Lcy13g015680 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g015680
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionCytochrome p450 76c2
LocationChr13: 42475643 .. 42477567 (-)
RNA-Seq ExpressionLcy13g015680
SyntenyLcy13g015680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGTTCAGTTTATCATTAATATATATTTAAAAAATTTAAAATAACACAGTACAATGCACATGTATACCCCAGAGCCCTTTTTTTTCTTTTCTGCTGTTTTTTTTTAACCGTGGAGGCGAGAGATTTGAACCTTTGGCCTCTCGGTCAAGAGTACAAATTTTATGTCAGTTGTGCTGTATTTAGCTGATCTATTCACAAAATTTGTTTAAGAACACTGAAATCATGAAGAATAATTTGTTTTGAAACATCCTAAAGAACCATCAATTAAGAAGATGGCATCTCAGGAAGACTTTCTTCTTTGTGTCGTTGTTCTTCTTCTTACTTTCCTTATCATTCTTCTCAAATTCATCAGCTCTTCATCTTCAAAACAACCCCCAATTCCACCTGGTCCAAAACCATGGCCAGTTCTTGGAAACCTCCTTCATATTGGCAAAAATGCTCACATTTCAATTACTCGCTTTGCCGAAACCTATGGCCCTCTGATCTCACTCCGACTCGGAGCTCAACTCCTCGTCGTCGCTTCGTCTCCGGCCGCTTCCGCTGCCGTTCTCAAGACCCAAGATCGTCTTCTATCGGGTCGCTACATTTTTCATATGACTCCTGATGCAGCACAGCATCACAAACTCTCCTTCATTTTCTCTCCCGAGTGCGATGATCATTGGAAGAATTTGAGGTCCATTTGCAAGGCCAATTTTTTCTCAACCAAAGCAATTGAATCACAGGCCACTTTGAGAGCGAAAAAGATGACTGAAATGGTGGACTTCTTGCTGTCGAGGCAAGGCTCTGTGGTTGAGATTAGAGATTTTGTTTTTACAACTGCTTTCAACATTCTCTCTAACTTGATTTTCTCAAGGGATTTGTTTCACTTTGTTGATGATGGACTCAATAATGGAATCAAAGGATCTTTGGAGAAGCTGATGAATATGGGTTTGACTCCTAATTTAGCAGACTATTATCCCTTATTGAGAAATTTAGATATCCAAGGTCATCGAAAGAAAGTTACAATTTATAGGAAGGAAATCGAGTCGGCATGGGAAGTTCTTCTAAAGGAAAGAAGAAGAATCCACAGTGAAAATGCTTCTTATGCTGCATGTGACCTTTTGGATGTCTTTATTCAAAGTCAATTCACTGACGAGCAAATTAACTACTTAGTTGTTGTAAGCAAATAATCAAACCCTTCTTTCGTTCTTTCTTTTTTTTAACATGTATTTTTTTGTTAATTTTTATATCCATAACCTAATGGAAAATTCAATATCTAATTTTTTTTTTTTTTCGCGTGGTAGGAATTGTTCATGGCAGGTATAGATACAACTGCCATAACGGTTGAGTGGGCAATGACTGAATTGTTGAAGAACAAAGACATAATGAAAAAAGTGCAAGAGGAGATGAAACGAGAAATAAACCAAAATATGATCGATGAGTCAAACGTTTCTCAACTCCACTATTTGTGGAAATGCGTGAAAGAAACTCTAAGACTATACCCACCCGCACCGTTTCTTCTTCCACGTTTAGCTCCAATGGGTTGTGAGCTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTTAATGTTTGGGGGATTGGAAGAGACCCTTCCATATGGGAAGACTCTTTATCATTCAATCCTGAAAGATTTACGGCTTCTGATGTGGACTTCAAAGGTTATGATTATCGATACCTTCCATTTGGAGGAGGGAGAAGAACGTGTCCTGGAATTCCCATGGCGACCGTGCAAGTTCCATTAATTTTAGCCACGTTAATTCAGAACTTTGATTGGTCTCTTCCTAATGATGAGGATCTTTCAAAACTCGATTTAAATGGGATGTTTGGCGTAACGTTGCGGAAGGAAGTACCCCTCAAACTCATTCCCAAAAGAAGGCTTTGATACCTTCGGGTCTTTTTTATAATGTTCTCGTTTCC

mRNA sequence

CATGTTCAGTTTATCATTAATATATATTTAAAAAATTTAAAATAACACAGTACAATGCACATGTATACCCCAGAGCCCTTTTTTTTCTTTTCTGCTGTTTTTTTTTAACCGTGGAGGCGAGAGATTTGAACCTTTGGCCTCTCGGTCAAGAGTACAAATTTTATGTCAGTTGTGCTGTATTTAGCTGATCTATTCACAAAATTTGTTTAAGAACACTGAAATCATGAAGAATAATTTGTTTTGAAACATCCTAAAGAACCATCAATTAAGAAGATGGCATCTCAGGAAGACTTTCTTCTTTGTGTCGTTGTTCTTCTTCTTACTTTCCTTATCATTCTTCTCAAATTCATCAGCTCTTCATCTTCAAAACAACCCCCAATTCCACCTGGTCCAAAACCATGGCCAGTTCTTGGAAACCTCCTTCATATTGGCAAAAATGCTCACATTTCAATTACTCGCTTTGCCGAAACCTATGGCCCTCTGATCTCACTCCGACTCGGAGCTCAACTCCTCGTCGTCGCTTCGTCTCCGGCCGCTTCCGCTGCCGTTCTCAAGACCCAAGATCGTCTTCTATCGGGTCGCTACATTTTTCATATGACTCCTGATGCAGCACAGCATCACAAACTCTCCTTCATTTTCTCTCCCGAGTGCGATGATCATTGGAAGAATTTGAGGTCCATTTGCAAGGCCAATTTTTTCTCAACCAAAGCAATTGAATCACAGGCCACTTTGAGAGCGAAAAAGATGACTGAAATGGTGGACTTCTTGCTGTCGAGGCAAGGCTCTGTGGTTGAGATTAGAGATTTTGTTTTTACAACTGCTTTCAACATTCTCTCTAACTTGATTTTCTCAAGGGATTTGTTTCACTTTGTTGATGATGGACTCAATAATGGAATCAAAGGATCTTTGGAGAAGCTGATGAATATGGGTTTGACTCCTAATTTAGCAGACTATTATCCCTTATTGAGAAATTTAGATATCCAAGGTCATCGAAAGAAAGTTACAATTTATAGGAAGGAAATCGAGTCGGCATGGGAAGTTCTTCTAAAGGAAAGAAGAAGAATCCACAGTGAAAATGCTTCTTATGCTGCATGTGACCTTTTGGATGTCTTTATTCAAAGTCAATTCACTGACGAGCAAATTAACTACTTAGTTGTTGAATTGTTCATGGCAGGTATAGATACAACTGCCATAACGGTTGAGTGGGCAATGACTGAATTGTTGAAGAACAAAGACATAATGAAAAAAGTGCAAGAGGAGATGAAACGAGAAATAAACCAAAATATGATCGATGAGTCAAACGTTTCTCAACTCCACTATTTGTGGAAATGCGTGAAAGAAACTCTAAGACTATACCCACCCGCACCGTTTCTTCTTCCACGTTTAGCTCCAATGGGTTGTGAGCTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTTAATGTTTGGGGGATTGGAAGAGACCCTTCCATATGGGAAGACTCTTTATCATTCAATCCTGAAAGATTTACGGCTTCTGATGTGGACTTCAAAGGTTATGATTATCGATACCTTCCATTTGGAGGAGGGAGAAGAACGTGTCCTGGAATTCCCATGGCGACCGTGCAAGTTCCATTAATTTTAGCCACGTTAATTCAGAACTTTGATTGGTCTCTTCCTAATGATGAGGATCTTTCAAAACTCGATTTAAATGGGATGTTTGGCGTAACGTTGCGGAAGGAAGTACCCCTCAAACTCATTCCCAAAAGAAGGCTTTGATACCTTCGGGTCTTTTTTATAATGTTCTCGTTTCC

Coding sequence (CDS)

ATGGCATCTCAGGAAGACTTTCTTCTTTGTGTCGTTGTTCTTCTTCTTACTTTCCTTATCATTCTTCTCAAATTCATCAGCTCTTCATCTTCAAAACAACCCCCAATTCCACCTGGTCCAAAACCATGGCCAGTTCTTGGAAACCTCCTTCATATTGGCAAAAATGCTCACATTTCAATTACTCGCTTTGCCGAAACCTATGGCCCTCTGATCTCACTCCGACTCGGAGCTCAACTCCTCGTCGTCGCTTCGTCTCCGGCCGCTTCCGCTGCCGTTCTCAAGACCCAAGATCGTCTTCTATCGGGTCGCTACATTTTTCATATGACTCCTGATGCAGCACAGCATCACAAACTCTCCTTCATTTTCTCTCCCGAGTGCGATGATCATTGGAAGAATTTGAGGTCCATTTGCAAGGCCAATTTTTTCTCAACCAAAGCAATTGAATCACAGGCCACTTTGAGAGCGAAAAAGATGACTGAAATGGTGGACTTCTTGCTGTCGAGGCAAGGCTCTGTGGTTGAGATTAGAGATTTTGTTTTTACAACTGCTTTCAACATTCTCTCTAACTTGATTTTCTCAAGGGATTTGTTTCACTTTGTTGATGATGGACTCAATAATGGAATCAAAGGATCTTTGGAGAAGCTGATGAATATGGGTTTGACTCCTAATTTAGCAGACTATTATCCCTTATTGAGAAATTTAGATATCCAAGGTCATCGAAAGAAAGTTACAATTTATAGGAAGGAAATCGAGTCGGCATGGGAAGTTCTTCTAAAGGAAAGAAGAAGAATCCACAGTGAAAATGCTTCTTATGCTGCATGTGACCTTTTGGATGTCTTTATTCAAAGTCAATTCACTGACGAGCAAATTAACTACTTAGTTGTTGAATTGTTCATGGCAGGTATAGATACAACTGCCATAACGGTTGAGTGGGCAATGACTGAATTGTTGAAGAACAAAGACATAATGAAAAAAGTGCAAGAGGAGATGAAACGAGAAATAAACCAAAATATGATCGATGAGTCAAACGTTTCTCAACTCCACTATTTGTGGAAATGCGTGAAAGAAACTCTAAGACTATACCCACCCGCACCGTTTCTTCTTCCACGTTTAGCTCCAATGGGTTGTGAGCTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTTAATGTTTGGGGGATTGGAAGAGACCCTTCCATATGGGAAGACTCTTTATCATTCAATCCTGAAAGATTTACGGCTTCTGATGTGGACTTCAAAGGTTATGATTATCGATACCTTCCATTTGGAGGAGGGAGAAGAACGTGTCCTGGAATTCCCATGGCGACCGTGCAAGTTCCATTAATTTTAGCCACGTTAATTCAGAACTTTGATTGGTCTCTTCCTAATGATGAGGATCTTTCAAAACTCGATTTAAATGGGATGTTTGGCGTAACGTTGCGGAAGGAAGTACCCCTCAAACTCATTCCCAAAAGAAGGCTTTGA

Protein sequence

MASQEDFLLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLIPKRRL
Homology
[Close]
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.4e-119
Identity = 223/489 (45.60%), Postives = 321/489 (65.64%), Query Frame = 0 

Query: 8   LLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETY 67
           +L + ++  +FL+ L   +  S  K   +PPGP+P P++GNLL +G   H    + A+ Y
Sbjct: 3   VLSIAIVSFSFLLFLFFILRDSRPKN--LPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 62

Query: 68  GPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECD 127
           G L SL+LG+Q +VVASSPAA+A +LKT D++LSGRY+F  +    +H + S ++S EC+
Sbjct: 63  GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVF-QSFRVKEHVENSIVWS-ECN 122

Query: 128 DHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNIL 187
           D+WK LR +C+   F+ K IESQ+ +R  K  EMV FL  ++G VV+I + VF T  NI 
Sbjct: 123 DNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIF 182

Query: 188 SNLIFSRDLFHFVD-DGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIY 247
            NLIFS+D+F   D  G +  +K  L KL++MG + N ADY+P++  LD+ G R+ V   
Sbjct: 183 GNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEV 242

Query: 248 RKEIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTA 307
            ++I   W V+LKERR       S +  D +DV + +   D++IN L++ELF AG +T+A
Sbjct: 243 LQQIYDVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSA 302

Query: 308 ITVEWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPF 367
            T+EWA+TEL K   ++ K++ E+   +  N + ES++  L YL   VKETLRL+PP P 
Sbjct: 303 STIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPL 362

Query: 368 LLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDY 427
           LLPR A   C +M Y+IPK+  I VN W IGRDP  W+D L+F PERF +SDVD+KG D+
Sbjct: 363 LLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDF 422

Query: 428 RYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEV 487
             +PFGGGRR CPG+P+A+    LI+ATL+QNF+WSLP     S+L ++  FG+TL+K+ 
Sbjct: 423 ELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDP 482

Query: 488 PLKLIPKRR 496
           PL ++ K R
Sbjct: 483 PLLIVLKAR 484

[Close]
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 5.4e-116
Identity = 209/485 (43.09%), Postives = 304/485 (62.68%), Query Frame = 0 

Query: 13  VLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLIS 72
           V L+  +I  + ++    S    +PPGPKPWP++GNLL +G+  H      A+TYG + +
Sbjct: 5   VALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFT 64

Query: 73  LRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWKN 132
           L++G + +VVAS+ +A++ +LKT DR+LS RY+F       + H  + I   +C + WKN
Sbjct: 65  LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSF--RVKGHVENSIVWSDCTETWKN 124

Query: 133 LRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLIF 192
           LR +C+   F+ K IESQA +R KK  EMV++L+ +QG  V+I + +F T  NI  NLIF
Sbjct: 125 LRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIF 184

Query: 193 SRDLFHFVDDGLNNG-IKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIE 252
           S+++F   D    +   K  L +++ +G + N ADY+P+L   D+ G RK+V    K I 
Sbjct: 185 SQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 244

Query: 253 SAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEW 312
           + W  +L+ER+     +   +  D +DV + S   D QIN L++ELF AG +T+A T+EW
Sbjct: 245 AIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEW 304

Query: 313 AMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRL 372
           AMTEL KN  I  K++ E++  + +  + ES+   L YL   VKETLRL+PP P LLPR 
Sbjct: 305 AMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRR 364

Query: 373 APMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPF 432
           A   C ++ Y+IPKD  I VN WGIGRDP  W D L+F+PERF  S VDF+G D+  +PF
Sbjct: 365 ALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPF 424

Query: 433 GGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLI 492
           G GRR CPG+P+A   + L++AT +QN DW LPN   +  L +   FG+TL+KE PL ++
Sbjct: 425 GAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIV 484

Query: 493 PKRRL 497
           PK R+
Sbjct: 485 PKSRV 487

[Close]
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 9.2e-116
Identity = 210/487 (43.12%), Postives = 307/487 (63.04%), Query Frame = 0 

Query: 11  VVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPL 70
           V V L+  +I  + ++   SS    +PPGPKPWP++GNLL +G+  H      A+TYG +
Sbjct: 4   VTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDI 63

Query: 71  ISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHW 130
            +L++G + +VVAS+ +A++ +LKT DR+LS RY+F       + H  + I   +C + W
Sbjct: 64  FTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSF--RVKGHVENSIVWSDCTETW 123

Query: 131 KNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNL 190
           KNLR +C+   F+ K IESQA +R KK  EMV++L+ +QG  V+I + +F T  NI  NL
Sbjct: 124 KNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNL 183

Query: 191 IFSRDLFHF-VDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKE 250
           IFS+++F     +  ++  K  L +++ +G + N ADY+P+L   D+ G RK+V    K 
Sbjct: 184 IFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 243

Query: 251 IESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITV 310
           I + W  +L+ER+     +   +  D +DV + S   D QIN L++ELF AG +T+A T+
Sbjct: 244 IYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTI 303

Query: 311 EWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLP 370
           EWAMTEL KN  I  K++ E++  + +  + ES+   L YL   VKETLRL+PP P LLP
Sbjct: 304 EWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLP 363

Query: 371 RLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYL 430
           R A   C ++ Y+IPKD  I VN WGIGRDP  W D L+F+PERF  S VDF+G D+  +
Sbjct: 364 RRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLI 423

Query: 431 PFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLK 490
           PFG GRR CPG+P+A   + L++AT +QN DW LPN   +  L +   FG+TL+KE PL 
Sbjct: 424 PFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLF 483

Query: 491 LIPKRRL 497
           ++PK R+
Sbjct: 484 IVPKSRV 488

[Close]
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.3e-114
Identity = 211/486 (43.42%), Postives = 310/486 (63.79%), Query Frame = 0 

Query: 11  VVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPL 70
           V V++ TFL ++ +      S    +PPGPKPWP++GNLL +G+  H    + AETYG L
Sbjct: 3   VAVVITTFLYLIFR-----DSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDL 62

Query: 71  ISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHW 130
            SL+LG++ +VVAS+P A++ +LKT DR+LSGRY+F  +    +H + S ++S EC++ W
Sbjct: 63  FSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVF-QSFRVKEHVENSIVWS-ECNETW 122

Query: 131 KNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNL 190
           K LR +C+   F+ K IESQA +R  K  EMV++L    G+ V+I + VF T  NI  NL
Sbjct: 123 KKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNL 182

Query: 191 IFSRDLFHFVDDGLNN-GIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKE 250
           IFS+++F   D+   +  +K  L +++ +G + N ADY+P L   D+ G  K V    + 
Sbjct: 183 IFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQG 242

Query: 251 IESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITV 310
           I S W  +LKER+     N S    D +++ + S   D+QIN L++E+F AG +T+A T+
Sbjct: 243 IYSVWGAMLKERKIAKQHNNS-KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTI 302

Query: 311 EWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLP 370
           EWA++EL KN  +   ++ E+   + +  + ES++  + YL   VKETLRL+P  P LLP
Sbjct: 303 EWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLP 362

Query: 371 RLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYL 430
           R A   C+++ Y+IPK+  I VN WGIGRDP  W D L F+PERF  S +DFKG D+  +
Sbjct: 363 RRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELI 422

Query: 431 PFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLK 490
           PFG GRR CPG+P+AT  + LI+++L+QNFDW  P   D S+L +   FG+TL+KE PL 
Sbjct: 423 PFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLY 480

Query: 491 LIPKRR 496
           ++PK R
Sbjct: 483 IVPKTR 480

[Close]
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.9e-109
Identity = 202/487 (41.48%), Postives = 307/487 (63.04%), Query Frame = 0 

Query: 13  VLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNA----HISITRFAETYG 72
           + L + + ++L FI     K   +PPGP PWP++GNL  +  N     HI++   A T+G
Sbjct: 5   IALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHG 64

Query: 73  PLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDD 132
           P++ L LG Q  V+AS+  A+  +LKT DR+ S R+I  M+     H K S ++S +C D
Sbjct: 65  PIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS-DCTD 124

Query: 133 HWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILS 192
           +WK LR I +   FS K +++Q+ +R +K+ E++DFL S++G VV+I  FVF T  NIL 
Sbjct: 125 YWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILG 184

Query: 193 NLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRK 252
           N++FS+D+F + D+    GI+  + +++ +G  PN+A++YP L  LD+QG +KK      
Sbjct: 185 NVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFI 244

Query: 253 EIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAIT 312
            +   WE  +KER+   +E +     D+LDV + + F D QIN L +E F  G +T++ T
Sbjct: 245 RVMKMWEGTVKERKANRNEESK----DMLDVLLANDFNDAQINALFLETFGPGSETSSAT 304

Query: 313 VEWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLL 372
           +EW + EL+K+   M KV++E+   +  + I ES++ QL YL  C+KE +RL+P APFLL
Sbjct: 305 IEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLL 364

Query: 373 PRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRY 432
           PR A   CE+MGY+IPK++ + VN + IGRDP  W+D  +F PERF  SDVDF G  Y++
Sbjct: 365 PRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQF 424

Query: 433 LPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPL 492
           +PFG GRRTC G+P+AT  +PLI+ +L+ N+D+ LP       L +N M  +TL  +  L
Sbjct: 425 IPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSL 484

Query: 493 KLIPKRR 496
            ++PK R
Sbjct: 485 CVVPKAR 485

[Close]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match: A0A6J1E237 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia OX=3673 GN=LOC111025729 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 8.4e-205
Identity = 357/495 (72.12%), Postives = 418/495 (84.44%), Query Frame = 0 

Query: 4   QEDFLLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRF 63
           QE  L+  ++LLL  L I LK+  S SSKQ  +PPGPKPWP+LGNLL IG++AH S+TRF
Sbjct: 9   QEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRF 68

Query: 64  AETYGPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFS 123
           A+ YGPLISLRLG QLLVVASSP A+A VLKTQDRLLS RYIF    D A H + SF+FS
Sbjct: 69  AQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFS 128

Query: 124 PECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTA 183
           PEC+D W+ +RSIC+AN F+ KAI+SQA LR  KM EMV+FL+S+QGSVVEIRDFVFTT 
Sbjct: 129 PECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTV 188

Query: 184 FNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKV 243
           FNILSN IFSRDLF FV DG  NGIK SL+K++ +GLTPNLADYY LLRNLD+QG RKK 
Sbjct: 189 FNILSNFIFSRDLFDFVGDGF-NGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKA 248

Query: 244 TIYRKEIESAWEVLLKERRRIHSENA-SYAACDLLDVFIQSQFTDEQINYLVVELFMAGI 303
           TIYRK+I+SAW+ L+ ERR+IH +N+ ++ A D LDV IQSQF DEQIN+L+VE+F+AG 
Sbjct: 249 TIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGT 308

Query: 304 DTTAITVEWAMTELLKNKDIMKKVQEEMKREI-NQNMIDESNVSQLHYLWKCVKETLRLY 363
           DTT +TVEWAM E+LK +DIM KV+EEMKREI NQ+ +DES +SQLHYLWKCVKETLRL+
Sbjct: 309 DTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLH 368

Query: 364 PPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDF 423
           PPAPFLLPR+AP  CELMGYSIPKDTLIFVNVWGIGRDPS+WEDSLSFNPERFT  DVDF
Sbjct: 369 PPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDF 428

Query: 424 KGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVT 483
           KGYDYRYLPFGGGRR CPG+PMA+VQ+PLILATLI NF+ SLPN +D SKLDL+G  GVT
Sbjct: 429 KGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVT 488

Query: 484 LRKEVPLKLIPKRRL 497
           L+K+ PL+LI ++RL
Sbjct: 489 LQKKKPLQLILQKRL 502

[Close]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 7.9e-195
Identity = 338/484 (69.83%), Postives = 394/484 (81.40%), Query Frame = 0 

Query: 12  VVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLI 71
           ++LLL  + I+L+ I SSSSKQPP+PPGPKPWP+LGNLL IG+NAHIS+T+F+  YGPLI
Sbjct: 9   LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 72  SLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWK 131
           SL+LGAQ LVVASSPAA+AA+LKTQDRLLS RYIF MTPD   H   S +FSPEC D WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 132 NLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLI 191
           NLRSIC+A  F+ +AIESQA+LR KKM EMV+FL S+QGSVVEI++ VF T FN LSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 192 FSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIE 251
           FSRDL  FV DG  NGIKG  + ++++GL PNLAD+YPLLR  D+QG RKK  IY+ +++
Sbjct: 189 FSRDLVGFVGDGF-NGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 248

Query: 252 SAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEW 311
           S+WE+L+KERR IHS+  S AA D LDV IQS FTDEQI+YL+VE+  AG DTT  TVEW
Sbjct: 249 SSWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEW 308

Query: 312 AMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRL 371
           AM ELLKN+DIM +V+EE K+ IN   IDES +S+LHYLWKCVKETLRL+PPAPFLLPR 
Sbjct: 309 AMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 372 APMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPF 431
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIWED  SF PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428

Query: 432 GGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLI 491
           GGGRR CPG+PMA VQVPLILATLI NF+W LPNDED S LDLNG  GVTL+KE PLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 492 PKRR 496
            K R
Sbjct: 489 SKNR 489

[Close]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 8.7e-194
Identity = 335/484 (69.21%), Postives = 395/484 (81.61%), Query Frame = 0 

Query: 12  VVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLI 71
           ++LLL  +  +L+ I  SSSKQPP+PPGP PWP+LGNLL IG+NAHIS+T+F+  YGPLI
Sbjct: 9   LLLLLPLIFTILRHI-CSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 72  SLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWK 131
           SL+LGAQ LVVASSPAA+AA+LKTQDRLLS RYIF MTPD   H   S +FSPEC D WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 132 NLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLI 191
           NLRSIC+A  F+ +AIESQA+LR KKM +MV+FL S+QGSVVEI++ VF T FN LSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 192 FSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIE 251
           FSRDL  FV DG  NGIKGS + ++++GL PNLAD+YPLLR+ D+QG RKK  IY+ +++
Sbjct: 189 FSRDLVGFVGDGF-NGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVD 248

Query: 252 SAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEW 311
           S+WE+L+KERR IHS + S AA D LDV I+S FTDEQI+YL+VE+  AG DTT  TVEW
Sbjct: 249 SSWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEW 308

Query: 312 AMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRL 371
           AM ELLKN+DIM +V+EE K+ IN   IDES +S+LHYLWKCVKETLRL+PPAPFLLPR 
Sbjct: 309 AMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 372 APMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPF 431
           AP  CELMGYS+PKDT+IFVNVWGIGRDPSIWED  SF PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428

Query: 432 GGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLI 491
           GGGRR CPG+PMA VQVPLILATLI NF+W LPNDED S LDLNG  GVTL+KE PLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 492 PKRR 496
            K+R
Sbjct: 489 SKKR 489

[Close]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.6e-192
Identity = 336/484 (69.42%), Postives = 388/484 (80.17%), Query Frame = 0 

Query: 13  VLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLIS 72
           +LLL  +  +LK I SSSSKQPP PPGPKPWP+LGNLL IG+NAHIS+T+F+  YGPLIS
Sbjct: 9   LLLLPLIFAILKHI-SSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLIS 68

Query: 73  LRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWKN 132
           L+LGAQ LVVASSPAA+AA+LKTQDRLLS RYIF M PD A H   S +FSPEC D WKN
Sbjct: 69  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKN 128

Query: 133 LRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLIF 192
           LRSIC+A  F+ +AIESQA+LR KKM EMV+FL S+QGSVVEI++FVF T FN LSN IF
Sbjct: 129 LRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIF 188

Query: 193 SRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIES 252
           SRDL  FV DG  NGI+G  + +++  L PNLAD+YPLLR  D+QG RKK  IY+ +I+S
Sbjct: 189 SRDLVGFVGDGF-NGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 248

Query: 253 AWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEWA 312
           +WE+L+KERR IHS        D LDV IQS FTDEQINYL+VE+  AG DTT  TVEWA
Sbjct: 249 SWEILIKERRTIHSRGV-LVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWA 308

Query: 313 MTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRLA 372
           M ELLKN+DIM +V+EE K+ IN + IDES +S+LHYLWKCVKETLRL+PPAPFLLPR A
Sbjct: 309 MAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 368

Query: 373 PMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPFG 432
           P  CELMGYS+PK T+I VNVWGIGRDPSIWED  SF PERF   D D KG+DYR+LPFG
Sbjct: 369 PEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFG 428

Query: 433 GGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLIP 492
           GGRR CPG+PMA VQVPLILATLI NF+W LPNDED S LDL G  GVTL+KE PLKL+P
Sbjct: 429 GGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLP 488

Query: 493 KRRL 497
           KRR+
Sbjct: 489 KRRI 489

[Close]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.2e-192
Identity = 340/494 (68.83%), Postives = 400/494 (80.97%), Query Frame = 0 

Query: 8   LLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETY 67
           +L   +LLL  + I+LK I S SSK  P+PPGP PWP+LGNLL IG+NAHISIT+FA  Y
Sbjct: 8   ILLPSLLLLLPIFIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIY 67

Query: 68  GPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECD 127
           GPLISL+LGAQ L+VASSPAA+ AVLKTQDRLLS RYIF MTPD A H + S +FSPEC 
Sbjct: 68  GPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECG 127

Query: 128 DHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNIL 187
           D WKNLRS+CK N F+ KAIESQA LR KKM E+V FL S+QGSVVE++DFVFT+ FN L
Sbjct: 128 DQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTL 187

Query: 188 SNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYR 247
           SNLIFS DL  FV DG   GIKG  +K+M++GLTPNLAD+YPLLR  D+QG + K  IY+
Sbjct: 188 SNLIFSMDLIDFVGDGF-KGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYK 247

Query: 248 KEIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAI 307
            E++S W +L+KERR IH +  S A+ D LDV IQS F D+QIN L++EL  AG DTT  
Sbjct: 248 NEVDSLWGILIKERREIHCQQGS-ASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTT 307

Query: 308 TVEWAMTELLKNKDIMKKVQEEMKREINQNM-IDESNVSQLHYLWKCVKETLRLYPPAPF 367
           TVEWAM+E+LK+KDI+ KV+EEMK+   +N  IDESN+S+L YLW+CVKETLRL+PPAPF
Sbjct: 308 TVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPF 367

Query: 368 LLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTA----SDVDFK 427
           LLPRLAPM CE+MGYSIPKD+++FVNVWGIGRDPS+WEDS +FNP+RF       +VDFK
Sbjct: 368 LLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFK 427

Query: 428 GYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTL 487
           GYDYRYLPFGGGRR CPG+PMA VQVPLILATLI NF+WSLPNDEDLSKLDLNG  GVTL
Sbjct: 428 GYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTL 487

Query: 488 RKEVPLKLIPKRRL 497
           +K   LKLIPKRR+
Sbjct: 488 QKYETLKLIPKRRV 498

[Close]
BLAST of Lcy13g015680 vs. NCBI nr
Match: XP_022159319.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia])

HSP 1 Score: 723.0 bits (1865), Expect = 1.7e-204
Identity = 357/495 (72.12%), Postives = 418/495 (84.44%), Query Frame = 0 

Query: 4   QEDFLLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRF 63
           QE  L+  ++LLL  L I LK+  S SSKQ  +PPGPKPWP+LGNLL IG++AH S+TRF
Sbjct: 9   QEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRF 68

Query: 64  AETYGPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFS 123
           A+ YGPLISLRLG QLLVVASSP A+A VLKTQDRLLS RYIF    D A H + SF+FS
Sbjct: 69  AQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFS 128

Query: 124 PECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTA 183
           PEC+D W+ +RSIC+AN F+ KAI+SQA LR  KM EMV+FL+S+QGSVVEIRDFVFTT 
Sbjct: 129 PECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTV 188

Query: 184 FNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKV 243
           FNILSN IFSRDLF FV DG  NGIK SL+K++ +GLTPNLADYY LLRNLD+QG RKK 
Sbjct: 189 FNILSNFIFSRDLFDFVGDGF-NGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKA 248

Query: 244 TIYRKEIESAWEVLLKERRRIHSENA-SYAACDLLDVFIQSQFTDEQINYLVVELFMAGI 303
           TIYRK+I+SAW+ L+ ERR+IH +N+ ++ A D LDV IQSQF DEQIN+L+VE+F+AG 
Sbjct: 249 TIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGT 308

Query: 304 DTTAITVEWAMTELLKNKDIMKKVQEEMKREI-NQNMIDESNVSQLHYLWKCVKETLRLY 363
           DTT +TVEWAM E+LK +DIM KV+EEMKREI NQ+ +DES +SQLHYLWKCVKETLRL+
Sbjct: 309 DTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLH 368

Query: 364 PPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDF 423
           PPAPFLLPR+AP  CELMGYSIPKDTLIFVNVWGIGRDPS+WEDSLSFNPERFT  DVDF
Sbjct: 369 PPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDF 428

Query: 424 KGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVT 483
           KGYDYRYLPFGGGRR CPG+PMA+VQ+PLILATLI NF+ SLPN +D SKLDL+G  GVT
Sbjct: 429 KGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVT 488

Query: 484 LRKEVPLKLIPKRRL 497
           L+K+ PL+LI ++RL
Sbjct: 489 LQKKKPLQLILQKRL 502

[Close]
BLAST of Lcy13g015680 vs. NCBI nr
Match: XP_038892964.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])

HSP 1 Score: 703.7 bits (1815), Expect = 1.1e-198
Identity = 348/497 (70.02%), Postives = 412/497 (82.90%), Query Frame = 0 

Query: 1   MASQEDFLLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISI 60
           +ASQEDF+LC V+LLL  L ++ K IS  SSK+PPIPPGPKPWP+LGNLL IGKNAHISI
Sbjct: 9   VASQEDFVLC-VILLLPLLFVVFKLISFPSSKKPPIPPGPKPWPILGNLLQIGKNAHISI 68

Query: 61  TRFAETYGPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSF 120
           T FA+TYGPLISLRLG +LLVVASSPAA+AAVLKTQDRLLS RY+FHMTPD AQHH+ S 
Sbjct: 69  THFAQTYGPLISLRLGGRLLVVASSPAAAAAVLKTQDRLLSNRYVFHMTPDLAQHHECSL 128

Query: 121 IFSPECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVF 180
           +FSPEC+DHW++LRSICKAN F+ KAI+SQA+LR +KM EM++FLLS+QGSVVEIRDFV 
Sbjct: 129 VFSPECNDHWRSLRSICKANLFTAKAIKSQASLRRRKMNEMIEFLLSKQGSVVEIRDFVL 188

Query: 181 TTAFNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHR 240
           TT FN LSN IFSRDL  F  +G  NG+K S   L N+GL PNL D+YP LRNLD+QG R
Sbjct: 189 TTVFNALSNFIFSRDLIDFDGEGF-NGLKQSFNNLKNLGLAPNLGDFYPFLRNLDVQGLR 248

Query: 241 KKVTIYRKEIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMA 300
            K +IY K+I S  ++++ +RR++ S + S A  D LDV IQSQFT++ INYLVVEL +A
Sbjct: 249 NKCSIYSKQINSTLDIIINQRRKLASTDPS-APQDFLDVMIQSQFTNDHINYLVVELLLA 308

Query: 301 GIDTTAITVEWAMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRL 360
           G DT+ ITVEW M ELLKNK+I++KV+EE+  E+ QN IDES + +L YLWKCVKETLRL
Sbjct: 309 GTDTSTITVEWTMAELLKNKNIIEKVREEVNTEMKQNSIDESEIYKLKYLWKCVKETLRL 368

Query: 361 YPPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASD-V 420
           +PPAPFLLPR+AP+ CELMGYSIPK++LIFVNVWGIGRDPSIWE+SLSFNP RF+  D V
Sbjct: 369 HPPAPFLLPRIAPIDCELMGYSIPKESLIFVNVWGIGRDPSIWEESLSFNPMRFSDCDHV 428

Query: 421 DFKGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPN-DEDLSKLDLNGMF 480
           D KG D+ YLPFGGGRR CPG PMATV +PLILA LI NF+WSLPN DED SK+D+ GM 
Sbjct: 429 DLKGNDFGYLPFGGGRRICPGFPMATVLLPLILANLIHNFEWSLPNGDEDFSKMDMEGML 488

Query: 481 GVTLRKEVPLKLIPKRR 496
           GVTL K+ PLKLIPK+R
Sbjct: 489 GVTLLKKQPLKLIPKKR 502

[Close]
BLAST of Lcy13g015680 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 689.9 bits (1779), Expect = 1.6e-194
Identity = 338/484 (69.83%), Postives = 394/484 (81.40%), Query Frame = 0 

Query: 12  VVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLI 71
           ++LLL  + I+L+ I SSSSKQPP+PPGPKPWP+LGNLL IG+NAHIS+T+F+  YGPLI
Sbjct: 9   LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 72  SLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWK 131
           SL+LGAQ LVVASSPAA+AA+LKTQDRLLS RYIF MTPD   H   S +FSPEC D WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 132 NLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLI 191
           NLRSIC+A  F+ +AIESQA+LR KKM EMV+FL S+QGSVVEI++ VF T FN LSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 192 FSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIE 251
           FSRDL  FV DG  NGIKG  + ++++GL PNLAD+YPLLR  D+QG RKK  IY+ +++
Sbjct: 189 FSRDLVGFVGDGF-NGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 248

Query: 252 SAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEW 311
           S+WE+L+KERR IHS+  S AA D LDV IQS FTDEQI+YL+VE+  AG DTT  TVEW
Sbjct: 249 SSWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEW 308

Query: 312 AMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRL 371
           AM ELLKN+DIM +V+EE K+ IN   IDES +S+LHYLWKCVKETLRL+PPAPFLLPR 
Sbjct: 309 AMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 372 APMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPF 431
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIWED  SF PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428

Query: 432 GGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLI 491
           GGGRR CPG+PMA VQVPLILATLI NF+W LPNDED S LDLNG  GVTL+KE PLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 492 PKRR 496
            K R
Sbjct: 489 SKNR 489

[Close]
BLAST of Lcy13g015680 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-193
Identity = 333/483 (68.94%), Postives = 398/483 (82.40%), Query Frame = 0 

Query: 13  VLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLIS 72
           +LLL  +  +LK I SSSSKQPP+PPGP PWP+LGNLL IG+NAHIS+T+F+  YGPLIS
Sbjct: 9   LLLLPLIFTVLKHI-SSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 68

Query: 73  LRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWKN 132
           L+LGAQ LVVASSPAA+AA+LKTQDRLLS RYI  MTPD A H   S +FSPEC D WKN
Sbjct: 69  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECGDRWKN 128

Query: 133 LRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLIF 192
           LRSIC+A  F+ +AIESQA+LR KKM +MV+FL S+QGSVVEI++FVF T FN LSNLIF
Sbjct: 129 LRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITVFNTLSNLIF 188

Query: 193 SRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIES 252
           SRDL  FV DG  NGIKG  + ++++GL PNLAD+YPLLR+ D+QG RKK  IY+ +I+S
Sbjct: 189 SRDLVDFVGDGF-NGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQIDS 248

Query: 253 AWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEWA 312
           + E+L+KERR IHS   S AA D LD+ I+S FT+EQI+YL++E+ +AG DTT  TVEWA
Sbjct: 249 SSEILIKERRTIHSRGVS-AASDFLDILIESGFTNEQISYLILEVLLAGTDTTTTTVEWA 308

Query: 313 MTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRLA 372
           M EL+KN+DIM +V+EE K+ +N   IDES +S+LHYLWKCVKET+RL+PPAPFLLPR A
Sbjct: 309 MAELMKNRDIMNRVREETKKALNGGAIDESKLSELHYLWKCVKETVRLHPPAPFLLPRYA 368

Query: 373 PMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPFG 432
           P  CELMGYS+PK T+IFVNVWGIGRDPSIWED  SF PERF +SD D KG+DYR+LPFG
Sbjct: 369 PEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHDYRFLPFG 428

Query: 433 GGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLIP 492
           GGRRTCPG+PMA VQVPLILATLI NF+W LPNDED S LDLNG  G+TL+KE PLKL+P
Sbjct: 429 GGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKEEPLKLVP 488

Query: 493 KRR 496
           K+R
Sbjct: 489 KKR 488

[Close]
BLAST of Lcy13g015680 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 686.4 bits (1770), Expect = 1.8e-193
Identity = 335/484 (69.21%), Postives = 395/484 (81.61%), Query Frame = 0 

Query: 12  VVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLI 71
           ++LLL  +  +L+ I  SSSKQPP+PPGP PWP+LGNLL IG+NAHIS+T+F+  YGPLI
Sbjct: 9   LLLLLPLIFTILRHI-CSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 72  SLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDDHWK 131
           SL+LGAQ LVVASSPAA+AA+LKTQDRLLS RYIF MTPD   H   S +FSPEC D WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 132 NLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLSRQGSVVEIRDFVFTTAFNILSNLI 191
           NLRSIC+A  F+ +AIESQA+LR KKM +MV+FL S+QGSVVEI++ VF T FN LSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 192 FSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIE 251
           FSRDL  FV DG  NGIKGS + ++++GL PNLAD+YPLLR+ D+QG RKK  IY+ +++
Sbjct: 189 FSRDLVGFVGDGF-NGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVD 248

Query: 252 SAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDEQINYLVVELFMAGIDTTAITVEW 311
           S+WE+L+KERR IHS + S AA D LDV I+S FTDEQI+YL+VE+  AG DTT  TVEW
Sbjct: 249 SSWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEW 308

Query: 312 AMTELLKNKDIMKKVQEEMKREINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRL 371
           AM ELLKN+DIM +V+EE K+ IN   IDES +S+LHYLWKCVKETLRL+PPAPFLLPR 
Sbjct: 309 AMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 372 APMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFKGYDYRYLPF 431
           AP  CELMGYS+PKDT+IFVNVWGIGRDPSIWED  SF PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428

Query: 432 GGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLKLI 491
           GGGRR CPG+PMA VQVPLILATLI NF+W LPNDED S LDLNG  GVTL+KE PLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 492 PKRR 496
            K+R
Sbjct: 489 SKKR 489

[Close]
BLAST of Lcy13g015680 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 347.4 bits (890), Expect = 1.9e-95
Identity = 196/505 (38.81%), Postives = 292/505 (57.82%), Query Frame = 0 

Query: 8   LLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETY 67
           L C V  L  F+I        SS K  P PPGP   P++GN+  +G+N H S    ++TY
Sbjct: 12  LFCFV--LSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTY 71

Query: 68  GPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPD----AAQHHKLSFIFS 127
           GP++SL+ G+   VV +SP A+  VL+T D++LS R     TP     +  H K+S ++ 
Sbjct: 72  GPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWL 131

Query: 128 PECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFL--LSRQGSVVEIRDFVFT 187
           P     W+ LR +     FS + IE+  TLR  K+ E+V F+   S +   V+I    F 
Sbjct: 132 PPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFI 191

Query: 188 TAFNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRK 247
           TA NI+SN++FS DL ++ D   +   + ++  +M     P+ A+++P L  LD+QG+RK
Sbjct: 192 TALNIISNILFSVDLGNY-DSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRK 251

Query: 248 KVTIYRKEIESAW----EVLLKERRRIHSENASYAACDLLDVFI------QSQFTDEQIN 307
            +    + +   +    +  L E+    + +      D +DV +      +++     I 
Sbjct: 252 TLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIV 311

Query: 308 YLVVELFMAGIDTTAITVEWAMTELLKNKDIMKKVQEEMKREINQ-NMIDESNVSQLHYL 367
           +L+++LF AG DT + TVEWAM ELL+N + M K Q E+   I Q  +++ES++S L YL
Sbjct: 312 HLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYL 371

Query: 368 WKCVKETLRLYPPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFN 427
              VKET RL+P AP L+PR A    E++G+ +PKDT +FVNVW IGRDP++WE+S  F 
Sbjct: 372 QAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFK 431

Query: 428 PERFTASDVDFKGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLS 487
           PERF   D+D +G DY   PFG GRR CPG+P+A   VPL+LA+L+ +FDW LPN     
Sbjct: 432 PERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSE 491

Query: 488 KLDLNGMFGVTLRKEVPLKLIPKRR 496
            LD++  FG+TL K  PL  +P ++
Sbjct: 492 DLDMDETFGLTLHKTNPLHAVPVKK 508

[Close]
BLAST of Lcy13g015680 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 339.3 bits (869), Expect = 5.0e-93
Identity = 188/493 (38.13%), Postives = 285/493 (57.81%), Query Frame = 0 

Query: 18  FLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITRFAETYGPLISLRLGA 77
           FLI        SS +   +PPGP   P++GN+  +GKN H S    A+ YGP++SL+ G 
Sbjct: 20  FLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGC 79

Query: 78  QLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQ---HHKLSFIFSPECDDHWKNLR 137
              VV +SP A+  VL+T D++LSGR     + D+ +   H ++S I+ P     W+ LR
Sbjct: 80  LNSVVITSPEAAREVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLPPSSARWRMLR 139

Query: 138 SICKANFFSTKAIESQATLRAKKMTEMVDFL--LSRQGSVVEIRDFVFTTAFNILSNLIF 197
            +     FS +  E+   LR KK+ E+V F+   S +   V+I    +TT  NI+SN++F
Sbjct: 140 KLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILF 199

Query: 198 SRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIES 257
           S DL  + D   +N  + ++   M     P+ A+Y+P +  LD+QG+RK +    + +  
Sbjct: 200 SVDLGSY-DSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFR 259

Query: 258 AWEVLLKER---RRIHSENASYAACDLLDVFI------QSQFTDEQINYLVVELFMAGID 317
            +   +  +   + + + +   +  D LD  +      +++  +  I +L++++F AG D
Sbjct: 260 VFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTD 319

Query: 318 TTAITVEWAMTELLKNKDIMKKVQEEMKREINQN-MIDESNVSQLHYLWKCVKETLRLYP 377
           T++ T+EWAM ELL+N   M K Q EM R + QN ++ ES++S L YL   VKET RL+P
Sbjct: 320 TSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHP 379

Query: 378 PAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASDVDFK 437
            AP L+PR A    E++G+ +PKDT + VNVW IGRDPS+WE+   F PERF   D+D K
Sbjct: 380 AAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVK 439

Query: 438 GYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTL 496
           G DY   PFGGGRR CPG+P+A   V L+LA+L+ +FDW LPN      LD++  FG+TL
Sbjct: 440 GRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITL 499

[Close]
BLAST of Lcy13g015680 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-92
Identity = 199/509 (39.10%), Postives = 296/509 (58.15%), Query Frame = 0 

Query: 14  LLLTFLIILLKFISSSSSKQPPI-----PPGPKPWPVLGNLLHIGKNAHISITRFAETYG 73
           L+  F++  L F +++ S++ P      PPGP   P++GN+  +GKN H S T  ++TYG
Sbjct: 11  LIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYG 70

Query: 74  PLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIFSPECDD 133
           P++SL+LG    VV +S  A   VLKT D++LSGRYI   T  +  HH+ S  +      
Sbjct: 71  PVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEAT-KSNNHHEFSVGWIHPSSS 130

Query: 134 HWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFLLS--RQGSVVEIRDFVFTTAFNI 193
            ++ LR +     FS + I++   LR KK+ E+V+FL     +   V+I    F TA NI
Sbjct: 131 RFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNI 190

Query: 194 LSNLIFSRDLFHFVDDGLNNG-----IKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHRK 253
           +SN++FS +L  +  D  N+      + G  E + N    P+LA+++P +R LD+QG+ K
Sbjct: 191 ISNILFSVNLGSY--DSKNSSAFQEMVIGYQESIGN----PDLANFFPFMRFLDLQGNSK 250

Query: 254 K--------VTIYRKEIESAWEVLLKERRRIHSENASYAACDLLDVFIQSQFTDE----- 313
           K        + ++R+  ++   ++ K  R +  + +S    D LDV I  Q  DE     
Sbjct: 251 KMRESSGRLLQVFREFYDA--RIVEKSSRSVEKDVSSK---DFLDVLIDLQQGDETEINI 310

Query: 314 -QINYLVVELFMAGIDTTAITVEWAMTELLKNKDIMKKVQEEMKREINQN-MIDESNVSQ 373
            +I +L++++F+AG DT + TVEWAM ELL N   M KVQ+E+   I QN    ES++S+
Sbjct: 311 DEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISK 370

Query: 374 LHYLWKCVKETLRLYPPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDS 433
           L YL   VKET RL+P APFLL R A    E++G+++ KD+ + VNVW IGRDP +WE+ 
Sbjct: 371 LPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENP 430

Query: 434 LSFNPERFTASDVDFKGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPND 493
             F PERF   ++D KG DY   PFG GRR CPG+P+A   V L+LA+L+  F+W LPN 
Sbjct: 431 THFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNG 490

Query: 494 EDLSKLDLNGMFGVTLRKEVPLKLIPKRR 496
                LD+   FG+T+ K  PL  +P ++
Sbjct: 491 VGSEDLDMEETFGLTVHKTNPLLAVPLKK 507

[Close]
BLAST of Lcy13g015680 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 332.0 bits (850), Expect = 8.1e-91
Identity = 185/504 (36.71%), Postives = 285/504 (56.55%), Query Frame = 0 

Query: 3   SQEDFLLCVVVLLLTFLIILLKFISSSSSKQPPIPPGPKPWPVLGNLLHIGKNAHISITR 62
           S +  LL    +L  FLI         S     +PPGP   P++GN+  +GK+ H S   
Sbjct: 5   SGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAE 64

Query: 63  FAETYGPLISLRLGAQLLVVASSPAASAAVLKTQDRLLSGRYIFHMTPDAAQHHKLSFIF 122
            ++TYGP++SL+LG+   VV +SP A+  VL+T D++LS R   +    +  H   S ++
Sbjct: 65  LSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAV-RSINHQDASLVW 124

Query: 123 SPECDDHWKNLRSICKANFFSTKAIESQATLRAKKMTEMVDFL--LSRQGSVVEIRDFVF 182
            P     W+ LR +      S + IE+   LR  K+ E+V F+   S +   V+I    F
Sbjct: 125 LPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAF 184

Query: 183 TTAFNILSNLIFSRDLFHFVDDGLNNGIKGSLEKLMNMGLTPNLADYYPLLRNLDIQGHR 242
            T  NI+SN++FS DL  +      NG++ ++  +M+   TP+ A+Y+P LR LD+QG+ 
Sbjct: 185 ITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNV 244

Query: 243 KKVTIYRKEIESAWEVLLKER---RRIHSENASYAACDLLDVFI-----QSQFTDEQINY 302
           K   +  + +   +   +  +   +   +     +  D +D  +     +S+ +   I +
Sbjct: 245 KTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEH 304

Query: 303 LVVELFMAGIDTTAITVEWAMTELLKNKDIMKKVQEEMKREINQN-MIDESNVSQLHYLW 362
           L++++F AG DT++ T+EWAMTELLKN   M K Q E+   I QN +++ES++S+L YL 
Sbjct: 305 LLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQ 364

Query: 363 KCVKETLRLYPPAPFLLPRLAPMGCELMGYSIPKDTLIFVNVWGIGRDPSIWEDSLSFNP 422
             VKET RL+ P P L+PR A    E++G+ + KDT + VNVW IGRDPS+W++   F P
Sbjct: 365 AVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEP 424

Query: 423 ERFTASDVDFKGYDYRYLPFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSK 482
           ERF   D+D +G DY   PFG GRR CPG+P+A   V L+LA+L+ +FDW LP       
Sbjct: 425 ERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSED 484

Query: 483 LDLNGMFGVTLRKEVPLKLIPKRR 496
           LD++  FG+TL K  PL  +P ++
Sbjct: 485 LDMDETFGLTLHKTNPLHAVPVKK 507

[Close]
BLAST of Lcy13g015680 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 320.9 bits (821), Expect = 1.9e-87
Identity = 177/483 (36.65%), Postives = 281/483 (58.18%), Query Frame = 0 

Query: 36  IPPGPKPWPVLGNLLHI-GKNAHISITRFAETYGPLISLRLGAQLLVVASSPAASAAVLK 95
           +PPGP PWPV+GN+  + G   H S+T+ +  +GP+++LR+G+ L VV SS   +  + K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 96  TQDRLLSGRYIFHMTPDAAQHHKLS--FIFSPECDDHWKNLRSICKANFFSTKAIESQAT 155
             D  L+GR I+    +A +  K S   + + +   +W+ LR +C   FF T+ +++ + 
Sbjct: 96  KHDAALAGRKIY----EAMKGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSD 155

Query: 156 LRAKKMTEMVDFLL--SRQGS-VVEIRDFVFTTAFNILSNLIFSRDLFHFVDDGLNNGIK 215
           +R++ + +M+ F+    + G+  +++  + F  AFN++ NL+FSRDL    D    +   
Sbjct: 156 VRSRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD-PDSKRGSEFF 215

Query: 216 GSLEKLMNMGLTPNLADYYPLLRNLDIQGHRKKVTIYRKEIESAWEV---LLKERRRIHS 275
               K+M     PN+AD++PLLR LD QG R+K   +   +E A+E+    ++ER  +  
Sbjct: 216 YHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH---VEKAFEIAGEFIRERTEVRE 275

Query: 276 -ENASYAACDLLDVFIQ-----------SQFTDEQINYLVVELFMAGIDTTAITVEWAMT 335
            E +     D LDV ++           S F+   IN +V E+F AG DTT  T+EWA+ 
Sbjct: 276 REKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALA 335

Query: 336 ELLKNKDIMKKVQEEMKR--EINQNMIDESNVSQLHYLWKCVKETLRLYPPAPFLLPRLA 395
           ELL N   + K+Q E++   + +   + E ++  L YL   + ETLRL+PP PFL+P  A
Sbjct: 336 ELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKA 395

Query: 396 PMGCELMG-YSIPKDTLIFVNVWGIGRDPSIWEDSLSFNPERFTASD--VDFKGYDYRYL 455
              C +   Y+IPK+T + VNVW IGRDP  W D + F PERF +     DFKG DY +L
Sbjct: 396 MSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFL 455

Query: 456 PFGGGRRTCPGIPMATVQVPLILATLIQNFDWSLPNDEDLSKLDLNGMFGVTLRKEVPLK 493
           PFG GRR CP +P+A+  +PL + +++++FDW+L N  +  ++D+    G+TL+K VPL+
Sbjct: 456 PFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLE 510

The following BLAST results are available for this feature:
BLAST of Lcy13g015680 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FXW41.4e-11945.60Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O648995.4e-11643.09(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
O649009.2e-11643.12(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
Q9SP061.3e-11443.42(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
A8CDR54.9e-10941.48Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
BLAST of Lcy13g015680 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1E2378.4e-20572.12probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia ... [more]
A0A6J1ELA67.9e-19569.83probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1I4V78.7e-19469.21probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A6J1ER521.6e-19269.42probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A5A7UIU32.2e-19268.83Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
BLAST of Lcy13g015680 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022159319.11.7e-20472.12probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia][more]
XP_038892964.11.1e-19870.02probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida][more]
XP_022928791.11.6e-19469.83probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
XP_023551257.11.1e-19368.94probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ... [more]
XP_022970204.11.8e-19369.21probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima][more]
BLAST of Lcy13g015680 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45570.11.9e-9538.81cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45550.15.0e-9338.13cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT1G33720.11.9e-9239.10cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT2G45560.18.1e-9136.71cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT3G52970.11.9e-8736.65cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 313..333
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 10..475
e-value: 9.5E-18
score: 62.2
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 12..493
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 12..493
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 428..438
score: 54.17
coord: 392..416
score: 34.12
coord: 309..335
score: 30.36
coord: 351..369
score: 32.27
coord: 289..306
score: 35.35
coord: 438..461
score: 34.54
coord: 64..83
score: 34.44
IPR001128Cytochrome P450PRINTSPR00385P450coord: 300..317
score: 38.71
coord: 429..438
score: 59.55
coord: 352..363
score: 41.78
coord: 438..449
score: 28.74
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..478
e-value: 8.5E-98
score: 328.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 21..493
e-value: 3.4E-111
score: 374.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 37..495
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 431..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g015680.1Lcy13g015680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen