TNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAP 123
            NL       KSP SNWNFLQK+AS ALDM ESAL+SHEL+HPLPKTADPRVQISGNFAP
Sbjct: 67  DNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAP 126

Query: 124 VPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASY 183
           VPEQPVKHNLPV GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDG+ASY
Sbjct: 127 VPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASY 186

Query: 184 ACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANA 243
           ACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHKKGTGVANA
Sbjct: 187 ACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANA 246

Query: 244 GLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYA 303
           GL YFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QLDSTMIAHPKVDPVSRELYA
Sbjct: 247 GLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYA 306

Query: 304 LSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEM 363
           LSYDVVRKPYLKYFRFSPDG KSKDVEIP++TPTMMHDFAITENFVVIPDQQVVFKLQEM
Sbjct: 307 LSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEM 366

Query: 364 VKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 423
           +KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPETEEVVVIGS
Sbjct: 367 LKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 426

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRK 483
           CMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMVNRNRLGRK
Sbjct: 427 CMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRK 486

Query: 484 SRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEG 543
           +RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNSG  E+EDEG
Sbjct: 487 TRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG--EKEDEG 546

Query: 544 YIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           YI+AFVHDERT KSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV A +LQNQA
Sbjct: 547 YILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

[Close]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.7e-306
Identity = 520/594 (87.54%), Postives = 549/594 (92.42%), Query Frame = 0 

Query: 4   SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFH 63
           S   LD+LGF NS    N     + NV  I+Q S+HN +I SALHSPSVLHFPN +PNFH
Sbjct: 7   SPPCLDYLGFHNSTKSIN----GFKNVNHIRQPSNHNAVIHSALHSPSVLHFPNHSPNFH 66

Query: 64  TNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAP 123
            NL       KSP SNWNFLQK+AS ALDM ESAL+SHEL+HPLPKTADPRVQISGNFAP
Sbjct: 67  DNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAP 126

Query: 124 VPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASY 183
           VPEQPVK+NLPV GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDG+ASY
Sbjct: 127 VPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASY 186

Query: 184 ACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANA 243
           ACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHKKGTGVANA
Sbjct: 187 ACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANA 246

Query: 244 GLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYA 303
           GL YFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QLDSTMIAHPKVDPVSRELYA
Sbjct: 247 GLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYA 306

Query: 304 LSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEM 363
           LSYDVVRKPYLKYFRFSPDG KSKDVEIP++TPTMMHDFAITENFVVIPDQQVVFKLQEM
Sbjct: 307 LSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEM 366

Query: 364 VKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 423
           +KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPETEEVVVIGS
Sbjct: 367 LKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 426

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRK 483
           CMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMVNRNRLGRK
Sbjct: 427 CMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRK 486

Query: 484 SRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEG 543
           +RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNSG  E+EDEG
Sbjct: 487 TRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG--EKEDEG 546

Query: 544 YIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           YI+AFVHDERT KSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTF+ A +LQNQA
Sbjct: 547 YILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

[Close]
BLAST of Lsi01G001400 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 553/596 (92.79%), Postives = 571/596 (95.81%), Query Frame = 0 

Query: 3   ASSSLLDHLGFVNSIPKSNFHSFSY-NNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPN 62
           +SSSLLDHL F NS  +SNF +F+Y NNVK I QNS+HN II SALHSPSVLHFPNQTPN
Sbjct: 9   SSSSLLDHLSFSNS-TRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTPN 68

Query: 63  FHTNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNF 122
           FH NLTLKDQTNKSPFSNWN LQKTAS AL MVESALVSHELQHPLPKTADPRVQISGNF
Sbjct: 69  FHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNF 128

Query: 123 APVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTA 182
           APVPEQPVKHNLPV GTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVTIN G+A
Sbjct: 129 APVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSA 188

Query: 183 SYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVA 242
           SYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVA
Sbjct: 189 SYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVA 248

Query: 243 NAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSREL 302
           NAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQL STMIAHPKVDP+S+EL
Sbjct: 249 NAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKEL 308

Query: 303 YALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQ 362
           YALSYDVVRKPYLKYFRFSPDG KSKDVEIP+ETPTMMHDFAITENFVVIPDQQVVFKLQ
Sbjct: 309 YALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQ 368

Query: 363 EMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVI 422
           EMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVI
Sbjct: 369 EMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVI 428

Query: 423 GSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLG 482
           GSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ+NLEAGMVNRNRLG
Sbjct: 429 GSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLG 488

Query: 483 RKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEED 542
           RK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNS   +EED
Sbjct: 489 RKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNS---KEED 548

Query: 543 EGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           EGYI+AFVHDERT KSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFV +N+LQNQA
Sbjct: 549 EGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600

[Close]
BLAST of Lsi01G001400 vs. NCBI nr
Match: KAA0052537.1 (9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 541/605 (89.42%), Postives = 565/605 (93.39%), Query Frame = 0 

Query: 1   MTA-SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQT 60
           MTA  S+LLDHLGF+NS  KSNF + + N++K I   ++ N+II SALHSPSVL FPNQT
Sbjct: 1   MTAPPSALLDHLGFLNS-TKSNFPNLNINHLKSI---TTQNSIIHSALHSPSVLRFPNQT 60

Query: 61  ------PNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLPKTAD 120
                 PNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLPKTAD
Sbjct: 61  TTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTAD 120

Query: 121 PRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVH 180
           PRVQISGNFAPVPEQPVKHNLPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVH
Sbjct: 121 PRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVH 180

Query: 181 AVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLV 240
           AVTIN G+ASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLV
Sbjct: 181 AVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLV 240

Query: 241 DHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHP 300
           DHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STMIAHP
Sbjct: 241 DHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHP 300

Query: 301 KVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIP 360
           KVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIPVETPTMMHDFAITENFVVIP
Sbjct: 301 KVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIP 360

Query: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420
           DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE
Sbjct: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420

Query: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480
           PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA
Sbjct: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480

Query: 481 GMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEG 540
           GMVN+ R+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ 
Sbjct: 481 GMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDE 540

Query: 541 NSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANN 598
           NS  EEEEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV +NN
Sbjct: 541 NSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNN 599

[Close]
BLAST of Lsi01G001400 vs. NCBI nr
Match: XP_008439625.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 541/605 (89.42%), Postives = 564/605 (93.22%), Query Frame = 0 

Query: 1   MTA-SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQT 60
           MTA  S LLDHLGF+NS  KSNF + + N++K I   ++ N+II SALHSPSVL FPNQT
Sbjct: 1   MTAPPSPLLDHLGFLNS-TKSNFPNLNINHLKSI---TTQNSIIHSALHSPSVLRFPNQT 60

Query: 61  ------PNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLPKTAD 120
                 PNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLPKTAD
Sbjct: 61  TTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTAD 120

Query: 121 PRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVH 180
           PRVQISGNFAPVPEQPVKHNLPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGDGMVH
Sbjct: 121 PRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVH 180

Query: 181 AVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLV 240
           AVTIN G+ASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLV
Sbjct: 181 AVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLV 240

Query: 241 DHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHP 300
           DHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STMIAHP
Sbjct: 241 DHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHP 300

Query: 301 KVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIP 360
           KVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIPVETPTMMHDFAITENFVVIP
Sbjct: 301 KVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIP 360

Query: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420
           DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE
Sbjct: 361 DQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEE 420

Query: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480
           PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA
Sbjct: 421 PETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEA 480

Query: 481 GMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEG 540
           GMVN+ R+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ 
Sbjct: 481 GMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDE 540

Query: 541 NSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANN 598
           NS  EEEEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV +NN
Sbjct: 541 NSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNN 599

[Close]
BLAST of Lsi01G001400 vs. NCBI nr
Match: XP_004134911.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN49403.1 hypothetical protein Csa_004240 [Cucumis sativus])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.4e-309
Identity = 542/609 (89.00%), Postives = 565/609 (92.78%), Query Frame = 0 

Query: 1   MTAS-SSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQ- 60
           MTAS S LLDHLGF+NS  KSNF +F+ NN+K I   +S N+II SALHSPSVLHFPNQ 
Sbjct: 1   MTASPSPLLDHLGFLNS-TKSNFPNFNINNLKPI---TSQNSIIHSALHSPSVLHFPNQT 60

Query: 61  ---------TPNFHTNLTLKDQTNKSPFS-NWNFLQKTASFALDMVESALVSHELQHPLP 120
                    TPNFH +L+LK Q  KSP + NWN LQKTAS ALDMVESALVSHELQHPLP
Sbjct: 61  TTSTTTTSTTPNFHKSLSLKHQ--KSPTAPNWNLLQKTASMALDMVESALVSHELQHPLP 120

Query: 121 KTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGD 180
           KTADPRVQISGNFAPVPEQ VKH LPV GTIPDCINGVYLRNGANPLF+PTAGHHLFDGD
Sbjct: 121 KTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHLFDGD 180

Query: 181 GMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 240
           GMVHAV+IN G+ASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV
Sbjct: 181 GMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 240

Query: 241 FGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTM 300
           FGLVDHKKGTGVANAGL YFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL STM
Sbjct: 241 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTM 300

Query: 301 IAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENF 360
           IAHPKVDP+S+ELYALSYDVVRKPYLKYFRFSP+G KSKDVEIP+ETPTMMHDFAITENF
Sbjct: 301 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAITENF 360

Query: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420
           VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN
Sbjct: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420

Query: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480
           AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV
Sbjct: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480

Query: 481 NLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540
           NLEAGMVN+NR+GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL
Sbjct: 481 NLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540

Query: 541 PKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFV 598
           PKE NS   +EEDEGYI+AFVHDERT KSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV
Sbjct: 541 PKEENS---KEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFV 600

[Close]
BLAST of Lsi01G001400 vs. NCBI nr
Match: KAG6581433.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034725.1 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.2e-307
Identity = 522/594 (87.88%), Postives = 550/594 (92.59%), Query Frame = 0 

Query: 4   SSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFH 63
           S   LD+LGF NS    N     + NV +I+Q S+HN +I SALHSPSVLHFPN +PNFH
Sbjct: 7   SPPCLDYLGFHNSTKSIN----GFKNVNLIRQPSNHNAVIHSALHSPSVLHFPNHSPNFH 66

Query: 64  TNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAP 123
            NL       KSP SNWNFLQK+AS ALDM ESAL+SHEL+HPLPKTADPRVQISGNFAP
Sbjct: 67  DNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRVQISGNFAP 126

Query: 124 VPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASY 183
           VPEQPVKHNLPV GTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDG+ASY
Sbjct: 127 VPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGSASY 186

Query: 184 ACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANA 243
           ACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHKKGTGVANA
Sbjct: 187 ACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHKKGTGVANA 246

Query: 244 GLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYA 303
           GL YFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QLDSTMIAHPKVDPVSRELYA
Sbjct: 247 GLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVDPVSRELYA 306

Query: 304 LSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEM 363
           LSYDVVRKPYLKYFRFSPDG KSKDVEIP++TPTMMHDFAITENFVVIPDQQVVFKLQEM
Sbjct: 307 LSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQVVFKLQEM 366

Query: 364 VKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 423
           +KGGSPVIYDKNKKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPETEEVVVIGS
Sbjct: 367 LKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPETEEVVVIGS 426

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRK 483
           CMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQVNLEAGMVNRNRLGRK
Sbjct: 427 CMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMVNRNRLGRK 486

Query: 484 SRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEG 543
           +RYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNSG  E+EDEG
Sbjct: 487 TRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG--EKEDEG 546

Query: 544 YIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           YI+AFVHDERT KSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV A +LQNQA
Sbjct: 547 YILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

[Close]
BLAST of Lsi01G001400 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 842.0 bits (2174), Expect = 2.9e-244
Identity = 404/554 (72.92%), Postives = 463/554 (83.57%), Query Frame = 0 

Query: 43  IQSALHSPSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHE 102
           + SALH+P  LHFP Q+ N    + +K +  +S     N  Q+ A+ ALD  E  LVSHE
Sbjct: 50  VSSALHTPPALHFPKQSSN-SPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHE 109

Query: 103 LQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGH 162
             HPLPKTADP VQI+GNFAPV EQPV+ NLPV+G +PD I GVY+RNGANPL EP  GH
Sbjct: 110 KLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGH 169

Query: 163 HLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLL 222
           H FDGDGMVHAV    G+ASYACRFTQT R VQER+LGRPVFPKAIGELHGH+GIARL+L
Sbjct: 170 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 229

Query: 223 FYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNK 282
           FYAR   G+VD   GTGVANAGL YFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ 
Sbjct: 230 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDG 289

Query: 283 QLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDF 342
           QL+STMIAHPKVDP S EL+ALSYDVV KPYLKYFRFSPDGTKS DVEI ++ PTMMHDF
Sbjct: 290 QLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDF 349

Query: 343 AITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTF 402
           AITENFVV+PDQQVVFKL EM++GGSPV+YDKNK +RFGIL K A DS ++ W+++PD F
Sbjct: 350 AITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCF 409

Query: 403 CFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIK 462
           CFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG+STRR II 
Sbjct: 410 CFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 469

Query: 463 EEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFG 522
            E +QVNLEAGMVNRN LGRK+++AYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++G
Sbjct: 470 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYG 529

Query: 523 GEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYG 582
           GEP FLP EG      EEDEGYI+ FVHDE+T KSE+QIVNA+ L+VEAT+KLPSRVPYG
Sbjct: 530 GEPLFLPGEGG-----EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYG 589

Query: 583 FHGTFVHANNLQNQ 597
           FHGTF+ A++L  Q
Sbjct: 590 FHGTFIGADDLAKQ 597

[Close]
BLAST of Lsi01G001400 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 818.9 bits (2114), Expect = 2.6e-237
Identity = 401/574 (69.86%), Postives = 476/574 (82.93%), Query Frame = 0 

Query: 24  SFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPNFHTNLTLKDQTNKSPFSNWNFL 83
           S S+ N K  ++  S N++      +P++L+FPN  P+ +  +  KD       S WN L
Sbjct: 31  SVSFTNTKPRRRKLSANSVSD----TPNLLNFPNY-PSPNPIIPEKDT------SRWNPL 90

Query: 84  QKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIPDCI 143
           Q+ AS ALD  E+AL+  E   PLPKT DPR QISGN+APVPEQ VK +L V G IPDCI
Sbjct: 91  QRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCI 150

Query: 144 NGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLGRPV 203
           +GVYLRNGANPLFEP +GHHLFDGDGMVHAV I +G ASY+CRFT+T+RLVQE++LG P+
Sbjct: 151 DGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPI 210

Query: 204 FPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDLPYH 263
           FPKAIGELHGHSGIARL+LFYARG+FGL++HK GTGVANAGL YF+DRLLAMSEDDLPY 
Sbjct: 211 FPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQ 270

Query: 264 VRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFSPDG 323
           VR+T +GDL+T+GR+DF+ QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYF+FSP+G
Sbjct: 271 VRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEG 330

Query: 324 TKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGIL 383
            KS DVEIP+ +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV YD  K SRFGIL
Sbjct: 331 EKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGIL 390

Query: 384 PKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVL 443
           P+NA D+ +++WVESP+TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E L SVL
Sbjct: 391 PRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVL 450

Query: 444 SEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSGFAK 503
           SEIRLNL+TGKSTRR II     Q+NLEAGMVNRN LGRK+RYAYLAIAEPWPKVSGFAK
Sbjct: 451 SEIRLNLKTGKSTRRTII-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAK 510

Query: 504 VDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMAFVHDERTGKSEVQIVN 563
           VDL TGEVK H YGG+K+GGEPFFLP+     E + ED+GYIM+FVHDE + +SE+ IVN
Sbjct: 511 VDLSTGEVKNHFYGGKKYGGEPFFLPR---GLESDGEDDGYIMSFVHDEESWESELHIVN 570

Query: 564 AMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           A+ L++EAT+KLPSRVPYGFHGTFV++ ++ NQA
Sbjct: 571 AVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

[Close]
BLAST of Lsi01G001400 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 808.1 bits (2086), Expect = 4.6e-234
Identity = 384/517 (74.27%), Postives = 452/517 (87.43%), Query Frame = 0 

Query: 81  NFLQKTASFALDMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVIGTIP 140
           N  QK A+ A+D  E AL+SHE   PLPKTADPRVQI+GN++PVPE  V+ NL V GTIP
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 141 DCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGTASYACRFTQTQRLVQERRLG 200
           DCI+GVY+RNGANP+FEPTAGHHLFDGDGMVHAV I +G+ASYACRFT+T+RLVQE+RLG
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLG 189

Query: 201 RPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDRLLAMSEDDL 260
           RPVFPKAIGELHGHSGIARL+LFYARG+ GL++++ G GVANAGL YFN+RLLAMSEDDL
Sbjct: 190 RPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDL 249

Query: 261 PYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRKPYLKYFRFS 320
           PY ++IT +GDLQTVGRYDF+ QL S MIAHPK+DPV++EL+ALSYDVV+KPYLKYFRFS
Sbjct: 250 PYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFS 309

Query: 321 PDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRF 380
           PDG KS ++EIP+ETPTM+HDFAITENFVVIPDQQVVFKL EM+ G SPV++D  K SR 
Sbjct: 310 PDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRL 369

Query: 381 GILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLK 440
           GI+PK+AT++  +IWV SP+TFCFHLWNAWE PETEE+VVIGSCM+P DSIFNE +E+L+
Sbjct: 370 GIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLR 429

Query: 441 SVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYAYLAIAEPWPKVSG 500
           SVLSEIR+NLRT K+TRR+++    E VNLE GMVNRNRLGRK+R+A+LAIA PWPKVSG
Sbjct: 430 SVLSEIRINLRTRKTTRRSLL--VNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 501 FAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGE-EEEEDEGYIMAFVHDERTGKSEV 560
           FAKVDL TGE+KK+IYGG K+GGEPFFLP  GNSG  EE ED+GYI   VHDE T  SE+
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLP--GNSGNGEENEDDGYIFCHVHDEETKTSEL 549

Query: 561 QIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQ 597
           QI+NA++LK+EATIKLPSRVPYGFHGTFV +N L +Q
Sbjct: 550 QIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

[Close]
BLAST of Lsi01G001400 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 785.0 bits (2026), Expect = 4.2e-227
Identity = 388/597 (64.99%), Postives = 468/597 (78.39%), Query Frame = 0 

Query: 2   TASSSLLDHLGFVNSIPKSNFHSFSYNNVKIIKQNSSHNTIIQSALHSPSVLHFPNQTPN 61
           ++S   L  L F +++         Y +  + K++S  +++       PS     N   N
Sbjct: 74  SSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTN 133

Query: 62  FHTNLTLKDQTNKSPFSNWNFLQKTASFALDMVESALVSHE-LQHPLPKTADPRVQISGN 121
                 +   TN++P    N +Q+TA+  LD VE+A++SHE  +HP PKTADP VQI+GN
Sbjct: 134 L-----IPKTTNQNP--KLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGN 193

Query: 122 FAPVPEQPVKHNLPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTINDGT 181
           F PVPE+PV HNLPV GT+P+CI GVY+RNGANPL +P +GHHLFDGDGMVHAV  ++G+
Sbjct: 194 FFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGS 253

Query: 182 ASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGV 241
            SYACRFT+T RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RG+FGLVD   G GV
Sbjct: 254 VSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGV 313

Query: 242 ANAGLAYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRE 301
           ANAGL YFN  LLAMSEDDLPYHV++T +GDL+T GRYDF+ QL STMIAHPK+DP +RE
Sbjct: 314 ANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRE 373

Query: 302 LYALSYDVVRKPYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKL 361
           L+ALSYDVV KPYLKYFRF+ DG KS DVEIP++ PTM+HDFAITENFVVIPDQQVVF+L
Sbjct: 374 LFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRL 433

Query: 362 QEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVV 421
            EM++GGSPV+YD+ KKSRFGIL KNA D+  + W+E PD FCFHLWN+WEEPET+EVVV
Sbjct: 434 PEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVV 493

Query: 422 IGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRL 481
           IGSCMTPPDSIFNE +E L+SVLSEIRLNL+TG+STRR +I    EQVNLEAGMVNRN L
Sbjct: 494 IGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI---SEQVNLEAGMVNRNLL 553

Query: 482 GRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEE 541
           GRK+RYAYLA+ EPWPKVSGFAKVDL TGE++K+IYG  K+GGEP FLP    SG + EE
Sbjct: 554 GRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP----SG-DGEE 613

Query: 542 DEGYIMAFVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQA 598
           D GYIM FVHDE   KSE+Q++NA+++K+EAT+ LPSRVPYGFHGTF+   +L  QA
Sbjct: 614 DGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQA 655

[Close]
BLAST of Lsi01G001400 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 637.5 bits (1643), Expect = 1.1e-182
Identity = 315/529 (59.55%), Postives = 394/529 (74.48%), Query Frame = 0 

Query: 74  KSPFSNWNFLQKTASFALDMVESALV-SHELQHPLPKTADPRVQISGNFAPVPEQPVKHN 133
           K  + N N LQK A+  LD +ES++V   E   PLPK  DP VQ+SGNFAPV E PV++ 
Sbjct: 57  KPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNG 116

Query: 134 LPVIGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVTIN-DGTASYACRFTQTQ 193
           L V+G IP C+ GVY+RNGANP+F P AGHHLFDGDGM+HAV+I  D   SY+CR+T+T 
Sbjct: 117 LEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTN 176

Query: 194 RLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLAYFNDR 253
           RLVQE  LGR VFPK IGELHGHSG+ARL LF AR   GLVD  +G GVANAG+ +FN R
Sbjct: 177 RLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGR 236

Query: 254 LLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLDSTMIAHPKVDPVSRELYALSYDVVRK 313
           LLAMSEDDLPY V+I   GDL+T+GR+ F+ Q+DS++IAHPKVD  + +L+ LSY+V++K
Sbjct: 237 LLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKK 296

Query: 314 PYLKYFRFSPDGTKSKDVEIPVETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 373
           P+L+Y +F+  G K++DVEI +  PTM+HDFAITENFVVIPDQQ+VFKL EM++GGSPVI
Sbjct: 297 PHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVI 356

Query: 374 YDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEE----VVVIGSCMTP 433
           Y K K +RFG+L K      D+ WV+ PD FCFHLWNAWEE  TEE    +VVIGSCM+P
Sbjct: 357 YVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCMSP 416

Query: 434 PDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAGMVNRNRLGRKSRYA 493
           PD+IF+E  E  +  LSEIRLN+RT +S R+ I+      VNLEAG +NR+ +GRKS++ 
Sbjct: 417 PDTIFSESGEPTRVELSEIRLNMRTKESNRKVIV----TGVNLEAGHINRSYVGRKSQFV 476

Query: 494 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSGEEEEEDEGYIMA 553
           Y+AIA+PWPK SG AKVD+  G V +  YG  +FGGEP F+P EG    E EED+GY+M 
Sbjct: 477 YIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EG----EGEEDKGYVMG 536

Query: 554 FVHDERTGKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVHANNLQNQ 597
           FV DE   +SE  +V+A D+K  A ++LP RVPYGFHGTFV  N L+ Q
Sbjct: 537 FVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:
BLAST of Lsi01G001400 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O240237.6e-25872.769-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
K4CJJ14.2e-25675.589-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9LRR74.1e-24372.929-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9M6E81.8e-23869.809-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9C6Z13.7e-23669.86Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
BLAST of Lsi01G001400 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UDK30.0e+0089.429-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3AZU00.0e+0089.429-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3... [more]
A0A0A0KIL66.7e-31089.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1[more]
A0A6J1EH854.1e-30787.889-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1IVE02.7e-30687.549-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
BLAST of Lsi01G001400 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038883946.10.0e+0092.799-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida][more]
KAA0052537.10.0e+0089.429-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa][more]
XP_008439625.10.0e+0089.42PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ... [more]
XP_004134911.11.4e-30989.009-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN494... [more]
KAG6581433.12.2e-30787.889-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyr... [more]
BLAST of Lsi01G001400 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G14440.12.9e-24472.92nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.12.6e-23769.86nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.14.6e-23474.27nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.14.2e-22764.99nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.11.1e-18259.55nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 122..588
e-value: 7.0E-117
score: 391.4
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 50..597
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 50..597

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G001400.1Lsi01G001400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen