Lsi01G000800 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G000800 vs. ExPASy Swiss-Prot
Match: Q8VYJ1 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE14 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 6.4e-139
Identity = 257/479 (53.65%), Postives = 329/479 (68.68%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           M N+S PHICQCLTR+A+  R + +TV GNR++TG++FVD V +LA GL+ LGL  GDVV
Sbjct: 1   MANHSRPHICQCLTRLASVKRNAVVTVYGNRKRTGREFVDGVLSLAAGLIRLGLRNGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            I+AFNSD +LEWLLAVA VGG+ APLNYRWS +EA  A++ V+PV+LV DE+C  W   
Sbjct: 61  SIAAFNSDLFLEWLLAVALVGGVVAPLNYRWSLKEAKMAMLLVEPVLLVTDETCVSWCID 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGY-NKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFT 180
           +QN  IPSL+W V + S    + N+    LT+E LK+  ++     +  A +   +ICFT
Sbjct: 121 VQNGDIPSLKWRVLMESTSTDFANELNQFLTTEMLKQRTLVPSLATYAWASDDAVVICFT 180

Query: 181 SGTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGAR 240
           SGTTGRPKGV I+H A I Q+LAK+AI GY EDDVYLHT+PL HIGGLSSA+AMLM+GA 
Sbjct: 181 SGTTGRPKGVTISHLAFITQSLAKIAIAGYGEDDVYLHTSPLVHIGGLSSAMAMLMVGAC 240

Query: 241 HILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNG 300
           H+L+PKF+ K+A++ ++   +T  ITVPAMMADL+                R  R   NG
Sbjct: 241 HVLLPKFDAKTALQVMEQNHITCFITVPAMMADLI----------------RVNRTTKNG 300

Query: 301 KTRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSI 360
                V+KILNGGG L  EL+K+A+  FP A+++SAYGMTE CSSLTF+ L DP  E+  
Sbjct: 301 AENRGVRKILNGGGSLSSELLKEAVNIFPCARILSAYGMTEACSSLTFMTLHDPTQESFK 360

Query: 361 PHFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLD-GSSHVGRILTRGPHLMLRYYDKI 420
             +      K       +G CVGKPAPHIE+ + LD  SS VG+ILTRGPH MLRY+   
Sbjct: 361 VTYPLLNQPK-------QGTCVGKPAPHIELMVKLDEDSSRVGKILTRGPHTMLRYWGHQ 420

Query: 421 SARPSNDV-----NEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLL 473
            A+ + +      NE W DTGDIG+ D+ GNLWL+GRSNGRIK+GGEN+YPEEV   L+
Sbjct: 421 VAQENVETSESRSNEAWLDTGDIGAFDEFGNLWLIGRSNGRIKTGGENVYPEEVEAVLV 456

BLAST of Lsi01G000800 vs. ExPASy Swiss-Prot
Match: P23971 (2-succinylbenzoate--CoA ligase OS=Bacillus subtilis (strain 168) OX=224308 GN=menE PE=1 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 1.2e-31
Identity = 102/326 (31.29%), Postives = 151/326 (46.32%), Query Frame = 0

Query: 152 EELKKHYVINIPPNFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKVAIVGYTE 211
           +EL K     I        +  A + +TSGTTG+PKGV  T        ++    +G TE
Sbjct: 125 DELMKEAAEEIEIEAYMQMDATATLMYTSGTTGKPKGVQQTFGNHYFSAVSSALNLGITE 184

Query: 212 DDVYLHTAPLCHIGGLSSAIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMA 271
            D +L   PL HI GLS+    ++ G   +L  +F V   + +I+ + VT +  V  M+A
Sbjct: 185 QDRWLIALPLFHISGLSALFKSVIYGMTVVLHQRFSVSDVLHSINRHEVTMISAVQTMLA 244

Query: 272 DLVSLIRCLYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAM-KFFPRA 331
            L+                      +  +  E+++ IL GGG  PL L+++   K FP  
Sbjct: 245 SLL---------------------EETNRCPESIRCILLGGGPAPLPLLEECREKGFP-- 304

Query: 332 KLVSAYGMTETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHIEI 391
            +  +YGMTETCS +           T  P F   +               GKP    EI
Sbjct: 305 -VFQSYGMTETCSQIV----------TLSPEFSMEKLGS-----------AGKPLFSCEI 364

Query: 392 KICLDGS----SHVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLW 451
           KI  DG        G I+ +GP++M  Y+++ SA  ++  N  W  TGD+G +D+ G L+
Sbjct: 365 KIERDGQVCEPYEHGEIMVKGPNVMKSYFNRESANEASFQNG-WLKTGDLGYLDNEGFLY 404

Query: 452 LVGRSNGRIKSGGENIYPEEVSLFLL 473
           ++ R +  I SGGENIYP EV   LL
Sbjct: 425 VLDRRSDLIISGGENIYPAEVESVLL 404

BLAST of Lsi01G000800 vs. ExPASy Swiss-Prot
Match: A7Z809 (2-succinylbenzoate--CoA ligase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) OX=326423 GN=menE PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 5.8e-31
Identity = 106/333 (31.83%), Postives = 155/333 (46.55%), Query Frame = 0

Query: 152 EELKKHYVINIPPNFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKVAIVGYTE 211
           +EL+     +I P      +  A + +TSGTTGRPKGV  T        ++    +G TE
Sbjct: 126 DELQAEEAGDIEPEAYVTLDDTATLMYTSGTTGRPKGVQQTFGNHYSSAVSSALNLGVTE 185

Query: 212 DDVYLHTAPLCHIGGLSSAIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMA 271
            D +L   PL HI GLS+    ++ G   +L  KF V   +++I  + VT +  V  M++
Sbjct: 186 RDRWLIALPLFHISGLSALFKSVIYGMPVVLHQKFSVTDVLDSISSHQVTIISAVQTMLS 245

Query: 272 DLVSLIRCLYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDA-MKFFPRA 331
            L++                     +  +  E+++ IL GGG  PL L+++   K FP  
Sbjct: 246 GLLA---------------------ETEQCPESLRCILLGGGPAPLPLLEECRRKQFP-- 305

Query: 332 KLVSAYGMTETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHIEI 391
            +  +YG+TETCS +           T  P F      K  S         GKP    EI
Sbjct: 306 -VFQSYGLTETCSQIV----------TLSPEFSM---DKLGS--------AGKPLFSCEI 365

Query: 392 KICLDGSS----HVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLW 451
           +I  DG+       G I  +GP++M  YY +  A  +   N  WF TGD+G +DD G L+
Sbjct: 366 RIEKDGNPCAPFEHGEITVKGPNVMKGYYHRDDANQAAFHNG-WFKTGDLGYLDDEGFLY 412

Query: 452 LVGRSNGRIKSGGENIYPEEVSLFLLIWIVIRE 480
           ++ R +  I SGGENIYP EV   LL    + E
Sbjct: 426 VLDRRSDLIISGGENIYPAEVEAALLAHPAVAE 412

BLAST of Lsi01G000800 vs. ExPASy Swiss-Prot
Match: A0RK73 (2-succinylbenzoate--CoA ligase OS=Bacillus thuringiensis (strain Al Hakam) OX=412694 GN=menE PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.7e-30
Identity = 118/452 (26.11%), Postives = 194/452 (42.92%), Query Frame = 0

Query: 37  QFVDSVFALARGLLDLGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEA 96
           Q  + V ++   L  +G+  G  V +   N    +  + A+++VG +A  LN R S EE 
Sbjct: 33  QLHEKVVSVCEHLTHVGVKRGQKVAVLMKNGMEMITVIHALSYVGAVAVLLNTRLSREEL 92

Query: 97  ISAIVAVQPVMLVIDESCCLWYSKLQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKK 156
           +  +   + + LV D+                     F    +P Y+             
Sbjct: 93  LWQMDDAEVICLVTDQD--------------------FEAKDIPVYSFAE---------- 152

Query: 157 HYVINIPPNFLHAPEGVAL-----ICFTSGTTGRPKGVAITHSALIVQTLAKVAIVGYTE 216
             V+N P       E  +L     I +TSGTTG+PKGV +T+       +     +G  +
Sbjct: 153 --VMNGPKEEASIQEEFSLREAMTIIYTSGTTGKPKGVILTYGNHWASAVGSSLNLGLRD 212

Query: 217 DDVYLHTAPLCHIGGLSSAIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMA 276
           DD +L   P+ H+GGLS  +  +M G R +L+PK++     +A+   GVT +  V  M+ 
Sbjct: 213 DDCWLACMPMFHVGGLSLLMKNIMYGMRILLVPKYDADFIHKALQTRGVTIISVVSKMLT 272

Query: 277 DLVSLIRCLYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAM-KFFPRA 336
           DL+  +                     G    +++ +L GGG  P  L++  + K  P  
Sbjct: 273 DLLERL-------------------GEGTYPSSLRCMLLGGGPAPKPLLETCVDKGIP-- 332

Query: 337 KLVSAYGMTETCSSLTFL---MLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPH 396
            +   YGMTET S +  L    +   +G    P FQ      C    + +GV V    P 
Sbjct: 333 -VYQTYGMTETSSQICTLSADYMLTKVGSAGKPLFQ------CQLRIEKDGVVV---PPF 392

Query: 397 IEIKICLDGSSHVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLWL 456
           +E           G I+ +GP++   Y+++  A     +   W  TGD+G +D+ G L++
Sbjct: 393 VE-----------GEIVVKGPNVTGGYFNREDA-TRETIQNGWLHTGDLGYLDEEGFLYV 409

Query: 457 VGRSNGRIKSGGENIYPEEVSLFLLIWIVIRE 480
           + R +  I SGGENIYP ++   LL   ++ E
Sbjct: 453 LDRRSDLIISGGENIYPAQIEEVLLSHPMVAE 409

BLAST of Lsi01G000800 vs. ExPASy Swiss-Prot
Match: Q72YK9 (2-succinylbenzoate--CoA ligase OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=menE PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.9e-30
Identity = 116/446 (26.01%), Postives = 192/446 (43.05%), Query Frame = 0

Query: 34  TGQQFVDSVFALARGLLDLGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSF 93
           T  Q  + V ++   L  +G+  G  V +   N    +  + A+++VG +A  LN R S 
Sbjct: 29  TFMQLHEKVVSVCEHLTHIGVKRGQKVAVLMKNGMEMITVIHALSYVGAVAVLLNTRLSR 88

Query: 94  EEAISAIVAVQPVMLVIDESCCLWYSKLQNDHIPSLRWHVFIGSHLPGYN---KTRGALT 153
           EE +  +   + + LV D+                     F    +P Y+      G   
Sbjct: 89  EELLWQMDDAEVICLVTDQD--------------------FDAKDIPVYSFAEVMNGPKE 148

Query: 154 SEELKKHYVINIPPNFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKVAIVGYT 213
              +++ + +          E    I +TSGTTG+PKGV +T+       +     +G  
Sbjct: 149 EASIQEEFSL----------EEAMTIIYTSGTTGKPKGVILTYGNHWASAVGSSLNLGLR 208

Query: 214 EDDVYLHTAPLCHIGGLSSAIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMM 273
           +DD +L   P+ H+GGLS  +  +M G R +L+PK++     +A+   GVT +  V  M+
Sbjct: 209 DDDCWLACMPMFHVGGLSLLMKNIMYGMRILLVPKYDADFIHKALQTRGVTIISVVSKML 268

Query: 274 ADLVSLIRCLYLCFVGLIFERFFRERDNGKT-RETVKKILNGGGGLPLELIKDAMKFFPR 333
            DL+                    ER   +T   +++ +L GGG  P  L++  ++    
Sbjct: 269 TDLL--------------------ERLGAETYPSSLRCMLLGGGPAPKPLLETCVE--KG 328

Query: 334 AKLVSAYGMTETCSSLTFL---MLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAP 393
             +   YGMTET S +  L    +   +G    P FQ      C    + +GV V  PA 
Sbjct: 329 IPVYQTYGMTETSSQICTLSADYMLTKVGSAGKPLFQ------CQLRIEKDGVVV--PA- 388

Query: 394 HIEIKICLDGSSHVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLW 453
                         G I+ +GP++   Y+++  A     +   W  TGDIG +D+ G L+
Sbjct: 389 -----------FTEGEIVVKGPNVTGGYFNREDA-TRETIQNGWLHTGDIGYLDEEGFLY 401

Query: 454 LVGRSNGRIKSGGENIYPEEVSLFLL 473
           ++ R +  I SGGENIYP ++   LL
Sbjct: 449 VLDRRSDLIISGGENIYPAQIEEVLL 401

BLAST of Lsi01G000800 vs. ExPASy TrEMBL
Match: A0A5A7UBB8 (2-succinylbenzoate--CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00870 PE=4 SV=1)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 617/703 (87.77%), Postives = 634/703 (90.18%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAG+RRQTGQQFVDSVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGDRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            ISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEE +SA+VAV+PVMLVIDE  C WYSK
Sbjct: 61  AISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEETLSAMVAVRPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IG H PGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGFHPPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVK AVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKPAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPM ETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVNIFPRAKLVSAYGMTETCSSLTFLMLCDPMRETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTNIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRRISKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRISKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 657

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFGELAT 704
           TLKSRD +NASTMKTIYNARHRQKRVEKKRKPDMQ+LFGELAT
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKRKPDMQELFGELAT 657

BLAST of Lsi01G000800 vs. ExPASy TrEMBL
Match: A0A0A0KIV8 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525490 PE=4 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 616/703 (87.62%), Postives = 637/703 (90.61%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA R SPLT+A NR QTGQQFV+SVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATAPRPSPLTLAANRPQTGQQFVNSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEEA+SA+VAVQPVMLVIDE  C WYSK
Sbjct: 61  VISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEEALSAMVAVQPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQN+HIPSLRWHVFIGSHLPGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNNHIPSLRWHVFIGSHLPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPMGETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVGIFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           G+SSTSYT IDSSC VDYT+EFTTNEIFK REALINWTREVGK+
Sbjct: 481 ----------------GNSSTSYTNIDSSCEVDYTNEFTTNEIFKGREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRR+SKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRMSKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 657

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFGELAT 704
           TLKSRD +NASTMKTIYNARHRQKRVEKK+KPDMQ+LFGELAT
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKQKPDMQELFGELAT 657

BLAST of Lsi01G000800 vs. ExPASy TrEMBL
Match: A0A1S3AZM9 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484311 PE=4 SV=1)

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 614/699 (87.84%), Postives = 631/699 (90.27%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAG+RRQTGQQFVDSVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGDRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            ISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEE +SA+VAV+PVMLVIDE  C WYSK
Sbjct: 61  AISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEETLSAMVAVRPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IG H PGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGFHPPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVK AVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKPAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPMGETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVSIFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTSIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRRISKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRISKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 653

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD +NASTMKTIYNARHRQKRVEKKRKPDMQ+LFG
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKRKPDMQELFG 653

BLAST of Lsi01G000800 vs. ExPASy TrEMBL
Match: A0A6J1ED26 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433227 PE=4 SV=1)

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 592/699 (84.69%), Postives = 620/699 (88.70%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYS  HICQCL+RIATA RAS LT+AGNRRQTG+QFV+SV ALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSHSHICQCLSRIATAHRASSLTIAGNRRQTGEQFVNSVLALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISA NSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISA+VAVQPVMLVIDESCC WYSK
Sbjct: 61  VISASNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAMVAVQPVMLVIDESCCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           L+N  IPSLRW V IGSH PGY+KTRGALT+EELKK++V+++P +FLHAPE  ALICFTS
Sbjct: 121 LRNTDIPSLRWQVLIGSHRPGYDKTRGALTTEELKKYHVVSLPLDFLHAPEDAALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGV ITHSALIVQTLAKVA VGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVVITHSALIVQTLAKVANVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
            RE+VKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDP GETSIP
Sbjct: 301 RRESVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPTGETSIP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSY  S CHS+YQPEGVCVGKPAPH+E+KICLD SSHVGRILTRGPHLMLRYYDKIS 
Sbjct: 361 HFQSYAQSNCHSIYQPEGVCVGKPAPHVEMKICLDDSSHVGRILTRGPHLMLRYYDKISP 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GH STSYT IDSSC VDY++EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHLSTSYTNIDSSCEVDYSNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
            GLVIVIKTSDAGVNGRRPRISFGCERSGSYRR+SKLE L D+ ARKLTGTKKCGCPFLL
Sbjct: 541 NGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRLSKLEVLEDRGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTD DWMVKVVCG+HNH+ANKNMEGH+FAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDGDWMVKVVCGVHNHVANKNMEGHSFAGRLSQEENEILMNLSNSSVRPKDILS 653

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD RNASTMKTIYNARHRQKRVEKK+KPDMQQLFG
Sbjct: 661 TLKSRDARNASTMKTIYNARHRQKRVEKKQKPDMQQLFG 653

BLAST of Lsi01G000800 vs. ExPASy TrEMBL
Match: A0A6J1IL95 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478058 PE=4 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 593/699 (84.84%), Postives = 621/699 (88.84%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYS  HICQCL+RIATA RASPLT+ GNRRQTG+QFV+SV ALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSHSHICQCLSRIATAHRASPLTITGNRRQTGEQFVNSVLALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISA NSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISA+VAVQPVMLVIDESCC WYSK
Sbjct: 61  VISASNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAMVAVQPVMLVIDESCCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           L+N+ IPSLRW V IGSHL GY+KTRGALTSEELKK++V+++P +FLHAPE  ALICFTS
Sbjct: 121 LRNNDIPSLRWQVLIGSHLTGYDKTRGALTSEELKKYHVVSLPLDFLHAPEDAALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVA VGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVANVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
            RE+VKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDP GETSIP
Sbjct: 301 RRESVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPTGETSIP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSY  SKCHS+YQPEGVCVGKPAPH+E+KICLD SSHVGRILTRG +LMLRYYDKIS 
Sbjct: 361 HFQSYAQSKCHSIYQPEGVCVGKPAPHVEMKICLDDSSHVGRILTRGLNLMLRYYDKISP 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDY++EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTNIDSSCEVDYSNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
            G VIVIKTSDAGVNGRRPRISFGCERSGSYRR+SKLE L DK ARKLTGTKKCGCPFLL
Sbjct: 541 NGFVIVIKTSDAGVNGRRPRISFGCERSGSYRRLSKLEVLEDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTD DWMVKVVCG+HNH+ANKNMEGH+FAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDGDWMVKVVCGVHNHVANKNMEGHSFAGRLSQEENEILMNLSNSSVRPKDILS 653

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD RNASTMKTIYNARHRQKRVE K+KPDMQQLFG
Sbjct: 661 TLKSRDARNASTMKTIYNARHRQKRVENKQKPDMQQLFG 653

BLAST of Lsi01G000800 vs. NCBI nr
Match: XP_038882137.1 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 630/699 (90.13%), Postives = 641/699 (91.70%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPG VV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGHVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISAFNSDWYLEWLLAV FVGGIAAPLNYRWSFEEAISAIV+VQPVMLV DESCC WYSK
Sbjct: 61  VISAFNSDWYLEWLLAVTFVGGIAAPLNYRWSFEEAISAIVSVQPVMLVTDESCCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IGSHLPGY KTRGAL+SEELKKHYVINIPPNFLHA EGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGSHLPGYEKTRGALSSEELKKHYVINIPPNFLHAAEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALI+Q LAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIMQALAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI             RFFRERD+GK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI-------------RFFRERDSGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+K FPRAKLVSAYGMTETCSSLTFLML DPMGETSIP
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVKIFPRAKLVSAYGMTETCSSLTFLMLSDPMGETSIP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSNDVNEVWFDTGDIGSIDD+GNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDVNEVWFDTGDIGSIDDSGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTNIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLE LG+KRARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEVLGEKRARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 656

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD RNASTMKTIYNARHRQKRVEKKRKPDMQQLFG
Sbjct: 661 TLKSRDARNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 656

BLAST of Lsi01G000800 vs. NCBI nr
Match: XP_038882138.1 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X2 [Benincasa hispida])

HSP 1 Score: 1244.6 bits (3219), Expect = 0.0e+00
Identity = 627/699 (89.70%), Postives = 638/699 (91.27%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPG VV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGHVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISAFNSDWYLEWLLAV FVGGIAAPLNYRWSFEEAISAIV+VQPVMLV DESCC WYSK
Sbjct: 61  VISAFNSDWYLEWLLAVTFVGGIAAPLNYRWSFEEAISAIVSVQPVMLVTDESCCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IGSHLPGY KTRGAL+SEELKKHYVINIPPNFLHA EGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGSHLPGYEKTRGALSSEELKKHYVINIPPNFLHAAEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALI+Q LAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIMQALAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI                RERD+GK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDSGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+K FPRAKLVSAYGMTETCSSLTFLML DPMGETSIP
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVKIFPRAKLVSAYGMTETCSSLTFLMLSDPMGETSIP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSNDVNEVWFDTGDIGSIDD+GNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDVNEVWFDTGDIGSIDDSGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTNIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLE LG+KRARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEVLGEKRARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 653

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD RNASTMKTIYNARHRQKRVEKKRKPDMQQLFG
Sbjct: 661 TLKSRDARNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 653

BLAST of Lsi01G000800 vs. NCBI nr
Match: KAA0052488.1 (2-succinylbenzoate--CoA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 617/703 (87.77%), Postives = 634/703 (90.18%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAG+RRQTGQQFVDSVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGDRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            ISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEE +SA+VAV+PVMLVIDE  C WYSK
Sbjct: 61  AISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEETLSAMVAVRPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IG H PGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGFHPPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVK AVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKPAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPM ETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVNIFPRAKLVSAYGMTETCSSLTFLMLCDPMRETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTNIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRRISKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRISKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 657

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFGELAT 704
           TLKSRD +NASTMKTIYNARHRQKRVEKKRKPDMQ+LFGELAT
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKRKPDMQELFGELAT 657

BLAST of Lsi01G000800 vs. NCBI nr
Match: XP_031742983.1 (2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X2 [Cucumis sativus] >XP_031742984.1 2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X2 [Cucumis sativus] >KGN49463.1 hypothetical protein Csa_002735 [Cucumis sativus])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 616/703 (87.62%), Postives = 637/703 (90.61%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA R SPLT+A NR QTGQQFV+SVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATAPRPSPLTLAANRPQTGQQFVNSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
           VISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEEA+SA+VAVQPVMLVIDE  C WYSK
Sbjct: 61  VISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEEALSAMVAVQPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQN+HIPSLRWHVFIGSHLPGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNNHIPSLRWHVFIGSHLPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPMGETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVGIFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           G+SSTSYT IDSSC VDYT+EFTTNEIFK REALINWTREVGK+
Sbjct: 481 ----------------GNSSTSYTNIDSSCEVDYTNEFTTNEIFKGREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRR+SKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRMSKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 657

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFGELAT 704
           TLKSRD +NASTMKTIYNARHRQKRVEKK+KPDMQ+LFGELAT
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKQKPDMQELFGELAT 657

BLAST of Lsi01G000800 vs. NCBI nr
Match: XP_008439545.1 (PREDICTED: 2-succinylbenzoate--CoA ligase, chloroplastic/peroxisomal isoform X1 [Cucumis melo])

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 614/699 (87.84%), Postives = 631/699 (90.27%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           MTNYSLPHICQCLTRIATA RASPLTVAG+RRQTGQQFVDSVFALARGLLDLGLLPGDVV
Sbjct: 1   MTNYSLPHICQCLTRIATARRASPLTVAGDRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            ISAFNSDWYLEWLLAVAFVG IAAPLNYRWSFEE +SA+VAV+PVMLVIDE  C WYSK
Sbjct: 61  AISAFNSDWYLEWLLAVAFVGAIAAPLNYRWSFEETLSAMVAVRPVMLVIDEGGCQWYSK 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFTS 180
           LQNDHIPSLRWHV IG H PGY+KTRGALTSEELKKHYVIN+PPNFLHAPEGVALICFTS
Sbjct: 121 LQNDHIPSLRWHVLIGFHPPGYDKTRGALTSEELKKHYVINMPPNFLHAPEGVALICFTS 180

Query: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240
           GTTGRPKGVAITHSALIVQTLAKVAIVGY+EDDVYLHTAPLCHIGGLSSAIAMLMLGARH
Sbjct: 181 GTTGRPKGVAITHSALIVQTLAKVAIVGYSEDDVYLHTAPLCHIGGLSSAIAMLMLGARH 240

Query: 241 ILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNGK 300
           ILIPKFEVK AVEAIDLYGVTSLITVPAMMADLVSLI                RERDNGK
Sbjct: 241 ILIPKFEVKPAVEAIDLYGVTSLITVPAMMADLVSLI----------------RERDNGK 300

Query: 301 TRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSIP 360
           TRE+VKKILNGGGGLPLELIKDA+  FPRAKLVSAYGMTETCSSLTFLMLCDPMGETS+P
Sbjct: 301 TRESVKKILNGGGGLPLELIKDAVSIFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSVP 360

Query: 361 HFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSHVGRILTRGPHLMLRYYDKISA 420
           HFQSYEHSKC SVYQPEGVCVGKPAPHIEIKI LDGSS+VGRILTRGPHLMLRYYDKISA
Sbjct: 361 HFQSYEHSKCRSVYQPEGVCVGKPAPHIEIKISLDGSSNVGRILTRGPHLMLRYYDKISA 420

Query: 421 RPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLLIWIVIREL 480
           RPSND N+VWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE              
Sbjct: 421 RPSNDANDVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEE-------------- 480

Query: 481 LDSSHIMLVTGQANTGGHSSTSYTKIDSSCAVDYTDEFTTNEIFKSREALINWTREVGKK 540
                           GHSSTSYT IDSSC VDYT+EFTTNEIFKSREALINWTREVGK+
Sbjct: 481 ----------------GHSSTSYTSIDSSCEVDYTNEFTTNEIFKSREALINWTREVGKR 540

Query: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGSYRRISKLEALGDKRARKLTGTKKCGCPFLL 600
           KGLVIVIKTSDAGVNGRRPRISFGCERSG YRRISKLE LGDK ARKLTGTKKCGCPFLL
Sbjct: 541 KGLVIVIKTSDAGVNGRRPRISFGCERSGRYRRISKLEVLGDKGARKLTGTKKCGCPFLL 600

Query: 601 KGQKLTTDDDWMVKVVCGIHNHLANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 660
           KGQKLTTDDDWMVKVVCGIHNH+ANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS
Sbjct: 601 KGQKLTTDDDWMVKVVCGIHNHVANKNMEGHTFAGRLSQEENEILMNLSNSSVRPKDILS 653

Query: 661 TLKSRDTRNASTMKTIYNARHRQKRVEKKRKPDMQQLFG 700
           TLKSRD +NASTMKTIYNARHRQKRVEKKRKPDMQ+LFG
Sbjct: 661 TLKSRDAQNASTMKTIYNARHRQKRVEKKRKPDMQELFG 653

BLAST of Lsi01G000800 vs. TAIR 10
Match: AT1G30520.1 (acyl-activating enzyme 14 )

HSP 1 Score: 496.1 bits (1276), Expect = 4.6e-140
Identity = 257/479 (53.65%), Postives = 329/479 (68.68%), Query Frame = 0

Query: 1   MTNYSLPHICQCLTRIATACRASPLTVAGNRRQTGQQFVDSVFALARGLLDLGLLPGDVV 60
           M N+S PHICQCLTR+A+  R + +TV GNR++TG++FVD V +LA GL+ LGL  GDVV
Sbjct: 1   MANHSRPHICQCLTRLASVKRNAVVTVYGNRKRTGREFVDGVLSLAAGLIRLGLRNGDVV 60

Query: 61  VISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQPVMLVIDESCCLWYSK 120
            I+AFNSD +LEWLLAVA VGG+ APLNYRWS +EA  A++ V+PV+LV DE+C  W   
Sbjct: 61  SIAAFNSDLFLEWLLAVALVGGVVAPLNYRWSLKEAKMAMLLVEPVLLVTDETCVSWCID 120

Query: 121 LQNDHIPSLRWHVFIGSHLPGY-NKTRGALTSEELKKHYVINIPPNFLHAPEGVALICFT 180
           +QN  IPSL+W V + S    + N+    LT+E LK+  ++     +  A +   +ICFT
Sbjct: 121 VQNGDIPSLKWRVLMESTSTDFANELNQFLTTEMLKQRTLVPSLATYAWASDDAVVICFT 180

Query: 181 SGTTGRPKGVAITHSALIVQTLAKVAIVGYTEDDVYLHTAPLCHIGGLSSAIAMLMLGAR 240
           SGTTGRPKGV I+H A I Q+LAK+AI GY EDDVYLHT+PL HIGGLSSA+AMLM+GA 
Sbjct: 181 SGTTGRPKGVTISHLAFITQSLAKIAIAGYGEDDVYLHTSPLVHIGGLSSAMAMLMVGAC 240

Query: 241 HILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRCLYLCFVGLIFERFFRERDNG 300
           H+L+PKF+ K+A++ ++   +T  ITVPAMMADL+                R  R   NG
Sbjct: 241 HVLLPKFDAKTALQVMEQNHITCFITVPAMMADLI----------------RVNRTTKNG 300

Query: 301 KTRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMTETCSSLTFLMLCDPMGETSI 360
                V+KILNGGG L  EL+K+A+  FP A+++SAYGMTE CSSLTF+ L DP  E+  
Sbjct: 301 AENRGVRKILNGGGSLSSELLKEAVNIFPCARILSAYGMTEACSSLTFMTLHDPTQESFK 360

Query: 361 PHFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLD-GSSHVGRILTRGPHLMLRYYDKI 420
             +      K       +G CVGKPAPHIE+ + LD  SS VG+ILTRGPH MLRY+   
Sbjct: 361 VTYPLLNQPK-------QGTCVGKPAPHIELMVKLDEDSSRVGKILTRGPHTMLRYWGHQ 420

Query: 421 SARPSNDV-----NEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGGENIYPEEVSLFLL 473
            A+ + +      NE W DTGDIG+ D+ GNLWL+GRSNGRIK+GGEN+YPEEV   L+
Sbjct: 421 VAQENVETSESRSNEAWLDTGDIGAFDEFGNLWLIGRSNGRIKTGGENVYPEEVEAVLV 456

BLAST of Lsi01G000800 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 118.2 bits (295), Expect = 2.6e-26
Identity = 117/433 (27.02%), Postives = 176/433 (40.65%), Query Frame = 0

Query: 45  LARGLLDLGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQ 104
           LA GL +LG+   DVV+I   NS   +   LA +F+G I    N  ++  E      A  
Sbjct: 76  LAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASA 135

Query: 105 PVMLVIDESCCLWYSKLQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPP 164
             ++V           LQND +  +         +P        LT  E  +  V +IP 
Sbjct: 136 AKLIVTQSRYVDKIKNLQNDGVLIVTTD---SDAIPENCLRFSELTQSEEPR--VDSIPE 195

Query: 165 NFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKV----AIVGYTEDDVYLHTAP 224
               +PE V  + F+SGTTG PKGV +TH  L+     +V      + +  DDV L   P
Sbjct: 196 KI--SPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLP 255

Query: 225 LCHIGGLSS-AIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRC 284
           + HI  L+S  +  L +GA  +++PKFE+   +E I    VT  + VP ++  +      
Sbjct: 256 MFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAK---- 315

Query: 285 LYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMT 344
                                   +V+ + +G   L  EL       FP AKL   YGMT
Sbjct: 316 --------------SPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMT 375

Query: 345 ETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSH 404
           E        +L   +G    P     +   C +V +   + +  P     +       + 
Sbjct: 376 EAGP-----VLAMSLGFAKEPF--PVKSGACGTVVRNAEMKILDPDTGDSLP-----RNK 435

Query: 405 VGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGG 464
            G I  RG  +M  Y +   A  S    + W  TGD+G IDD+  L++V R    IK  G
Sbjct: 436 PGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKG 471

Query: 465 ENIYPEEVSLFLL 473
             + P E+   L+
Sbjct: 496 FQVAPAELESLLI 471

BLAST of Lsi01G000800 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 113.2 bits (282), Expect = 8.3e-25
Identity = 119/451 (26.39%), Postives = 195/451 (43.24%), Query Frame = 0

Query: 42  VFALARGLLD-LGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAI 101
           V ++A G+   LG+  GDVV +   NS ++    L++  +G I   +N   S  E     
Sbjct: 82  VQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQ- 141

Query: 102 VAVQPVMLVIDESCCLWYSKLQN-DHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYV 161
                   V + S  L ++  +N + + SL   V   S    ++  R      E  K Y 
Sbjct: 142 --------VSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-----IENPKFYS 201

Query: 162 I-----NIPPNFLHAPEGVALICFTSGTTGRPKGVAITHSALI--VQTLAKVAIVGYT-- 221
           I        P  L   + VA I ++SGTTG  KGV +TH  LI  ++   +     Y   
Sbjct: 202 IMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYP 261

Query: 222 -EDDVYLHTAPLCHIGGLS-SAIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPA 281
              +VYL   PLCHI GLS   + +L LG+  +++ +F+    V  I+ + +T    VP 
Sbjct: 262 GSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPP 321

Query: 282 MMADLVSLIRCLYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAMKFFP 341
           M+  L    +   +C               G+  +++K++ +G   L  + I+D ++  P
Sbjct: 322 MLMALTK--KAKGVC---------------GEVFKSLKQVSSGAAPLSRKFIEDFLQTLP 381

Query: 342 RAKLVSAYGMTETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHI 401
              L+  YGMTE+ +                  F S + S+  S        VG  AP++
Sbjct: 382 HVDLIQGYGMTESTA-------------VGTRGFNSEKLSRYSS--------VGLLAPNM 441

Query: 402 EIKICLDGSS-------HVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDD 461
           + K+ +D SS       + G +  +GP +M  Y +   A   + V + W  TGDI   D+
Sbjct: 442 QAKV-VDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDE 479

Query: 462 NGNLWLVGRSNGRIKSGGENIYPEEVSLFLL 473
           +G L++V R    IK  G  I P ++   L+
Sbjct: 502 DGYLFIVDRIKEIIKYKGFQIAPADLEAVLV 479

BLAST of Lsi01G000800 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 110.2 bits (274), Expect = 7.1e-24
Identity = 110/434 (25.35%), Postives = 186/434 (42.86%), Query Frame = 0

Query: 45  LARGLLDLGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQ 104
           +A G+  LG+  GDVV++   NS  +    LAVA++G ++   N  ++  E      A  
Sbjct: 86  IAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASA 145

Query: 105 PVMLVIDESCCLWYSKLQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPP 164
             M++  +      + L+ND +  +      G +    +   G ++  EL +     +  
Sbjct: 146 AKMIITKKCLVDKLTNLKNDGVLIVCLD-DDGDNGVVSSSDDGCVSFTELTQADETELLK 205

Query: 165 NFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKV----AIVGYTEDDVYLHTAP 224
             + +PE    + ++SGTTG PKGV ITH  L+     KV      + +T +DV L   P
Sbjct: 206 PKI-SPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLP 265

Query: 225 LCHIGGLSS-AIAMLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRC 284
           + HI  L +  ++ +  GA  +++P+FE+   +E I  Y VT +   P ++   +     
Sbjct: 266 MFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPET 325

Query: 285 LYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAMKF-FPRAKLVSAYGM 344
                     ER+           +V+ +L+G   L  EL +DA++  FP A     YGM
Sbjct: 326 ----------ERY--------DLSSVRIMLSGAATLKKEL-EDAVRLKFPNAIFGQGYGM 385

Query: 345 TETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSS 404
           TE+ +    L       +T        +   C +V +   + V      I +       +
Sbjct: 386 TESGTVAKSLAFAKNPFKT--------KSGACGTVIRNAEMKVVDTETGISLP-----RN 445

Query: 405 HVGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSG 464
             G I  RG  LM  Y +   A       + W  TGDIG +DD+  +++V R    IK  
Sbjct: 446 KSGEICVRGHQLMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFK 485

Query: 465 GENIYPEEVSLFLL 473
           G  + P E+   L+
Sbjct: 506 GYQVAPAELEALLI 485

BLAST of Lsi01G000800 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 107.5 bits (267), Expect = 4.6e-23
Identity = 108/433 (24.94%), Postives = 182/433 (42.03%), Query Frame = 0

Query: 45  LARGLLDLGLLPGDVVVISAFNSDWYLEWLLAVAFVGGIAAPLNYRWSFEEAISAIVAVQ 104
           +A GL  LG+  GDV++I   NS  ++   +  + +G ++   N  ++ +E    + +  
Sbjct: 88  VASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKS-S 147

Query: 105 PVMLVIDESCCLWYSKLQNDHIPSLRWHVFIGSHLPGYNKTRGALTSEELKKHYVINIPP 164
              L+I  S   +  KL+N  +      +      P        L +++    +   +  
Sbjct: 148 GAKLIITHS--QYVDKLKN--LGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVD- 207

Query: 165 NFLHAPEGVALICFTSGTTGRPKGVAITHSALIVQTLAKV----AIVGYTEDDVYLHTAP 224
                 +  A + F+SGTTG PKGV +TH +LI     +V      +    +DV L   P
Sbjct: 208 ---IGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLP 267

Query: 225 LCHIGGLSSAIA-MLMLGARHILIPKFEVKSAVEAIDLYGVTSLITVPAMMADLVSLIRC 284
           L HI  L+S +   L  GA  +L+ KFE+ + ++ I  + VT    VP ++  L      
Sbjct: 268 LFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAK---- 327

Query: 285 LYLCFVGLIFERFFRERDNGKTRETVKKILNGGGGLPLELIKDAMKFFPRAKLVSAYGMT 344
                             N     +V+ +L+G   L  EL     +  P+A L   YGMT
Sbjct: 328 --------------NPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMT 387

Query: 345 ETCSSLTFLMLCDPMGETSIPHFQSYEHSKCHSVYQPEGVCVGKPAPHIEIKICLDGSSH 404
           E    L+  +      +  IP     +   C +V +   + V     H+E ++ L G + 
Sbjct: 388 EAGPVLSMSL---GFAKEPIP----TKSGSCGTVVRNAELKV----VHLETRLSL-GYNQ 447

Query: 405 VGRILTRGPHLMLRYYDKISARPSNDVNEVWFDTGDIGSIDDNGNLWLVGRSNGRIKSGG 464
            G I  RG  +M  Y +   A  +    E W  TGDIG +D++  +++V R    IK  G
Sbjct: 448 PGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKG 481

Query: 465 ENIYPEEVSLFLL 473
             + P E+   L+
Sbjct: 508 FQVPPAELESLLI 481