Lsi01G000580 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G000580 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.2e-199
Identity = 348/536 (64.93%), Postives = 422/536 (78.73%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPL--SAPSAAAASLYTTFLHCF-ESNSSPSDGVSAIVFDRQNASYTS 60
           +S F    FL+ LP   SA   ++ S+Y +F+ CF +   SP   ++  VF R N S++S
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 67

Query: 61  VLRAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYI 120
           VLRAYIRN RFNTSS PKP IIVTP  + HV +AV CSK +   LKIRSGGHDYEG+SYI
Sbjct: 68  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 127

Query: 121 SEVQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVG 180
           S+  F ILDMSNLR V+VD+ADQSAW+ AGATLGEVYYRIWEKSKV G+PAGVCPTVGVG
Sbjct: 128 SDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVG 187

Query: 181 GHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVL 240
           GHISGGGYGNMLRK+GLSVD+++DA+IVDV G+ILD +SMGEDLFWAI GGGGASFGVVL
Sbjct: 188 GHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVL 247

Query: 241 AYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKG 300
            YK++LVPVPETVTVFRVE+ ++  A D+V +WQ V P TD NLF+R+L+QPV+   +K 
Sbjct: 248 GYKVKLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVT---RKK 307

Query: 301 TRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SP 360
            +TVRATV+ALFLG++EE+V+LLGKEFPEL L+KENC+EM+W  S +WW N    T   P
Sbjct: 308 VKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDP 367

Query: 361 KALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPST 420
           K  LDRN+D + F KRKSDYV   I RDG++ L+KKM E+GK GLVFNPYGGKM+E+   
Sbjct: 368 KVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVN 427

Query: 421 ATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDL 480
           ATPFPHR+  L+KIQYSV W E   + ++ F+ Q   LYSFMT FVSKNPR ++LNYRD+
Sbjct: 428 ATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDV 487

Query: 481 DIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSKA 532
           DIG N++  NS+E+G VYG KYFG+NF+RLVKVKTA DP+NFF NEQSIPT  SKA
Sbjct: 488 DIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTVLSKA 539

BLAST of Lsi01G000580 vs. ExPASy Swiss-Prot
Match: Q9LPC3 (Berberine bridge enzyme-like 1 OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.0e-193
Identity = 336/535 (62.80%), Postives = 423/535 (79.07%), Query Frame = 0

Query: 8   LFLLLLPLSAPSAAAA----SLYTTFLHCFESNS-SPSDGVSAIVFDRQNASYTSVLRAY 67
           +FL++  L + S A A    S+Y +FL CF + + +P + +  +V  + +AS+T  LRAY
Sbjct: 7   VFLIVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAY 66

Query: 68  IRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEVQF 127
           IRNARFNTS++PKPL+++    E HVQ+ V+C+K +  QLK RSGGHDY+GVSYIS   F
Sbjct: 67  IRNARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPF 126

Query: 128 IILDMSNLRAVTVDVADQ--SAWVQAGATLGEVYYRIWEKSK---VLGYPAGVCPTVGVG 187
            +LDMS LR +TVD++D   SAWV AGATLGEVYY IW+ SK     G+PAGVCPTVG G
Sbjct: 127 FVLDMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVGAG 186

Query: 188 GHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVL 247
           GHISGGGYGNM+RKYGLSVD++ DA+IVDV GRILD +SMGEDLFWAI GGGGASFGV+L
Sbjct: 187 GHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASFGVIL 246

Query: 248 AYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKG 307
           ++KI+LVPVP  VTVFRVE+T+ +NA D+V +WQ VAP T  +LFMRL+LQPV+   +  
Sbjct: 247 SFKIKLVPVPPRVTVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVT---RNT 306

Query: 308 TRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SP 367
           T+TVRA+V+ALFLGK  +L+SLL KEFPELGL+ ENCTEM+WI SV+WWAN D  T   P
Sbjct: 307 TQTVRASVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKP 366

Query: 368 KALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPST 427
           + LLDRN DS+ FLKRKSDYV+K IS+DGLD+L KK++E GK GLVFNPYGGKMSE+ +T
Sbjct: 367 EILLDRNPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATT 426

Query: 428 ATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDL 487
           ATPFPHR   L+K+Q+S+NW +PG+  +  F+++ R  YS+M PFV+KNPR ++LNYRDL
Sbjct: 427 ATPFPHRK-RLFKVQHSMNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDL 486

Query: 488 DIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           DIG N++  NS+ +  VYG KYFGENF+RLVKVKTAVDPENFF +EQSIPT  +K
Sbjct: 487 DIGINSHGPNSYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTK 537

BLAST of Lsi01G000580 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 674.9 bits (1740), Expect = 7.6e-193
Identity = 331/528 (62.69%), Postives = 415/528 (78.60%), Query Frame = 0

Query: 5   LPFLFLLLLPLSAPSAA---AASLYTTFLHCF-ESNSSPSDGVSAIVFDRQNASYTSVLR 64
           +PF F  LL LS P ++   + S+Y +FL CF +   SP   ++  VF + N +++SVLR
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 65  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 124
           AYIRNARFNTSS  KP II+TP  ESHV +AV CSK +   LKIRSGGHDY+G+SYIS+ 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 125 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 184
            F ILDMSN+R V+VD+A  SAW+ AGATLGEVYYRIWEKS+V G+PAGVCPTVGVGGH+
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 185 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 244
           SGGGYGNM+RK+GLSVD++ DA+IVDV GR+LD ++MGEDLFWAI GGGG S+GVVL YK
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 245 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 304
           ++LVPVP  VTVFRVE+ ++  A D+V +WQ V P TD NLFMR+L+QPV+   +K  +T
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVT---RKKVKT 311

Query: 305 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SPKAL 364
           VRA+V+ALFLG+++E+V+LL KEFPELGL+KENC+EM+W  S +WW N    T   PK  
Sbjct: 312 VRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVF 371

Query: 365 LDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATP 424
           LDRN+D+S F KRKSDYV   I + G++ L+KKMIE+GK GLVFNPYGGKM+E+   A P
Sbjct: 372 LDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKP 431

Query: 425 FPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG 484
           FPHR   L+KIQYSVNW E  ++ ++ ++ Q + LYSFMT FVSKNPR S+ NYRD+DIG
Sbjct: 432 FPHR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIG 491

Query: 485 TNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT 527
            N++  NS+++G VYG KYFGENF+RLVK+KTAVDP NFF NEQSIPT
Sbjct: 492 VNDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPT 535

BLAST of Lsi01G000580 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-191
Identity = 332/536 (61.94%), Postives = 417/536 (77.80%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAA---AASLYTTFLHCFESNS-SPSDGVSAIVFDRQNASYT 60
           M  F   LFL+   +S   A      ++Y  FL CF + + +P + ++ +V  +  A++T
Sbjct: 1   MKIFCLILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFT 60

Query: 61  SVLRAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSY 120
            VLRAYIRNARFNT++ PKP I++    ESHVQ+AVIC+K + IQLK RSGGHDYEGVSY
Sbjct: 61  PVLRAYIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSY 120

Query: 121 ISEVQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGV 180
           IS V F +LDMSNLR +TVD A +SAWV AGATLGEVYYRIWEK+K  G+PAGVCPTVG 
Sbjct: 121 ISHVPFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGA 180

Query: 181 GGHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVV 240
           GGHISGGGYGNM+RKYGLSVD++ DA+IVDV G++LD + MGED+FWAI GGGGASFGV+
Sbjct: 181 GGHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVI 240

Query: 241 LAYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKK 300
           LA+KI+LVPVP TVTVFRVE+ + +NA ++V +WQ VAP TD  LFMRLLLQPV+   + 
Sbjct: 241 LAFKIKLVPVPPTVTVFRVEKNLVENATEMVHKWQFVAPKTDPGLFMRLLLQPVT---RN 300

Query: 301 GTRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--S 360
             +TVRA+V+ALFLG    ++S+L KEFPELGL+KENCTEM+WI SV+WWAN D  T   
Sbjct: 301 KMQTVRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIK 360

Query: 361 PKALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPS 420
           P+ LLDRN D + F KRKSD+V+K I++DGLD+L+KKMIE+GK GLVFNPYGG MS + +
Sbjct: 361 PEILLDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVAT 420

Query: 421 TATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRD 480
           T TPFPHR   LYKIQ+S+NW +PG++A+  F+++ +  YS+M PFV+KNPR +++NYRD
Sbjct: 421 TKTPFPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRD 480

Query: 481 LDIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           LDIG N    NS+    V+G  YFGENF+RLVKVKTAVDP+NFF +EQSIPT   K
Sbjct: 481 LDIGVNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLPGK 532

BLAST of Lsi01G000580 vs. ExPASy Swiss-Prot
Match: Q9SA89 (Berberine bridge enzyme-like 12 OS=Arabidopsis thaliana OX=3702 GN=At1g30740 PE=2 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 5.5e-175
Identity = 303/528 (57.39%), Postives = 406/528 (76.89%), Query Frame = 0

Query: 4   FLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSD-GVSAIVFDRQNASYTSVLRAY 63
           FL F F     +S  SA + ++Y  F+ CF++ ++ SD  +S +V  R + S+T  LRAY
Sbjct: 5   FLLF-FAASYSMSLSSADSVTIYEDFVQCFKNVTTISDIDLSDVVLPRTSISFTPTLRAY 64

Query: 64  IRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEVQF 123
           IRNARFNTSS PKP II+ P ++SHVQ+AVIC+K + +QLKIRSGGHDY+G+SY+S V F
Sbjct: 65  IRNARFNTSSMPKPSIIIVPRVDSHVQAAVICAKTLNLQLKIRSGGHDYDGLSYVSAVTF 124

Query: 124 IILDMSNLRAVTVDVAD--QSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 183
           ++LD+SN R +TVD+ D   SAWVQ GATLGE+YYRIWEKS+V  +PAGVCPTVGVGGH+
Sbjct: 125 LVLDLSNFRNITVDLNDGGGSAWVQTGATLGELYYRIWEKSEVHAFPAGVCPTVGVGGHV 184

Query: 184 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 243
           SGGGYG+M+RK+GL++DH++DA IVD  G+I D +SM EDLFWAIRGGGG SFGVVLA+K
Sbjct: 185 SGGGYGHMIRKFGLTIDHVVDATIVDANGQIHDRKSMEEDLFWAIRGGGGGSFGVVLAFK 244

Query: 244 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 303
           ++LV VP+TVTVFRV++++++NA D+V +WQ VAP TD  LFMR+LL   SS  +  T T
Sbjct: 245 VKLVTVPKTVTVFRVDKSVDENALDMVYKWQFVAPRTDPGLFMRVLL---SSPTQNKTST 304

Query: 304 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWAN-FDIG-TSPKAL 363
           V   + AL+LGK++++V  + +EFPELGL+KE+C EM+WI S++WW N  D+    P+ L
Sbjct: 305 VNTKLRALYLGKADDVVLKMAEEFPELGLKKEDCKEMTWIQSLLWWMNHVDVDKVKPEIL 364

Query: 364 LDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATP 423
           L+R  DS+ FLKRKSDYV+K +++  L+ L++K+  + +TGLV NPYGG ++     AT 
Sbjct: 365 LEREPDSAKFLKRKSDYVEKEMTKPELNRLFQKLATLDRTGLVLNPYGGSLNVTAVNATA 424

Query: 424 FPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG 483
           FPHR   LYKIQ+SV W + G +A++ ++  +R  Y+ MTPFVSKNPR S+LNYRD+DIG
Sbjct: 425 FPHR-HKLYKIQHSVTWPDAGPEAERLYIGNLRTTYNIMTPFVSKNPRSSYLNYRDIDIG 484

Query: 484 TNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT 527
            N++  + +  G +YG KYFGENF+RLV+VKTAVDP+NFF NEQSIPT
Sbjct: 485 VNDHGADGYRKGEIYGRKYFGENFDRLVRVKTAVDPDNFFRNEQSIPT 527

BLAST of Lsi01G000580 vs. ExPASy TrEMBL
Match: A0A1S3AYZ9 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484297 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 486/530 (91.70%), Postives = 513/530 (96.79%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP S P+AAA S+YTTFLHCFESNS+PS GVSAIVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSTPTAAADSVYTTFLHCFESNSNPSAGVSAIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FI+LDMSNLRAVTVDVADQSAWV+AGA LGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIVLDMSNLRAVTVDVADQSAWVEAGAILGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGLSVDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLSVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RATVIALFLGKSEELVSLL KEFPELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRATVIALFLGKSEELVSLLKKEFPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNWDEPG +ADQ+FVKQIRRLYSFMTPFV+KNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWDEPGPEADQQFVKQIRRLYSFMTPFVTKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT SSK
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTLSSK 530

BLAST of Lsi01G000580 vs. ExPASy TrEMBL
Match: A0A5A7UG36 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009790 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.8e-285
Identity = 485/530 (91.51%), Postives = 513/530 (96.79%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP S P+AAA S+YTTFLHCFESNS+PS GVSAIVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSTPTAAADSVYTTFLHCFESNSNPSAGVSAIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FI+LDMSNLRAVTVDVADQSAWV+AGA LGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIVLDMSNLRAVTVDVADQSAWVEAGAILGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGLSVDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLSVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RATVIALFLGKSEELVSLL K+FPELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRATVIALFLGKSEELVSLLKKKFPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNWDEPG +ADQ+FVKQIRRLYSFMTPFV+KNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWDEPGPEADQQFVKQIRRLYSFMTPFVTKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT SSK
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTLSSK 530

BLAST of Lsi01G000580 vs. ExPASy TrEMBL
Match: A0A0A0KM88 (FAD-binding PCMH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525670 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.5e-281
Identity = 479/530 (90.38%), Postives = 510/530 (96.23%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP SAP+AAA S+Y+TFLHCF+SNS+ S GVS+IVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSAPTAAADSVYSTFLHCFQSNSNSSAGVSSIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FIILDMSNLR VTVDVADQSAWV AGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIILDMSNLRTVTVDVADQSAWVGAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGL+VDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLAVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RA+V+ALFLGKSEELVSLL KE PELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRASVVALFLGKSEELVSLLKKELPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGL+WLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLNWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNW+EPG +ADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWNEPGPEADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT S K
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTHSRK 530

BLAST of Lsi01G000580 vs. ExPASy TrEMBL
Match: A0A6J1ECY1 (berberine bridge enzyme-like 21 OS=Cucurbita moschata OX=3662 GN=LOC111433036 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.7e-276
Identity = 475/529 (89.79%), Postives = 503/529 (95.09%), Query Frame = 0

Query: 1   MSPFLP-FLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVL 60
           MS  LP  LFL+LL  S PSAAA S+YT FL CF+SNS  S+GVS IVF RQNASYTSVL
Sbjct: 39  MSRLLPLLLFLILLRFSPPSAAADSVYTQFLQCFQSNSDTSNGVSDIVFSRQNASYTSVL 98

Query: 61  RAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISE 120
           RAYIRNARFNTSSAPKP+IIVTP  ESHVQSAVICSKK+GIQLKIRSGGHDYEG+SY+S+
Sbjct: 99  RAYIRNARFNTSSAPKPVIIVTPFTESHVQSAVICSKKLGIQLKIRSGGHDYEGISYVSD 158

Query: 121 VQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGH 180
           V+FI+LDMSNLRAVTVDVAD+SAWVQAGATLGEVYY IWEKSKVLGYPAG+CPTVGVGGH
Sbjct: 159 VEFIVLDMSNLRAVTVDVADKSAWVQAGATLGEVYYGIWEKSKVLGYPAGICPTVGVGGH 218

Query: 181 ISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAY 240
           ISGGGYGNMLRKYGLSVD ILDARIVDVKGRILDS+SMGEDLFWAI GGGGASFGVVLAY
Sbjct: 219 ISGGGYGNMLRKYGLSVDQILDARIVDVKGRILDSKSMGEDLFWAISGGGGASFGVVLAY 278

Query: 241 KIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR 300
           KIRLVPVPETVTVFRVERT+EQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR
Sbjct: 279 KIRLVPVPETVTVFRVERTLEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR 338

Query: 301 TVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALL 360
           TVRA+V+ALFLGKSEE+VSLLGKEFPELGLQKENC E+SWIDSV+WW NFD GTSPKALL
Sbjct: 339 TVRASVVALFLGKSEEVVSLLGKEFPELGLQKENCIELSWIDSVLWWGNFDNGTSPKALL 398

Query: 361 DRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF 420
           DRN+DSSGFL+RKSDYVQ PISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF
Sbjct: 399 DRNLDSSGFLRRKSDYVQTPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF 458

Query: 421 PHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGT 480
           PHRAGNLYKIQYSVNW+EPG +ADQEFVKQIRRLYS+MTPFVSKNPRQSFLNYRDLDIGT
Sbjct: 459 PHRAGNLYKIQYSVNWNEPGPEADQEFVKQIRRLYSYMTPFVSKNPRQSFLNYRDLDIGT 518

Query: 481 NNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRS 529
           NNN+KNSFEDG+VYG KYFGENF+RLVKVKTAVDPENFFWNEQSIPT S
Sbjct: 519 NNNNKNSFEDGKVYGLKYFGENFDRLVKVKTAVDPENFFWNEQSIPTLS 567

BLAST of Lsi01G000580 vs. ExPASy TrEMBL
Match: A0A6J1IMG1 (berberine bridge enzyme-like 21 OS=Cucurbita maxima OX=3661 GN=LOC111477814 PE=3 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 4.1e-274
Identity = 472/529 (89.22%), Postives = 501/529 (94.71%), Query Frame = 0

Query: 1   MSPFLP-FLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVL 60
           MS  LP  LFL+LL  S PSAAA S+Y+ FL CF+SNS  S GVS IVF  QNASYTSVL
Sbjct: 39  MSRLLPLLLFLILLRFSPPSAAADSVYSQFLQCFQSNSDTSRGVSDIVFSPQNASYTSVL 98

Query: 61  RAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISE 120
           RAYIRNARFNTSSAPKP+IIVTP  ESHVQSAVICSKK+GIQLKIRSGGHDYEG+SY+S+
Sbjct: 99  RAYIRNARFNTSSAPKPVIIVTPFTESHVQSAVICSKKLGIQLKIRSGGHDYEGISYVSD 158

Query: 121 VQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGH 180
           V+FI+LDMSNLRAVTVDVAD+SAWVQAGATLGEVYY IWEKSKVLGYPAG+CPTVGVGGH
Sbjct: 159 VEFIVLDMSNLRAVTVDVADKSAWVQAGATLGEVYYGIWEKSKVLGYPAGICPTVGVGGH 218

Query: 181 ISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAY 240
           ISGGGYGNMLRKYGLSVD ILDARIVDVKGRILDS+SMG+DLFWAI GGGGASFGVVLAY
Sbjct: 219 ISGGGYGNMLRKYGLSVDQILDARIVDVKGRILDSKSMGKDLFWAISGGGGASFGVVLAY 278

Query: 241 KIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR 300
           KIRLVPVPETVTVFRVERT+EQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR
Sbjct: 279 KIRLVPVPETVTVFRVERTLEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTR 338

Query: 301 TVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALL 360
           TVRA+V+ALFLGKSEE+VSLLGKEFPELGLQKENC E+SWIDSV+WW NFD GTSPKALL
Sbjct: 339 TVRASVVALFLGKSEEVVSLLGKEFPELGLQKENCIELSWIDSVLWWGNFDNGTSPKALL 398

Query: 361 DRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF 420
           DRN+DSSGFL+RKSDYVQ PISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF
Sbjct: 399 DRNLDSSGFLRRKSDYVQTPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPF 458

Query: 421 PHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGT 480
           PHRAGNLYKIQYSVNW+EPG +ADQEFVKQIRRLYS+MTPFVSKNPRQSFLNYRDLDIGT
Sbjct: 459 PHRAGNLYKIQYSVNWNEPGPEADQEFVKQIRRLYSYMTPFVSKNPRQSFLNYRDLDIGT 518

Query: 481 NNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRS 529
           NNNDKNSFEDG+VYG KYFGENF+RLVKVKTAVDP+NFFWNEQSIPT S
Sbjct: 519 NNNDKNSFEDGKVYGLKYFGENFDRLVKVKTAVDPDNFFWNEQSIPTLS 567

BLAST of Lsi01G000580 vs. NCBI nr
Match: XP_038881763.1 (berberine bridge enzyme-like 21 [Benincasa hispida])

HSP 1 Score: 1007.7 bits (2604), Expect = 3.8e-290
Identity = 499/528 (94.51%), Postives = 516/528 (97.73%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           ++PF+P L LLLLP SAPSAAA S+YTTFLHCFESNS+PSDGVSAIVF R+NASYTSVLR
Sbjct: 3   LNPFIPLLLLLLLPFSAPSAAADSVYTTFLHCFESNSNPSDGVSAIVFARENASYTSVLR 62

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKPLIIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYISEV
Sbjct: 63  AYIRNARFNTSSAPKPLIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISEV 122

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 123 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 182

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGL+VDHILDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 183 SGGGYGNMLRKYGLTVDHILDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 242

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT
Sbjct: 243 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 302

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSV+WW NFDIGTSPKALLD
Sbjct: 303 IRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVLWWGNFDIGTSPKALLD 362

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RNIDS+GFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPST TPFP
Sbjct: 363 RNIDSAGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTETPFP 422

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNW+E G +ADQEFVKQIRRLYS+MTPFVSKNPRQSFLNYRDLDIG N
Sbjct: 423 HRAGNLYKIQYSVNWNEAGPEADQEFVKQIRRLYSYMTPFVSKNPRQSFLNYRDLDIGIN 482

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRS 529
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT S
Sbjct: 483 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTLS 530

BLAST of Lsi01G000580 vs. NCBI nr
Match: XP_008439529.2 (PREDICTED: reticuline oxidase-like protein [Cucumis melo])

HSP 1 Score: 990.3 bits (2559), Expect = 6.3e-285
Identity = 486/530 (91.70%), Postives = 513/530 (96.79%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP S P+AAA S+YTTFLHCFESNS+PS GVSAIVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSTPTAAADSVYTTFLHCFESNSNPSAGVSAIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FI+LDMSNLRAVTVDVADQSAWV+AGA LGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIVLDMSNLRAVTVDVADQSAWVEAGAILGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGLSVDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLSVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RATVIALFLGKSEELVSLL KEFPELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRATVIALFLGKSEELVSLLKKEFPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNWDEPG +ADQ+FVKQIRRLYSFMTPFV+KNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWDEPGPEADQQFVKQIRRLYSFMTPFVTKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT SSK
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTLSSK 530

BLAST of Lsi01G000580 vs. NCBI nr
Match: KAA0052469.1 (reticuline oxidase-like protein [Cucumis melo var. makuwa] >TYK13353.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 485/530 (91.51%), Postives = 513/530 (96.79%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP S P+AAA S+YTTFLHCFESNS+PS GVSAIVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSTPTAAADSVYTTFLHCFESNSNPSAGVSAIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FI+LDMSNLRAVTVDVADQSAWV+AGA LGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIVLDMSNLRAVTVDVADQSAWVEAGAILGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGLSVDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLSVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RATVIALFLGKSEELVSLL K+FPELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRATVIALFLGKSEELVSLLKKKFPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNWDEPG +ADQ+FVKQIRRLYSFMTPFV+KNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWDEPGPEADQQFVKQIRRLYSFMTPFVTKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT SSK
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTLSSK 530

BLAST of Lsi01G000580 vs. NCBI nr
Match: XP_004134553.2 (berberine bridge enzyme-like 21 [Cucumis sativus])

HSP 1 Score: 974.9 bits (2519), Expect = 2.7e-280
Identity = 478/528 (90.53%), Postives = 509/528 (96.40%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP SAP+AAA S+Y+TFLHCF+SNS+ S GVS+IVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSAPTAAADSVYSTFLHCFQSNSNSSAGVSSIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FIILDMSNLR VTVDVADQSAWV AGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIILDMSNLRTVTVDVADQSAWVGAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGL+VDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLAVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RA+V+ALFLGKSEELVSLL KE PELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRASVVALFLGKSEELVSLLKKELPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGL+WLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLNWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNW+EPG +ADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWNEPGPEADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRS 529
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT S
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTHS 528

BLAST of Lsi01G000580 vs. NCBI nr
Match: KAE8647768.1 (hypothetical protein Csa_004266 [Cucumis sativus])

HSP 1 Score: 974.9 bits (2519), Expect = 2.7e-280
Identity = 478/528 (90.53%), Postives = 509/528 (96.40%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAAAASLYTTFLHCFESNSSPSDGVSAIVFDRQNASYTSVLR 60
           M P +P L LLLLP SAP+AAA S+Y+TFLHCF+SNS+ S GVS+IVF R+NASYTSVLR
Sbjct: 1   MFPLIPLLCLLLLPFSAPTAAADSVYSTFLHCFQSNSNSSAGVSSIVFARENASYTSVLR 60

Query: 61  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 120
           AYIRNARFNTSSAPKP+IIVTPL ESHVQSAVICSKK+GIQLKIRSGGHDYEGVSYIS+V
Sbjct: 61  AYIRNARFNTSSAPKPVIIVTPLTESHVQSAVICSKKLGIQLKIRSGGHDYEGVSYISDV 120

Query: 121 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180
           +FIILDMSNLR VTVDVADQSAWV AGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI
Sbjct: 121 EFIILDMSNLRTVTVDVADQSAWVGAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 180

Query: 181 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 240
           SGGGYGNMLRKYGL+VDH+LDARIVDVKGRILDS+SMGEDLFWAI+GGGGASFGVVLAYK
Sbjct: 181 SGGGYGNMLRKYGLAVDHVLDARIVDVKGRILDSKSMGEDLFWAIKGGGGASFGVVLAYK 240

Query: 241 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 300
           IRLVPVPETVT+FRVERTIEQNAADLVVRWQEVAP+TDENLFMRLLLQPVSSK+KKGTRT
Sbjct: 241 IRLVPVPETVTIFRVERTIEQNAADLVVRWQEVAPTTDENLFMRLLLQPVSSKIKKGTRT 300

Query: 301 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGTSPKALLD 360
           +RA+V+ALFLGKSEELVSLL KE PELGLQKENCTEMSWIDSV+WW NFDIGTSP+ALLD
Sbjct: 301 IRASVVALFLGKSEELVSLLKKELPELGLQKENCTEMSWIDSVLWWGNFDIGTSPEALLD 360

Query: 361 RNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATPFP 420
           RN+DS+GFL+RKSDYVQKPISRDGL+WLYKKMIEIGKTGLVFNPYGGKMSEI STATPFP
Sbjct: 361 RNVDSAGFLRRKSDYVQKPISRDGLNWLYKKMIEIGKTGLVFNPYGGKMSEISSTATPFP 420

Query: 421 HRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGTN 480
           HRAGNLYKIQYSVNW+EPG +ADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG N
Sbjct: 421 HRAGNLYKIQYSVNWNEPGPEADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIGIN 480

Query: 481 NNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRS 529
           NNDKNSFEDG+VYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT S
Sbjct: 481 NNDKNSFEDGKVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTHS 528

BLAST of Lsi01G000580 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 696.0 bits (1795), Expect = 2.3e-200
Identity = 348/536 (64.93%), Postives = 422/536 (78.73%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPL--SAPSAAAASLYTTFLHCF-ESNSSPSDGVSAIVFDRQNASYTS 60
           +S F    FL+ LP   SA   ++ S+Y +F+ CF +   SP   ++  VF R N S++S
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 67

Query: 61  VLRAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYI 120
           VLRAYIRN RFNTSS PKP IIVTP  + HV +AV CSK +   LKIRSGGHDYEG+SYI
Sbjct: 68  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 127

Query: 121 SEVQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVG 180
           S+  F ILDMSNLR V+VD+ADQSAW+ AGATLGEVYYRIWEKSKV G+PAGVCPTVGVG
Sbjct: 128 SDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVG 187

Query: 181 GHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVL 240
           GHISGGGYGNMLRK+GLSVD+++DA+IVDV G+ILD +SMGEDLFWAI GGGGASFGVVL
Sbjct: 188 GHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVL 247

Query: 241 AYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKG 300
            YK++LVPVPETVTVFRVE+ ++  A D+V +WQ V P TD NLF+R+L+QPV+   +K 
Sbjct: 248 GYKVKLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVT---RKK 307

Query: 301 TRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SP 360
            +TVRATV+ALFLG++EE+V+LLGKEFPEL L+KENC+EM+W  S +WW N    T   P
Sbjct: 308 VKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDP 367

Query: 361 KALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPST 420
           K  LDRN+D + F KRKSDYV   I RDG++ L+KKM E+GK GLVFNPYGGKM+E+   
Sbjct: 368 KVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVN 427

Query: 421 ATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDL 480
           ATPFPHR+  L+KIQYSV W E   + ++ F+ Q   LYSFMT FVSKNPR ++LNYRD+
Sbjct: 428 ATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDV 487

Query: 481 DIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSKA 532
           DIG N++  NS+E+G VYG KYFG+NF+RLVKVKTA DP+NFF NEQSIPT  SKA
Sbjct: 488 DIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTVLSKA 539

BLAST of Lsi01G000580 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 679.1 bits (1751), Expect = 2.9e-195
Identity = 334/533 (62.66%), Postives = 418/533 (78.42%), Query Frame = 0

Query: 5   LPFLFLLLLPLSAPSAA---AASLYTTFLHCF-ESNSSPSDGVSAIVFDRQNASYTSVLR 64
           +PF F  LL LS P ++   + S+Y +FL CF +   SP   ++  VF + N +++SVLR
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 65  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 124
           AYIRNARFNTSS  KP II+TP  ESHV +AV CSK +   LKIRSGGHDY+G+SYIS+ 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 125 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 184
            F ILDMSN+R V+VD+A  SAW+ AGATLGEVYYRIWEKS+V G+PAGVCPTVGVGGH+
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 185 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 244
           SGGGYGNM+RK+GLSVD++ DA+IVDV GR+LD ++MGEDLFWAI GGGG S+GVVL YK
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 245 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 304
           ++LVPVP  VTVFRVE+ ++  A D+V +WQ V P TD NLFMR+L+QPV+   +K  +T
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVT---RKKVKT 311

Query: 305 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SPKAL 364
           VRA+V+ALFLG+++E+V+LL KEFPELGL+KENC+EM+W  S +WW N    T   PK  
Sbjct: 312 VRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVF 371

Query: 365 LDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATP 424
           LDRN+D+S F KRKSDYV   I + G++ L+KKMIE+GK GLVFNPYGGKM+E+   A P
Sbjct: 372 LDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKP 431

Query: 425 FPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG 484
           FPHR   L+KIQYSVNW E  ++ ++ ++ Q + LYSFMT FVSKNPR S+ NYRD+DIG
Sbjct: 432 FPHR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIG 491

Query: 485 TNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSKA 532
            N++  NS+++G VYG KYFGENF+RLVK+KTAVDP NFF NEQSIPT  SKA
Sbjct: 492 VNDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLKSKA 540

BLAST of Lsi01G000580 vs. TAIR 10
Match: AT1G01980.1 (FAD-binding Berberine family protein )

HSP 1 Score: 676.8 bits (1745), Expect = 1.4e-194
Identity = 336/535 (62.80%), Postives = 423/535 (79.07%), Query Frame = 0

Query: 8   LFLLLLPLSAPSAAAA----SLYTTFLHCFESNS-SPSDGVSAIVFDRQNASYTSVLRAY 67
           +FL++  L + S A A    S+Y +FL CF + + +P + +  +V  + +AS+T  LRAY
Sbjct: 7   VFLIVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAY 66

Query: 68  IRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEVQF 127
           IRNARFNTS++PKPL+++    E HVQ+ V+C+K +  QLK RSGGHDY+GVSYIS   F
Sbjct: 67  IRNARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPF 126

Query: 128 IILDMSNLRAVTVDVADQ--SAWVQAGATLGEVYYRIWEKSK---VLGYPAGVCPTVGVG 187
            +LDMS LR +TVD++D   SAWV AGATLGEVYY IW+ SK     G+PAGVCPTVG G
Sbjct: 127 FVLDMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVGAG 186

Query: 188 GHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVL 247
           GHISGGGYGNM+RKYGLSVD++ DA+IVDV GRILD +SMGEDLFWAI GGGGASFGV+L
Sbjct: 187 GHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASFGVIL 246

Query: 248 AYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKG 307
           ++KI+LVPVP  VTVFRVE+T+ +NA D+V +WQ VAP T  +LFMRL+LQPV+   +  
Sbjct: 247 SFKIKLVPVPPRVTVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVT---RNT 306

Query: 308 TRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SP 367
           T+TVRA+V+ALFLGK  +L+SLL KEFPELGL+ ENCTEM+WI SV+WWAN D  T   P
Sbjct: 307 TQTVRASVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKP 366

Query: 368 KALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPST 427
           + LLDRN DS+ FLKRKSDYV+K IS+DGLD+L KK++E GK GLVFNPYGGKMSE+ +T
Sbjct: 367 EILLDRNPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATT 426

Query: 428 ATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDL 487
           ATPFPHR   L+K+Q+S+NW +PG+  +  F+++ R  YS+M PFV+KNPR ++LNYRDL
Sbjct: 427 ATPFPHRK-RLFKVQHSMNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDL 486

Query: 488 DIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           DIG N++  NS+ +  VYG KYFGENF+RLVKVKTAVDPENFF +EQSIPT  +K
Sbjct: 487 DIGINSHGPNSYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTK 537

BLAST of Lsi01G000580 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 674.9 bits (1740), Expect = 5.4e-194
Identity = 331/528 (62.69%), Postives = 415/528 (78.60%), Query Frame = 0

Query: 5   LPFLFLLLLPLSAPSAA---AASLYTTFLHCF-ESNSSPSDGVSAIVFDRQNASYTSVLR 64
           +PF F  LL LS P ++   + S+Y +FL CF +   SP   ++  VF + N +++SVLR
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 65  AYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSYISEV 124
           AYIRNARFNTSS  KP II+TP  ESHV +AV CSK +   LKIRSGGHDY+G+SYIS+ 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 125 QFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGVGGHI 184
            F ILDMSN+R V+VD+A  SAW+ AGATLGEVYYRIWEKS+V G+PAGVCPTVGVGGH+
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 185 SGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVVLAYK 244
           SGGGYGNM+RK+GLSVD++ DA+IVDV GR+LD ++MGEDLFWAI GGGG S+GVVL YK
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 245 IRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKKGTRT 304
           ++LVPVP  VTVFRVE+ ++  A D+V +WQ V P TD NLFMR+L+QPV+   +K  +T
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVT---RKKVKT 311

Query: 305 VRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--SPKAL 364
           VRA+V+ALFLG+++E+V+LL KEFPELGL+KENC+EM+W  S +WW N    T   PK  
Sbjct: 312 VRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVF 371

Query: 365 LDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPSTATP 424
           LDRN+D+S F KRKSDYV   I + G++ L+KKMIE+GK GLVFNPYGGKM+E+   A P
Sbjct: 372 LDRNLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKP 431

Query: 425 FPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRDLDIG 484
           FPHR   L+KIQYSVNW E  ++ ++ ++ Q + LYSFMT FVSKNPR S+ NYRD+DIG
Sbjct: 432 FPHR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIG 491

Query: 485 TNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPT 527
            N++  NS+++G VYG KYFGENF+RLVK+KTAVDP NFF NEQSIPT
Sbjct: 492 VNDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPT 535

BLAST of Lsi01G000580 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 670.6 bits (1729), Expect = 1.0e-192
Identity = 332/536 (61.94%), Postives = 417/536 (77.80%), Query Frame = 0

Query: 1   MSPFLPFLFLLLLPLSAPSAA---AASLYTTFLHCFESNS-SPSDGVSAIVFDRQNASYT 60
           M  F   LFL+   +S   A      ++Y  FL CF + + +P + ++ +V  +  A++T
Sbjct: 1   MKIFCLILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFT 60

Query: 61  SVLRAYIRNARFNTSSAPKPLIIVTPLIESHVQSAVICSKKVGIQLKIRSGGHDYEGVSY 120
            VLRAYIRNARFNT++ PKP I++    ESHVQ+AVIC+K + IQLK RSGGHDYEGVSY
Sbjct: 61  PVLRAYIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSY 120

Query: 121 ISEVQFIILDMSNLRAVTVDVADQSAWVQAGATLGEVYYRIWEKSKVLGYPAGVCPTVGV 180
           IS V F +LDMSNLR +TVD A +SAWV AGATLGEVYYRIWEK+K  G+PAGVCPTVG 
Sbjct: 121 ISHVPFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGA 180

Query: 181 GGHISGGGYGNMLRKYGLSVDHILDARIVDVKGRILDSESMGEDLFWAIRGGGGASFGVV 240
           GGHISGGGYGNM+RKYGLSVD++ DA+IVDV G++LD + MGED+FWAI GGGGASFGV+
Sbjct: 181 GGHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVI 240

Query: 241 LAYKIRLVPVPETVTVFRVERTIEQNAADLVVRWQEVAPSTDENLFMRLLLQPVSSKVKK 300
           LA+KI+LVPVP TVTVFRVE+ + +NA ++V +WQ VAP TD  LFMRLLLQPV+   + 
Sbjct: 241 LAFKIKLVPVPPTVTVFRVEKNLVENATEMVHKWQFVAPKTDPGLFMRLLLQPVT---RN 300

Query: 301 GTRTVRATVIALFLGKSEELVSLLGKEFPELGLQKENCTEMSWIDSVVWWANFDIGT--S 360
             +TVRA+V+ALFLG    ++S+L KEFPELGL+KENCTEM+WI SV+WWAN D  T   
Sbjct: 301 KMQTVRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIK 360

Query: 361 PKALLDRNIDSSGFLKRKSDYVQKPISRDGLDWLYKKMIEIGKTGLVFNPYGGKMSEIPS 420
           P+ LLDRN D + F KRKSD+V+K I++DGLD+L+KKMIE+GK GLVFNPYGG MS + +
Sbjct: 361 PEILLDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVAT 420

Query: 421 TATPFPHRAGNLYKIQYSVNWDEPGSKADQEFVKQIRRLYSFMTPFVSKNPRQSFLNYRD 480
           T TPFPHR   LYKIQ+S+NW +PG++A+  F+++ +  YS+M PFV+KNPR +++NYRD
Sbjct: 421 TKTPFPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRD 480

Query: 481 LDIGTNNNDKNSFEDGRVYGFKYFGENFERLVKVKTAVDPENFFWNEQSIPTRSSK 531
           LDIG N    NS+    V+G  YFGENF+RLVKVKTAVDP+NFF +EQSIPT   K
Sbjct: 481 LDIGVNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLPGK 532