Lcy13g014210 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g014210
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPlant protein of unknown function (DUF247)
LocationChr13: 30328961 .. 30330337 (+)
RNA-Seq ExpressionLcy13g014210
SyntenyLcy13g014210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTGCGGTCATGTTGAAGCATATCACAATATTGATGAGGTTAAACGACAATCTCGGGATGAGATCACGGTATTCATCGAAGGAAAGCTTCAAGGAGTGCCTCGCACCGTTCCAACATATAGCCTCTATCGGGTTCCCAAACGGCTACTTGACATTAACCCTATAGCTTATGTGCCTCGAGTCATTTCAATTGGTCCTTTTCACCATCATCAACAGATTTTGAAGGACACAGAAGAGCTCAAGTTTCGATTTTTAAGTAGCTATCAACGTCGCGTAAATAAGAGCATTCAGGACATTGTGGAAAAGACTCGAAGTTGGGAGACAATAGCCCGTAAATGCTACTCAGAGCCCATAAACATGAACATTGATGACTTTGTGGGAATGATGGTTTTAGATGGTTGTTTCATAGTGGAGTTCATGATAATAAATTACGGTGACAATTTTCCCAAAAATGAAATGACGGTAAATTCTCCCTTGTATGAACCTATAAGATTTGATATATGTCGTGATTTGACAATTCTTGAGAACCAACTTCCTTTCTTTGTTCTACAAGGACTGTTTGACCTAGTTCCACTCCCTTCTAATACTACTATCTCCTTTCAACATGTTGTACACACATTTTTCAATAACAATATGTTGCAAGGTTGCCATAAGGCCCCTTATGATATGGCCAATTTTCAAAAGCCACGGCACTTGGTGGATTTCTTAAGGTTGTACTTTGTTCCCGAGAAGAGTGAAAATTGTCCACGCGATAGGTGCCTTCCCCCAAATGCAACCGCTCTTCGTGAGGCTGGTGTCGTCTTCAAGAAAGCAAAACGAGAGGAAAGACACATTATGGACATAAGGTTCAAAGATGGGGTCCTAGAAATTCCATCTTTCGGAATTGAAGATGATTTTGAAACATATGTACGAAACTTAATGGCATTTGAGCAGTTTTTGGTGGAGAATAATGAGGTGAAAAGTGAAGATCAGTGTCACAAGGAGCTTGATGAGAAGATTAGCAAAGAGAGCATTAAAAGGTATGCAACGGATTATTTTGAGTTCCTAGATGAGTTGATATCATCAGAGAAAGATGTGAGTTTACTTGTGAAGGAAGGAATCATAATCAATAGTATTGGTGGCAGTGATGAAGAAATTTCAAAATTGTTTAATGACCTTTGCAAGTATATCACCGTAGATGAATTTAACCACTTCTCCAATATTAGCGAAGATCTAAAAAAGCACTGTGAAAAACGATGGAACAGGTGGATGGCTTCATTGAGACACAACTATTTTAATACGCCATGGGCTTTTATTTCCTTCTTAGCTGCAACCTTCATTATTTTGCTCACTCTCCTACAAACCATATTCTCTGGTGTATCCATTTCCAAGTAA

mRNA sequence

ATGGATTGCGGTCATGTTGAAGCATATCACAATATTGATGAGGTTAAACGACAATCTCGGGATGAGATCACGGTATTCATCGAAGGAAAGCTTCAAGGAGTGCCTCGCACCGTTCCAACATATAGCCTCTATCGGGTTCCCAAACGGCTACTTGACATTAACCCTATAGCTTATGTGCCTCGAGTCATTTCAATTGGTCCTTTTCACCATCATCAACAGATTTTGAAGGACACAGAAGAGCTCAAGTTTCGATTTTTAAGTAGCTATCAACGTCGCGTAAATAAGAGCATTCAGGACATTGTGGAAAAGACTCGAAGTTGGGAGACAATAGCCCGTAAATGCTACTCAGAGCCCATAAACATGAACATTGATGACTTTGTGGGAATGATGGTTTTAGATGGTTGTTTCATAGTGGAGTTCATGATAATAAATTACGGTGACAATTTTCCCAAAAATGAAATGACGGTAAATTCTCCCTTGTATGAACCTATAAGATTTGATATATGTCGTGATTTGACAATTCTTGAGAACCAACTTCCTTTCTTTGTTCTACAAGGACTGTTTGACCTAGTTCCACTCCCTTCTAATACTACTATCTCCTTTCAACATGTTGTACACACATTTTTCAATAACAATATGTTGCAAGGTTGCCATAAGGCCCCTTATGATATGGCCAATTTTCAAAAGCCACGGCACTTGGTGGATTTCTTAAGGTTGTACTTTGTTCCCGAGAAGAGTGAAAATTGTCCACGCGATAGGTGCCTTCCCCCAAATGCAACCGCTCTTCGTGAGGCTGGTGTCGTCTTCAAGAAAGCAAAACGAGAGGAAAGACACATTATGGACATAAGGTTCAAAGATGGGGTCCTAGAAATTCCATCTTTCGGAATTGAAGATGATTTTGAAACATATGTACGAAACTTAATGGCATTTGAGCAGTTTTTGGTGGAGAATAATGAGGTGAAAAGTGAAGATCAGTGTCACAAGGAGCTTGATGAGAAGATTAGCAAAGAGAGCATTAAAAGGTATGCAACGGATTATTTTGAGTTCCTAGATGAGTTGATATCATCAGAGAAAGATGTGAGTTTACTTGTGAAGGAAGGAATCATAATCAATAGTATTGGTGGCAGTGATGAAGAAATTTCAAAATTGTTTAATGACCTTTGCAAGTATATCACCGTAGATGAATTTAACCACTTCTCCAATATTAGCGAAGATCTAAAAAAGCACTGTGAAAAACGATGGAACAGGTGGATGGCTTCATTGAGACACAACTATTTTAATACGCCATGGGCTTTTATTTCCTTCTTAGCTGCAACCTTCATTATTTTGCTCACTCTCCTACAAACCATATTCTCTGGTGTATCCATTTCCAAGTAA

Coding sequence (CDS)

ATGGATTGCGGTCATGTTGAAGCATATCACAATATTGATGAGGTTAAACGACAATCTCGGGATGAGATCACGGTATTCATCGAAGGAAAGCTTCAAGGAGTGCCTCGCACCGTTCCAACATATAGCCTCTATCGGGTTCCCAAACGGCTACTTGACATTAACCCTATAGCTTATGTGCCTCGAGTCATTTCAATTGGTCCTTTTCACCATCATCAACAGATTTTGAAGGACACAGAAGAGCTCAAGTTTCGATTTTTAAGTAGCTATCAACGTCGCGTAAATAAGAGCATTCAGGACATTGTGGAAAAGACTCGAAGTTGGGAGACAATAGCCCGTAAATGCTACTCAGAGCCCATAAACATGAACATTGATGACTTTGTGGGAATGATGGTTTTAGATGGTTGTTTCATAGTGGAGTTCATGATAATAAATTACGGTGACAATTTTCCCAAAAATGAAATGACGGTAAATTCTCCCTTGTATGAACCTATAAGATTTGATATATGTCGTGATTTGACAATTCTTGAGAACCAACTTCCTTTCTTTGTTCTACAAGGACTGTTTGACCTAGTTCCACTCCCTTCTAATACTACTATCTCCTTTCAACATGTTGTACACACATTTTTCAATAACAATATGTTGCAAGGTTGCCATAAGGCCCCTTATGATATGGCCAATTTTCAAAAGCCACGGCACTTGGTGGATTTCTTAAGGTTGTACTTTGTTCCCGAGAAGAGTGAAAATTGTCCACGCGATAGGTGCCTTCCCCCAAATGCAACCGCTCTTCGTGAGGCTGGTGTCGTCTTCAAGAAAGCAAAACGAGAGGAAAGACACATTATGGACATAAGGTTCAAAGATGGGGTCCTAGAAATTCCATCTTTCGGAATTGAAGATGATTTTGAAACATATGTACGAAACTTAATGGCATTTGAGCAGTTTTTGGTGGAGAATAATGAGGTGAAAAGTGAAGATCAGTGTCACAAGGAGCTTGATGAGAAGATTAGCAAAGAGAGCATTAAAAGGTATGCAACGGATTATTTTGAGTTCCTAGATGAGTTGATATCATCAGAGAAAGATGTGAGTTTACTTGTGAAGGAAGGAATCATAATCAATAGTATTGGTGGCAGTGATGAAGAAATTTCAAAATTGTTTAATGACCTTTGCAAGTATATCACCGTAGATGAATTTAACCACTTCTCCAATATTAGCGAAGATCTAAAAAAGCACTGTGAAAAACGATGGAACAGGTGGATGGCTTCATTGAGACACAACTATTTTAATACGCCATGGGCTTTTATTTCCTTCTTAGCTGCAACCTTCATTATTTTGCTCACTCTCCTACAAACCATATTCTCTGGTGTATCCATTTCCAAGTAA

Protein sequence

MDCGHVEAYHNIDEVKRQSRDEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNMLQGCHKAPYDMANFQKPRHLVDFLRLYFVPEKSENCPRDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVDEFNHFSNISEDLKKHCEKRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFSGVSISK
Homology
BLAST of Lcy13g014210 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.6e-40
Identity = 127/436 (29.13%), Postives = 219/436 (50.23%), Query Frame = 0

Query: 43  LYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEELKFRFLSSY-QRRVNKSIQD-- 102
           ++RVP+  + +NP AY P+V+SIGP+H+ ++ L+  ++ K R L  +      K +++  
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENV 107

Query: 103 IVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSP 162
           +V+     E   RK YSE +     D + MMVLDGCFI+   +I  G N   +E  + S 
Sbjct: 108 LVKAVVDLEDKIRKSYSEELKTG-HDLMFMMVLDGCFILMVFLIMSG-NIELSEDPIFSI 167

Query: 163 LYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNM-LQGCH 222
            +  +   I  DL +LENQ+PFFVLQ L+  V      +     +   FF N +  +G +
Sbjct: 168 PW--LLSSIQSDLLLLENQVPFFVLQTLY--VGSKIGVSSDLNRIAFHFFKNPIDKEGSY 227

Query: 223 KAPYDMANFQKPRHLVDFLRLYFVPEKSE-------------------NCPR--DRCLP- 282
              ++     K +HL+D +R  F+P  SE                   N P    + +P 
Sbjct: 228 ---WEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVDSKAVPL 287

Query: 283 -PNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRNLMAFEQFLV 342
             +A  LR  G+ F+  + +E  I+++R K   L+IP    +    ++  N +AFEQF  
Sbjct: 288 ILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQFYT 347

Query: 343 E-NNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGIIINSIG 402
           + +NE+                       T Y  F+  L+++E+DV+ L  + +II +  
Sbjct: 348 DSSNEI-----------------------TTYIVFMGCLLNNEEDVTFLRNDKLIIENHF 407

Query: 403 GSDEEISKLFNDLCKYITVD-EFNHFSNISEDLKKHCEKRWNRWMASLRHNYFNTPWAFI 450
           GS+ E+S+ F  + K +  + + ++ +N+ + + ++ +K +N   A  RH +F +PW F+
Sbjct: 408 GSNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFL 451

BLAST of Lcy13g014210 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.9e-23
Identity = 117/481 (24.32%), Postives = 203/481 (42.20%), Query Frame = 0

Query: 40  TYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNK-SIQ 99
           T S++ VPK L+  +P +Y P  +SIGP+H  +  L + E  K       + + N     
Sbjct: 42  TVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFH 101

Query: 100 DIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNS 159
           D+VEK +S E   R CY + I  N +  + +M +D  F++EF+ I    +F K E  +N 
Sbjct: 102 DLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIY---SFRKVETLINR 161

Query: 160 PLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNMLQGCH 219
             +     +I RD+ ++ENQ+P FVL+   +     + +       V T    ++     
Sbjct: 162 VGHN----EILRDIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVI 221

Query: 220 KAPYDM---ANFQKPRHLVDFLRLYFVPEKSE---------------------------- 279
           K   D    A FQ+  H++DFL    VP   E                            
Sbjct: 222 KFDDDQILKAQFQECNHILDFLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIK 281

Query: 280 --------NCPRDRCLP---------PNATALR-EAGVVFKK-------AKREERHIMDI 339
                   + P D  L          P   AL+  A  +F +        ++E   I+DI
Sbjct: 282 HQFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDI 341

Query: 340 RFKDGVLEIPSFGIEDDFETYVR---------NLMAFE----QFL--VENNEVKSEDQCH 399
                V E+    + D  +  VR         + + F+    QF   V N ++ +E    
Sbjct: 342 EKPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLR 401

Query: 400 KELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDL 449
             +  + +  S     T Y E ++ +I SE+DV LL ++G++++ +  SD+E ++++N +
Sbjct: 402 NLVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRL-KSDQEAAEMWNGM 461

BLAST of Lcy13g014210 vs. ExPASy TrEMBL
Match: A0A6J1BR71 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 3.7e-98
Identity = 216/467 (46.25%), Postives = 289/467 (61.88%), Query Frame = 0

Query: 1   MDCGHVEAY---HNIDEVKRQSRDEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIA 60
           M+  H+E Y     IDEV+ + +  +T+ ++  L+ +       S+YRV KRL +IN +A
Sbjct: 1   MEDDHIETYDLNKKIDEVELE-QPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMA 60

Query: 61  YVPRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSE 120
           Y P+ ISIGPFHH Q+     E+LK RFL +Y RRV   I+D  E  + WET ARKCY+E
Sbjct: 61  YTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAE 120

Query: 121 PINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILEN 180
            I+M  D+FV MM++DG F+VEF+ ++Y       +  +N  L++ I  DI RDL +LEN
Sbjct: 121 HIDMKSDNFVKMMLVDGAFLVEFIRMHY-QWATMTQPNLNYTLFQAIHVDIYRDLILLEN 180

Query: 181 QLPFFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNMLQGCHKAPYDMANFQKPRHLVDFL 240
           QLPFF+L+ L D        + S   V+ T        G  +   D    +KP HLVDFL
Sbjct: 181 QLPFFILECLLD------KCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFL 240

Query: 241 RLYF----VPEKSENCP-RDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIP 300
             Y+    V  K++      R  PP AT L EAGV F+KA  ++R IMDIRFKDGVL IP
Sbjct: 241 SFYYALPTVTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIP 300

Query: 301 SFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDE 360
              I D FETYVRNL+A+E + + ++E                     R    Y  FLDE
Sbjct: 301 HLEIHDAFETYVRNLLAYEHYHIGDDE---------------------RCLIQYVYFLDE 360

Query: 361 LISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVD-EFNHFSNISEDLKKHCE 420
           LIS+E+DVSLLVK GII N+IGG++E++SKLFNDLCK I +  +F ++++IS DL K+CE
Sbjct: 361 LISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCE 420

Query: 421 KRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
             W+R MASLR +YFNTPWAFISFLAATF++LLT +Q I+S +S  K
Sbjct: 421 TWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAISYHK 438

BLAST of Lcy13g014210 vs. ExPASy TrEMBL
Match: A0A6J1BQT6 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004764 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 4.1e-97
Identity = 216/462 (46.75%), Postives = 292/462 (63.20%), Query Frame = 0

Query: 11  NIDEVKRQSRDEITVFIEGKLQGVPRTV--PTYSLYRVPKRLLDINPIAYVPRVISIGPF 70
           N +EV  Q    + + IE   + +P     P  S+YRVPKRLL++N  AY P+VISIGPF
Sbjct: 25  NNNEVDEQHCRHVVISIEEMCKRLPPPPIDPECSIYRVPKRLLNMNRKAYTPQVISIGPF 84

Query: 71  HH-HQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFV 130
           HH +Q  L  T++ K + L SY  RV  +++ +V+ T++WE  AR CY EPI MN D FV
Sbjct: 85  HHSNQSNLIVTQQHKLQALDSYLHRVKMTVEAVVKITQNWENRARSCYGEPIKMNNDKFV 144

Query: 131 GMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGL 190
            M++LDGCF+V F+I++Y +N+  +E   +S  YE +  DI  D+T+LENQLPFFVLQGL
Sbjct: 145 TMLLLDGCFVVXFLILDY-NNYETDENGFDSSFYEAMSSDIYGDMTMLENQLPFFVLQGL 204

Query: 191 FDLVP----LPSNTTISFQHVVHTFFN---NNMLQGCHKAPYDMANFQKPRHLVDFLRLY 250
           +DL+P    + +N+ I    ++ TFF+   NN    CH +P ++      +HLVD L LY
Sbjct: 205 YDLIPKDHEIKNNSLI---QLIETFFSRSMNNHEIPCHVSPPNV------KHLVDLLSLY 264

Query: 251 FVP---EKSENCPRDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIE 310
           F+P    K ++   +  + P  T L EAGV  KK K E   +MDI FK+GVLEIP   I+
Sbjct: 265 FLPPCDTKQQHDKDEYLVTPCVTELCEAGVTIKKGK-EATCLMDISFKNGVLEIPPLDID 324

Query: 311 DDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSE 370
           D FET VRNLMAFE +   N                       RY   Y  FLD +IS+E
Sbjct: 325 DHFETIVRNLMAFEHYPAAN---------------------YHRYTIQYALFLDYMISTE 384

Query: 371 KDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVDEFNHFSN-ISEDLKKHCEKRWNR 430
           KDV LLV+ GIIINSIGGSD+E+S+LFNDL KY+++    H+ N I++ L  HC+K W R
Sbjct: 385 KDVRLLVEAGIIINSIGGSDKEVSRLFNDLGKYVSIPGGVHYLNHITKPLHDHCKKWWPR 444

Query: 431 WMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
             A+L+ +YFN+PWAFIS +AAT+II+LTLLQTIF+ +S  K
Sbjct: 445 SKATLKRDYFNSPWAFISIVAATYIIILTLLQTIFTAISTFK 454

BLAST of Lcy13g014210 vs. ExPASy TrEMBL
Match: A0A6J1DXD6 (UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 5.4e-97
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 29  DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 88

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 89  HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 148

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 149 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 208

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 209 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 268

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 269 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 328

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 329 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 388

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 389 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 448

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 449 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 452

BLAST of Lcy13g014210 vs. ExPASy TrEMBL
Match: A0A6J1E120 (UPF0481 protein At3g47200-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 5.4e-97
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 52  DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 111

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 112 HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 171

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 172 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 231

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 232 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 291

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 292 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 351

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 352 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 411

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 412 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 471

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 472 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 475

BLAST of Lcy13g014210 vs. ExPASy TrEMBL
Match: A0A6J1DYL4 (UPF0481 protein At3g47200-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 5.4e-97
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 7   DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 66

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 67  HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 126

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 127 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 186

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 187 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 246

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 247 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 306

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 307 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 366

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 367 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 426

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 427 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 430

BLAST of Lcy13g014210 vs. NCBI nr
Match: XP_022132066.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 368.6 bits (945), Expect = 7.7e-98
Identity = 216/467 (46.25%), Postives = 289/467 (61.88%), Query Frame = 0

Query: 1   MDCGHVEAY---HNIDEVKRQSRDEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIA 60
           M+  H+E Y     IDEV+ + +  +T+ ++  L+ +       S+YRV KRL +IN +A
Sbjct: 1   MEDDHIETYDLNKKIDEVELE-QPHVTISMKNMLEKLHPISEECSIYRVSKRLHNINDMA 60

Query: 61  YVPRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSE 120
           Y P+ ISIGPFHH Q+     E+LK RFL +Y RRV   I+D  E  + WET ARKCY+E
Sbjct: 61  YTPQAISIGPFHHGQKEFMAMEQLKLRFLDAYLRRVGMGIEDAFEIAQGWETRARKCYAE 120

Query: 121 PINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILEN 180
            I+M  D+FV MM++DG F+VEF+ ++Y       +  +N  L++ I  DI RDL +LEN
Sbjct: 121 HIDMKSDNFVKMMLVDGAFLVEFIRMHY-QWATMTQPNLNYTLFQAIHVDIYRDLILLEN 180

Query: 181 QLPFFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNMLQGCHKAPYDMANFQKPRHLVDFL 240
           QLPFF+L+ L D        + S   V+ T        G  +   D    +KP HLVDFL
Sbjct: 181 QLPFFILECLLD------KCSSSTPFVLFTSTFCRWYTGARELISDKLLTKKPNHLVDFL 240

Query: 241 RLYF----VPEKSENCP-RDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIP 300
             Y+    V  K++      R  PP AT L EAGV F+KA  ++R IMDIRFKDGVL IP
Sbjct: 241 SFYYALPTVTGKNDKLKYNKRESPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIP 300

Query: 301 SFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDE 360
              I D FETYVRNL+A+E + + ++E                     R    Y  FLDE
Sbjct: 301 HLEIHDAFETYVRNLLAYEHYHIGDDE---------------------RCLIQYVYFLDE 360

Query: 361 LISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVD-EFNHFSNISEDLKKHCE 420
           LIS+E+DVSLLVK GII N+IGG++E++SKLFNDLCK I +  +F ++++IS DL K+CE
Sbjct: 361 LISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDINISCDFYYYADISMDLHKYCE 420

Query: 421 KRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
             W+R MASLR +YFNTPWAFISFLAATF++LLT +Q I+S +S  K
Sbjct: 421 TWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIYSAISYHK 438

BLAST of Lcy13g014210 vs. NCBI nr
Match: XP_022131634.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 365.2 bits (936), Expect = 8.5e-97
Identity = 216/462 (46.75%), Postives = 292/462 (63.20%), Query Frame = 0

Query: 11  NIDEVKRQSRDEITVFIEGKLQGVPRTV--PTYSLYRVPKRLLDINPIAYVPRVISIGPF 70
           N +EV  Q    + + IE   + +P     P  S+YRVPKRLL++N  AY P+VISIGPF
Sbjct: 25  NNNEVDEQHCRHVVISIEEMCKRLPPPPIDPECSIYRVPKRLLNMNRKAYTPQVISIGPF 84

Query: 71  HH-HQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFV 130
           HH +Q  L  T++ K + L SY  RV  +++ +V+ T++WE  AR CY EPI MN D FV
Sbjct: 85  HHSNQSNLIVTQQHKLQALDSYLHRVKMTVEAVVKITQNWENRARSCYGEPIKMNNDKFV 144

Query: 131 GMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGL 190
            M++LDGCF+V F+I++Y +N+  +E   +S  YE +  DI  D+T+LENQLPFFVLQGL
Sbjct: 145 TMLLLDGCFVVXFLILDY-NNYETDENGFDSSFYEAMSSDIYGDMTMLENQLPFFVLQGL 204

Query: 191 FDLVP----LPSNTTISFQHVVHTFFN---NNMLQGCHKAPYDMANFQKPRHLVDFLRLY 250
           +DL+P    + +N+ I    ++ TFF+   NN    CH +P ++      +HLVD L LY
Sbjct: 205 YDLIPKDHEIKNNSLI---QLIETFFSRSMNNHEIPCHVSPPNV------KHLVDLLSLY 264

Query: 251 FVP---EKSENCPRDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIE 310
           F+P    K ++   +  + P  T L EAGV  KK K E   +MDI FK+GVLEIP   I+
Sbjct: 265 FLPPCDTKQQHDKDEYLVTPCVTELCEAGVTIKKGK-EATCLMDISFKNGVLEIPPLDID 324

Query: 311 DDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSE 370
           D FET VRNLMAFE +   N                       RY   Y  FLD +IS+E
Sbjct: 325 DHFETIVRNLMAFEHYPAAN---------------------YHRYTIQYALFLDYMISTE 384

Query: 371 KDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVDEFNHFSN-ISEDLKKHCEKRWNR 430
           KDV LLV+ GIIINSIGGSD+E+S+LFNDL KY+++    H+ N I++ L  HC+K W R
Sbjct: 385 KDVRLLVEAGIIINSIGGSDKEVSRLFNDLGKYVSIPGGVHYLNHITKPLHDHCKKWWPR 444

Query: 431 WMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
             A+L+ +YFN+PWAFIS +AAT+II+LTLLQTIF+ +S  K
Sbjct: 445 SKATLKRDYFNSPWAFISIVAATYIIILTLLQTIFTAISTFK 454

BLAST of Lcy13g014210 vs. NCBI nr
Match: XP_022158990.1 (UPF0481 protein At3g47200-like isoform X2 [Momordica charantia])

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-96
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 29  DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 88

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 89  HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 148

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 149 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 208

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 209 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 268

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 269 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 328

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 329 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 388

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 389 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 448

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 449 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 452

BLAST of Lcy13g014210 vs. NCBI nr
Match: XP_022158992.1 (UPF0481 protein At3g47200-like isoform X3 [Momordica charantia])

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-96
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 7   DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 66

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 67  HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 126

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 127 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 186

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 187 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 246

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 247 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 306

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 307 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 366

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 367 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 426

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 427 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 430

BLAST of Lcy13g014210 vs. NCBI nr
Match: XP_022158989.1 (UPF0481 protein At3g47200-like isoform X1 [Momordica charantia])

HSP 1 Score: 364.8 bits (935), Expect = 1.1e-96
Identity = 221/453 (48.79%), Postives = 280/453 (61.81%), Query Frame = 0

Query: 21  DEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEE 80
           DE+   I+  LQ +P      +++RVP+RLL  N  AY+P++ISIGPFHH +Q L   E+
Sbjct: 52  DEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQ 111

Query: 81  LKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE- 140
            K RFL  Y RR N  I+  V   RSWET AR CY+EPINM+ D+FV MM++DGCFIVE 
Sbjct: 112 HKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVEL 171

Query: 141 -FMIINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLVPLPSNTT 200
             M+   G    + E   +  L+  +  D+  DL +LENQLPFFVLQGLFD   L +   
Sbjct: 172 MMMVCRIGS---ETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEAG-- 231

Query: 201 ISFQHVVHTFFNNNML--QGCHKAPYD-MANFQKPRHLVDFLRLYFVP--------EKSE 260
           +SF  + H F+    L      + P+  M +  K  HLVDFL  Y+ P          S 
Sbjct: 232 LSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSL 291

Query: 261 NCPRDRC-LPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRN 320
              R +C  PP  T L EAG+VFKKA R  +HIMDI FKD VL+IP   I D FETYVRN
Sbjct: 292 AISRKKCTFPPTVTELWEAGIVFKKAMR-AKHIMDISFKDRVLQIPPLEIGDVFETYVRN 351

Query: 321 LMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKE 380
           LMAFEQ+   +N+ K                    YA  YF FL+ LIS E+DVSLLVK 
Sbjct: 352 LMAFEQY---HNDGK--------------------YAIQYFLFLNGLISREQDVSLLVKA 411

Query: 381 GIIINSIGGSDEEISKLFNDLCKYITV-DEFNHFSNISEDLKKHCEKRWNRWMASLRHNY 440
            II N IGG+++E+S LFNDLCK + V  + N F++I+E L +HC  RWN+ MASLR +Y
Sbjct: 412 NIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDY 471

Query: 441 FNTPWAFISFLAATFIILLTLLQTIFSGVSISK 459
           FNTPWAFISF+AA F+ILLT LQT+FS +S+SK
Sbjct: 472 FNTPWAFISFVAAAFLILLTFLQTLFSAMSLSK 475

BLAST of Lcy13g014210 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 239.2 bits (609), Expect = 6.6e-63
Identity = 153/469 (32.62%), Postives = 241/469 (51.39%), Query Frame = 0

Query: 3   CGHVEAYHNIDEVKRQSRDE---ITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYV 62
           C  V+ +      +R +++E   +   I+ KL  +        +Y+VP +L  +NP AY 
Sbjct: 252 CSEVQCHRLCFAYERMNQNEGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYT 311

Query: 63  PRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPI 122
           PR++S GP H  ++ L+  E+ K+R+L S+  R N S++D+V   R+WE  AR CY+E +
Sbjct: 312 PRLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDV 371

Query: 123 NMNIDDFVGMMVLDGCFIVEFMIINY-------GDNFPKNEMTVNSPLYEPIRFDICRDL 182
            ++ D+FV M+V+DG F+VE ++ ++        D    N M +          D+CRD+
Sbjct: 372 KLHSDEFVEMLVVDGSFLVELLLRSHYPRLRGENDRIFGNSMMIT---------DVCRDM 431

Query: 183 TILENQLPFFVLQGLFDLV------PLPSNTTISFQHVVHTFFNNNMLQGCHKAPYDMAN 242
            ++ENQLPFFV++ +F L+        PS   ++ +H   ++F + +         D   
Sbjct: 432 ILIENQLPFFVVKEIFLLLLNYYQQGTPSIIQLAQRH--FSYFLSRI--------DDEKF 491

Query: 243 FQKPRHLVDFLRLYFVPE---KSENCPRDRCLPPNATALREAGVVFKKAKREERHIMDIR 302
             +P H VD LR  ++P+   K E         P AT L  AGV FK A+     ++DI 
Sbjct: 492 ITEPEHFVDLLRSCYLPQFPIKLEYTTVKVDNAPEATELHTAGVRFKPAETSS-CLLDIS 551

Query: 303 FKDGVLEIPSFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESIKRYA 362
           F DGVL+IP+  ++D  E+  +N++ FEQ    N                          
Sbjct: 552 FADGVLKIPTIVVDDLTESLYKNIIGFEQCRCSNKNF----------------------- 611

Query: 363 TDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVDEFNHFSNIS 422
            DY   L   I S  D  LL+  GII+N +G S  ++S LFN + K +  D   +FS +S
Sbjct: 612 LDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNS-VDVSNLFNSISKEVIYDRRFYFSMLS 671

Query: 423 EDLKKHCEKRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFS 453
           E+L+ +C   WNRW A LR +YF+ PWA  S  AA  ++LLT +Q++ S
Sbjct: 672 ENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCS 676

BLAST of Lcy13g014210 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-57
Identity = 146/445 (32.81%), Postives = 241/445 (54.16%), Query Frame = 0

Query: 30  KLQGVPRTV------PTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEELKF 89
           KL+  PR +      PT S++RVP+ ++D N   Y PRV+SIGP+H  Q  LK  EE K+
Sbjct: 28  KLKEPPRLLSSAAGKPTCSIFRVPQSMIDCNGRCYEPRVVSIGPYHRGQTQLKMIEEHKW 87

Query: 90  RFLSSYQRRV-NKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVE-FM 149
           R+L+    R  N +++D ++  ++ E +AR+CYSE I+M+ ++F  MMVLDGCF++E F 
Sbjct: 88  RYLNVLLTRTQNLTLEDYMKSVKNVEEVARECYSETIHMDSEEFNEMMVLDGCFLLELFR 147

Query: 150 IINYGDNFPKNEMTVNSPLYEPIRFDICRDLTILENQLPFFVLQGLFDLV--PLPSNTTI 209
            +N    F  N+  V      P  +   RD   LENQ+PFFVL+ LF+L      + T  
Sbjct: 148 KVNNLVPFEPNDPLVAMAWVLPFFY---RDFLCLENQIPFFVLETLFNLTRGDNENETNA 207

Query: 210 SFQHVVHTFFNNNMLQGCHKAPYDMANFQ--KPRHLVDFLRLYFVPEKSENCP------- 269
           S Q +   FFNN M    H+   D+A F+  + +HL+D LR  F+PE   + P       
Sbjct: 208 SLQSLAFAFFNNMM----HRTEEDLARFKELRAKHLLDLLRSSFIPESELHTPPATNPGK 267

Query: 270 --RDRCLPPNATALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRNLM 329
                 +  + + LR AG+  ++ K  E  ++ +RF+ G +E+P+  ++D   +++ N +
Sbjct: 268 EKMPSHIIHSISKLRRAGIKLRELKDAESFLV-VRFRHGTIEMPAITVDDFMSSFLENCV 327

Query: 330 AFEQFLVENNEVKSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGI 389
           A+E             QCH          +   + T Y   LD L ++ KDV  L  + I
Sbjct: 328 AYE-------------QCH---------VACSMHFTTYATLLDCLTNTYKDVEYLCDQNI 387

Query: 390 IINSIGGSDEEISKLFNDLCKYITVDEFN-HFSNISEDLKKHCEKRWNRWMASLRHNYFN 449
           I N   G+D E++K  N L + +  D    +  ++ E++ ++ +  W+   A+ +  YFN
Sbjct: 388 IENYF-GTDTELAKFVNSLGRDVAFDITQCYLKDLFEEVNEYYKSSWHVEWATFKFTYFN 441

Query: 450 TPWAFISFLAATFIILLTLLQTIFS 453
           +PW+F+S LAA  +++L+++QTI++
Sbjct: 448 SPWSFVSALAALVLLVLSVIQTIYT 441

BLAST of Lcy13g014210 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 210.7 bits (535), Expect = 2.5e-54
Identity = 155/474 (32.70%), Postives = 231/474 (48.73%), Query Frame = 0

Query: 13  DEVKRQSRDEITVFIEGKLQGVPRTVPTYSLYRVPKRLLDINPIAYVPRVISIGPFHHHQ 72
           D++++  RD+ T  I GKL           +YRVP  L + +  +Y P+ +S+GP+HH +
Sbjct: 96  DKLEQADRDDDTT-IWGKL----------CIYRVPHYLQENDKKSYFPQTVSLGPYHHGK 155

Query: 73  QILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMNIDDFVGMMVL 132
           + L+  E  K+R L+   +R+ + I+      R  E  AR CY  PI+++ ++F  M+VL
Sbjct: 156 KRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACYEGPISLSRNEFTEMLVL 215

Query: 133 DGCFIVEFMIINYGDNFPKNEMTVNSPLY--EPIRFDICRDLTILENQLPFFVLQGLFDL 192
           DGCF++E +     + F +     N P++    +   I RD+ +LENQLP FVL  L +L
Sbjct: 216 DGCFVLE-LFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLEL 275

Query: 193 VPLPSNTTISFQHVVHTFF-------------NNNMLQGCHKAPYDMANFQKPRHLVDFL 252
                N T    HV   FF             + + L    +   D    +   H +D  
Sbjct: 276 QLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVF 335

Query: 253 RLYFVPEKSENCPRDRCLPPN------------------ATALREAGVVFKKAKREERHI 312
           R   +  +S   P  R L                      T LREAGV F+K K +    
Sbjct: 336 RRSLL--QSSPTPNTRSLLKRLTRNTRVVDKRQQQLVHCVTELREAGVKFRKRKTD--RF 395

Query: 313 MDIRFKDGVLEIPSFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKELDEKISKESI 372
            DI FK+G LEIP   I D  ++   NL+AFE             QCH E    I     
Sbjct: 396 WDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFE-------------QCHIESSNHI----- 455

Query: 373 KRYATDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCKYITVD-EFNH 432
               T Y  F+D LI+S +DVS L   GII + + GSD E++ LFN LC+ +  D + +H
Sbjct: 456 ----TSYIIFMDNLINSSEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQEVVFDPKDSH 515

Query: 433 FSNISEDLKKHCEKRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQTIFS 453
            S +S D+ ++  ++WN   A+L H YFN PWA+ SF AA  ++LLTL Q+ ++
Sbjct: 516 LSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYA 530

BLAST of Lcy13g014210 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 205.7 bits (522), Expect = 8.0e-53
Identity = 150/484 (30.99%), Postives = 237/484 (48.97%), Query Frame = 0

Query: 8   AYHNIDEVKRQSRDEITVFIEGKLQGVPRTVPT-----YSLYRVPKRLLDINPIAYVPRV 67
           A + I E  + SRD+  + I  KL+   R   T       +YRVP  L + +  +Y P+ 
Sbjct: 65  AIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQT 124

Query: 68  ISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEKTRSWETIARKCYSEPINMN 127
           +S+GP+HH ++ L+  +  K+R ++   +R N+ I+  ++  R  E  AR CY  P++++
Sbjct: 125 VSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGPLSLS 184

Query: 128 IDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLY--EPIRFDICRDLTILENQLP 187
            ++F+ M+VLDGCF++E +     + F +     N P++        I RD+ +LENQLP
Sbjct: 185 SNEFIEMLVLDGCFVLE-LFRGAVEGFTELGYARNDPVFAMRGSMHSIQRDMVMLENQLP 244

Query: 188 FFVLQGLFDLVPLPSNTTISFQHVVHTFFNNNM-------LQGCHKAPYDMANFQK---- 247
            FVL  L +L     N T     +   FF+  M         G  K    +A  +     
Sbjct: 245 LFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSFDPF 304

Query: 248 ----PRHLVDFLR---LYFVPEKSENCPRDRC-------------LPPNATALREAGVVF 307
                 H +D  R   L   P+      R R              L    T L+EAG+ F
Sbjct: 305 ADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVADKRRQQLIHCVTELKEAGIKF 364

Query: 308 KKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRNLMAFEQFLVENNEVKSEDQCHKE 367
           ++ K +     D++FK+G LEIP   I D  ++   NL+AFE             QCH +
Sbjct: 365 RRRKTD--RFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFE-------------QCHID 424

Query: 368 LDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEISKLFNDLCK 427
               I         T Y  F+D LI S +DVS L   GII + + GSD E++ LFN LC+
Sbjct: 425 SSNDI---------TSYIIFMDNLIDSHEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQ 484

Query: 428 YITVD-EFNHFSNISEDLKKHCEKRWNRWMASLRHNYFNTPWAFISFLAATFIILLTLLQ 453
            +  D E ++ S +S ++ ++ + +WN W A+L+H YFN PWA +SF AA  +++LT  Q
Sbjct: 485 EVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQ 522

BLAST of Lcy13g014210 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-51
Identity = 153/493 (31.03%), Postives = 233/493 (47.26%), Query Frame = 0

Query: 4   GHVEAYHNIDEVKRQSRDEITVFIEGKLQGVPRTVPTYSL-------------------- 63
           G +E    I+E  R+++ E  V IE K +   R +   SL                    
Sbjct: 47  GELENIEVIEEKPRETQVESVVSIEDKNEQKLREIWVISLNDKMKTLGDNATTSWDNLCI 106

Query: 64  YRVPKRLLDINPIAYVPRVISIGPFHHHQQILKDTEELKFRFLSSYQRRVNKSIQDIVEK 123
           YRVP  L + +  +Y+P+++SIGP+HH  + L   E  K+R ++    R    I+  ++ 
Sbjct: 107 YRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDA 166

Query: 124 TRSWETIARKCYSEPINMNIDDFVGMMVLDGCFIVEFMIINYGDNFPKNEMTVNSPLY-- 183
            +  E  AR CY  PINMN ++F+ M+VLDG FI+E +     + F +     N P++  
Sbjct: 167 MKELEEKARACYQGPINMNRNEFIEMLVLDGVFIIE-IFKGTSEGFQEIGYAPNDPVFGM 226

Query: 184 EPIRFDICRDLTILENQLPFFVLQGLFDLVP-------------------LPSNTTISFQ 243
             +   I RD+ +LENQLP+ VL+GL  L                     LP+   ++ +
Sbjct: 227 RGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQPLLPTREVLTEE 286

Query: 244 HVVHTF--FNNNMLQGCHKAPYDMANFQKPRHLVDFLRLYFVPEKSENCPRDRCLPPNAT 303
             +H        +LQ    +  DM+   K             P++  +C          T
Sbjct: 287 GGLHCLDVLRRGLLQSSGTSDEDMSMVNKQ------------PQQLIHC---------VT 346

Query: 304 ALREAGVVFKKAKREERHIMDIRFKDGVLEIPSFGIEDDFETYVRNLMAFEQFLVENNEV 363
            LR AGV F   ++E  H  DI FK+G L+IP   I D  ++   NL+AFE         
Sbjct: 347 ELRNAGVEF--MRKETGHFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFE--------- 406

Query: 364 KSEDQCHKELDEKISKESIKRYATDYFEFLDELISSEKDVSLLVKEGIIINSIGGSDEEI 423
               QCH +  +KI         T Y  F+D LI+S +DVS L   GII N + GSD E+
Sbjct: 407 ----QCHIKSSKKI---------TSYIIFMDNLINSSEDVSYLHHYGIIENWL-GSDSEV 466

Query: 424 SKLFNDLCKYITVD-EFNHFSNISEDLKKHCEKRWNRWMASLRHNYFNTPWAFISFLAAT 453
           S LFN L K +  D    + S ++ ++  +  ++WN   A+LRH YFN PWA+ SF+AA 
Sbjct: 467 SDLFNGLGKEVIFDPNDGYLSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAV 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD532.6e-4029.13UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8972.9e-2324.32Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BR713.7e-9846.25UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE... [more]
A0A6J1BQT64.1e-9746.75UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004764 PE... [more]
A0A6J1DXD65.4e-9748.79UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1E1205.4e-9748.79UPF0481 protein At3g47200-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DYL45.4e-9748.79UPF0481 protein At3g47200-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_022132066.17.7e-9846.25UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022131634.18.5e-9746.75UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022158990.11.1e-9648.79UPF0481 protein At3g47200-like isoform X2 [Momordica charantia][more]
XP_022158992.11.1e-9648.79UPF0481 protein At3g47200-like isoform X3 [Momordica charantia][more]
XP_022158989.11.1e-9648.79UPF0481 protein At3g47200-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G31980.16.6e-6332.62unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT2G36430.12.4e-5732.81Plant protein of unknown function (DUF247) [more]
AT3G50170.12.5e-5432.70Plant protein of unknown function (DUF247) [more]
AT3G50120.18.0e-5330.99Plant protein of unknown function (DUF247) [more]
AT3G50160.11.2e-5131.03Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 44..444
e-value: 6.4E-105
score: 351.7
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 20..454
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 20..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g014210.1Lcy13g014210.1mRNA