Lsi01G000400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G000400
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RRM domain-containing protein
Location	chr01: 450485 .. 455321 (+)
RNA-Seq Expression	Lsi01G000400
Synteny	Lsi01G000400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATTGTTTGGGGTTATGAAAATAAGAAACCGCGAATTCGGGGTTTTGAGGTGCGAACGAACGAACGAAGCTCTACGGCGTCGACTGGGCAGATTTGAGCTCCAACATGTTGGCGGCCACCGTCTCCTTCCCTTCAATTTCAAATCTTCGGGGACCCATTCTTCTGAATAATCGATTGGACGGTTCAAACCGCCTATCTCTGAATCTTCGAGCTTCTTTCTTTGATTATCCTCTTGCCAGCAGACTCATGGTTAGAAGTAAATTCACTTGCCTCTTCCTTTCGCCTTGCTCTGCATTTTACCACCCCTTCTACTTGATTTCCTTCATTTCCCATTTGTAAATTTCCCCCCAATTTAATGTGTTTGCTGTTTCTTTCTTTTTTATTTAGCTTTTTTGGTTGCTGTCTCGTTTCTTCTTGGTTTTGCCCTATTGTTTTTTATGCTATTTTTGTGCCTTTGGTTTATTTTTCAATGCTTGTTTCTGCTGAAAGTTTATGCTGTTGTCTGCCTGATTACTCTAATCAGTGGTTTTTTTATACCAACGATGAAATTTATACAGCTAGCTAAGTTTGAAGGGTCAAAACTGAACAGTATGCGAGTTGTTTTAAGAAGAGAAGGTACCGTGAAAGTTCTGCGTGGTTCATTATAAATGACCGAGCAACTTAGATATTCAACGACATTGAATTGAAAATTTCCAAAGTTTATTAAGGATTGTTTAAGGAGTTGTAAGAAAGAAATTGGCACTAGACTAAAAAGGGTGGGGTGATAAATTATCCCCCTCCCCAAGAAAGGGAAAACTGCAAGATAGTCCTTCAATCTAAACTAATAAACCCATCGGCAAGTGAAATAGTATATGAAACTGTAAACTCCCAAGCAGTGAGATAAGACCAGGAATTTGACCAAGTGCCTGTCTAAAAAGAAAATGAACTGTATATAAACAGGTATAATTATTAAAAGAAAAATGGGACAGTTGCAAATATAGCAATCAGATTTAAAGTATTAGCATATATAGCAACATTTTTTAAAAATTGTAAATATAACAAAGTCTATCAGTTTATATACTTAGTTATTATCCAAAAAAAAACCCTGGCGACAGTGTTCAAAGAAATTTTTAGTTGAATTGATTTAGTTTGAAACTTTTCTTATCTTCATGTCTCTGTTTGAAGGTGACCAGAGATACAAACTTGGTTGTCTTGCTGATGGAAGTTATAAATCCTTCATATTCTTAGCATAGCCTATGACTCTATTTTGTCTACAGAACTTTCAGTTAGATAAATTCAGAAGCTTAAAGGATGGTTTTTCTTTTTTCATGAAGGTTACATATCAATTAGGTCAAATTTGATTCCCGAAAATGGGATTAAAAAGTATACCATAATAATGTTGACACCTTAGATGTCCACTAGCATATTGATAATGATGTTTAATGCAAGTGATGTGGAGTGGATAATAAAGCATTTATGGAATACTCGAACTCATGTATTGATAATTATGTGGACTTTTGAGTTTCGACACAAATCTTTTCTTCTTTTCAATGTACTCCTTTTACTTTTAAGAATATGGCTTGAAAGGATTAATAATTTATTTGAAGTGAAAAAAAAAAAAAATTAAGGTGGACTGTGGCCCTTGGTGTATATCTATGAAAATTTTCCAGCTCTTCACATTAGCTTTTTCTTGGAGTTCCAAATCAACTTCCTTTCCTCTATTTTATTGGTATGTGTTTGTCCTGCAATATGGTGACTTTCACCTTCTTTAATGTCAATTATCCAGCCATCCTCACATTTGGAGTGTACTTATCCATATCGAGGTTTTTATGGAAAACTATTGGTGACGTTCTTCACTTATTTTAACTGATTCAGGAAAAGTGGGAAGAGAAAAAACAAAGCAGCATTACTGTCTTTTCTATTTGTTTAGCTGTTTTTATTTTTCAGTTTTTCCTGTTTTCATTATACTGATAATTTATTATGATTACTGAGTTCTGTTTATGCATGGTGTAACTTAATACTAGTGAAAGTAAAAAGTCAACAGAAAACAAAGACAATAAGAAAATAACCTTTCCAAACTGTCTTTTGAAGTAAAATTATTGAAAATATTTTTCTTATTATTGTTGGAAAGCAGATTTTCAATAATTTTTTAGAGAAGTTTTAGATGGCATAGTTCACTTCTAATACATATGCCTTCTGAAAACAACTTTTTCCTCATAAGTTTTCTTCTGCTATTTTCTTCTTCTCGTATTTTTCTTGATGTTTTGTGAAGTCTTTTGGTATCTCATTGTCTAGATTTTAAGAGGCCTGTATCGGAGGTTATTGCAATATCAAATCCGTGGAATCCTTTTTCTTGAGCACCTATGAATGTCCAAATGCTGCTTCCATCGAGTTGTTTTTTTTAAAAAAAATAAATAAATAAAATTATGCAGATTTACCATACTCCACCAATGAGAGTCGGCTGCAAGAAGAATTTTCAAATTTTGGTGAGATTGCTGAAGGTAATTGCATTTCTTATTGGCTAATCTCCAGCCAAGTCAAATAAGCCTTTTAATCTTGTTGCTTCACTTATCAACCCATGGCAGTGTTATTAGCAAAAGATCGGCTAACAAAAAGACCAAAAGGTTATGCATTTATTCAATACACTTGCCAAGACGATGCCATGCTTGCACTGGAAAATATGGATTGCAAGGTTAAACTTGACAAACTTCACCTAGTTCTATATTTTGTAGATAAGATAACTCATGCTTGAAAGTTAATGTACTTTCTGTGTCTCTCAAAACCAGAAATTTGATGGAAGGATGATCTATGTGGAACTTGCAAAACCTGGTAGTGATTCCTTTGGAGGATATCCGAAAACCTCTGGACCCCCAAAAGAGCGGCCTAAAGTGGAGGTAGAAGAGGTGGCAGATTGCTGGTATTGATCATCTTCTTACTGAGATACACAATATATAGGACAGAGATGGCTTAACAGTTAATAATGACAGTTGTGCCCAATGAGATTCAGATCAATCCCAAATCATTTGTGAGTGCTGAAGTGTTTGCTAAGATTACATTTATTTTCATAGTTTCAAGCTTTAGATGCTGATGCCATTCTGCAACACTTCTCCTGTAGTTTGTTACTGTAACAGATATCTATTTGTAATTACACTGTTTATGACACTTAATGCATCTTCTAGTGCATATTGCTTTTTGTAGCTTTGCAATTGAAGATTATGGAAAATGCGGAAATTCGTAATGCAATTTTTTTTTCTTTTTCCCATCCAATAATCTGTTTCCAAATTTAAAGTGTATTATGTTTTTTCAATCGGTTCTTATTTCTTGATTTGCACTCAACTTTTGGTATGATAATGCAAGAAGCGGCCCAGGGACACCTACACAGAGATTGCGTATTTGAATTTCTGAGGGAGCTTAATAAGAAAAATTGTTGATATCTCTAAATCTTTTTCTTGATAAAAACAAACGTAGGTGTTTATGAGATATATTTATATAATTTTTACAATCAAAGAATTAATGAGTTTCATTTCTTGCATTATACTCATGATCTACTTTAGCTTTCAAATGTTTGGTAGAAGTCAAGGCTTAAATTAATGTGGGTGACAAATTTCTCCTATATCATCCACTTCTAGACTGGGGTCATAGTCCAACCACACATAGTGCTTTGTTCTATCTTCTCAAAAAGGAGACAATTCTAAAGTAGCTTTTATTTAACTTTTGAAGCTTGATCAATGGATAGTTCTATACAGTTACCCATTTCTTCAACTCAAAAGAAGATAACAAAAGAATAGGCAACACCAAAATCTAGTTTTATTGCTTACTGACTTCAACTAATTTCATCTATCAATCTCAACTCTTGAAAATTGGTAGCATCTTCTAAAGAAGTCAATCCATGTGATCAATTTTATTGCTTACCTGATTCACTTTTAAGTCTACTGCTTCTTGATTTGTGCAATTTTACTTGACATTTACACAATGTGTTCTATAGAATCCTTGGACTTCAGCTGATGCCTGCAAGTGGGGCAAGAAGAGTGAGACAGCAGCCATTGATCAATGCAAGTGACATGGTACCTGTGAGCGCAGTTCGGCAACACCCGTATTTTATCCCCGTTAGAGAAATCAACGAGGCAGATAGCACAGGCCGGATTCGATGAAGGCGAAGTCAGTGAGCCAGAATTCGTGTAAGTTGAAGTTGGCAAGGCAACCACGTCTTGCTTCTTCATGCCTGAGTTCAGTCCCCTTAAAGCCACCCATTGGACTGTTTGTCTGAGGGTGTGATTGGCGCATTGAAGTATACATATCAGCATGCTATTTAGACCAAGTGCACAAACAACAGCGCAAACAATCGCAACCACGATGACAATGAGGTTGAAGTTGCCACCTTGTTCTTGGTCGCAAGCATCCGGGATAGATACGCTTGGCGATGGATTAAGGGAAGGAGAAGACGAAGGCTCCATGAACGAAGAACCGAGCATTTTTCGCAAGATGAAATGGTTGAGAAGGAAGCAGCATATAAACATAAATATGAAACAAGTTGAGATAAAGAAACATAAGTTTTGAAGGAGGGCATATGATAAAATTAGATATTTGTGGACAGAGAGCTAGGTGGGTATGGGTTGAATTCAACATGGAAGGGTGAGAATGTGATGGGAAGTAGTGGGGTTATTCGAAAAATGAATCAACCTCTTTTTTCTTTTGTTTGTGCGCTACAAACAGAAAAGGCAGTCTCATTGTCCCCTCACTTTCTTTCATCCTTAGCACCAAAGAAAAACAGTCAATTCCCCCATGAGGCTATTTTCGGTAGGGACAACAGAACCTGATATATATAGAGTTAAAAATAACACATTTCAAGAGCCAG

mRNA sequence

AAAAATTGTTTGGGGTTATGAAAATAAGAAACCGCGAATTCGGGGTTTTGAGGTGCGAACGAACGAACGAAGCTCTACGGCGTCGACTGGGCAGATTTGAGCTCCAACATGTTGGCGGCCACCGTCTCCTTCCCTTCAATTTCAAATCTTCGGGGACCCATTCTTCTGAATAATCGATTGGACGGTTCAAACCGCCTATCTCTGAATCTTCGAGCTTCTTTCTTTGATTATCCTCTTGCCAGCAGACTCATGGTTAGAATGTTATTAGCAAAAGATCGGCTAACAAAAAGACCAAAAGGTTATGCATTTATTCAATACACTTGCCAAGACGATGCCATGCTTGCACTGGAAAATATGGATTGCAAGAAATTTGATGGAAGGATGATCTATGTGGAACTTGCAAAACCTGGTAGTGATTCCTTTGGAGGATATCCGAAAACCTCTGGACCCCCAAAAGAGCGGCCTAAAGTGGAGGTAGAAGAGGTGGCAGATTGCTGGTATTGATCATCTTCTTACTGAGATACACAATATATAGGACAGAGATGGCTTAACAGTTAATAATGACAGTTGTGCCCAATGAGATTCAGATCAATCCCAAATCATTTAATCCTTGGACTTCAGCTGATGCCTGCAAGTGGGGCAAGAAGAGTGAGACAGCAGCCATTGATCAATGCAAGTGACATGGTACCTGTGAGCGCAGTTCGGCAACACCCGTATTTTATCCCCGTTAGAGAAATCAACGAGGCAGATAGCACAGGCCGGATTCGATGAAGGCGAAGTCAGTGAGCCAGAATTCGTGTAAGTTGAAGTTGGCAAGGCAACCACGTCTTGCTTCTTCATGCCTGAGTTCAGTCCCCTTAAAGCCACCCATTGGACTGTTTGTCTGAGGGTGTGATTGGCGCATTGAAGTATACATATCAGCATGCTATTTAGACCAAGTGCACAAACAACAGCGCAAACAATCGCAACCACGATGACAATGAGGTTGAAGTTGCCACCTTGTTCTTGGTCGCAAGCATCCGGGATAGATACGCTTGGCGATGGATTAAGGGAAGGAGAAGACGAAGGCTCCATGAACGAAGAACCGAGCATTTTTCGCAAGATGAAATGGTTGAGAAGGAAGCAGCATATAAACATAAATATGAAACAAGTTGAGATAAAGAAACATAAGTTTTGAAGGAGGGCATATGATAAAATTAGATATTTGTGGACAGAGAGCTAGGTGGGTATGGGTTGAATTCAACATGGAAGGGTGAGAATGTGATGGGAAGTAGTGGGGTTATTCGAAAAATGAATCAACCTCTTTTTTCTTTTGTTTGTGCGCTACAAACAGAAAAGGCAGTCTCATTGTCCCCTCACTTTCTTTCATCCTTAGCACCAAAGAAAAACAGTCAATTCCCCCATGAGGCTATTTTCGGTAGGGACAACAGAACCTGATATATATAGAGTTAAAAATAACACATTTCAAGAGCCAG

Coding sequence (CDS)

ATGTTGGCGGCCACCGTCTCCTTCCCTTCAATTTCAAATCTTCGGGGACCCATTCTTCTGAATAATCGATTGGACGGTTCAAACCGCCTATCTCTGAATCTTCGAGCTTCTTTCTTTGATTATCCTCTTGCCAGCAGACTCATGGTTAGAATGTTATTAGCAAAAGATCGGCTAACAAAAAGACCAAAAGGTTATGCATTTATTCAATACACTTGCCAAGACGATGCCATGCTTGCACTGGAAAATATGGATTGCAAGAAATTTGATGGAAGGATGATCTATGTGGAACTTGCAAAACCTGGTAGTGATTCCTTTGGAGGATATCCGAAAACCTCTGGACCCCCAAAAGAGCGGCCTAAAGTGGAGGTAGAAGAGGTGGCAGATTGCTGGTATTGA

Protein sequence

MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSDSFGGYPKTSGPPKERPKVEVEEVADCWY

Homology

[Close]

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot
Match: Q9FX45 (Organelle RRM domain-containing protein 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ORRM6 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 28/74 (37.84%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 48  MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSDSFGG 107
           ++ M +  D++  RPKG+AF++Y  +++AM A++ M  K  DGR+I+VE AK  SD    
Sbjct: 103 LIHMNMVMDKVANRPKGFAFLRYETEEEAMKAIQGMHGKFLDGRVIFVEEAKTRSDMSRA 162

Query: 108 YPKTSGP-PKERPK 121
            P+   P P+ +P+
Sbjct: 163 KPRRDFPKPQSKPR 176

[Close]

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot
Match: Q6C747 (Eukaryotic translation initiation factor 3 subunit G OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=TIF35 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-05
Identity = 28/67 (41.79%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 33  NLRASFFDYPLASRLMVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRM 92
           +LR  F  Y + +R+ V    AKDR T RP+G+AF+ YT +  A  ALE MD   FD  +
Sbjct: 229 DLRRMFGKYGMINRVYV----AKDRDTGRPRGFAFVTYTLKSHAQAALEAMDGHGFDNLI 288

Query: 93  IYVELAK 100
           + V+ +K
Sbjct: 289 MKVDYSK 291

[Close]

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot
Match: Q9FMG4 (Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC35 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.0e-05
Identity = 31/94 (32.98%), Postives = 52/94 (55.32%), Query Frame = 0


Query: 30  LSLNLRASFFD-YPLASRL--MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCK 89
           L++  R +  D YPL ++   +V + + +DR T   +G+AF++Y  +D+A  A+E +D +
Sbjct: 21  LNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKDEAHKAVERLDGR 80

Query: 90  KFDGRMIYVELAK--PGSDSFGGYPKTSGPPKER 119
             DGR I V+ AK  P ++          PPK R
Sbjct: 81  VVDGREITVQFAKYGPNAEKISKGRVVEPPPKSR 114

[Close]

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot
Match: Q8L440 (Organelle RRM domain-containing protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ORRM1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.9e-05
Identity = 22/52 (42.31%), Postives = 37/52 (71.15%), Query Frame = 0


Query: 48  MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAK 100
           +V + +  D+++KR KGYAF++YT ++ A  AL+ M+ K  +G MI V++AK
Sbjct: 308 LVEVKIIMDKISKRSKGYAFLEYTTEEAAGTALKEMNGKIINGWMIVVDVAK 359

[Close]

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot
Match: Q9FFZ6 (Small RNA-binding protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S-RBP11 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.1e-05
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 48  MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSD--SF 107
           +V   +  DR++ R KG+ F+ +   D+A  AL   + ++ +GR I+V+ AK        
Sbjct: 60  VVEAQIVMDRVSDRSKGFGFVTFASADEAQKALMEFNGQQLNGRTIFVDYAKAKQSLGGG 119

Query: 108 GGYPKTSGPP 116
           GGYP   GPP
Sbjct: 120 GGYPIARGPP 129

[Close]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL
Match: A0A1S3AYG0 (glycine-rich RNA-binding protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103484270 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.6e-52
Identity = 110/154 (71.43%), Postives = 116/154 (75.32%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M A TVS PSISNLRGP+  NN L  SNR SLNLRASFFDYPLASRLMVR          
Sbjct: 1   MWATTVSLPSISNLRGPVFPNNPLVASNRPSLNLRASFFDYPLASRLMVRNLPYSTNESR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDR TKRPKGYAFIQYTCQDDAMLALENMDCK FDGR IYVE+
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRSTKRPKGYAFIQYTCQDDAMLALENMDCKIFDGRTIYVEI 120

Query: 121 AKPGSDSFGGYPKTSGPPKERPKVEVEEVADCWY 132
           AKPGS SFGGYP+TSGPPKERP VE E++ADCWY
Sbjct: 121 AKPGSGSFGGYPRTSGPPKERPNVEQEDIADCWY 154

[Close]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL
Match: A0A6J1IJY1 (small RNA-binding protein 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111478117 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 4.4e-52
Identity = 115/156 (73.72%), Postives = 118/156 (75.64%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPS+SNLRGPI LNNRLD S R SLNLRASF DYPLASRLMVR          
Sbjct: 1   MWAATVSFPSLSNLRGPIPLNNRLDSSKRQSLNLRASFSDYPLASRLMVRNLPYSTNENR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDRLTK PKGYAFIQYT QDDAMLALENMDCKKFDGR IYVEL
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRLTKTPKGYAFIQYTSQDDAMLALENMDCKKFDGRTIYVEL 120

Query: 121 AKPGSDSFGGYPKTSGPPKER--PKVEVEEVADCWY 132
           AKPGS SFGGYP TSGPPKER  PK+  EEVADCWY
Sbjct: 121 AKPGSGSFGGYPVTSGPPKERPIPKLVPEEVADCWY 156

[Close]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL
Match: A0A0A0KL21 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526430 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.7e-51
Identity = 110/158 (69.62%), Postives = 117/158 (74.05%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M A TVSFPS+SNLRGPI  NNRL  SNR+SLNLRASFFDYPLASRLMVR          
Sbjct: 1   MWATTVSFPSVSNLRGPIFPNNRLVASNRISLNLRASFFDYPLASRLMVRNLPYSTNESR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDR TKRPKGYAFIQYTCQDDAMLALE MDCK FDGRMIYVE+
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRSTKRPKGYAFIQYTCQDDAMLALETMDCKIFDGRMIYVEI 120

Query: 121 AKPGSDSFGGYPKTSGPPKERP----KVEVEEVADCWY 132
           A PGS SFGGYP+ SGPPKERP     V+ E+VADCWY
Sbjct: 121 ATPGSGSFGGYPRASGPPKERPNERANVDQEDVADCWY 158

[Close]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL
Match: A0A6J1EGU7 (small RNA-binding protein 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111433254 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.4e-50
Identity = 113/156 (72.44%), Postives = 117/156 (75.00%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPS+SNLRGPI L+NRLD S R SLNLRASF DYPLASRLMVR          
Sbjct: 1   MWAATVSFPSLSNLRGPIPLSNRLDSSKRQSLNLRASFSDYPLASRLMVRNLPYSTNENR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDRLTK PKGYAFIQYT QDDAMLALENMDCKKFDGR IYVEL
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRLTKTPKGYAFIQYTSQDDAMLALENMDCKKFDGRTIYVEL 120

Query: 121 AKPGSDSFGGYPKTSGPPKER--PKVEVEEVADCWY 132
           AKPGS SF GYP TSGPPKER  PK+  EEVADCWY
Sbjct: 121 AKPGSGSFRGYPVTSGPPKERPIPKLVPEEVADCWY 156

[Close]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL
Match: A0A6J1CXC0 (glycine-rich RNA-binding protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111015686 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.4e-50
Identity = 106/154 (68.83%), Postives = 119/154 (77.27%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M A+TVSFP + N R PILL+N   G+ R SLNLRASFFDYPLASR+MVR          
Sbjct: 1   MWASTVSFPCLLNPRFPILLSNPTHGTARQSLNLRASFFDYPLASRVMVRNLPYSTDEGR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDR+TKRP+GYAFIQYTCQDDAMLALENMD KKFDGR IYVE+
Sbjct: 61  LQEEFSSFGEVAEVLLAKDRVTKRPRGYAFIQYTCQDDAMLALENMDGKKFDGRTIYVEV 120

Query: 121 AKPGSDSFGGYPKTSGPPKERPKVEVEEVADCWY 132
           A+PGSDSFGGYP+TSGPPKERPK+E+EEVADCWY
Sbjct: 121 ARPGSDSFGGYPRTSGPPKERPKLELEEVADCWY 154

[Close]

BLAST of Lsi01G000400 vs. NCBI nr
Match: XP_038883016.1 (organelle RRM domain-containing protein 6, chloroplastic isoform X3 [Benincasa hispida])

HSP 1 Score: 221.9 bits (564), Expect = 3.3e-54
Identity = 117/174 (67.24%), Postives = 123/174 (70.69%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPSISNLRGPIL NNR DGS R SLN RASF DYPLASRL+VR          
Sbjct: 1   MWAATVSFPSISNLRGPILPNNRTDGSKRPSLNFRASFSDYPLASRLLVRTSLNFEGSNL 60

Query: 61  ---------------------------------MLLAKDRLTKRPKGYAFIQYTCQDDAM 120
                                            +LLAKD+LTKRPKGYAFIQYTCQDDAM
Sbjct: 61  NSMRVVLRREDLPYSTKESRLQEEFSNFGEIAEVLLAKDQLTKRPKGYAFIQYTCQDDAM 120

Query: 121 LALENMDCKKFDGRMIYVELAKPGSDSFGGYPKTSGPPKERPKVEVEEVADCWY 132
           LALENMDCKKFDGRMIYVELAKPGS SFGGYP+TSGPPKERPKVE++EVADCWY
Sbjct: 121 LALENMDCKKFDGRMIYVELAKPGSSSFGGYPRTSGPPKERPKVELQEVADCWY 174

[Close]

BLAST of Lsi01G000400 vs. NCBI nr
Match: XP_038883015.1 (glycine-rich RNA-binding protein GRP2A isoform X2 [Benincasa hispida])

HSP 1 Score: 215.7 bits (548), Expect = 2.4e-52
Identity = 117/190 (61.58%), Postives = 123/190 (64.74%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPSISNLRGPIL NNR DGS R SLN RASF DYPLASRL+VR          
Sbjct: 1   MWAATVSFPSISNLRGPILPNNRTDGSKRPSLNFRASFSDYPLASRLLVRSKFTFLFLSF 60

Query: 61  -------------------------------------------------MLLAKDRLTKR 120
                                                            +LLAKD+LTKR
Sbjct: 61  QLIFHLEISPPICCVHCDVNFSVAPLDLPYSTKESRLQEEFSNFGEIAEVLLAKDQLTKR 120

Query: 121 PKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSDSFGGYPKTSGPPKERPKV 132
           PKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGS SFGGYP+TSGPPKERPKV
Sbjct: 121 PKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSSSFGGYPRTSGPPKERPKV 180

[Close]

BLAST of Lsi01G000400 vs. NCBI nr
Match: XP_008439484.1 (PREDICTED: glycine-rich RNA-binding protein 4, mitochondrial [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 5.3e-52
Identity = 110/154 (71.43%), Postives = 116/154 (75.32%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M A TVS PSISNLRGP+  NN L  SNR SLNLRASFFDYPLASRLMVR          
Sbjct: 1   MWATTVSLPSISNLRGPVFPNNPLVASNRPSLNLRASFFDYPLASRLMVRNLPYSTNESR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDR TKRPKGYAFIQYTCQDDAMLALENMDCK FDGR IYVE+
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRSTKRPKGYAFIQYTCQDDAMLALENMDCKIFDGRTIYVEI 120

Query: 121 AKPGSDSFGGYPKTSGPPKERPKVEVEEVADCWY 132
           AKPGS SFGGYP+TSGPPKERP VE E++ADCWY
Sbjct: 121 AKPGSGSFGGYPRTSGPPKERPNVEQEDIADCWY 154

[Close]

BLAST of Lsi01G000400 vs. NCBI nr
Match: XP_022977992.1 (small RNA-binding protein 11, chloroplastic [Cucurbita maxima])

HSP 1 Score: 213.8 bits (543), Expect = 9.1e-52
Identity = 115/156 (73.72%), Postives = 118/156 (75.64%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPS+SNLRGPI LNNRLD S R SLNLRASF DYPLASRLMVR          
Sbjct: 1   MWAATVSFPSLSNLRGPIPLNNRLDSSKRQSLNLRASFSDYPLASRLMVRNLPYSTNENR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDRLTK PKGYAFIQYT QDDAMLALENMDCKKFDGR IYVEL
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRLTKTPKGYAFIQYTSQDDAMLALENMDCKKFDGRTIYVEL 120

Query: 121 AKPGSDSFGGYPKTSGPPKER--PKVEVEEVADCWY 132
           AKPGS SFGGYP TSGPPKER  PK+  EEVADCWY
Sbjct: 121 AKPGSGSFGGYPVTSGPPKERPIPKLVPEEVADCWY 156

[Close]

BLAST of Lsi01G000400 vs. NCBI nr
Match: XP_023543104.1 (small RNA-binding protein 11, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210.7 bits (535), Expect = 7.7e-51
Identity = 114/156 (73.08%), Postives = 117/156 (75.00%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSLNLRASFFDYPLASRLMVR---------- 60
           M AATVSFPS+SNLRGPI LNNRLD S R SLNLRASF DYPLASRLMVR          
Sbjct: 1   MWAATVSFPSLSNLRGPIPLNNRLDSSKRQSLNLRASFSDYPLASRLMVRNLPYSTNENR 60

Query: 61  -------------MLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVEL 120
                        +LLAKDRLTK PKGYAFIQYT QDDAMLALENMDCKKFDGR IYVEL
Sbjct: 61  LQEEFSNFGEIAEVLLAKDRLTKTPKGYAFIQYTSQDDAMLALENMDCKKFDGRTIYVEL 120

Query: 121 AKPGSDSFGGYPKTSGPPKER--PKVEVEEVADCWY 132
           AKPGS SF GYP TSGPPKER  PK+  EEVADCWY
Sbjct: 121 AKPGSGSFRGYPVTSGPPKERPIPKLVPEEVADCWY 156

[Close]

BLAST of Lsi01G000400 vs. TAIR 10
Match: AT4G20030.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 117.9 bits (294), Expect = 6.3e-27
Identity = 68/158 (43.04%), Postives = 91/158 (57.59%), Query Frame = 0


Query: 1   MLAATVSFPSISNLRGPILLNNRLDGSNRLSL-NLRASFFDYPLASRLMVRML------- 60
           M +AT+SFPS       +  ++ L     L    ++AS F+YPLAS++MVR L       
Sbjct: 1   MWSATLSFPSF------VASSSSLPNYRNLRFPKIKASLFNYPLASKIMVRNLPFSTSED 60

Query: 61  ----------------LAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVE 120
                           L KD   KR KGYAFIQ+T QDDA LA+E MD + ++GRMIY++
Sbjct: 61  FLKREFSAFGEIAEVKLIKDEAMKRSKGYAFIQFTSQDDAFLAIETMDRRMYNGRMIYID 120

Query: 121 LAKPGSDSFGGYPKTSGPPKERPKVEV---EEVADCWY 132
           +AKPG   F G P+TSGPP++  + E    +EVADCWY
Sbjct: 121 IAKPGKRDFQGLPRTSGPPEKSDEPEEAANDEVADCWY 152

[Close]

BLAST of Lsi01G000400 vs. TAIR 10
Match: AT1G73530.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.2e-09
Identity = 28/74 (37.84%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 48  MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSDSFGG 107
           ++ M +  D++  RPKG+AF++Y  +++AM A++ M  K  DGR+I+VE AK  SD    
Sbjct: 103 LIHMNMVMDKVANRPKGFAFLRYETEEEAMKAIQGMHGKFLDGRVIFVEEAKTRSDMSRA 162

Query: 108 YPKTSGP-PKERPK 121
            P+   P P+ +P+
Sbjct: 163 KPRRDFPKPQSKPR 176

[Close]

BLAST of Lsi01G000400 vs. TAIR 10
Match: AT3G46020.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 55.1 bits (131), Expect = 5.0e-08
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 53  LAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCKKFDGRMIYVELAKPGSDSFGG 108
           L +D  T+RPKG+ FI +  +DDA  AL+++D K  DGR+I+VE+AK   +   G
Sbjct: 38  LIRDSETQRPKGFGFITFDSEDDARKALKSLDGKIVDGRLIFVEVAKNAEEVRAG 92

[Close]

BLAST of Lsi01G000400 vs. TAIR 10
Match: AT5G59860.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-06
Identity = 33/89 (37.08%), Postives = 45/89 (50.56%), Query Frame = 0


Query: 15  RGPILLNNRLDGSNRLSL----NLRASFFDYPLASRLMVRMLLAKDRLTKRPKGYAFIQY 74
           R  I    RL G   L+      L A   D  L         L KD+ T+RPKG+ FI +
Sbjct: 55  RSSISKRRRLIGGKTLTCVSNSGLSAYTTDQSLRQLFAPFARLIKDQQTQRPKGFGFITF 114

Query: 75  TCQDDAMLALENMDCKKFDGRMIYVELAK 100
             +DDA  AL+ ++ K  +GR+I+VE AK
Sbjct: 115 ESEDDAQKALKALNGKIVNGRLIFVETAK 143

[Close]

BLAST of Lsi01G000400 vs. TAIR 10
Match: AT5G64200.1 (ortholog of human splicing factor SC35 )

HSP 1 Score: 49.7 bits (117), Expect = 2.1e-06
Identity = 31/94 (32.98%), Postives = 52/94 (55.32%), Query Frame = 0


Query: 30  LSLNLRASFFD-YPLASRL--MVRMLLAKDRLTKRPKGYAFIQYTCQDDAMLALENMDCK 89
           L++  R +  D YPL ++   +V + + +DR T   +G+AF++Y  +D+A  A+E +D +
Sbjct: 21  LNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKDEAHKAVERLDGR 80

Query: 90  KFDGRMIYVELAK--PGSDSFGGYPKTSGPPKER 119
             DGR I V+ AK  P ++          PPK R
Sbjct: 81  VVDGREITVQFAKYGPNAEKISKGRVVEPPPKSR 114

The following BLAST results are available for this feature:

BLAST of Lsi01G000400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FX45	1.7e-08	37.84	Organelle RRM domain-containing protein 6, chloroplastic OS=Arabidopsis thaliana...	[more]
Q6C747	1.3e-05	41.79	Eukaryotic translation initiation factor 3 subunit G OS=Yarrowia lipolytica (str...	[more]
Q9FMG4	3.0e-05	32.98	Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC3...	[more]
Q8L440	3.9e-05	42.31	Organelle RRM domain-containing protein 1, chloroplastic OS=Arabidopsis thaliana...	[more]
Q9FFZ6	5.1e-05	34.29	Small RNA-binding protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S...	[more]

BLAST of Lsi01G000400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AYG0	2.6e-52	71.43	glycine-rich RNA-binding protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1IJY1	4.4e-52	73.72	small RNA-binding protein 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0KL21	3.7e-51	69.62	RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526430 PE=4 SV...	[more]
A0A6J1EGU7	1.4e-50	72.44	small RNA-binding protein 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1CXC0	2.4e-50	68.83	glycine-rich RNA-binding protein 4, mitochondrial OS=Momordica charantia OX=3673...	[more]

BLAST of Lsi01G000400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883016.1	3.3e-54	67.24	organelle RRM domain-containing protein 6, chloroplastic isoform X3 [Benincasa h...	[more]
XP_038883015.1	2.4e-52	61.58	glycine-rich RNA-binding protein GRP2A isoform X2 [Benincasa hispida]	[more]
XP_008439484.1	5.3e-52	71.43	PREDICTED: glycine-rich RNA-binding protein 4, mitochondrial [Cucumis melo]	[more]
XP_022977992.1	9.1e-52	73.72	small RNA-binding protein 11, chloroplastic [Cucurbita maxima]	[more]
XP_023543104.1	7.7e-51	73.08	small RNA-binding protein 11, chloroplastic [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi01G000400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20030.1	6.3e-27	43.04	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G73530.1	1.2e-09	37.84	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT3G46020.1	5.0e-08	45.45	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G59860.1	1.2e-06	37.08	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G64200.1	2.1e-06	32.98	ortholog of human splicing factor SC35	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 44..121 e-value: 4.0E-13 score: 51.5
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 52..93 e-value: 2.5E-7 score: 30.4
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 17..100 score: 10.309022
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 102..121
None	No IPR available	PANTHER	PTHR13952:SF19	GLYCINE-RICH RNA-BINDING PROTEIN 4, MITOCHONDRIAL ISOFORM X1	coord: 53..126
None	No IPR available	PANTHER	PTHR13952	U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD	coord: 53..126
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 50..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G000400.1	Lsi01G000400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0016607	nuclear speck
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding