Lsi01G000070 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi01G000070 vs. ExPASy Swiss-Prot
Match: P29503 (Protein neuralized OS=Drosophila melanogaster OX=7227 GN=neur PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 6.0e-11
Identity = 34/57 (59.65%), Postives = 41/57 (71.93%), Query Frame = 0

Query: 772 KCCICYNMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCRAPIEDVVRASFTT 826
           +C ICY   IDS+LY CGHMC C  CA E QWR   GG+CP+CRA I DV+R ++TT
Sbjct: 700 ECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR-TYTT 754

BLAST of Lsi01G000070 vs. ExPASy Swiss-Prot
Match: Q24746 (Protein neuralized OS=Drosophila virilis OX=7244 GN=neur PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.0e-11
Identity = 34/57 (59.65%), Postives = 41/57 (71.93%), Query Frame = 0

Query: 772 KCCICYNMQIDSLLYRCGHMCSCMKCAKELQWR---GGKCPVCRAPIEDVVRASFTT 826
           +C ICY   IDS+LY CGHMC C  CA E QWR   GG+CP+CRA I DV+R ++TT
Sbjct: 693 ECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR-TYTT 747

BLAST of Lsi01G000070 vs. ExPASy Swiss-Prot
Match: Q0MW30 (E3 ubiquitin-protein ligase NEURL1B OS=Mus musculus OX=10090 GN=Neurl1b PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-07
Identity = 22/54 (40.74%), Postives = 37/54 (68.52%), Query Frame = 0

Query: 768 SRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRG-GKCPVCRAPIEDVVR 821
           SR  +C +C++ ++D+++Y CGHMC C  C   L+ +    CP+CR PI+DV++
Sbjct: 489 SRNGECTVCFDSEVDTVIYTCGHMCLCHGCGLRLRRQARACCPICRRPIKDVIK 542

BLAST of Lsi01G000070 vs. ExPASy Swiss-Prot
Match: Q923S6 (E3 ubiquitin-protein ligase NEURL1 OS=Mus musculus OX=10090 GN=Neurl1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.0e-07
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 756 SIKRNEVGGGRKSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWR-GGKCPVCRAP 815
           S+  + V  G      +C ICY   +D+++Y CGHMC C  C   L+      CP+CR P
Sbjct: 504 SLPESPVTPGLGQWSDECTICYEHAVDTVIYTCGHMCLCYSCGLRLKKALHACCPICRRP 563

Query: 816 IEDVVR 821
           I+D+++
Sbjct: 564 IKDIIK 569

BLAST of Lsi01G000070 vs. ExPASy Swiss-Prot
Match: O76050 (E3 ubiquitin-protein ligase NEURL1 OS=Homo sapiens OX=9606 GN=NEURL1 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.3e-07
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 0

Query: 756 SIKRNEVGGGRKSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWR-GGKCPVCRAP 815
           S+  + V  G      +C ICY   +D+++Y CGHMC C  C   L+      CP+CR P
Sbjct: 504 SLPESPVTPGLGQWSDECTICYEHAVDTVIYTCGHMCLCYACGLRLKKALHACCPICRRP 563

Query: 816 IEDVVR 821
           I+D+++
Sbjct: 564 IKDIIK 569

BLAST of Lsi01G000070 vs. ExPASy TrEMBL
Match: A0A1S3AYB9 (uncharacterized protein LOC103484234 OS=Cucumis melo OX=3656 GN=LOC103484234 PE=4 SV=1)

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 693/842 (82.30%), Postives = 734/842 (87.17%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             NQN NP+L NF V KTNH+T+T    +RSDDS+QH S TNDPQTQT STP P   PIP
Sbjct: 61  --NQNTNPVLTNFPVTKTNHDTST----KRSDDSQQHAS-TNDPQTQTTSTPAP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQ 180
           ET  NKNQTSKLGASSLVQIWEKRLNVSSSN+GLNANANA N  VCS KQETE     EQ
Sbjct: 121 ETSPNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANA-NTAVCSTKQETEA----EQ 180

Query: 181 EQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240
           EQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV
Sbjct: 181 EQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240

Query: 241 VDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSPRIRGR 300
           VDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIP+DQVEP+CLSHILCSPRIRGR
Sbjct: 241 VDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGR 300

Query: 301 QAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360
           QAFADLLLQIERDRQRELD+LV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF
Sbjct: 301 QAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360

Query: 361 VITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNA--HTPDPHAT 420
           VITPRENHRS +IMHLRERFSGVGEN A+S  GEMLN+ND+DK Q  TNA  HTP PH  
Sbjct: 361 VITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHTP-PHVI 420

Query: 421 NVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPEREQEQE 480
           N TN+K+K    DNDNQQ VVG+HS + D N+QI+G FKEEQIE+QE  KP+ E E EQ 
Sbjct: 421 N-TNEKDK----DNDNQQ-VVGIHSNNTD-NDQIIGGFKEEQIEKQE-QKPDQELEPEQ- 480

Query: 481 EEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDWFS 540
              G EVDPPS EGIWQD   LN DS DS++GWEAE Q SEA EESYGADYVGTSYDWF+
Sbjct: 481 ---GGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQ-SEAGEESYGADYVGTSYDWFA 540

Query: 541 DISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTRLE 600
           DISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLMVTRLE
Sbjct: 541 DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLE 600

Query: 601 RQTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE-DDERSLISAQYHEASDY 660
           RQTQQEEEY    EDDD   +EE+WCFSEGHTQPKSSDNEEE DDERSLI+AQYHEASDY
Sbjct: 601 RQTQQEEEYNEVKEDDD--VDEELWCFSEGHTQPKSSDNEEEDDDERSLITAQYHEASDY 660

Query: 661 LDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQRSSLRS 720
           LDQSASPLQLASPSILSSWSY    +MGEDSNRG S SSPQP  PQFSS NNQ R SL S
Sbjct: 661 LDQSASPLQLASPSILSSWSY----DMGEDSNRGTSISSPQPLLPQFSSNNNQHRPSLVS 720

Query: 721 TTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNEVGGGR---- 780
            +H+PSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR+   GGR    
Sbjct: 721 ASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSHEVGGRKSKK 780

Query: 781 -KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVRASFTT 828
            KSRKPKCCICYNM+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCRAPIEDVV+AS + 
Sbjct: 781 DKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVVQASLSA 805

BLAST of Lsi01G000070 vs. ExPASy TrEMBL
Match: A0A0A0KL44 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G538730 PE=4 SV=1)

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 692/842 (82.19%), Postives = 737/842 (87.53%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             N+NPNP+LANF V KTNH+TTT    +RSDDS+QHTS TNDPQTQT STPTP   PIP
Sbjct: 61  --NKNPNPVLANFPVTKTNHDTTT----KRSDDSQQHTS-TNDPQTQTTSTPTP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQ 180
           ET +NKNQTSKLGASSLVQIWEKRLNVSSSN+GLNANANA N  VCS KQETE       
Sbjct: 121 ETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANA-NTAVCSTKQETEA------ 180

Query: 181 EQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240
           EQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPP STQSQSSDAGERERVRV
Sbjct: 181 EQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRV 240

Query: 241 VDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSPRIRGR 300
           VDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIPRDQVE +CLSHILCSPRIRGR
Sbjct: 241 VDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGR 300

Query: 301 QAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360
           QAFADLLLQIERDRQRELDILV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF
Sbjct: 301 QAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360

Query: 361 VITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHT-PDPHATN 420
           VITPRENHRSS+IMHLRERFSGV ++ A+S  GEMLNN+D+DK QS TNA+T    H  +
Sbjct: 361 VITPRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPH 420

Query: 421 VTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPEREQEQEE 480
           V N  EKD   DNDNQQ VVG+HS + D ++QI+G FKEEQIE+QE  + +P  +QE E 
Sbjct: 421 VINTNEKD--KDNDNQQ-VVGMHSNNTD-DDQILGGFKEEQIEKQEQKQQKP--DQELEP 480

Query: 481 EGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDWFSD 540
           E G EVD PS EGIWQD   LN DSQDS++GWEAE Q SEA EESYGADYVGTSYDWF+D
Sbjct: 481 EQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQ-SEAGEESYGADYVGTSYDWFAD 540

Query: 541 ISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTRLER 600
           ISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLMVTRLER
Sbjct: 541 ISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLER 600

Query: 601 QTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE--DDERSLISAQYHEASDY 660
           QT QEEEY    EDDD   +EE+WCFSEGHTQ KSSDNEEE  DDERSLISAQYHEASDY
Sbjct: 601 QTHQEEEYNEVKEDDD--VDEELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDY 660

Query: 661 LDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQRSSLRS 720
           LDQSASPLQLASPSILSSWSY    EMGEDSNRG S SSPQP QPQFSS NNQQR SL S
Sbjct: 661 LDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVS 720

Query: 721 TTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR-NEVGGGR--- 780
            +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR +EVGG +   
Sbjct: 721 ASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKK 780

Query: 781 -KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVRASFTT 828
            KSRKPKCCICY+M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCR+PIEDVV+ASF+T
Sbjct: 781 DKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRSPIEDVVQASFST 810

BLAST of Lsi01G000070 vs. ExPASy TrEMBL
Match: A0A5A7U932 (Altered inheritance of mitochondria protein 44 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00080 PE=4 SV=1)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 692/848 (81.60%), Postives = 733/848 (86.44%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             NQN NP+L NF V KTNH+T+T    +RSDDS+QH S TNDPQTQT STP P   PIP
Sbjct: 61  --NQNTNPVLTNFPVTKTNHDTST----KRSDDSQQHAS-TNDPQTQTTSTPAP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANA-----TNATVCSGKQETETE 180
           ET  NKNQTSKLGASSLVQIWEKRLNV SSN+GLNANANA      N  VCS KQETE  
Sbjct: 121 ETSPNKNQTSKLGASSLVQIWEKRLNV-SSNIGLNANANANANANANTAVCSTKQETEA- 180

Query: 181 QEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240
              EQEQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPPSSTQSQSSDAGER
Sbjct: 181 ---EQEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSP 300
           ERVRVVDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIP+DQVEP+CLSHILCSP
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSP 300

Query: 301 RIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360
           RIRGRQAFADLLLQIERDRQRELD+LV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ
Sbjct: 301 RIRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360

Query: 361 KRPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNA--HTP 420
           KRPQFVITPRENHRS +IMHLRERFSGVGEN A+S  GEMLN+ND+DK Q  TNA  HTP
Sbjct: 361 KRPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHTP 420

Query: 421 DPHATNVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPER 480
            PH  N TN+K+K    DNDNQQ VVG+HS + D N+QI+G FKEEQIE+QE  KP+ E 
Sbjct: 421 -PHVIN-TNEKDK----DNDNQQ-VVGIHSNNTD-NDQIIGGFKEEQIEKQE-QKPDQEL 480

Query: 481 EQEQEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTS 540
           E EQ    G EVDPPS EGIWQD   LN DS DS++GWEAE Q SEA EESYGADYVGTS
Sbjct: 481 EPEQ----GGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQ-SEAGEESYGADYVGTS 540

Query: 541 YDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLM 600
           YDWF+DISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLM
Sbjct: 541 YDWFADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLM 600

Query: 601 VTRLERQTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE--DDERSLISAQY 660
           VTRLERQTQQEEEY    EDDD   +EE+WCFSEGHTQPKSSDNEEE  DDERSLI+AQY
Sbjct: 601 VTRLERQTQQEEEYNEVKEDDD--VDEELWCFSEGHTQPKSSDNEEEDDDDERSLITAQY 660

Query: 661 HEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQ 720
           HEASDYLDQSASPLQLASPSILSSWSY    +MGEDSNRG S SSPQP  PQFSS NNQ 
Sbjct: 661 HEASDYLDQSASPLQLASPSILSSWSY----DMGEDSNRGTSISSPQPLLPQFSSNNNQH 720

Query: 721 RSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNEVGG 780
           R SL S +H+PSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR+   G
Sbjct: 721 RPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSHEVG 780

Query: 781 GR-----KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVV 828
           GR     KSRKPKCCICYNM+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCRAPIEDVV
Sbjct: 781 GRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVV 811

BLAST of Lsi01G000070 vs. ExPASy TrEMBL
Match: A0A6J1ECL3 (trichohyalin-like OS=Cucurbita moschata OX=3662 GN=LOC111432973 PE=4 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 676/862 (78.42%), Postives = 726/862 (84.22%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASS+VEISSS+SPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDC+TITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQ-TQTNSTPTPTPIPI 120
           NQN NPNP++ NFRVP+TNH    ++ PRRS+  +  ++  N PQ    N+ P     P 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNH----DSAPRRSNPCQTLSTIINHPQNNNNNNNPQTRTTPT 120

Query: 121 PETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNAT--VCSGKQET--ETE 180
           P+TGT+KN +SKLGASSLVQIWEKRLNVSSSNVGLNANANA NAT  VCS KQET  E E
Sbjct: 121 PQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANA-NATPSVCSVKQETEQEQE 180

Query: 181 QEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240
           QEQEQEQEQACS+ AGDF DERYDAG GSED FADWHSSRTSSSSPPSSTQSQ SDA ER
Sbjct: 181 QEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQSQISDARER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNE-SSHPTLIPRDQVEPRCLSHILCSPR 300
           ERVRVVDIIRRLTLTAAKPPHSSWVED NDH NE SS+PTLI R QVEP+CLSHIL SPR
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVED-NDHSNESSSNPTLILRYQVEPKCLSHILYSPR 300

Query: 301 IRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQK 360
           IRGRQAFADLLLQIERDRQREL+ LV+RRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQK
Sbjct: 301 IRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQK 360

Query: 361 RPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHTPDPH 420
           RP+FVITPR NHR+ +I HLRERFSG GEN ARS IGEML+NND+DKNQ  T+ HT   H
Sbjct: 361 RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDPHT---H 420

Query: 421 ATNVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIE--EQEPAKPEPERE 480
           ATN T D + DN  D++NQQ VVG+        N I   F EE+IE  E+EPA+ EPE E
Sbjct: 421 ATN-TKDNDNDNDKDSNNQQ-VVGI--------NPIPEHFNEEEIEEKEEEPAQ-EPEPE 480

Query: 481 QEQEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSY 540
           QEQ      EVDPPSSEG WQDRP+LNLDSQDSI+GWEAED  SEAAEESY  +Y+GTSY
Sbjct: 481 QEQ------EVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDH-SEAAEESYDENYLGTSY 540

Query: 541 DWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMV 600
           DWF+DISRPRSYWEDRR+SWYQQML+SNSAN+EIRQLIERKTVSNFLSS+FRERMDKLMV
Sbjct: 541 DWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMV 600

Query: 601 TRLERQTQQEEEYEDDDGAE-------------EEEMWCFSEGHTQPKSSDNEEED---- 660
           +RLERQTQQEEEY  DDGAE             EEE+WCFSEGHTQPKSSDNEEE+    
Sbjct: 601 SRLERQTQQEEEY--DDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPKSSDNEEEEEEEE 660

Query: 661 ---DERSLI-SAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSP 720
              DERSLI SAQY EASD LD SASPLQ  SPSILSSWSYQLDNEMGEDSNRGASTSSP
Sbjct: 661 EDRDERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSP 720

Query: 721 QPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 780
           QPFQPQFSSN  QRSS  STTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL
Sbjct: 721 QPFQPQFSSNT-QRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 780

Query: 781 MLQHSIKRNEVGGG------RKSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRG 828
           MLQHSIKR+EVGGG       KSRK KCCICY+MQIDSLLYRCGHMCSCMKCAKELQWRG
Sbjct: 781 MLQHSIKRHEVGGGGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRG 832

BLAST of Lsi01G000070 vs. ExPASy TrEMBL
Match: A0A6J1IHV7 (uncharacterized protein LOC111477598 OS=Cucurbita maxima OX=3661 GN=LOC111477598 PE=4 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 665/852 (78.05%), Postives = 716/852 (84.04%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASS+VEISSS+SPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDC+TITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQ-TQTNSTPTPTPIPI 120
           NQN NPNP++ NFR P+TNH    ++ PRRS+  +  ++  N PQ    N+ P     P 
Sbjct: 61  NQNHNPNPVIPNFRGPRTNH----DSAPRRSNPCQTLSTIINHPQNNNNNNNPQIRTTPT 120

Query: 121 PETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNAT--VCSGKQETETEQE 180
           PETGT+KN +SKL ASSLVQIWEKRLNVSSSNVGLNANANA NAT  VCS KQET     
Sbjct: 121 PETGTDKNHSSKL-ASSLVQIWEKRLNVSSSNVGLNANANA-NATPSVCSVKQET----- 180

Query: 181 QEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERER 240
            EQEQEQACS+ AGDF DERYDAG GSEDGFADWHSSRTSSSSPPSSTQSQ SDA ERER
Sbjct: 181 -EQEQEQACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDARERER 240

Query: 241 VRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNE-SSHPTLIPRDQVEPRCLSHILCSPRIR 300
           VRVVDIIRRLTLTAAKPPHSSWVED NDH +E SS+PTLI R QVEP+CLSHIL SPRIR
Sbjct: 241 VRVVDIIRRLTLTAAKPPHSSWVED-NDHSSESSSNPTLILRYQVEPKCLSHILYSPRIR 300

Query: 301 GRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRP 360
           GRQAFADLLLQIERDRQREL+ LV+RRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQKRP
Sbjct: 301 GRQAFADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQKRP 360

Query: 361 QFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHTPDPHAT 420
           +FVITPR NHR+ +I HLRERFSG GEN ARS IGEML+NND+DKNQ  T+AHT   HAT
Sbjct: 361 KFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDAHT---HAT 420

Query: 421 NVTNDKEKDNQNDND-NQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPA-KPEPEREQE 480
           N  ND + DN ND D N Q VVG+        N I  DF EE+IEEQEP  +P PE    
Sbjct: 421 N-ANDNDNDNDNDKDSNNQQVVGI--------NPIPEDFNEEEIEEQEPVQEPVPE---- 480

Query: 481 QEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDW 540
                  EVDPPSSEG WQDRP+LNLDSQDSI+GWEAED  SEAAEESY  +Y+GTSYDW
Sbjct: 481 ------PEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDH-SEAAEESYDENYLGTSYDW 540

Query: 541 FSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTR 600
           F+DISRPRSYWEDRR+SWYQQML+SNSAN+EIRQLIERKTVSNFLSS+FRERMDKLMV+R
Sbjct: 541 FADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSR 600

Query: 601 LERQTQQEEEYED-------DDGAEEEEMWCFSEGHTQPKSSDNEEED-----DERSLI- 660
           LERQTQQEEEY+D       ++  EEEE+WCFSEGHTQP+SSDNEEE+     DERSLI 
Sbjct: 601 LERQTQQEEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPESSDNEEEEEEEDRDERSLIS 660

Query: 661 SAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSN 720
           SA Y EASD LDQSASPLQ  SPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSN
Sbjct: 661 SAPYQEASDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSN 720

Query: 721 NQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNE 780
             QRSS  STTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR+E
Sbjct: 721 T-QRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRHE 780

Query: 781 VGGG------RKSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPI 828
           VGGG       KSRK KCCICY+MQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVC API
Sbjct: 781 VGGGGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPI 815

BLAST of Lsi01G000070 vs. NCBI nr
Match: XP_038877490.1 (putative uncharacterized protein DDB_G0271606 [Benincasa hispida])

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 735/852 (86.27%), Postives = 767/852 (90.02%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITP-- 60
           MASSQVEISSSSSPFGCVLRDHNRRREPNV ATHVARFR+NLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVAATHVARFRDNLKTLVMDRLNDCITITPNK 60

Query: 61  ----NQNQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTP 120
               NQNQNQNPNP+L NFRV KTNHNTTTNA PRR++DS++ TS  NDPQTQT +  T 
Sbjct: 61  NKNKNQNQNQNPNPVLPNFRVSKTNHNTTTNAAPRRANDSQRQTS-INDPQTQTQTQTTS 120

Query: 121 TPIPIPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETET 180
           T  P PETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANA+N  VCS KQETET
Sbjct: 121 T--PTPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANASNTAVCSAKQETET 180

Query: 181 EQEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGE 240
                 EQEQACSV  GDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGE
Sbjct: 181 ------EQEQACSVEVGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGE 240

Query: 241 RERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNE---SSHPTLIPRDQVEP-RCLSHIL 300
           RERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNE   SSHPTLI RDQVEP RCLSHIL
Sbjct: 241 RERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSSSSHPTLILRDQVEPARCLSHIL 300

Query: 301 CSPRIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALE 360
           CSPRIRGRQAFADLLLQIERDRQRELDILV+RRAVSKF QRGRIQSLLRLKILKRGMALE
Sbjct: 301 CSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFSQRGRIQSLLRLKILKRGMALE 360

Query: 361 DEQKRPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHT 420
           DEQKRPQFVITPRENHRSS+IMHLRE+FSGVGEN ARS IGEMLNNNDEDKNQSHTNAHT
Sbjct: 361 DEQKRPQFVITPRENHRSSTIMHLREKFSGVGENGARSPIGEMLNNNDEDKNQSHTNAHT 420

Query: 421 PDPHATNVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQE-----PA 480
           P PHATN TN+KEK    DNDN+Q VVG+HS SI NN+QI+  FKEEQIEE+E       
Sbjct: 421 P-PHATN-TNEKEK----DNDNEQ-VVGIHSNSI-NNDQILEGFKEEQIEEREREQEQEQ 480

Query: 481 KPEPEREQE--QEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESY 540
           K E E+EQE  QE+E G EVDPPSSEG WQDRP+LNLDSQDSI+GWEAEDQ SEAAEESY
Sbjct: 481 KQEIEQEQEPKQEQEQGEEVDPPSSEGTWQDRPNLNLDSQDSINGWEAEDQ-SEAAEESY 540

Query: 541 GADYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDF 600
           GADYVGTSYDWFSDISRPRSYWEDRRQSWYQQML+S+SANDEIRQLI+RKTVS FLSSDF
Sbjct: 541 GADYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLDSSSANDEIRQLIQRKTVSTFLSSDF 600

Query: 601 RERMDKLMVTRLERQTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDN-EEEDDER 660
           RERMDKLMVTRLERQTQ+EEEY    E+DD    EE+WCFSEG TQ KSSDN EEE+DER
Sbjct: 601 RERMDKLMVTRLERQTQEEEEYDEVNEEDDDDRAEELWCFSEGRTQSKSSDNGEEEEDER 660

Query: 661 SLISAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF 720
           SLISAQYHEASDYLDQS SPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF
Sbjct: 661 SLISAQYHEASDYLDQSTSPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQF 720

Query: 721 SSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK 780
           SSNNQQ SSL STTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK
Sbjct: 721 SSNNQQGSSLVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIK 780

Query: 781 RNEVGGGR-----KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRA 826
           R+EVGGGR     KSRKPKCCICYNM+IDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRA
Sbjct: 781 RHEVGGGRRSKKEKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRA 834

BLAST of Lsi01G000070 vs. NCBI nr
Match: XP_008439420.1 (PREDICTED: uncharacterized protein LOC103484234 [Cucumis melo])

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 693/842 (82.30%), Postives = 734/842 (87.17%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             NQN NP+L NF V KTNH+T+T    +RSDDS+QH S TNDPQTQT STP P   PIP
Sbjct: 61  --NQNTNPVLTNFPVTKTNHDTST----KRSDDSQQHAS-TNDPQTQTTSTPAP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQ 180
           ET  NKNQTSKLGASSLVQIWEKRLNVSSSN+GLNANANA N  VCS KQETE     EQ
Sbjct: 121 ETSPNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANA-NTAVCSTKQETEA----EQ 180

Query: 181 EQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240
           EQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV
Sbjct: 181 EQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240

Query: 241 VDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSPRIRGR 300
           VDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIP+DQVEP+CLSHILCSPRIRGR
Sbjct: 241 VDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGR 300

Query: 301 QAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360
           QAFADLLLQIERDRQRELD+LV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF
Sbjct: 301 QAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360

Query: 361 VITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNA--HTPDPHAT 420
           VITPRENHRS +IMHLRERFSGVGEN A+S  GEMLN+ND+DK Q  TNA  HTP PH  
Sbjct: 361 VITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHTP-PHVI 420

Query: 421 NVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPEREQEQE 480
           N TN+K+K    DNDNQQ VVG+HS + D N+QI+G FKEEQIE+QE  KP+ E E EQ 
Sbjct: 421 N-TNEKDK----DNDNQQ-VVGIHSNNTD-NDQIIGGFKEEQIEKQE-QKPDQELEPEQ- 480

Query: 481 EEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDWFS 540
              G EVDPPS EGIWQD   LN DS DS++GWEAE Q SEA EESYGADYVGTSYDWF+
Sbjct: 481 ---GGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQ-SEAGEESYGADYVGTSYDWFA 540

Query: 541 DISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTRLE 600
           DISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLMVTRLE
Sbjct: 541 DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLE 600

Query: 601 RQTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE-DDERSLISAQYHEASDY 660
           RQTQQEEEY    EDDD   +EE+WCFSEGHTQPKSSDNEEE DDERSLI+AQYHEASDY
Sbjct: 601 RQTQQEEEYNEVKEDDD--VDEELWCFSEGHTQPKSSDNEEEDDDERSLITAQYHEASDY 660

Query: 661 LDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQRSSLRS 720
           LDQSASPLQLASPSILSSWSY    +MGEDSNRG S SSPQP  PQFSS NNQ R SL S
Sbjct: 661 LDQSASPLQLASPSILSSWSY----DMGEDSNRGTSISSPQPLLPQFSSNNNQHRPSLVS 720

Query: 721 TTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNEVGGGR---- 780
            +H+PSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR+   GGR    
Sbjct: 721 ASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSHEVGGRKSKK 780

Query: 781 -KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVRASFTT 828
            KSRKPKCCICYNM+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCRAPIEDVV+AS + 
Sbjct: 781 DKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVVQASLSA 805

BLAST of Lsi01G000070 vs. NCBI nr
Match: XP_004134880.1 (uncharacterized protein LOC101206495 [Cucumis sativus] >KGN49544.1 hypothetical protein Csa_003486 [Cucumis sativus])

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 692/842 (82.19%), Postives = 737/842 (87.53%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             N+NPNP+LANF V KTNH+TTT    +RSDDS+QHTS TNDPQTQT STPTP   PIP
Sbjct: 61  --NKNPNPVLANFPVTKTNHDTTT----KRSDDSQQHTS-TNDPQTQTTSTPTP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQ 180
           ET +NKNQTSKLGASSLVQIWEKRLNVSSSN+GLNANANA N  VCS KQETE       
Sbjct: 121 ETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANA-NTAVCSTKQETEA------ 180

Query: 181 EQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240
           EQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPP STQSQSSDAGERERVRV
Sbjct: 181 EQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRV 240

Query: 241 VDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSPRIRGR 300
           VDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIPRDQVE +CLSHILCSPRIRGR
Sbjct: 241 VDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGR 300

Query: 301 QAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360
           QAFADLLLQIERDRQRELDILV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF
Sbjct: 301 QAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQF 360

Query: 361 VITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHT-PDPHATN 420
           VITPRENHRSS+IMHLRERFSGV ++ A+S  GEMLNN+D+DK QS TNA+T    H  +
Sbjct: 361 VITPRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPH 420

Query: 421 VTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPEREQEQEE 480
           V N  EKD   DNDNQQ VVG+HS + D ++QI+G FKEEQIE+QE  + +P  +QE E 
Sbjct: 421 VINTNEKD--KDNDNQQ-VVGMHSNNTD-DDQILGGFKEEQIEKQEQKQQKP--DQELEP 480

Query: 481 EGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDWFSD 540
           E G EVD PS EGIWQD   LN DSQDS++GWEAE Q SEA EESYGADYVGTSYDWF+D
Sbjct: 481 EQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQ-SEAGEESYGADYVGTSYDWFAD 540

Query: 541 ISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTRLER 600
           ISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLMVTRLER
Sbjct: 541 ISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLER 600

Query: 601 QTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE--DDERSLISAQYHEASDY 660
           QT QEEEY    EDDD   +EE+WCFSEGHTQ KSSDNEEE  DDERSLISAQYHEASDY
Sbjct: 601 QTHQEEEYNEVKEDDD--VDEELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDY 660

Query: 661 LDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQRSSLRS 720
           LDQSASPLQLASPSILSSWSY    EMGEDSNRG S SSPQP QPQFSS NNQQR SL S
Sbjct: 661 LDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVS 720

Query: 721 TTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR-NEVGGGR--- 780
            +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR +EVGG +   
Sbjct: 721 ASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKK 780

Query: 781 -KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVRASFTT 828
            KSRKPKCCICY+M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCR+PIEDVV+ASF+T
Sbjct: 781 DKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRSPIEDVVQASFST 810

BLAST of Lsi01G000070 vs. NCBI nr
Match: KAA0052413.1 (altered inheritance of mitochondria protein 44 [Cucumis melo var. makuwa])

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 692/848 (81.60%), Postives = 733/848 (86.44%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDC+TITP  
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITP-- 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIP 120
             NQN NP+L NF V KTNH+T+T    +RSDDS+QH S TNDPQTQT STP P   PIP
Sbjct: 61  --NQNTNPVLTNFPVTKTNHDTST----KRSDDSQQHAS-TNDPQTQTTSTPAP---PIP 120

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANA-----TNATVCSGKQETETE 180
           ET  NKNQTSKLGASSLVQIWEKRLNV SSN+GLNANANA      N  VCS KQETE  
Sbjct: 121 ETSPNKNQTSKLGASSLVQIWEKRLNV-SSNIGLNANANANANANANTAVCSTKQETEA- 180

Query: 181 QEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240
              EQEQEQACSV AGDFEDERYDAGPGS+DGFADWHSSRTSSSSPPSSTQSQSSDAGER
Sbjct: 181 ---EQEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESS--HPTLIPRDQVEPRCLSHILCSP 300
           ERVRVVDIIRRLTLTAAKPPHSSWVEDHND PNESS  HPTLIP+DQVEP+CLSHILCSP
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSP 300

Query: 301 RIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360
           RIRGRQAFADLLLQIERDRQRELD+LV+RRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ
Sbjct: 301 RIRGRQAFADLLLQIERDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360

Query: 361 KRPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNA--HTP 420
           KRPQFVITPRENHRS +IMHLRERFSGVGEN A+S  GEMLN+ND+DK Q  TNA  HTP
Sbjct: 361 KRPQFVITPRENHRSFTIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHTP 420

Query: 421 DPHATNVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEPAKPEPER 480
            PH  N TN+K+K    DNDNQQ VVG+HS + D N+QI+G FKEEQIE+QE  KP+ E 
Sbjct: 421 -PHVIN-TNEKDK----DNDNQQ-VVGIHSNNTD-NDQIIGGFKEEQIEKQE-QKPDQEL 480

Query: 481 EQEQEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTS 540
           E EQ    G EVDPPS EGIWQD   LN DS DS++GWEAE Q SEA EESYGADYVGTS
Sbjct: 481 EPEQ----GGEVDPPSLEGIWQDGSKLNSDSHDSMNGWEAEYQ-SEAGEESYGADYVGTS 540

Query: 541 YDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLM 600
           YDWF+DISRPRSYWEDRRQSWYQQML+SNSANDEIRQLI+RKTVSNFLSSDFRERMDKLM
Sbjct: 541 YDWFADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLM 600

Query: 601 VTRLERQTQQEEEY----EDDDGAEEEEMWCFSEGHTQPKSSDNEEE--DDERSLISAQY 660
           VTRLERQTQQEEEY    EDDD   +EE+WCFSEGHTQPKSSDNEEE  DDERSLI+AQY
Sbjct: 601 VTRLERQTQQEEEYNEVKEDDD--VDEELWCFSEGHTQPKSSDNEEEDDDDERSLITAQY 660

Query: 661 HEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSS-NNQQ 720
           HEASDYLDQSASPLQLASPSILSSWSY    +MGEDSNRG S SSPQP  PQFSS NNQ 
Sbjct: 661 HEASDYLDQSASPLQLASPSILSSWSY----DMGEDSNRGTSISSPQPLLPQFSSNNNQH 720

Query: 721 RSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNEVGG 780
           R SL S +H+PSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR+   G
Sbjct: 721 RPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRSHEVG 780

Query: 781 GR-----KSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVV 828
           GR     KSRKPKCCICYNM+IDSLLYRCGHMCSCMKC KELQWRGGKCPVCRAPIEDVV
Sbjct: 781 GRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRAPIEDVV 811

BLAST of Lsi01G000070 vs. NCBI nr
Match: XP_022925581.1 (trichohyalin-like [Cucurbita moschata] >KAG7034825.1 Protein neuralized, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 676/862 (78.42%), Postives = 726/862 (84.22%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQ 60
           MASS+VEISSS+SPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDC+TITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  NQNQNPNPILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQ-TQTNSTPTPTPIPI 120
           NQN NPNP++ NFRVP+TNH    ++ PRRS+  +  ++  N PQ    N+ P     P 
Sbjct: 61  NQNHNPNPVIPNFRVPRTNH----DSAPRRSNPCQTLSTIINHPQNNNNNNNPQTRTTPT 120

Query: 121 PETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNAT--VCSGKQET--ETE 180
           P+TGT+KN +SKLGASSLVQIWEKRLNVSSSNVGLNANANA NAT  VCS KQET  E E
Sbjct: 121 PQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANA-NATPSVCSVKQETEQEQE 180

Query: 181 QEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGER 240
           QEQEQEQEQACS+ AGDF DERYDAG GSED FADWHSSRTSSSSPPSSTQSQ SDA ER
Sbjct: 181 QEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQSQISDARER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNE-SSHPTLIPRDQVEPRCLSHILCSPR 300
           ERVRVVDIIRRLTLTAAKPPHSSWVED NDH NE SS+PTLI R QVEP+CLSHIL SPR
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVED-NDHSNESSSNPTLILRYQVEPKCLSHILYSPR 300

Query: 301 IRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQK 360
           IRGRQAFADLLLQIERDRQREL+ LV+RRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQK
Sbjct: 301 IRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQK 360

Query: 361 RPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNAHTPDPH 420
           RP+FVITPR NHR+ +I HLRERFSG GEN ARS IGEML+NND+DKNQ  T+ HT   H
Sbjct: 361 RPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDPHT---H 420

Query: 421 ATNVTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIE--EQEPAKPEPERE 480
           ATN T D + DN  D++NQQ VVG+        N I   F EE+IE  E+EPA+ EPE E
Sbjct: 421 ATN-TKDNDNDNDKDSNNQQ-VVGI--------NPIPEHFNEEEIEEKEEEPAQ-EPEPE 480

Query: 481 QEQEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSY 540
           QEQ      EVDPPSSEG WQDRP+LNLDSQDSI+GWEAED  SEAAEESY  +Y+GTSY
Sbjct: 481 QEQ------EVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDH-SEAAEESYDENYLGTSY 540

Query: 541 DWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMV 600
           DWF+DISRPRSYWEDRR+SWYQQML+SNSAN+EIRQLIERKTVSNFLSS+FRERMDKLMV
Sbjct: 541 DWFADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMV 600

Query: 601 TRLERQTQQEEEYEDDDGAE-------------EEEMWCFSEGHTQPKSSDNEEED---- 660
           +RLERQTQQEEEY  DDGAE             EEE+WCFSEGHTQPKSSDNEEE+    
Sbjct: 601 SRLERQTQQEEEY--DDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPKSSDNEEEEEEEE 660

Query: 661 ---DERSLI-SAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSP 720
              DERSLI SAQY EASD LD SASPLQ  SPSILSSWSYQLDNEMGEDSNRGASTSSP
Sbjct: 661 EDRDERSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSP 720

Query: 721 QPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 780
           QPFQPQFSSN  QRSS  STTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL
Sbjct: 721 QPFQPQFSSNT-QRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 780

Query: 781 MLQHSIKRNEVGGG------RKSRKPKCCICYNMQIDSLLYRCGHMCSCMKCAKELQWRG 828
           MLQHSIKR+EVGGG       KSRK KCCICY+MQIDSLLYRCGHMCSCMKCAKELQWRG
Sbjct: 781 MLQHSIKRHEVGGGGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRG 832

BLAST of Lsi01G000070 vs. TAIR 10
Match: AT1G30860.1 (RING/U-box superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 7.9e-59
Identity = 234/754 (31.03%), Postives = 361/754 (47.88%), Query Frame = 0

Query: 121 ETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQ 180
           E+     +   L ASSLVQIWE RLN S+       N+ +++ +  S    +++    + 
Sbjct: 60  ESPLESKRNDNLVASSLVQIWEARLNRSN-----GGNSPSSSQSTASSSSRSDSGVSVQD 119

Query: 181 EQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRV 240
                 S   GD E              AD    RT      S +    SD+GE +  RV
Sbjct: 120 SGLSESSSIDGDSE-------------IAD----RTVEIE--SRSHGLVSDSGESKWGRV 179

Query: 241 VDIIRRLTLTAAKPPHSSWVEDHNDHPNESSHPTLIPRDQVEPRCLSH---------ILC 300
            D+IRRL+    K        D+             PR    P C S          +  
Sbjct: 180 ADLIRRLSNEDKK----RTAGDNGSGGGGGLEIIRTPR----PYCTSSSSEKSNFPVVSF 239

Query: 301 SPRIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALED 360
           SP+IRGRQAF D L+Q+ERDR RELD L +R AVS+F QRGR+QS+LRL+ L R + ++D
Sbjct: 240 SPKIRGRQAFTDFLMQMERDRHRELDSLFERNAVSRFTQRGRLQSMLRLRNLNRCLVIQD 299

Query: 361 EQKRPQFVITPRENHRSSSIMHLRERFSGVGENSARSL--------IGEMLNNN-DEDKN 420
             +              S+++HLRE+F     NSA +           EM N + +E + 
Sbjct: 300 RNRSNAKTTGLNRIESGSAVLHLREKFHANVVNSAAATDKRKNHQRSTEMNNKSVEETEG 359

Query: 421 QSHTNAHTPDPHAT-----------------NVTNDKEKDNQNDN-DNQQVVVGVHSTSI 480
            + T+    D   T                   T  KE+++ +   D+Q+  + +  T  
Sbjct: 360 TTTTSTRLKDGGVTLEAFFAERLRPRNRKIEEATLCKEEESVSGTVDSQKNCLQLQETR- 419

Query: 481 DNNNQIVGDFKEEQIEEQEPAKPE-------PEREQEQEEEGGSEVDPPSSEGIWQDRPD 540
               +   D K E+ EE+  +  E         + Q++   G    +   +EG+  +  +
Sbjct: 420 -EVEETCNDGKSEKKEEENTSPSEYLSRESRLRQNQDENNVGKYMQETRETEGLVHESNE 479

Query: 541 LN--LDSQDS--IDGWEAEDQISEAAEESYGADYVGTSYDWFSDISRPRSYWEDRRQSWY 600
           ++  LD Q++  ++ W  +++ ++  E+SY  +Y   + DW S+I+RPRSYWE+ R+S Y
Sbjct: 480 MDQCLDQQETSYLNRWGEQEEYAD--EQSYYGEY---NDDWLSEIARPRSYWEELRKSRY 539

Query: 601 QQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYEDDDGAEE 660
            +++ + S  D+I +L+ER+TV++FL S  RE++D LM++R++  + +  +  +    EE
Sbjct: 540 LEVMNTRSEKDDICRLLERRTVTDFLDSGLREKIDNLMMSRVQTHSDKHSKKWELQQEEE 599

Query: 661 EEMWCFSEGHTQPKSSDNEEEDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYQ 720
           EE+           + + +EE  E  L   +  +  D L QS+S   +++ S   SWS Q
Sbjct: 600 EEV-----------NFEIDEEIKEEPLRGGEEQDDRDDLSQSSSS-HISASSPAGSWSSQ 659

Query: 721 LDNEMGEDSNRGASTSSPQPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLY 780
            D ++         TS+     P     N Q           S EMELI  +R  ++QL 
Sbjct: 660 -DTDV---------TST-----PALVVQNPQ-----------SPEMELISGMRSQIQQLQ 719

Query: 781 QEMSELRKSIKCCMDMQLMLQHSIKRNEVGGGRKSRKPKCCICYNMQIDSLLYRCGHMCS 828
           QEMS LR S+K C+D    LQH   +          K KCC+C   Q++++LYRCGHMC 
Sbjct: 720 QEMSVLRDSVKTCLDANASLQHKAHQ------ENPMKRKCCVCDETQVEAVLYRCGHMCM 730

BLAST of Lsi01G000070 vs. TAIR 10
Match: AT2G34920.1 (RING/U-box superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 8.8e-58
Identity = 255/826 (30.87%), Postives = 371/826 (44.92%), Query Frame = 0

Query: 8   ISSSSSPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCMTITPNQNQNQNPN 67
           ++S    FG VLRD N+R   +V       F+ NLK  V                     
Sbjct: 1   MASLRPSFGSVLRDRNQRHNDDVV------FKKNLKAQV--------------------- 60

Query: 68  PILANFRVPKTNHNTTTNAVPRRSDDSRQHTSTTNDPQTQTNSTPTPTPIPIPETGTNKN 127
                         T   A+   S ++R  +   N  +    S P     P    G N +
Sbjct: 61  -------------KTAPPAISDESSENRVDSLIGNKRKKNNKSRPGSPEKPRTRKGNNFS 120

Query: 128 QTSKLGASSLVQIWEKRLNVSSSNVGLNANANATNATVCSGKQETETEQEQEQEQEQACS 187
                GASSLVQIWE RLN           +N  N+ + S   E  +E   ++    A S
Sbjct: 121 DNLG-GASSLVQIWEARLN----------RSNGGNSAIHSQSIEISSEASVQEIHLLAPS 180

Query: 188 VAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDIIRRL 247
           +   D E E                 S   S SP  + + +S          V DIIRRL
Sbjct: 181 I---DGESE-----------------SENESKSPDQTVEIESGTLNS-----VSDIIRRL 240

Query: 248 T----LTAAKPPHSSWVEDHNDHPNESSHPTLIPRDQVEPRCLSHILCSPRIRGRQAFAD 307
           +    LTA+    +       D P       ++    +E      + CSPRIRGRQA++D
Sbjct: 241 SNEQKLTASNNGGAV------DMP-------IVKTPTLEKSSFQVVTCSPRIRGRQAYSD 300

Query: 308 LLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPR 367
           LL+ +ER+R REL+ L+ R AVS+FPQRGR+QS+LRL+ LKRG+A++D  +      T  
Sbjct: 301 LLVHLERERHRELESLLGRNAVSRFPQRGRLQSMLRLRSLKRGLAIQDRHRG----TTKS 360

Query: 368 ENHR---SSSIMHLRERFSGVGENSARSL---IGEMLNNNDEDKNQSHTNAHTPDPHATN 427
           + +R   SS+I+HLRE+      N+A       G+      E      T+     P    
Sbjct: 361 DLNRFQPSSTILHLREKLREKAANAAAEAGLKKGQQSTVETESMQSKETSGILHSPSTER 420

Query: 428 VTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKEEQIEEQEP---AKPEPEREQE 487
           ++   +K N  +   ++       + +     IV +  E + +   P     P+  R   
Sbjct: 421 LS--PQKRNIEEAILRKNETETKMSYLQLKKAIVAEVLERESDNTSPLTSVTPQEPRILR 480

Query: 488 QEEEGGSEVDPPSSEGIWQDRPDLNLDSQDSIDGWEAEDQISEAAEESYGADYVGTSYDW 547
            EE G  E     +EG  Q+ P L         GWE +++  +  E+SY   Y   SYDW
Sbjct: 481 NEEAGKLE---SGTEGT-QETPFLETQEMSFQSGWEEQEEYED--EQSY---YGDMSYDW 540

Query: 548 FSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIERKTVSNFLSSDFRERMDKLMVTR 607
           F++ISRPR+YWED R+S Y +++ + S  D+I +L+ER+TVS FL S  RE++DKL+++R
Sbjct: 541 FTEISRPRTYWEDLRKSRYLEVMNTKSDKDDICRLLERRTVSGFLQSGLREKIDKLIMSR 600

Query: 608 LERQTQQEEEYEDDDGAEEEEMWCFSEGHTQPKSSDNEEEDDERSLISAQYHEASDYLDQ 667
           +    Q    +  ++  +EEE           K    EE+D++R           D L Q
Sbjct: 601 V----QIHPAHRIEEATKEEE-----------KYDIGEEKDEDR-----------DDLSQ 645

Query: 668 SASPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSNNQQRSSLRSTTHH 727
           S+S  Q+ +PS   SWS  LD  +         TS+P                    TH+
Sbjct: 661 SSS--QIFAPSPAGSWS-SLDTGV---------TSTP--------------------THN 645

Query: 728 PSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKRNEVGGGRKSRKPKCC 787
               ++L             EMSELR S+K C+D+   LQ S+            K KCC
Sbjct: 721 LHSTLQL-------------EMSELRDSVKTCLDVNASLQKSVHL------ENPFKRKCC 645

Query: 788 ICYNMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVR 821
           +C   Q+++LLYRCGHMC+C++CA ELQ+ GGKCP+C A I DVVR
Sbjct: 781 VCNETQVETLLYRCGHMCTCLRCANELQYNGGKCPICHAKILDVVR 645

BLAST of Lsi01G000070 vs. TAIR 10
Match: AT5G04460.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.5e-33
Identity = 195/691 (28.22%), Postives = 304/691 (43.99%), Query Frame = 0

Query: 175 EQEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGE 234
           +      +EQ+  +  G+ E  R+ A  G  D   + HSS         + + +     E
Sbjct: 229 DNNNTSSREQSPDLGDGERERVRHIA-RGWMDSRINDHSSNVRQRD--DNRRPEWLGDTE 288

Query: 235 RERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSHPTLIPR-DQVE-PRCLSHILCS 294
           RERVR++    ++T        ++  ED         +    P+ D+V     ++H    
Sbjct: 289 RERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRVRVGLAVNHEEGQ 348

Query: 295 P--------RIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILK 354
           P        R+RGRQA  DLL++ ER+RQREL  L++ RAVS F  R RIQSLLR + L+
Sbjct: 349 PPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHRNRIQSLLRGRFLR 408

Query: 355 RGMALEDEQKRPQFVITPRENHRSSSIMHLRER--FSGVGE---NSARSLIGEMLNNNDE 414
                  E+       TP  +  S  ++ LRER   SG+ E   N   +++ E  +N D 
Sbjct: 409 NERPTVPER-------TP--SMASRELLQLRERQTVSGLREGFHNGRENIVHENTSNTDN 468

Query: 415 DKNQSHTNAHTPDPHATN---VTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKE 474
           D + + TNA      A N   VT++    ++  ND+  +     S   + +     D  +
Sbjct: 469 DNSNTSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESNLANADRDWEEDTNQ 528

Query: 475 EQI-EEQEPAKPEPEREQE--QEEEGGSEVDPPSSEGIWQD-RPDLNLDSQDSIDGWEAE 534
            +I +E  P    P  EQ    + +G    D    E    D   + +  + D     EA 
Sbjct: 529 RRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREASGFADDEYRTQEAH 588

Query: 535 DQISEAAEESYGADYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIER 594
               E +      ++ GT     S+  R R   + RR + +    + N  + E+R+L+ R
Sbjct: 589 GVWHENSSRQSDGNWPGTR----SEALRSRRVVQLRRLNRFHPPEDDNVYSMELRELLSR 648

Query: 595 KTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYEDDDGAEEEEMWCFSEGHTQPKSSDNE 654
           ++VSN L S FRE +D+L+ +  ER+     +++                + Q    D+ 
Sbjct: 649 RSVSNLLHSGFRESLDQLIQSYAERRGHTHVDWD-------------LHANLQTAIPDSP 708

Query: 655 EEDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEM-GEDSNRGASTSSP 714
           E D +  +                                + DN++ G + ++   T   
Sbjct: 709 ERDTDHQVF------------------------------VRNDNQLNGINGSQLLPTPPA 768

Query: 715 QPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 774
            P QP +  +    S  R + H   IE E++ DLRG + +L Q MS++++ ++ CMDMQL
Sbjct: 769 PPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEACMDMQL 828

Query: 775 MLQHSIKRNEVG-------------------GGRKSR--KPKCCICYNMQIDSLLYRCGH 822
            LQ S+ R EV                    G R S   K  CC+C +  ID+LLYRCGH
Sbjct: 829 ELQRSV-RQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDALLYRCGH 859

BLAST of Lsi01G000070 vs. TAIR 10
Match: AT5G04460.3 (RING/U-box superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.5e-33
Identity = 195/691 (28.22%), Postives = 304/691 (43.99%), Query Frame = 0

Query: 175 EQEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGE 234
           +      +EQ+  +  G+ E  R+ A  G  D   + HSS         + + +     E
Sbjct: 229 DNNNTSSREQSPDLGDGERERVRHIA-RGWMDSRINDHSSNVRQRD--DNRRPEWLGDTE 288

Query: 235 RERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSHPTLIPR-DQVE-PRCLSHILCS 294
           RERVR++    ++T        ++  ED         +    P+ D+V     ++H    
Sbjct: 289 RERVRIIREWMQMTSQQRGGARATPREDQRSTSEADRNHDAAPQVDRVRVGLAVNHEEGQ 348

Query: 295 P--------RIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLKILK 354
           P        R+RGRQA  DLL++ ER+RQREL  L++ RAVS F  R RIQSLLR + L+
Sbjct: 349 PPHVRRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHRNRIQSLLRGRFLR 408

Query: 355 RGMALEDEQKRPQFVITPRENHRSSSIMHLRER--FSGVGE---NSARSLIGEMLNNNDE 414
                  E+       TP  +  S  ++ LRER   SG+ E   N   +++ E  +N D 
Sbjct: 409 NERPTVPER-------TP--SMASRELLQLRERQTVSGLREGFHNGRENIVHENTSNTDN 468

Query: 415 DKNQSHTNAHTPDPHATN---VTNDKEKDNQNDNDNQQVVVGVHSTSIDNNNQIVGDFKE 474
           D + + TNA      A N   VT++    ++  ND+  +     S   + +     D  +
Sbjct: 469 DNSNTSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESNLANADRDWEEDTNQ 528

Query: 475 EQI-EEQEPAKPEPEREQE--QEEEGGSEVDPPSSEGIWQD-RPDLNLDSQDSIDGWEAE 534
            +I +E  P    P  EQ    + +G    D    E    D   + +  + D     EA 
Sbjct: 529 RRIWQENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREASGFADDEYRTQEAH 588

Query: 535 DQISEAAEESYGADYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIER 594
               E +      ++ GT     S+  R R   + RR + +    + N  + E+R+L+ R
Sbjct: 589 GVWHENSSRQSDGNWPGTR----SEALRSRRVVQLRRLNRFHPPEDDNVYSMELRELLSR 648

Query: 595 KTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYEDDDGAEEEEMWCFSEGHTQPKSSDNE 654
           ++VSN L S FRE +D+L+ +  ER+     +++                + Q    D+ 
Sbjct: 649 RSVSNLLHSGFRESLDQLIQSYAERRGHTHVDWD-------------LHANLQTAIPDSP 708

Query: 655 EEDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEM-GEDSNRGASTSSP 714
           E D +  +                                + DN++ G + ++   T   
Sbjct: 709 ERDTDHQVF------------------------------VRNDNQLNGINGSQLLPTPPA 768

Query: 715 QPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQL 774
            P QP +  +    S  R + H   IE E++ DLRG + +L Q MS++++ ++ CMDMQL
Sbjct: 769 PPPQPIWHQDLHHTSWSRHSMHRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEACMDMQL 828

Query: 775 MLQHSIKRNEVG-------------------GGRKSR--KPKCCICYNMQIDSLLYRCGH 822
            LQ S+ R EV                    G R S   K  CC+C +  ID+LLYRCGH
Sbjct: 829 ELQRSV-RQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDALLYRCGH 859

BLAST of Lsi01G000070 vs. TAIR 10
Match: AT4G20160.1 (LOCATED IN: chloroplast; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G30860.1); Has 64917 Blast hits to 39974 proteins in 1949 species: Archae - 279; Bacteria - 7425; Metazoa - 26604; Fungi - 5788; Plants - 3193; Viruses - 275; Other Eukaryotes - 21353 (source: NCBI BLink). )

HSP 1 Score: 127.1 bits (318), Expect = 6.6e-29
Identity = 144/473 (30.44%), Postives = 225/473 (47.57%), Query Frame = 0

Query: 349  LEDEQKRPQFVITPRENHRSSSIMHLRERFSGVGENSARSLIGEMLNNNDEDKNQSHTNA 408
            +ED+   PQ VI     H S      +ER S        SL      +  E + Q  T  
Sbjct: 746  IEDKNMNPQAVI-----HGSKE--RDKERNSSQNGEETLSLRNSEAKSTKEIERQEVTQE 805

Query: 409  HTPDPHATNVTNDKEKDNQNDNDNQQVVVGVHSTSID----NNNQIVGDFKEEQIE-EQE 468
                 H +    DKE+++    +    +    + S      N +Q V   +E      +E
Sbjct: 806  EKSVSHGSR-EKDKERNSLQYGEKMCFLRNSEAKSTKEIERNKSQEVSQGEESASHGSRE 865

Query: 469  PAKPEPEREQEQE---------EEGGSEVDPPSSEGIWQDRPDLNLD--SQDSIDGWEAE 528
             AK +   +Q+ E         ++G  E +   S+ + ++    N++  S + ++ W+  
Sbjct: 866  SAKEKNSSQQDDETSTHRNPNDKKGIKEPEDEESKKVEREETGENVEEASVEFVNDWDG- 925

Query: 529  DQISEAAEESYGADYVGTSYDWFSDISRPRSYWEDRRQSWYQQMLESNSANDEIRQLIER 588
            +++ E  EE YG DY     DW  DISRPRSYWED R+  Y ++L + S   +I  LIER
Sbjct: 926  NEMEEEEEEEYG-DYFNGDDDWIHDISRPRSYWEDLRKERYLEVLNTESEKKDICNLIER 985

Query: 589  KTVSNFLSSDFRERMDKLMVTRLE-------RQTQQEEEYEDDDGAEEEEMWCFSEGHTQ 648
            +TVSNFL+SD R+++D LM+TR++        Q ++ +EYE     EE E+ C +     
Sbjct: 986  RTVSNFLTSDLRQKIDNLMITRVQSHIGVPLNQIEEGDEYE-----EEWEVECSAR---- 1045

Query: 649  PKSSDNE-EEDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYQLDNEMGEDSNR 708
                DNE EE+ E++ + A     SD   QS+     A  S + SW+++ D ++ +D+  
Sbjct: 1046 -NEEDNETEEEPEKTNLEA----PSDVCSQSS-----ARSSTMMSWNFR-DQDIDKDNEP 1105

Query: 709  GASTSSPQPFQPQFSSNNQQRSSLRSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIK 768
              S S P+P  P               T+  + +M+ I DL+  MEQL +EM ELR ++K
Sbjct: 1106 TTSLSLPEPLVP---------------TNQSTQDMQTISDLKEQMEQLQREMLELRNTVK 1165

Query: 769  CCMDMQLMLQHSIKRNEVGGGRKSR----------KPKCCICYNMQIDSLLYR 788
             C+DMQL  Q S+ ++    G  +           K KCC+C  M +DSLLYR
Sbjct: 1166 SCIDMQLHFQKSVTQDLSRSGSSAEQRVDPKKDPLKRKCCVCSEMPVDSLLYR 1173

HSP 2 Score: 99.8 bits (247), Expect = 1.1e-20
Identity = 121/406 (29.80%), Postives = 184/406 (45.32%), Query Frame = 0

Query: 1   MASSQVEISSSSSPFGCVL---------RDHN-------RRREPNVTATHVARFRNNLKT 60
           MASS+VE+SS  + F CVL         RD+N            NV   HVA F  NL  
Sbjct: 1   MASSEVEMSSCQT-FNCVLSRPHERCTQRDNNTNNNKKKNNNNNNVRNIHVAAFEKNLNV 60

Query: 61  LVMDRLNDCMTITPNQNQNQNPNPILANFRVPKTNHNTTTNAVPR--RSDDSRQHTSTTN 120
            V D L +C     +   +           VP+ + N ++ +  R  ++++SRQ      
Sbjct: 61  FVRDHLENCSVSVDDVVDDSIK-------AVPECSSNKSSVSDHRLSKTEESRQ------ 120

Query: 121 DPQTQTNSTPTPTPIPIPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANATN 180
           D  + ++S  + +    P +G  +N  S+  ASSLVQIWE R      +   +   + T+
Sbjct: 121 DVPSSSSSLGSDSD---PNSGQPEN-PSRNAASSLVQIWEARTTQQPPSSNQSLIDSRTS 180

Query: 181 ATVCSGKQETET-EQEQEQEQEQACSVAAGDFEDERYDAGPGSEDGFADWHSSRTSSSSP 240
           +   +  + +E+ E  +E +  Q       + E+E  +  P                  P
Sbjct: 181 SMGSNALENSESLESVKESQMIQPIEECNNEEEEEEIECAP------------------P 240

Query: 241 PSSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSWVEDHNDHPNESSHPTLIPRDQV 300
           P       S   +RE VRV+DIIR+L+  +    ++      ND+  E            
Sbjct: 241 P-----LESGEKDREGVRVMDIIRKLSNDSETITNNDNGSSGNDNSKEV--------QTT 300

Query: 301 EPRCLSHILCSPRIRGRQAFADLLLQIERDRQRELDILVDRRAVSKFPQRGRIQSLLRLK 360
           E R    + CSPRIRGRQA ADLL+Q+ RDR+++L  L +R  VSKF  RGRIQS LR++
Sbjct: 301 EARSFPQVACSPRIRGRQALADLLVQMTRDREKDLACLRERHCVSKFTNRGRIQSTLRIR 357

Query: 361 ILKRGMALEDEQKRPQFVITPRENH--RSSSIMH-LRERFSGVGEN 385
             +R +A++   +          N   R S +MH LRE++    EN
Sbjct: 361 CYERCLAIQGRHRSKSTSAGSDSNRSSRGSGVMHLLREKYKANSEN 357