Lsi01G000620 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G000620
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Fatty acid 2-hydroxylase
Location	chr01: 684270 .. 688225 (-)
RNA-Seq Expression	Lsi01G000620
Synteny	Lsi01G000620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGAATGGAAATTGGAGGCATTGTGAATATGTGGGGCTGACGGCTGAGCTACGATTTCGATTCCAAAGGAGTGGGTGCTTATCAACCTTCCAATTCTAACATAAAAACCCTCCTTTTTCCTTCTCTAATCTTCGGCTTTCCCTTTTTCTTTGTCTCTTACATTTCCCTCTGAATATTCCTTCAGATCTCGTGATACGGCTGGAGGAGGACTTTAATCATCTTCCTTCTCTATCCTCCTCTTGGGTTTCTCCATCTCCATCCCCATTGCATTGCATTGTGCCAAAAAGGTTAGTATTCGGGGGATTCTTCTTACCCATCTTCAATTTCTTCTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAATCGAGTCAGTGGGGGTGGATATTTGAATTTCTCGATTTCTTTATTTAATTCCTCTTCTGGGTATTGTTCTTCAATGTCTTAAGTCACTAGAATCTGTTCCCCACTCTAGTTCATTATGTGATGTTGTGCTAATTGGGATGCCGTTGTCTTGCTTTCTTCTTTTTTTTTTAGATTAATCCTCTTCTGGGTATTCTCTATCTTCTTTCTTAAATCTCTTGGTTTAGGTTAGCACGTTTTGGGTCCTCCAATCCAATGGGGGCATATTTTCAGATTATGCTTAGGAAAACTACAATTTGGTCCTTTACTTTGAGCAATATTTCACCTCAGTTTCCAAGGTTATGATTAGAAGATGAGATTTTAGTCTTTTTGGATTTGAATTGATCTGCCATTCATGAAAATCTAATCATTCCGATTTGGCAAGTTAAATGGTGATGTTGCTTTGCCTGGAGCATTAAGTTTGAGAAATATATAATTCTGCCTTTACTCATATAGCCTCTTTTCGCTTAATTAACTGAACCTGTCACAACCCATAGGATTGGCAAAGTCACATCATCGTTTAGTTTGTTTAGTTTAGTCAATTACGGAATAATGATTTTAGAGACCAGTTTCAAACATTTCTCAGATCTCTGTAGTTAAGATTTCGCTTTTTAGAATTAGGTGACAAAAAATAAACATTTCTCTTATATATTTATTAATGAAATAGGAAATAGGAAATGATTCCAATATTTTAAATAAGTATAAGGAAGTGCTATTCAGTTAACTTGACTGCATGAAACTGTTGTGCTGTGAGATTTAAGTAACAAAATACAATTCGTTTTTCCTTAAACCTGTGGGTATTAGTGCAATCAATCTCATATACTCTGTTGTAAACTTTCTTGTCTGCCCAAGTGCATTTCTATCTGTGCTGAATAAAAGTCCACTTCTTTTAACAAACTTGCATGTTTTAATGAGTTTGGTGGTAAAGTTATAAGCCAATAGTTTAAAGTAATTTTATTCAGTTATCTAATTCAGTTATCTTATTTAAGCAGTTGAAAGTCAATTGAATCGTCTGTTTTTAATTGTACCGTTATGTTTGTTTGCACTTACAAACGAGGTTTATTTTCTTACTGTTGACTAACTTCCGTATTGTTCATCTTCCCTTCTGCAAAAGGTGAAATTCAAAACCACTCAGTAAGAAGATTCCAAGATGGCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTCGTTTTCCAGGTTAGCCTCCTCCTTTTCTCCTCAATACATATTCTGTTATTTCCTGCAAAATTGATATCATACACATCATAGGAACCGAGCTTTCAAATGCAAATAAGTCACTGTAATTTAGGCTCTTAAAGTTCTAATCCTTTTAAAATCTCCCTACCTTTATGTTTACAAGGGCAAGAAGGATAGGTGTAAGTCTCGTCTCGATTACATTTAATCTATGCTAAGTTGCTTGGTTGTACTCTTCTTAGTTGAATTATAATCTTCTCTGCCATGTAGTCTTCTTTTAACGTTTCTAACTAATGAAATTTTTACAGGTTGGCCATCTTGGAGCAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGAGGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGGTTTTACTTCGCTTTTTCGTATTTATACCGTCATACAACCTTAAGAATGAAGATAAACACAATATTAAATGGTCATAGGTGCTGAAACTACTAATGGATAAACTCTTTTTGTGGTCATACTAGCAATTTTCATCTAATAATTTGCTCATTAATAATATGCTGCGGTGATTTTTGAAATTTTGCTCACAATTTGAAATCTCAATGAAGAAGGGTATACTCGACCCTTCAGATAATAGCAACGCCTCTACTTGGAGATCAATCTGTAATATCTTTTCCCTTGGGACAAATAATTTATCATATCCTGTGCTTAGTACTATGGAGGTGTCATCAAATAAGTCACCATGCATACATAGATGTGATAGTTGATATATAACTTTTCTGAAATGACTAATGAGAGGCTTGCCTTTAATTTCTTTGTAATAACAGATGTTGACCCGCACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCAAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGGTTTTCATATGGACTTTGCTTGAGTACACATTGCACCGCTTTCTTTTCCACATGGAAACAATAAGCTATTGGTACTCTCTCTTTCTATCTCTCTCACACACAGATGAATGGTGAATTCAAGGACACTGATGATGTCACAACCTGTTTCAGGGGAAACACTCTACACTATCTACTTCATGGTTGTCATCACAAGCATCCAATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACCATCTTAAGTGTGCCGGTAAGTTCCAAAAATGGTTACCTCCGAGATTATCCTCTGCAAATTTGTATTCAAGGGACAATCATGTGCTGCTCATTGTTGTATTTCAATTGAGCAAATTCTTTTCGTTGCAATTTACGTTAATGAATCGAAATGCCTCATGCTATGAACTAATGATGTGCACTGCTCCATGGGACTGGCCAAAACGTTGGTTCTTTATGCAAAATTAGCTTGAGTGAACTACAAGTGTCATATAGAAATTGTCAACGAAATAATCTGTATAATTTTGTGCAATCTAAATAACCAATCAAGTTCTGGGCTAATGTCAATAACTCAAGGGATGGTATGGTTTCTTCTCTCCTTTCTTATGGAGCTGTCTTTGTGATTTGTGTCTGTTAGCTGTGGATCGTCATCAGACTTACATCGACTCCAGCGGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCTTCAGGGTTATCTCAAAATTTGAAGGTAAAGTGGAAGCATAAGACTGTTTATGATGTGTTTTTTGAGAATTGAGAAGGAAAGATTTCTTATGATTGTTACGATGTGTTGAACAGAGATATCACTTGAACCACCACTTCAGAGTTCAAAGCGAGGGATTTGGTATCACATCTCCACTATGGGACAGAGCATTTGGAACATATCCTACAACTAAACCCATTCAGAAAAGCAAATGATGATGTTGGTTGCACACATATTATGTCGTCACCAGATTTATGTATTGAGTTGGTTCTTCCTTTTTTCTTCTGTACATTGAGCACCAAATTCACGCTTTGACAACCATTATATATATTTTGGGTGATAATCTGGCTTTGCTTGAATACCCTACTGTTCTTTCTTCCCTCCCTCTGGTTATCAGATGTAGTAAGCTATTTTCATGTACATTAGCTATGGAGCTACACTTCAATGAGTATTATGTTCTTTAATTTGCAAATTGCGAAGCTGCTATGTTTCTCTGTAATCTGTTTTTCATGCAGAAAGAAAAACACACACACATTACACACCACCATTGAGTAATAAATTACCAACAAGGTTATGAGATTTGTTTAATTATCAATGTTGTTTACTTGTATTGACACTTGTG

mRNA sequence

CCGAATGGAAATTGGAGGCATTGTGAATATGTGGGGCTGACGGCTGAGCTACGATTTCGATTCCAAAGGAATCTCGTGATACGGCTGGAGGAGGACTTTAATCATCTTCCTTCTCTATCCTCCTCTTGGGTTTCTCCATCTCCATCCCCATTGCATTGCATTGTGCCAAAAAGGTGAAATTCAAAACCACTCAGTAAGAAGATTCCAAGATGGCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTCGTTTTCCAGGTTGGCCATCTTGGAGCAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGAGGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGATGTTGACCCGCACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCAAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGATGAATGGTGAATTCAAGGACACTGATGATGTCACAACCTGTTTCAGGGGAAACACTCTACACTATCTACTTCATGGTTGTCATCACAAGCATCCAATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACCATCTTAAGTGTGCCGTTCCAAAAATGGTTACCTCCGAGATTATCCTCTGCAAATTTGTATTCAAGGGACAATCATGTGCTGCTCATTGTTGTATTTCAATTGAGCAAATTCTTTTCGTTGCAATTTACGTTAATGAATCGAAATGCCTCATGCTATGAACTAATGATGTGCACTGCTCCATGGGACTGGCCAAAACAAATTGTCAACGAAATAATCTGTATAATTTTGTGCAATCTAAATAACCAATCAAGTTCTGGGCTAATGTCAATAACTCAAGGGATGCTGTGGATCGTCATCAGACTTACATCGACTCCAGCGGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCTTCAGGGTTATCTCAAAATTTGAAGGTAAAGTGGAAGCATAAGACTGTTTATGATAGATATCACTTGAACCACCACTTCAGAGTTCAAAGCGAGGGATTTGGTATCACATCTCCACTATGGGACAGAGCATTTGGAACATATCCTACAACTAAACCCATTCAGAAAAGCAAATGATGATGTTGGTTGCACACATATTATGTCGTCACCAGATTTATGTATTGAGTTGGTTCTTCCTTTTTTCTTCTGTACATTGAGCACCAAATTCACGCTTTGACAACCATTATATATATTTTGGGTGATAATCTGGCTTTGCTTGAATACCCTACTGTTCTTTCTTCCCTCCCTCTGGTTATCAGATGTAGTAAGCTATTTTCATGTACATTAGCTATGGAGCTACACTTCAATGAGTATTATGTTCTTTAATTTGCAAATTGCGAAGCTGCTATGTTTCTCTGTAATCTGTTTTTCATGCAGAAAGAAAAACACACACACATTACACACCACCATTGAGTAATAAATTACCAACAAGGTTATGAGATTTGTTTAATTATCAATGTTGTTTACTTGTATTGACACTTGTG

Coding sequence (CDS)

ATGGCTTCAAAGCCTTTTACTGTAGATTTAAACAAGCCTCTCGTTTTCCAGGTTGGCCATCTTGGAGCAGCTTACAATGAATGGGTTCACCAGCCTATAATTAGCAAGGAGGGCCCTCGATTCTTCGGAAATGATTTTCTGGAGATGTTGACCCGCACAGTATGGTGGGTAATTCCACTTGTTTGGCTGCCAGTGATTTCCTGGTTGGTTTCTGTGTCTTTGTCAAGGGGCCTTACTCCCTCTGATGCAGCTTCATGTTTGGCTGGTGGGATGAATGGTGAATTCAAGGACACTGATGATGTCACAACCTGTTTCAGGGGAAACACTCTACACTATCTACTTCATGGTTGTCATCACAAGCATCCAATGGATGGACTCAGACTCGTCTTCCCACCTGCTGCAACAACCATCTTAAGTGTGCCGTTCCAAAAATGGTTACCTCCGAGATTATCCTCTGCAAATTTGTATTCAAGGGACAATCATGTGCTGCTCATTGTTGTATTTCAATTGAGCAAATTCTTTTCGTTGCAATTTACGTTAATGAATCGAAATGCCTCATGCTATGAACTAATGATGTGCACTGCTCCATGGGACTGGCCAAAACAAATTGTCAACGAAATAATCTGTATAATTTTGTGCAATCTAAATAACCAATCAAGTTCTGGGCTAATGTCAATAACTCAAGGGATGCTGTGGATCGTCATCAGACTTACATCGACTCCAGCGGTAGCTCCTGCTTTGTTTGGAGGTGGGTTGCTGGGATATGTAATGTACGACGTAACTCATTATTACCTGCATCATGGAAAGCCATCTTCAGGGTTATCTCAAAATTTGAAGGTAAAGTGGAAGCATAAGACTGTTTATGATAGATATCACTTGAACCACCACTTCAGAGTTCAAAGCGAGGGATTTGGTATCACATCTCCACTATGGGACAGAGCATTTGGAACATATCCTACAACTAAACCCATTCAGAAAAGCAAATGA

Protein sequence

MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPLVWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDTDDVTTCFRGNTLHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK

Homology

[Close]

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot
Match: O48916 (Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN=FAH1 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 141/336 (41.96%), Postives = 170/336 (50.60%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ FTVDL KPLVFQVGHLG  Y EWVHQPI +KEGPRFF +DF E LT TVWW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  VWLPVISWLVSVSLSRGLT-PSDAASCLAGGMNGEFKD-------TDDVTTCFRGNTLHY 120
           +WLPV+ W +S S+S G + P      + G     F +           T  + GNT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           L+HGCHHKHPMD LRLVFPP AT IL  PF                              
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPF------------------------------ 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                      W
Sbjct: 181 -----------------------------------------------------------W 237

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
            + +  STP+ APALFGGG+LGYVMYDVTHYYLHH +P+  +++NLK          +YH
Sbjct: 241 NIAKAISTPSTAPALFGGGMLGYVMYDVTHYYLHHAQPTRPVTKNLK----------KYH 237

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           LNHHFR+Q +GFGITS LWD  FGT PTTK  +K +
Sbjct: 301 LNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPRKEQ 237

[Close]

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot
Match: Q9SUC5 (Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN=FAH2 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.9e-65
Identity = 137/334 (41.02%), Postives = 168/334 (50.30%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ +TVDLNKPLVFQVGHLG  Y EW+HQPI+  EGPRFF +DF E LTRTVWW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNG----EFKDTDDV----TTCFRGNTLHY 120
           +WLPV+ +++S+S S+GLT       +A G+      E+     +    T  +  NT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           LLHGCHHKHP DGLRLVFPP AT IL VP                               
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVP------------------------------- 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                     LW
Sbjct: 181 ----------------------------------------------------------LW 235

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
            ++ L +TPA APA+ GG L GYVMYD+THYYLHHG+P     ++LK          +YH
Sbjct: 241 KLLHLLATPATAPAILGGILFGYVMYDITHYYLHHGQPKEPTFKHLK----------KYH 235

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQK 327
           LNHHFR+Q +G+GITS LWD+ FGT P  K   K
Sbjct: 301 LNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAK 235

[Close]

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot
Match: Q4R4P4 (Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.5e-29
Identity = 98/350 (28.00%), Postives = 136/350 (38.86%), Query Frame = 0


Query: 8   VDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLG  Y+EWVHQP+      R F +D +E L++TVW+ +P++W+P++ 
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--TRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVL 183

Query: 68  WLV----------SVSLSRGLTPSDAASCLAGGMNGEFK------------------DTD 127
           +L           +V L    T   A +       G F                      
Sbjct: 184 YLSWSYYRTFAQGNVRLFTSFTTEYALAVPKSMFPGLFMLGIFLWSLIEYLIHRFLFHMK 243

Query: 128 DVTTCFRGNTLHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRD 187
             +  +    LH+++HG HHK P DG RLVFPP       VP                  
Sbjct: 244 PPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPP-------VP------------------ 303

Query: 188 NHVLLIVVFQLSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQS 247
                                                                       
Sbjct: 304 ------------------------------------------------------------ 363

Query: 248 SSGLMSITQGMLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSG-LSQNL 307
                S+  G+ ++ ++L    AV   +F GGLLGYV+YD+THYYLH G P  G    NL
Sbjct: 364 ----ASLVIGVFYLCLQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHRGSYLYNL 372

Query: 308 KVKWKHKTVYDRYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           K           +H+ HHF  Q  GFGI++ LWD  F T    KP  K++
Sbjct: 424 KA----------HHVKHHFAHQKSGFGISTKLWDYCFHTLIPEKPHLKTQ 372

[Close]

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot
Match: Q2LAM0 (Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-28
Identity = 94/340 (27.65%), Postives = 131/340 (38.53%), Query Frame = 0


Query: 8   VDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLG  Y+EWVHQP+      R F +D +E  ++TVW+ +P++W+P++ 
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPV--ARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVL 183

Query: 68  WLVSVSLSRGLTPSDAASCLAGGMNGEFKDTDDV-------------------------- 127
           +L S S  R LT  +     +   +      + V                          
Sbjct: 184 YL-SWSYYRTLTQDNIRLFASFTRDYSLVVPESVFIGLFVLGMLIWTLVEYLIHRFLFHM 243

Query: 128 ---TTCFRGNTLHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSR 187
              +       LH+++HG HHK P DG RLVFPP   ++                     
Sbjct: 244 KPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASV--------------------- 303

Query: 188 DNHVLLIVVFQLSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQ 247
                 +V F                                                  
Sbjct: 304 ------VVAF-------------------------------------------------- 361

Query: 248 SSSGLMSITQGMLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSG-LSQN 307
                        ++ +RL    AVA  LF GGLLGYV+YD+THYYLH G P  G    N
Sbjct: 364 ------------FYVFLRLILPEAVAGILFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYN 361

Query: 308 LKVKWKHKTVYDRYHLNHHFRVQSEGFGITSPLWDRAFGT 318
           +K           +H+ HHF  Q  GFGI++ LWD  F T
Sbjct: 424 MKA----------HHVKHHFEYQKSGFGISTKLWDYFFHT 361

[Close]

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot
Match: Q7L5A8 (Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 96/349 (27.51%), Postives = 136/349 (38.97%), Query Frame = 0


Query: 8   VDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPLVWLPVIS 67
           VD  KPL++QVGHLG  Y+EWVHQP+      R F +D +E L++TVW+ +P++W+P++ 
Sbjct: 124 VDWRKPLLWQVGHLGEKYDEWVHQPV--TRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVL 183

Query: 68  WL--------------VSVSLSRGLTPSDAASCLAG-GMNGEFK-------------DTD 127
           +L              +  S +   T +   S   G  M G F                 
Sbjct: 184 YLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHMK 243

Query: 128 DVTTCFRGNTLHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRD 187
             +  +    LH+++HG HHK P DG RLVFPP       VP                  
Sbjct: 244 PPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPP-------VP------------------ 303

Query: 188 NHVLLIVVFQLSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQS 247
                                                                       
Sbjct: 304 ------------------------------------------------------------ 363

Query: 248 SSGLMSITQGMLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLK 307
                S+  G+ ++ ++L    AV   +F GGLLGYV+YD+THYYLH G P  G      
Sbjct: 364 ----ASLVIGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKG------ 372

Query: 308 VKWKHKTVYDRYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
               +      +H+ HHF  Q  GFGI++ LWD  F T    KP  K++
Sbjct: 424 ---SYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFHTLTPEKPHLKTQ 372

[Close]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL
Match: A0A5A7U993 (Dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009740 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 4.0e-102
Identity = 209/339 (61.65%), Postives = 212/339 (62.54%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASKPFTVDLNKPLVFQVGHLG AYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T  +  NT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGI---FIWTLLEYTLHRFLFHMETRSYWANT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPS GLSQNLK          
Sbjct: 241 -LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 301 RYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

[Close]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL
Match: A0A1S3AZN1 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo OX=3656 GN=LOC103484300 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 4.0e-102
Identity = 209/339 (61.65%), Postives = 212/339 (62.54%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASKPFTVDLNKPLVFQVGHLG AYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T  +  NT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGI---FIWTLLEYTLHRFLFHMETRSYWANT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPS GLSQNLK          
Sbjct: 241 -LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 301 RYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

[Close]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL
Match: A0A0A0KKX5 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525640 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.4e-101
Identity = 206/339 (60.77%), Postives = 212/339 (62.54%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLG AYNEWVHQPIISK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T  +  NT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGI---FIWTLLEYTLHRFLFHMETRSYWANT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHY+LHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYVLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPS GLSQNLK          
Sbjct: 241 -LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 301 RYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

[Close]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL
Match: A0A6J1ECD2 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111433035 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.7e-100
Identity = 201/336 (59.82%), Postives = 213/336 (63.39%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASK FTVDLNKPLVFQVGHLG AYN+WVHQPI+SKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKTFTVDLNKPLVFQVGHLGEAYNKWVHQPIVSKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMN--GEFKDT------DDVTTCFRGNTLHY 120
           VWLPVISWLVSVSLSRGLTPSD ASCLA G+      + T         T  + GNTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDVASCLAAGVFIWSLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           LLHGCHHKHPMDGLRLVFPPAAT ILSVP                               
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATAILSVP------------------------------- 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                     LW
Sbjct: 181 ----------------------------------------------------------LW 237

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
           +VIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKP+SGLSQNLK          RYH
Sbjct: 241 VVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPTSGLSQNLK----------RYH 237

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           LNHHFR+QS+GFGITSPLWDRAFGTYP+TKPIQ++K
Sbjct: 301 LNHHFRIQSQGFGITSPLWDRAFGTYPSTKPIQENK 237

[Close]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL
Match: A0A6J1D0B7 (dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=3673 GN=LOC111015844 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 8.3e-100
Identity = 200/339 (59.00%), Postives = 210/339 (61.95%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASKP+TVDLNKPLVFQVGHLG AYN+WVHQPI+SKEGPRFFGNDF+EMLTRTVWWVIPL
Sbjct: 1   MASKPYTVDLNKPLVFQVGHLGEAYNQWVHQPIVSKEGPRFFGNDFMEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVI WL SVSLSRGLTPSD ASCLAGG+   F  T              T  + GNT
Sbjct: 61  VWLPVICWLASVSLSRGLTPSDVASCLAGGV---FIWTLLEYTLHRFLFHMETRSYWGNT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHYLLHGCHHKHPMDGLRLVFPP AT ILSVP                            
Sbjct: 121 LHYLLHGCHHKHPMDGLRLVFPPVATAILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAP LFGGGLLGYVMYDVTHYYLHHGKP+SGLSQN+K          
Sbjct: 241 -LWIVIRLTSTPAVAPTLFGGGLLGYVMYDVTHYYLHHGKPTSGLSQNMK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFRVQS+GFGITSPLWDRAFGTYPTTK IQK+K
Sbjct: 301 RYHLNHHFRVQSQGFGITSPLWDRAFGTYPTTKTIQKNK 237

[Close]

BLAST of Lsi01G000620 vs. NCBI nr
Match: XP_038881178.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida] >XP_038881179.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 391.0 bits (1003), Expect = 1.0e-104
Identity = 211/339 (62.24%), Postives = 217/339 (64.01%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASKPFTVDLNKPLVFQVGHLG AYNEWVHQPI+SKEGPRFFGNDF+EMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIVSKEGPRFFGNDFMEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T+ + GNT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGV---FIWTLLEYTLHRFLFHMKTSSYWGNT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRL STPAVAP+LFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLK          
Sbjct: 241 -LWIVIRLASTPAVAPSLFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK
Sbjct: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 237

[Close]

BLAST of Lsi01G000620 vs. NCBI nr
Match: XP_008439533.1 (PREDICTED: dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo] >KAA0052473.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo var. makuwa] >TYK13349.1 dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo var. makuwa])

HSP 1 Score: 381.3 bits (978), Expect = 8.2e-102
Identity = 209/339 (61.65%), Postives = 212/339 (62.54%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASKPFTVDLNKPLVFQVGHLG AYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T  +  NT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGI---FIWTLLEYTLHRFLFHMETRSYWANT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPS GLSQNLK          
Sbjct: 241 -LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 301 RYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

[Close]

BLAST of Lsi01G000620 vs. NCBI nr
Match: XP_023544587.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] >XP_023544588.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.3 bits (970), Expect = 7.0e-101
Identity = 202/336 (60.12%), Postives = 214/336 (63.69%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASK FTVDLNKPLVFQVGHLG AYN+WVHQPI+SKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKTFTVDLNKPLVFQVGHLGEAYNKWVHQPIVSKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMN--GEFKDT------DDVTTCFRGNTLHY 120
           VWLPVISWLVSVSLSRGLTPSD ASCLAGG+      + T         T  + GNTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDVASCLAGGVFIWSLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           LLHGCHHKHPMDGLRLVFPPAAT ILSVP                               
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATAILSVP------------------------------- 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                     LW
Sbjct: 181 ----------------------------------------------------------LW 237

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
           +VIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKP+SGLSQNLK          RYH
Sbjct: 241 VVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPTSGLSQNLK----------RYH 237

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           LNHHFR+QS+GFGITSPLWDRAFGTYP+TKPIQ++K
Sbjct: 301 LNHHFRIQSQGFGITSPLWDRAFGTYPSTKPIQENK 237

[Close]

BLAST of Lsi01G000620 vs. NCBI nr
Match: XP_004134612.1 (dihydroceramide fatty acyl 2-hydroxylase FAH2 [Cucumis sativus] >KGN49479.1 hypothetical protein Csa_003896 [Cucumis sativus])

HSP 1 Score: 377.9 bits (969), Expect = 9.1e-101
Identity = 206/339 (60.77%), Postives = 212/339 (62.54%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M+SKPFTVDLNKPLVFQVGHLG AYNEWVHQPIISK+GPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MSSKPFTVDLNKPLVFQVGHLGEAYNEWVHQPIISKDGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNGEFKDT-----------DDVTTCFRGNT 120
           VWLPVISWLVSVSLSRGLTPSDAASCLAGG+   F  T              T  +  NT
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGI---FIWTLLEYTLHRFLFHMETRSYWANT 120

Query: 121 LHYLLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQ 180
           LHY+LHGCHHKHPMDGLRLVFPPAATTILSVP                            
Sbjct: 121 LHYVLHGCHHKHPMDGLRLVFPPAATTILSVP---------------------------- 180

Query: 181 LSKFFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQG 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 237

Query: 241 MLWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYD 300
            LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPS GLSQNLK          
Sbjct: 241 -LWIVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSPGLSQNLK---------- 237

Query: 301 RYHLNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           RYHLNHHFR QSEGFGITSPLWDRAFGTYPTTK  QK+K
Sbjct: 301 RYHLNHHFRDQSEGFGITSPLWDRAFGTYPTTKSTQKNK 237

[Close]

BLAST of Lsi01G000620 vs. NCBI nr
Match: XP_022925691.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_022925692.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_022925693.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >KAG6581483.1 Dihydroceramide fatty acyl 2-hydroxylase FAH1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034773.1 Dihydroceramide fatty acyl 2-hydroxylase FAH1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 375.9 bits (964), Expect = 3.5e-100
Identity = 201/336 (59.82%), Postives = 213/336 (63.39%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           MASK FTVDLNKPLVFQVGHLG AYN+WVHQPI+SKEGPRFFGNDFLEMLTRTVWWVIPL
Sbjct: 1   MASKTFTVDLNKPLVFQVGHLGEAYNKWVHQPIVSKEGPRFFGNDFLEMLTRTVWWVIPL 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMN--GEFKDT------DDVTTCFRGNTLHY 120
           VWLPVISWLVSVSLSRGLTPSD ASCLA G+      + T         T  + GNTLHY
Sbjct: 61  VWLPVISWLVSVSLSRGLTPSDVASCLAAGVFIWSLLEYTLHRFLFHMETRSYWGNTLHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           LLHGCHHKHPMDGLRLVFPPAAT ILSVP                               
Sbjct: 121 LLHGCHHKHPMDGLRLVFPPAATAILSVP------------------------------- 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                     LW
Sbjct: 181 ----------------------------------------------------------LW 237

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
           +VIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKP+SGLSQNLK          RYH
Sbjct: 241 VVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPTSGLSQNLK----------RYH 237

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           LNHHFR+QS+GFGITSPLWDRAFGTYP+TKPIQ++K
Sbjct: 301 LNHHFRIQSQGFGITSPLWDRAFGTYPSTKPIQENK 237

[Close]

BLAST of Lsi01G000620 vs. TAIR 10
Match: AT2G34770.1 (fatty acid hydroxylase 1 )

HSP 1 Score: 258.5 bits (659), Expect = 7.5e-69
Identity = 141/336 (41.96%), Postives = 170/336 (50.60%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ FTVDL KPLVFQVGHLG  Y EWVHQPI +KEGPRFF +DF E LT TVWW +P+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  VWLPVISWLVSVSLSRGLT-PSDAASCLAGGMNGEFKD-------TDDVTTCFRGNTLHY 120
           +WLPV+ W +S S+S G + P      + G     F +           T  + GNT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           L+HGCHHKHPMD LRLVFPP AT IL  PF                              
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPF------------------------------ 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                      W
Sbjct: 181 -----------------------------------------------------------W 237

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
            + +  STP+ APALFGGG+LGYVMYDVTHYYLHH +P+  +++NLK          +YH
Sbjct: 241 NIAKAISTPSTAPALFGGGMLGYVMYDVTHYYLHHAQPTRPVTKNLK----------KYH 237

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQKSK 329
           LNHHFR+Q +GFGITS LWD  FGT PTTK  +K +
Sbjct: 301 LNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPRKEQ 237

[Close]

BLAST of Lsi01G000620 vs. TAIR 10
Match: AT4G20870.1 (fatty acid hydroxylase 2 )

HSP 1 Score: 250.4 bits (638), Expect = 2.0e-66
Identity = 137/334 (41.02%), Postives = 168/334 (50.30%), Query Frame = 0


Query: 1   MASKPFTVDLNKPLVFQVGHLGAAYNEWVHQPIISKEGPRFFGNDFLEMLTRTVWWVIPL 60
           M ++ +TVDLNKPLVFQVGHLG  Y EW+HQPI+  EGPRFF +DF E LTRTVWW IP 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  VWLPVISWLVSVSLSRGLTPSDAASCLAGGMNG----EFKDTDDV----TTCFRGNTLHY 120
           +WLPV+ +++S+S S+GLT       +A G+      E+     +    T  +  NT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 LLHGCHHKHPMDGLRLVFPPAATTILSVPFQKWLPPRLSSANLYSRDNHVLLIVVFQLSK 180
           LLHGCHHKHP DGLRLVFPP AT IL VP                               
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVP------------------------------- 180

Query: 181 FFSLQFTLMNRNASCYELMMCTAPWDWPKQIVNEIICIILCNLNNQSSSGLMSITQGMLW 240
                                                                     LW
Sbjct: 181 ----------------------------------------------------------LW 235

Query: 241 IVIRLTSTPAVAPALFGGGLLGYVMYDVTHYYLHHGKPSSGLSQNLKVKWKHKTVYDRYH 300
            ++ L +TPA APA+ GG L GYVMYD+THYYLHHG+P     ++LK          +YH
Sbjct: 241 KLLHLLATPATAPAILGGILFGYVMYDITHYYLHHGQPKEPTFKHLK----------KYH 235

Query: 301 LNHHFRVQSEGFGITSPLWDRAFGTYPTTKPIQK 327
           LNHHFR+Q +G+GITS LWD+ FGT P  K   K
Sbjct: 301 LNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAK 235

The following BLAST results are available for this feature:

BLAST of Lsi01G000620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48916	1.1e-67	41.96	Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9SUC5	2.9e-65	41.02	Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q4R4P4	2.5e-29	28.00	Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1	[more]
Q2LAM0	1.3e-28	27.65	Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2	[more]
Q7L5A8	1.6e-28	27.51	Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1	[more]

BLAST of Lsi01G000620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U993	4.0e-102	61.65	Dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3AZN1	4.0e-102	61.65	dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0KKX5	4.4e-101	60.77	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1ECD2	1.7e-100	59.82	dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662...	[more]
A0A6J1D0B7	8.3e-100	59.00	dihydroceramide fatty acyl 2-hydroxylase FAH2-like OS=Momordica charantia OX=367...	[more]

BLAST of Lsi01G000620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881178.1	1.0e-104	62.24	dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X1 [Benincasa hispida...	[more]
XP_008439533.1	8.2e-102	61.65	PREDICTED: dihydroceramide fatty acyl 2-hydroxylase FAH2-like [Cucumis melo] >KA...	[more]
XP_023544587.1	7.0e-101	60.12	dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] ...	[more]
XP_004134612.1	9.1e-101	60.77	dihydroceramide fatty acyl 2-hydroxylase FAH2 [Cucumis sativus] >KGN49479.1 hypo...	[more]
XP_022925691.1	3.5e-100	59.82	dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_0229...	[more]

BLAST of Lsi01G000620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34770.1	7.5e-69	41.96	fatty acid hydroxylase 1	[more]
AT4G20870.1	2.0e-66	41.02	fatty acid hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 229..317 e-value: 5.1E-8 score: 33.3
IPR014430	Sterol desaturase Scs7	PIRSF	PIRSF005149	IPC-B_HD	coord: 221..328 e-value: 6.2E-24 score: 82.7 coord: 94..190 e-value: 1.5E-11 score: 42.0 coord: 2..104 e-value: 1.9E-21 score: 74.5
IPR014430	Sterol desaturase Scs7	PANTHER	PTHR12863	FATTY ACID HYDROXYLASE	coord: 1..144
IPR014430	Sterol desaturase Scs7	PANTHER	PTHR12863	FATTY ACID HYDROXYLASE	coord: 226..328
None	No IPR available	PANTHER	PTHR12863:SF12	DIHYDROCERAMIDE FATTY ACYL 2-HYDROXYLASE FAH1-LIKE	coord: 226..328
None	No IPR available	PANTHER	PTHR12863:SF12	DIHYDROCERAMIDE FATTY ACYL 2-HYDROXYLASE FAH1-LIKE	coord: 1..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G000620.1	Lsi01G000620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0080132	fatty acid alpha-hydroxylase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity